The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Liu Hongche - One of the best experts on this subject based on the ideXlab platform.
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changes of pulp vessels and cgrp immunoreactive fibres induced by Occlusal Trauma
Chinese Journal of Prosthodontics, 2012Co-Authors: Liu HongcheAbstract:Objective: To investigate the effect of Occlusal Trauma on the pulp CGRP-immunoreactice fibres and pulp blood vessels in rat,and observe the relation between pulp nerves and blood vessels.Methods: The morphology of CGRP-immunoreactive fibres and blood vessels in right first mandibular molar pulp tissue of Occlusal Trauma,inferior alveolar nerve(IAN) resection and control group in rat were compared using HE staining and Immunohistochemical staining.Results: In the group of Occlusal Trauma from 3 to 30 days,vasodilation in pulp progressively enhanced and the density of CGRP-positive fibres gradually increased in the cell free zone.CGRP-immunoreactive expression enhanced in the capillary area,and CGRP-immunoreactive expression increased in the region of inflammatory infiltration and vasodilation.In Occlusal Trauma 60-90 days group,because of the degeneration and necrosis of pulpal tissue,CGRP-immunoreactive expression gradually reduced in the cell free zone,but still without recovering to the level of control.Degenerative and necrotic range of pulp expanded,and vasodilative area were observed to shrank relatively.In IAN resection group,at 30 days of Occlusal Trauma,CGRP-immunoreactive fibres showed hypochromic granule and short filaments in the cell free zone.Vasodilation in pulp was not present.CGRP-immunoreactive fibre in one case bound in pulp chamber and appeared dense tree-like structure;the pulp vasodilation was obvious in relevant area.Conclusions: Occlusal Trauma could change the morphology of the nerve and cause the neurogenic inflammation.The change of pulp circulation following Occlusal Trauma was confirmed to consistent with the distribution and density of nerve fibers,which might be a mechanism of pulposis induced of Occlusal Trauma.
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response of astrocytes to Occlusal Trauma in spinal trigeminal subnucleus caudalis
Chinese Journal of Prosthodontics, 2010Co-Authors: Liu HongcheAbstract:Objective: To investigate the role of astrocyte in spinal trigeminal subnucleus caudalis(Sp5C)during Occlusal Trauma.Methods: The Occlusal surface of the left first maxillary molars were raised 0.5mm with the square steel to establish Traumatic occlusion animal models.On 1,3,7,14,30d after teeth operation,Sp5C were removed.Immunofluorescence histochemical was used to qualitively examine the distribution of GFAP in the Sp5C during the Occlusal Trauma.Results: Astrocytes in the Sp5C of control group occasionally had GFAP-IR.The number of GFAP-containing astrocytes increased in Sp5C at 3d after Occlusal Trauma,the maximum density of GFAP immunoreactivity in experimental side was found at 7d and decreased at 14d.Conclusion: The increase in numbers of astrocytes implied the possible involvement of astrocytes in Traumatic Occlusal pain.
Liu Xueme - One of the best experts on this subject based on the ideXlab platform.
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the changes of c fos to Occlusal Trauma in rat spinal trigeminal subnucleus candalis
Chinese Journal of Prosthodontics, 2010Co-Authors: Liu XuemeAbstract:Objective: To investigate the changes of c-fos in the Sp5C during Occlusal Trauma and study the effect of Occlusal Trauma on neurons.Methods: The Occlusal surface of the left first maxillary molar was raised 0.5mm with the square wire.On 1,3,7d,2w,4w and 5w after teeth operation,immunofluorescence histochemical was used to examine the number of c-fos contanining neurons in the Sp5C.Results: The number of c-fos-containing neurons increased significantly in experimental side of Sp5C at 7d after Occlusal Trauma;the maximum number of c-fos contanining neurons was found at 2w after Occlusal Trauma and returned to the control level at 4w.Conclusion: The neurons in rats' Sp5C were highly active during Occlusal Trauma.
Duan Liju - One of the best experts on this subject based on the ideXlab platform.
