The Experts below are selected from a list of 5931 Experts worldwide ranked by ideXlab platform
Julian A Guttman - One of the best experts on this subject based on the ideXlab platform.
-
Figure S3. Palladin Recruitment at L. monocytogenes Requires ActA.
2018Co-Authors: Arandeep Dhanda, Wayne A Vogl, Sharifah E Albraiki, Carol A Otey, Moriah R Beck, Julian A GuttmanAbstract:HeLa cells were infected with L. monocytogenes strain ΔactA for 6 hours, fixed and stained with a mouse monoclonal palladin targeting antibody (green), DAPI (blue) to visualize DNA and Alexa594-phalloidin (red) to visualize actin. Palladin is absent from the surface of intracellular bacteria (boxed regions). Insets (enlargement of boxed regions) points to an individual bacterium (Open Arrowhead). Scale bar, 10 µm and 2 µm (insets).
-
Supplemental Figure 2. CD147 is enriched at sites of L. monocytogenes cell-to-cell spreading in Caco-2 cells.
2018Co-Authors: Arandeep Dhanda, Katarina Lulic, Robert Chiu, Michael Bukrinsky, Julian A GuttmanAbstract:Caco-2 cells were infected with wild-type L. monocytogenes for 5 hours then fixed and stained with mouse monoclonal CD147-targeting antibodies (green), Alexa594-phalloidin (red) to visualize F-actin and DAPI (blue) to visualize DNA. CD147 was enriched at the plasma membrane surrounding membrane protrusions. The Open Arrowhead indicates the L. monocytogenes bacterium (blue). The solid Arrowhead points to CD147 at the membrane protrusion. Scale bar is 5 μm.
-
Supplemental Figure 1. Primary antibody and GFP vector controls do not localize to L. monocytogenes membrane protrusions or invaginations.
2018Co-Authors: Arandeep Dhanda, Katarina Lulic, Robert Chiu, Michael Bukrinsky, Julian A GuttmanAbstract:(A) HeLa cells were infected with wild-type L. monocytogenes for 6 hours. Samples were fixed then stained with normal mouse IgG antibody (green), Alexa594-phalloidin (red) to visualize F-actin and DAPI (blue) to visualize DNA. The control IgG is not enriched at the plasma membrane surrounding membrane protrusions (top) or at invaginations (bottom). Open Arrowheads indicate L. monocytogenes bacteria (blue) while solid Arrowheads point to protruding bacteria (red). Scale bar is 5 μm. (B) HeLa cells transfected with GFP alone (green) were infected with wild-type L. monocytogenes for 6 hours. They were then fixed and stained with Alexa594-phalloidin (red) to visualize F-actin and DAPI (blue) to visualize DNA. GFP alone is not enriched at the plasma membrane surrounding the membrane protrusions (solid Arrowhead) or at invaginations (Open Arrowhead). Scale bar is 5 μm
-
Figure S2. L. monocytogenes Invasion and Listeriopods Are Unaffected by Palladin Depletion.
2018Co-Authors: Arandeep Dhanda, Wayne A Vogl, Sharifah E Albraiki, Carol A Otey, Moriah R Beck, Julian A GuttmanAbstract:HeLa cells were treated with non-targeting control (Ctrl) or palladin-targeted (KD) siRNA sequences and infected with wild type L. monocytogenes. (A) HeLa cells were fixed following 3 hour infections and stained with a mouse monoclonal palladin targeting antibody (green), DAPI (blue) to visualize DNA and Alexa594-phalloidin (red) to visualize actin. Bacterial internalization efficiency was analyzed by counting and comparing the number of total internalized bacteria in Ctrl and KD (no detectable palladin observed by immunofluorescence microscopy) cells. In 3 independent experiments, 951 bacteria were counted in a total of 10 Ctrl cells and 822 bacteria were counted in a total of 10 KD cells; for each experiment all counts were normalized to the Ctrl counts. Data depicts percentage of bacteria invasion compared to Ctrl cells. (B) Palladin-depleted cells were fixed following 6 hour infections and stained with a mouse monoclonal palladin targeting antibody (green), DAPI (blue) to visualize DNA and Alexa594-phalloidin (red) to visualize actin. Listeriopod formation (arrow) is unaffected by palladin depletion. Insets depict enlargement of region indicated by arrow and shows listeriopod of interest (Open Arrowhead). Scale bars, 5 µm and 1 µm (insets).
