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Antonio L. Crego - One of the best experts on this subject based on the ideXlab platform.
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Potential of vancomycin for the enantiomeric resolution of FMOC‐Amino Acids by capillary electrophoresis‐ion‐trap‐mass spectrometry
Electrophoresis, 2014Co-Authors: Laura Sánchez-hernández, María Castro-puyana, María Luisa Marina, Elena Domínguez-vega, Cristina Montealegre, Antonio L. CregoAbstract:The potential of the antibiotic vancomycin (VC) as chiral selector for the enantiomeric separation of Amino Acids by CE-ESI-MS/MS² was investigated for the first time in this work. Derivatization of Amino Acids with FMOC-Cl was carried out to enable their interaction with VC as well as the formation of precursor ions with larger m/z which were employed in MS² experiments. The partial filling of a coated capillary was employed to avoid the loss in MS sensitivity originated by the introduction of VC in the ionization source. Under optimized conditions, the simultaneous enantiomeric separation and unequivocal identification of 17 Amino Acids (two of them being Nonprotein Amino Acids) took place in about 20 min with LODs in the micromolar range.
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Determination of Nonprotein Amino Acids and betaines in vegetable oils by flow injection triple-quadrupole tandem mass spectrometry: a screening method for the detection of adulterations of olive oils.
Journal of agricultural and food chemistry, 2012Co-Authors: Laura Sánchez-hernández, María Luisa Marina, Leonor Nozal, Antonio L. CregoAbstract:A novel screening method using an automated flow injection electrospray ionization tandem mass spectrometry system is proposed for the simultaneous determination of five Nonprotein Amino Acids (β-alanine, alloisoleucine, ornithine, citrulline, pyroglutamic acid) and three betaines (glycine betaine, trigonelline, proline betaine) after derivatization with butanolic HCl. MS/MS experiments were carried out in a triple-quadrupole instrument using multiple reaction monitoring mode in
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determination of Nonprotein Amino Acids and betaines in vegetable oils by flow injection triple quadrupole tandem mass spectrometry a screening method for the detection of adulterations of olive oils
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Laura Sanchezhernandez, María Luisa Marina, Leonor Nozal, Antonio L. CregoAbstract:A novel screening method using an automated flow injection electrospray ionization tandem mass spectrometry system is proposed for the simultaneous determination of five Nonprotein Amino Acids (β-alanine, alloisoleucine, ornithine, citrulline, pyroglutamic acid) and three betaines (glycine betaine, trigonelline, proline betaine) after derivatization with butanolic HCl. MS/MS experiments were carried out in a triple-quadrupole instrument using multiple reaction monitoring mode in <2 min. The proposed method provided high fingerprinting power to identify the presence of five of the studied compounds in different types of vegetable oils (soybean, sunflower, corn, olive) with LODs at parts per billion levels. The method was validated, and different mixtures of extra virgin olive oil with seed oils were analyzed, achieving the typification for the detection of adulterations in extra virgin olive oils up to 2% w/w. The Nonprotein Amino acid ornithine was confirmed as a marker for adulteration in the olive oils ...
María Luisa Marina - One of the best experts on this subject based on the ideXlab platform.
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Advances in the Determination of Nonprotein Amino Acids in Foods and Biological Samples by Capillary Electrophoresis.
Critical reviews in analytical chemistry, 2019Co-Authors: Raquel Pérez-míguez, Sandra Salido-fortuna, María Castro-puyana, María Luisa MarinaAbstract:There are hundreds of Nonprotein Amino Acids whose importance in food and biological matrices is still unknown. Many of these compounds can be found in food as products formed during the processing...
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Potential of vancomycin for the enantiomeric resolution of FMOC‐Amino Acids by capillary electrophoresis‐ion‐trap‐mass spectrometry
Electrophoresis, 2014Co-Authors: Laura Sánchez-hernández, María Castro-puyana, María Luisa Marina, Elena Domínguez-vega, Cristina Montealegre, Antonio L. CregoAbstract:The potential of the antibiotic vancomycin (VC) as chiral selector for the enantiomeric separation of Amino Acids by CE-ESI-MS/MS² was investigated for the first time in this work. Derivatization of Amino Acids with FMOC-Cl was carried out to enable their interaction with VC as well as the formation of precursor ions with larger m/z which were employed in MS² experiments. The partial filling of a coated capillary was employed to avoid the loss in MS sensitivity originated by the introduction of VC in the ionization source. Under optimized conditions, the simultaneous enantiomeric separation and unequivocal identification of 17 Amino Acids (two of them being Nonprotein Amino Acids) took place in about 20 min with LODs in the micromolar range.
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Determination of Nonprotein Amino Acids and betaines in vegetable oils by flow injection triple-quadrupole tandem mass spectrometry: a screening method for the detection of adulterations of olive oils.
