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Yoosik Yoon - One of the best experts on this subject based on the ideXlab platform.
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Paeonia lactiflora extract suppresses cisplatin induced muscle wasting via downregulation of muscle specific ubiquitin e3 ligases nf κb signaling and cytokine levels
Journal of Ethnopharmacology, 2020Co-Authors: Jaewoong Jang, Hyunji Lee, Jaewon Song, Taehyun Bae, Minwoo Park, Young Kwon, Doheon Lee, Yoosik YoonAbstract:Abstract Ethnopharmacological relevance The dried root of Paeonia lactiflora Pall. (Radix Paeoniae) has been traditionally used to treat various inflammatory diseases in many Asian countries. Aim of the study Cisplatin is a broad-spectrum anticancer drug used in diverse types of cancer. However, muscle wasting is a common side effect of cisplatin chemotherapy. This study aimed to elucidate the effects of an ethanol extract of the root of Paeonia lactiflora Pall. (Radix Paeoniae, RP) on cisplatin-induced muscle wasting along with its molecular mechanism. Material and methods C57BL/6 mice were intraperitoneally injected with cisplatin and orally treated with RP. Megestrol acetate was used as a comparator drug. Skeletal muscle mass was measured as the weight of gastrocnemius and quadriceps muscles, and skeletal muscle function was measured by treadmill running time and grip strength. Skeletal muscle tissues were analyzed by RNAseq, western blotting, ELISA, and immunofluorescence microscopy. Results In mice treated with cisplatin, skeletal muscle mass and skeletal muscle function were significantly reduced. However, oral administration of RP significantly restored skeletal muscle mass and function in the cisplatin-treated mice. In the skeletal muscle tissues of the cisplatin-treated mice, RP downregulated NF-κB signaling and cytokine levels. RP also downregulated muscle-specific ubiquitin E3 ligases, resulting in the restoration of myosin heavy chain (MyHC) and myoblast determination protein (MyoD), which play crucial roles in muscle contraction and muscle differentiation, respectively. Conclusion RP restored skeletal muscle function and mass in cisplatin-treated mice by restoring the muscle levels of MyHC and MyoD proteins via downregulation of muscle-specific ubiquitin E3 ligases as well as muscle NF-κB signaling and cytokine levels.
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Paeonia lactiflora root extract suppresses cancer cachexia by down regulating muscular nf κb signalling and muscle specific e3 ubiquitin ligases in cancer bearing mice
Journal of Ethnopharmacology, 2020Co-Authors: Jaewoong Jang, Jaewon Song, Minwoo Park, Seyeon Chae, Seokmin Yoon, Yoosik YoonAbstract:Abstract Ethnopharmacological relevance The dried root of Paeonia lactiflora Pall. (Radix Paeoniae) has been traditionally used to treat various inflammatory diseases in many Asian countries. Aim of the study Cancer cachexia is a catabolic syndrome driven by inflammation and characterised by a loss of skeletal muscle. This study aimed to assess the effects of an ethanolic extract of Radix Paeoniae (RP) on cancer cachexia and elucidate its mechanism of action. Material and methods The anti-cachexic effect and mechanism of RP were examined in mouse models of cancer cachexia established in C57BL/6 mice by subcutaneously injecting Lewis lung carcinoma or MC38 colon carcinoma cells. Skeletal muscle tissues were analysed by RNAseq, real-time quantitative reverse transcription PCR, western blotting, and immunofluorescence microscopy. Megestrol acetate, which is recommended for the treatment of cachexia in cancer patients, was used as the comparator treatment in this study. Results In lung and colon cancer-bearing mice, RP significantly restored food intake and muscle mass, along with muscle function measured by grip strength and treadmill running time. In the skeletal muscle tissue of the cancer-bearing mice, RP suppressed NF-κB signalling and reduced inflammatory cytokines, including TNF-α, IL-6, and IL-1β; it also down-regulated the muscle-specific E3 ubiquitin ligases MuRF1 and MAFbx. Conclusion RP restored skeletal muscle function and mass in cancer-bearing mice by down-regulating the muscular NF-κB signalling pathway and muscle-specific E3 ubiquitin ligases. Our study indicates that RP is a potential candidate for development as a therapeutic agent against cancer cachexia.
Qianru Feng - One of the best experts on this subject based on the ideXlab platform.
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a simple thin layer chromatographic fingerprint method for distinguishing between radix Paeoniae rubra and radix Paeoniae alba
Journal of Liquid Chromatography & Related Technologies, 2009Co-Authors: Liu Yang, Shunjun Xu, Qianru Feng, Runtao TianAbstract:Abstract According to different processing methods and growth conditions, the dry roots of Paeonia lactiflora Pallas are generally classified as Radix Paeoniae Alba (Bai-shao) and Radix Paeoniae Rubra (Chi-shao), which have been officially recorded under different monographs in all editions of Chinese Pharmacopoeia. Although deriving from the same plant origin, the two herbs have individual chemical characteristics, which eventually result in their different efficacies. To better understand their clinical effects, it is important to display the similarity and differentiation of entire chemical patterns of the two drugs in detail. In the present study, the high performance thin layer chromatographic fingerprints combining digital scanning profiling were developed using two suites of mobile phases and derivatization reagents for the ‘high polarity component’ and ‘lipophilic component’ fractions contained in the herbal drugs, respectively. The unique properties of the HPTLC fingerprints were validated by ana...