-
Supplemental Figure 3. Palladin Recruitment at L. monocytogenes Requires ActA.m
2017Co-Authors: Arandeep Dhanda, Wayne A Vogl, Sharifah E Albraiki, Carol A Otey, Moriah R Beck, Julian A GuttmanAbstract:HeLa cells were infected with L. monocytogenes strain ΔactA for 6 hours, fixed and stained with a mouse monoclonal palladin targeting antibody (green), DAPI (blue) to visualize DNA and Alexa594-phalloidin (red) to visualize actin. Palladin is absent from the surface of intracellular bacteria (boxed regions). Insets (enlargement of boxed regions) points to an individual bacterium (Open Arrowhead). Scale bar, 10 µm and 2 µm (insets)
Arandeep Dhanda - One of the best experts on this subject based on the ideXlab platform.
-
Figure S3. Palladin Recruitment at L. monocytogenes Requires ActA.
2018Co-Authors: Arandeep Dhanda, Wayne A Vogl, Sharifah E Albraiki, Carol A Otey, Moriah R Beck, Julian A GuttmanAbstract:HeLa cells were infected with L. monocytogenes strain ΔactA for 6 hours, fixed and stained with a mouse monoclonal palladin targeting antibody (green), DAPI (blue) to visualize DNA and Alexa594-phalloidin (red) to visualize actin. Palladin is absent from the surface of intracellular bacteria (boxed regions). Insets (enlargement of boxed regions) points to an individual bacterium (Open Arrowhead). Scale bar, 10 µm and 2 µm (insets).
-
Supplemental Figure 2. CD147 is enriched at sites of L. monocytogenes cell-to-cell spreading in Caco-2 cells.
2018Co-Authors: Arandeep Dhanda, Katarina Lulic, Robert Chiu, Michael Bukrinsky, Julian A GuttmanAbstract:Caco-2 cells were infected with wild-type L. monocytogenes for 5 hours then fixed and stained with mouse monoclonal CD147-targeting antibodies (green), Alexa594-phalloidin (red) to visualize F-actin and DAPI (blue) to visualize DNA. CD147 was enriched at the plasma membrane surrounding membrane protrusions. The Open Arrowhead indicates the L. monocytogenes bacterium (blue). The solid Arrowhead points to CD147 at the membrane protrusion. Scale bar is 5 μm.
-
Supplemental Figure 1. Primary antibody and GFP vector controls do not localize to L. monocytogenes membrane protrusions or invaginations.
2018Co-Authors: Arandeep Dhanda, Katarina Lulic, Robert Chiu, Michael Bukrinsky, Julian A GuttmanAbstract:(A) HeLa cells were infected with wild-type L. monocytogenes for 6 hours. Samples were fixed then stained with normal mouse IgG antibody (green), Alexa594-phalloidin (red) to visualize F-actin and DAPI (blue) to visualize DNA. The control IgG is not enriched at the plasma membrane surrounding membrane protrusions (top) or at invaginations (bottom). Open Arrowheads indicate L. monocytogenes bacteria (blue) while solid Arrowheads point to protruding bacteria (red). Scale bar is 5 μm. (B) HeLa cells transfected with GFP alone (green) were infected with wild-type L. monocytogenes for 6 hours. They were then fixed and stained with Alexa594-phalloidin (red) to visualize F-actin and DAPI (blue) to visualize DNA. GFP alone is not enriched at the plasma membrane surrounding the membrane protrusions (solid Arrowhead) or at invaginations (Open Arrowhead). Scale bar is 5 μm
-
Figure S2. L. monocytogenes Invasion and Listeriopods Are Unaffected by Palladin Depletion.
2018Co-Authors: Arandeep Dhanda, Wayne A Vogl, Sharifah E Albraiki, Carol A Otey, Moriah R Beck, Julian A GuttmanAbstract:HeLa cells were treated with non-targeting control (Ctrl) or palladin-targeted (KD) siRNA sequences and infected with wild type L. monocytogenes. (A) HeLa cells were fixed following 3 hour infections and stained with a mouse monoclonal palladin targeting antibody (green), DAPI (blue) to visualize DNA and Alexa594-phalloidin (red) to visualize actin. Bacterial internalization efficiency was analyzed by counting and comparing the number of total internalized bacteria in Ctrl and KD (no detectable palladin observed by immunofluorescence microscopy) cells. In 3 independent experiments, 951 bacteria were counted in a total of 10 Ctrl cells and 822 bacteria were counted in a total of 10 KD cells; for each experiment all counts were normalized to the Ctrl counts. Data depicts percentage of bacteria invasion compared to Ctrl cells. (B) Palladin-depleted cells were fixed following 6 hour infections and stained with a mouse monoclonal palladin targeting antibody (green), DAPI (blue) to visualize DNA and Alexa594-phalloidin (red) to visualize actin. Listeriopod formation (arrow) is unaffected by palladin depletion. Insets depict enlargement of region indicated by arrow and shows listeriopod of interest (Open Arrowhead). Scale bars, 5 µm and 1 µm (insets).
