The Experts below are selected from a list of 25563 Experts worldwide ranked by ideXlab platform
David S Perlin - One of the best experts on this subject based on the ideXlab platform.
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detection of aspergillus fumigatus in a rat model of invasive pulmonary aspergillosis by real time Nucleic Acid Sequence based amplification
Journal of Clinical Microbiology, 2010Co-Authors: Yanan Zhao, Steven Park, Peter Warn, Raghdaa Shrief, Elizabeth Harrison, David S PerlinAbstract:Rapid and sensitive detection of Aspergillus from clinical samples may facilitate the early diagnosis of invasive pulmonary aspergillosis (IPA). A real-time Nucleic Acid Sequence-based amplification (NASBA) method was investigated by use of an inhalational rat model of IPA. Immunosuppressed male Sprague-Dawley rats were exposed to Aspergillus fumigatus spores for an hour in an aerosol chamber. Bronchoalveolar lavage (BAL) fluid, lung tissues, and whole blood were collected from five infected rats at 1, 24, 48, 72, and 96 h postinfection and five uninfected rats at the end of the experiment. Total Nucleic Acid (TNA) was extracted on an easyMAG instrument. A primer-molecular beacon set targeting 28S rRNA was designed to detect Aspergillus spp. The results were compared to those of quantitative PCR (qPCR) (18S rDNA) and quantitative culture. The analytical sensitivity of the real-time NASBA assay was <1 CFU/assay. A linear range of detection was demonstrated over 5 log units of conidia (10 to 10(5) spores). Both NASBA and qPCR showed a progressive increase in lung tissue burdens, while the CFU counts were stable over time. The fungal burdens in BAL fluid were more variable and not indicative of a progressive infection. The results of both real-time assays correlated well for both sample types (r = 0.869 and P < 0.0001 for lung tissue, r = 0.887 and P < 0.0001 for BAL fluid). For all whole-blood specimens, NASBA identified Aspergillus-positive samples in the group from which samples were collected at 72 h postinfection (three of five samples) and the group from which samples were collected at 96 h postinfection (five of five samples), but no positive results were obtained by culture or PCR. Real-time NASBA is highly sensitive and useful for the detection of Aspergillus in an experimental model of IPA.
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rapid real time Nucleic Acid Sequence based amplification molecular beacon platform to detect fungal and bacterial bloodstream infections
Journal of Clinical Microbiology, 2009Co-Authors: Yanan Zhao, Steven Park, Barry N Kreiswirth, Christine C Ginocchio, Raphael Veyret, Ali Laayoun, Alain Troesch, David S PerlinAbstract:Bloodstream infections (BSIs) are a significant cause of morbidity and mortality. Successful patient outcomes are diminished by a failure to rapidly diagnose these infections and initiate appropriate therapy. A rapid and reliable diagnostic platform of high sensitivity is needed for the management of patients with BSIs. The combination of an RNA-dependent Nucleic Acid Sequence-based amplification and molecular beacon (NASBA-MB) detection system in multiplex format was developed to rapidly detect medically important BSI organisms. Probes and primers representing pan-gram-negative, pan-gram-positive, pan-fungal, pan-Candida, and pan-Aspergillus organisms were established utilizing 16S and 28S rRNA targets for bacteria and fungi, respectively. Two multiplex panels were developed to rapidly discriminate bacterial or fungal infections at the subkingdom/genus level with a sensitivity of 1 to 50 genomes. A clinical study was performed to evaluate the accuracy of this platform by evaluating 570 clinical samples from a tertiary-care hospital group using blood bottle samples. The sensitivity, specificity, and Youden's index values for pan-gram-positive detection and pan-gram-negative detection were 99.7%, 100%, 0.997 and 98.6%, 95.9%, 0.945, respectively. The positive predictive values (PPV) and the negative predictive values (NPV) for these two probes were 100, 90.7, and 99.4, 99.4, respectively. Pan-fungal and pan-Candida probes showed 100% sensitivity, specificity, PPV, and NPV, and the pan-Aspergillus probe showed 100% NPV. Robust signals were observed for all probes in the multiplex panels, with signal detection in <15 min. The multiplex real-time NASBA-MB assay provides a valuable platform for the rapid and specific diagnosis of bloodstream pathogens, and reliable pathogen identification and characterization can be obtained in under 3 h.
