The Experts below are selected from a list of 207 Experts worldwide ranked by ideXlab platform

Christoph Frohn - One of the best experts on this subject based on the ideXlab platform.

  • Kinetics and Organ Distribution of allogeneic natural killer lymphocytes transfused into patients suffering from renal cell carcinoma.
    Stem cells and development, 2004
    Co-Authors: Jörg-matthias Brand, Birgit Meller, K. Von Hof, J. Luhm, M. Bähre, Holger Kirchner, Christoph Frohn
    Abstract:

    The transfusion of natural killer (NK) lymphocytes into patients suffering from malignant diseases is an approach of current interest in the field of immunotherapy. Little is known about the Organ Distribution, survival, and clearance of donor immune effector cells in cellular therapy, and no reports exist on these important parameters considering NK cells in particular or any other type of allogeneic lymphocytes in humans. In the context of a clinical Phase I/II study we examined the Distribution of transfused allogeneic NK cells in patients suffering from renal cell carcinoma. The NK cells were ex vivo cultivated and activated before transfusion. To assess the circulation of the transfused cells in the peripheral blood, we used a nested PCR technique to detect HLA DRB1 alleles of the NK cell donors. Post-transfusion, all patients showed evidence of circulating donor cells for up to 3 days. After 7 days, all donor cells were cleared from the blood to undetectable levels. To assess Organ Distribution, 111...

  • Research Report Kinetics and Organ Distribution of Allogeneic Natural Killer Lymphocytes Transfused into Patients Suffering from Renal Cell Carcinoma
    2004
    Co-Authors: Birgit Meller, K. Von Hof, J. Luhm, M. Bähre, Holger Kirchner, Christoph Frohn
    Abstract:

    The transfusion of natural killer (NK) lymphocytes into patients suffering from malignant diseases is an approach of current interest in the field of immunotherapy. Little is known about the Organ Distribution, survival, and clearance of donor immune effector cells in cellular therapy, and no reports exist on these important parameters considering NK cells in particular or any other type of allogeneic lymphocytes in humans. In the context of a clinical Phase I/II study we examined the Distribution of transfused allogeneic NK cells in patients suffering from renal cell carcinoma. The NK cells were ex vivo cultivated and activated before transfusion. To assess the circulation of the transfused cells in the peripheral blood, we used a nested PCR technique to detect HLA DRB1 alleles of the NK cell donors. Post-transfusion, all patients showed evidence of circulating donor cells for up to 3 days. After 7 days, all donor cells were cleared from the blood to undetectable levels. To assess Organ Distribution, 111 In-labeled NK cells were injected and monitored by whole-body scintiscans. A Distribution to the whole body, with preference for liver, spleen, and bone marrow, was observed after a short initial uptake in the lungs. No activity was observed in lymphatic nodes. A total of 2/4 evaluable metastases showed a clear accumulation of transfused NK cells. The half-life corrected activity in all body compartments remained almost constant over the 6-day observation period in concordance with the absence of any excretion of radioactivity. This may indicate an extended survival of the transfused cells, despite their foreign nature, in the host Organism.

John Harris - One of the best experts on this subject based on the ideXlab platform.

  • trust and altruism Organ Distribution scandals do they provide good reasons to refuse posthumous donation
    Journal of Medicine and Philosophy, 2015
    Co-Authors: Annette Dufner, John Harris
    Abstract:

    A recent Organ Distribution scandal in Germany raises questions of general importance on which many thousands of lives may well depend. The scandal in Germany has produced reactions that are likely to occur whenever and wherever Distribution irregularities occur and become public knowledge. After it had become known that physicians in three German hospitals were in the habit of manipulating records in order to fast-track their patients' cases, the country experienced a decrease of available Organs by a staggering 40% in October 2012. Even though this loss of trust by donors and their families is understandable, and potentially a legitimate form of protest against wrongful Distribution, the withdrawal of agreement to serve as a posthumous donor in response to irregularities also inevitably results in avoidable poor outcomes for highly vulnerable individuals. In this paper, we provide a moral analysis of such dilemmas and make recommendations as to the way forward.

