Olfactometers

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Didier Rochat - One of the best experts on this subject based on the ideXlab platform.

  • Synthetic Co-Attractants of the Aggregation Pheromone of the Date Palm Root Borer Oryctes agamemnon
    Journal of Chemical Ecology, 2017
    Co-Authors: Narjes Hasni, Centina Pinier, Cheraief Imed, Monêem Ouhichi, Imen Saïd, Philippe Couzi, Brigitte Frerot, Brahim Chermiti, Didier Rochat
    Abstract:

    Laboratory and field investigations to identify and evaluate plant co-attractants of the aggregation pheromone of the date palm pest Oryctes agamemnon are reported. Volatiles emitted by freshly cut palm core and palm core with feeding males, were collected, analyzed by gas chromatography coupled to mass spectrometry and evaluated in Olfactometers alone or combined with synthetic pheromone. A collection of palm odor without male effluvia was attractive alone and enhanced attraction to synthetic pheromone in an olfactometer similar to that to a collection of palm odor emitted with feeding males and containing natural pheromone. Behavioral responses to collections of palm volatiles were correlated to the amount of volatiles material in them. Enhancement of the attractiveness of the pheromone was not correlated to chemicals specific to beetle feeding. The chemicals common to the active collections extracts were benzoate esters, mostly ethyl benzoate, anisole derivatives and sesquiterpenes. Blends of the most abundant components of the extracts were evaluated for enhancement of the attractiveness of pheromone (1 μg) in Olfactometers at 1 or 10 μg doses. The mixtures were further evaluated by field trapping in Tunisia at 3–10 mg/day using reference (6 mg/day) or experimental pheromone formulations. A mixture of ethyl benzoate, 4-methylanisole and farnesol (1:1:1 w/w at 6.5 mg/day) enhanced captures in pheromone baited traps in 2014 and 2015 and this mixture was as active as the natural palm bait. The practical prospect of the result for the management for O. agamemnon, and other palm beetles is discussed.

  • Synthetic Co-Attractants of the Aggregation Pheromone of the Date Palm Root Borer Oryctes agamemnon
    Journal of Chemical Ecology, 2017
    Co-Authors: Narjes Hasni, Centina Pinier, Cheraief Imed, Monêem Ouhichi, Imen Saïd, Philippe Couzi, Brigitte Frerot, Brahim Chermiti, Didier Rochat
    Abstract:

    Laboratory and field investigations to identify and evaluate plant co-attractants of the aggregation pheromone of the date palm pest Oryctes agamemnon are reported. Volatiles emitted by freshly cut palm core and palm core with feeding males, were collected, analyzed by gas chromatography coupled to mass spectrometry and evaluated in Olfactometers alone or combined with synthetic pheromone. A collection of palm odor without male effluvia was attractive alone and enhanced attraction to synthetic pheromone in an olfactometer similar to that to a collection of palm odor emitted with feeding males and containing natural pheromone. Behavioral responses to collections of palm volatiles were correlated to the amount of volatiles material in them. Enhancement of the attractiveness of the pheromone was not correlated to chemicals specific to beetle feeding. The chemicals common to the active collections extracts were benzoate esters, mostly ethyl benzoate, anisole derivatives and sesquiterpenes. Blends of the most abundant components of the extracts were evaluated for enhancement of the attractiveness of pheromone (1 μg) in Olfactometers at 1 or 10 μg doses. The mixtures were further evaluated by field trapping in Tunisia at 3–10 mg/day using reference (6 mg/day) or experimental pheromone formulations. A mixture of ethyl benzoate, 4-methylanisole and farnesol (1:1:1 w/w at 6.5 mg/day) enhanced captures in pheromone baited traps in 2014 and 2015 and this mixture was as active as the natural palm bait. The practical prospect of the result for the management for O. agamemnon, and other palm beetles is discussed.

Michael P Parrella - One of the best experts on this subject based on the ideXlab platform.

