The Experts below are selected from a list of 61338 Experts worldwide ranked by ideXlab platform
Kazuya Watanabe - One of the best experts on this subject based on the ideXlab platform.
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sulfurospirillum cavolei sp nov a facultatively anaerobic sulfur reducing bacterium isolated from an underground crude Oil Storage cavity
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Yumiko Kodama, Le Thu Ha, Kazuya WatanabeAbstract:A novel facultatively anaerobic sulfur-reducing bacterium, designated strain Phe91T, was isolated from petroleum-contaminated groundwater in an underground crude Oil Storage cavity at Kuji in Iwate, Japan. Cells of strain Phe91T were slightly curved rods with single polar flagella. Optimum growth was observed at pH 7.0 and 30 °C. The novel strain utilized elemental sulfur, thiosulfate, sulfite, dithionite, arsenate, nitrate and DMSO as electron acceptors with lactate as an energy and carbon source, but nitrite was not utilized. Microaerophilic growth was also observed. Fumarate, pyruvate, lactate, malate, succinate, hydrogen (with acetate as a carbon source) and formate (with acetate) could serve as electron donors. Fumarate, pyruvate and malate were fermented. The DNA G+C content was 42.7 mol%. On the basis of 16S rRNA gene sequence phylogeny, strain Phe91T was affiliated with the genus Sulfurospirillum in the class Epsilonproteobacteria and was most closely related to Sulfurospirillum deleyianum (sequence similarity 97 %). However, the DNA–DNA hybridization value between strain Phe91T and S. deleyianum was only 14 %. Based on the physiological and phylogenetic data, Phe91T should be classified as a representative of a novel species in the genus Sulfurospirillum; the name Sulfurospirillum cavolei sp. nov. is proposed, with Phe91T (=JCM 13918T=DSM 18149T) as the type strain.
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sulfuricurvum kujiense gen nov sp nov a facultatively anaerobic chemolithoautotrophic sulfur oxidizing bacterium isolated from an underground crude Oil Storage cavity
International Journal of Systematic and Evolutionary Microbiology, 2004Co-Authors: Yumiko Kodama, Kazuya WatanabeAbstract:A facultatively anaerobic, chemolithoautotrophic, sulfur-oxidizing bacterium, strain YK-1T, was isolated from an underground crude-Oil Storage cavity at Kuji in Iwate, Japan. The cells were motile, curved rods and had a single polar flagellum. Optimum growth occurred in a low-strength salt medium at pH 7·0 and 25 °C. It utilized sulfide, elemental sulfur, thiosulfate and hydrogen as the electron donors and nitrate as the electron acceptor under anaerobic conditions, but it did not use nitrite. Oxygen also served as the electron acceptor under the microaerobic condition (O2 in the head space 1 %). It did not grow on sugars, organic acids or hydrocarbons as carbon and energy sources. The DNA G+C content of strain YK-1T was 45 mol%. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that its closest relative was Thiomicrospira denitrificans in the ‘Epsilonproteobacteria’, albeit with low homology (90 %). On the basis of physiological and phylogenetic data, strain YK-1T should be classified into a novel genus and species, for which the name Sulfuricurvum kujiense gen. nov., sp. nov. is proposed. The type strain is YK-1T (=JCM 11577T=MBIC 06352T=ATCC BAA-921T).
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sulfuricurvum kujiense gen nov sp nov a facultatively anaerobic chemolithoautotrophic sulfur oxidizing bacterium isolated from an underground crude Oil Storage cavity
International Journal of Systematic and Evolutionary Microbiology, 2004Co-Authors: Yumiko Kodama, Kazuya WatanabeAbstract:A facultatively anaerobic, chemolithoautotrophic, sulfur-oxidizing bacterium, strain YK-1(T), was isolated from an underground crude-Oil Storage cavity at Kuji in Iwate, Japan. The cells were motile, curved rods and had a single polar flagellum. Optimum growth occurred in a low-strength salt medium at pH 7.0 and 25 degrees C. It utilized sulfide, elemental sulfur, thiosulfate and hydrogen as the electron donors and nitrate as the electron acceptor under anaerobic conditions, but it did not use nitrite. Oxygen also served as the electron acceptor under the microaerobic condition (O(2) in the head space 1 %). It did not grow on sugars, organic acids or hydrocarbons as carbon and energy sources. The DNA G+C content of strain YK-1(T) was 45 mol%. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that its closest relative was Thiomicrospira denitrificans in the 'Epsilonproteobacteria', albeit with low homology (90 %). On the basis of physiological and phylogenetic data, strain YK-1(T) should be classified into a novel genus and species, for which the name Sulfuricurvum kujiense gen. nov., sp. nov. is proposed. The type strain is YK-1(T) (=JCM 11577(T)=MBIC 06352(T)=ATCC BAA-921(T)).
