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Katherine D Mosquera - One of the best experts on this subject based on the ideXlab platform.
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chagas vectors Panstrongylus chinai del ponte 1929 and Panstrongylus howardi neiva 1911 chromatic forms or true species
Parasites & Vectors, 2020Co-Authors: Francisco Panzera, Jeanpierre Dujardin, Katherine D Mosquera, Anita G Villacis, Cesar A Yumiseva, Sebastian Pita, Soledad Santillanguayasamin, Marco I OrozcoAbstract:Chagas disease is a parasitic infection transmitted by “kissing bugs” (Hemiptera: Reduviidae: Triatominae) that has a huge economic impact in Latin American countries. The vector species with the upmost epidemiological importance in Ecuador are Rhodnius ecuadoriensis (Lent & Leon, 1958) and Triatoma dimidiata (Latreille, 1811). However, other species such as Panstrongylus howardi (Neiva, 1911) and Panstrongylus chinai (Del Ponte, 1929) act as secondary vectors due to their growing adaptation to domestic structures and their ability to transmit the parasite to humans. The latter two taxa are distributed in two different regions, they are allopatric and differ mainly by their general color. Their relative morphological similarity led some authors to suspect that P. chinai is a melanic form of P. howardi. The present study explored this question using different approaches: antennal phenotype; geometric morphometrics of heads, wings and eggs; cytogenetics; molecular genetics; experimental crosses; and ecological niche modeling. The antennal morphology, geometric morphometrics of head and wing shape and cytogenetic analysis were unable to show distinct differences between the two taxa. However, geometric morphometrics of the eggs, molecular genetics, ecological niche modeling and experimental crosses including chromosomal analyses of the F1 hybrids, in addition to their coloration and current distribution support the hypothesis that P. chinai and P. howardi are separate species. Based on the evidence provided here, P. howardi and P. chinai should not be synonymized. They represent two valid, closely related species.
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life cycle feeding and defecation patterns of Panstrongylus chinai hemiptera reduviidae triatominae under laboratory conditions
Journal of Medical Entomology, 2016Co-Authors: Katherine D Mosquera, Anita G Villacis, Mario J GrijalvaAbstract:Chagas disease is caused by the protozoan Trypanosoma cruzi . Panstrongylus chinai (Del Ponte) is highly domiciliated in the Peruvian and Ecuadorian Andes and has been found naturally infected with T. cruzi . The objective of this study was to describe the life cycle, feeding, and defecation patterns of P. chinai in the Loja province within southern Ecuador. To characterize its life cycle, a cohort of 70 individuals was followed from egg to adult. At each stage of development, prefeeding time, feeding time, weight of ingested meal, proportional weight increase, and the time to the first defecation were recorded. Panstrongylus chinai completed its development in 371.4 ± 22.3 d, (95% CI 355.4–387.4), which means that it is likely a univoltine species. Prefeeding time, feeding time, and weight of ingested meal increased as individuals developed through nymphal stages. Moreover, time to first defecation was shortest in the early nymphal stages, suggesting higher vector potential in the early developmental stages. Data obtained in this study represent an important advance in our knowledge of the biology of P. chinai , which should be considered as a secondary Chagas disease vector species in the Andean valleys of Loja (Ecuador) and in the north of Peru, and included in entomological surveillance programs.
Anita G Villacis - One of the best experts on this subject based on the ideXlab platform.
