The Experts below are selected from a list of 4050 Experts worldwide ranked by ideXlab platform

Gadadhar Parida - One of the best experts on this subject based on the ideXlab platform.

  • keratinized strap cells a rare cytological atypia resembles anitschkow cells in human Oral Neoplasm
    International Journal of Clinical Oncology, 2016
    Co-Authors: Abhimanyu Mohanta, Prafulla K Mohanty, Gadadhar Parida
    Abstract:

    Cytopathology of human Oral squamous cell carcinoma reveals cytological pleomorphism. In this investigation, cytometry was used in an attempt to analyse the importance of the rare occurrence of keratinized strap cells in the buccal mucosa of human Oral Neoplasms. A case–control study was undertaken in which exfoliated cytosmears were collected, by scraping, from 136 clinically diagnosed Oral cancer patients. Wet fixed cytosmears were stained by use of Papanicolaou’s staining  procedure and counter-stained with Giemsa’s solution. One thousand cells were screened, and keratinized strap (Anitschkow) cells (KSC-A) and other cytological atypias were counted. Cytomorphometry was performed by use of a computer-assisted microscope camera. The findings were analysed statistically and interpreted in respect of age group, Oral site, and sex. Transformation of an angulated and polygonal normal Oral squamous cell into a keratinized narrow strip or ribbon-like flat projection results in what is called a keratinized strap cell (KSC). Nuclear pleomorphism in keratinized strap cells was observed to be narrow spindle, rod, and bar-shaped and to resemble Anitschkow cells. From the nature of the staining, the structural peculiarity, and the resemblance to Anitschkow cells, KSC, a rarely observed Oral cytological atypia, were renamed as keratinized strap (Anitschkow) (KSC-A) cells. Cytometrically, the nuclear-to-cytoplasmic (N/C) ratio for KSC-A was calculated to be 1:11.2 for males and 1:11.3 for females; these are more than those for the normal counterparts. Rare occurrence, cellular keratinization, nuclear pleomorphism, hyperchromasia, and increased N/C ratios for KSC-A in Oral carcinoma, for both sexes, indicate a state of malignancy; this finding is, thus, of practical value for early detection and diagnosis of Oral squamous cell carcinoma patients.

  • keratinized tadpole cells in human Oral Neoplasm a cytodiagnostic approach
    IOSR Journal of Dental and Medical Sciences, 2014
    Co-Authors: Abhimanyu Mohanta, Prafulla K Mohanty, Gadadhar Parida
    Abstract:

    Background: Use of exfoliative cytology and cyto-analysis is a useful early diagnostic method for epithelial atypia and for human Oral Neoplasm. A rare occurrence of cytological atypia appeared to be anuran tadpole larva, named as keratinized tadpole cell (KTC) is analyzed cytomorphometrically with a cytodiagnostic approach. Materials and Methods: In a case- control study, exfoliated scraped cytosmears were collected from the affected sites of the clinically diagnosed 136 Oral cancer patients over the pre-cleaned microslides and immediately fixed in acetoalcohol. Cytosmears were stained with routine Papanicolaou’s stain and counterstained with the Giemsa’s solution. One thousand cells were observed and the KTCs were scored from each stained sample following standard criteria. Findings were statistically analyzed, interpreted and correlated with the Oral sites, sex and nature of addiction. Results: Keratinized tadpole cells (KTCs) exhibit both cytological and nuclear pleomorphism. Almost all KTCs are moderately differentiated with a well defined head and tail in each. Usually, nucleus is confined to the head part of the cell and tail contains cytoplasm only. Keratinized cytoplasm, nuclear pleomorphism, presence of nuclear haloes, micronucleation, multinucleation and increased nuclear-cytoplasmic ratios (N/C) in keratinized tadpole cells are the clear indication of malignancy so far as cytopathology is concerned. Conclusion: Appearance of KTCs in the epithelia of Oral Neoplasm has a practical utility in early detection and diagnosis of the Oral cancer patients.

Abhimanyu Mohanta - One of the best experts on this subject based on the ideXlab platform.