  • Trust and Altruism—Organ Distribution Scandals: Do They Provide Good Reasons to Refuse Posthumous Donation?
    The Journal of medicine and philosophy, 2015
    Co-Authors: Annette Dufner, John Harris
    Abstract:

    A recent Organ Distribution scandal in Germany raises questions of general importance on which many thousands of lives may well depend. The scandal in Germany has produced reactions that are likely to occur whenever and wherever Distribution irregularities occur and become public knowledge. After it had become known that physicians in three German hospitals were in the habit of manipulating records in order to fast-track their patients' cases, the country experienced a decrease of available Organs by a staggering 40% in October 2012. Even though this loss of trust by donors and their families is understandable, and potentially a legitimate form of protest against wrongful Distribution, the withdrawal of agreement to serve as a posthumous donor in response to irregularities also inevitably results in avoidable poor outcomes for highly vulnerable individuals. In this paper, we provide a moral analysis of such dilemmas and make recommendations as to the way forward.

Jörg Kreuter - One of the best experts on this subject based on the ideXlab platform.

Fang Peng - One of the best experts on this subject based on the ideXlab platform.

  • 18 F-fluorodeoxyglucose positron emission tomography–computed tomography for assessing Organ Distribution of stressed red blood cells in mice
    Scientific reports, 2021
    Co-Authors: Wen-yu Yin, Jiao Yuan, Zhi-min Zhang, Cheng Mei, Yong-xiang Tang, Fang Peng
    Abstract:

    Red blood cells (RBCs) stressed by high temperature are similar to senescent or damaged RBCs in pathological conditions. RBCs can be efficiently labelled with 18F-fluorodeoxyglucose (FDG). The aim of this study was to assess stressed RBCs erythrophagocytosis and Organ Distribution in vivo with the application of 18F-FDG PET/CT. RBCs were induced under high temperature (48 °C) to prepare stressed RBCs. Fluorescence-activated cell sorting (FACS) was used to analyse reactive oxygen species (ROS) generation, intracellular Ca2+ concentration and membrane phosphatidylserine (PS) externalization of RBCs. 18F-FDG was used to label RBCs and assess the erythrophagocytosis. Finally, 18F-FDG PET/CT was applied to reveal and measure the Organ Distribution of stressed RBCs in mice. Compared with untreated RBCs, stressed RBCs decreased in cell volume and increased in ROS level, intracellular Ca2+ concentration, and PS exposure. RBCs could be labelled by 18F-FDG. Stressed RBCs tended to be phagocytosed by macrophages via assessment of FACS and radioactivity. 18F-FDG PET/CT imaging showed that stressed RBCs were mainly trapped in spleen, while untreated RBCs remained in circulation system. Thus, stressed RBCs can be effectively labelled by 18F-FDG and tend to be trapped in spleen of mice as assessed by PET/CT.

Annette Dufner - One of the best experts on this subject based on the ideXlab platform.

  • trust and altruism Organ Distribution scandals do they provide good reasons to refuse posthumous donation
    Journal of Medicine and Philosophy, 2015
    Co-Authors: Annette Dufner, John Harris
    Abstract:

    A recent Organ Distribution scandal in Germany raises questions of general importance on which many thousands of lives may well depend. The scandal in Germany has produced reactions that are likely to occur whenever and wherever Distribution irregularities occur and become public knowledge. After it had become known that physicians in three German hospitals were in the habit of manipulating records in order to fast-track their patients' cases, the country experienced a decrease of available Organs by a staggering 40% in October 2012. Even though this loss of trust by donors and their families is understandable, and potentially a legitimate form of protest against wrongful Distribution, the withdrawal of agreement to serve as a posthumous donor in response to irregularities also inevitably results in avoidable poor outcomes for highly vulnerable individuals. In this paper, we provide a moral analysis of such dilemmas and make recommendations as to the way forward.

  • Trust and Altruism—Organ Distribution Scandals: Do They Provide Good Reasons to Refuse Posthumous Donation?
    The Journal of medicine and philosophy, 2015
    Co-Authors: Annette Dufner, John Harris
    Abstract:

    A recent Organ Distribution scandal in Germany raises questions of general importance on which many thousands of lives may well depend. The scandal in Germany has produced reactions that are likely to occur whenever and wherever Distribution irregularities occur and become public knowledge. After it had become known that physicians in three German hospitals were in the habit of manipulating records in order to fast-track their patients' cases, the country experienced a decrease of available Organs by a staggering 40% in October 2012. Even though this loss of trust by donors and their families is understandable, and potentially a legitimate form of protest against wrongful Distribution, the withdrawal of agreement to serve as a posthumous donor in response to irregularities also inevitably results in avoidable poor outcomes for highly vulnerable individuals. In this paper, we provide a moral analysis of such dilemmas and make recommendations as to the way forward.