The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Li-an Wu - One of the best experts on this subject based on the ideXlab platform.
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development and characterization of a mouse floxed bmp2 Osteoblast Cell line that retains Osteoblast genotype and phenotype
Cell and Tissue Research, 2011Co-Authors: Lynn Wang, Yan Dong Mu, Junsheng Feng, Stephen E Harris, Andrew Baker, Li-an Wu, Kevin J. Donly, Mary MacdougallAbstract:Bone morphogenetic protein 2 (Bmp2) is essential for Osteoblast differentiation and osteogenesis. Generation of floxed Bmp2 Osteoblast Cell lines is a valuable tool for studying the effects of Bmp2 on Osteoblast differentiation and its signaling pathways during skeletal metabolism. Due to relatively limited sources of primary Osteoblasts, we have developed Cell lines that serve as good surrogate models for the study of Osteoblast Cell differentiation and bone mineralization. In this study, we established and characterized immortalized mouse floxed Bmp2 Osteoblast Cell lines. Primary mouse floxed Bmp2 Osteoblasts were transfected with pSV3-neo and clonally selected. These transfected Cells were verified by PCR and immunohistochemistry. To determine the genotype and phenotype of the immortalized Cells, Cell morphology, proliferation, differentiation and mineralization were analyzed. Also, expression of Osteoblast-related gene markers including Runx2, Osx, ATF4, Dlx3, bone sialoprotein, dentin matrix protein 1, osteonectin, osteocalcin and osteopontin were examined by quantitative RT-PCR and immunohistochemistry. These results showed that immortalized floxed Bmp2 Osteoblasts had a higher proliferation rate but preserved their genotypic and phenotypic characteristics similar to the primary Cells. Thus, we, for the first time, describe the development of immortalized mouse floxed Bmp2 Osteoblast Cell lines and present a useful model to study Osteoblast biology mediated by BMP2 and its downstream signaling transduction pathways.
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immortalization and characterization of mouse floxed bmp2 4 Osteoblasts
Biochemical and Biophysical Research Communications, 2009Co-Authors: Iris Ortizgonzalez, Wuchen Yang, Mary Macdougall, Li-an Wu, Guohua Yuan, Guobin Yang, Kevin J. DonlyAbstract:Generation of a floxed Bmp2/4 Osteoblast Cell line is a valuable tool for studying the modulatory effects of Bmp2 and Bmp4 on Osteoblast differentiation as well as relevant molecular events. In this study, primary floxed Bmp2/4 mouse Osteoblasts were cultured and transfected with simian virus 40 large T-antigen. Transfection was verified by polymerase chain reaction (PCR) and immunohistochemistry. To examine the characteristics of the transfected Cells, morphology, proliferation and mineralization were analyzed, expression of Cell-specific genes including Runx2, ATF4, Dlx3, Osx, dentin matrix protein 1, bone sialoprotein, osteopontin, osteocalcin, osteonectin and collagen type I was detected. These results show that transfected floxed Bmp2/4 Osteoblasts bypassed senescence with a higher proliferation rate, but retain the genotypic and phenotypic characteristics similar to the primary Cells. Thus, we for the first time demonstrate the establishment of an immortalized mouse floxed Bmp2/4 Osteoblast Cell line.
Pietro Favia - One of the best experts on this subject based on the ideXlab platform.
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improved Osteoblast Cell affinity on plasma modified 3 d extruded pcl scaffolds
Acta Biomaterialia, 2013Co-Authors: Marco Domingos, Francesca Intranuovo, Antonio Gloria, R Gristina, Luigi Ambrosio, Paulo Bartolo, Pietro FaviaAbstract:Cellular adhesion and proliferation inside three-dimensional synthetic scaffolds represent a major challenge in tissue engineering. Besides the surface chemistry of the polymers, it is well recognized that scaffold internal architecture, namely pore size/shape and interconnectivity, has a strong effect on the biological response of Cells. This study reports for the first time how polycaprolactone (PCL) scaffolds with controlled micro-architecture can be effectively produced via bioextrusion and used to enhance the penetration of plasma deposited species. Low-pressure nitrogen-based coatings were employed to augment Cell adhesion and proliferation without altering the mechanical properties of the structures. X-ray photoelectron spectroscopy carried out on different sections of the scaffolds indicates a uniform distribution of nitrogen-containing groups throughout the entire porous structure. In vitro biological assays confirm that plasma deposition sensitively promotes the activity of Saos-2 Osteoblast Cells, leading to a homogeneous colonization of the PCL scaffolds.
Byongtaek Lee - One of the best experts on this subject based on the ideXlab platform.