  • Nuclear pleomorphism-based cytopathological grading in human Oral Neoplasm
    Limited liability company «Science and Innovations» (Saratov), 2017
    Co-Authors: Abhimanyu Mohanta, Prafulla K Mohanty
    Abstract:

    Objective ― The objective of the present study is record the nuclear pleomorphism in various stages of Oral carcinogenesis and to analyse their utility in cytopathological grading for early detection of human Oral cancer Material and Methods ― In this hospital based case-control study, Oral site, age-group and sex-matched 272 subjects (136 cases and 136 normal healthy individuals) were included. Scraped exfoliated cytosmears were collected from the affected Oral site of the subjects and smearing was done in the pre-cleaned-coded glass-slides. Two such slides were prepared from each subject. The cytosmears were immediately fixed in aceto-alcohol (1 part of glacial acetic acid: 3 part of absolute ethyl alcohol) fixative. One set of the slide was stained with Papanicolaou’s stain and the other set was counter-stained with Giemsa’s Solution for cytopathological analysis. Test of proportion (z-test) was followed and the critical ratio (z-value) was calculated for the test of significance. Results ― Nuclear pleomorphism in the form of round, oval, spindle, elongated fiber as well as irregular shapes were mostly observed in Oral squamous cells during different stages of carcinogenesis. Appearance of such nuclear pleomorphism in human Oral Neoplasm may be considered as a sign of cellular alternation in general and index of Oral carcinogenesis in particular. In the present study, the frank malignant cases mimic to be either premalignant lesions or benign/carcinoma in situ were detected on the basis of nuclear pleomorphism-based cytopathological grading and so an increasing trend was observed from precancerous lesions to malignant cases due to shifting of numbers. Diagnostic tests also indicated that the Sensitivity was calculated to be 83.5%, Specificity was 100%, positive predictive value (PPV) was 100%, negative predictive value (NPV) was 30% and the accuracy was found to be 84.6%. Therefore, the nuclear pleomorphism-based cytopathological grading system makes itself an ideal screening test for early detection of human Oral cancer. Conclusion ― Pattern of nuclear pleomorphism corresponding to various cytological atypias is a common feature observed during different stages of Oral carcinogenesis and thus, it has a practical implication in grading and early detection of Oral cancer

  • keratinized strap cells a rare cytological atypia resembles anitschkow cells in human Oral Neoplasm
    International Journal of Clinical Oncology, 2016
    Co-Authors: Abhimanyu Mohanta, Prafulla K Mohanty, Gadadhar Parida
    Abstract:

    Cytopathology of human Oral squamous cell carcinoma reveals cytological pleomorphism. In this investigation, cytometry was used in an attempt to analyse the importance of the rare occurrence of keratinized strap cells in the buccal mucosa of human Oral Neoplasms. A case–control study was undertaken in which exfoliated cytosmears were collected, by scraping, from 136 clinically diagnosed Oral cancer patients. Wet fixed cytosmears were stained by use of Papanicolaou’s staining  procedure and counter-stained with Giemsa’s solution. One thousand cells were screened, and keratinized strap (Anitschkow) cells (KSC-A) and other cytological atypias were counted. Cytomorphometry was performed by use of a computer-assisted microscope camera. The findings were analysed statistically and interpreted in respect of age group, Oral site, and sex. Transformation of an angulated and polygonal normal Oral squamous cell into a keratinized narrow strip or ribbon-like flat projection results in what is called a keratinized strap cell (KSC). Nuclear pleomorphism in keratinized strap cells was observed to be narrow spindle, rod, and bar-shaped and to resemble Anitschkow cells. From the nature of the staining, the structural peculiarity, and the resemblance to Anitschkow cells, KSC, a rarely observed Oral cytological atypia, were renamed as keratinized strap (Anitschkow) (KSC-A) cells. Cytometrically, the nuclear-to-cytoplasmic (N/C) ratio for KSC-A was calculated to be 1:11.2 for males and 1:11.3 for females; these are more than those for the normal counterparts. Rare occurrence, cellular keratinization, nuclear pleomorphism, hyperchromasia, and increased N/C ratios for KSC-A in Oral carcinoma, for both sexes, indicate a state of malignancy; this finding is, thus, of practical value for early detection and diagnosis of Oral squamous cell carcinoma patients.

