The Experts below are selected from a list of 6813 Experts worldwide ranked by ideXlab platform
Junfeng Wang - One of the best experts on this subject based on the ideXlab platform.
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biomass productivity of scenedesmus dimorphus chlorophyceae was improved by using an Open Pond photobioreactor hybrid system
European Journal of Phycology, 2019Co-Authors: Wen Liu, Yu Chen, Junfeng Wang, Tianzhong LiuAbstract:The industrialization of microalgae-based biofuel production has been hampered by low biomass productivity of conventional Open Ponds. In this research, a hybrid cultivation system that combined an...
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Biomass productivity of Scenedesmus dimorphus (Chlorophyceae) was improved by using an Open Pond–photobioreactor hybrid system
European Journal of Phycology, 2018Co-Authors: Yu Chen, Junfeng WangAbstract:ABSTRACTThe industrialization of microalgae-based biofuel production has been hampered by low biomass productivity of conventional Open Ponds. In this research, a hybrid cultivation system that combined an Open Pond and photobioreactor (PBR), with broth circulating between both, was introduced. The hybrid system was tested under indoor and outdoor conditions using the oleaginous microalgal species Scenedesmus dimorphus. When the PBR(s) in the hybrid system reinforced the light supply to the carbon-replete Open Pond the biomass reached 1.34 g l–1, 116% higher than in the non-hybrid system. Subsequent studies showed that higher circulation speed and low volume ratio of PBR vs. Open Pond would further improve the hybrid effects. When applied outdoors at pilot scale, the biomass productivity of the hybrid system increased 46.3–74.3% compared with the Open Pond and in September was 12.5% higher than that of PBRs. These results indicate that hybrid cultivation might be a cost-effective way to improve the light ...
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The difference in effective light penetration may explain the superiority in photosynthetic efficiency of attached cultivation over the conventional Open Pond for microalgae
Biotechnology for biofuels, 2015Co-Authors: Junfeng Wang, Jinli Liu, Tianzhong LiuAbstract:The ‘attached cultivation’ technique for microalgae production, combining the immobilized biofilm technology with proper light dilution strategies, has shown improved biomass production and photosynthetic efficiency over conventional Open-Pond suspended cultures. However, how light is transferred and distributed inside the biofilm has not been clearly defined yet. In this research, the growth, photosynthetic oxygen evolution, and specific growth rate for microalgal cells in both Open-Pond and attached cultivation were studied to determine the effective light penetration at different phases of the cultivation. As a result, the light conditions inside the culture broth as well as the biofilm were revealed for the first time. Results showed that outdoor, in a conventional 20-cm deep Open Pond, all of the algal cells were fully illuminated in the first 3 days of cultivation. As the biomass concentration increased from day 4 to day 10, the light could only effectively penetrate 45.5% of the Open-Pond depth, and then effective light penetration gradually decreased to 31.1% at day 31, when the biomass density reached a maximum value of 0.45 g L−1 or 90 g m−2. In the attached cultivation system, under nitrogen-replete condition, almost 100% of the immobilized algal cells inside the biofilm were effectively illuminated from day 0 through day 10 when the biomass density increased from 8.8 g m−2 to 107.6 g m−2. Higher light penetration efficiency might be the reason why, using attached cultivation, observed values for photosynthetic efficiency were higher than those recorded in conventional Open-Pond suspended cultures.
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forced light dark circulation operation of Open Pond for microalgae cultivation
Biomass & Bioenergy, 2013Co-Authors: Yu Chen, Junfeng Wang, Wei Zhang, Lin Chen, Lili Gao, Tianzhong LiuAbstract:Open Pond has been widely used for microalgae culture. However its lower biomass productivity damaged its economic viability used as cultivation systems for feedstock production. In this study we introduced a forced L/D circulation operation to conventional Open Pond, in which the culture medium was pumped to circulate between an illuminated shallow Pond and a fully darkened tank. The growth of microalgae was dominated by the photic retention time and dark/light ratio of the forced L/D circulation. The optimal values were determined to be 3.98 for dark/light ratio and 5.80 min for photic retention time by response surface methodology, at which, a biomass productivity of 36.5 g m−2 d−1 for Scenedesmus dimorphus was approached in laboratory. Outdoor cultivation practice with the forced light/dark circulation of Pond was also carried out and averaged 28.5 g m−2 d−1 biomass productivity was achieved, which is double of that by conventional Open Pond cultivations in 250 mm or 50 mm water depth without forced circulation.