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chagas vectors Panstrongylus chinai del ponte 1929 and Panstrongylus howardi neiva 1911 chromatic forms or true species
Parasites & Vectors, 2020Co-Authors: Francisco Panzera, Jeanpierre Dujardin, Katherine D Mosquera, Anita G Villacis, Cesar A Yumiseva, Sebastian Pita, Soledad Santillanguayasamin, Marco I OrozcoAbstract:Chagas disease is a parasitic infection transmitted by “kissing bugs” (Hemiptera: Reduviidae: Triatominae) that has a huge economic impact in Latin American countries. The vector species with the upmost epidemiological importance in Ecuador are Rhodnius ecuadoriensis (Lent & Leon, 1958) and Triatoma dimidiata (Latreille, 1811). However, other species such as Panstrongylus howardi (Neiva, 1911) and Panstrongylus chinai (Del Ponte, 1929) act as secondary vectors due to their growing adaptation to domestic structures and their ability to transmit the parasite to humans. The latter two taxa are distributed in two different regions, they are allopatric and differ mainly by their general color. Their relative morphological similarity led some authors to suspect that P. chinai is a melanic form of P. howardi. The present study explored this question using different approaches: antennal phenotype; geometric morphometrics of heads, wings and eggs; cytogenetics; molecular genetics; experimental crosses; and ecological niche modeling. The antennal morphology, geometric morphometrics of head and wing shape and cytogenetic analysis were unable to show distinct differences between the two taxa. However, geometric morphometrics of the eggs, molecular genetics, ecological niche modeling and experimental crosses including chromosomal analyses of the F1 hybrids, in addition to their coloration and current distribution support the hypothesis that P. chinai and P. howardi are separate species. Based on the evidence provided here, P. howardi and P. chinai should not be synonymized. They represent two valid, closely related species.
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life cycle feeding and defecation patterns of Panstrongylus chinai hemiptera reduviidae triatominae under laboratory conditions
Journal of Medical Entomology, 2016Co-Authors: Katherine D Mosquera, Anita G Villacis, Mario J GrijalvaAbstract:Chagas disease is caused by the protozoan Trypanosoma cruzi . Panstrongylus chinai (Del Ponte) is highly domiciliated in the Peruvian and Ecuadorian Andes and has been found naturally infected with T. cruzi . The objective of this study was to describe the life cycle, feeding, and defecation patterns of P. chinai in the Loja province within southern Ecuador. To characterize its life cycle, a cohort of 70 individuals was followed from egg to adult. At each stage of development, prefeeding time, feeding time, weight of ingested meal, proportional weight increase, and the time to the first defecation were recorded. Panstrongylus chinai completed its development in 371.4 ± 22.3 d, (95% CI 355.4–387.4), which means that it is likely a univoltine species. Prefeeding time, feeding time, and weight of ingested meal increased as individuals developed through nymphal stages. Moreover, time to first defecation was shortest in the early nymphal stages, suggesting higher vector potential in the early developmental stages. Data obtained in this study represent an important advance in our knowledge of the biology of P. chinai , which should be considered as a secondary Chagas disease vector species in the Andean valleys of Loja (Ecuador) and in the north of Peru, and included in entomological surveillance programs.
Francois Noireau - One of the best experts on this subject based on the ideXlab platform.
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New Discoveries of Sylvatic Triatoma infestans (Hemiptera: Reduviidae) Throughout the Bolivian Chaco
American Journal of Tropical Medicine and Hygiene, 2012Co-Authors: Etienne Waleckx, Stéphanie Depickère, Renata Salas, Claudia Aliaga, Marcelo Monje, Hiber Calle, Rosio Buitrago, Francois Noireau, Simone Frederique BreniereAbstract:Sylvatic populations of Triatoma infestans might be involved in the recolonization of human dwellings. We report here the discoveries of new T. infestans sylvatic foci in the Bolivian Chaco. Eighty-one triatomines were caught, 38 of which were identified as T. infestans. Triatoma sordida and Panstrongylus geniculatus were the other species collected. One T. infestans and one T. sordida were infected with Trypanosoma cruzi TcI; one T. infestans was infected with TcII. These discoveries add to the debate on the geographic distribution of sylvatic T. infestans populations, the geographic origin of the species, and the epidemiological role of these populations.
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Wild Habitat and Related Fauna of Panstrongylus lutzi (Reduviidae, Triatominae)
Journal of Medical Entomology, 2003Co-Authors: Artur G. Dias-lima, Diego Menezes, Ítalo A. Sherlock, Francois NoireauAbstract:Abstract Although Panstrongylus lutzi (Neiva and Pinto) is currently the fifth most frequent species of Triatominae captured in artificial structures in Brazil, its silvatic habitat remains unknown. A survey of its natural ecotopes was performed in an area of Bahia State. P. lutzi nymphal instars and adults were detected in burrows of Dasypodidae. Silvatic habitat of four other endemic triatomine species of the caatinga (Triatoma pseudomaculata Correa and Espinola, Triatoma brasiliensis Neiva, Rhodnius neglectus Lent, and Psammolestes tertius Lent and Jurberg) was also recorded.