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diversity abundance and activity of archaeal populations in Oil contaminated groundwater accumulated at the bottom of an underground crude Oil Storage cavity
Applied and Environmental Microbiology, 2002Co-Authors: Kazuya Watanabe, Yumiko Kodama, Natsuko Hamamura, Nobuo KakuAbstract:Fluorescence in situ hybridization has shown that cells labeled with an Archaea-specific probe (ARCH915) accounted for approximately 10% of the total cell count in Oil-contaminated groundwater accumulated at the bottom of an underground crude Oil Storage cavity. Although chemical analyses have revealed vigorous consumption of nitrate in cavity groundwater, the present study found that the methane production rate was higher than the nitrate consumption rate. To characterize the likely archaeal populations responsible for methane production in this system, fragments of 16S ribosomal DNA (rDNA) were amplified by PCR using eight different combinations of universal and Archaea-specific primers. Sequence analysis of 324 clones produced 23 different archaeal sequence types, all of which were affiliated with the kingdom Euryarchaeota. Among them, five sequence types (KuA1, KuA6, KuA12, KuA16, and KuA22) were obtained in abundance. KuA1 and KuA6 were closely related to the known methanogens Methanosaeta concilii (99% identical) and Methanomethylovorans hollandica (98%), respectively. Although no closely related organism was found for KuA12, it could be affiliated with the family Methanomicrobiaceae. KuA16 and KuA22 showed substantial homology only to some environmental clones. Both of these branched deeply in the Euryarchaeota, and may represent novel orders. Quantitative competitive PCR showed that KuA12 was the most abundant, accounting for ∼50% of the total archaeal rDNA copies detected. KuA1 and KuA16 also constituted significant proportions of the total archaeal rDNA copies (7 and 17%, respectively). These results suggest that limited species of novel archaea were enriched in the Oil Storage cavity. An estimate of specific methane production rates suggests that they were active methanogens.
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diversity abundance and activity of archaeal populations in Oil contaminated groundwater accumulated at the bottom of an underground crude Oil Storage cavity
Applied and Environmental Microbiology, 2002Co-Authors: Kazuya Watanabe, Yumiko Kodama, Natsuko Hamamura, Nobuo KakuAbstract:Fluorescence in situ hybridization has shown that cells labeled with an Archaea-specific probe (ARCH915) accounted for approximately 10% of the total cell count in Oil-contaminated groundwater accumulated at the bottom of an underground crude Oil Storage cavity. Although chemical analyses have revealed vigorous consumption of nitrate in cavity groundwater, the present study found that the methane production rate was higher than the nitrate consumption rate. To characterize the likely archaeal populations responsible for methane production in this system, fragments of 16S ribosomal DNA (rDNA) were amplified by PCR using eight different combinations of universal and Archaea-specific primers. Sequence analysis of 324 clones produced 23 different archaeal sequence types, all of which were affiliated with the kingdom Euryarchaeota. Among them, five sequence types (KuA1, KuA6, KuA12, KuA16, and KuA22) were obtained in abundance. KuA1 and KuA6 were closely related to the known methanogens Methanosaeta concilii (99% identical) and Methanomethylovorans hollandica (98%), respectively. Although no closely related organism was found for KuA12, it could be affiliated with the family Methanomicrobiaceae. KuA16 and KuA22 showed substantial homology only to some environmental clones. Both of these branched deeply in the Euryarchaeota, and may represent novel orders. Quantitative competitive PCR showed that KuA12 was the most abundant, accounting for ∼50% of the total archaeal rDNA copies detected. KuA1 and KuA16 also constituted significant proportions of the total archaeal rDNA copies (7 and 17%, respectively). These results suggest that limited species of novel archaea were enriched in the Oil Storage cavity. An estimate of specific methane production rates suggests that they were active methanogens.
Yumiko Kodama - One of the best experts on this subject based on the ideXlab platform.