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rapid real time Nucleic Acid Sequence based amplification molecular beacon platform to detect fungal and bacterial bloodstream infections
Journal of Clinical Microbiology, 2009Co-Authors: Yanan Zhao, Steven Park, Barry N Kreiswirth, Christine C Ginocchio, Raphael Veyret, Ali Laayoun, Alain Troesch, David S PerlinAbstract:Bloodstream infections (BSIs) are a significant cause of morbidity and mortality. Successful patient outcomes are diminished by a failure to rapidly diagnose these infections and initiate appropriate therapy. A rapid and reliable diagnostic platform of high sensitivity is needed for the management of patients with BSIs. The combination of an RNA-dependent Nucleic Acid Sequence-based amplification and molecular beacon (NASBAMB) detection system in multiplex format was developed to rapidly detect medically important BSI organisms. Probes and primers representing pan-gram-negative, pan-gram-positive, pan-fungal, pan-Candida, and panAspergillus organisms were established utilizing 16S and 28S rRNA targets for bacteria and fungi, respectively. Two multiplex panels were developed to rapidly discriminate bacterial or fungal infections at the subkingdom/ genus level with a sensitivity of 1 to 50 genomes. A clinical study was performed to evaluate the accuracy of this platform by evaluating 570 clinical samples from a tertiary-care hospital group using blood bottle samples. The sensitivity, specificity, and Youden’s index values for pan-gram-positive detection and pan-gram-negative detection were 99.7%, 100%, 0.997 and 98.6%, 95.9%, 0.945, respectively. The positive predictive values (PPV) and the negative predictive values (NPV) for these two probes were 100, 90.7, and 99.4, 99.4, respectively. Pan-fungal and pan-Candida probes showed 100% sensitivity, specificity, PPV, and NPV, and the pan-Aspergillus probe showed 100% NPV. Robust signals were observed for all probes in the multiplex panels, with signal detection in <15 min. The multiplex real-time NASBA-MB assay provides a valuable platform for the rapid and specific diagnosis of bloodstream pathogens, and reliable pathogen identification and characterization can be obtained in under 3 h. Bloodstream infections (BSIs) are a significant cause of morbidity and mortality in the United States. Approximately 250,000 nosocomial BSIs occur annually, with a crude mortality rate of 20 to 50% (2, 17, 38). Based on data from death certificates, BSIs are the 10th leading cause of death in the United States (33), and the age-adjusted death rate has risen by 78% during the past two decades (10). The mortality rates of BSI patients are increased with measurable delays in the institution of effective, appropriate antimicrobial therapy (19, 35). Inadequate therapy was an independent determinant of mortality for individual organisms such as Candida species (21). Yu et al., in a U.S. multicenter study, found that failure to start antimicrobial therapy within 24 h of illness onset was strongly correlated with a higher probability of a 28-day mortality (49). A rapid and reliable diagnosis may be the optimal method for avoiding such delays in the treatment of BSIs. A variety of microorganisms can cause BSIs. A nationwide surveillance study showed that gram-positive bacteria, gramnegative bacteria, and fungi (including yeasts and molds) caused 65, 25, and 9.5% of monomicrobial BSIs, respectively. The most common organisms associated with BSIs are the coagulase-negative staphylococci (CoNS), Staphylococcus aureus, Enterococcus spp., and Candida spp. and the gram-negative bacilli Escherichia coli and Klebsiella spp.(47). The conventional diagnostic test for BSI is blood culture, which usually is
Yi Wang - One of the best experts on this subject based on the ideXlab platform.
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visual and multiplex detection of Nucleic Acid Sequence by multiple cross displacement amplification coupled with gold nanoparticle based lateral flow biosensor
Sensors and Actuators B-chemical, 2017Co-Authors: Yi Wang, Yan Wang, Lu ZhangAbstract:Abstract Here, we reported on the establishment of a nearly instrument free, simple and rapid molecular technique, which incorporated multiple cross displacement amplification coupled with lateral flow biosensor (MCDA-LFB) for the visual, sensitive and specific detection of Nucleic Acid Sequence. The MCDA-LFB assay was able to simultaneously detect and correctly differentiate multiple targets using a 40 min amplification reaction followed by a 2 min incubation of the products on the visualization biosensor. The biosensor was devised to detect three targets, a chromatography control, the MCDA target amplicon I and II, and the interpretation of test results is based on the appearance of red lines on the reaction pad. As a proof of concept, Shigella and Salmonella strains were detected by MCDA-LFB technique to demonstrate the availability of target analysis. The analytical sensitivity, specificity and practical application of MCDA-LFB assay were successfully evaluated in pure culture and blood samples, and the whole procedure, including specimen (blood sample) processing (35 min), isothermal reaction (40 min), and result reporting (5 min), was completed within 80 min. Therefore, the simplicity, rapidity and nearly apparatus-free platform of the MCDA-LFB assay make it practical for point-of-care testing, field detection, ‘on-site’ diagnosis and more.
