The Experts below are selected from a list of 165 Experts worldwide ranked by ideXlab platform
Li Yijing - One of the best experts on this subject based on the ideXlab platform.
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Prokaryotic expression of VP3 gene of infectious Pancreas Necrosis virus and antigenicity of expressed product.
JOURNAL OF FISHERIES OF CHINA, 2010Co-Authors: Zhao Lili, Liu Min, Ge Junwei, Qiao Xinyuan, Liu Weiwei, Zhao Yongxin, Ha Zhuo, Li YijingAbstract:Infectious pancreatic Necrosis(IPN) virus,the etiologic agent of infectious pancreatic Necrosis in salmonid fish,causes significant losses to the aquaculture industry.The gene for the viral inner capsid protein(VP3) was amplified by RT-PCR method from IPNV,and cloned into pET30b vector.The expression of recombinant plasmid pET30b-VP3 in E.coli BL21(DE3) was induced and detected by SDS-PAGE analysis.The predicted molecular weight for unmodified r-trunc VP3 was approximately 30 ku and this was found to be the case for E.coli protein.The amount of expression made up 30 percent of the bacteria protein total expression by thin layer scanning analysis.The results showed that the VP3 gene of IPNV can express successfully in E.coli BL21.The fusion protein was purified with ProBondTM resin from the suspension centrifuged and the antisera against VP3 protein was produced.The pET30b-VP3 fusion protein can be recognized by the positive serum of IPNV by Western-blotting analysis.The prepared antisera reacted specifically with IPNV antigen by indirect ELISA.The antisera against VP3 protein had OD values at least twice that obtained for the negative control serum at a dilution of 1 :25 600.The results showed that the expressed VP3 protein was immunogenical and antigenical which is the same as the natural IPNV VP3 protein.In this experiment the IPNV VP3 protein was expressed successfully by using prokaryotic expression system.The expressed fusion protein was active and the antisera against VP3 protein were produced.
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Surface expression of VP3 protein gene of infectious Pancreas Necrosis virus in Lactobacillus casei.
Chinese Journal of Preventive Veterinary Medicine, 2010Co-Authors: Liu Min, Zhao Lili, Ge Junwei, Qiao Xinyuan, Liu Weiwei, Zhao Yongxin, Li YijingAbstract:In this study,Lactobacillus casei 393 was used as a bacterial carrier for the expression of infectious Pancreas Necrosis virus (IPNV) protective antigen VP3 protein.The gene encoding IPNV VP3 protein was cloned into the Lactobacillus casei surface expression vector pPG1,and electro-transformed into Lactobacillus casei 393 to generate the recombinant system pPG1-VP3/Lactobacillus casei 393.The recombinant strain was induced by 1 % Lactose and the expressed protein about 31 ku was detected by SDS-PAGE and western blot.The indirect immunofluorescent test showed that the expressed protein was secreted on the cell surface Lactobacillus casei.This work provided the base for further immunological study of the IPNV VP3 protein.
Marcia I. Canto - One of the best experts on this subject based on the ideXlab platform.
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Sa1382 Differences in Outcomes Among Patients With Post-ERCP Pancreatitis and Alcohol-Induced Acute Pancreatitis
Gastroenterology, 2013Co-Authors: Mehak Idrees, Ayesha Kamal, Izzah Vasim, Eun Ji Shin, Venkata S. Akshintala, Ali Kord Valeshabad, Payal Saxena, Vikesh K. Singh, Anne Marie Lennon, Marcia I. CantoAbstract:G A A b st ra ct s during the first 4 days or .120 mg/L during the first week. Six patients had severe complications, three patients had Pancreas Necrosis, two had pseudocysts and one developed renal failure. One of those patients was admitted to intensive care unit. No patient died of acute pancreatitis during the study period. Conclusions: Incidence of acute pancreatitis in Iceland has not increased significantly compared with a study undertaken 10 years ago. Particularly, the incidence of alcohol induced pancreatitis has not increased despite increased alcohol consumption in Iceland. Acute pancreatitis in a population based setting had an overall good prognosis.
Christopher J. Secombes - One of the best experts on this subject based on the ideXlab platform.