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Yumiko Kodama and Kazuya Watanabe Correspondence
2016Co-Authors: Yumiko KodamaAbstract:facultatively anaerobic, chemolithoautotrophic, sulfur-oxidizing bacterium isolated from an underground crude-Oil Storage cavit
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sulfurospirillum cavolei sp nov a facultatively anaerobic sulfur reducing bacterium isolated from an underground crude Oil Storage cavity
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Yumiko Kodama, Le Thu Ha, Kazuya WatanabeAbstract:A novel facultatively anaerobic sulfur-reducing bacterium, designated strain Phe91T, was isolated from petroleum-contaminated groundwater in an underground crude Oil Storage cavity at Kuji in Iwate, Japan. Cells of strain Phe91T were slightly curved rods with single polar flagella. Optimum growth was observed at pH 7.0 and 30 °C. The novel strain utilized elemental sulfur, thiosulfate, sulfite, dithionite, arsenate, nitrate and DMSO as electron acceptors with lactate as an energy and carbon source, but nitrite was not utilized. Microaerophilic growth was also observed. Fumarate, pyruvate, lactate, malate, succinate, hydrogen (with acetate as a carbon source) and formate (with acetate) could serve as electron donors. Fumarate, pyruvate and malate were fermented. The DNA G+C content was 42.7 mol%. On the basis of 16S rRNA gene sequence phylogeny, strain Phe91T was affiliated with the genus Sulfurospirillum in the class Epsilonproteobacteria and was most closely related to Sulfurospirillum deleyianum (sequence similarity 97 %). However, the DNA–DNA hybridization value between strain Phe91T and S. deleyianum was only 14 %. Based on the physiological and phylogenetic data, Phe91T should be classified as a representative of a novel species in the genus Sulfurospirillum; the name Sulfurospirillum cavolei sp. nov. is proposed, with Phe91T (=JCM 13918T=DSM 18149T) as the type strain.
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sulfuricurvum kujiense gen nov sp nov a facultatively anaerobic chemolithoautotrophic sulfur oxidizing bacterium isolated from an underground crude Oil Storage cavity
International Journal of Systematic and Evolutionary Microbiology, 2004Co-Authors: Yumiko Kodama, Kazuya WatanabeAbstract:A facultatively anaerobic, chemolithoautotrophic, sulfur-oxidizing bacterium, strain YK-1T, was isolated from an underground crude-Oil Storage cavity at Kuji in Iwate, Japan. The cells were motile, curved rods and had a single polar flagellum. Optimum growth occurred in a low-strength salt medium at pH 7·0 and 25 °C. It utilized sulfide, elemental sulfur, thiosulfate and hydrogen as the electron donors and nitrate as the electron acceptor under anaerobic conditions, but it did not use nitrite. Oxygen also served as the electron acceptor under the microaerobic condition (O2 in the head space 1 %). It did not grow on sugars, organic acids or hydrocarbons as carbon and energy sources. The DNA G+C content of strain YK-1T was 45 mol%. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that its closest relative was Thiomicrospira denitrificans in the ‘Epsilonproteobacteria’, albeit with low homology (90 %). On the basis of physiological and phylogenetic data, strain YK-1T should be classified into a novel genus and species, for which the name Sulfuricurvum kujiense gen. nov., sp. nov. is proposed. The type strain is YK-1T (=JCM 11577T=MBIC 06352T=ATCC BAA-921T).
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sulfuricurvum kujiense gen nov sp nov a facultatively anaerobic chemolithoautotrophic sulfur oxidizing bacterium isolated from an underground crude Oil Storage cavity
International Journal of Systematic and Evolutionary Microbiology, 2004Co-Authors: Yumiko Kodama, Kazuya WatanabeAbstract:A facultatively anaerobic, chemolithoautotrophic, sulfur-oxidizing bacterium, strain YK-1(T), was isolated from an underground crude-Oil Storage cavity at Kuji in Iwate, Japan. The cells were motile, curved rods and had a single polar flagellum. Optimum growth occurred in a low-strength salt medium at pH 7.0 and 25 degrees C. It utilized sulfide, elemental sulfur, thiosulfate and hydrogen as the electron donors and nitrate as the electron acceptor under anaerobic conditions, but it did not use nitrite. Oxygen also served as the electron acceptor under the microaerobic condition (O(2) in the head space 1 %). It did not grow on sugars, organic acids or hydrocarbons as carbon and energy sources. The DNA G+C content of strain YK-1(T) was 45 mol%. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that its closest relative was Thiomicrospira denitrificans in the 'Epsilonproteobacteria', albeit with low homology (90 %). On the basis of physiological and phylogenetic data, strain YK-1(T) should be classified into a novel genus and species, for which the name Sulfuricurvum kujiense gen. nov., sp. nov. is proposed. The type strain is YK-1(T) (=JCM 11577(T)=MBIC 06352(T)=ATCC BAA-921(T)).