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rapid and sensitive isothermal detection of Nucleic Acid Sequence by multiple cross displacement amplification
Scientific Reports, 2015Co-Authors: Yi Wang, Yan Wang, Lijuan Luo, Dongxin Liu, Dong Jin, Kai LiuAbstract:Rapid and Sensitive Isothermal Detection of Nucleic-Acid Sequence by Multiple Cross Displacement Amplification
Yanan Zhao - One of the best experts on this subject based on the ideXlab platform.
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detection of aspergillus fumigatus in a rat model of invasive pulmonary aspergillosis by real time Nucleic Acid Sequence based amplification
Journal of Clinical Microbiology, 2010Co-Authors: Yanan Zhao, Steven Park, Peter Warn, Raghdaa Shrief, Elizabeth Harrison, David S PerlinAbstract:Rapid and sensitive detection of Aspergillus from clinical samples may facilitate the early diagnosis of invasive pulmonary aspergillosis (IPA). A real-time Nucleic Acid Sequence-based amplification (NASBA) method was investigated by use of an inhalational rat model of IPA. Immunosuppressed male Sprague-Dawley rats were exposed to Aspergillus fumigatus spores for an hour in an aerosol chamber. Bronchoalveolar lavage (BAL) fluid, lung tissues, and whole blood were collected from five infected rats at 1, 24, 48, 72, and 96 h postinfection and five uninfected rats at the end of the experiment. Total Nucleic Acid (TNA) was extracted on an easyMAG instrument. A primer-molecular beacon set targeting 28S rRNA was designed to detect Aspergillus spp. The results were compared to those of quantitative PCR (qPCR) (18S rDNA) and quantitative culture. The analytical sensitivity of the real-time NASBA assay was <1 CFU/assay. A linear range of detection was demonstrated over 5 log units of conidia (10 to 10(5) spores). Both NASBA and qPCR showed a progressive increase in lung tissue burdens, while the CFU counts were stable over time. The fungal burdens in BAL fluid were more variable and not indicative of a progressive infection. The results of both real-time assays correlated well for both sample types (r = 0.869 and P < 0.0001 for lung tissue, r = 0.887 and P < 0.0001 for BAL fluid). For all whole-blood specimens, NASBA identified Aspergillus-positive samples in the group from which samples were collected at 72 h postinfection (three of five samples) and the group from which samples were collected at 96 h postinfection (five of five samples), but no positive results were obtained by culture or PCR. Real-time NASBA is highly sensitive and useful for the detection of Aspergillus in an experimental model of IPA.
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rapid real time Nucleic Acid Sequence based amplification molecular beacon platform to detect fungal and bacterial bloodstream infections
Journal of Clinical Microbiology, 2009Co-Authors: Yanan Zhao, Steven Park, Barry N Kreiswirth, Christine C Ginocchio, Raphael Veyret, Ali Laayoun, Alain Troesch, David S PerlinAbstract:Bloodstream infections (BSIs) are a significant cause of morbidity and mortality. Successful patient outcomes are diminished by a failure to rapidly diagnose these infections and initiate appropriate therapy. A rapid and reliable diagnostic platform of high sensitivity is needed for the management of patients with BSIs. The combination of an RNA-dependent Nucleic Acid Sequence-based amplification and molecular beacon (NASBA-MB) detection system in multiplex format was developed to rapidly detect medically important BSI organisms. Probes and primers representing pan-gram-negative, pan-gram-positive, pan-fungal, pan-Candida, and pan-Aspergillus organisms were established utilizing 16S and 28S rRNA targets for bacteria and fungi, respectively. Two multiplex panels were developed to rapidly discriminate bacterial or fungal infections at the subkingdom/genus level with a sensitivity of 1 to 50 genomes. A clinical study was performed to evaluate the accuracy of this platform by evaluating 570 clinical samples from a tertiary-care hospital group using blood bottle samples. The sensitivity, specificity, and Youden's index values for pan-gram-positive detection and pan-gram-negative detection were 99.7%, 100%, 0.997 and 98.6%, 95.9%, 0.945, respectively. The positive predictive values (PPV) and the negative predictive values (NPV) for these two probes were 100, 90.7, and 99.4, 99.4, respectively. Pan-fungal and pan-Candida probes showed 100% sensitivity, specificity, PPV, and NPV, and the pan-Aspergillus probe showed 100% NPV. Robust signals were observed for all probes in the multiplex panels, with signal detection in <15 min. The multiplex real-time NASBA-MB assay provides a valuable platform for the rapid and specific diagnosis of bloodstream pathogens, and reliable pathogen identification and characterization can be obtained in under 3 h.
