The Experts below are selected from a list of 4086 Experts worldwide ranked by ideXlab platform
Xinhai Ye - One of the best experts on this subject based on the ideXlab platform.
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isolation cultivation and identification of skeletal Muscle stem cells derived from human Orbicularis Oculi Muscle
Chinese journal of plastic surgery, 2019Co-Authors: Caihe Liao, Xinhai YeAbstract:Objective To establish the isolation and culture methods of skeletal Muscle stem cells, derived from human Orbicularis Oculi Muscle (OOMSCs), and to identify their multi-directional differentiation potential in vitro. Methods The cellswere isolated from pretarsal Orbicularis Oculi Muscle (OOM), obtained in routine blepharoplasty surgery.The tissue was digested using collagenase type I combined with re-plating methods. Specific cell surface antigen markers were detected using flow cytometry analysis. OOMSCs were cultured under different inductive conditions, to identify their pluripotent differentiation ability. Results OOMSCs exhibited similar fibroblast-like morphology as mesenchymal stem cells with high expression of skeletal Muscle-derived stem cell surface markers. OOMSCs were able to differentiate into adipocytes, osteoblasts and chondrocytes in vitro, in the presence of lineage-specific inductive media. Moreover, after myogenic induction, the differentiated cells were fused into multinucleated myotube-like structure, and positive for myogenic-related marks, Pax3, Pax7, Myf5 and MyoD. Conclusions Muscle-derived stem cells can be isolated from human OOM with myogenic differentiation properties, showing further applications potential intissue regeneration and medical therapies of Muscle diseases. Key words: Orbicularis Oculi Muscle; Skeletal Muscle-derived stem cells; Isolation and culture; Myogenic differentiation; Neuromuscular diseases
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identification and characterization of skeletal Muscle stem cells from human Orbicularis Oculi Muscle
Tissue Engineering Part C-methods, 2018Co-Authors: Caihe Liao, Xi Chen, Yipin Xu, Xinhai YeAbstract:Skeletal Muscle stem cell (SMSC) transplantation has shown great therapeutical potential in repairing Muscle loss and dysfunction, but the Muscle acquisition is usually a traumatic procedure causing pain and morbidity to the donor. In this study, we investigated the feasibility of isolating SMSCs from human Orbicularis Oculi Muscle (OOM), which is routinely removed and discarded during ophthalmic cosmetic surgeries. OOM fragments were harvested from 18 female healthy donors undergoing upper eyelid plasties. Plastic-adherent cells were isolated from the Muscles using a two-step plating method combined with collagenase digestion. A total of 15 cell cultures were successfully established from the Muscle samples. These adherent cells were positive for the specific markers of SMSCs and could be directed toward the osteogenic, adipogenic, chondrogenic, and myogenic phenotypes in the presence of lineage-specific inductive media. Moreover, after cultured in the myogenic inductive medium for 3 weeks, the Muscle ce...
Caihe Liao - One of the best experts on this subject based on the ideXlab platform.
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isolation cultivation and identification of skeletal Muscle stem cells derived from human Orbicularis Oculi Muscle
Chinese journal of plastic surgery, 2019Co-Authors: Caihe Liao, Xinhai YeAbstract:Objective To establish the isolation and culture methods of skeletal Muscle stem cells, derived from human Orbicularis Oculi Muscle (OOMSCs), and to identify their multi-directional differentiation potential in vitro. Methods The cellswere isolated from pretarsal Orbicularis Oculi Muscle (OOM), obtained in routine blepharoplasty surgery.The tissue was digested using collagenase type I combined with re-plating methods. Specific cell surface antigen markers were detected using flow cytometry analysis. OOMSCs were cultured under different inductive conditions, to identify their pluripotent differentiation ability. Results OOMSCs exhibited similar fibroblast-like morphology as mesenchymal stem cells with high expression of skeletal Muscle-derived stem cell surface markers. OOMSCs were able to differentiate into adipocytes, osteoblasts and chondrocytes in vitro, in the presence of lineage-specific inductive media. Moreover, after myogenic induction, the differentiated cells were fused into multinucleated myotube-like structure, and positive for myogenic-related marks, Pax3, Pax7, Myf5 and MyoD. Conclusions Muscle-derived stem cells can be isolated from human OOM with myogenic differentiation properties, showing further applications potential intissue regeneration and medical therapies of Muscle diseases. Key words: Orbicularis Oculi Muscle; Skeletal Muscle-derived stem cells; Isolation and culture; Myogenic differentiation; Neuromuscular diseases