-
Supplemental Figure 3. Palladin Recruitment at L. monocytogenes Requires ActA.m
2017Co-Authors: Arandeep Dhanda, Wayne A Vogl, Sharifah E Albraiki, Carol A Otey, Moriah R Beck, Julian A GuttmanAbstract:HeLa cells were infected with L. monocytogenes strain ΔactA for 6 hours, fixed and stained with a mouse monoclonal palladin targeting antibody (green), DAPI (blue) to visualize DNA and Alexa594-phalloidin (red) to visualize actin. Palladin is absent from the surface of intracellular bacteria (boxed regions). Insets (enlargement of boxed regions) points to an individual bacterium (Open Arrowhead). Scale bar, 10 µm and 2 µm (insets)
Jerker Delsing - One of the best experts on this subject based on the ideXlab platform.
-
Service Oriented Architecture enabling the 4th Generation of District Heating
Energy Procedia, 2017Co-Authors: Jan Van Deventer, Hasan Derhamy, Khalid Tourkey Atta, Jerker DelsingAbstract:Abstract The 4th Generation of District Heating (4GDH) is a complex agglomeration of heat providers, distributors, and consumers that must be automatically, continuously managed and coordinated. It is a complex system of systems; a definition which we align to Maier's architecting principles for systems-of-systems as collaborative systems. Wrapped in the idea of system of systems is the reality that the 4GDH systems’ descriptions and specifications are not currently all known. Nonetheless, the transition into the 4GDH is actual. We propose the use of two frameworks to secure a smooth metamorphosis and assure systems’ operation, maintenance, and evolution. The two frameworks are the Arrowhead Framework and the OPTi Framework. The first one enables system integration through Service Oriented Architecture (SOA) and the second one offers the overall system optimization with respect to all stakeholders. This paper uses the Model Based Systems Engineering (MBSE) tool SysML to model a district heating complex's structures and behaviors from the concept level down to the sensors and actuators within a district heating substation where we apply the SOA technology based on the Open Arrowhead Framework. We focus on the Arrowhead Framework's core services, i.e. Service Registry, Authorization and Orchestration to clearly describe the interactions between the different service providers and consumers. Going back up from the sensors to the systems, it is clear that SOA is the architecture that will empower the 4GDH.
Sha Sun - One of the best experts on this subject based on the ideXlab platform.
-
Jpx transgenes induce Xist expression in mESCs using both trans and cis mechanisms.
2018Co-Authors: Sarah Carmona, Benjamin Lin, Tristan Chou, Katti Arroyo, Sha SunAbstract:(A) Map of the X-inactivation center (Xic) with 90kb Tg(Jpx) and 120kb Tg(Jpx, Xist) transgenes and the probes used for Fluorescence in situ Hybridization (FISH). (B) Combined RNA-DNA FISH for Xist (green, FITC) and Xpct (red, Cy3) on female mESC at differentiation days 0, 2, and 4. Top: Control female ESCs transfected with Tg(pSKYneo+), a plasmid that does not contain X-chromosome sequence but provides the same neomycin resistance as Tg(Jpx, Xist); bottom: Tg(Jpx, Xist) transgenic female mESC line #7, which has a single-copy Tg(Jpx, Xist) integrated in an autosome; right: schematic of Xist expression at Day 4. Green wavy line, Xist RNA; Red dot, Xpct (X-Chr.) DNA locus; Open Arrowhead: Tg(Jpx, Xist) transgenic site. Scale bar: 2μm. (C) Xist cloud frequency throughout differentiation. **, P
-
Jpx utilizes a trans mechanism to activate Xist expression in Tg(Jpx) mice.
2018Co-Authors: Sarah Carmona, Benjamin Lin, Tristan Chou, Katti Arroyo, Sha SunAbstract:(A) RNA FISH (left column) and corresponding DNA FISH (two columns on the right) in wildtype and Tg(Jpx) transgenic mEFs. Representative images shown of ectopic expression patterns observed in cells. Probes are described in Fig 1A. For RNA: Jpx (red, Cy3) and Xist (green, FITC). For DNA: Jpx+Xist (red, Cy3) and Rnf12 (green, FITC). Right column: DNA FISH with two probes to distinguish the endogenous X chromosomal locus (overlapping red and green) from the transgenic insertion site (red only). Closed Arrowhead: endogenous RNA transcripts (RNA FISH) and the endogenous X chromosomal loci (DNA FISH). Open Arrowhead: transgenic RNA transcripts and the transgenic integration site. Scale bar: 5μm. (B) Percentage of cells with Jpx or Xist expression categorized by the number of RNA clouds detected. (C) Percentage of endogenous versus transgenic Jpx clouds counted in Tg(Jpx) mEFs.
