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Ricardo Santiago Gomez - One of the best experts on this subject based on the ideXlab platform.
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abstract 153 reduction of micrornas mir 15a and mir 16 1 in Odontogenic Keratocyst
Cancer Research, 2011Co-Authors: Ricardo Santiago Gomez, Carolina Cavalieri Gomes, Marina Goncalves Diniz, Wagner Henriques De Castro, Andre Luiz Sena Guimaraes, Alfredo Mauricio Batista De PaulaAbstract:Odontogenic Keratocyst (OKC) is a benign destructive recurrent Odontogenic cystic neoplasm. MicroRNAs (miRNAs) miR-15a and miR-16-1 are small non-protein coding RNAs that function as negative regulators of the anti-apoptotic gene BCL-2 at the post-transcriptional level. Increased immunoexpression of BCL-2 protein is reported in OKC. The purpose of the present study was to investigate whether miR-15a and miR-16-1 transcription is altered in OKC and if it is associated with BCL-2 expression in such lesions. Compared to dental follicles, we found higher BCL-2 mRNA expression and immunoreactivity in OKC. Our findings also showed that miR-15a and/or miR-16-1 are downregulated in the majority of OKC samples (24/28). Furthermore, in all five OKC paired samples (primary and marsupialized lesions) investigated there was an increase in the expression of miR-15a after marsupialization. Our results suggest that OKC neoplastic cells present an anti-apoptotic profile to which lower miR-16-15a expression might contribute. Additionally, our findings strongly suggest that marsupialization reduces BCL-2 expression in the OKC and this downregulation may result from microRNA post-transcriptional activity, even though this cause-effect relationship remains to be proved. This study was supported by FAPEMIG and CAPES, Brazil. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 153. doi:10.1158/1538-7445.AM2011-153
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review of the molecular pathogenesis of the Odontogenic Keratocyst
Oral Oncology, 2009Co-Authors: Carolina Cavalieri Gomes, Marina Goncalves Diniz, Ricardo Santiago GomezAbstract:The Odontogenic Keratocyst (Keratocystic Odontogenic tumour) (OKC) is one of the most prevalent Odontogenic tumours. Since its initial description, a number of studies have focused on different aspects of this lesion, attempting to explain its distinctive biological behaviour. In this review the authors address the main genetic and epigenetic alterations reported on this tumour. Although most of the knowledge on this field is not being used in the clinical practice, some perspectives of translational studies are discussed.
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Odontogenic Keratocyst in the maxillary sinus report of two cases
Oral Oncology Extra, 2006Co-Authors: Guilherme Costa Silva, Ricardo Santiago Gomez, Edgard Carvalho Silva, Tainah Couto VieiraAbstract:Summary The Odontogenic Keratocyst (OKC) is well known for its tendency to recur, potential aggressive behaviour and defined histopathological feature. OKC occurrence in the maxilla is unusual and its appearance in the maxillary sinus very uncommon. This article reports two distinct cases of OKCs associated with unerupted molars in the maxillary sinus of two boys. The lesions were surgically treated and no recurrence has been observed on follow-up. OKC clinical features and treatment are discussed.
Federica Rizza - One of the best experts on this subject based on the ideXlab platform.
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Case Report Odontogenic Keratocyst Mimicking Paradental Cyst
2016Co-Authors: Andrea Enrico Borgonovo, Luigi Bernardini, Paola Francinetti, Federica RizzaAbstract:Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Objective. The aim of this paper is to present an uncommon clinical and radiographic aspect of Odontogenic Keratocyst (OKC) mimicking paradental cyst.Methods. A 32-year-old female patient showed a well-delimited radiolucent lesion connected with the root of the left thirdmolar with close anatomical relationship with themandibular canal.The clinical, radiographic, and anamnestic features lead us to diagnose a paradental cyst that was treated by enucleation after extraction of the partially impacted tooth.Results. Histological analysis showed typical histological features of PKC such as the presence of a lining of stratified squamous epithelium with a well-defined basal layer of palisading columnar of cuboidal cells. Conclusion. Initial X-ray analysis and the position of the lesion related to the third mandibular tooth caused us to mistakenly diagnose a paradental cyst. We were only able to identify the cyst as an PKC rather than a paradental cyst after histological analysis. 1
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Odontogenic Keratocyst mimicking paradental cyst
Case Reports in Dentistry, 2014Co-Authors: Andrea Enrico Borgonovo, Luigi Bernardini, Paola Francinetti, Federica RizzaAbstract:Objective. The aim of this paper is to present an uncommon clinical and radiographic aspect of Odontogenic Keratocyst (OKC) mimicking paradental cyst. Methods. A 32-year-old female patient showed a well-delimited radiolucent lesion connected with the root of the left third molar with close anatomical relationship with the mandibular canal. The clinical, radiographic, and anamnestic features lead us to diagnose a paradental cyst that was treated by enucleation after extraction of the partially impacted tooth. Results. Histological analysis showed typical histological features of PKC such as the presence of a lining of stratified squamous epithelium with a well-defined basal layer of palisading columnar of cuboidal cells. Conclusion. Initial X-ray analysis and the position of the lesion related to the third mandibular tooth caused us to mistakenly diagnose a paradental cyst. We were only able to identify the cyst as an PKC rather than a paradental cyst after histological analysis.