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identification and characterization of skeletal Muscle stem cells from human Orbicularis Oculi Muscle
Tissue Engineering Part C-methods, 2018Co-Authors: Caihe Liao, Xi Chen, Yipin Xu, Xinhai YeAbstract:Skeletal Muscle stem cell (SMSC) transplantation has shown great therapeutical potential in repairing Muscle loss and dysfunction, but the Muscle acquisition is usually a traumatic procedure causing pain and morbidity to the donor. In this study, we investigated the feasibility of isolating SMSCs from human Orbicularis Oculi Muscle (OOM), which is routinely removed and discarded during ophthalmic cosmetic surgeries. OOM fragments were harvested from 18 female healthy donors undergoing upper eyelid plasties. Plastic-adherent cells were isolated from the Muscles using a two-step plating method combined with collagenase digestion. A total of 15 cell cultures were successfully established from the Muscle samples. These adherent cells were positive for the specific markers of SMSCs and could be directed toward the osteogenic, adipogenic, chondrogenic, and myogenic phenotypes in the presence of lineage-specific inductive media. Moreover, after cultured in the myogenic inductive medium for 3 weeks, the Muscle ce...
Holger Markus - One of the best experts on this subject based on the ideXlab platform.
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Cortical representation of the Orbicularis Oculi Muscle as assessed by transcranial magnetic stimulation (TMS).
The Laryngoscope, 2020Co-Authors: Ralph M W Roedel, R Laskawi, Holger MarkusAbstract:To analyze characteristic features and details on motor-evoked potentials (MEPs) of the Orbicularis Oculi Muscle resulting from cortical transcranial magnetic stimulation (TMS) in normal subjects as a basis for further investigations on motorcortical representation in patients presenting with facial nerve diseases. MEPs of the Orbicularis Oculi Muscle resulting from focal cortical TMS with a figure-8-shaped coil were investigated in 17 healthy subjects with special regard to amplitude and onset latency as a function of the coil position on the head surface along the interaural line and in the anterior-posterior direction. The results were then compared with our data on lower-lip mimetic Muscles and on the frontalis Muscle obtained in previous studies. Bilateral reproducible responses could be observed at coil positions varying from 1 to 13 cm lateral to the vertex. During moderate Muscle activation, maximum responses (mean amplitude 0.75 +/- 0.44 mV contralateral, 0.74 +/- 0.36 mV ipsilateral) were obtained at a mean stimulus position of 8.6 +/- 1.6 cm lateral and 2.0 +/- 2.2 cm anterior to the vertex for contralateral responses, and of 8.6 +/- 2.0 cm lateral and 2.8 +/- 2.4 cm anterior to the vertex for ipsilateral responses, respectively. Voluntary Muscle activation by forced eye-closure was associated with a further increase in mean amplitudes. At rest, bilateral responses could be elicited in 15 subjects (88.2%). During moderate Muscle activation, the shortest mean onset latencies were obtained at the optimum stimulus position on the interaural line, both for contralateral (10.2 +/- 1.3 ms) and ipsilateral (10.6 +/- 1.5 ms) MEPs. Comparing our data on the Orbicularis Oculi Muscle with those obtained on lower-lip Muscles and on the frontalis Muscle, there was a considerable overlap of coil positions from which reproducible MEPs could be elicited in all three groups of mimetic Muscles, but with the Orbicularis Oculi area being placed between forehead and lower-lip motorcortical areas. A statistically significant separation of the cortical representation areas of forehead, Orbicularis oris, and lower-lip mimetic Muscles is possible by focal cortical TMS reflecting a kind of somatotopic organization of the face-associated motorcortex. Compared with the results on lower-lip and forehead Muscles, Orbicularis Oculi Muscle responses show characteristics of both.