Journal of agricultural and food chemistry, 2012Co-Authors: Laura Sánchez-hernández, María Luisa Marina, Leonor Nozal, Antonio L. CregoAbstract:A novel screening method using an automated flow injection electrospray ionization tandem mass spectrometry system is proposed for the simultaneous determination of five Nonprotein Amino Acids (β-alanine, alloisoleucine, ornithine, citrulline, pyroglutamic acid) and three betaines (glycine betaine, trigonelline, proline betaine) after derivatization with butanolic HCl. MS/MS experiments were carried out in a triple-quadrupole instrument using multiple reaction monitoring mode in
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determination of Nonprotein Amino Acids and betaines in vegetable oils by flow injection triple quadrupole tandem mass spectrometry a screening method for the detection of adulterations of olive oils
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Laura Sanchezhernandez, María Luisa Marina, Leonor Nozal, Antonio L. CregoAbstract:A novel screening method using an automated flow injection electrospray ionization tandem mass spectrometry system is proposed for the simultaneous determination of five Nonprotein Amino Acids (β-alanine, alloisoleucine, ornithine, citrulline, pyroglutamic acid) and three betaines (glycine betaine, trigonelline, proline betaine) after derivatization with butanolic HCl. MS/MS experiments were carried out in a triple-quadrupole instrument using multiple reaction monitoring mode in <2 min. The proposed method provided high fingerprinting power to identify the presence of five of the studied compounds in different types of vegetable oils (soybean, sunflower, corn, olive) with LODs at parts per billion levels. The method was validated, and different mixtures of extra virgin olive oil with seed oils were analyzed, achieving the typification for the detection of adulterations in extra virgin olive oils up to 2% w/w. The Nonprotein Amino acid ornithine was confirmed as a marker for adulteration in the olive oils ...
Laura Sánchez-hernández - One of the best experts on this subject based on the ideXlab platform.
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Potential of vancomycin for the enantiomeric resolution of FMOC‐Amino Acids by capillary electrophoresis‐ion‐trap‐mass spectrometry
Electrophoresis, 2014Co-Authors: Laura Sánchez-hernández, María Castro-puyana, María Luisa Marina, Elena Domínguez-vega, Cristina Montealegre, Antonio L. CregoAbstract:The potential of the antibiotic vancomycin (VC) as chiral selector for the enantiomeric separation of Amino Acids by CE-ESI-MS/MS² was investigated for the first time in this work. Derivatization of Amino Acids with FMOC-Cl was carried out to enable their interaction with VC as well as the formation of precursor ions with larger m/z which were employed in MS² experiments. The partial filling of a coated capillary was employed to avoid the loss in MS sensitivity originated by the introduction of VC in the ionization source. Under optimized conditions, the simultaneous enantiomeric separation and unequivocal identification of 17 Amino Acids (two of them being Nonprotein Amino Acids) took place in about 20 min with LODs in the micromolar range.
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Determination of Nonprotein Amino Acids and betaines in vegetable oils by flow injection triple-quadrupole tandem mass spectrometry: a screening method for the detection of adulterations of olive oils.
Journal of agricultural and food chemistry, 2012Co-Authors: Laura Sánchez-hernández, María Luisa Marina, Leonor Nozal, Antonio L. CregoAbstract:A novel screening method using an automated flow injection electrospray ionization tandem mass spectrometry system is proposed for the simultaneous determination of five Nonprotein Amino Acids (β-alanine, alloisoleucine, ornithine, citrulline, pyroglutamic acid) and three betaines (glycine betaine, trigonelline, proline betaine) after derivatization with butanolic HCl. MS/MS experiments were carried out in a triple-quadrupole instrument using multiple reaction monitoring mode in
Fernand Lambein - One of the best experts on this subject based on the ideXlab platform.
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Nonprotein Amino Acids in edible lentil and garden pea seedlings.
Amino acids, 2001Co-Authors: Pascale Rozan, Yu-haey Kuo, Fernand LambeinAbstract:Commercial edible seedlings of garden pea (Pisum sativum L.) and lentil (Lens culinaris L.) contain high concentration of Nonprotein Amino Acids and trigonelline. Both seedlings grown in the laboratory or purchased in a supermarket were studied by HPLC. Samples from both origins contained trigonelline, alpha-Aminoadipic acid, homoserine, beta-(isoxazolin-5-on-2-yl)-alanine (BIA), and gamma-glutamyl-BIA. Garden pea seedlings also contained a uracil-alanine derivative (isowillardiine) in substantial amount. Some of these compounds such as BIA and alpha-Aminoadipic acid have neurotoxic activity.
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Nonprotein Amino Acids in edible lentil and garden pea seedlings
Amino Acids, 2001Co-Authors: Pascale Rozan, Yu-haey Kuo, Fernand LambeinAbstract:Commercial edible seedlings of garden pea (Pisum sativum L.) and lentil (Lens culinaris L.) contain high concentration of Nonprotein Amino Acids and trigonelline. Both seedlings grown in the laboratory or purchased in a supermarket were studied by HPLC. Samples from both origins contained trigonelline, α-Aminoadipic acid, homoserine, β-(isoxazolin-5-on-2-yl)-alanine (BIA), and γ-glutamyl-BIA. Garden pea seedlings also contained a uracil-alanine derivative (isowillardiine) in substantial amount. Some of these compounds such as BIA and α-Aminoadipic acid have neurotoxic activity.
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Amino Acids in seeds and seedlings of the genus Lens.