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rapid real time Nucleic Acid Sequence based amplification molecular beacon platform to detect fungal and bacterial bloodstream infections
Journal of Clinical Microbiology, 2009Co-Authors: Yanan Zhao, Steven Park, Barry N Kreiswirth, Christine C Ginocchio, Raphael Veyret, Ali Laayoun, Alain Troesch, David S PerlinAbstract:Bloodstream infections (BSIs) are a significant cause of morbidity and mortality. Successful patient outcomes are diminished by a failure to rapidly diagnose these infections and initiate appropriate therapy. A rapid and reliable diagnostic platform of high sensitivity is needed for the management of patients with BSIs. The combination of an RNA-dependent Nucleic Acid Sequence-based amplification and molecular beacon (NASBAMB) detection system in multiplex format was developed to rapidly detect medically important BSI organisms. Probes and primers representing pan-gram-negative, pan-gram-positive, pan-fungal, pan-Candida, and panAspergillus organisms were established utilizing 16S and 28S rRNA targets for bacteria and fungi, respectively. Two multiplex panels were developed to rapidly discriminate bacterial or fungal infections at the subkingdom/ genus level with a sensitivity of 1 to 50 genomes. A clinical study was performed to evaluate the accuracy of this platform by evaluating 570 clinical samples from a tertiary-care hospital group using blood bottle samples. The sensitivity, specificity, and Youden’s index values for pan-gram-positive detection and pan-gram-negative detection were 99.7%, 100%, 0.997 and 98.6%, 95.9%, 0.945, respectively. The positive predictive values (PPV) and the negative predictive values (NPV) for these two probes were 100, 90.7, and 99.4, 99.4, respectively. Pan-fungal and pan-Candida probes showed 100% sensitivity, specificity, PPV, and NPV, and the pan-Aspergillus probe showed 100% NPV. Robust signals were observed for all probes in the multiplex panels, with signal detection in <15 min. The multiplex real-time NASBA-MB assay provides a valuable platform for the rapid and specific diagnosis of bloodstream pathogens, and reliable pathogen identification and characterization can be obtained in under 3 h. Bloodstream infections (BSIs) are a significant cause of morbidity and mortality in the United States. Approximately 250,000 nosocomial BSIs occur annually, with a crude mortality rate of 20 to 50% (2, 17, 38). Based on data from death certificates, BSIs are the 10th leading cause of death in the United States (33), and the age-adjusted death rate has risen by 78% during the past two decades (10). The mortality rates of BSI patients are increased with measurable delays in the institution of effective, appropriate antimicrobial therapy (19, 35). Inadequate therapy was an independent determinant of mortality for individual organisms such as Candida species (21). Yu et al., in a U.S. multicenter study, found that failure to start antimicrobial therapy within 24 h of illness onset was strongly correlated with a higher probability of a 28-day mortality (49). A rapid and reliable diagnosis may be the optimal method for avoiding such delays in the treatment of BSIs. A variety of microorganisms can cause BSIs. A nationwide surveillance study showed that gram-positive bacteria, gramnegative bacteria, and fungi (including yeasts and molds) caused 65, 25, and 9.5% of monomicrobial BSIs, respectively. The most common organisms associated with BSIs are the coagulase-negative staphylococci (CoNS), Staphylococcus aureus, Enterococcus spp., and Candida spp. and the gram-negative bacilli Escherichia coli and Klebsiella spp.(47). The conventional diagnostic test for BSI is blood culture, which usually is
Henk D. F. H. Schallig - One of the best experts on this subject based on the ideXlab platform.