Lorenzo Preda - One of the best experts on this subject based on the ideXlab platform.
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Odontogenic Keratocyst imaging features of a benign lesion with an aggressive behaviour
Insights Into Imaging, 2018Co-Authors: Andrea Borghesi, Cosimo Nardi, Caterina Giannitto, Andrea Tironi, Roberto Maroldi, Francesco Di Bartolomeo, Lorenzo PredaAbstract:The latest (4th) edition of the World Health Organization (WHO) Classification of Head and Neck Tumours, published in January 2017, has reclassified Keratocystic Odontogenic tumour as Odontogenic Keratocyst. Therefore, Odontogenic Keratocysts (OKCs) are now considered benign cysts of Odontogenic origin that account for about 10% of all Odontogenic cysts. OKCs arise from the dental lamina and are characterised by a cystic space containing desquamated keratin with a uniform lining of parakeratinised squamous epithelium. The reported age distribution of OKCs is considerably wide, with a peak of incidence in the third decade of life and a slight male predominance. OKCs originate in tooth-bearing regions and the mandible is more often affected than the maxilla. In the mandible, the most common location is the posterior sextant, the angle or the ramus. Conversely, the anterior sextant and the third molar region are the most common sites of origin in the maxilla. OKCs are characterised by an aggressive behaviour with a relatively high recurrence rate, particularly when OKCs are associated with syndromes. Multiple OKCs are typically associated with the nevoid basal cell carcinoma syndrome (NBCCS), an autosomal dominant multisystemic disease. Radiological imaging, mainly computed tomography (CT) and, in selected cases, magnetic resonance imaging (MRI), plays an important role in the diagnosis and management of OKCs. Therefore, the main purpose of this pictorial review is to present the imaging appearance of OKCs underlining the specific findings of different imaging modalities and to provide key radiologic features helping the differential diagnoses from other cystic and neoplastic lesions of Odontogenic origin. • Panoramic radiography is helpful in the preliminary assessment of OKCs. • CT is considered the tool of choice in the evaluation of OKCs. • MRI with DWI or DKI can help differentiate OKCs from other Odontogenic lesions. • Ameloblastoma, dentigerous and radicular cysts should be considered in the differential diagnosis. • The presence of multiple OKCs is one of the major criteria for the diagnosis of NBCCS.
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Odontogenic Keratocyst: imaging features of a benign lesion with an aggressive behaviour
SpringerOpen, 2018Co-Authors: Andrea Borghesi, Cosimo Nardi, Caterina Giannitto, Andrea Tironi, Roberto Maroldi, Francesco Di Bartolomeo, Lorenzo PredaAbstract:Abstract The latest (4th) edition of the World Health Organization (WHO) Classification of Head and Neck Tumours, published in January 2017, has reclassified Keratocystic Odontogenic tumour as Odontogenic Keratocyst. Therefore, Odontogenic Keratocysts (OKCs) are now considered benign cysts of Odontogenic origin that account for about 10% of all Odontogenic cysts. OKCs arise from the dental lamina and are characterised by a cystic space containing desquamated keratin with a uniform lining of parakeratinised squamous epithelium. The reported age distribution of OKCs is considerably wide, with a peak of incidence in the third decade of life and a slight male predominance. OKCs originate in tooth-bearing regions and the mandible is more often affected than the maxilla. In the mandible, the most common location is the posterior sextant, the angle or the ramus. Conversely, the anterior sextant and the third molar region are the most common sites of origin in the maxilla. OKCs are characterised by an aggressive behaviour with a relatively high recurrence rate, particularly when OKCs are associated with syndromes. Multiple OKCs are typically associated with the nevoid basal cell carcinoma syndrome (NBCCS), an autosomal dominant multisystemic disease. Radiological imaging, mainly computed tomography (CT) and, in selected cases, magnetic resonance imaging (MRI), plays an important role in the diagnosis and management of OKCs. Therefore, the main purpose of this pictorial review is to present the imaging appearance of OKCs underlining the specific findings of different imaging modalities and to provide key radiologic features helping the differential diagnoses from other cystic and neoplastic lesions of Odontogenic origin. Key Points • Panoramic radiography is helpful in the preliminary assessment of OKCs. • CT is considered the tool of choice in the evaluation of OKCs. • MRI with DWI or DKI can help differentiate OKCs from other Odontogenic lesions. • Ameloblastoma, dentigerous and radicular cysts should be considered in the differential diagnosis. • The presence of multiple OKCs is one of the major criteria for the diagnosis of NBCCS
Indraneel Bhattacharyya - One of the best experts on this subject based on the ideXlab platform.