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diversity abundance and activity of archaeal populations in Oil contaminated groundwater accumulated at the bottom of an underground crude Oil Storage cavity
Applied and Environmental Microbiology, 2002Co-Authors: Kazuya Watanabe, Yumiko Kodama, Natsuko Hamamura, Nobuo KakuAbstract:Fluorescence in situ hybridization has shown that cells labeled with an Archaea-specific probe (ARCH915) accounted for approximately 10% of the total cell count in Oil-contaminated groundwater accumulated at the bottom of an underground crude Oil Storage cavity. Although chemical analyses have revealed vigorous consumption of nitrate in cavity groundwater, the present study found that the methane production rate was higher than the nitrate consumption rate. To characterize the likely archaeal populations responsible for methane production in this system, fragments of 16S ribosomal DNA (rDNA) were amplified by PCR using eight different combinations of universal and Archaea-specific primers. Sequence analysis of 324 clones produced 23 different archaeal sequence types, all of which were affiliated with the kingdom Euryarchaeota. Among them, five sequence types (KuA1, KuA6, KuA12, KuA16, and KuA22) were obtained in abundance. KuA1 and KuA6 were closely related to the known methanogens Methanosaeta concilii (99% identical) and Methanomethylovorans hollandica (98%), respectively. Although no closely related organism was found for KuA12, it could be affiliated with the family Methanomicrobiaceae. KuA16 and KuA22 showed substantial homology only to some environmental clones. Both of these branched deeply in the Euryarchaeota, and may represent novel orders. Quantitative competitive PCR showed that KuA12 was the most abundant, accounting for ∼50% of the total archaeal rDNA copies detected. KuA1 and KuA16 also constituted significant proportions of the total archaeal rDNA copies (7 and 17%, respectively). These results suggest that limited species of novel archaea were enriched in the Oil Storage cavity. An estimate of specific methane production rates suggests that they were active methanogens.
Shigeaki Harayama - One of the best experts on this subject based on the ideXlab platform.
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iron corroding methanogen isolated from a crude Oil Storage tank
Applied and Environmental Microbiology, 2010Co-Authors: Taku Uchiyama, Shigeaki Harayama, Kimio Ito, Koji Mori, Hirohito TsurumaruAbstract:Microbiologically influenced corrosion of steel in anaerobic environments has been attributed to hydrogenotrophic microorganisms. A sludge sample collected from the bottom plate of a crude-Oil Storage tank was used to inoculate a medium containing iron (Fe0) granules, which was then incubated anaerobically at 37°C under an N2-CO2 atmosphere to enrich for microorganisms capable of using iron as the sole source of electrons. A methanogen, designated strain KA1, was isolated from the enrichment culture. An analysis of its 16S rRNA gene sequence revealed that strain KA1 is a Methanococcus maripaludis strain. Strain KA1 produced methane and oxidized iron much faster than did the type strain of M. maripaludis, strain JJT, which produced methane at a rate expected from the abiotic H2 production rate from iron. Scanning electron micrographs of iron coupons that had been immersed in either a KA1 culture, a JJT culture, or an aseptic medium showed that only coupons from the KA1 culture had corroded substantially, and these were covered with crystalline deposits that consisted mainly of FeCO3.
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iron corroding methanogen isolated from a crude Oil Storage tank
Applied and Environmental Microbiology, 2010Co-Authors: Taku Uchiyama, Shigeaki Harayama, Kimio Ito, Koji Mori, Hirohito TsurumaruAbstract:Microbiologically influenced corrosion of steel in anaerobic environments has been attributed to hydrogenotrophic microorganisms. A sludge sample collected from the bottom plate of a crude-Oil Storage tank was used to inoculate a medium containing iron (Fe(0)) granules, which was then incubated anaerobically at 37 degrees C under an N(2)-CO(2) atmosphere to enrich for microorganisms capable of using iron as the sole source of electrons. A methanogen, designated strain KA1, was isolated from the enrichment culture. An analysis of its 16S rRNA gene sequence revealed that strain KA1 is a Methanococcus maripaludis strain. Strain KA1 produced methane and oxidized iron much faster than did the type strain of M. maripaludis, strain JJ(T), which produced methane at a rate expected from the abiotic H(2) production rate from iron. Scanning electron micrographs of iron coupons that had been immersed in either a KA1 culture, a JJ(T) culture, or an aseptic medium showed that only coupons from the KA1 culture had corroded substantially, and these were covered with crystalline deposits that consisted mainly of FeCO(3).
