The Experts below are selected from a list of 204 Experts worldwide ranked by ideXlab platform
Richard R. Miselis - One of the best experts on this subject based on the ideXlab platform.
-
Thyrotropin‐releasing hormone‐immunoreactive projections to the dorsal motor Nucleus and the Nucleus of the solitary tract of the rat
The Journal of Comparative Neurology, 1991Co-Authors: Richard B Lynn, Margaret S. Kreider, Richard R. MiselisAbstract:: Thyrotropin-releasing hormone-immunoreactive nerve terminals heavily innervate the dorsal motor Nucleus and Nucleus of the solitary tract, whereas cell bodies containing thyrotropin-releasing hormone residue most densely in the hypothalamus and Raphe nuclei. By using double-labeling techniques accomplished by retrograde transport of Fluoro-Gold following microinjection into the dorsal motor Nucleus/Nucleus of the solitary tract combined with immunohistochemistry for thyrotropin-releasing hormone, it was demonstrated that thyrotropin-releasing hormone-immunoreactive neurons projecting to the dorsal motor Nucleus/Nucleus of the solitary tract reside in the Nucleus Raphe Pallidus, Nucleus Raphe obscurus, and the parapyramidal region of the ventral medulla, but not in the paraventricular Nucleus of the hypothalamus. The parapyramidal region includes an area along the ventral surface of the caudal medulla, lateral to the pyramidal tract and inferior olivary Nucleus and ventromedial to the lateral reticular Nucleus. Varying the position of the Fluoro-Gold injection site revealed a rostral to caudal topographic organization of these Raphe and parapyramidal projections.
David E Millhorn - One of the best experts on this subject based on the ideXlab platform.
-
Hypoxia and electrical stimulation of the carotid sinus nerve induce fos‐like immunoreactivity within catecholaminergic and serotoninergic neurons of the rat brainstem
The Journal of comparative neurology, 1994Co-Authors: Jeffrey T. Erickson, David E MillhornAbstract:A complete understanding of the neural mechanisms responsible for the chemoreceptor and baroreceptor reflexes requires precise knowledge of the locations and chemical phenotypes of higher-order neurons within these reflex pathways. In the present study, the protein product (Fos) of the c-fos protooncogene was used as a metabolic marker to trace central neural pathways following activation of carotid sinus nerve afferent fibers. In addition, immunohistochemical double-labeling techniques were used to define the chemical phenotypes of activated neurons. Both electrical stimulation of the carotid sinus nerve and physiological stimulation of the carotid bodies by hypoxia induced Fos-like immunoreactivity in catecholaminergic neurons containing tyrosine hydroxylase or phenylethanolamine-N-methyltransferase in the ventrolateral medulla oblongata and, to a lesser degree, in the dorsal vagal complex. Tyrosine hydroxylase/Fos colocalization was also observed in the locus coeruleus and the A5 noradrenergic cell group in pons. Many serotoninergic neurons in Nucleus Raphe Pallidus, Nucleus Raphe magnus, and along the ventral medullary surface contained Fos-like immunoreadtivity. In pons and midbrain, Fos-like immunoreactivity was observed in the lateral parabrachial and Kolliker–Fuse nuclei, the inferior colliculus, the cuneiform Nucleus, and in the vicinity of the Edinger–Westphal Nucleus, but no catecholaminergic or serotoninergic colocalization was observed in these regions. Although Fos-labeled cells were observed within and lateral to the dorsal Raphe Nucleus, few were catecholaminergic or serotoninergic. This study further defines a potential central neuroanatomical substrate for the chemoreceptor and/or baroreceptor reflexes. © 1994 Wiley-Liss, Inc.
-
Fos-like protein is induced in neurons of the medulla oblongata after stimulation of the carotid sinus nerve in awake and anesthetized rats.
Brain Research, 1991Co-Authors: Jeffrey T. Erickson, David E MillhornAbstract:Abstract The protooncogene c-fos is expressed rapidly, transiently and polysynaptically within neurons in response to synaptic activation and voltage-gated calcium entry into the cell. The nuclear protein product of this gene (Fos) is detectable immunohistochemically 20–90 min after cell activation and remains within the Nucleus for hours after expression. The present study was undertaken to identify cells within the rat medulla oblongata that express Fos-like protein in response to stimulation of afferent fibers of the carotoid sinus nerve (CSN). Direct electrical stimulation of the CSN in anesthetized animals or hypoxic stimulation in either anesthetized or awake animals resulted in a consistent and discrete distribution of Fos-like immunoreactivity (Fos-LI). Fos-LI was observed bilaterally within Nucleus tractus solitarius (NTS) and the ventrolateral medulla (VLM), within area postrema and Nucleus Raphe Pallidus, and bilaterally along the ventral medullary surface. Unstimulated animals were devoid of Fos-LI within the medulla oblongata. Furthermore, neither the surgical preparations alone nor the effects of anesthesia could account for the extent of Fos-LI observed. We believe these cells represent second- and higher-order neurons within the baroreceptor and chemoreceptor reflex pathways.
Richard B Lynn - One of the best experts on this subject based on the ideXlab platform.
