The Experts below are selected from a list of 78 Experts worldwide ranked by ideXlab platform
Ney Rômulo De Oliveira Paula - One of the best experts on this subject based on the ideXlab platform.
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Serological investigation of lentiviruses of small ruminants in the microregions of Alto Médio Canindé, Picos and Floriano, Piauí state, Brazil
2018Co-Authors: Ricardo Abílio Bezerra E Silva, Maria Do Carmo De Souza Batista, Raíssa Paula Araújo Alves, Francisco Selmo Fernandes Alves, Raymundo Rizaldo Pinheiro, Bruno Leandro Maranhão Diniz, Wagner Martins Fontes Do Rêgo, Janaina De Fátima Saraiva Cardoso, Ney Rômulo De Oliveira PaulaAbstract:ABSTRACT: Small ruminant lentiviruses (LV) is the generic term for the caprine arthritis-encephalitis and Maedi-Visna viruses, which belong to the Retroviridae family, Orthoretrovirinae subfamily, Lentivirus genus. The virus infects goats and sheep, causing slow course of disease with inflammatory, chronic and degenerative lesions, which can reach several organs, provoking cachexia and death. Infected animals eliminate the virus mainly through secretions and excretions and transmit it especially in close contact situations. There is no treatment to date. The control is based on segregated creation, management and sacrifice of the positive. This infectious agent has been reported in various parts of the world and is responsible for significant economic losses. It was verified in several states of Brazil and there are seroepidemiological data in southeast and southwest mesoregions of Piauí, Brazil. This research aimed to perform serological survey to investigate the occurrence of antibodies to LV in sheep and goats, in the regions of Alto Médio Canindé, Picos and Floriano. So, 1,280 and 1,360 serum goats and sheep samples, respectively, were collected, coming from 20 municipalities, distributed in the 3 microregions. The number of samples was proportional to the actual herd of each municipality. The samples were analyzed using the agar gel immunodiffusion test. None of the surveyed sera reacted positively, though there is a null seroprevalence. It was emphasized the importance of implementing a rigorous control program in order to prevent the introduction and spread of this infectious agent in these microregions.
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Investigação sorológica das lentiviroses de pequenos ruminantes nas microrregiões homogêneas do Alto Médio Canindé, Picos e Floriano, Piauí, Brasil
Zeppelini Editorial, 2017Co-Authors: Ricardo Abílio Bezerra E Silva, Maria Do Carmo De Souza Batista, Raíssa Paula Araújo Alves, Francisco Selmo Fernandes Alves, Raymundo Rizaldo Pinheiro, Bruno Leandro Maranhão Diniz, Wagner Martins Fontes Do Rêgo, Janaina De Fátima Saraiva Cardoso, Ney Rômulo De Oliveira PaulaAbstract:RESUMO: Lentivírus de pequenos ruminantes (LV) é o termo genérico utilizado para designar os vírus da artrite encefalite caprina e Maedi-Visna, os quais pertencem à família Retroviridae, subfamília Orthoretrovirinae, gênero Lentivirus. Tais vírus infectam caprinos e ovinos, causando enfermidades de curso lento com lesões inflamatórias, crônicas e degenerativas que podem atingir vários órgãos, provocando caquexia e morte. Os animais infectados eliminam o vírus sobretudo por meio de secreções e excreções e transmitem-no especialmente em situações de estreito contato. Não há tratamento até o momento. O controle é baseado na criação segregada, no manejo e no sacrifício dos positivos. Esse agente infeccioso já foi relatado em várias partes do mundo, sendo responsável por perdas econômicas significativas. Por o agente ter sido verificado em vários estados do Brasil e por não existirem dados soroepidemiológicos nas mesorregiões sudeste e sudoeste piauiense, esta pesquisa teve por objetivo realizar inquérito sorológico para investigar a ocorrência de anticorpos para o LV em ovinos e caprinos nas microrregiões do Alto Médio Canindé, Picos e Floriano, no Piauí. Para tanto, foram coletadas 1.280 e 1.360 amostras de soro caprino e ovino, respectivamente, oriundos de 20 municípios, distribuídos nas três microrregiões, sendo o número de amostras proporcional ao rebanho efetivo de cada município. As amostras de soro foram analisadas utilizando o teste de imunodifusão em gel de agarose (IDGA). Nenhum dos soros pesquisados reagiu positivamente, constatando-se soroprevalência nula. Ressalta-se a importância da implantação de um rigoroso programa de controle para que se possa evitar a introdução e/ou a disseminação desse agente infeccioso nessas microrregiões
G G Ananko - One of the best experts on this subject based on the ideXlab platform.