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molecular characterization of bacterial populations in petroleum contaminated groundwater discharged from underground crude Oil Storage cavities
Applied and Environmental Microbiology, 2000Co-Authors: Kazuya Watanabe, Kazuaki Syutsubo, Yumiko Kodama, Kanako Watanabe, Shigeaki HarayamaAbstract:Petroleum-contaminated groundwater discharged from underground crude Oil Storage cavities (cavity groundwater) harbored more than 106 microorganisms ml−1, a density 100 times higher than the densities in groundwater around the cavities (control groundwater). To characterize bacterial populations growing in the cavity groundwater, 46 PCR-amplified almost full-length 16S ribosomal DNA (rDNA) fragments were cloned and sequenced, and 28 different sequences were obtained. All of the sequences were affiliated with the Proteobacteria; 25 sequences (43 clones) were affiliated with the epsilon subclass, 2 were affiliated with the beta subclass, and 1 was affiliated with the delta subclass. Two major clusters (designated clusters 1 and 2) were found for the epsilon subclass proteobacterial clones; cluster 1 (25 clones) was most closely related to Thiomicrospira denitrificans (88% identical in nucleotide sequence), while cluster 2 (11 clones) was closely related to Arcobacterspp. Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified partial 16S rDNA fragments showed that one band was detected most strongly in cavity groundwater profiles independent of Storage Oil type and season. The sequence of this major band was identical to the sequences of most of the cluster 1 clones. Fluorescence in situ hybridization (FISH) indicated that the cluster 1 population accounted for 12 to 24% of the total bacterial population. This phylotype was not detected in the control groundwater by DGGE and FISH analyses. These results indicate that the novel members of the epsilon subclass of the Proteobacteria grow as major populations in the petroleum-contaminated cavity groundwater.
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molecular characterization of bacterial populations in petroleum contaminated groundwater discharged from underground crude Oil Storage cavities
Applied and Environmental Microbiology, 2000Co-Authors: Kazuya Watanabe, Kazuaki Syutsubo, Yumiko Kodama, Kanako Watanabe, Shigeaki HarayamaAbstract:water) harbored more than 10 6 microorganisms ml 21 , a density 100 times higher than the densities in groundwater around the cavities (control groundwater). To characterize bacterial populations growing in the cavity groundwater, 46 PCR-amplified almost full-length 16S ribosomal DNA (rDNA) fragments were cloned and sequenced, and 28 different sequences were obtained. All of the sequences were affiliated with the Proteobacteria; 25 sequences (43 clones) were affiliated with the epsilon subclass, 2 were affiliated with the beta subclass, and 1 was affiliated with the delta subclass. Two major clusters (designated clusters 1 and 2) were found for the epsilon subclass proteobacterial clones; cluster 1 (25 clones) was most closely related to Thiomicrospira denitrificans (88% identical in nucleotide sequence), while cluster 2 (11 clones) was closely related to Arcobacter spp. Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified partial 16S rDNA fragments showed that one band was detected most strongly in cavity groundwater profiles independent of Storage Oil type and season. The sequence of this major band was identical to the sequences of most of the cluster 1 clones. Fluorescence in situ hybridization (FISH) indicated that the cluster 1 population accounted for 12 to 24% of the total bacterial population. This phylotype was not detected in the control groundwater by DGGE and FISH analyses. These results indicate that the novel members of the epsilon subclass of the Proteobacteria grow as major populations in the petroleum-contaminated cavity groundwater. Underground cavities have been used for long-term Storage of crude Oil in several countries, such as Japan, Korea, and Norway, and are constructed in groundwater-rich rocky strata where high groundwater pressure confines the stored Oil in the cavities (Fig. 1). Consequently, groundwater migrates into and accumulates at the bottom of a cavity (cavity groundwater), and this cavity groundwater is discharged to maintain the Oil Storage capacity of the cavity. The discharged groundwater, which is contaminated with petroleum hydrocarbons, is then purified by using water clarifiers before release into the sea. Some of the purified water is also injected back into the rock surrounding the cavity to maintain the groundwater pressure. This flow of groundwater may establish a continuous culture in which microorganisms grow on petroleum hydrocarbons. The habitat in the cavity groundwater can be characterized by (i) immediate contact with a large quantity of crude Oil and (ii) an excess of electron donors (i.e., hydrocarbons) but a shortage of
Liping Qiao - One of the best experts on this subject based on the ideXlab platform.
