The Experts below are selected from a list of 756 Experts worldwide ranked by ideXlab platform
Shuai Chen - One of the best experts on this subject based on the ideXlab platform.
-
gambogic acid inhibits osteoclast formation and ovariectomy induced Osteoporosis by suppressing the jnk p38 and akt signalling pathways
Biochemical Journal, 2015Co-Authors: Shuai ChenAbstract:Excessive osteoclast formation and bone resorption are key causes of Osteoporosis. Natural compounds can serve as alternative therapeutic agents for the prevention and treatment of Osteoporosis, and some natural compounds may have advantages over traditional drugs. In the present paper, we report that the natural compound GBA (gambogic acid), which is bioavailable, effective and less toxic, inhibits osteoclast formation, thereby attenuating osteoclastic bone resorption in vitro . Further in vivo studies demonstrated that GBA prevented Ovariectomy-Induced bone loss in a dose-dependent manner. Moreover, we demonstrated that GBA suppressed RANKL (receptor activator of nuclear factor κB ligand)-induced JNK (c-Jun N-terminal kinase), p38 and Akt phosphorylation. Taken together, our results demonstrate that GBA inhibits osteoclast formation in vitro and in vivo , suggesting that it is of potential value in the treatment of osteoclast-related diseases. * BMM, : bone marrow macrophage; BV/TV, : trabecular bone volume per total volume; CCK-8, : cell counting kit-8; μCT, : micro-computed tomography; DC-STAMP, : dendritic cell-specific transmembrane protein; ERK, : extracellular-signal-regulated kinase; GAPDH, : glyceraldehyde-3-phosphate dehydrogenase; GBA, : gambogic acid; H&E, : haematoxylin and eosin; JNK, : c-Jun N-terminal kinase; MAPK, : mitogen-activated protein kinase; M-CSF, : macrophage colony-stimulating factor; α-MEM, : Eagle’s minimal essential medium with the Alpha modification; MKK, : mitogen-activated protein kinase kinase; MM, : multiple myeloma; NFATc1, : nuclear factor of activated T-cells c1; NF-κB, : nuclear factor κB; OcA/BA, : ratio of osteoclast area to bone area; OcN/BA, : number of TRAP-positive multinucleated osteoclasts normalized with bone area; OVX, : ovariectomy; RANKL, : receptor activator of nuclear factor κB ligand; TBST, : TBS with Tween 20; Tb.N, : trabecular number; Tb.Sp, : trabecular separation; Tb.Th, : trabecular thickness; TRAP, : tartrate-resistant acid phosphatase; V-ATPase, : vacuolar ATPase
Hanan A Saleh - One of the best experts on this subject based on the ideXlab platform.
-
Olive Oil effectively mitigates Ovariectomy-Induced Osteoporosis in rats
BMC Complementary and Alternative Medicine, 2011Co-Authors: Nermine K Saleh, Hanan A SalehAbstract:Background Osteoporosis, a reduction in bone mineral density, represents the most common metabolic bone disease. Postmenopausal women are particularly susceptible to Osteoporosis when their production of estrogen declines. For these women, fracture is a leading cause of morbidity and mortality. This study was conducted to evaluate the protective effects of olive oil supplementation against Osteoporosis in ovariectomized (OVX) rats. Methods We studied adult female Wistar rats aged 12-14 months, divided into three groups: sham-operated control (SHAM), ovariectomized (OVX), and ovariectomized rats supplemented with extravirgin olive oil (Olive-OVX) orally for 12 weeks; 4 weeks before ovariectomy and 8 weeks after. At the end of the experiment, blood samples were collected. Plasma levels of calcium, phosphorus, alkaline phosphatase (ALP), malondialdehyde (MDA), and nitrates were assayed. Specimens from both the tibia and the liver were processed for light microscopic examination. Histomorphometric analysis of the tibia was also performed. Results The OVX-rats showed a significant decrease in plasma calcium levels, and a significant increase in plasma ALP, MDA, and nitrates levels. These changes were attenuated by olive oil supplementation in the Olive-OVX rats. Light microscopic examination of the tibia of the OVX rats revealed a significant decrease in the cortical bone thickness (CBT) and the trabecular bone thickness (TBT). In addition, there was a significant increase in the osteoclast number denoting bone resorption. In the Olive-OVX rats these parameters were markedly improved as compared to the OVX group. Examination of the liver specimens revealed mononuclear cellular infiltration in the portal areas in the OVX-rats which was not detected in the Olive-OVX rats. Conclusions Olive oil effectively mitigated Ovariectomy-Induced Osteoporosis in rats, and is a promising candidate for the treatment of postmenopausal Osteoporosis.