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antiviral activity of extracts of basidiomycetes and humic compounds substances against human immunodeficiency virus retroviridae Orthoretrovirinae lentivirus human immunodeficiency virus 1 and herpes simplex virus herpesviridae simplexvirus human al
Voprosy virusologii, 2020Co-Authors: D N Nosik, N N Nosik, T V Teplyakova, O A Lobach, I A Kiseleva, N G Kondrashina, M S Bochkova, G G AnankoAbstract:Introduction . One of the most urgent problem of modern medicine is the fight against the disease caused by the Human Immunodeficiency Virus (HIV) – HIV infection. The chemical compounds have improved the situation for infected people, but they are toxic, disrupt the metabolism and cannot eliminate the integrated virus from the body. The emergence of resistant HIV strains makes these treatments ineffective. Often, the death of HIV-infected people occurs as a result of the development of opportunistic infections caused by viruses of the Herpesviridae family. Therefore, the search for new therapeutic and preventive drugs that are less toxic and active against several viruses at the same time is relevant. Basidiomycetes , higher fungi, are a source of medicinal compounds that have antimicrobial properties, as well as antiviral ones. Humic compounds (HS) of various nature also have antiviral activity. The aim of the study was to obtain nontoxic compounds from the basidiomycete Inonotus obliquus and humic compounds from brown coals and to test their activity against viruses that are pathogenic to humans: HIV and Herpes Simplex Virus (HSV). Material and methods . The antiviral activity of melanin extracts obtained from the culture of the chaga fungus Inonotus obliquus and HS from the brown coal of the Kansko-Achinsk Deposit was studied using a model of MT-4 lymphoblastoid cells infected with HIV type 1 (HIV–1) strains and a monolayer culture of Vero cells infected with HSV type 1 (HSV-1) using virological and statistical research methods. Results and discussion . It was found that all the studied compounds did not have a cytotoxic effect on cells at a concentration of 100 mcg / ml. It was shown that extracts of basidiomycetes and HS have antiviral activity against HIV-1 and HSV-1. EC 50 (50%-effective concentration) for HIV-1 was 3.7–5.0 mcg/ml, selectivity index 28–35. Antiherpetic activity was detected at a dose of 50–100 mcg/ml. The antiviral effectiveness of melanin compounds was established both in the «preventive» (2 hours before cell infection) and in the «therapeutic» regimen of drug administration, both for HIV-1 and HSV-1. The presence of antiviral activity of melanin and HS in relation to the RNA-containing HIV-1 virus and DNA-containing HSV-1 virus in our study coincides with the results of a number of authors in relation to influenza viruses, herpes virus, HIV, hepatitis B virus, Coxsackievirus, smallpox vaccine virus, which suggests that the type of nucleic acid in the virus does not play a fundamental role in the antiviral action of these drugs. It is also clear that HS is effective against both enveloped and non-enveloped viruses. Conclusion . In general, it can be concluded that melanin and humic compounds are characterized by low toxicity in the presence of both virucidal and antiviral activity. This allows us to consider the studied compounds as the basis for creating safe medicines that are effective against pathogens of various viral infections.
Ricardo Abílio Bezerra E Silva - One of the best experts on this subject based on the ideXlab platform.