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hydro geochemical analysis of the interplay between the groundwater host rock and water curtain system for an underground Oil Storage facility
Tunnelling and Underground Space Technology, 2018Co-Authors: Liping Qiao, Zhechao Wang, Y. Glais, A. HuangAbstract:Abstract Hydro-geochemical analysis was performed to investigate the interplay between the groundwater, host rock and water curtain system for the start-up of a pilot underground Oil Storage facility in China. 54 groundwater samples were collected and analyzed to obtain the characteristics of the hydro-geochemical environment evolution for the start-up period. The groundwater-rock mass interaction was studied with comparing the changes in the ion concentrations, pH values and total dissolved solid concentrations in the groundwater and a mineralogical analysis of the host rock. The groundwater-water curtain system interaction was identified using statistical analysis of the similarity in chemical contents in the groundwater samples. A mixing calculation was performed to evaluate the mixing ratios of the water curtain system, background water and Oil/vapor in the seepage water into the Storage caverns. It was concluded that calcium carbonate equilibrium is the predominant chemical reaction. The 54 groundwater samples could be classified into 5 clusters. Among the 5 clusters, there is one cluster showing that the tap water injected to the water curtain system is similar in chemical contents to those in the monitoring boreholes around the facility, which confirms the efficiency of the water curtain system for the start-up of the facility. It was found that the two dominant factors influencing the evolution of groundwater chemical content were host rock dissolution and groundwater seepage. Most of seepage water was originated from Oil/vapor and water curtain system while the percentage from the background water was almost zero in the start-up period of the facility.
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estimation of groundwater inflow into an underground Oil Storage facility in granite
Geomechanics and Engineering, 2017Co-Authors: Zhechao Wang, Sangki Kwon, Liping Bi, Liping Qiao, Liyuan YuAbstract:Estimation of groundwater inflow into underground opening is of critical importance for the design and construction of underground structures. Groundwater inflow into a pilot underground Storage facility in China was estimated using analytical equations, numerical modeling and field measurement. The applicability of analytical and numerical methods was examined by comparing the estimated and measured results. Field geological investigation indicated that in local scale the high groundwater inflows are associated with the appearance of open joints, fractured zone or dykes induced by shear and/or tensile tectonic stresses. It was found that 8 groundwater inflow spots with high inflow rates account for about 82% of the total rate for the 9 caverns. On the prediction of the magnitude of groundwater inflow rate, it was found that could both (Finite Element Method) FEM and (Discrete Element Method) DEM perform better than analytical equations, due to the fact that in analytical equations simplified assumptions were adopted. However, on the prediction of the spatial distribution estimation of groundwater inflow, both analytical and numerical methods failed to predict at the present state. Nevertheless, numerical simulations would prevail over analytical methods to predict the distribution if more details in the simulations were taken into consideration.
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risk assessment for stability and containment property of an underground Oil Storage facility in construction phase using fuzzy comprehensive evaluation method
ASCE-ASME Journal of Risk and Uncertainty in Engineering Systems Part A: Civil Engineering, 2016Co-Authors: Zhechao Wang, Liping Qiao, Shucai Li, Liping BiAbstract:AbstractRisk assessment for the construction of a pilot underground Oil Storage facility was performed in terms of its stability and containment property. The risk factors regulating the stability and containment property of the underground Storage cavern were identified from both design and construction aspects and also from geological conditions. Questionnaire inquiry was performed to assess the consequences and likelihoods of risk factors. Fuzzy comprehensive evaluation method was used to assess the roles of risk factors in regulating the stability and containment property of facilities. A five-step flowchart for fuzzy compression evaluation was proposed and adopted in this study. The risk values of risk factors for stability and containment property were obtained. It was found that the influences of geostress level, blast method, cavern shape, and rockbolt length and spacing are higher than those of the other factors for stability, whereas those of water curtain pressure and groundwater table are high...