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Forced light/dark circulation operation of Open Pond for microalgae cultivation
Biomass and Bioenergy, 2013Co-Authors: Yu Chen, Junfeng Wang, Wei Zhang, Lin Chen, Lili Gao, Tianzhong LiuAbstract:Open Pond has been widely used for microalgae culture. However its lower biomass productivity damaged its economic viability used as cultivation systems for feedstock production. In this study we introduced a forced L/D circulation operation to conventional Open Pond, in which the culture medium was pumped to circulate between an illuminated shallow Pond and a fully darkened tank. The growth of microalgae was dominated by the photic retention time and dark/light ratio of the forced L/D circulation. The optimal values were determined to be 3.98 for dark/light ratio and 5.80 min for photic retention time by response surface methodology, at which, a biomass productivity of 36.5 g m−2 d−1 for Scenedesmus dimorphus was approached in laboratory. Outdoor cultivation practice with the forced light/dark circulation of Pond was also carried out and averaged 28.5 g m−2 d−1 biomass productivity was achieved, which is double of that by conventional Open Pond cultivations in 250 mm or 50 mm water depth without forced circulation.
Tianzhong Liu - One of the best experts on this subject based on the ideXlab platform.
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biomass productivity of scenedesmus dimorphus chlorophyceae was improved by using an Open Pond photobioreactor hybrid system
European Journal of Phycology, 2019Co-Authors: Wen Liu, Yu Chen, Junfeng Wang, Tianzhong LiuAbstract:The industrialization of microalgae-based biofuel production has been hampered by low biomass productivity of conventional Open Ponds. In this research, a hybrid cultivation system that combined an...
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The difference in effective light penetration may explain the superiority in photosynthetic efficiency of attached cultivation over the conventional Open Pond for microalgae
Biotechnology for biofuels, 2015Co-Authors: Junfeng Wang, Jinli Liu, Tianzhong LiuAbstract:The ‘attached cultivation’ technique for microalgae production, combining the immobilized biofilm technology with proper light dilution strategies, has shown improved biomass production and photosynthetic efficiency over conventional Open-Pond suspended cultures. However, how light is transferred and distributed inside the biofilm has not been clearly defined yet. In this research, the growth, photosynthetic oxygen evolution, and specific growth rate for microalgal cells in both Open-Pond and attached cultivation were studied to determine the effective light penetration at different phases of the cultivation. As a result, the light conditions inside the culture broth as well as the biofilm were revealed for the first time. Results showed that outdoor, in a conventional 20-cm deep Open Pond, all of the algal cells were fully illuminated in the first 3 days of cultivation. As the biomass concentration increased from day 4 to day 10, the light could only effectively penetrate 45.5% of the Open-Pond depth, and then effective light penetration gradually decreased to 31.1% at day 31, when the biomass density reached a maximum value of 0.45 g L−1 or 90 g m−2. In the attached cultivation system, under nitrogen-replete condition, almost 100% of the immobilized algal cells inside the biofilm were effectively illuminated from day 0 through day 10 when the biomass density increased from 8.8 g m−2 to 107.6 g m−2. Higher light penetration efficiency might be the reason why, using attached cultivation, observed values for photosynthetic efficiency were higher than those recorded in conventional Open-Pond suspended cultures.
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forced light dark circulation operation of Open Pond for microalgae cultivation
Biomass & Bioenergy, 2013Co-Authors: Yu Chen, Junfeng Wang, Wei Zhang, Lin Chen, Lili Gao, Tianzhong LiuAbstract:Open Pond has been widely used for microalgae culture. However its lower biomass productivity damaged its economic viability used as cultivation systems for feedstock production. In this study we introduced a forced L/D circulation operation to conventional Open Pond, in which the culture medium was pumped to circulate between an illuminated shallow Pond and a fully darkened tank. The growth of microalgae was dominated by the photic retention time and dark/light ratio of the forced L/D circulation. The optimal values were determined to be 3.98 for dark/light ratio and 5.80 min for photic retention time by response surface methodology, at which, a biomass productivity of 36.5 g m−2 d−1 for Scenedesmus dimorphus was approached in laboratory. Outdoor cultivation practice with the forced light/dark circulation of Pond was also carried out and averaged 28.5 g m−2 d−1 biomass productivity was achieved, which is double of that by conventional Open Pond cultivations in 250 mm or 50 mm water depth without forced circulation.