Phytochemistry, 2001Co-Authors: Pascale Rozan, Yu-haey Kuo, Fernand LambeinAbstract:The Amino acid content of seeds and 4-day-old seedlings were studied in five species of lentil: Lens culinaris, L. orientalis, L. ervoides, L. nigricans and L. odemensis. Free Amino acid and also total protein Amino acid content after HCl hydrolysis were determined by HPLC. The Nonprotein UV-absorbing Amino Acids were determined by capillary zone electrophoresis (CZE). The content of free protein Amino Acids in seeds varied among species and increased dramatically after germination. Asparagine is quantitatively most important in both seed and seedling. The content of free Nonprotein Amino Acids is variable in seeds and seedlings. gamma-Hydroxyarginine, gamma-hydroxyornithine, alpha-Aminobutyric acid and taurine were found in both seeds and seedlings. Homoarginine was found in four species but not in L. orientalis while gamma-Aminobutyric acid (GABA), alpha-Aminoadipic acid (alpha-aaa) and three isoxazolinone derivatives: beta-(isoxazolin-5-on-2-yl)-alanine (BIA), gamma-glutamyl-BIA (gamma-glu-BIA) and 2-carboxymethyl-isoxazolin-5-one (CMI) were found exclusively in the seedlings. CMI was identified for the first time in lentil species. Lathyrine, beta-(2-Amino-pyrimidine-4-yl)-alanine, which was reported to be in the seeds of some Lathyrus species was confirmed to be present also in the seedling of L. culinaris (trace amount), L. nigricans and L. odemensis. Trigonelline (N-methyl-nicotinic acid), a plant hormone, is present both in seeds and seedlings in different concentrations except in L. ervoides. The different combination of Nonprotein Amino Acids among the species gives indication of their genetic relationship and might partly explain the varying compatibility for interspecies crossing.
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Free Amino Acids present in commercially available seedlings sold for human consumption. A potential hazard for consumers.
Journal of agricultural and food chemistry, 2000Co-Authors: Pascale Rozan, Yu-haey Kuo, Fernand LambeinAbstract:The importance of fresh seedlings for human consumption on European markets continues to increase. Although the contents of free Amino Acids and potentially toxic free Nonprotein Amino Acids in these fresh and supposedly healthy seedlings is very different from those of the seeds, the crude composition is never mentioned on commercial packages. A commercial product containing seven different kinds of fresh seedlings including kamut, adzuki bean, chickpea, mungbean, pinto bean, garden pea, and lentil has been analyzed by HPLC. Per 100 g of fresh product, 548.2 mg of total free Amino Acids was found, of which 56.7 mg is free Nonprotein Amino Acids including beta-(isoxazolin-5-on-2-yl)alanine, homoserine, and isowillardiine and the plant hormone trigonelline (N-methylnicotinic acid). The highest amounts of free Nonprotein Amino Acids and trigonelline are found in garden pea (28.3 mg/100 g), mungbean (9.59 mg/100 g), and lentil (7.50 mg/100 g) seedlings. Trigonelline is present in all legume seedlings examined.
Leonor Nozal - One of the best experts on this subject based on the ideXlab platform.
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Determination of Nonprotein Amino Acids and betaines in vegetable oils by flow injection triple-quadrupole tandem mass spectrometry: a screening method for the detection of adulterations of olive oils.
Journal of agricultural and food chemistry, 2012Co-Authors: Laura Sánchez-hernández, María Luisa Marina, Leonor Nozal, Antonio L. CregoAbstract:A novel screening method using an automated flow injection electrospray ionization tandem mass spectrometry system is proposed for the simultaneous determination of five Nonprotein Amino Acids (β-alanine, alloisoleucine, ornithine, citrulline, pyroglutamic acid) and three betaines (glycine betaine, trigonelline, proline betaine) after derivatization with butanolic HCl. MS/MS experiments were carried out in a triple-quadrupole instrument using multiple reaction monitoring mode in
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determination of Nonprotein Amino Acids and betaines in vegetable oils by flow injection triple quadrupole tandem mass spectrometry a screening method for the detection of adulterations of olive oils
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Laura Sanchezhernandez, María Luisa Marina, Leonor Nozal, Antonio L. CregoAbstract:A novel screening method using an automated flow injection electrospray ionization tandem mass spectrometry system is proposed for the simultaneous determination of five Nonprotein Amino Acids (β-alanine, alloisoleucine, ornithine, citrulline, pyroglutamic acid) and three betaines (glycine betaine, trigonelline, proline betaine) after derivatization with butanolic HCl. MS/MS experiments were carried out in a triple-quadrupole instrument using multiple reaction monitoring mode in <2 min. The proposed method provided high fingerprinting power to identify the presence of five of the studied compounds in different types of vegetable oils (soybean, sunflower, corn, olive) with LODs at parts per billion levels. The method was validated, and different mixtures of extra virgin olive oil with seed oils were analyzed, achieving the typification for the detection of adulterations in extra virgin olive oils up to 2% w/w. The Nonprotein Amino acid ornithine was confirmed as a marker for adulteration in the olive oils ...