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report of two cases of combined Odontogenic tumors ameloblastoma with Odontogenic Keratocyst and ameloblastic fibroma with calcifying Odontogenic cyst
Head and Neck Pathology, 2015Co-Authors: Ashley N Neuman, L Montague, Donald M Cohen, Nadim M Islam, Indraneel BhattacharyyaAbstract:Combined Odontogenic neoplasms have rarely been documented. Such tumors have also been described by other researchers as “hybrid” lesions. The histologic features are often identical to other individually well-established Odontogenic neoplasms such as ameloblastoma, adenomatoid Odontogenic tumor, ameloblastic fibroma (AF), and ameloblastic fibro-odontoma. Their clinical presentation is variable, ranging from cysts to neoplasms showing varying degrees of aggressive behavior. Most combined tumors contain features of one of the Odontogenic tumors in combination with either a calcifying Odontogenic cyst (COC) or a calcifying epithelial Odontogenic tumor. We present two new cases of combined Odontogenic tumors: an ameloblastoma with an Odontogenic Keratocyst and an AF with COC. Predicting clinical outcome is challenging when a combination tumor is encountered due to the paucity of such lesions. One must understand salient features of these entities and differentiate them from the more common conventional neoplasms to expand classification and provide prognostic criteria.
Gerhard R - One of the best experts on this subject based on the ideXlab platform.
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Fine needle aspiration cytology as an additional tool in the diagnosis of Odontogenic Keratocyst
'Wiley', 2015Co-Authors: Pa Vargas, G M Mata, A V Jones, Perez Ded, Op ,de Almeida, Gerhard RAbstract:Fine needle aspiration cytology as an additional tool in the diagnosis of Odontogenic Keratocyst The aim of this study was to assess the use of fine needle aspiration cytology (FNAC) in diagnosis of Odontogenic Keratocyst (OKC), as well as to describe the cytological and immunohistochemical features. Eight consecutive patients submitted to FNAC and diagnosed with OKC were included in this study. FNAC was performed using 24-gauge needles attached to a 10-ml syringe, supported by a mechanical-syringe holder to facilitate aspiration. All cases provided a liquid or viscous content for smears that were either air-dried for Diff-Quick((R)) staining or immediately fixed in 95% alcohol and stained by the Papanicolaou technique. Incisional biopsies were carried out to confirm the diagnosis. Immunohistochemical reactions against anti-pan-cytokeratin (CK), CK14 and CK19 were performed in 3 mu m sections obtained from cell blocks and biopsy specimens. Cytologically many isolated or groups of keratinocytes with normal or ill defined nuclei were seen, besides numerous anucleated squamous cells and keratinous debris. Immunohistochemically, the keratin lamellae were positive for pan-cytokeratin and CK19, but negative for CK14. In biopsy specimens, CK14 expression was restricted to basal cells, while only the superficial cells were positive for CK19. In summary, FNAC is useful, reliable and safe tool for the preoperative diagnosis of OKC.18636136
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Fine Needle Aspiration Cytology As An Additional Tool In The Diagnosis Of Odontogenic Keratocyst.
'Wiley', 2015Co-Authors: Vargas P A, Da Cruz Perez D E, Mata G M, De Almeida O P, Jones A, Gerhard RAbstract:The aim of this study was to assess the use of fine needle aspiration cytology (FNAC) in diagnosis of Odontogenic Keratocyst (OKC), as well as to describe the cytological and immunohistochemical features. Eight consecutive patients submitted to FNAC and diagnosed with OKC were included in this study. FNAC was performed using 24-gauge needles attached to a 10-ml syringe, supported by a mechanical-syringe holder to facilitate aspiration. All cases provided a liquid or viscous content for smears that were either air-dried for Diff-Quick staining or immediately fixed in 95% alcohol and stained by the Papanicolaou technique. Incisional biopsies were carried out to confirm the diagnosis. Immunohistochemical reactions against anti-pan-cytokeratin (CK), CK14 and CK19 were performed in 3 microm sections obtained from cell blocks and biopsy specimens. Cytologically many isolated or groups of keratinocytes with normal or ill defined nuclei were seen, besides numerous anucleated squamous cells and keratinous debris. Immunohistochemically, the keratin lamellae were positive for pan-cytokeratin and CK19, but negative for CK14. In biopsy specimens, CK14 expression was restricted to basal cells, while only the superficial cells were positive for CK19. In summary, FNAC is useful, reliable and safe tool for the preoperative diagnosis of OKC.18361-