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expression of ppta mrna in trigeminal ganglion in rat during Occlusal Trauma
Chinese Journal of Prosthodontics, 2002Co-Authors: Duan LijuAbstract:Objective: To survey expression of PPTA-mRNA in trigeminal ganglion in rat during persistent OcclusalTrauma. Methods: The model of Occlusal Trauma was established in rat. Adopting molecular hybridization technique, theexpression of PPTA-mRNA in trigeminal ganglion was observed at 7, 15 and 30 days following Occlusal Trauma. Results:During persistent Occlusal Trauma, expression of PPTA-mRNA was more significant than that of control group. Expressionincrease of PPTA-mRNA during Occlusal Trauma was higher than 15 and 30 days groups. Expression had no significantdifference between 15 and 30 days groups. Conclusions: During Occlusal Trauma, expression of PPTA-mRNA in trigeminalganglion increased, which suggests that synthesis of precursors of substance P increase in primary sensory neurons.
Kaiyuan Fu - One of the best experts on this subject based on the ideXlab platform.
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ultrastructural changes of tmj articular cartilage and synovial membrane following Occlusal Trauma in rabbit
Chinese journal of traumatology, 1999Co-Authors: Kaiyuan Fu, Zhenkang Zhang, Jin WangAbstract:: OBJECTIVE: To study the effects of Occlusal Trauma on the ultra-structure of synovial membrane and articular cartilage in rabbit's temporomandibular joints (TMJ). METHODS: TMJs from six rabbits with Occlusal Trauma and three control rabbits were studied by transmission electron microscopy. RESULTS: Degenerative changes in synovial membrane and articular cartilage of TMJ were induced following Occlusal Trauma. The structure of the articular surface was damaged, and chondrocytes in cartilage showed signs of degeneration. The synovial lining cells contained dense accumulations of vimentin intermediate filaments (IFs), which were especially prevalent in the cellular processes as well as paranuclearly. Microvilli on the synovial cell membrane were commonly seen. The "vermiform bodies" in the deeper interstitium of the synovial tissue were also found. Our findings of the punctate adherens between synovial lining cells were described in detail. CONCLUSIONS: The Occlusal Trauma is really a factor inducing degenerative changes of the TMJ.
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Studies on contributing factors in temporomandibular disorders.
The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association, 1999Co-Authors: Zu-yan Zhang, Ma Xc, Gao S, Gu Zy, Kaiyuan FuAbstract:OBJECTIVE: To better understand the pathogenesis of TMD by studying microTrauma of the temporomandibular joint (TMJ), immune responses within TMJ, and psychosocial factors of the past ten years. METHODS: Condyle and disc movements from 38 patients with temporomandibular disorders (TMD) were observed with the use of videotape recording and soundtape recording techniques after TMJ arthrography. Pathological changes following Occlusal Trauma were examined using an animal model. Immune complexes in condyle cartilage; antibody to collagen II; and cytokines such as interleukin 1, tumor necrosis factor, and interleukin 6 were detected in synovial fluid of TMD. Psychosocial characteristics were analyzed with the Minnesota Multiphasic Personality Inventory (MMPI) of 80 TMD patients and the Life Events Experience Survey (LEES) of 42 TMD patients. RESULTS: Persistent and recurrent microTrauma did exist within joints of TMD patients, caused by Occlusal Trauma and Occlusal interference. Occlusal Trauma in animals could induce condyle and disc degenerative changes that are similar to the findings in TMJ osteoarthrosis patients. Sequestered antigens within cartilage could be exposed to the immune system after joint degeneration. Humoral and cellular immune responses did exist within TMJ and played an important role in the pathogenesis of TMD. Forty percent of TMD patients suffered from psychosomatic disorders, significantly more than in the healthy control. CONCLUSION: MicroTrauma of TMJ, immune responses within TMJ, psychosocial factors, and anatomical structures of the TMJ itself are the four main contributing factors of TMD. Possible mechanisms of the interactions of the four factors are presented, and principles of preventing and treating TMD are also suggested.
