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Asta Judzentiene - One of the best experts on this subject based on the ideXlab platform.
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The essential oil of Origanum Vulgare L. ssp. Vulgare growing wild in Vilnius district (Lithuania)
Phytochemistry, 2001Co-Authors: Danute Mockute, Genovaite Bernotiene, Asta JudzentieneAbstract:Abstract The plants of wild Origanum Vulgare L. ssp. Vulgare were collected in 10 localities of Vilnius district (Lithuania) in 1995–1999. The main constituents of the essential oils from 8 localities were β-ocimene (14.9–21.6%), germacrene D (10.0–16.2 ), β-caryophyllene (10.8–15.7%) and sabinene (6.6–14.2%). The essential oils from two localities contained only three above compounds as major components: germacrene D, β-ocimene and sabinene or β-caryophyllene, β–ocimene and germacrene D. Three chemotypes of essential oils were identified. The main chemotype was β-ocimene–germacrene D–β-caryophyllene. The terpenic hydrocarbons made up 52.8–80.6% of the essential oils. The 42 identified components made up 85.6–98.0% of the essential oil.
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The essential oil of Origanum Vulgare L. ssp. Vulgare growing wild in vilnius district (Lithuania).
Phytochemistry, 2001Co-Authors: Danute Mockute, Genovaite Bernotiene, Asta JudzentieneAbstract:The plants of wild Origanum Vulgare L. ssp. Vulgare were collected in 10 localities of Vilnius district (Lithuania) in 1995-1999. The main constituents of the essential oils from 8 localities were beta-ocimene (14.9-21.6%), germacrene D (10.0-16.2), beta-caryophyllene (10.8- 15.7%) and sabinene (6.6- 4.2%). The essential oils from two localities contained only three above compounds as major components: germacrene D, beta-ocimene and sabinene or beta-caryophyllene, beta-ocimene and germacrene D. Three chemotypes of essential oils were identified. The main chemotype was beta-ocimene germacrene D-beta-caryophyllene. The terpenic hydrocarbons made up 52.8-80.6% of the essential oils. The 42 identified components made up 85.6-98.0% of the essential oil.
Albines Nizama, William Alonso - One of the best experts on this subject based on the ideXlab platform.
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Efecto antibacteriano in vitro del syzygium aromaticum "clavo de olor" y Origanum Vulgare "orégano" frente a streptococcus muntans atcc 25175
Universidad Privada Antenor Orrego - UPAO, 2020Co-Authors: Albines Nizama, William AlonsoAbstract:Evaluar el efecto antibacteriano in vitro del syzygium aromaticum “clavo de olor” y Origanum Vulgare “orégano” frente al streptococcus mutans ATCC 25175. Material y método: El presente estudio de corte transversal, comparativo, se realizó mediante el método de difusión en agar con disco de Kirby – Bauer para evaluar la sensibilidad bacteriana. Se obtuvieron aceites esenciales de orégano y clavo de olor de la empresa de aromaterapia Ekala, ambos a una concentración del 100%. Asimismo, se consiguió la cepa de Streptococcus mutans ATCC 25175 del laboratorio GenLab del Perú Sac; donde cada cepa se cultivó en placas Petri en agar Müller Hinton y se colocaron discos de papel filtro embebidos con aceites esenciales de clavo de olor y orégano a una concentración del 100% cada una. Estos aceites esenciales fueron comparados con Gluconato de clorhexidina al 0.12% como grupo control. Posteriormente, se incubaron a 37° C por 24 horas, para luego realizar la medición del diámetro del halo de inhibición. Para la confiabilidad de este estudio, se utilizó el método estadístico Kappa. Para el análisis de resultados se empleó el programa estadístico SPSS versión 23 y Excel, para luego presentar los resultados en tablas y gráficos. Resultados: Al evaluar el efecto antibacteriano in vitro del Syzygium aromaticum “clavo de olor” y Origanum Vulgare “orégano” frente al Streptococcus mutans ATCC 25175, se observó que el halo de inhibición del Syzygium aromaticum (100%) tuvo un promedio de 20.09 mm ± 1.87mm, mientras que el halo de inhibición del Origanum Vulgare tuvo un promedio de 14.74 mm ± 0.41. Conclusiones: Se concluye que el mayor promedio fue del Syzygium aromaticum (100%) con un halo de 20.09mm y el menor promedio fue para el Gluconato de Clorhexidina (0,12%) con un halo de 10.63mm.To evaluate the in vitro antibacterial effect of Syzygium aromaticum “clove” and Origanum Vulgare “oregano” against Streptococcus mutans ATCC 25175. Material and method: This comparative cross-sectional study was carried out using the Kirby-Bauer disc agar diffusion method to evaluate bacterial sensitivity. Essential oils of oregano and clove were obtained from the aromatherapy company Ekala, both at a concentration of 100%. Likewise, the Streptococcus mutans ATCC 25175 strain was obtained from the GenLab laboratory in Peru Sac; where each strain was grown in Petri dishes in Müller Hinton agar and filter paper discs embedded with essential oils of clove and oregano were placed at a concentration of 100% each. These essential oils were compared with 0.12% chlorhexidine gluconate as a control group. Subsequently, they were incubated at 37 ° C for 24 hours, and then measured the diameter of the inhibition halo. For the reliabil ity of this study, the Kappa statistical method was used. For the analysis of results, the statistical program SPSS version 23 and Excel was used, to then present the results in tables and graphs. Results: When evaluating the in vitro antibacterial effect of Syzygium aromaticum ""clove"" and Origanum Vulgare ""oregano"" against Streptococcus mutans ATCC 25175, it was observed that the inhibition halo of Syzygium aromaticum (100%) had an average of 20.09 mm ± 1.87 mm, while the Origanum Vulgare inhibition halo averaged 14.74 mm ± 0.41. Conclusions: It is concluded that the highest average was Syzygium aromaticum (100%) with a halo of 20.09mm and the lowest average was for Chlorhexidine Gluconate (0.12%) with a halo of 10.63mm.Tesi
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Efecto antibacteriano in vitro del SYZYGIUM AROMATICUM "clavo de olor" y Origanum Vulgare "oregano" frente a STREPTOCOCCUS MUNTANS TCC 25175
Universidad Privada Antenor Orrego - UPAO, 2020Co-Authors: Albines Nizama, William AlonsoAbstract:Objetivo: Evaluar el efecto antibacteriano in vitro del syzygium aromaticum “clavo de olor” y Origanum Vulgare “orégano” frente al streptococcus mutans ATCC 25175. Material y método: El presente estudio de corte transversal, comparativo, se realizó mediante el método de difusión en agar con disco de Kirby – Bauer para evaluar la sensibilidad bacteriana. Se obtuvieron aceites esenciales de orégano y clavo de olor de la empresa de aromaterapia Ekala, ambos a una concentración del 100%. Asimismo, se consiguió la cepa de Streptococcus mutans ATCC 25175 del laboratorio GenLab del Perú Sac; donde cada cepa se cultivó en placas Petri en agar Müller Hinton y se colocaron discos de papel filtro embebidos con aceites esenciales de clavo de olor y orégano a una concentración del 100% cada una. Estos aceites esenciales fueron comparados con Gluconato de clorhexidina al 0.12% como grupo control. Posteriormente, se incubaron a 37° C por 24 horas, para luego realizar la medición del diámetro del halo de inhibición. Para la confiabilidad de este estudio, se utilizó el método estadístico Kappa. Para el análisis de resultados se empleó el programa estadístico SPSS versión 23 y Excel, para luego presentar los resultados en tablas y gráficos. Resultados: Al evaluar el efecto antibacteriano in vitro del Syzygium aromaticum “clavo de olor” y Origanum Vulgare “orégano” frente al Streptococcus mutans ATCC 25175, se observó que el halo de inhibición del Syzygium aromaticum (100%) tuvo un promedio de 20.09 mm ± 1.87mm, mientras que el halo de inhibición del Origanum Vulgare tuvo un promedio de 14.74 mm ± 0.41. Conclusiones: Se concluye que el mayor promedio fue del Syzygium aromaticum (100%) con un halo de 20.09mm y el menor promedio fue para el Gluconato de Clorhexidina (0,12%) con un halo de 10.63mm.Objective: To evaluate the in vitro antibacterial effect of Syzygium aromaticum “clove” and Origanum Vulgare “oregano” against Streptococcus mutans ATCC 25175. Material and method: This comparative cross-sectional study was carried out using the Kirby-Bauer disc agar diffusion method to evaluate bacterial sensitivity. Essential oils of oregano and clove were obtained from the aromatherapy company Ekala, both at a concentration of 100%. Likewise, the Streptococcus mutans ATCC 25175 strain was obtained from the GenLab laboratory in Peru Sac; where each strain was grown in Petri dishes in Müller Hinton agar and filter paper discs embedded with essential oils of clove and oregano were placed at a concentration of 100% each. These essential oils were compared with 0.12% chlorhexidine gluconate as a control group. Subsequently, they were incubated at 37 ° C for 24 hours, and then measured the diameter of the inhibition halo. For the reliabil ity of this study, the Kappa statistical method was used. For the analysis of results, the statistical program SPSS version 23 and Excel was used, to then present the results in tables and graphs. Results: When evaluating the in vitro antibacterial effect of Syzygium aromaticum ""clove"" and Origanum Vulgare ""oregano"" against Streptococcus mutans ATCC 25175, it was observed that the inhibition halo of Syzygium aromaticum (100%) had an average of 20.09 mm ± 1.87 mm, while the Origanum Vulgare inhibition halo averaged 14.74 mm ± 0.41. Conclusions: It is concluded that the highest average was Syzygium aromaticum (100%) with a halo of 20.09mm and the lowest average was for Chlorhexidine Gluconate (0.12%) with a halo of 10.63mm.Tesi
Danute Mockute - One of the best experts on this subject based on the ideXlab platform.
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The essential oil of Origanum Vulgare L. ssp. Vulgare growing wild in Vilnius district (Lithuania)
Phytochemistry, 2001Co-Authors: Danute Mockute, Genovaite Bernotiene, Asta JudzentieneAbstract:Abstract The plants of wild Origanum Vulgare L. ssp. Vulgare were collected in 10 localities of Vilnius district (Lithuania) in 1995–1999. The main constituents of the essential oils from 8 localities were β-ocimene (14.9–21.6%), germacrene D (10.0–16.2 ), β-caryophyllene (10.8–15.7%) and sabinene (6.6–14.2%). The essential oils from two localities contained only three above compounds as major components: germacrene D, β-ocimene and sabinene or β-caryophyllene, β–ocimene and germacrene D. Three chemotypes of essential oils were identified. The main chemotype was β-ocimene–germacrene D–β-caryophyllene. The terpenic hydrocarbons made up 52.8–80.6% of the essential oils. The 42 identified components made up 85.6–98.0% of the essential oil.
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The essential oil of Origanum Vulgare L. ssp. Vulgare growing wild in vilnius district (Lithuania).
Phytochemistry, 2001Co-Authors: Danute Mockute, Genovaite Bernotiene, Asta JudzentieneAbstract:The plants of wild Origanum Vulgare L. ssp. Vulgare were collected in 10 localities of Vilnius district (Lithuania) in 1995-1999. The main constituents of the essential oils from 8 localities were beta-ocimene (14.9-21.6%), germacrene D (10.0-16.2), beta-caryophyllene (10.8- 15.7%) and sabinene (6.6- 4.2%). The essential oils from two localities contained only three above compounds as major components: germacrene D, beta-ocimene and sabinene or beta-caryophyllene, beta-ocimene and germacrene D. Three chemotypes of essential oils were identified. The main chemotype was beta-ocimene germacrene D-beta-caryophyllene. The terpenic hydrocarbons made up 52.8-80.6% of the essential oils. The 42 identified components made up 85.6-98.0% of the essential oil.
Durango-chávez, Olga Karina - One of the best experts on this subject based on the ideXlab platform.
