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Abdullah Saglam - One of the best experts on this subject based on the ideXlab platform.
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Effect of human Pancreatic Juice and bile on the tensile strength of suture materials
American Journal of Surgery, 2004Co-Authors: M.a. Tolga Muftuoglu, Erkan Ozkan, Abdullah SaglamAbstract:Abstract Background Several suture materials are used for pancreatojejunal anastomosis. In this study, we tested the durability of these suture materials in human Pancreatic Juice and bile. Methods Plain and chromic catgut, polyglactin 910, polyglycolic acid, polydioxanone, polypropylene, and silk sutures were incubated in Pancreatic Juice and bile that was collected from patients. Fifteen samples of each type of suture material were placed in human Juices for 1, 3, and 7 days. Tensile strengths were measured with a tensionmeter. Results Plain and chromic catgut disintegrated in Pancreatic Juice and Pancreatic Juice plus bile mixture. Polyglycolic acid and polyglactin 910 suture materials were vulnerable to Pancreatic Juice within 7 days. Polydioxanone retained most of its initial strength in Pancreatic Juice and bile. Polypropylene and silk retained 84% and 92% of their initial strength, respectively. Conclusions We found that polidioxanone was the strongest suture material in Pancreatic Juice.
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Scientific paper Effect of human Pancreatic Juice and bile on the tensile strength of suture materials
2004Co-Authors: M.a. Tolga Muftuoglu, Erkan Ozkan, Abdullah SaglamAbstract:Background: Several suture materials are used for pancreatojejunal anastomosis. In this study, we tested the durability of these suture materials in human Pancreatic Juice and bile. Methods: Plain and chromic catgut, polyglactin 910, polyglycolic acid, polydioxanone, polypropylene, and silk sutures were incubated in Pancreatic Juice and bile that was collected from patients. Fifteen samples of each type of suture material were placed in human Juices for 1, 3, and 7 days. Tensile strengths were measured with a tensionmeter. Results: Plain and chromic catgut disintegrated in Pancreatic Juice and Pancreatic Juice plus bile mixture. Polyglycolic acid and polyglactin 910 suture materials were vulnerable to Pancreatic Juice within 7 days. Polydioxanone retained most of its initial strength in Pancreatic Juice and bile. Polypropylene and silk retained 84% and 92% of their initial strength, respectively. Conclusions: We found that polidioxanone was the strongest suture material in Pancreatic Juice. © 2004 Excerpta Medica, Inc. All rights reserved.
Hiroyuki Uehara - One of the best experts on this subject based on the ideXlab platform.
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in situ telomerase activity in Pancreatic Juice may discriminate Pancreatic cancer from other Pancreatic diseases
Pancreas, 2008Co-Authors: Hiroyuki Uehara, Akihiko Nakaizumi, Akemi Takenaka, Hiroyasu Iishi, Hiroaki Ohigashi, Hidetoshi Eguchi, Osamu IshikawaAbstract:OBJECTIVES Detection of telomerase activity in Pancreatic Juice samples by telomeric repeat amplification protocol (TRAP) has been shown to be a useful diagnostic test for Pancreatic cancer. However, some investigations suggested that the activity was not specific to Pancreatic Juice from Pancreatic cancer. In the present study using an in situ TRAP assay, we investigated the specificity of telomerase activity in Pancreatic Juice for Pancreatic cancer. METHODS Pancreatic Juice were obtained endoscopically at endoscopic retrograde cholangiopancreatography from 10 patients with Pancreatic cancer, 4 with intraductal papillary neoplasm, and 3 with normal pancreas. In situ telomerase activity in Pancreatic Juice samples was examined by in situ TRAP assay and was compared with the results of cytological examination. RESULTS Of 10 samples from Pancreatic cancer, high telomerase activity in situ was detected in 9 and cancer cells in 7. Of 4 samples from intraductal papillary neoplasm, low telomerase activity in situ and mild atypical cells were detected in 2. Telomerase activity in situ was detected in none of 3 samples from normal pancreas. CONCLUSIONS Detection of in situ telomerase activity in Pancreatic Juice might help discriminate Pancreatic cancer from other Pancreatic diseases.