-
Olive Oil effectively mitigates Ovariectomy-Induced Osteoporosis in rats
BMC Complementary and Alternative Medicine, 2011Co-Authors: Nermine K Saleh, Hanan A SalehAbstract:Osteoporosis, a reduction in bone mineral density, represents the most common metabolic bone disease. Postmenopausal women are particularly susceptible to Osteoporosis when their production of estrogen declines. For these women, fracture is a leading cause of morbidity and mortality. This study was conducted to evaluate the protective effects of olive oil supplementation against Osteoporosis in ovariectomized (OVX) rats. We studied adult female Wistar rats aged 12-14 months, divided into three groups: sham-operated control (SHAM), ovariectomized (OVX), and ovariectomized rats supplemented with extravirgin olive oil (Olive-OVX) orally for 12 weeks; 4 weeks before ovariectomy and 8 weeks after. At the end of the experiment, blood samples were collected. Plasma levels of calcium, phosphorus, alkaline phosphatase (ALP), malondialdehyde (MDA), and nitrates were assayed. Specimens from both the tibia and the liver were processed for light microscopic examination. Histomorphometric analysis of the tibia was also performed. The OVX-rats showed a significant decrease in plasma calcium levels, and a significant increase in plasma ALP, MDA, and nitrates levels. These changes were attenuated by olive oil supplementation in the Olive-OVX rats. Light microscopic examination of the tibia of the OVX rats revealed a significant decrease in the cortical bone thickness (CBT) and the trabecular bone thickness (TBT). In addition, there was a significant increase in the osteoclast number denoting bone resorption. In the Olive-OVX rats these parameters were markedly improved as compared to the OVX group. Examination of the liver specimens revealed mononuclear cellular infiltration in the portal areas in the OVX-rats which was not detected in the Olive-OVX rats. Olive oil effectively mitigated Ovariectomy-Induced Osteoporosis in rats, and is a promising candidate for the treatment of postmenopausal Osteoporosis.
Mohammad Bayat - One of the best experts on this subject based on the ideXlab platform.
-
Improvement in viability and mineralization of osteoporotic bone marrow mesenchymal stem cell through combined application of photobiomodulation therapy and oxytocin
Lasers in Medical Science, 2020Co-Authors: Somaye Fallahnezhad, Seyed Kamran Ghoreishi, Sufan Chien, Vahid Jajarmi, Sarira Shahnavaz, Abdullah Amini, Mahsa Kazemi, Mohammad BayatAbstract:The probable positive effects of photobiomodulation therapy (PBMT) and oxytocin (OT) treatments together or alone were evaluated on cell viability along with the changes in the gene expression of Osteocalcin (OC), Osteoprotegerin (OPG), and Runt-related transcription factor 2 (Runx2) levels of sham (healthy)-Bone marrow mesenchymal stem cell(BMMSC) and Ovariectomy-Induced Osteoporosis (OVX)-BMMSC. BMMSC was harvested from healthy and OVX rats and was cultured in osteogenic induction medium (OIM). There were five groups of BMMSCs: (1) sham -BMMSCs; (2) control -OVX-BMMSCs; (3) OT-treated-OVX-BMMSCs; (4) PBMT–treated-OVX-BMMSCs, and (5) OT + PBMT-OVX-BMMSCs. In all 5 groups, BMMSC viability and proliferation as well as gene expression of OC, OPG, and RUNX2 were evaluated. PBMT and PBMT + OT treatments showed a promising effect on the increased viability of OVX-BMMSC (ANOVA test; LSD test, p = 0.01, p = 0.002). The results of gene expression analysis revealed that the sham- BMMSCs responded optimally to OT treatment. It was also found that OVX-BMMSCs responded optimally to PBMT + OT and PBMT treatments at early and middle stages of osteogenic induction process. Nevertheless, they responded optimally to PBMT + OT and OT especially at the late stage of osteogenic induction process. PBMT and PBMT + OT treatments significantly increased viability of OVX-BMMSC in OIM in vitro. Both PBMT and PBMT + OT treatments could promote mineralization of OVX-BMMSC in the culture medium at early and middle stages of osteogenic induction process. Both OT and PBMT + OT treatments could promote mineralization of OVX-BMMSC in vitro at late stages of osteogenic induction process.