  • cytomorphometric analysis of non keratinized malignant squamous cells in exfoliated cytosmears of human Oral Neoplasm
    Journal of carcinogenesis & mutagenesis, 2016
    Co-Authors: Abhimanyu Mohanta, Prafulla K Mohanty
    Abstract:

    Objectives: During the present investigation, a unique type of pleomorphic cytological atypia- named as nonkeratinized malignant squamous cell (NMSC) was frequently observed in the exfoliated cytosmears of the Oral cancer patients. Details of these peculiar atypical, pleomorphic cells have not been reported earlier. Therefore, an attempt has been undertaken to find out the practical utility of NMSCs during difficult diagnoses and in early detection of Oral squamous cell carcinoma (OSCC) cases through cytomorphometric analysis. Materials and Methods: In a hospital-based case-control study exfoliated scrape smears were collected from the clinically diagnosed 136 patients suffering from precancerous lesions and cancerous cases and a parallel set of 136 samples were also collected from the non-addicted and non-cancerous healthy individuals considered as control group. Wet fixed smears were stained by adopting Papanicolaou’s staining protocol and counter-stained with Giemsa’s solution. Out of one thousand screened cells, frequently observed NMSCs were scored. Cytomorphometry was done by using computer-assisted Cat Cam 1.30 (1.3 Mega Pixel) Microscope Camera® fitted with hund®-H500 research binocular microscope. The findings were statistically analyzed and interpreted by using software package PAleontological Statistics (PAST)® Version 2.17 with respect to age groups, degree of pathogenicity, Oral sites and sexes. Results: Cytomorphometrically, the cellular parameters (mean length, breadth and area) of the NMSC were found to be in decreased state and nuclear parameters are in increased state. The quantitative parameters of the NMSCs were significantly (p ≤ 0.01) decreased with increasing age groups. Cytomorphometrically, the nuclearcytoplasmic (N/C) ratio of the NMSC was found to be 1:1 in both sexes. Conclusion: Extreme reduction of cellular diameter and increased nuclear diameter in each NMSC, lead to cellular non-keratinization, hyperchromasia and increased N/C ratios in both sexes indicate the state of malignancy. Thus, the present finding has a practical utility in early detection and diagnosis of the OSCC patients.

  • keratinized tadpole cells in human Oral Neoplasm a cytodiagnostic approach
    IOSR Journal of Dental and Medical Sciences, 2014
    Co-Authors: Abhimanyu Mohanta, Prafulla K Mohanty, Gadadhar Parida
    Abstract:

    Background: Use of exfoliative cytology and cyto-analysis is a useful early diagnostic method for epithelial atypia and for human Oral Neoplasm. A rare occurrence of cytological atypia appeared to be anuran tadpole larva, named as keratinized tadpole cell (KTC) is analyzed cytomorphometrically with a cytodiagnostic approach. Materials and Methods: In a case- control study, exfoliated scraped cytosmears were collected from the affected sites of the clinically diagnosed 136 Oral cancer patients over the pre-cleaned microslides and immediately fixed in acetoalcohol. Cytosmears were stained with routine Papanicolaou’s stain and counterstained with the Giemsa’s solution. One thousand cells were observed and the KTCs were scored from each stained sample following standard criteria. Findings were statistically analyzed, interpreted and correlated with the Oral sites, sex and nature of addiction. Results: Keratinized tadpole cells (KTCs) exhibit both cytological and nuclear pleomorphism. Almost all KTCs are moderately differentiated with a well defined head and tail in each. Usually, nucleus is confined to the head part of the cell and tail contains cytoplasm only. Keratinized cytoplasm, nuclear pleomorphism, presence of nuclear haloes, micronucleation, multinucleation and increased nuclear-cytoplasmic ratios (N/C) in keratinized tadpole cells are the clear indication of malignancy so far as cytopathology is concerned. Conclusion: Appearance of KTCs in the epithelia of Oral Neoplasm has a practical utility in early detection and diagnosis of the Oral cancer patients.

Prafulla K Mohanty - One of the best experts on this subject based on the ideXlab platform.