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CYTOLOGICAL CRITERIA OF Pancreatic Juice IN INTRADUCTAL PAPILLARY MUCINOUS NEOPLASMS
Digestive Endoscopy, 2006Co-Authors: Akihiko Nakaizumi, Akemi Takenaka, Rena Takakura, Hiroyuki UeharaAbstract:Although it is not always easy to distinguish benign from malignant intraductal papillary mucinous neoplasms of the pancreas by the imaging techniques, the cytological examination of Pancreatic Juice is a useful method to differentiate them. Therefore, the present paper describes the authors’ cytological criteria of Pancreatic Juice in intraductal papillary mucinous neoplasms.
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Diagnosis of Pancreatic cancer by K-ras point mutation and cytology of Pancreatic Juice.
The American journal of gastroenterology, 1996Co-Authors: Hiroyuki Uehara, Akemi Takenaka, Nakaizumi A, Miyako Baba, Hiroyasu Iishi, Masaharu Tatsuta, Kitamura T, Hiroaki Ohigashi, Osamu Ishikawa, Shingo IshiguroAbstract:Objectives : Recently, it was reported that detection of K-ras point mutation at codon 12 in Pancreatic Juice is an objective method for the diagnosis of Pancreatic cancer, but a few reports have suggested that this might represent an early event in Pancreatic oncogenesis. In the present study we examined, in various patients, the occurrence of K-ras codon 12 point mutation in Pancreatic Juice and compared it with Pancreatic Juice cytology, which is also a reliable diagnostic method. Methods : Pancreatic Juice was obtained endoscopically from patients with various Pancreatic disorders and those without definite diseases, and was examined cytologically and for the occurrence of K-ras codon 12 point mutation. The K-ras gene was amplified by polymerase chain reaction (PCR) and the mutation at codon 12 (GGT → GAT) was examined by slot blot hybridization analysis. Results : K-ras point mutation at codon 12 was detected in seven of 14 (50%) Pancreatic cancers, in four of 10 (40%) mucin-producing tumors, in four of 13 (31%) chronic pancreatitis, and in two of 10 (20%) pancreas without definite disorders. K-ras point mutation was detected in nine of 18 (50%) Pancreatic Juice samples containing cancer cells, in eight of 18 (44%) Pancreatic Juice samples containing atypical cells, but in none of such samples containing only normal cells. Conclusion : Cancer cells were detected from Pancreatic cancer exclusively, but K-ras point mutation at codon 12 was detected in Pancreatic Juice, not only from Pancreatic cancer, but also from other diseases.
Mads Grønborg - One of the best experts on this subject based on the ideXlab platform.
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comprehensive proteomic analysis of human Pancreatic Juice
Journal of Proteome Research, 2004Co-Authors: Mads Grønborg, Charles J Yeo, Ralph H Hruban, Jakob Bunkenborg, Troels Z Kristiansen, Ole Norregaard Jensen, Anirban MaitraAbstract:Proteomic technologies provide an excellent means for analysis of body fluids for cataloging protein constituents and identifying biomarkers for early detection of cancers. The biomarkers currently available for Pancreatic cancer, such as CA19-9, lack adequate sensitivity and specificity contributing to late diagnosis of this deadly disease. In this study, we carried out a comprehensive characterization of the “Pancreatic Juice proteome” in patients with Pancreatic adenocarcinoma. Pancreatic Juice was first fractionated by 1-dimensional gel electrophoresis and subsequently analyzed by liquid chromatography tandem mass spectrometry (LC−MS/MS). A total of 170 unique proteins were identified including known Pancreatic cancer tumor markers (e.g., CEA, MUC1) and proteins overexpressed in Pancreatic cancers (e.g., hepatocarcinoma−intestine−pancreas/pancreatitis-associated protein (HIP/PAP) and lipocalin 2). In addition, we identified a number of proteins that have not been previously described in Pancreatic jui...