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functional nanofiber mat of polyvinyl alcohol gelatin containing nanoparticles of biphasic calcium phosphate for bone regeneration in rat calvaria defects
Journal of Biomedical Materials Research Part A, 2013Co-Authors: Nguyen Thuy Ba Linh, Kapho Lee, Byongtaek LeeAbstract:New biodegradable mats was successfully obtained by functional polyvinyl alcohol (PVA)/Gelatin (GE) blend fiber mats containing different BCP amounts (20, 40, and 50 w/v%) of bipha- sic calcium phosphate (BCP) nanoparticles for bone regeneration. BCP nanoparticles were loaded and dispersed successfully in the PVA/GE fibrous matrix. The addition of BCP was found to have increased fiber diameter, tensile strength, Osteoblast Cell adhesion, proliferation, and protein expression. Compared to the others, the 50% BCP-loaded electrospun PVA/GE fibers had the most favor- able mechanical properties, Cell attachment and growth, and pro- tein expression. In vivo bone formation was examined using rat models, and increased bone formation was observed for the 50% BCP-loaded electrospun PVA/GE blends within 2 and 4 weeks. This result suggests that the 50% BCP-PVA/GE composite nano- fiber mat has high potential for use in the field of bone regenera- tion and tissue engineering. V
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electrospinning of polyvinyl alcohol gelatin nanofiber composites and cross linking for bone tissue engineering application
Journal of Biomaterials Applications, 2012Co-Authors: Nguyen Thuy Ba Linh, Byongtaek LeeAbstract:A three-dimensional polymer composite system consisting of polyvinyl alcohol/gelatin (PVA/GE) was fabricated via the electrospinning method and physically cross linked by methanol treatment. The effects of cross-linking between PVA/GE blend on physical, mechanical, and biological properties were investigated. After treating with methanol, PVA/GE mats become dense, hard, and aggregative with increased resistance to water dissolution. Osteoblasts like MG-63 Cells were seeded on the surfaces of the cross linked PVA/GE mats and were found to attach firmly by expressing philopodial extensions. In addition, MTT assay and Western Blot analysis confirmed that the Cells readily proliferated on the cross linked PVA/GE scaffolds. The Osteoblast Cell-matrix interaction demonstrated that the active biocompatibility of the mats was facilitated by using GE and cross-linking. In conclusion, our results suggest that cross-linked PVA/GE scaffolds hold promise for tissue engineering applications, especially in the field of artificial bone implant.
Mary Macdougall - One of the best experts on this subject based on the ideXlab platform.
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development and characterization of a mouse floxed bmp2 Osteoblast Cell line that retains Osteoblast genotype and phenotype
Cell and Tissue Research, 2011Co-Authors: Lynn Wang, Yan Dong Mu, Junsheng Feng, Stephen E Harris, Andrew Baker, Li-an Wu, Kevin J. Donly, Mary MacdougallAbstract:Bone morphogenetic protein 2 (Bmp2) is essential for Osteoblast differentiation and osteogenesis. Generation of floxed Bmp2 Osteoblast Cell lines is a valuable tool for studying the effects of Bmp2 on Osteoblast differentiation and its signaling pathways during skeletal metabolism. Due to relatively limited sources of primary Osteoblasts, we have developed Cell lines that serve as good surrogate models for the study of Osteoblast Cell differentiation and bone mineralization. In this study, we established and characterized immortalized mouse floxed Bmp2 Osteoblast Cell lines. Primary mouse floxed Bmp2 Osteoblasts were transfected with pSV3-neo and clonally selected. These transfected Cells were verified by PCR and immunohistochemistry. To determine the genotype and phenotype of the immortalized Cells, Cell morphology, proliferation, differentiation and mineralization were analyzed. Also, expression of Osteoblast-related gene markers including Runx2, Osx, ATF4, Dlx3, bone sialoprotein, dentin matrix protein 1, osteonectin, osteocalcin and osteopontin were examined by quantitative RT-PCR and immunohistochemistry. These results showed that immortalized floxed Bmp2 Osteoblasts had a higher proliferation rate but preserved their genotypic and phenotypic characteristics similar to the primary Cells. Thus, we, for the first time, describe the development of immortalized mouse floxed Bmp2 Osteoblast Cell lines and present a useful model to study Osteoblast biology mediated by BMP2 and its downstream signaling transduction pathways.
