The Experts below are selected from a list of 462 Experts worldwide ranked by ideXlab platform

J. Freney - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of the new Vitek 2 GN card for the identification of Gram-Negative Bacilli frequently encountered in clinical laboratories.
    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2005
    Co-Authors: F. N. R. Renaud, E. Bergeron, S. Tigaud, C. Fuhrmann, B. Gravagna, J. Freney
    Abstract:

    The new Vitek 2 GN card (bioMerieux, Marcy-l'Etoile, France) was developed for better identification of fermenting and Nonfermenting Bacilli. This new card allows the identification of 159 taxa. A total of 426 isolates (331 fermenting and 95 Nonfermenting Gram-Negative Bacilli) belonging to 70 taxa covered by the database were evaluated. All isolates were identified in parallel with the ID 32 GN, the API 20E, and the API 20NE methods. The system correctly identified 97.4% (n=415) of the strains. Only 2.1% (n=9) needed additional testing. One strain (0.25%) was misidentified (Klebsiella pneumoniae subsp. pneumoniae), and another one (0.25%) was not identified (Morganella morganii subsp. morganii). The new GN card gives more accurate identifications overall for Gram-Negative Bacilli when compared to the systems described in other similar studies.

  • Evaluation of the new Vitek 2 GN card for the identification of Gram-Negative Bacilli frequently encountered in clinical laboratories
    European Journal of Clinical Microbiology and Infectious Diseases, 2005
    Co-Authors: F. N. R. Renaud, E. Bergeron, S. Tigaud, C. Fuhrmann, B. Gravagna, J. Freney
    Abstract:

    The new Vitek 2 GN card (bioMérieux, Marcy-l'Etoile, France) was developed for better identification of fermenting and Nonfermenting Bacilli. This new card allows the identification of 159 taxa. A total of 426 isolates (331 fermenting and 95 Nonfermenting Gram-Negative Bacilli) belonging to 70 taxa covered by the database were evaluated. All isolates were identified in parallel with the ID 32 GN, the API 20E, and the API 20NE methods. The system correctly identified 97.4% ( n =415) of the strains. Only 2.1% ( n =9) needed additional testing. One strain (0.25%) was misidentified ( Klebsiella pneumoniae subsp. pneumoniae ), and another one (0.25%) was not identified ( Morganella morganii subsp. morganii ). The new GN card gives more accurate identifications overall for Gram-Negative Bacilli when compared to the systems described in other similar studies.

  • Evaluation of the new Vitek 2 GN card for the identification of Gram-Negative Bacilli frequently encountered in clinical laboratories
    European Journal of Clinical Microbiology and Infectious Diseases, 2005
    Co-Authors: F. N. R. Renaud, E. Bergeron, S. Tigaud, C. Fuhrmann, B. Gravagna, J. Freney
    Abstract:

    The new Vitek 2 GN card (bioMérieux, Marcy-l'Etoile, France) was developed for better identification of fermenting and Nonfermenting Bacilli. This new card allows the identification of 159 taxa. A total of 426 isolates (331 fermenting and 95 Nonfermenting Gram-Negative Bacilli) belonging to 70 taxa covered by the database were evaluated. All isolates were identified in parallel with the ID 32 GN, the API 20E, and the API 20NE methods. The system correctly identified 97.4% ( n =415) of the strains. Only 2.1% ( n =9) needed additional testing. One strain (0.25%) was misidentified ( Klebsiella pneumoniae subsp. pneumoniae ), and another one (0.25%) was not identified ( Morganella morganii subsp. morganii ). The new GN card gives more accurate identifications overall for Gram-Negative Bacilli when compared to the systems described in other similar studies.