-
Effects of pentoxifylline and alendronate on fracture healing in Ovariectomy-Induced Osteoporosis in rats
Veterinary research forum : an international quarterly journal, 2019Co-Authors: Mohammad Farahani, Reza Masteri Farahani, Mohammad-amin Abdollahifar, Ghatrehsamani, Seyed Kamran Ghoreishi, Behnam Hajihossainlou, Sufan Chien, Atarodsadat Mostafavinia, Fatemehalsadat Rezaei, Mohammad BayatAbstract:Osteoporosis is determined by decreased bone strength that increases the threat of fractures. The aim of this study was to evaluate the effects of pentoxifylline (PTX) and alendronate (ALN), on the stereological parameters, and gene expression in callus of fracture in an experimental rat model of Ovariectomy-Induced Osteoporosis (OVX). The OVX was induced in 90 female rats. Fourteen weeks later, a complete fracture on the right femur was made. Rats were divided into five groups: 1) control: no treatment; 2) sham: received daily distilled water; 3) daily 3.00 mg kg(-1) ALN subcutaneously (SC); 4) daily 200 mg kg(-1) PTX (SC) and 5) daily PTX (SC) + ALN (same doses). The osteoclast count was significantly lower in all treatment groups, at 21 and 56 days post-surgery, compared to the control and sham groups. The PTX significantly increased total callus volume at 21 and 56 days post-surgery, compared to the other groups. The PTX+ALN treatment significantly increased both cortical bone volume on day 21, and osteocyte and osteoblast numbers on day 56, compared to the control and sham groups. It can be concluded that PTX and ALN have antiresorptive effects, in OVX rats. Also, PTX has increased the extracellular matrix on both 21 and 56 days after surgery, compared to the other groups. PTX+ALN elevated cortical bone volume on day 21, and osteocyte and osteoblast numbers compared to the control and sham groups on day 56. (C) 2019 Urmia University. All rights reserved.
-
Effect of in vivo low-level laser therapy on bone marrow-derived mesenchymal stem cells in Ovariectomy-Induced Osteoporosis of rats
Journal of Photochemistry and Photobiology B-biology, 2017Co-Authors: Ataroalsadat Mostafavinia, Seyed Kamran Ghoreishi, Behnam Hajihossainlou, Leila Dehdehi, Mohammad BayatAbstract:Abstract Postmenopausal Osteoporosis (PMOP) is considered by decreased bone strength that escalates the threat of fractures. Positive effects of photobiomodulation (PBM) with pulse wave have been demonstrated in cell culture and animal models. The aim of this study was to assess the in vivo effects of PBM on viability and calcium ion release of ovariectomy induced Osteoporosis (OVX) - bone marrow derived mesenchymal stem cells (BMMSCs). Material and Methods 18 female rats were distributed into the following groups: 1) control healthy, 2) LASER-healthy (890 nm, 80 Hz, 1.5 J/cm2, three days weekly, 60 days), 3) control OVX, 4) LASER-OVX, 5) Alendronate (Alen.)-OVX [0.5 mg/kg, 5 days per week, 60 days], and 6) Alen. + LASER-OVX. Ovariectomy was done on rats of groups 3, 4, 5 and 6. After that all rats were euthanized and their MSC harvested and cultured in complete osteogenic medium. In all groups, BMMSC viability, and calcium colorimetric assay were performed. Results We observed a significant increase in optical density (OD) of BMMSCs viability in LASER healthy group compared to control-OVX, Alen.-OVX, LASER-OVX, LASER + Alen.-OVX, groups. LASER + Alen.-OVX group displayed a significant escalation in OD of BMMSCs viability compared to LASER-OVX, Alen.-OVX, and control-OVX groups. There were a significant increase in calcium ion release of LASER-healthy group compared to control healthy, control-OVX, Alen.-OVX, LASER-OVX, and LASER + Alen.-OVX groups. LASER + Alen.-OVX group displayed a significant escalation in calcium ion release compared to LASER-OVX, Alen.-OVX, and control-OVX groups. Conclusion Pulse wave (PW) PBM significantly stimulated viability and cell proliferation of healthy BMMSCs compared to those of control-OVX, OVX-alendronate, OVX-LASER, and LASER + alendronate-OVX. In addition stimulatory effect of LASER + alendronate on viability and cell proliferation of OVX-BMMSCs compared to those of control-OVX, alendronate-OVX, and LASER-OVX groups were found.