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immortalization and characterization of mouse floxed bmp2 4 Osteoblasts
Biochemical and Biophysical Research Communications, 2009Co-Authors: Iris Ortizgonzalez, Wuchen Yang, Mary Macdougall, Li-an Wu, Guohua Yuan, Guobin Yang, Kevin J. DonlyAbstract:Generation of a floxed Bmp2/4 Osteoblast Cell line is a valuable tool for studying the modulatory effects of Bmp2 and Bmp4 on Osteoblast differentiation as well as relevant molecular events. In this study, primary floxed Bmp2/4 mouse Osteoblasts were cultured and transfected with simian virus 40 large T-antigen. Transfection was verified by polymerase chain reaction (PCR) and immunohistochemistry. To examine the characteristics of the transfected Cells, morphology, proliferation and mineralization were analyzed, expression of Cell-specific genes including Runx2, ATF4, Dlx3, Osx, dentin matrix protein 1, bone sialoprotein, osteopontin, osteocalcin, osteonectin and collagen type I was detected. These results show that transfected floxed Bmp2/4 Osteoblasts bypassed senescence with a higher proliferation rate, but retain the genotypic and phenotypic characteristics similar to the primary Cells. Thus, we for the first time demonstrate the establishment of an immortalized mouse floxed Bmp2/4 Osteoblast Cell line.
Muhammad Yar - One of the best experts on this subject based on the ideXlab platform.
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hydroxypropylmethyl Cellulose hpmc crosslinked chitosan ch based scaffolds containing bioactive glass bg and zinc oxide zno for alveolar bone repair
Carbohydrate Polymers, 2018Co-Authors: Rabia Zeeshan, Zeeshan Mutahir, Haffsah Iqbal, Moazzam Ali, Farasat Iqbal, Kashif Ijaz, Faiza Sharif, Asma Tufail Shah, Aqif Anwar Chaudhry, Muhammad YarAbstract:Abstract The success of a dental implant relies on the presence of an optimal alveolar ridge. The aim of this study was to fabricate HPMC crosslinked chitosan based scaffolds for alveolar bone repair. Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132–90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively. Cytotoxicity analysis at day 2, 4 and 8 demonstrated that all composites were non-toxic and supported Cellular viability. Calcein AM/propidium iodide staining, Hoechst nuclear staining and Cell adhesion assay reiterated that scaffolds supported pre-Osteoblast Cell growth, adhesion and proliferation. Differentiation potential of pre-Osteoblast Cells was enhanced as confirmed by alkaline phosphate assay. Furthermore, loss of S. aureus viability as low as 35% was attributed to synergistic effects of components. Overall, our results suggest that HPMC crosslinked scaffolds are potential candidates for alveolar bone repair.
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chitosan hydroxyapatite ha hydroxypropylmethyl Cellulose hpmc spongy scaffolds synthesis and evaluation as potential alveolar bone substitutes
Colloids and Surfaces B: Biointerfaces, 2017Co-Authors: Haffsah Iqbal, Rabia Zeeshan, Zeeshan Mutahir, Moazzam Ali, Farasat Iqbal, Aqif Anwar Chaudhry, Muhammad Yar, Muhammad Azhar Hayat Nawaz, Lubna Shahzadi, Shifang LuanAbstract:Alveolar bone loss is associated with infections and its augmentation is a pre-requisite for the success of dental implants. In present study, we aim to develop and evaluate novel freeze dried doxycycline loaded chitosan (CS)/hydroxyapatite (HA) spongy scaffolds where hydroxypropylmethyl Cellulose (HPMC) was added as a crosslinker. Scaffolds displayed compressive strength of 14MPa/cm3 and 0.34 as elastic response. The interconnected pore diameter was 41-273μm, favorably provided the template supporting Cells and transport. An overall 10% degradation was seen after 14day's studies at pH 7.4 in PBS. Doxycycline hyclate, a frequently used drug to counter oral infections, demonstrated an initial burst release (6-8h), followed by a sustain release profile for the remaining 64h. CS/HA/HPMC scaffolds were nontoxic and promoted pre-Osteoblast Cell viability as seen with live/dead calcein staining after 24h where scaffolds with 10% and 25% HPMC by weight of scaffold had more viable Cells. Scaffolds with 10%, 20% and 25% HPMC by weight of scaffold showed efficient Cellular adhesion as seen in scanning electron microscopy images (day 8) indicating that pre-Osteoblast Cells were able to adhere well on the surface and into the porous structure via cytoplasmic extensions. Hoechst 33258 nuclear staining at day 2 and 8 indicated Cell proliferation which was further supported byMTT assay at day 2, 4 and 8. Although all scaffolds supported pre-Osteoblast Cell viability, alkaline phosphatase (ALP) staining demonstrated that upon induction, differentiation was pronounced in case of scaffolds with 10% HMPC scaffolds. Conclusively, these materials having all the required mechanical and biological properties are potential candidates for alveolar bone regeneration.