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Serological investigation of lentiviruses of small ruminants in the microregions of Alto Médio Canindé, Picos and Floriano, Piauí state, Brazil
2018Co-Authors: Ricardo Abílio Bezerra E Silva, Maria Do Carmo De Souza Batista, Raíssa Paula Araújo Alves, Francisco Selmo Fernandes Alves, Raymundo Rizaldo Pinheiro, Bruno Leandro Maranhão Diniz, Wagner Martins Fontes Do Rêgo, Janaina De Fátima Saraiva Cardoso, Ney Rômulo De Oliveira PaulaAbstract:ABSTRACT: Small ruminant lentiviruses (LV) is the generic term for the caprine arthritis-encephalitis and Maedi-Visna viruses, which belong to the Retroviridae family, Orthoretrovirinae subfamily, Lentivirus genus. The virus infects goats and sheep, causing slow course of disease with inflammatory, chronic and degenerative lesions, which can reach several organs, provoking cachexia and death. Infected animals eliminate the virus mainly through secretions and excretions and transmit it especially in close contact situations. There is no treatment to date. The control is based on segregated creation, management and sacrifice of the positive. This infectious agent has been reported in various parts of the world and is responsible for significant economic losses. It was verified in several states of Brazil and there are seroepidemiological data in southeast and southwest mesoregions of Piauí, Brazil. This research aimed to perform serological survey to investigate the occurrence of antibodies to LV in sheep and goats, in the regions of Alto Médio Canindé, Picos and Floriano. So, 1,280 and 1,360 serum goats and sheep samples, respectively, were collected, coming from 20 municipalities, distributed in the 3 microregions. The number of samples was proportional to the actual herd of each municipality. The samples were analyzed using the agar gel immunodiffusion test. None of the surveyed sera reacted positively, though there is a null seroprevalence. It was emphasized the importance of implementing a rigorous control program in order to prevent the introduction and spread of this infectious agent in these microregions.
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Investigação sorológica das lentiviroses de pequenos ruminantes nas microrregiões homogêneas do Alto Médio Canindé, Picos e Floriano, Piauí, Brasil
Zeppelini Editorial, 2017Co-Authors: Ricardo Abílio Bezerra E Silva, Maria Do Carmo De Souza Batista, Raíssa Paula Araújo Alves, Francisco Selmo Fernandes Alves, Raymundo Rizaldo Pinheiro, Bruno Leandro Maranhão Diniz, Wagner Martins Fontes Do Rêgo, Janaina De Fátima Saraiva Cardoso, Ney Rômulo De Oliveira PaulaAbstract:RESUMO: Lentivírus de pequenos ruminantes (LV) é o termo genérico utilizado para designar os vírus da artrite encefalite caprina e Maedi-Visna, os quais pertencem à família Retroviridae, subfamília Orthoretrovirinae, gênero Lentivirus. Tais vírus infectam caprinos e ovinos, causando enfermidades de curso lento com lesões inflamatórias, crônicas e degenerativas que podem atingir vários órgãos, provocando caquexia e morte. Os animais infectados eliminam o vírus sobretudo por meio de secreções e excreções e transmitem-no especialmente em situações de estreito contato. Não há tratamento até o momento. O controle é baseado na criação segregada, no manejo e no sacrifício dos positivos. Esse agente infeccioso já foi relatado em várias partes do mundo, sendo responsável por perdas econômicas significativas. Por o agente ter sido verificado em vários estados do Brasil e por não existirem dados soroepidemiológicos nas mesorregiões sudeste e sudoeste piauiense, esta pesquisa teve por objetivo realizar inquérito sorológico para investigar a ocorrência de anticorpos para o LV em ovinos e caprinos nas microrregiões do Alto Médio Canindé, Picos e Floriano, no Piauí. Para tanto, foram coletadas 1.280 e 1.360 amostras de soro caprino e ovino, respectivamente, oriundos de 20 municípios, distribuídos nas três microrregiões, sendo o número de amostras proporcional ao rebanho efetivo de cada município. As amostras de soro foram analisadas utilizando o teste de imunodifusão em gel de agarose (IDGA). Nenhum dos soros pesquisados reagiu positivamente, constatando-se soroprevalência nula. Ressalta-se a importância da implantação de um rigoroso programa de controle para que se possa evitar a introdução e/ou a disseminação desse agente infeccioso nessas microrregiões
Yu V Balabanyan - One of the best experts on this subject based on the ideXlab platform.