Franklin R Cockerill - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of the bruker biotyper and vitek ms matrix assisted laser desorption ionization time of flight mass spectrometry systems for identification of Nonfermenting gram negative Bacilli isolated from cultures from cystic fibrosis patients
    Journal of Clinical Microbiology, 2012
    Co-Authors: Daniel C Marko, Ryan T Saffert, Scott A Cunningham, Jay M Hyman, John Walsh, Sophie S Arbefeville, Wanita J Howard, Jon Pruessner, Nedal Safwat, Franklin R Cockerill
    Abstract:

    The Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry (MS) instruments were evaluated for the identification of Nonfermenting Gram-Negative Bacilli (NFGNB) by a blinded comparison to conventional biochemical or molecular methods. Two hundred NFGNB that were recovered from cultures from cystic fibrosis patients in the University of Iowa Health Care (UIHC) Microbiology Laboratory between 1 January 2006 and 31 October 2010 were sent to Mayo Clinic for analysis with the Bruker Biotyper (software version 3.0) and to bioMerieux for testing with Vitek MS (SARAMIS database version 3.62). If two attempts at direct colony testing failed to provide an acceptable MALDI-TOF identification, an extraction procedure was performed. The MS identifications from both of these systems were provided to UIHC for comparison to the biochemical or molecular identification that had been reported in the patient record. Isolates with discordant results were analyzed by 16S rRNA gene sequencing at UIHC. After discrepancy testing, the Bruker Biotyper result agreed with the biochemical or molecular method, with 72.5% of isolates to the species level, 5.5% to the complex level, and 19% to the genus level (3% not identified). The level of agreement for Vitek MS was 80% species, 3.5% complex, 6% genus, and 3.5% family (7% not identified). Both MS systems provided rapid (≤3 min per isolate) and reliable identifications. The agreement of combined species/complex/genus-level identification with the reference method was higher for the Bruker Biotyper (97% versus 89.5%, P = 0.004) but required an extraction step more often. Species-level agreement with the reference method was similar for both MS systems (72.5% and 80%, P = 0.099).

  • Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Nonfermenting Gram-Negative Bacilli Isolated from Cultures from Cystic Fibrosis Patients
    Journal of clinical microbiology, 2012
    Co-Authors: Daniel C Marko, Ryan T Saffert, Scott A Cunningham, Jay M Hyman, John Walsh, Sophie S Arbefeville, Wanita J Howard, Jon Pruessner, Nedal Safwat, Franklin R Cockerill
    Abstract:

    The Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry (MS) instruments were evaluated for the identification of Nonfermenting Gram-Negative Bacilli (NFGNB) by a blinded comparison to conventional biochemical or molecular methods. Two hundred NFGNB that were recovered from cultures from cystic fibrosis patients in the University of Iowa Health Care (UIHC) Microbiology Laboratory between 1 January 2006 and 31 October 2010 were sent to Mayo Clinic for analysis with the Bruker Biotyper (software version 3.0) and to bioMerieux for testing with Vitek MS (SARAMIS database version 3.62). If two attempts at direct colony testing failed to provide an acceptable MALDI-TOF identification, an extraction procedure was performed. The MS identifications from both of these systems were provided to UIHC for comparison to the biochemical or molecular identification that had been reported in the patient record. Isolates with discordant results were analyzed by 16S rRNA gene sequencing at UIHC. After discrepancy testing, the Bruker Biotyper result agreed with the biochemical or molecular method, with 72.5% of isolates to the species level, 5.5% to the complex level, and 19% to the genus level (3% not identified). The level of agreement for Vitek MS was 80% species, 3.5% complex, 6% genus, and 3.5% family (7% not identified). Both MS systems provided rapid (≤3 min per isolate) and reliable identifications. The agreement of combined species/complex/genus-level identification with the reference method was higher for the Bruker Biotyper (97% versus 89.5%, P = 0.004) but required an extraction step more often. Species-level agreement with the reference method was similar for both MS systems (72.5% and 80%, P = 0.099).