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comparison between quantitative Nucleic Acid Sequence based amplification real time reverse transcriptase pcr and real time pcr for quantification of leishmania parasites
Journal of Clinical Microbiology, 2008Co-Authors: Wendy F. Van Der Meide, Gerard J. Schoone, William R. Faber, Leíla I. A. R. C. Coelho, Jorge Augusto De Oliveira Guerra, Marit Farenhorst, Inge Peekel, Henk D. F. H. SchalligAbstract:DNA or RNA amplification methods for detection of Leishmania parasites have advantages regarding sensitivity and potential quantitative characteristics in comparison with conventional diagnostic methods but are often still not routinely applied. However, the use and application of molecular assays are increasing, but comparative studies on the performance of these different assays are lacking. The aim of this study was to compare three molecular assays for detection and quantification of Leishmania parasites in serial dilutions of parasites and in skin biopsies collected from cutaneous leishmaniasis (CL) patients in Manaus, Brazil. A serial dilution of promastigotes spiked in blood was tested in triplicate in three different runs by quantitative Nucleic Acid Sequence-based amplification (QT-NASBA), quantitative real-time reverse transcriptase PCR (qRT-PCR), and quantitative real-time PCR (qPCR). In addition, the costs, durations, and numbers of handling steps were compared, and 84 skin biopsies from patients with suspected CL were tested. Both QT-NASBA and qRT-PCR had a detection limit of 100 parasites/ml of blood, while qPCR detected 1,000 parasites/ml. QT-NASBA had the lowest range of intra-assay variation (coefficients of variation [CV], 0.5% to 3.3%), while qPCR had the lowest range of interassay variation (CV, 0.4% to 5.3%). Furthermore, qRT-PCR had higher r2 values and amplification efficiencies than qPCR, and qPCR and qRT-PCR had faster procedures than QT-NASBA. All assays performed equally well with patient samples, with significant correlations between parasite counts. Overall, qRT-PCR is preferred over QT-NASBA and qPCR as the most optimal diagnostic assay for quantification of Leishmania parasites, since it was highly sensitive and reproducible and the procedure was relatively fast.
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detection and identification of human plasmodium species with real time quantitative Nucleic Acid Sequence based amplification
Malaria Journal, 2006Co-Authors: Petra F Mens, Gerard J. Schoone, Piet A Kager, Henk D. F. H. SchalligAbstract:Background Decisions concerning malaria treatment depend on species identification causing disease. Microscopy is most frequently used, but at low parasitaemia (<20 parasites/μl) the technique becomes less sensitive and time consuming. Rapid diagnostic tests based on Plasmodium antigen detection do often not allow for species discrimination as microscopy does, but also become insensitive at <100 parasites/μl.
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Quantitative Nucleic Acid Sequence-Based Assay as a New Molecular Tool for Detection and Quantification of Leishmania Parasites in Skin Biopsy Samples
Journal of clinical microbiology, 2005Co-Authors: Wendy F. Van Der Meide, Gerard J. Schoone, William R. Faber, J. E. Zeegelaar, Henry J. C. De Vries, Yusuf Özbel, Rudy F. M. Lai A Fat, Leíla I. A. R. C. Coelho, Masoom Kassi, Henk D. F. H. SchalligAbstract:Currently available methods for the diagnosis of cutaneous leishmaniasis (CL) have low sensitivities or are unable to quantify the number of viable parasites. This constitutes a major obstacle for the diagnosis of the disease and for the study of the effectiveness of treatment schedules and urges the development of improved detection methods. In this study, quantitative Nucleic Acid Sequence-based amplification (QT-NASBA) technology was used to detect and quantify Leishmania parasites in skin biopsy samples from CL patients. The assay is based on the detection of a small subunit rRNA (18S rRNA), which may allow for the detection of viable parasites. The QT-NASBA assay was evaluated using in vitro-cultured promastigotes and amastigotes and 2-mm skin biopsy samples from Old and New World CL patients. The study demonstrated that the lower detection limit of the QT-NASBA was two parasites per biopsy sample. Parasites could be quantified in a range of 2 to 11,300,000 parasites per biopsy sample. The QT-NASBA could detect levels of parasites 100-fold lower than those detected by conventional PCR. Test evaluation revealed that the QT-NASBA had a sensitivity of 97.5% and a specificity of 100% in the present study. The QT-NASBA is a highly sensitive and specific method that allows quantification of both Old and New World Leishmania parasites in skin biopsy samples and may provide an important tool for diagnosis as well as for monitoring the therapy of CL patients.