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Comparación del efecto in vitro de los aceites esenciales de Eucalyptus globulus y Origanum Vulgare sobre Candida albicans aislada de paciente con candidiasis vulvovaginal
'Universidad Nacional de Tujillo', 2020Co-Authors: Durango-chávez, Olga Karina, Mejía-delgado, Elva ManuelaAbstract:Objetivo:Comparar el efecto in vitro del aceite esencial de Eucalyptus globulus con el aceite esencial de Origanum Vulgare sobre el crecimiento de Candida albicans aislada de paciente con candidiasis vulvovaginal. Material y métodos:Candida albicans aislada de paciente con candidiasis vulvovaginal para las pruebas in vitro.La actividad antifúngica se determinó por el método Kirby y Bauer o de difusión de discos; la concentración inhibitoria mínima (CIM) y la concentración mínima fungicida (CMF) mediante el conteo de las unidades formadoras de colonias (UFC). Principales medidas de resultados: Halos de inhibición, CIM, CMF. Resultados: se determinó que la diferencia de las medias entre los diámetros de los halos de inhibición de las concentraciones al 25%, 50%, 75% y 100% del aceite esencial de Eucalyptus globulus y del aceite esencial de Origanum Vulgare es significativa, ya que p valor .0000, siendo menor que el nivel de significancia (p < 0.05), con lo que se determinó que el primero presentó menor efecto inhibitorio que el segundo. Se determinó que la CMI del aceite esencial de Eucalyptus globulus fue de 50% y del aceite esencial de Origanum Vulgare fue de 25%, en los cuales, se observó ausencia de turbidez. Se observó que solamente el aceite esencial de Origanum Vulgare presentó a partir de la concentración 75% (UFC = 0), el cual fue considerado como la concentración mínima fungicida (CMF). Conclusiones: El aceite esencial de Eucalyptus globulus presenta menor efecto inhibitorio que el aceite esencial de Origanum Vulgare sobre el crecimiento de Candida albicans aislada de paciente con candidiasis vulvovaginal en condiciones in vitro.Palabras Clave:Candida albicans; Eucalyptus globulus; Origanum Vulgare.DOI: http://dx.doi.org/10.17268/rmt.2020.v15i01.0
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Comparación del efecto in vitro de los aceites esenciales de eucalyptus globulus y Origanum Vulgare sobre candida albicans aislada de paciente con candidiasis vulvovaginal
Universidad Privada Antenor Orrego - UPAO, 2020Co-Authors: Durango-chávez, Olga KarinaAbstract:Comparación del efecto in vitro de los aceites esenciales de Eucalyptus globulus y Origanum Vulgare sobre Candida albicans aislada de paciente con candidiasis vulvovaginal Objetivo: Comparar el efecto in vitro del aceite esencial de Eucalyptus globulus con el aceite esencial de Origanum Vulgare sobre el crecimiento de Candida albicans aislada de paciente con candidiasis vulvovaginal. Material y métodos: Candida albicans aislada de paciente con candidiasis vulvovaginal para las pruebas in vitro. La actividad antifúngica se determinó por el método Kirby y Bauer o de difusión de discos; la concentración inhibitoria mínima (CIM) y la concentración mínima fungicida (CMF) mediante el conteo de las unidades formadoras de colonias (UFC). Principales medidas de resultados: Halos de inhibición, CIM, CMF. Resultados: A través de la prueba no paramétrica de W de Wilcoxon, se determinó que la diferencia de las medias entre los diámetros de los halos de inhibición de las concentraciones al 25%, 50%, 75% y 100% del aceite esencial de Eucalyptus globulus y del aceite esencial de Origanum Vulgare es significativa, ya que p valor .0000, siendo menor que el nivel de significancia (p < 0.05), con lo que se determinó que el primero presentó menor efecto inhibitorio que el segundo. Se determinó que la CMI del aceite esencial de Eucalyptus globulus fue de 50% y del aceite esencial de Origanum Vulgare fue de 25%, en los cuales, se observó ausencia de turbidez. Se observó que solamente el aceite esencial de Origanum Vulgare presentó a partir de la concentración 75% (UFC = 0), el cual fue considerado como la concentración mínima fungicida (CMF). Conclusiones: El aceite esencial de Eucalyptus globulus presenta menor efecto inhibitorio que el aceite esencial de Origanum Vulgare sobre el crecimiento de Candida albicans aislada de paciente con candidiasis vulvovaginal en condiciones in vitro.Comparison of the in vitro effect of the essential oils of Eucalyptus globulus and Origanum Vulgare in Candida albicans isolated from patient