F. N. R. Renaud - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of the new Vitek 2 GN card for the identification of Gram-Negative Bacilli frequently encountered in clinical laboratories.
    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2005
    Co-Authors: F. N. R. Renaud, E. Bergeron, S. Tigaud, C. Fuhrmann, B. Gravagna, J. Freney
    Abstract:

    The new Vitek 2 GN card (bioMerieux, Marcy-l'Etoile, France) was developed for better identification of fermenting and Nonfermenting Bacilli. This new card allows the identification of 159 taxa. A total of 426 isolates (331 fermenting and 95 Nonfermenting Gram-Negative Bacilli) belonging to 70 taxa covered by the database were evaluated. All isolates were identified in parallel with the ID 32 GN, the API 20E, and the API 20NE methods. The system correctly identified 97.4% (n=415) of the strains. Only 2.1% (n=9) needed additional testing. One strain (0.25%) was misidentified (Klebsiella pneumoniae subsp. pneumoniae), and another one (0.25%) was not identified (Morganella morganii subsp. morganii). The new GN card gives more accurate identifications overall for Gram-Negative Bacilli when compared to the systems described in other similar studies.

  • Evaluation of the new Vitek 2 GN card for the identification of Gram-Negative Bacilli frequently encountered in clinical laboratories
    European Journal of Clinical Microbiology and Infectious Diseases, 2005
    Co-Authors: F. N. R. Renaud, E. Bergeron, S. Tigaud, C. Fuhrmann, B. Gravagna, J. Freney
    Abstract:

    The new Vitek 2 GN card (bioMérieux, Marcy-l'Etoile, France) was developed for better identification of fermenting and Nonfermenting Bacilli. This new card allows the identification of 159 taxa. A total of 426 isolates (331 fermenting and 95 Nonfermenting Gram-Negative Bacilli) belonging to 70 taxa covered by the database were evaluated. All isolates were identified in parallel with the ID 32 GN, the API 20E, and the API 20NE methods. The system correctly identified 97.4% ( n =415) of the strains. Only 2.1% ( n =9) needed additional testing. One strain (0.25%) was misidentified ( Klebsiella pneumoniae subsp. pneumoniae ), and another one (0.25%) was not identified ( Morganella morganii subsp. morganii ). The new GN card gives more accurate identifications overall for Gram-Negative Bacilli when compared to the systems described in other similar studies.

  • Evaluation of the new Vitek 2 GN card for the identification of Gram-Negative Bacilli frequently encountered in clinical laboratories
    European Journal of Clinical Microbiology and Infectious Diseases, 2005
    Co-Authors: F. N. R. Renaud, E. Bergeron, S. Tigaud, C. Fuhrmann, B. Gravagna, J. Freney
    Abstract:

    The new Vitek 2 GN card (bioMérieux, Marcy-l'Etoile, France) was developed for better identification of fermenting and Nonfermenting Bacilli. This new card allows the identification of 159 taxa. A total of 426 isolates (331 fermenting and 95 Nonfermenting Gram-Negative Bacilli) belonging to 70 taxa covered by the database were evaluated. All isolates were identified in parallel with the ID 32 GN, the API 20E, and the API 20NE methods. The system correctly identified 97.4% ( n =415) of the strains. Only 2.1% ( n =9) needed additional testing. One strain (0.25%) was misidentified ( Klebsiella pneumoniae subsp. pneumoniae ), and another one (0.25%) was not identified ( Morganella morganii subsp. morganii ). The new GN card gives more accurate identifications overall for Gram-Negative Bacilli when compared to the systems described in other similar studies.

Scott A Cunningham - One of the best experts on this subject based on the ideXlab platform.

  • Multicenter Performance Assessment of Carba NP Test.
    Journal of clinical microbiology, 2017
    Co-Authors: Scott A Cunningham, Brandi Limbago, Maria M. Traczewski, Karen F. Anderson, Meredith Hackel, Janet A. Hindler, Dan Sahm, Efe Alyanak, Adrian Lawsin, Christopher A. Gulvik
    Abstract:

    Eighty Gram-Negative Bacilli (54 Enterobacteriaceae and 26 Nonfermenting Gram-Negative Bacilli) obtained from multiple institutions in the United States were distributed in a blinded manner to seven testing laboratories to compare their performance of a test for detection of carbapenemase production, the Carba NP test. The Carba NP test was performed by all laboratories, following the Clinical and Laboratory Standards Institute (CLSI) procedure. Site-versus-site comparisons demonstrated a high level of consistency for the Carba NP assay, with just 3/21 site comparisons yielding a difference in sensitivity (P < 0.05). Previously described limitations with blaOXA-48-like carbapenemases and blaOXA carbapenemases associated with Acinetobacter baumannii were noted. Based on these data, we demonstrate that the Carba NP test, when implemented with the standardized CLSI methodology, provides reproducible results across multiple sites for detection of carbapenemases.