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species differentiation and quality assessment of radix Paeoniae rubra chi shao by means of high performance liquid chromatographic fingerprint
Journal of Chromatography A, 2009Co-Authors: Shunjun Xu, Runtao Tian, Zhengtao Wang, Liu Yang, Qianru FengAbstract:There are two species under the monograph of Radix Paeoniae Rubrae (“Chi-shao” in Chinese) in Chinese Pharmacopoeia 2005 edition-Paeonia lactiflora Pallas and Paeonia veitchii Lynch. Due to different species and growing conditions, there are significant chemical differences between the two species, which may result in the improper clinical usage under the same name. Chemical pattern expressed by high performance liquid chromatographic (HPLC) fingerprint analysis can play an important role in species differentiation and quality control of Radix Paeoniae Rubra. In the present work, HPLC fingerprints of two kinds of Radix Paeoniae Rubra have been established and analysed with chemometric methods including similarity evaluation and principal component analysis. Both of the fingerprint common patterns of the two species comprise 13 characteristic peaks, nine of which were common peaks of the two species. However, significant differences between the roots of P. veitchii and P. lactiflora exist not only in the content of certain constituents, especially phenolic acids but also in peak-to-peak ratios expressed by the fingerprint patterns. According to the recent pharmacological studies on polyphenolic constituents, root originating from P. veitchii may possess better efficacy and quality than that from P. lactiflora. Our research reveals that further pharmacological investigation is very necessary to determine whether the two species should be embodied under the same monograph in Chinese Pharmacopoeia.
Jaewoong Jang - One of the best experts on this subject based on the ideXlab platform.
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Paeonia lactiflora extract suppresses cisplatin induced muscle wasting via downregulation of muscle specific ubiquitin e3 ligases nf κb signaling and cytokine levels
Journal of Ethnopharmacology, 2020Co-Authors: Jaewoong Jang, Hyunji Lee, Jaewon Song, Taehyun Bae, Minwoo Park, Young Kwon, Doheon Lee, Yoosik YoonAbstract:Abstract Ethnopharmacological relevance The dried root of Paeonia lactiflora Pall. (Radix Paeoniae) has been traditionally used to treat various inflammatory diseases in many Asian countries. Aim of the study Cisplatin is a broad-spectrum anticancer drug used in diverse types of cancer. However, muscle wasting is a common side effect of cisplatin chemotherapy. This study aimed to elucidate the effects of an ethanol extract of the root of Paeonia lactiflora Pall. (Radix Paeoniae, RP) on cisplatin-induced muscle wasting along with its molecular mechanism. Material and methods C57BL/6 mice were intraperitoneally injected with cisplatin and orally treated with RP. Megestrol acetate was used as a comparator drug. Skeletal muscle mass was measured as the weight of gastrocnemius and quadriceps muscles, and skeletal muscle function was measured by treadmill running time and grip strength. Skeletal muscle tissues were analyzed by RNAseq, western blotting, ELISA, and immunofluorescence microscopy. Results In mice treated with cisplatin, skeletal muscle mass and skeletal muscle function were significantly reduced. However, oral administration of RP significantly restored skeletal muscle mass and function in the cisplatin-treated mice. In the skeletal muscle tissues of the cisplatin-treated mice, RP downregulated NF-κB signaling and cytokine levels. RP also downregulated muscle-specific ubiquitin E3 ligases, resulting in the restoration of myosin heavy chain (MyHC) and myoblast determination protein (MyoD), which play crucial roles in muscle contraction and muscle differentiation, respectively. Conclusion RP restored skeletal muscle function and mass in cisplatin-treated mice by restoring the muscle levels of MyHC and MyoD proteins via downregulation of muscle-specific ubiquitin E3 ligases as well as muscle NF-κB signaling and cytokine levels.