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nuclear rdna its 2 sequences reveal polyphyly of Panstrongylus species hemiptera reduviidae triatominae vectors of trypanosoma cruzi
Infection Genetics and Evolution, 2002Co-Authors: Antonio Marcilla, Francois Noireau, M D Bargues, Fernando Abadfranch, Michael A Miles, Francisco Panzera, R U Carcavallo, Cleber Galvao, Jose Jurberg, Jeanpierre DujardinAbstract:Panstrongylus species are widely distributed throughout the Americas, where they act as vectors of Trypanosoma cruzi, agent of Chagas disease. Their intraspecific relationships, taxonomic position and phylogeny in relation to other Triatomini were explored using ribosomal DNA (rDNA) internal transcribed spacer 2 (ITS-2) sequence polymorphisms and maximum parsimony, distance and maximum likelihood analyses of 10 populations representing six species of the genus (P. megistus, P. geniculatus, P. rufotuberculatus, P. lignarius, P. herreri and P. chinai). At the subspecific level, P. megistus appeared more homogeneous than P. rufotuberculatus and P. geniculatus (both with broader distribution). Several dinucleotide microsatellites were detected in the sequences of given species. Many of these microsatellites (GC, TA, GT and AT) showed different number of repeats in different populations and thus, may be very useful for population differentiation and dynamics analyses in future studies. The sequences of P. lignarius (considered sylvatic) and P. herreri (a major disease vector in Peru) were identical, suggesting that these species should be synonymised. Intrageneric analysis showed a clear separation of P. rufotuberculatus, with closest relationships between P. geniculatus and P. chinai, and P. megistus occupying a separate branch. Genetic distances between Panstrongylus species (0.11585-0.22131) were higher than those between Panstrongylus and other Triatomini (16 species from central and North America and South America) (0.08617-0.11039). The distance between P. megistus and P. lignarius/herreri (0.22131) was the largest so far recorded in the tribe. The pronounced differences in length and nucleotide composition suggest a relatively old divergence of Panstrongylus species. P. rufotuberculatus was closer to Mesoamerican Triatoma, Meccus and Dipetalogaster species than to other Panstrongylus. All Panstrongylus clustered with the Mesoamerican clade; P. rufotuberculatus clustered with the phyllosoma complex and T. dimidiata, with D. maxima and T. barberi in a basal position. The rest of Panstrongylus appeared paraphyletically in the tree. This is evidence suggesting polyphyly within the genus Panstrongylus, whose species may be related to the ancestors giving rise to central and North American Triatomini.
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chagas disease in the amazon basin association of Panstrongylus geniculatus hemiptera reduviidae with domestic pigs
Journal of Medical Entomology, 1998Co-Authors: Vera Da Costa Valente, Francois Noireau, Sebastiao Aldo Da Silva Valente, Hernan J Carrasco, Michael A MilesAbstract:Just over 100 autochthonous cases of Chagas disease are reported from the Brazilian Amazon Basin. Panstrongylus geniculatus (Latreille) occurs throughout the region and is the known vector of Trypanosoma cruzi, principal zymodeme 3 (Z3) to the armadillo Dasypus novemcinctus. In the small riverine community of Furo do Rio Pau Grande, pigsties adjoining houses were heavily infested with P. geniculatus, which repeatedly attacked local inhabitants. Palm trees in the immediate vicinity were also infested. T. cruzi principal zymodeme 1 (Z1) was isolated from P. geniculatus, domestic pigs, and opossums, but no human infections were detected. The threat of endemic Chagas disease to the Amazon Basin from either domiciliation of local silvatic triatomine species, or from migration of domestic vectors, demands a program of vigilance and plans of action to eliminate household triatomine colonies.
Joao Aristeu Da Rosa - One of the best experts on this subject based on the ideXlab platform.