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design and test aspects of a water curtain system for underground Oil Storage caverns in china
Tunnelling and Underground Space Technology, 2015Co-Authors: Zhechao Wang, Shucai Li, Liping QiaoAbstract:A water curtain system consists of many boreholes arranged in a systematic manner. To maintain tight seals when storing substances (e.g., crude Oil), water curtain systems are usually employed for underground Storage in hard rock. With the aid of these systems, stable seepage fields develop around Storage caverns, and hydrodynamic containment is achieved in the Storage caverns. In this paper, several issues regarding the design and testing of a water curtain system in underground Oil Storage caverns in China were investigated. Regarding the design of these systems, natural and artificial containments were compared, and the role of hydrogeology in the selection of containment was explored. A literature review on the geometrical parameters of these systems used in Storage caverns was performed and provided reference for the design of the proposed water curtain system. The influence of rock joint orientations on the arrangement of boreholes in the curtain system was addressed; it was concluded that the boreholes should be arranged perpendicularly to the dominant joints to obtain a system with good performance. Regarding the testing of these systems, a procedure used to evaluate the performance of the proposed water curtain system was developed. A method used to determine the interconnectivity of the system was also developed, and an improved test procedure was proposed based on the test results. A curtain system injection test was performed, and the groundwater flow rates into both the water curtain system and the Storage caverns were measured. It was shown that the groundwater flow rates were dominated by the groundwater flow in the rock joints and the areas where high groundwater flow rates around the cavern surfaces coincided with those where high groundwater flow rates appeared at water curtain boreholes.
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assessment of hydro mechanical behavior of a granite rock mass for a pilot underground crude Oil Storage facility in china
Rock Mechanics and Rock Engineering, 2015Co-Authors: Zhechao Wang, Shucai Li, Liping QiaoAbstract:The hydro-mechanical behavior of a pilot underground crude Oil Storage facility in a granite host rock in China was analyzed using the finite element method (FEM). Characterization of hydro-mechanical behavior of the rock mass was performed using laboratory test, field monitoring, back analysis of field measurements and permeability tests. FEM numerical analyses were used to assess the hydro-mechanical behavior of the granite to study several design and construction issues. The containment properties of the Storage facility were investigated without and with the water curtain system. Results showed that the stored Oil would leak into rock mass if a water curtain system is not provided, whereas the containment property of the facility will be maintained when a water curtain system is in place. On the influence of cavern excavation sequence, it was indicated that the excavation of the caverns from left to right is a better choice than right to left for the containment property of the facility. On the influence of permeable condition, it was found that the extent of plastic zones, horizontal convergence and crown settlement under permeable condition are lower than those under impermeable condition due to the different stress paths in the rock mass experienced during excavation.
Zhechao Wang - One of the best experts on this subject based on the ideXlab platform.
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hydro geochemical analysis of the interplay between the groundwater host rock and water curtain system for an underground Oil Storage facility
Tunnelling and Underground Space Technology, 2018Co-Authors: Liping Qiao, Zhechao Wang, Y. Glais, A. HuangAbstract:Abstract Hydro-geochemical analysis was performed to investigate the interplay between the groundwater, host rock and water curtain system for the start-up of a pilot underground Oil Storage facility in China. 54 groundwater samples were collected and analyzed to obtain the characteristics of the hydro-geochemical environment evolution for the start-up period. The groundwater-rock mass interaction was studied with comparing the changes in the ion concentrations, pH values and total dissolved solid concentrations in the groundwater and a mineralogical analysis of the host rock. The groundwater-water curtain system interaction was identified using statistical analysis of the similarity in chemical contents in the groundwater samples. A mixing calculation was performed to evaluate the mixing ratios of the water curtain system, background water and Oil/vapor in the seepage water into the Storage caverns. It was concluded that calcium carbonate equilibrium is the predominant chemical reaction. The 54 groundwater samples could be classified into 5 clusters. Among the 5 clusters, there is one cluster showing that the tap water injected to the water curtain system is similar in chemical contents to those in the monitoring boreholes around the facility, which confirms the efficiency of the water curtain system for the start-up of the facility. It was found that the two dominant factors influencing the evolution of groundwater chemical content were host rock dissolution and groundwater seepage. Most of seepage water was originated from Oil/vapor and water curtain system while the percentage from the background water was almost zero in the start-up period of the facility.