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cortical representation of the Orbicularis Oculi Muscle as assessed by transcranial magnetic stimulation tms
Laryngoscope, 2001Co-Authors: Ralph M W Roedel, R Laskawi, Holger MarkusAbstract:Objectives To analyze characteristic features and details on motor-evoked potentials (MEPs) of the Orbicularis Oculi Muscle resulting from cortical transcranial magnetic stimulation (TMS) in normal subjects as a basis for further investigations on motorcortical representation in patients presenting with facial nerve diseases. Study Design MEPs of the Orbicularis Oculi Muscle resulting from focal cortical TMS with a figure-8-shaped coil were investigated in 17 healthy subjects with special regard to amplitude and onset latency as a function of the coil position on the head surface along the interaural line and in the anterior–posterior direction. The results were then compared with our data on lower-lip mimetic Muscles and on the frontalis Muscle obtained in previous studies. Results Bilateral reproducible responses could be observed at coil positions varying from 1 to 13 cm lateral to the vertex. During moderate Muscle activation, maximum responses (mean amplitude 0.75 ± 0.44 mV contralateral, 0.74 ± 0.36 mV ipsilateral) were obtained at a mean stimulus position of 8.6 ± 1.6 cm lateral and 2.0 ± 2.2 cm anterior to the vertex for contralateral responses, and of 8.6 ± 2.0 cm lateral and 2.8 ± 2.4 cm anterior to the vertex for ipsilateral responses, respectively. Voluntary Muscle activation by forced eye-closure was associated with a further increase in mean amplitudes. At rest, bilateral responses could be elicited in 15 subjects (88.2%). During moderate Muscle activation, the shortest mean onset latencies were obtained at the optimum stimulus position on the interaural line, both for contralateral (10.2 ± 1.3 ms) and ipsilateral (10.6 ± 1.5 ms) MEPs. Comparing our data on the Orbicularis Oculi Muscle with those obtained on lower-lip Muscles and on the frontalis Muscle, there was a considerable overlap of coil positions from which reproducible MEPs could be elicited in all three groups of mimetic Muscles, but with the Orbicularis Oculi area being placed between forehead and lower-lip motorcortical areas. Conclusions A statistically significant separation of the cortical representation areas of forehead, Orbicularis oris, and lower-lip mimetic Muscles is possible by focal cortical TMS reflecting a kind of somatotopic organization of the face-associated motorcortex. Compared with the results on lower-lip and forehead Muscles, Orbicularis Oculi Muscle responses show characteristics of both.
Kei Yoneda - One of the best experts on this subject based on the ideXlab platform.
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reconstruction of the entire upper eyelid area with a subcutaneous pedicle flap based on the Orbicularis Oculi Muscle
Plastic and Reconstructive Surgery, 1991Co-Authors: Yohko Yoshimura, T Nakajima, Kei YonedaAbstract:: Lagophthalmos caused by trauma or inflammation may damage the cornea and, if not adequately treated, may result in loss of eyesight. Hence surgical intervention is required at the earliest possible stage. We have developed a subcutaneous pedicle flap elevated from the temporozygomatic area and rotated 180 degrees around a small pedicle base that contains the Orbicularis Oculi Muscle. With this flap, the entire upper eyelid can be reconstructed as an aesthetic unit in one stage so that the result is satisfactory not only functionally, but also cosmetically. The operative procedure is presented along with case reports.
Yipin Xu - One of the best experts on this subject based on the ideXlab platform.
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identification and characterization of skeletal Muscle stem cells from human Orbicularis Oculi Muscle
Tissue Engineering Part C-methods, 2018Co-Authors: Caihe Liao, Xi Chen, Yipin Xu, Xinhai YeAbstract:Skeletal Muscle stem cell (SMSC) transplantation has shown great therapeutical potential in repairing Muscle loss and dysfunction, but the Muscle acquisition is usually a traumatic procedure causing pain and morbidity to the donor. In this study, we investigated the feasibility of isolating SMSCs from human Orbicularis Oculi Muscle (OOM), which is routinely removed and discarded during ophthalmic cosmetic surgeries. OOM fragments were harvested from 18 female healthy donors undergoing upper eyelid plasties. Plastic-adherent cells were isolated from the Muscles using a two-step plating method combined with collagenase digestion. A total of 15 cell cultures were successfully established from the Muscle samples. These adherent cells were positive for the specific markers of SMSCs and could be directed toward the osteogenic, adipogenic, chondrogenic, and myogenic phenotypes in the presence of lineage-specific inductive media. Moreover, after cultured in the myogenic inductive medium for 3 weeks, the Muscle ce...