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genetic diversification of Panstrongylus geniculatus reduviidae triatominae in northern south america
PLOS ONE, 2019Co-Authors: Valentina Caicedogarzon, Fabian C Salgadoroa, Melissa Sanchezherrera, Carolina Hernandez, Luisa M Ariasgiraldo, Lineth Garcia, Gustavo Adolfo Vallejo, Omar Cantillo, Catalina Tovar, Joao Aristeu Da RosaAbstract:Triatomines are the vectors of Trypanosoma cruzi, the etiological agent of Chagas disease. Although Triatoma and Rhodnius are the most-studied vector genera, other triatomines, such as Panstrongylus, also transmit T. cruzi, creating new epidemiological scenarios. Panstrongylus has at least 13 reported species but there is limited information about its intraspecific genetic variation and patterns of diversification. Here, we begin to fill this gap by studying populations of P. geniculatus from Colombia and Venezuela and including other epidemiologically important species from the region. We examined the pattern of diversification of P. geniculatus in Colombia using mitochondrial and nuclear ribosomal data. Genetic diversity and differentiation were calculated within and among populations of P. geniculatus. Moreover, we constructed maximum likelihood and Bayesian inference phylogenies and haplotype networks using P. geniculatus and other species from the genus (P. megistus, P. lignarius, P. lutzi, P. tupynambai, P. chinai, P. rufotuberculatus and P. howardi). Using a coalescence framework, we also dated the P. geniculatus lineages. The total evidence tree showed that P. geniculatus is a monophyletic species, with four clades that are concordant with its geographic distribution and are partly explained by the Andes orogeny. However, other factors, including anthropogenic and eco-epidemiological effects must be investigated to explain the existence of recent geographic P. geniculatus lineages. The epidemiological dynamics in structured vector populations, such as those found here, warrant further investigation. Extending our knowledge of P. geniculatus is necessary for the accurate development of effective strategies for the control of Chagas disease vectors.
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occurrences of triatomines hemiptera reduviidae and first reports of Panstrongylus geniculatus in urban environments in the city of sao paulo brazil
Revista Do Instituto De Medicina Tropical De Sao Paulo, 2018Co-Authors: Walter Cerettijunior, Daniel Pagotto Vendrami, Joao Aristeu Da Rosa, Marco Otavio De Matosjunior, Aline Rimoldiribeiro, Julia Vono Alvarez, Sandro Marques, Agnaldo Nepomuceno Duarte, Rubens Antonio Da Silva, Mauro Toledo MarrelliAbstract:This note reports on occurrences of triatomine species in the city of Sao Paulo, Brazil, registered between 1988 and 2017. Records of triatomines captured in Sao Paulo are based on specimens received spontaneously from Health Surveillance Centers, Health Centers and Zoonosis Control Centers in the city as well as from citizens. Species were identified morphologically at the Public Health Entomology Laboratory, Faculty of Public Health, University of Sao Paulo, where the triatomines, which are vectors of Chagas disease, were tested for Trypanosoma cruzi infection. The first reported occurrence of triatomine bugs in urban Sao Paulo was in 1988. The specimen, which was captured in Jardim Sao Luiz district, was from the genus Panstrongylus and was registered as Panstrongylus sp. but was not sexed. Since this first recorded occurrence, the following species have been found in the city: Panstrongylus geniculatus (2 occurrences), P. megistus (15 occurrences), Triatoma infestans (1 occurrence) and T. sordida (3 occurrences). In this paper, the importance of reporting occurrences of triatomine bugs in the city of Sao Paulo, one of the largest metropolis in the world, is discussed with an emphasis on P. megistus. The occurrences discussed here indicate the importance of entomological surveillance for these vectors even in urban centers although the possibility of vector transmission of Chagas disease in these centers is very low.
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first report of Panstrongylus megistus hemiptera reduviidae triatominae in the state of acre and rondonia amazon brazil
Acta Tropica, 2018Co-Authors: Jader De Oliveira, Joao Aristeu Da Rosa, Gabriela Vieira De Souza Castro, Mariane Albuquerque Lima Ribeiro Castro, Janis Lunier De Souza, Claudio Rodrigues De Souza, Leandro Jose Ramos, Luis Marcelo Aranha CamargoAbstract:Abstract Introduction This article reports, for the first time, the occurrence of Panstrongylus megistus in the Brazilian Western Amazon. Methods Specimens of P. megistus were collected in the cities of Rio Branco, Acre and Extrema, Rondonia. Results The number of triatomine species in the State of Acre increased from eight to nine and in Rondonia from seven to eight. This was also the first report of P. megistus in the Brazilian Western Amazon. Conclusion The occurrence of P. megistus in the Western Amazon evidences an epidemiological alert, since it is an important vector of T. cruzi.