  • quantitative assessment of insect olfactometer performance by experimental flow analysis
    Biological Control, 1996
    Co-Authors: Michael P Parrella, Kevin M. Heinz, D K Giles
    Abstract:

    Abstract Biocontrol organisms respond to olfactory cues with behavior which can affect their efficacy as pest control agents. Investigations into olfactory stimuli and responses often use four-armed Olfactometers for laboratory experiments. Performance evaluations of the Olfactometers have generally been qualitative and based on visual assessment. A quantitative technique for characterizing the air flow and gas mixing in a four-armed olfactometer was developed. Ethylene gas was injected into the flow stream for each channel of the olfactometer. Samples of air at various locations in the olfactometer chamber were drawn through hypodermic probes and the concentration of ethylene in the samples were determined by gas chromatography. Flow boundaries between adjacent channels were not distinct and mixing between channels was observed, although concentration spatial gradients were high in the boundary regions. Mixing increased as flow neared a central outlet. Gross assessments of organism behavior in Olfactometers appear valid in spite of flow mixing.

Richard J Stevenson - One of the best experts on this subject based on the ideXlab platform.

  • a comparison of sniff bottle staircase and olfactometer based threshold tests
    Behavior Research Methods, 2013
    Co-Authors: James Hayes, Anthony L Jinks, Richard J Stevenson
    Abstract:

    Olfactometers have been gaining popularity as research tools, but they have yet to replace established testing procedures in a variety of laboratory and clinical settings, including absolute threshold tests. In this research, we designed and operated a simple olfactometer with which to assess threshold. To do this, we used a method-of-adjustment test that was compared to the three-alternative forced choice ascending sniff bottle staircase method, which is currently a standard threshold test procedure. We found that the olfactometer threshold test correlated highly with the staircase method, and that it possessed suitable test–retest reliability. The advantages of the olfactometer threshold test include faster test time and reduced cleaning and reassembly demands. Future use of the olfactometer in olfactory identification and/or detection thresholds amongst odors is also outlined.

Narjes Hasni - One of the best experts on this subject based on the ideXlab platform.

  • Synthetic Co-Attractants of the Aggregation Pheromone of the Date Palm Root Borer Oryctes agamemnon
    Journal of Chemical Ecology, 2017
    Co-Authors: Narjes Hasni, Centina Pinier, Cheraief Imed, Monêem Ouhichi, Imen Saïd, Philippe Couzi, Brigitte Frerot, Brahim Chermiti, Didier Rochat
    Abstract:

    Laboratory and field investigations to identify and evaluate plant co-attractants of the aggregation pheromone of the date palm pest Oryctes agamemnon are reported. Volatiles emitted by freshly cut palm core and palm core with feeding males, were collected, analyzed by gas chromatography coupled to mass spectrometry and evaluated in Olfactometers alone or combined with synthetic pheromone. A collection of palm odor without male effluvia was attractive alone and enhanced attraction to synthetic pheromone in an olfactometer similar to that to a collection of palm odor emitted with feeding males and containing natural pheromone. Behavioral responses to collections of palm volatiles were correlated to the amount of volatiles material in them. Enhancement of the attractiveness of the pheromone was not correlated to chemicals specific to beetle feeding. The chemicals common to the active collections extracts were benzoate esters, mostly ethyl benzoate, anisole derivatives and sesquiterpenes. Blends of the most abundant components of the extracts were evaluated for enhancement of the attractiveness of pheromone (1 μg) in Olfactometers at 1 or 10 μg doses. The mixtures were further evaluated by field trapping in Tunisia at 3–10 mg/day using reference (6 mg/day) or experimental pheromone formulations. A mixture of ethyl benzoate, 4-methylanisole and farnesol (1:1:1 w/w at 6.5 mg/day) enhanced captures in pheromone baited traps in 2014 and 2015 and this mixture was as active as the natural palm bait. The practical prospect of the result for the management for O. agamemnon, and other palm beetles is discussed.