-
Thyrotropin‐releasing hormone‐immunoreactive projections to the dorsal motor Nucleus and the Nucleus of the solitary tract of the rat
The Journal of Comparative Neurology, 1991Co-Authors: Richard B Lynn, Margaret S. Kreider, Richard R. MiselisAbstract:: Thyrotropin-releasing hormone-immunoreactive nerve terminals heavily innervate the dorsal motor Nucleus and Nucleus of the solitary tract, whereas cell bodies containing thyrotropin-releasing hormone residue most densely in the hypothalamus and Raphe nuclei. By using double-labeling techniques accomplished by retrograde transport of Fluoro-Gold following microinjection into the dorsal motor Nucleus/Nucleus of the solitary tract combined with immunohistochemistry for thyrotropin-releasing hormone, it was demonstrated that thyrotropin-releasing hormone-immunoreactive neurons projecting to the dorsal motor Nucleus/Nucleus of the solitary tract reside in the Nucleus Raphe Pallidus, Nucleus Raphe obscurus, and the parapyramidal region of the ventral medulla, but not in the paraventricular Nucleus of the hypothalamus. The parapyramidal region includes an area along the ventral surface of the caudal medulla, lateral to the pyramidal tract and inferior olivary Nucleus and ventromedial to the lateral reticular Nucleus. Varying the position of the Fluoro-Gold injection site revealed a rostral to caudal topographic organization of these Raphe and parapyramidal projections.
Yasuhiko Ibata - One of the best experts on this subject based on the ideXlab platform.
-
Catecholaminergic input to spinally projecting serotonin neurons in the rostral ventromedial medulla oblongata of the rat.
Brain research bulletin, 1994Co-Authors: Masaki Tanaka, Hitoshi Okamura, Yoshitaka Tamada, Ikuko Nagatsu, Yoshifumi Tanaka, Yasuhiko IbataAbstract:The midline of the rostral ventral medulla (RVM) is the portion in which many serotonin (5-HT) neurons of the Nucleus Raphe magnus and the rostral Nucleus Raphe Pallidus are located and where dense catecholaminergic (CA) fibers are distributed. In this study, we investigated the connection between spinally projecting 5-HT neurons and CA fibers in the rat RVM by light and electron microscopic immunocytochemistry. First, light microscopic immunocytochemistry using a triple labeling method revealed that the 5-HT-immunoreactive (IR) neuron containing retrograde tracer from the cervical cord was intimately surrounded by tyrosine hydroxylase (TH)-IR fibers. Second, silver-gold intensified TH-IR axon terminals were found to make synaptic contacts with 5-HT-IR neuronal perikarya and dendrites by double labeling immunoelectron microscopy. These morphological findings suggest that spinally projecting 5-HT neurons, presumed to be involved in pain modulation or sympathetic autonomic control, are directly regulated by CA neurons at the level of the RVM.
Jeffrey T. Erickson - One of the best experts on this subject based on the ideXlab platform.
-
Hypoxia and electrical stimulation of the carotid sinus nerve induce fos‐like immunoreactivity within catecholaminergic and serotoninergic neurons of the rat brainstem
The Journal of comparative neurology, 1994Co-Authors: Jeffrey T. Erickson, David E MillhornAbstract:A complete understanding of the neural mechanisms responsible for the chemoreceptor and baroreceptor reflexes requires precise knowledge of the locations and chemical phenotypes of higher-order neurons within these reflex pathways. In the present study, the protein product (Fos) of the c-fos protooncogene was used as a metabolic marker to trace central neural pathways following activation of carotid sinus nerve afferent fibers. In addition, immunohistochemical double-labeling techniques were used to define the chemical phenotypes of activated neurons. Both electrical stimulation of the carotid sinus nerve and physiological stimulation of the carotid bodies by hypoxia induced Fos-like immunoreactivity in catecholaminergic neurons containing tyrosine hydroxylase or phenylethanolamine-N-methyltransferase in the ventrolateral medulla oblongata and, to a lesser degree, in the dorsal vagal complex. Tyrosine hydroxylase/Fos colocalization was also observed in the locus coeruleus and the A5 noradrenergic cell group in pons. Many serotoninergic neurons in Nucleus Raphe Pallidus, Nucleus Raphe magnus, and along the ventral medullary surface contained Fos-like immunoreadtivity. In pons and midbrain, Fos-like immunoreactivity was observed in the lateral parabrachial and Kolliker–Fuse nuclei, the inferior colliculus, the cuneiform Nucleus, and in the vicinity of the Edinger–Westphal Nucleus, but no catecholaminergic or serotoninergic colocalization was observed in these regions. Although Fos-labeled cells were observed within and lateral to the dorsal Raphe Nucleus, few were catecholaminergic or serotoninergic. This study further defines a potential central neuroanatomical substrate for the chemoreceptor and/or baroreceptor reflexes. © 1994 Wiley-Liss, Inc.
-
Fos-like protein is induced in neurons of the medulla oblongata after stimulation of the carotid sinus nerve in awake and anesthetized rats.
Brain Research, 1991Co-Authors: Jeffrey T. Erickson, David E MillhornAbstract:Abstract The protooncogene c-fos is expressed rapidly, transiently and polysynaptically within neurons in response to synaptic activation and voltage-gated calcium entry into the cell. The nuclear protein product of this gene (Fos) is detectable immunohistochemically 20–90 min after cell activation and remains within the Nucleus for hours after expression. The present study was undertaken to identify cells within the rat medulla oblongata that express Fos-like protein in response to stimulation of afferent fibers of the carotoid sinus nerve (CSN). Direct electrical stimulation of the CSN in anesthetized animals or hypoxic stimulation in either anesthetized or awake animals resulted in a consistent and discrete distribution of Fos-like immunoreactivity (Fos-LI). Fos-LI was observed bilaterally within Nucleus tractus solitarius (NTS) and the ventrolateral medulla (VLM), within area postrema and Nucleus Raphe Pallidus, and bilaterally along the ventral medullary surface. Unstimulated animals were devoid of Fos-LI within the medulla oblongata. Furthermore, neither the surgical preparations alone nor the effects of anesthesia could account for the extent of Fos-LI observed. We believe these cells represent second- and higher-order neurons within the baroreceptor and chemoreceptor reflex pathways.