  • Nuclear pleomorphism-based cytopathological grading in human Oral Neoplasm
    Limited liability company «Science and Innovations» (Saratov), 2017
    Co-Authors: Abhimanyu Mohanta, Prafulla K Mohanty
    Abstract:

    Objective ― The objective of the present study is record the nuclear pleomorphism in various stages of Oral carcinogenesis and to analyse their utility in cytopathological grading for early detection of human Oral cancer Material and Methods ― In this hospital based case-control study, Oral site, age-group and sex-matched 272 subjects (136 cases and 136 normal healthy individuals) were included. Scraped exfoliated cytosmears were collected from the affected Oral site of the subjects and smearing was done in the pre-cleaned-coded glass-slides. Two such slides were prepared from each subject. The cytosmears were immediately fixed in aceto-alcohol (1 part of glacial acetic acid: 3 part of absolute ethyl alcohol) fixative. One set of the slide was stained with Papanicolaou’s stain and the other set was counter-stained with Giemsa’s Solution for cytopathological analysis. Test of proportion (z-test) was followed and the critical ratio (z-value) was calculated for the test of significance. Results ― Nuclear pleomorphism in the form of round, oval, spindle, elongated fiber as well as irregular shapes were mostly observed in Oral squamous cells during different stages of carcinogenesis. Appearance of such nuclear pleomorphism in human Oral Neoplasm may be considered as a sign of cellular alternation in general and index of Oral carcinogenesis in particular. In the present study, the frank malignant cases mimic to be either premalignant lesions or benign/carcinoma in situ were detected on the basis of nuclear pleomorphism-based cytopathological grading and so an increasing trend was observed from precancerous lesions to malignant cases due to shifting of numbers. Diagnostic tests also indicated that the Sensitivity was calculated to be 83.5%, Specificity was 100%, positive predictive value (PPV) was 100%, negative predictive value (NPV) was 30% and the accuracy was found to be 84.6%. Therefore, the nuclear pleomorphism-based cytopathological grading system makes itself an ideal screening test for early detection of human Oral cancer. Conclusion ― Pattern of nuclear pleomorphism corresponding to various cytological atypias is a common feature observed during different stages of Oral carcinogenesis and thus, it has a practical implication in grading and early detection of Oral cancer

  • keratinized strap cells a rare cytological atypia resembles anitschkow cells in human Oral Neoplasm
    International Journal of Clinical Oncology, 2016
    Co-Authors: Abhimanyu Mohanta, Prafulla K Mohanty, Gadadhar Parida
    Abstract:

    Cytopathology of human Oral squamous cell carcinoma reveals cytological pleomorphism. In this investigation, cytometry was used in an attempt to analyse the importance of the rare occurrence of keratinized strap cells in the buccal mucosa of human Oral Neoplasms. A case–control study was undertaken in which exfoliated cytosmears were collected, by scraping, from 136 clinically diagnosed Oral cancer patients. Wet fixed cytosmears were stained by use of Papanicolaou’s staining  procedure and counter-stained with Giemsa’s solution. One thousand cells were screened, and keratinized strap (Anitschkow) cells (KSC-A) and other cytological atypias were counted. Cytomorphometry was performed by use of a computer-assisted microscope camera. The findings were analysed statistically and interpreted in respect of age group, Oral site, and sex. Transformation of an angulated and polygonal normal Oral squamous cell into a keratinized narrow strip or ribbon-like flat projection results in what is called a keratinized strap cell (KSC). Nuclear pleomorphism in keratinized strap cells was observed to be narrow spindle, rod, and bar-shaped and to resemble Anitschkow cells. From the nature of the staining, the structural peculiarity, and the resemblance to Anitschkow cells, KSC, a rarely observed Oral cytological atypia, were renamed as keratinized strap (Anitschkow) (KSC-A) cells. Cytometrically, the nuclear-to-cytoplasmic (N/C) ratio for KSC-A was calculated to be 1:11.2 for males and 1:11.3 for females; these are more than those for the normal counterparts. Rare occurrence, cellular keratinization, nuclear pleomorphism, hyperchromasia, and increased N/C ratios for KSC-A in Oral carcinoma, for both sexes, indicate a state of malignancy; this finding is, thus, of practical value for early detection and diagnosis of Oral squamous cell carcinoma patients.