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homogeneidade cromossomica em diferentes populacoes de Panstrongylus megistus do brasil
Journal of Basic and Applied Pharmaceutical Sciencies, 2015Co-Authors: Kaio Cesar Chaboli Alevi, Joao Aristeu Da Rosa, Giovana Menezes Nunes, Maria Tercilia Vilela De Azeredo OliveiraAbstract:Os triatomineos sao insetos vetores do protozoario Trypanosoma cruzi, agente etiologico da doenca de Chagas. Existem seis especies de maior importância vetorial, a saber, Triatoma infestans, Panstrongylus megistus, T. brasiliensis, T. sordida, T. pseudomaculata e Rhodnius neglectus. P. megistus apresenta grande distribuicao geografica e ja foi coletada em 22 Estados brasileiros. Variacoes cromossomicas intraespecificas ja foram detectadas na subfamilia Triatominae. Assim, o presente trabalho teve como objetivo analisar as caracteristicas cromossomicas de P. megistus provenientes de sete diferentes Estados brasileiros (Rio Grande do Norte, Rio Grande do Sul, Parana, Sao Paulo, Minas Gerais, Bahia e Santa Catarina), por meio de tecnicas citogeneticas classicas. Todos os insetos analisados apresentaram as mesmas caracteristicas cromossomicas: 21 cromossomos, sistema de determinacao do sexo do tipo X1X2Y, tamanho relativo dos autossomos com pequena variacao, tamanho relativo dos cromossomos sexuais (Y>X1>X2), cromocentro na profase meiotica formado apenas pelos cromossomos sexuais e presenca de heterocromatina apenas no cromossomo sexual Y. Sendo assim, o presente trabalho apresenta dados evolutivos sobre as populacoes de P. megistus do Brasil, demonstrando que as populacoes de diferentes Estados brasileiros apresentam homogeneidade cromossomica. Palavras-chave: Citogenetica. Cariotipo. Bandamento C. Subfamilia Triatominae. ABSTRACT The triatomines are insects vectors of the protozoan Trypanosoma cruzi, etiologic agent of Chagas disease. There are six species of greatest importance vector, out more, Triatoma infestans, Panstrongylus megistus, T. brasiliensis, T. sordida, Rhodnius neglectus and T. pseudomaculata. P. megistus presents great geographic distribution and has been collected in 22 Brazilian states. Intraspecific chromosomal variations have been detected in Triatominae. Thus, the present study aimed to analyze the chromosomal characteristics of P. megistus from seven different Brazilian states (Rio Grande do Norte, Rio Grande do Sul, Parana, Sao Paulo, Minas Gerais, Bahia and Santa Catarina) by means of classical cytogenetic techniques. All insects analyzed showed the same chromosomal characteristics: 21 chromosomes, sex determination system the type X1X2Y, relative size of autosomes with little variation, relative size of the sex chromosomes (Y>X1>X2), chromocenter in meiotic prophase formed only by sex chromosomes and the presence of heterochromatin only in sex chromosome Y. Thus, this paper presents evolutionary data on the populations of P. megistus of Brazil demonstrating that populations from different Brazilian states have chromosomal homogeneity. Keywords: Cytogenetics. Karyotype. C-banding. Triatominae subfamily.
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multiple mitochondrial genes of some sylvatic brazilian triatoma non monophyly of the t brasiliensis subcomplex and the need for a generic revision in the triatomini
Infection Genetics and Evolution, 2014Co-Authors: Sueli Gardim, Jader De Oliveira, Daniela M Takiya, Carlos Eduardo Almeida, Renato Freitas De Araujo, Regina Maria Barretto Cicarelli, Joao Aristeu Da RosaAbstract:Multiple fragments of mitochondrial DNA genes (cytochrome b, cytochrome oxidase I, and 16S rDNA) were used to evaluate the phylogenetic relationships among Triatoma melanocephala, Triatoma tibiamaculata, Triatoma vitticeps, and other members of Triatoma brasiliensis subcomplex under a Bayesian framework and maximum parsimony criterion. With the addition of new sequences of T. tibiamaculata and T. vitticeps, Triatoma juazeirensis, Triatoma melanica and the newly sequenced T. melanocephala, the three first sylvatic species, T. melanocephala, T. tibiamaculata and T. vitticeps, were strongly recovered into a clade separate from the other with the remaining Triatoma species from South America, such as the members of T. brasiliensis subcomplex. Panstrongylus megistus was recovered as a sister to T. tibiamaculata, whereas T. vitticeps was a sister to T. melanocephala. This study revealed the non-monophyly of the T. brasiliensis subcomplex, and the polyphyly of Triatoma was reinforced by the placement of these three sylvatic species with Dipetalogaster, Meccus, Mepraia, and Panstrongylus. The results herein shown highlight the need of generic revision in Triatomini.