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Forced light/dark circulation operation of Open Pond for microalgae cultivation
Biomass and Bioenergy, 2013Co-Authors: Yu Chen, Junfeng Wang, Wei Zhang, Lin Chen, Lili Gao, Tianzhong LiuAbstract:Open Pond has been widely used for microalgae culture. However its lower biomass productivity damaged its economic viability used as cultivation systems for feedstock production. In this study we introduced a forced L/D circulation operation to conventional Open Pond, in which the culture medium was pumped to circulate between an illuminated shallow Pond and a fully darkened tank. The growth of microalgae was dominated by the photic retention time and dark/light ratio of the forced L/D circulation. The optimal values were determined to be 3.98 for dark/light ratio and 5.80 min for photic retention time by response surface methodology, at which, a biomass productivity of 36.5 g m−2 d−1 for Scenedesmus dimorphus was approached in laboratory. Outdoor cultivation practice with the forced light/dark circulation of Pond was also carried out and averaged 28.5 g m−2 d−1 biomass productivity was achieved, which is double of that by conventional Open Pond cultivations in 250 mm or 50 mm water depth without forced circulation.
Yu Chen - One of the best experts on this subject based on the ideXlab platform.
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biomass productivity of scenedesmus dimorphus chlorophyceae was improved by using an Open Pond photobioreactor hybrid system
European Journal of Phycology, 2019Co-Authors: Wen Liu, Yu Chen, Junfeng Wang, Tianzhong LiuAbstract:The industrialization of microalgae-based biofuel production has been hampered by low biomass productivity of conventional Open Ponds. In this research, a hybrid cultivation system that combined an...
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Biomass productivity of Scenedesmus dimorphus (Chlorophyceae) was improved by using an Open Pond–photobioreactor hybrid system
European Journal of Phycology, 2018Co-Authors: Yu Chen, Junfeng WangAbstract:ABSTRACTThe industrialization of microalgae-based biofuel production has been hampered by low biomass productivity of conventional Open Ponds. In this research, a hybrid cultivation system that combined an Open Pond and photobioreactor (PBR), with broth circulating between both, was introduced. The hybrid system was tested under indoor and outdoor conditions using the oleaginous microalgal species Scenedesmus dimorphus. When the PBR(s) in the hybrid system reinforced the light supply to the carbon-replete Open Pond the biomass reached 1.34 g l–1, 116% higher than in the non-hybrid system. Subsequent studies showed that higher circulation speed and low volume ratio of PBR vs. Open Pond would further improve the hybrid effects. When applied outdoors at pilot scale, the biomass productivity of the hybrid system increased 46.3–74.3% compared with the Open Pond and in September was 12.5% higher than that of PBRs. These results indicate that hybrid cultivation might be a cost-effective way to improve the light ...
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forced light dark circulation operation of Open Pond for microalgae cultivation
Biomass & Bioenergy, 2013Co-Authors: Yu Chen, Junfeng Wang, Wei Zhang, Lin Chen, Lili Gao, Tianzhong LiuAbstract:Open Pond has been widely used for microalgae culture. However its lower biomass productivity damaged its economic viability used as cultivation systems for feedstock production. In this study we introduced a forced L/D circulation operation to conventional Open Pond, in which the culture medium was pumped to circulate between an illuminated shallow Pond and a fully darkened tank. The growth of microalgae was dominated by the photic retention time and dark/light ratio of the forced L/D circulation. The optimal values were determined to be 3.98 for dark/light ratio and 5.80 min for photic retention time by response surface methodology, at which, a biomass productivity of 36.5 g m−2 d−1 for Scenedesmus dimorphus was approached in laboratory. Outdoor cultivation practice with the forced light/dark circulation of Pond was also carried out and averaged 28.5 g m−2 d−1 biomass productivity was achieved, which is double of that by conventional Open Pond cultivations in 250 mm or 50 mm water depth without forced circulation.
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Forced light/dark circulation operation of Open Pond for microalgae cultivation
Biomass and Bioenergy, 2013Co-Authors: Yu Chen, Junfeng Wang, Wei Zhang, Lin Chen, Lili Gao, Tianzhong LiuAbstract:Open Pond has been widely used for microalgae culture. However its lower biomass productivity damaged its economic viability used as cultivation systems for feedstock production. In this study we introduced a forced L/D circulation operation to conventional Open Pond, in which the culture medium was pumped to circulate between an illuminated shallow Pond and a fully darkened tank. The growth of microalgae was dominated by the photic retention time and dark/light ratio of the forced L/D circulation. The optimal values were determined to be 3.98 for dark/light ratio and 5.80 min for photic retention time by response surface methodology, at which, a biomass productivity of 36.5 g m−2 d−1 for Scenedesmus dimorphus was approached in laboratory. Outdoor cultivation practice with the forced light/dark circulation of Pond was also carried out and averaged 28.5 g m−2 d−1 biomass productivity was achieved, which is double of that by conventional Open Pond cultivations in 250 mm or 50 mm water depth without forced circulation.
Eiichiro Fukusaki - One of the best experts on this subject based on the ideXlab platform.