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Proteomics of human Pancreatic Juice
Proteomics of Human Body Fluids, 1Co-Authors: Mads Grønborg, Anirban Maitra, Akhilesh PandeyAbstract:Pancreatic Juice has recently been characterized in detail using proteomic methods. The cataloging of proteins from healthy individuals and those diagnosed with Pancreatic cancer has revealed the presence of a number of proteins in Pancreatic Juice that could serve as potential biomarkers for cancer. Because obtaining Pancreatic Juice is not trivial, it is possible that these biomarkers can be detected in serum using more sensitive methods like ELISA. Here, we discuss the protein constituents of Pancreatic Juice with special reference to cancer biomarkers.
Stefan Pierzynowski - One of the best experts on this subject based on the ideXlab platform.
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Effect of the Antibacterial Activity of Pig Pancreatic Juice on Human Multiresistant Bacteria
Pancreas, 2004Co-Authors: Danuta Kruszewska, Åsa Ljungh, Sean O. Hynes, Stefan PierzynowskiAbstract:Introduction: The role of the exocrine pancreas in regulating gut microflora colonization is unclear. The main objective in the current study was to assess the effect of Pancreatic fluid on the growth of pathogenic bacteria and fungi. Methods: The antibacterial activity of pure pig Pancreatic Juice collected from catheterized, healthy, conscious, and anesthetized pigs was investigated with multiresistant microbial isolates and nonpathogenic strains. Studies were performed on pathogenic bacterial and fungi as well as lactic acid bacteria and reference strains. Results: Pancreatic Juice was effective (P < 0.01) against multidrug resistant bacterial pathogens, whereas lactic acid bacteria were insensitive. The antibacterial action was independent of Pancreatic Juice proteolytic activity. The in vitro antibacterial properties of Pancreatic Juice last for several hours. Data suggest that broth composition may modulate the intensity of Pancreatic Juice antibacterial activity. Conclusions: Pancreatic Juice antibacterial activity may be an important factor in limiting the colonization of pathogenic bacteria in the gastrointestinal tract. We postulate that observed antibacterial activity of the Pancreatic Juice could play an important role as one of the factors of innate immunity. (Less)
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Pancreatic Juice protects gut from pathogenic bacteria
2003Co-Authors: Danuta Kruszewska, Åsa Ljungh, Stefan PierzynowskiAbstract:The role of the exocrine pancreas in regulating gut microflora colonization is unclear. The main objective in the present study was to assess the affect of Pancreatic fluid on the growth of pathogenic bacteria and fungi. Pancreatic Juice samples used in the study were obtained from nine eight-week old weaned pigs in which catheters were implanted in the Pancreatic duct. The antibacterial activity of pure pig Pancreatic Juice collected from healthy, conscious and also anaesthetized pigs was investigated with multi-resistant microbial isolates and non-pathogenic strains. Studies were performed on 23 bacterial and 2 Candida albicans isolates, including 4 lactic acid bacteria (LAB) and 3 reference strains. Pancreatic Juice was effective (p
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Electrophoretic separation of proteolytic enzymes in Pancreatic Juice collected with the pouch or catheter method.
International journal of pancreatology : official journal of the International Association of Pancreatology, 1997Co-Authors: Vince M. Gabert, Mette S. Jensen, Björn Weström, Stefan PierzynowskiAbstract:Conclusion The results of this study demonstrated that proteolytic enzymes in Pancreatic Juice from pigs prepared with the pouch method (PM) were nearly fully active or were fully active. When activation with enterokinase was carried out further inactivation and/or breakdown occurred for chymotrypsin C and cathodal trypsin. In addition, some inactivation and/or breakdown of proteolytic enzymes in Pancreatic Juice occurred during collection of Pancreatic Juice from PM pigs.
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Comparative study of antibacterial activity of Pancreatic Juice in six mammalian species.
Pancreas, 1993Co-Authors: Stefan Pierzynowski, Peeyush Sharma, Jerzy Sobczyk, Stanislaw Garwacki, W. Barej, Björn WeströmAbstract:A comparative study of antibacterial activity of Pancreatic Juice was conducted on six mammalian species. Pancreatic Juice collections were conducted as acute (rabbit, guinea pig, rat) and chronic (pig, sheep, cattle) experiments, in the former before and after stimulation [cholecystokinin (CCK) and secretin] and in the latter under basal conditions alone. Antibacterial activity was tested on Micrococcus pyogenes and compared with that of neomycin. The samples were tested under normal conditions and after heating and dilution. The Pancreatic Juice of rat showed no activity against Micrococcus pyogenes. The antibacterial activity of rabbit and guinea pig Pancreatic Juice under basal conditions was similar within the group but significantly higher than that of pig, sheep and cattle which also did not differ significantly within the group. On stimulation with CCK and secretin, no significant change could be observed in the potency of antimicrobial activity of Pancreatic Juice in the rabbit and guinea pig. The antibacterial activity remained unchanged after heating to 65 degrees C and upon dilution to 1:10.