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estimation of groundwater inflow into an underground Oil Storage facility in granite
Geomechanics and Engineering, 2017Co-Authors: Zhechao Wang, Sangki Kwon, Liping Bi, Liping Qiao, Liyuan YuAbstract:Estimation of groundwater inflow into underground opening is of critical importance for the design and construction of underground structures. Groundwater inflow into a pilot underground Storage facility in China was estimated using analytical equations, numerical modeling and field measurement. The applicability of analytical and numerical methods was examined by comparing the estimated and measured results. Field geological investigation indicated that in local scale the high groundwater inflows are associated with the appearance of open joints, fractured zone or dykes induced by shear and/or tensile tectonic stresses. It was found that 8 groundwater inflow spots with high inflow rates account for about 82% of the total rate for the 9 caverns. On the prediction of the magnitude of groundwater inflow rate, it was found that could both (Finite Element Method) FEM and (Discrete Element Method) DEM perform better than analytical equations, due to the fact that in analytical equations simplified assumptions were adopted. However, on the prediction of the spatial distribution estimation of groundwater inflow, both analytical and numerical methods failed to predict at the present state. Nevertheless, numerical simulations would prevail over analytical methods to predict the distribution if more details in the simulations were taken into consideration.
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risk assessment for stability and containment property of an underground Oil Storage facility in construction phase using fuzzy comprehensive evaluation method
ASCE-ASME Journal of Risk and Uncertainty in Engineering Systems Part A: Civil Engineering, 2016Co-Authors: Zhechao Wang, Liping Qiao, Shucai Li, Liping BiAbstract:AbstractRisk assessment for the construction of a pilot underground Oil Storage facility was performed in terms of its stability and containment property. The risk factors regulating the stability and containment property of the underground Storage cavern were identified from both design and construction aspects and also from geological conditions. Questionnaire inquiry was performed to assess the consequences and likelihoods of risk factors. Fuzzy comprehensive evaluation method was used to assess the roles of risk factors in regulating the stability and containment property of facilities. A five-step flowchart for fuzzy compression evaluation was proposed and adopted in this study. The risk values of risk factors for stability and containment property were obtained. It was found that the influences of geostress level, blast method, cavern shape, and rockbolt length and spacing are higher than those of the other factors for stability, whereas those of water curtain pressure and groundwater table are high...
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design and test aspects of a water curtain system for underground Oil Storage caverns in china
Tunnelling and Underground Space Technology, 2015Co-Authors: Zhechao Wang, Shucai Li, Liping QiaoAbstract:A water curtain system consists of many boreholes arranged in a systematic manner. To maintain tight seals when storing substances (e.g., crude Oil), water curtain systems are usually employed for underground Storage in hard rock. With the aid of these systems, stable seepage fields develop around Storage caverns, and hydrodynamic containment is achieved in the Storage caverns. In this paper, several issues regarding the design and testing of a water curtain system in underground Oil Storage caverns in China were investigated. Regarding the design of these systems, natural and artificial containments were compared, and the role of hydrogeology in the selection of containment was explored. A literature review on the geometrical parameters of these systems used in Storage caverns was performed and provided reference for the design of the proposed water curtain system. The influence of rock joint orientations on the arrangement of boreholes in the curtain system was addressed; it was concluded that the boreholes should be arranged perpendicularly to the dominant joints to obtain a system with good performance. Regarding the testing of these systems, a procedure used to evaluate the performance of the proposed water curtain system was developed. A method used to determine the interconnectivity of the system was also developed, and an improved test procedure was proposed based on the test results. A curtain system injection test was performed, and the groundwater flow rates into both the water curtain system and the Storage caverns were measured. It was shown that the groundwater flow rates were dominated by the groundwater flow in the rock joints and the areas where high groundwater flow rates around the cavern surfaces coincided with those where high groundwater flow rates appeared at water curtain boreholes.
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assessment of hydro mechanical behavior of a granite rock mass for a pilot underground crude Oil Storage facility in china
Rock Mechanics and Rock Engineering, 2015Co-Authors: Zhechao Wang, Shucai Li, Liping QiaoAbstract:The hydro-mechanical behavior of a pilot underground crude Oil Storage facility in a granite host rock in China was analyzed using the finite element method (FEM). Characterization of hydro-mechanical behavior of the rock mass was performed using laboratory test, field monitoring, back analysis of field measurements and permeability tests. FEM numerical analyses were used to assess the hydro-mechanical behavior of the granite to study several design and construction issues. The containment properties of the Storage facility were investigated without and with the water curtain system. Results showed that the stored Oil would leak into rock mass if a water curtain system is not provided, whereas the containment property of the facility will be maintained when a water curtain system is in place. On the influence of cavern excavation sequence, it was indicated that the excavation of the caverns from left to right is a better choice than right to left for the containment property of the facility. On the influence of permeable condition, it was found that the extent of plastic zones, horizontal convergence and crown settlement under permeable condition are lower than those under impermeable condition due to the different stress paths in the rock mass experienced during excavation.