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real time Nucleic Acid Sequence based amplification is more convenient than real time pcr for quantification of plasmodium falciparum
Journal of Clinical Microbiology, 2005Co-Authors: Petra Schneider, Gerard J. Schoone, Henk D. F. H. Schallig, Liselotte Wolters, P Sillekens, Rob Hermsen, Robert W SauerweinAbstract:Determination of the number of malaria parasites by routine or even expert microscopy is not always sufficiently sensitive for detailed quantitative studies on the population dynamics of Plasmodium falciparum, such as intervention or vaccine trials. To circumvent this problem, two more sensitive assays, real-time quantitative Nucleic Acid Sequence-based amplification (QT-NASBA) and real-time quantitative PCR (QT-PCR) were compared for quantification of P. falciparum parasites. QT-NASBA was adapted to molecular beacon real-time detection technology, which enables a reduction of the time of analysis and of contamination risk while retaining the specificity and sensitivity of the original assay. Both QT-NASBA and QT-PCR have a sensitivity of 20 parasites/ml of blood, but QT-PCR requires a complicated DNA extraction procedure and the use of 500 μl of venous blood to achieve this sensitivity, compared to 50 μl of finger prick blood for real-time QT-NASBA. Both techniques show a significant correlation to microscopic parasite counts, and the quantification results of the two real-time assays are significantly correlated for in vitro as well as in vivo samples. However, in comparison to real-time QT-PCR, the results of real-time QT-NASBA can be obtained 12 h earlier, with relatively easy RNA extraction and use of finger prick blood samples. The prospective development of multiplex QT-NASBA for detection of various P. falciparum developmental stages increases the value of QT-NASBA for malaria studies. Therefore, for studies requiring sensitive and accurate detection of P. falciparum parasites in large numbers of samples, the use of real-time QT-NASBA is preferred over that of real-time QT-PCR.
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quantification of plasmodium falciparum gametocytes in differential stages of development by quantitative Nucleic Acid Sequence based amplification
Molecular and Biochemical Parasitology, 2004Co-Authors: Petra Schneider, Gerard J. Schoone, Henk D. F. H. Schallig, Danielle F Verhage, D S C Telgt, W M C Eling, Robert W SauerweinAbstract:Two quantitative Nucleic Acid Sequence-based amplification assays (QT-NASBA) based on Pfs16 and Pfs25, have been developed to quantify sexual stage commitment and mature gametocytes of Plasmodium falciparum. Pfs16 mRNA is expressed in all sexual forms including sexually committed ring stages while expression of Pfs25 mRNA is restricted to late stage gametocytes. Both assays showed a sensitivity of one sexual stage parasite/microl of blood. Blood samples from experimentally infected non-immune human volunteers were tested for Plasmodium falciparum by standard microscopy, a previously developed asexual 18S rRNA QT-NASBA, Pfs16 and Pfs25 mRNA QT-NASBA. Pfs16 QT-NASBA was positive in 9 out of 10 volunteers within 48 h after first detection of 18S rRNA, mostly before or at the day of positive microscopy. In contrast, the Pfs25 mRNA QT-NASBA was negative during the 28 days of follow-up, but consistently positive in gametocyte samples from naturally infected Kenyan patients. These data suggest that sexual stage commitment can occur early in the blood-stage infection without successful maturation into infectious gametocytes. In conclusion, Pfs16 and Pfs25 QT-NASBA assays in combination with a previously developed asexual stage QT-NASBA allow for the separate quantification of all developmental stages present in the circulation. The application of sexual stage QT-NASBA assays may contribute to a better understanding of the biology and epidemiology of malaria transmission.
Kai Liu - One of the best experts on this subject based on the ideXlab platform.
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rapid and sensitive isothermal detection of Nucleic Acid Sequence by multiple cross displacement amplification
Scientific Reports, 2015Co-Authors: Yi Wang, Yan Wang, Lijuan Luo, Dongxin Liu, Dong Jin, Kai LiuAbstract:Rapid and Sensitive Isothermal Detection of Nucleic-Acid Sequence by Multiple Cross Displacement Amplification