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ultrastructure of the synovial membrane and the articular cartilage in the temporomandibular joint of the rabbits with Occlusal Trauma
Chinese journal of stomatology, 1998Co-Authors: Kaiyuan Fu, X C, Z ZhangAbstract:OBJECTIVE: To study the effect of Occlusal Trauma on the ultra structure of rabbits' temporomandibular joints (TMJ) synovial membrane and articular cartilage. METHODS: TMJs from six rabbits with Occlusal Trauma and three control rabbits were studied by transmission electron microscopy. RESULTS: Degenerative changes of TMJ tissues were found. The articular surface of the condyle was damaged, and the chondrocytes showed signs of degeneration. The synovial lining cell consisted of a number of vimentin intermediate filaments (IFs) which were especially prevalent in the processes as well as paranuclearly. Microvilli on the cell membrane were commonly seen. The vermiform body in the deeper interstitium was also found. Our finding of punctate adherens between synoviocytes was firstly reported in the study. CONCLUSION: The Occlusal Trauma is really a factor inducing degenerative changes of the TMJ.
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effects of Occlusal Trauma on temporomandibular joints in rabbits a scanning electron microscopic study
Chinese journal of stomatology, 1995Co-Authors: Kaiyuan Fu, X C, Z ZhangAbstract:: Six rabbits with unilateral Occlusal splints on the mandibular left molars for one month, three months or five months were examined to study the ultrastructure of the surface of the temporomandibular joint. Four rabbits were used as control. The results showed that the surface layer of the disk and articular cartilage were damaged. Severe fibrillation with sheets of fibers, collagen fibrils and collagen bundles inside fissures and craters were observed. These changes were seen in all rabbits with Occlusal Trauma and also in control rabbits for five months. The findings suggested that Occlusal Trauma could result in degenerative changes of the temporomandibular joint.
Antonio Wilson Sallum - One of the best experts on this subject based on the ideXlab platform.
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cigarette smoke inhalation increases the alveolar bone loss caused by primary Occlusal Trauma in a rat model
Journal of Periodontal Research, 2014Co-Authors: Mirella Gomes L Campos, Marcio Zaffalon Casati, Enilson Antonio Sallum, Francisco Humberto Nociti, Monica Grazieli Correa, Antonio Wilson SallumAbstract:Background and Objective Occlusal Trauma (OT) and smoking are both factors that alter alveolar bone metabolism and therefore could synergistically act on alveolar bone loss. The aim of this experimental study was to evaluate the influence of short-term cigarette smoke inhalation (CSI) on inter-radicular alveolar bone loss promoted by primary OT in a rat model. Material and Methods Forty-eight animals were randomly assigned to one of three groups based on treatment type: OT + CSI (n = 16), animals were exposed to CSI three times per day, for 8 min per exposure, and they concomitantly received unilateral vertical augmentation creating an Occlusal interference inducing experimental OT; OT (n = 16), animals received only unilateral vertical augmentation; negative control (NC; n = 16), animals maintained for equal periods to achieve periodontal baseline values of periodontal ligament dimension. Each group was divided into two subgroups (n = 8) based on treatment length: 7 or 14 d. Results After 7 d, the OT + CSI group exhibited significantly higher bone loss compared to the NC group (p = 0.0022). After 14 d, the OT (p < 0.0001) and OT + CSI (p < 0.0001) groups presented significantly higher bone loss compared to the NC group, and OT + CSI resulted in significantly higher bone loss than OT alone (p = 0.0241). The number of tartrate-resistant acid phosphatase-positive cells on the linear surface of the bone crest after 7 d was significantly higher in the OT + CSI group as compared to the NC and OT groups (p < 0.0001 and p = 0.0045, respectively) and remained significantly higher in the OT + CSI group after 14 d, compared to the OT group (p < 0.0001). Conclusion Short-term CSI increases early bone loss in association with OT after 7 d, and this worsens in severity after 14 d of exposure.