  • evaluation of the bruker biotyper and vitek ms matrix assisted laser desorption ionization time of flight mass spectrometry systems for identification of Nonfermenting gram negative Bacilli isolated from cultures from cystic fibrosis patients
    Journal of Clinical Microbiology, 2012
    Co-Authors: Daniel C Marko, Ryan T Saffert, Scott A Cunningham, Jay M Hyman, John Walsh, Sophie S Arbefeville, Wanita J Howard, Jon Pruessner, Nedal Safwat, Franklin R Cockerill
    Abstract:

    The Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry (MS) instruments were evaluated for the identification of Nonfermenting Gram-Negative Bacilli (NFGNB) by a blinded comparison to conventional biochemical or molecular methods. Two hundred NFGNB that were recovered from cultures from cystic fibrosis patients in the University of Iowa Health Care (UIHC) Microbiology Laboratory between 1 January 2006 and 31 October 2010 were sent to Mayo Clinic for analysis with the Bruker Biotyper (software version 3.0) and to bioMerieux for testing with Vitek MS (SARAMIS database version 3.62). If two attempts at direct colony testing failed to provide an acceptable MALDI-TOF identification, an extraction procedure was performed. The MS identifications from both of these systems were provided to UIHC for comparison to the biochemical or molecular identification that had been reported in the patient record. Isolates with discordant results were analyzed by 16S rRNA gene sequencing at UIHC. After discrepancy testing, the Bruker Biotyper result agreed with the biochemical or molecular method, with 72.5% of isolates to the species level, 5.5% to the complex level, and 19% to the genus level (3% not identified). The level of agreement for Vitek MS was 80% species, 3.5% complex, 6% genus, and 3.5% family (7% not identified). Both MS systems provided rapid (≤3 min per isolate) and reliable identifications. The agreement of combined species/complex/genus-level identification with the reference method was higher for the Bruker Biotyper (97% versus 89.5%, P = 0.004) but required an extraction step more often. Species-level agreement with the reference method was similar for both MS systems (72.5% and 80%, P = 0.099).

  • Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Nonfermenting Gram-Negative Bacilli Isolated from Cultures from Cystic Fibrosis Patients
    Journal of clinical microbiology, 2012
    Co-Authors: Daniel C Marko, Ryan T Saffert, Scott A Cunningham, Jay M Hyman, John Walsh, Sophie S Arbefeville, Wanita J Howard, Jon Pruessner, Nedal Safwat, Franklin R Cockerill
    Abstract:

    The Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry (MS) instruments were evaluated for the identification of Nonfermenting Gram-Negative Bacilli (NFGNB) by a blinded comparison to conventional biochemical or molecular methods. Two hundred NFGNB that were recovered from cultures from cystic fibrosis patients in the University of Iowa Health Care (UIHC) Microbiology Laboratory between 1 January 2006 and 31 October 2010 were sent to Mayo Clinic for analysis with the Bruker Biotyper (software version 3.0) and to bioMerieux for testing with Vitek MS (SARAMIS database version 3.62). If two attempts at direct colony testing failed to provide an acceptable MALDI-TOF identification, an extraction procedure was performed. The MS identifications from both of these systems were provided to UIHC for comparison to the biochemical or molecular identification that had been reported in the patient record. Isolates with discordant results were analyzed by 16S rRNA gene sequencing at UIHC. After discrepancy testing, the Bruker Biotyper result agreed with the biochemical or molecular method, with 72.5% of isolates to the species level, 5.5% to the complex level, and 19% to the genus level (3% not identified). The level of agreement for Vitek MS was 80% species, 3.5% complex, 6% genus, and 3.5% family (7% not identified). Both MS systems provided rapid (≤3 min per isolate) and reliable identifications. The agreement of combined species/complex/genus-level identification with the reference method was higher for the Bruker Biotyper (97% versus 89.5%, P = 0.004) but required an extraction step more often. Species-level agreement with the reference method was similar for both MS systems (72.5% and 80%, P = 0.099).