-
Jpx activates Xist expression using both cis and trans mechanisms in Tg(Jpx, Xist) mice.
2018Co-Authors: Sarah Carmona, Benjamin Lin, Tristan Chou, Katti Arroyo, Sha SunAbstract:(A) RNA FISH (left column) and corresponding DNA FISH (two columns on the right) in transgenic Tg(Jpx, Xist) mEFs. Representative images shown of ectopic expression patterns observed in cells. Probes are described in Fig 1A. For RNA: Jpx (red, Cy3) and Xist (green, FITC); for DNA: Jpx+Xist (red, Cy3) and Rnf12 (green, FITC). Right column: DNA FISH with two probes to distinguish the endogenous X chromosomal locus (overlapping red and green) from the transgenic insertion site (red only). Closed Arrowhead: endogenous RNA transcripts (RNA FISH) and the endogenous X chromosomal loci (DNA FISH). Open Arrowhead: transgenic RNA transcripts and the transgenic integration site. Scale bar: 5μm. (B) Percentage of cells with Jpx or Xist expression categorized by number of RNA clouds detected. (C) Percentage of endogenous versus transgenic RNA clouds for Jpx and Xist in Tg(Jpx, Xist) mEFs.
-
Ectopic Xist expression in transgenic female and male early embryos.
2018Co-Authors: Sarah Carmona, Benjamin Lin, Tristan Chou, Katti Arroyo, Sha SunAbstract:(A, B) RNA FISH (left column) and corresponding DNA FISH (right column) in wildtype, transgenic Tg(Jpx) (A), and transgenic Tg(Jpx, Xist) embryos (B), extracted at embryonic day 7.5 (E7.5). Representative images shown of ectopic expression patterns observed in cells. Probes are described in Fig 1A. For RNA: Jpx (red, Cy3) and Xist (green, FITC); for DNA: Jpx+Xist (red, Cy3) and Rnf12 (green, FITC). DNA FISH with two probes distinguishes the endogenous X chromosomal locus (overlapping red and green) from the transgenic insertion site (red only). Closed Arrowhead: endogenous RNA transcripts (RNA FISH) and the endogenous X chromosomal loci (DNA FISH). Open Arrowhead: transgenic RNA transcripts and the transgenic integration site. Scale bar: 5μm. (C) Percentage of cells with Jpx or Xist expression categorized by number of RNA clouds detected. (D) Percentage of endogenous versus transgenic RNA clouds for Jpx and Xist in Tg(Jpx) and Tg(Jpx, Xist) embryos. Number of E7.5 embryos and quantification are included in S3A Fig.
Jan Van Deventer - One of the best experts on this subject based on the ideXlab platform.
-
Service Oriented Architecture enabling the 4th Generation of District Heating
Energy Procedia, 2017Co-Authors: Jan Van Deventer, Hasan Derhamy, Khalid Tourkey Atta, Jerker DelsingAbstract:Abstract The 4th Generation of District Heating (4GDH) is a complex agglomeration of heat providers, distributors, and consumers that must be automatically, continuously managed and coordinated. It is a complex system of systems; a definition which we align to Maier's architecting principles for systems-of-systems as collaborative systems. Wrapped in the idea of system of systems is the reality that the 4GDH systems’ descriptions and specifications are not currently all known. Nonetheless, the transition into the 4GDH is actual. We propose the use of two frameworks to secure a smooth metamorphosis and assure systems’ operation, maintenance, and evolution. The two frameworks are the Arrowhead Framework and the OPTi Framework. The first one enables system integration through Service Oriented Architecture (SOA) and the second one offers the overall system optimization with respect to all stakeholders. This paper uses the Model Based Systems Engineering (MBSE) tool SysML to model a district heating complex's structures and behaviors from the concept level down to the sensors and actuators within a district heating substation where we apply the SOA technology based on the Open Arrowhead Framework. We focus on the Arrowhead Framework's core services, i.e. Service Registry, Authorization and Orchestration to clearly describe the interactions between the different service providers and consumers. Going back up from the sensors to the systems, it is clear that SOA is the architecture that will empower the 4GDH.