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A recombinant CHSE-214 cell line expressing an Mx1 promoter-reporter system responds to both interferon type I and type II from salmonids and represents a versatile tool to study the IFN-system in teleost fish.
Fish & shellfish immunology, 2007Co-Authors: Jorunn B. Jørgensen, Audny Johansen, Marit N. Hegseth, Jun Zou, Børre Robertsen, Bertrand Collet, Christopher J. SecombesAbstract:Abstract A transgenic cell line for the detection of salmon interferons (IFNs) has been established. It is based on a CHSE-214 cell line containing a reporter construct expressing firefly luciferase under the control of the rainbow trout promoter for the IFN-induced Mx1 gene. This cell line, named CHSE-Mx10, showed IFN-induced luciferase expression after more than 80 passages, confirming the stability of this cell line. Interestingly, the Mx promoter was shown to respond to both salmon IFN-α/β and trout IFN-γ in a dose-dependent manner, while there was no response to TNF-α and IL-1β. IFN-α/β activity could be measured at a range of 9–150 U/ml, and IFN-γ showed activity between 10 and 100 ng/ml. The reproducibility of both responses was good. The CHSE-Mx10 reporter system constitutes a versatile tool to study the induction and regulation of IFN signaling in teleost fish. A preliminary study presented herein suggests that both infectious Pancreas Necrosis virus (IPNV) and salmon Pancreas disease virus (SPDV) may block activation of the Mx promoter in CHSE-Mx10 stimulated with IFN-α/β.
Johan F. Lange - One of the best experts on this subject based on the ideXlab platform.
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Late development of Pancreas Necrosis during acute pancreatitis: an underestimated phenomenon associated with high morbidity and mortality.
Pancreas, 2007Co-Authors: Niels A.t. Wijffels, Laurens A. Van Walraven, Peter J.a. Ophof, Wim C. J. Hop, Erwin Van Der Harst, Johan F. LangeAbstract:Objective: The purpose of this study is to assess the prognostic significance of late parenchymal Pancreas Necrosis as observed on serial contrast-enhanced computed tomographic (CT) scan. Methods: Eighty-four patients with acute necrotizing pancreatitis were included. All initial CT scans were examined on complete contrast enhancement of the Pancreas parenchyma (viable Pancreas) or incomplete enhancement indicating parenchymal Necrosis of the Pancreas (PN). Methods: Secondly, all serial CT scans were evaluated to investigate whether late PN occurred in the group with a viable Pancreas on initial CT scan. Characteristics of this group were evaluated. Results: Thirteen patients showed signs of PN on initial CT scan. Late Necrosis occurred in 5 patients. The average hospital stay in this subgroup was 46.7 days; complication rate, 100%; and mortality, 40%. Results: A significant difference in hospital stay (average of 45.1 days vs 24.3 days; P = 0.003), complication rate (72% vs 33%; P = 0.006), and mortality (28% vs 6%; P = 0.019) was found when the group with eventual PN was compared with the group with no signs of PN (on initial and serial CT). Conclusions: This study shows a significant increase in hospital stay, complication rate, and mortality when eventual PN is shown on CT scan. Patients with late PN especially have a poor prognosis.
Mehak Idrees - One of the best experts on this subject based on the ideXlab platform.
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Sa1382 Differences in Outcomes Among Patients With Post-ERCP Pancreatitis and Alcohol-Induced Acute Pancreatitis
Gastroenterology, 2013Co-Authors: Mehak Idrees, Ayesha Kamal, Izzah Vasim, Eun Ji Shin, Venkata S. Akshintala, Ali Kord Valeshabad, Payal Saxena, Vikesh K. Singh, Anne Marie Lennon, Marcia I. CantoAbstract:G A A b st ra ct s during the first 4 days or .120 mg/L during the first week. Six patients had severe complications, three patients had Pancreas Necrosis, two had pseudocysts and one developed renal failure. One of those patients was admitted to intensive care unit. No patient died of acute pancreatitis during the study period. Conclusions: Incidence of acute pancreatitis in Iceland has not increased significantly compared with a study undertaken 10 years ago. Particularly, the incidence of alcohol induced pancreatitis has not increased despite increased alcohol consumption in Iceland. Acute pancreatitis in a population based setting had an overall good prognosis.