-
The effects of pentoxifylline adminstration on fracture healing in a postmenopausal osteoporotic rat model
Laboratory Animal Research, 2017Co-Authors: Mohammad Mahdi Vashghani Farahani, Mohammad-amin Abdollahifar, Reza Ahadi, Mohammad BayatAbstract:Previous studies report positive effects of pentoxifylline (PTX) alone or in combination with other drugs on some pathologic bone diseases as well as an ability to accelerate osteogensis and fracture healing in both animal models and human patients. The aim of this present study was to evaluate the effects of PTX administration on Hounsfield unit and bone strength at catabolic response (bone resorbing) of a fracture in an experimental rat model of ovariectomy induced Osteoporosis (OVX-D). Thirty adult female rats were divided into groups as follows: 1 (OVX, control, no treatment); 2 (OVX, sham: daily distilled water); 3 (OVX, daily alendronate: 3 mg/kg); 4 (OVX, twice daily 100 mg/kg PTX) and 5 (OVX, PTX+alenderonate). OVX was induced by bilateral ovariectomy in all rats. A complete standardized osteotomy of the right femur was made after 3.5 months. PTX and alendronate treatments were performed for eight weeks. Then, rats were euthanized and had its right femur subjected to computerized tomography scanning for measuring Hounsfield unit; eventually, the samples were sent for a three point bending test for evaluation of the bone strength. Administration of PTX with 200 mg/kg and alendronate alone and in combination showed no significant alteration in Hounsfield unit and biomechanical properties of repairing callus of the complete osteotomy compared with the control group. Results showed increased bending stiffness and stress high load mean values of repairing complete osteotomy in PTX-treated rats compared to the control OVX-D.
Nermine K Saleh - One of the best experts on this subject based on the ideXlab platform.
-
Olive Oil effectively mitigates Ovariectomy-Induced Osteoporosis in rats
BMC Complementary and Alternative Medicine, 2011Co-Authors: Nermine K Saleh, Hanan A SalehAbstract:Background Osteoporosis, a reduction in bone mineral density, represents the most common metabolic bone disease. Postmenopausal women are particularly susceptible to Osteoporosis when their production of estrogen declines. For these women, fracture is a leading cause of morbidity and mortality. This study was conducted to evaluate the protective effects of olive oil supplementation against Osteoporosis in ovariectomized (OVX) rats. Methods We studied adult female Wistar rats aged 12-14 months, divided into three groups: sham-operated control (SHAM), ovariectomized (OVX), and ovariectomized rats supplemented with extravirgin olive oil (Olive-OVX) orally for 12 weeks; 4 weeks before ovariectomy and 8 weeks after. At the end of the experiment, blood samples were collected. Plasma levels of calcium, phosphorus, alkaline phosphatase (ALP), malondialdehyde (MDA), and nitrates were assayed. Specimens from both the tibia and the liver were processed for light microscopic examination. Histomorphometric analysis of the tibia was also performed. Results The OVX-rats showed a significant decrease in plasma calcium levels, and a significant increase in plasma ALP, MDA, and nitrates levels. These changes were attenuated by olive oil supplementation in the Olive-OVX rats. Light microscopic examination of the tibia of the OVX rats revealed a significant decrease in the cortical bone thickness (CBT) and the trabecular bone thickness (TBT). In addition, there was a significant increase in the osteoclast number denoting bone resorption. In the Olive-OVX rats these parameters were markedly improved as compared to the OVX group. Examination of the liver specimens revealed mononuclear cellular infiltration in the portal areas in the OVX-rats which was not detected in the Olive-OVX rats. Conclusions Olive oil effectively mitigated Ovariectomy-Induced Osteoporosis in rats, and is a promising candidate for the treatment of postmenopausal Osteoporosis.