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metabolite profiling of whiteleg shrimp litOpenaeus vannamei from super intensive culture in closed aquaculture systems a recirculating aquaculture system and a hybrid zero water discharge recirculating aquaculture system
Metabolomics, 2020Co-Authors: Gede Suantika, Magdalena Lenny Situmorang, Felicia Irene Saputra, Safira Latifa Erlangga Putri, Pingkan Aditiawati, Sastia Prama Putri, Eiichiro FukusakiAbstract:INTRODUCTION: The production of the whiteleg shrimp LitOpenaeus vannamei now accounts for approximately 75% of the total shrimp production in Indonesia. The techniques used to produce whiteleg shrimp in Indonesia are still dominated by conventional rearing strategies using Open-Pond systems, which often contribute to unpredictable culture performance and weak sustainability. Alternative production strategies of closed aquaculture systems, including the recirculating aquaculture system (RAS) and hybrid zero water discharge-recirculating aquaculture system (hybrid system), have been developed and implemented for higher productivity, stability and sustainability of whiteleg shrimp grow-out production in Indonesia. Despite the positive aspects of the application of closed aquaculture systems in shrimp aquaculture, the differences in the characteristics of shrimp grown in closed RAS and hybrid systems compared to Open-Pond systems remain unclear. OBJECTIVE: This study aims to investigate the differences in the metabolite profiles of shrimp grown in intensive closed aquaculture systems, including an RAS and hybrid system, compared to those of shrimp grown in a semi-intensive, Open, earthen Pond system by means of non-targeted GC-MS metabolite profiling. METHODS: Shrimp cultured in the closed systems (RAS and hybrid system) and an Open system (Pond) were harvested and subjected to GC-MS non-targeted metabolomics analysis. A total of 112 metabolites were annotated from shrimp samples and subjected to principal component analysis (PCA). RESULTS: The metabolites annotated from GC-MS mainly included organic compounds, proteinogenic and non-proteinogenic amino acids, sugars, nucleosides and fatty acids. The results of principal component analysis showed several metabolites with high variable importance in projection (VIP) scores, including shikimic acid, β-alanine, uric acid, hypoxanthine, inosine, homocysteine, methionine, phenylalanine, tryptophan and lysine, as the main metabolites differentiating the shrimp grown in the three production systems. CONCLUSION: Our findings showed that shrimp cultured in different aquaculture systems exhibited distinct metabolite profiles, and the metabolites showing high VIP scores, including shikimic acid, β-alanine, uric acid, hypoxanthine, inosine, homocysteine, methionine, phenylalanine, tryptophan and lysine, may serve as candidate markers to indicate the differences in shrimp from different production systems.
Hee-sik Kim - One of the best experts on this subject based on the ideXlab platform.
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novel biological method for controlling bacterial contaminants using the ciliate colpoda sp hsp 001 in Open Pond algal cultivation
Biomass & Bioenergy, 2019Co-Authors: Hee-sik Kim, Kichul Cho, Sangmoo Lee, Daehyun Cho, Jina Heo, Yong Jae LeeAbstract:Abstract Outdoor algal cultivation is frequently limited by bacterial contaminants. The aim of this study was to develop a biological control technique for removing bacterial contaminants using a small-size ciliate. During the cultivation of Chlorell a sp. HS2 in a designed raceway Pond, the bacterial population was significantly reduced by the ciliate strain. 18S rDNA analysis identified the ciliate as Colpoda sp. HSP-001. To verify selective bacterial grazing by the ciliate, an isolated phycospheric bacterium Microbacterium sp. was co-cultivated with Chlorella sp. HS2 while varying the ciliate cell concentrations. Colpoda sp. efficiently eliminated bacterial cells, whereas no algal grazing was observed by fluorescence microscopy. Based on the results, the utilization of Colpoda sp. HSP-001 in an outdoor algal cultivation system is a cost-effective strategy for controlling bacterial contamination without degrading the algal biomass.
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Municipal Wastewater Treatment and Microbial Diversity Analysis of Microalgal Mini Raceway Open Pond
The Korean Journal of Microbiology, 2012Co-Authors: Zion Kang, Byung-hyuk Kim, Sang Yoon Shin, Hee-sik KimAbstract:Microalgal biotechnology has gained prominence because of the ability of microalgae to produce value–added products including biodiesel through photosynthesis. However, carbon and nutrient source is often a limiting factor for microalgal growth leading to higher input costs for sufficient biomass production. Use of municipal wastewater as a low cost alternative to grow microalgae as well as to treat the same has been demonstrated in this study using mini raceway Open Ponds. Municipal wastewater was collected after primary treatment and microalgae indigenous in the wastewater were encouraged to grow in Open raceways under optimum conditions. The mean removal efficiencies of TN, TP, COD-