  • cytomorphometric analysis of non keratinized malignant squamous cells in exfoliated cytosmears of human Oral Neoplasm
    Journal of carcinogenesis & mutagenesis, 2016
    Co-Authors: Abhimanyu Mohanta, Prafulla K Mohanty
    Abstract:

    Objectives: During the present investigation, a unique type of pleomorphic cytological atypia- named as nonkeratinized malignant squamous cell (NMSC) was frequently observed in the exfoliated cytosmears of the Oral cancer patients. Details of these peculiar atypical, pleomorphic cells have not been reported earlier. Therefore, an attempt has been undertaken to find out the practical utility of NMSCs during difficult diagnoses and in early detection of Oral squamous cell carcinoma (OSCC) cases through cytomorphometric analysis. Materials and Methods: In a hospital-based case-control study exfoliated scrape smears were collected from the clinically diagnosed 136 patients suffering from precancerous lesions and cancerous cases and a parallel set of 136 samples were also collected from the non-addicted and non-cancerous healthy individuals considered as control group. Wet fixed smears were stained by adopting Papanicolaou’s staining protocol and counter-stained with Giemsa’s solution. Out of one thousand screened cells, frequently observed NMSCs were scored. Cytomorphometry was done by using computer-assisted Cat Cam 1.30 (1.3 Mega Pixel) Microscope Camera® fitted with hund®-H500 research binocular microscope. The findings were statistically analyzed and interpreted by using software package PAleontological Statistics (PAST)® Version 2.17 with respect to age groups, degree of pathogenicity, Oral sites and sexes. Results: Cytomorphometrically, the cellular parameters (mean length, breadth and area) of the NMSC were found to be in decreased state and nuclear parameters are in increased state. The quantitative parameters of the NMSCs were significantly (p ≤ 0.01) decreased with increasing age groups. Cytomorphometrically, the nuclearcytoplasmic (N/C) ratio of the NMSC was found to be 1:1 in both sexes. Conclusion: Extreme reduction of cellular diameter and increased nuclear diameter in each NMSC, lead to cellular non-keratinization, hyperchromasia and increased N/C ratios in both sexes indicate the state of malignancy. Thus, the present finding has a practical utility in early detection and diagnosis of the OSCC patients.

  • keratinized tadpole cells in human Oral Neoplasm a cytodiagnostic approach
    IOSR Journal of Dental and Medical Sciences, 2014
    Co-Authors: Abhimanyu Mohanta, Prafulla K Mohanty, Gadadhar Parida
    Abstract:

    Background: Use of exfoliative cytology and cyto-analysis is a useful early diagnostic method for epithelial atypia and for human Oral Neoplasm. A rare occurrence of cytological atypia appeared to be anuran tadpole larva, named as keratinized tadpole cell (KTC) is analyzed cytomorphometrically with a cytodiagnostic approach. Materials and Methods: In a case- control study, exfoliated scraped cytosmears were collected from the affected sites of the clinically diagnosed 136 Oral cancer patients over the pre-cleaned microslides and immediately fixed in acetoalcohol. Cytosmears were stained with routine Papanicolaou’s stain and counterstained with the Giemsa’s solution. One thousand cells were observed and the KTCs were scored from each stained sample following standard criteria. Findings were statistically analyzed, interpreted and correlated with the Oral sites, sex and nature of addiction. Results: Keratinized tadpole cells (KTCs) exhibit both cytological and nuclear pleomorphism. Almost all KTCs are moderately differentiated with a well defined head and tail in each. Usually, nucleus is confined to the head part of the cell and tail contains cytoplasm only. Keratinized cytoplasm, nuclear pleomorphism, presence of nuclear haloes, micronucleation, multinucleation and increased nuclear-cytoplasmic ratios (N/C) in keratinized tadpole cells are the clear indication of malignancy so far as cytopathology is concerned. Conclusion: Appearance of KTCs in the epithelia of Oral Neoplasm has a practical utility in early detection and diagnosis of the Oral cancer patients.

Lelia Batista De Souza - One of the best experts on this subject based on the ideXlab platform.