Daniel C Marko - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of the bruker biotyper and vitek ms matrix assisted laser desorption ionization time of flight mass spectrometry systems for identification of Nonfermenting gram negative Bacilli isolated from cultures from cystic fibrosis patients
    Journal of Clinical Microbiology, 2012
    Co-Authors: Daniel C Marko, Ryan T Saffert, Scott A Cunningham, Jay M Hyman, John Walsh, Sophie S Arbefeville, Wanita J Howard, Jon Pruessner, Nedal Safwat, Franklin R Cockerill
    Abstract:

    The Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry (MS) instruments were evaluated for the identification of Nonfermenting Gram-Negative Bacilli (NFGNB) by a blinded comparison to conventional biochemical or molecular methods. Two hundred NFGNB that were recovered from cultures from cystic fibrosis patients in the University of Iowa Health Care (UIHC) Microbiology Laboratory between 1 January 2006 and 31 October 2010 were sent to Mayo Clinic for analysis with the Bruker Biotyper (software version 3.0) and to bioMerieux for testing with Vitek MS (SARAMIS database version 3.62). If two attempts at direct colony testing failed to provide an acceptable MALDI-TOF identification, an extraction procedure was performed. The MS identifications from both of these systems were provided to UIHC for comparison to the biochemical or molecular identification that had been reported in the patient record. Isolates with discordant results were analyzed by 16S rRNA gene sequencing at UIHC. After discrepancy testing, the Bruker Biotyper result agreed with the biochemical or molecular method, with 72.5% of isolates to the species level, 5.5% to the complex level, and 19% to the genus level (3% not identified). The level of agreement for Vitek MS was 80% species, 3.5% complex, 6% genus, and 3.5% family (7% not identified). Both MS systems provided rapid (≤3 min per isolate) and reliable identifications. The agreement of combined species/complex/genus-level identification with the reference method was higher for the Bruker Biotyper (97% versus 89.5%, P = 0.004) but required an extraction step more often. Species-level agreement with the reference method was similar for both MS systems (72.5% and 80%, P = 0.099).

  • Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Nonfermenting Gram-Negative Bacilli Isolated from Cultures from Cystic Fibrosis Patients
    Journal of clinical microbiology, 2012
    Co-Authors: Daniel C Marko, Ryan T Saffert, Scott A Cunningham, Jay M Hyman, John Walsh, Sophie S Arbefeville, Wanita J Howard, Jon Pruessner, Nedal Safwat, Franklin R Cockerill
    Abstract:

    The Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry (MS) instruments were evaluated for the identification of Nonfermenting Gram-Negative Bacilli (NFGNB) by a blinded comparison to conventional biochemical or molecular methods. Two hundred NFGNB that were recovered from cultures from cystic fibrosis patients in the University of Iowa Health Care (UIHC) Microbiology Laboratory between 1 January 2006 and 31 October 2010 were sent to Mayo Clinic for analysis with the Bruker Biotyper (software version 3.0) and to bioMerieux for testing with Vitek MS (SARAMIS database version 3.62). If two attempts at direct colony testing failed to provide an acceptable MALDI-TOF identification, an extraction procedure was performed. The MS identifications from both of these systems were provided to UIHC for comparison to the biochemical or molecular identification that had been reported in the patient record. Isolates with discordant results were analyzed by 16S rRNA gene sequencing at UIHC. After discrepancy testing, the Bruker Biotyper result agreed with the biochemical or molecular method, with 72.5% of isolates to the species level, 5.5% to the complex level, and 19% to the genus level (3% not identified). The level of agreement for Vitek MS was 80% species, 3.5% complex, 6% genus, and 3.5% family (7% not identified). Both MS systems provided rapid (≤3 min per isolate) and reliable identifications. The agreement of combined species/complex/genus-level identification with the reference method was higher for the Bruker Biotyper (97% versus 89.5%, P = 0.004) but required an extraction step more often. Species-level agreement with the reference method was similar for both MS systems (72.5% and 80%, P = 0.099).