  • Synthetic Co-Attractants of the Aggregation Pheromone of the Date Palm Root Borer Oryctes agamemnon
    Journal of Chemical Ecology, 2017
    Co-Authors: Narjes Hasni, Centina Pinier, Cheraief Imed, Monêem Ouhichi, Imen Saïd, Philippe Couzi, Brigitte Frerot, Brahim Chermiti, Didier Rochat
    Abstract:

    Laboratory and field investigations to identify and evaluate plant co-attractants of the aggregation pheromone of the date palm pest Oryctes agamemnon are reported. Volatiles emitted by freshly cut palm core and palm core with feeding males, were collected, analyzed by gas chromatography coupled to mass spectrometry and evaluated in Olfactometers alone or combined with synthetic pheromone. A collection of palm odor without male effluvia was attractive alone and enhanced attraction to synthetic pheromone in an olfactometer similar to that to a collection of palm odor emitted with feeding males and containing natural pheromone. Behavioral responses to collections of palm volatiles were correlated to the amount of volatiles material in them. Enhancement of the attractiveness of the pheromone was not correlated to chemicals specific to beetle feeding. The chemicals common to the active collections extracts were benzoate esters, mostly ethyl benzoate, anisole derivatives and sesquiterpenes. Blends of the most abundant components of the extracts were evaluated for enhancement of the attractiveness of pheromone (1 μg) in Olfactometers at 1 or 10 μg doses. The mixtures were further evaluated by field trapping in Tunisia at 3–10 mg/day using reference (6 mg/day) or experimental pheromone formulations. A mixture of ethyl benzoate, 4-methylanisole and farnesol (1:1:1 w/w at 6.5 mg/day) enhanced captures in pheromone baited traps in 2014 and 2015 and this mixture was as active as the natural palm bait. The practical prospect of the result for the management for O. agamemnon, and other palm beetles is discussed.

Philip E Otienoburu - One of the best experts on this subject based on the ideXlab platform.

  • orientation of anopheles gambiae diptera culicidae to plant host volatiles in a novel diffusion cage olfactometer
    Journal of Medical Entomology, 2016
    Co-Authors: Philip E Otienoburu, Mahmood Reza Nikbakhtzadeh, Woodbridge A Foster
    Abstract:

    A novel diffusion-cage olfactometer tested the responses of Anopheles gambiae Giles to plant volatiles. Green-leaf volatiles are often released from cut or injured plant tissue and may alter the headspace of plants used in olfactometer assays. The diffusion-cage olfactometer is designed for use with whole, intact plants, hence giving a more realistic behavioral assay. Its simple plastic construction, ease of assembly, and accommodation to whole plants makes it a useful tool for measuring mosquito orientation to plant volatiles within large enclosures. We compared its performance to that of the more commonly used T-tube wind-tunnel olfactometer, by testing the orientation of mosquitoes to volatiles of a few prevalent plants of eastern Africa reportedly utilized by An. gambiae for sugar: Parthenium hysterophorus (Asteraceae), Ricinus communis (Euphorbiaceae), Lantana camara (Verbenaceae), and Senna occidentalis (Fabaceae). Results indicate that the diffusion-cage olfactometer is an effective alternative to conventional wind-tunnel Olfactometers, to test mosquito orientation to plant volatiles under seminatural conditions.

  • orientation of anopheles gambiae diptera culicidae to plant host volatiles in a novel diffusion cage olfactometer
    Journal of Medical Entomology, 2016
    Co-Authors: Philip E Otienoburu, Mahmood Reza Nikbakhtzadeh, Woodbridge A Foster
    Abstract:

    A novel diffusion-cage olfactometer tested the responses of Anopheles gambiae Giles to plant volatiles. Green-leaf volatiles are often released from cut or injured plant tissue and may alter the headspace of plants used in olfactometer assays. The diffusion-cage olfactometer is designed for use with whole, intact plants, hence giving a more realistic behavioral assay. Its simple plastic construction, ease of assembly, and accommodation to whole plants makes it a useful tool for measuring mosquito orientation to plant volatiles within large enclosures. We compared its performance to that of the more commonly used T-tube wind-tunnel olfactometer, by testing the orientation of mosquitoes to volatiles of a few prevalent plants of eastern Africa reportedly utilized by An. gambiae for sugar: Parthenium hysterophorus (Asteraceae), Ricinus communis (Euphorbiaceae), Lantana camara (Verbenaceae), and Senna occidentalis (Fabaceae). Results indicate that the diffusion-cage olfactometer is an effective alternative to conventional wind-tunnel Olfactometers, to test mosquito orientation to plant volatiles under seminatural conditions.