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Paeonia lactiflora root extract suppresses cancer cachexia by down regulating muscular nf κb signalling and muscle specific e3 ubiquitin ligases in cancer bearing mice
Journal of Ethnopharmacology, 2020Co-Authors: Jaewoong Jang, Jaewon Song, Minwoo Park, Seyeon Chae, Seokmin Yoon, Yoosik YoonAbstract:Abstract Ethnopharmacological relevance The dried root of Paeonia lactiflora Pall. (Radix Paeoniae) has been traditionally used to treat various inflammatory diseases in many Asian countries. Aim of the study Cancer cachexia is a catabolic syndrome driven by inflammation and characterised by a loss of skeletal muscle. This study aimed to assess the effects of an ethanolic extract of Radix Paeoniae (RP) on cancer cachexia and elucidate its mechanism of action. Material and methods The anti-cachexic effect and mechanism of RP were examined in mouse models of cancer cachexia established in C57BL/6 mice by subcutaneously injecting Lewis lung carcinoma or MC38 colon carcinoma cells. Skeletal muscle tissues were analysed by RNAseq, real-time quantitative reverse transcription PCR, western blotting, and immunofluorescence microscopy. Megestrol acetate, which is recommended for the treatment of cachexia in cancer patients, was used as the comparator treatment in this study. Results In lung and colon cancer-bearing mice, RP significantly restored food intake and muscle mass, along with muscle function measured by grip strength and treadmill running time. In the skeletal muscle tissue of the cancer-bearing mice, RP suppressed NF-κB signalling and reduced inflammatory cytokines, including TNF-α, IL-6, and IL-1β; it also down-regulated the muscle-specific E3 ubiquitin ligases MuRF1 and MAFbx. Conclusion RP restored skeletal muscle function and mass in cancer-bearing mice by down-regulating the muscular NF-κB signalling pathway and muscle-specific E3 ubiquitin ligases. Our study indicates that RP is a potential candidate for development as a therapeutic agent against cancer cachexia.
Seyed Fazel Nabavi - One of the best experts on this subject based on the ideXlab platform.
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Neuroprotective effects of paeoniflorin in neurodegenerative diseases of the central nervous system
Phytochemistry Reviews, 2017Co-Authors: Azadeh Manayi, Sahar Omidpanah, Silvana Ficarra, Seyed Fazel Nabavi, Davide Barreca, Maria DagliaAbstract:Paeoniflorin is a monoterpene glycoside, the β-glucoside of paeoniflorigenin. It is one of the major components of Radix Paeoniae, the herbal product obtained from Paeonia lactiflora Pall., a medicinal plant used in folk medicine, especially in Chinese Traditional Medicine, to treat several human ailments, including dementia and cognitive decline. The neuroprotective effects of paeoniflorin have been studied by many investigations showing that its beneficial effects may derive from its anti-inflammatory and anti-apoptotic properties, and its ability to promote neuronal survival. This review evaluates scientific evidence on the neuroprotective properties of paeoniflorin. On the basis of literature data, paeoniflorin seems to be a promising compound for the prevention and treatment of neurodegenerative disorders, though definitive recommendation requires further studies.
Peigen Xiao - One of the best experts on this subject based on the ideXlab platform.
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chemical taxonomy of tree peony species from china based on root cortex metabolic fingerprinting
Phytochemistry, 2014Co-Authors: Chunnian He, Bing Peng, Yong Peng, Peigen XiaoAbstract:Abstract The section Moutan of the genus Paeonia consists of eight species that are confined to a small area in China. A wide range of metabolites, including monoterpenoid glucosides, flavonoids, tannins, stilbenes, triterpenoids, steroids, paeonols, and phenols, have been found in the species belonging to section Moutan. However, although previous studies have analyzed the metabolites found in these species, the metabolic similarities that can be used for the chemotaxonomic distinction of section Moutan species are not yet clear. In this study, HPLC–DAD-based metabolic fingerprinting was applied to the classification of eight species: Paeonia suffruticosa, Paeonia qiui, Paeonia ostii, Paeonia rockii, Paeonia jishanensis, Paeonia decomposita, Paeonia delavayi, and Paeonia ludlowii. In total, of the 47 peaks that exhibited an occurrence frequency of 75% in all 23 tree peony samples, 43 of these metabolites were identified according to their retention times and UV absorption spectra, together with combined HPLC–QTOF-MS. These data were compared with reference standard compounds. The 43 isolated compounds included 17 monoterpenoid glucosides, 11 galloyl glucoses, 5 flavonoids, 6 paeonols and 4 phenols. Principal component analysis (PCA), and hierarchical cluster analysis (HCA), showed a clear separation between the species based on metabolomics similarities and four groups were identified. The results exhibited good agreement with the classical classification based on the morphological characteristics and geographical distributions of the subsections Vaginatae F.C. Stern and Delavayanae F.C. Stern with the exception of P. decomposita, which was found to be a transition species between these two subsections. According to their metabolic fingerprinting characteristics, P. ostii and P. suffruticosa can be considered one species, and this result is consistent with the viewpoint of medicinal plant scientists but different from that of classical morphological processing. Significantly large variations were obtained in the metabolic profiles of P. delavayi, whereas no significant difference was found between P. delavayi and P. ludlowii. This indicates that these two species have a close genetic relationship. In conclusion, the combination of HPLC–DAD and multivariate analyses has great potential for guiding future chemotaxonomic studies to examine the potential pharmaceutical value of the effective constituents of tree peony species and appears to be able to clarify the confusion and skepticism associated with the reported morphology- and molecular phylogenetics-based taxonomy of tree peonies.