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Cigarette smoke inhalation increases the alveolar bone loss caused by primary Occlusal Trauma in a rat model
Journal of Periodontal Research, 2013Co-Authors: Mirella L. Gomes Campos, Marcio Zaffalon Casati, Enilson Antonio Sallum, Monica Grazieli Correa, Francisco Humberto Nociti Junior, Antonio Wilson SallumAbstract:Background and Objective Occlusal Trauma (OT) and smoking are both factors that alter alveolar bone metabolism and therefore could synergistically act on alveolar bone loss. The aim of this experimental study was to evaluate the influence of short-term cigarette smoke inhalation (CSI) on inter-radicular alveolar bone loss promoted by primary OT in a rat model. Material and Methods Forty-eight animals were randomly assigned to one of three groups based on treatment type: OT + CSI (n = 16), animals were exposed to CSI three times per day, for 8 min per exposure, and they concomitantly received unilateral vertical augmentation creating an Occlusal interference inducing experimental OT; OT (n = 16), animals received only unilateral vertical augmentation; negative control (NC; n = 16), animals maintained for equal periods to achieve periodontal baseline values of periodontal ligament dimension. Each group was divided into two subgroups (n = 8) based on treatment length: 7 or 14 d. Results After 7 d, the OT + CSI group exhibited significantly higher bone loss compared to the NC group (p = 0.0022). After 14 d, the OT (p
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nicotine effects on alveolar bone changes induced by Occlusal Trauma a histometric study in rats
Journal of Periodontology, 2004Co-Authors: Getulio Nogueirafilho, Edgard Froes B Neto, Marcio Zaffalon Casati, Silvia R A Reis, Roberto S Tunes, Urbino Da R Tunes, Enilson Antonio Sallum, Francisco Humberto Nociti, Antonio Wilson SallumAbstract:BACKGROUND: The aim of the present study was to verify nicotine effects on alveolar bone changes induced by Occlusal Trauma during a periodontitis experimental model in rats. METHODS: Thirty adult male rats were used. The animals were randomly assigned to one of three groups receiving daily intraperitoneal injections: A, nicotine solution (0.44 mg/ml) and Occlusal overload; B, saline solution and Occlusal overload; or C, saline solution. Rats from groups A and B underwent bilateral amputation of the second and third molar cusps to simulate an Occlusal overload. The first molars were then randomly assigned to receive a cotton ligature in the sulcular area, while the contralateral tooth was left unligated. The animals were sacrificed 30 days later. The resected mandibles were processed, and histomorphometric measurements were performed in the alveolar bone adjacent to the furcation area of the first molars. RESULTS: Nicotine enhanced the bone loss induced by Occlusal Trauma (P<0.001) on the ligated teeth of group A (12.27 +/- 4.4 mm2), when compared to groups B (8.43 +/- 3.51 mm2) and C (4.43 +/- 2.17 mm2). Alveolar bone loss (P<0.01) was also observed in the contralateral teeth of groups A (nicotine + Trauma) and B (saline + Trauma), when compared to group C (saline only). CONCLUSION: Within the limits of the study, it is concluded that nicotine may influence the alveolar bone changes induced by Occlusal Trauma by enhancing bone loss.
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Nicotine effects on alveolar bone changes induced by Occlusal Trauma: a histometric study in rats.
Journal of Periodontology, 2004Co-Authors: Getulio Nogueira-filho, Marcio Zaffalon Casati, Silvia R A Reis, Roberto S Tunes, Urbino Da R Tunes, Enilson Antonio Sallum, Francisco Humberto Nociti, Edgard B. Fróes Neto, Antonio Wilson SallumAbstract:BACKGROUND: The aim of the present study was to verify nicotine effects on alveolar bone changes induced by Occlusal Trauma during a periodontitis experimental model in rats. METHODS: Thirty adult male rats were used. The animals were randomly assigned to one of three groups receiving daily intraperitoneal injections: A, nicotine solution (0.44 mg/ml) and Occlusal overload; B, saline solution and Occlusal overload; or C, saline solution. Rats from groups A and B underwent bilateral amputation of the second and third molar cusps to simulate an Occlusal overload. The first molars were then randomly assigned to receive a cotton ligature in the sulcular area, while the contralateral tooth was left unligated. The animals were sacrificed 30 days later. The resected mandibles were processed, and histomorphometric measurements were performed in the alveolar bone adjacent to the furcation area of the first molars. RESULTS: Nicotine enhanced the bone loss induced by Occlusal Trauma (P