-
Olive Oil effectively mitigates Ovariectomy-Induced Osteoporosis in rats
BMC Complementary and Alternative Medicine, 2011Co-Authors: Nermine K Saleh, Hanan A SalehAbstract:Osteoporosis, a reduction in bone mineral density, represents the most common metabolic bone disease. Postmenopausal women are particularly susceptible to Osteoporosis when their production of estrogen declines. For these women, fracture is a leading cause of morbidity and mortality. This study was conducted to evaluate the protective effects of olive oil supplementation against Osteoporosis in ovariectomized (OVX) rats. We studied adult female Wistar rats aged 12-14 months, divided into three groups: sham-operated control (SHAM), ovariectomized (OVX), and ovariectomized rats supplemented with extravirgin olive oil (Olive-OVX) orally for 12 weeks; 4 weeks before ovariectomy and 8 weeks after. At the end of the experiment, blood samples were collected. Plasma levels of calcium, phosphorus, alkaline phosphatase (ALP), malondialdehyde (MDA), and nitrates were assayed. Specimens from both the tibia and the liver were processed for light microscopic examination. Histomorphometric analysis of the tibia was also performed. The OVX-rats showed a significant decrease in plasma calcium levels, and a significant increase in plasma ALP, MDA, and nitrates levels. These changes were attenuated by olive oil supplementation in the Olive-OVX rats. Light microscopic examination of the tibia of the OVX rats revealed a significant decrease in the cortical bone thickness (CBT) and the trabecular bone thickness (TBT). In addition, there was a significant increase in the osteoclast number denoting bone resorption. In the Olive-OVX rats these parameters were markedly improved as compared to the OVX group. Examination of the liver specimens revealed mononuclear cellular infiltration in the portal areas in the OVX-rats which was not detected in the Olive-OVX rats. Olive oil effectively mitigated Ovariectomy-Induced Osteoporosis in rats, and is a promising candidate for the treatment of postmenopausal Osteoporosis.
Sanjiv Neupane - One of the best experts on this subject based on the ideXlab platform.
-
Bortezomib prevents Ovariectomy-Induced Osteoporosis in mice by inhibiting osteoclast differentiation
Journal of Bone and Mineral Metabolism, 2018Co-Authors: Sanjiv NeupaneAbstract:Bone homeostasis is achieved through coordinated activities of bone-forming osteoblasts and bone-resorbing osteoclasts. When the balance is skewed in favor of osteoclasts due to hormonal or inflammatory issues, pathologic bone loss occurs leading to conditions such as Osteoporosis, rheumatoid arthritis, and periodontitis. Bortezomib is the first in-class of proteasome inhibitors used as an anti-myeloma agent. In the present study, we show that bortezomib directly inhibited the receptor activator of nuclear factor κB ligand (RANKL)—dependent osteoclast differentiation of mouse bone marrow macrophages. Bortezomib significantly reduced the induction of osteoclast marker genes and proteins including nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1). The intraperitoneal injection of bortezomib reduced Ovariectomy-Induced osteoclastogenesis and protected the mice from bone loss. These data propose novel use of bortezomib as a potential anti-resorptive agent.