Rodrigo Pedro Soares - One of the best experts on this subject based on the ideXlab platform.
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interpopulation variability among Panstrongylus megistus hemiptera reduviidae from brazil
Journal of Medical Entomology, 2003Co-Authors: Rodrigo Pedro Soares, Francisco Panzera, S E Barbosa, Herton Helder Rocha Pires, Jean P Dujardin, Carina Margonari, Alvaro J Romanha, Pedro Marcos LinardiAbstract:Abstract Comparisons were made among Panstrongylus megistus (Burmeister) from three areas of Brazil (Bahia, Minas Gerais, and Santa Catarina), where populations differ with regard to their degree of association with human dwellings. The following characters were studied: morphology of the eggs and the male genitalia; morphometry of the head and thorax; isoenzyme profile; enzymatic activity of the ∝-glycerophosphate dehydrogenase (∝-GPDH); and cytogenetics. In general, differences were observed in the weight, diameter, and length of the egg among Bahia, Minas Gerais, and Santa Catarina populations. Differences were not observed in the architecture of the egg exochorion. The size of the median process of the pygophore of the male genitalia of individuals from Bahia differed from the other two populations. The Minas Gerais population presented the largest number of denticles in the endosome process. The morphometry of the head and thorax differentiated Santa Catarina from the Bahia and Minas Gerais populatio...
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experimental evidence for a demographic cline in Panstrongylus megistus populations
Memorias Do Instituto Oswaldo Cruz, 2001Co-Authors: S E Barbosa, Rodrigo Pedro Soares, Herton Helder Rocha Pires, Lileia DiotaiutiAbstract:The population biology of three populations of Panstrongylus megistus was compared to determine possible influence on the behaviour and epidemiological importance of this species. The results demonstrated differences in terms of egg eclosion time, nymphal mortality and development rates, and feeding and defaecation rates. These differences appeared to follow a geographical cline, primarily reflecting different degrees of adaptation to domestic habitats.
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alpha glycerophosphate dehydrogenase activity in flight muscles of triatomine bugs Panstrongylus megistus and triatoma sordida
Memorias Do Instituto Oswaldo Cruz, 2000Co-Authors: Rodrigo Pedro Soares, M M SantoroAbstract:The a-glycerophosphate dehydrogenase (a-GPDH) activity in flight muscles of Panstrongylus megistus and Triatoma sordida, vectors of Chagas disease in Brazil, was studied. Both species showed higher enzymatic activities in fliers than in non-fliers insects. T. sordida exhibited a higher proportion of flier insects than P. megistus. A possible role of a-GPDH on triatomines flight is discussed.
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differences in saliva composition among three brazilian populations of Panstrongylus megistus hemiptera reduviidae
Acta Tropica, 1999Co-Authors: Rodrigo Pedro Soares, Lileia Diotaiuti, S E Barbosa, Marcos H PereiraAbstract:Comparisons of electrophoretic profiles of triatomines (Panstrongylus megistus) from the Brazilian states of Bahia (BA), Minas Gerais (MG) and Santa Catarina (SC) revealed differences in the composition of the saliva between the three populations. A phenogram constructed on the basis of the most representative electrophoretic banding patterns allowed the specimens tested to be divided into two groups. The BA and SC populations could be completely separated while some individuals from MG could be placed in each group. The BA population presented fewer bands than the MG and SC ones. The populations studied also differed with regard to their degree of association with human habitations (sylvatic, peridomiciliary and/or intradomiciliary) and our results suggest that the proteinaceous composition of the saliva of the three distinct populations of triatomines could be related to the hosts each is likely to encounter.