  • kras mutations drive adenomatoid odontogenic tumor and are independent of clinicopathological features
    Modern Pathology, 2019
    Co-Authors: Bruna Pizziolo Coura, Vanessa Fatima Bernardes, Silvia Ferreira De Sousa, Josiane Alves Franca, Nubia Braga Pereira, Helder Antonio Rebelo Pontes, Aline Carvalho Batista, Danyel Elias Da Cruz Perez, Ricardo Luiz Cavalcanti De Albuquerque, Lelia Batista De Souza
    Abstract:

    Adenomatoid odontogenic tumor is a benign encapsulated epithelial odontogenic tumor that shows an indolent clinical behavior. We have reported in a few adenomatoid odontogenic tumors mutations in KRAS, which is a proto-oncogene frequently mutated in cancer such as lung, pancreas, and colorectal adenocarcinomas. We aimed to assess KRAS mutations in the hotspot codons 12, 13, and 61 in a large cohort of adenomatoid odontogenic tumors and to test the association of these mutations with clinical (age, site, tumor size, follicular/extrafollicular subtypes) and histopathological parameters. Thirty eight central cases were studied. KRAS codon 12 mutations were assessed by TaqMan allele-specific qPCR (p.G12V/R) and/or Sanger sequencing, and codon 13 and 61 mutations were screened by Sanger. Histological tumor capsule thickness was evaluated by morphometric analysis. Additionally, the phosphorylated form of the MAPK downstream effector ERK1/2 was investigated. Statistical analysis was carried out to test the association of KRAS mutations with clinicopathological parameters. KRAS c.35 G >T mutation, leading to p.G12V, was detected in 15 cases. A novel mutation in adenomatoid odontogenic tumor, c.34 G >C, leading to p.G12R, was detected in 12 cases and the other 11 were wild-type. Codon 12 mutations were not associated with the clinicopathological parameters tested. RAS mutations are known to activate the MAPK pathway, and we show that adenomatoid odontogenic tumors express phosphorylated ERK1/2. In conclusion, a high proportion of adenomatoid odontogenic tumors (27/38, 71%) have KRAS codon 12 mutations, which occur independently of the clinicopathological features evaluated. Collectively, these findings indicate that KRAS mutations and MAPK pathway activation are the common features of this tumor and some cancer types. Although it is unclear why different codon 12 alleles occur in different disease contexts and the complex interactions between tumor genotype and phenotype need clarification, on the basis of our results the presence of KRAS p.G12V/R favors the adenomatoid odontogenic tumor diagnosis in challenging Oral Neoplasm cases.

Qianming Chen - One of the best experts on this subject based on the ideXlab platform.

  • association of increased circulating catecholamine and glucocorticoid levels with risk of psychological problems in Oral Neoplasm patients
    PLOS ONE, 2014
    Co-Authors: Huixu Xie, Xiaoli Zou, Cairong Zhu, Ning Gao, Qianming Chen
    Abstract:

    Background Noradrenergic pathways and glucocorticoid-mediated signal pathways have been implicated in the growth and progression of Oral cancer. Patients with Oral Neoplasms can have high psychological distress levels, but the effects of stress-related hormones on Oral Neoplasm growth are unknown. Methods We have investigated the relationships between pre-surgical measurements of psychological problems with Symptom Checklist-90-revised Inventory (SCL90-R), tumor histology, circulating blood catecholamine and glucocorticoid levels among 75 Oral Neoplasm patients, including 40 Oral cancer patients and 35 benign Oral tumor patients. Results The results showed that most dimension scores of SCL90-R did not show a significant difference between the two groups except depression (p = 0.0201) and obsessive-compulsion (p = 0.0093), with the scores for these symptoms being higher among Oral cancer group versus the benign Oral tumor group. The differences of total score, average score and other monomial factor scores were not statistically significant. The mean concentrations of catecholamine and glucocorticoid in peripheral blood of the Oral cancer group were higher than those in benign Oral tumor group (p<0.01). We also examined whether associations observed between biobehaviOral measures and circulating blood catecholamine and glucocorticoid levels extended to other compartments in the Oral cancer group. Conclusions These findings suggest that stress hormones may affect Oral cancer behavior by influencing the tumor micro-environment though the circulating blood.