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study of the specific toxic effects of the substance 1 2 2 benzoylphenoxy ethyl 6 methyluracil the original non nucleoside inhibitor of human immunodeficiency virus type 1 retroviridae Orthoretrovirinae lentivirus human immunodeficiency virus 1 reverse transcriptase
Voprosy virusologii, 2021Co-Authors: E A Gaidai, K L Kryshen, E Jain A Korsakova, D V Demchenko, D R Kargopoltseva, A E Katelnikova, D S Gaidai, Yu V BalabanyanAbstract:Introduction. Combination antiretroviral therapy is currently the main component of treatment for human immunodeficiency virus (HIV) infected patients. At the same time, the high mutational potential of the virus and the frequency of side effects of existing drugs dictate the need for the development and preclinical study of new, more effective and safer compounds. The aim of the study is to evaluate the specific types of toxicity of a new non-nucleoside inhibitor of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RNA-dependent DNA revertase) (NNRTI) based on the substance 1-[2-(2-benzoylphenoxy)ethyl]-6-methyluracil, a benzophenone derivative. Material and methods. The study investigated reproductive toxicity, embryotoxicity, immunotoxicity, genotoxic (in micronucleus test in and comet assay) and allergenic properties of the test itemcompound. It was tested on three species of animals in two doses: the estimated therapeutic dose (1 TD) and its tenfold equivalent (10 TD). Taking into account the metabolic coefficients, the doses for rats (Rattus) were 9 and 90 mg/kg, for mice (Mus musculus), 21 and 210 mg/kg, and for guinea pigs (Cavia porcellus), 8 and 80 mg/kg, respectively. Results and discussion. According to the obtained results, a favorable safety profile of the tested compound was established. Negative effects on the immune system, reproductive function, the body of pregnant animals and the fetus were not observed, as well as the compound did not have genotoxic and allergenic properties. Conclusion. These data allows to consider the studied compound as a promising therapeutic candidate for the treatment of HIV-1 infection.
D N Nosik - One of the best experts on this subject based on the ideXlab platform.
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antiviral activity of extracts of basidiomycetes and humic compounds substances against human immunodeficiency virus retroviridae Orthoretrovirinae lentivirus human immunodeficiency virus 1 and herpes simplex virus herpesviridae simplexvirus human al
Voprosy virusologii, 2020Co-Authors: D N Nosik, N N Nosik, T V Teplyakova, O A Lobach, I A Kiseleva, N G Kondrashina, M S Bochkova, G G AnankoAbstract:Introduction . One of the most urgent problem of modern medicine is the fight against the disease caused by the Human Immunodeficiency Virus (HIV) – HIV infection. The chemical compounds have improved the situation for infected people, but they are toxic, disrupt the metabolism and cannot eliminate the integrated virus from the body. The emergence of resistant HIV strains makes these treatments ineffective. Often, the death of HIV-infected people occurs as a result of the development of opportunistic infections caused by viruses of the Herpesviridae family. Therefore, the search for new therapeutic and preventive drugs that are less toxic and active against several viruses at the same time is relevant. Basidiomycetes , higher fungi, are a source of medicinal compounds that have antimicrobial properties, as well as antiviral ones. Humic compounds (HS) of various nature also have antiviral activity. The aim of the study was to obtain nontoxic compounds from the basidiomycete Inonotus obliquus and humic compounds from brown coals and to test their activity against viruses that are pathogenic to humans: HIV and Herpes Simplex Virus (HSV). Material and methods . The antiviral activity of melanin extracts obtained from the culture of the chaga fungus Inonotus obliquus and HS from the brown coal of the Kansko-Achinsk Deposit was studied using a model of MT-4 lymphoblastoid cells infected with HIV type 1 (HIV–1) strains and a monolayer culture of Vero cells infected with HSV type 1 (HSV-1) using virological and statistical research methods. Results and discussion . It was found that all the studied compounds did not have a cytotoxic effect on cells at a concentration of 100 mcg / ml. It was shown that extracts of basidiomycetes and HS have antiviral activity against HIV-1 and HSV-1. EC 50 (50%-effective concentration) for HIV-1 was 3.7–5.0 mcg/ml, selectivity index 28–35. Antiherpetic activity was detected at a dose of 50–100 mcg/ml. The antiviral effectiveness of melanin compounds was established both in the «preventive» (2 hours before cell infection) and in the «therapeutic» regimen of drug administration, both for HIV-1 and HSV-1. The presence of antiviral activity of melanin and HS in relation to the RNA-containing HIV-1 virus and DNA-containing HSV-1 virus in our study coincides with the results of a number of authors in relation to influenza viruses, herpes virus, HIV, hepatitis B virus, Coxsackievirus, smallpox vaccine virus, which suggests that the type of nucleic acid in the virus does not play a fundamental role in the antiviral action of these drugs. It is also clear that HS is effective against both enveloped and non-enveloped viruses. Conclusion . In general, it can be concluded that melanin and humic compounds are characterized by low toxicity in the presence of both virucidal and antiviral activity. This allows us to consider the studied compounds as the basis for creating safe medicines that are effective against pathogens of various viral infections.