with vulvovaginal candidiasis Objective: To compare the in vitro effect of Eucalyptus globulus essential oil with the essential oil of Origanum Vulgare on the growth of Candida albicans isolated from a patient with vulvovaginal candidiasis. Material and Methods: Candida albicans isolated from patient with vulvovaginal candidiasis for in vitro study. The antifungal activity was determined by the Kirby and Bauer method or disc diffusion; the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) by counting the colony forming units (CFU). Main outcome measures: Inhibition halos, MIC, MFC. Results: Through the Wilcoxon W nonparametric test, it was determined that the difference of the means between the diameters of the inhibition haloes of the concentrations of 25%, 50%, 75% and 100% of the essential oil of Eucalyptus globulus and of the essential oil of Origanum Vulgare is significant, since p value .0000, being lower than the level of significance (p
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Comparación del efecto in vitro de los aceites esenciales de eucalyptus globulus y Origanum Vulgare sobre candida albicans aislada de paciente con candidiasis vulvovaginal
Universidad Privada Antenor Orrego - UPAO, 2020Co-Authors: Durango-chávez, Olga KarinaAbstract:Comparison of the in vitro effect of the essential oils of Eucalyptus globulus and Origanum Vulgare in Candida albicans isolated from patient with vulvovaginal candidiasis Objective: To compare the in vitro effect of Eucalyptus globulus essential oil with the essential oil of Origanum Vulgare on the growth of Candida albicans isolated from a patient with vulvovaginal candidiasis. Material and Methods: Candida albicans isolated from patient with vulvovaginal candidiasis for in vitro study. The antifungal activity was determined by the Kirby and Bauer method or disc diffusion; the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) by counting the colony forming units (CFU). Main outcome measures: Inhibition halos, MIC, MFC. Results: Through the Wilcoxon W nonparametric test, it was determined that the difference of the means between the diameters of the inhibition haloes of the concentrations of 25%, 50%, 75% and 100% of the essential oil of Eucalyptus globulus and of the essential oil of Origanum Vulgare is significant, since p value .0000, being lower than the level of significance (p
Genovaite Bernotiene - One of the best experts on this subject based on the ideXlab platform.
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The essential oil of Origanum Vulgare L. ssp. Vulgare growing wild in Vilnius district (Lithuania)
Phytochemistry, 2001Co-Authors: Danute Mockute, Genovaite Bernotiene, Asta JudzentieneAbstract:Abstract The plants of wild Origanum Vulgare L. ssp. Vulgare were collected in 10 localities of Vilnius district (Lithuania) in 1995–1999. The main constituents of the essential oils from 8 localities were β-ocimene (14.9–21.6%), germacrene D (10.0–16.2 ), β-caryophyllene (10.8–15.7%) and sabinene (6.6–14.2%). The essential oils from two localities contained only three above compounds as major components: germacrene D, β-ocimene and sabinene or β-caryophyllene, β–ocimene and germacrene D. Three chemotypes of essential oils were identified. The main chemotype was β-ocimene–germacrene D–β-caryophyllene. The terpenic hydrocarbons made up 52.8–80.6% of the essential oils. The 42 identified components made up 85.6–98.0% of the essential oil.
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The essential oil of Origanum Vulgare L. ssp. Vulgare growing wild in vilnius district (Lithuania).
Phytochemistry, 2001Co-Authors: Danute Mockute, Genovaite Bernotiene, Asta JudzentieneAbstract:The plants of wild Origanum Vulgare L. ssp. Vulgare were collected in 10 localities of Vilnius district (Lithuania) in 1995-1999. The main constituents of the essential oils from 8 localities were beta-ocimene (14.9-21.6%), germacrene D (10.0-16.2), beta-caryophyllene (10.8- 15.7%) and sabinene (6.6- 4.2%). The essential oils from two localities contained only three above compounds as major components: germacrene D, beta-ocimene and sabinene or beta-caryophyllene, beta-ocimene and germacrene D. Three chemotypes of essential oils were identified. The main chemotype was beta-ocimene germacrene D-beta-caryophyllene. The terpenic hydrocarbons made up 52.8-80.6% of the essential oils. The 42 identified components made up 85.6-98.0% of the essential oil.