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Influence of feeding regimen and postnatal developmental stages on antibacterial activity of Pancreatic Juice.
International journal of pancreatology : official journal of the International Association of Pancreatology, 1992Co-Authors: Stefan Pierzynowski, Sharma P, Sobczyk J, Garwacki S, Barej WAbstract:Antibacterial activity of Pancreatic Juice in the pig (n = 8) was investigated during early postnatal development and in cattle (n = 6) receiving a different feeding regimen. For Pancreatic Juice collection, a catheter was surgically implanted in the Pancreatic duct. Reintroduction of Pancreatic Juice was achieved through a T-shaped cannula in the duodenum. Pancreatic Juice was collected for 30 min in all cases. In piglets, collections were carried out at 2, 5-6, and 7-10 wk of age, and in cattle, after a standard meal, 48 h starvation, and following 24 h intraduodenal glucose infusion. Antibacterial activity was tested on Micrococcus Pyogenes strain ATTC 6538P by disc agar diffusion technique using nonactivated Pancreatic Juice, before and after heat treatment for 15 min at 65 and 100°C, respectively.
M.a. Tolga Muftuoglu - One of the best experts on this subject based on the ideXlab platform.
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Effect of human Pancreatic Juice and bile on the tensile strength of suture materials
American Journal of Surgery, 2004Co-Authors: M.a. Tolga Muftuoglu, Erkan Ozkan, Abdullah SaglamAbstract:Abstract Background Several suture materials are used for pancreatojejunal anastomosis. In this study, we tested the durability of these suture materials in human Pancreatic Juice and bile. Methods Plain and chromic catgut, polyglactin 910, polyglycolic acid, polydioxanone, polypropylene, and silk sutures were incubated in Pancreatic Juice and bile that was collected from patients. Fifteen samples of each type of suture material were placed in human Juices for 1, 3, and 7 days. Tensile strengths were measured with a tensionmeter. Results Plain and chromic catgut disintegrated in Pancreatic Juice and Pancreatic Juice plus bile mixture. Polyglycolic acid and polyglactin 910 suture materials were vulnerable to Pancreatic Juice within 7 days. Polydioxanone retained most of its initial strength in Pancreatic Juice and bile. Polypropylene and silk retained 84% and 92% of their initial strength, respectively. Conclusions We found that polidioxanone was the strongest suture material in Pancreatic Juice.
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Scientific paper Effect of human Pancreatic Juice and bile on the tensile strength of suture materials
2004Co-Authors: M.a. Tolga Muftuoglu, Erkan Ozkan, Abdullah SaglamAbstract:Background: Several suture materials are used for pancreatojejunal anastomosis. In this study, we tested the durability of these suture materials in human Pancreatic Juice and bile. Methods: Plain and chromic catgut, polyglactin 910, polyglycolic acid, polydioxanone, polypropylene, and silk sutures were incubated in Pancreatic Juice and bile that was collected from patients. Fifteen samples of each type of suture material were placed in human Juices for 1, 3, and 7 days. Tensile strengths were measured with a tensionmeter. Results: Plain and chromic catgut disintegrated in Pancreatic Juice and Pancreatic Juice plus bile mixture. Polyglycolic acid and polyglactin 910 suture materials were vulnerable to Pancreatic Juice within 7 days. Polydioxanone retained most of its initial strength in Pancreatic Juice and bile. Polypropylene and silk retained 84% and 92% of their initial strength, respectively. Conclusions: We found that polidioxanone was the strongest suture material in Pancreatic Juice. © 2004 Excerpta Medica, Inc. All rights reserved.
