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Joao Aristeu Da Rosa - One of the best experts on this subject based on the ideXlab platform.
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characterization of female external genitalia and eggs of four south american species of the Triatoma laporte 1832 genus hemiptera reduviidae triatominae
Insects, 2021Co-Authors: Tiago Belintani, Cleber Galvao, Jader De Oliveira, Juliana Damieli Nascimento, Kaio Cesar Chaboli Alevi, Heloisa Pinotti, Estela Sassocerri, Joao Aristeu Da RosaAbstract:Triatoma is the most diversified and one of the most important genera from an epidemiological perspective. Given the difficulty in identifying some species of the Triatoma genus, morphological, histological, and morphometric studies were performed to provide new characters that make it possible to differentiate T. garciabesi, T. guasayana, T. patagonica, and T. sordida sensu stricto, triatomines that overlap geographically and have vector potential. Through the external female genitalia, as well as morphology, morphometry, and histology of eggshells, it was possible to discriminate the four species. In addition, this study reinforces the taxonomic validity of T. garciabesi and provides new data for discussion on systematic issues of T. guasayana and T. patagonica.
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Formal Assignation of the Kissing Bug Triatoma lecticularia (Hemiptera: Reduviidae: Triatominae) to the Genus ParaTriatoma
'MDPI AG', 2021Co-Authors: Vinicius Fernandes De Paiva, Cleber Galvao, Jader De Oliveira, Silvia Andrade Justi, José Manuel Ayala Landa, Joao Aristeu Da RosaAbstract:The subfamily Triatominae (Hemiptera: Reduviidae) comprises hematophagous insects that are vectors of Chagas disease; including species assigned to the genera Triatoma and ParaTriatoma. Initial examination of Triatoma lecticularia revealed the hirsuteness covering the entire body—a characteristic and striking feature of members of the genus ParaTriatoma—and a systematic study revealed several other morphological characters that are in diagnostic alignment with ParaTriatoma. Based on the examination of several specimens (including the lectotype), and with the additional support of molecular and cytogenetic data, we propose the formal transferal of Triatoma lecticularia (Stål, 1859) into the genus ParaTriatoma with the resulting new combination: ParaTriatoma lecticularia (Stål, 1859) comb. nov. (Hemiptera: Reduviidae: Triatominae)
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molecular cytotaxonomy of the Triatoma brasiliensis species subcomplex hemiptera triatominae
Acta Tropica, 2020Co-Authors: Kaio Cesar Chaboli Alevi, Jader De Oliveira, Joao Aristeu Da Rosa, Isadora De Freitas Bittinelli, Luiza Maria Grzyb Delgado, Fernanda Fernandez Madeira, Mauricio Lilioso, Elaine Follyramos, Maria Tercilia Vilela De AzeredooliveiraAbstract:Abstract The Triatoma genus is paraphyletic, and its species are grouped into complexes and subcomplexes. Given the fact that species that make up a given subcomplex generally share chromosomal traits, we analyzed the distribution of AT- and CG-rich DNA of the T. brasiliensis species subcomplex, in order to establish affinities among members of the T. brasiliensis subcomplex based on chromatin and chromosome traits and develop an identification key for the four monophyletic Triatoma subcomplexes from South America. All species exhibited a CG-rich X sex chromosome and autosomes, as well as an AT-rich Y sex chromosome. This feature can be used as a diagnostic characteristic to determine whether a given species is a member of the T. brasiliensis subcomplex, because it enables the differentiation of these species from all Triatoma of South America. Thus, we confirmed the chromosomal relationship of the T. brasiliensis species subcomplex and developed a dichotomous key based on the chromocenter to differentiate the species from this subcomplex from the other monophyletic Triatoma subcomplexes from South America.
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first report of Triatoma sordida stal 1859 hemiptera reduviidae triatominae in the state of acre and brazilian western amazon
Revista Da Sociedade Brasileira De Medicina Tropical, 2018Co-Authors: Leandro Jose Ramos, Janis Lunier De Souza, Claudio Rodrigues De Souza, Renildo Moura Da Cunha, Jader De Oliveira, Joao Aristeu Da Rosa, Luis Marcelo Aranha Camargo, Dionatas Ulises De Oliveira MeneguettiAbstract:INTRODUCTION: The occurrence of Triatoma sordida in the Brazilian Western Amazon is reported for the first time. METHODS: Triatoma sordida specimens were collected from a Gallus gallus nest in a peridomiciliary area of Senador Guiomard municipality in the state of Acre. RESULTS: The number of triatomine species in Acre increased from six to seven with this first report of T. sordida in the Brazilian Western Amazon. CONCLUSIONS: The occurrence of T. sordida in Acre is of concern since it is among the most captured triatomines in peridomiciliary environments in Brazil, and carries a high potential for vector transmission.
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chromosomal characteristics and distribution of constitutive heterochromatin in the matogrossensis and rubrovaria subcomplexes
Infection Genetics and Evolution, 2015Co-Authors: Kaio Cesar Chaboli Alevi, Jader De Oliveira, Joao Aristeu Da Rosa, Jose Jurberg, Felipe Ferraz Figueiredo Moreira, Maria Tercilia Vilela De AzeredooliveiraAbstract:Since 1966 the triatomines were grouped in complexes and specific subcomplexes. Although the complex and subcomplexes not have taxonomic importance, should be monophyletic groups and cytogenetic tools have proved to be of great importance to characterize these species groupings. Based on this, this paper aims to describe the chromosomal characteristics and heterochromatic pattern of Matogrossensis and Rubrovaria subcomplexes, in order to contribute to the taxonomic and evolutionary relationships of these vectors. In this study, at least three males from each species (Triatoma baratai, Triatoma costalimai, Triatoma guazu, Triatoma jurbergi, Triatoma matogrossensis, Triatoma vandae, Triatoma williami, Triatoma carcavalloi, Triatoma circummaculata, Triatoma klugi, Triatoma pintodiasi and Triatoma rubrovaria) were analyzed by means analyzed by means of cytogenetic techniques of C-banding. All species showed the same cytogenetic characteristics: 22 chromosomes, low variation in the size of autosomes, sex chromosome Y larger than X, initial prophase composed of only one heterochromatic chromocenter formed by the sex chromosomes X and Y (except for T. pintodiasi that presented the sex chromosomes individualized during all stages of prophase) and presence of constitutive heterochromatin restricted to sex chromosome Y. These characteristics, although common to Matogrossensis and Rubrovaria subcomplexes allow to distinguish these species of species grouped in most of South America subcomplexes, as Brasiliensis, Maculata, Sordida and Insfestans. Thus, the cytogenetic analysis was of extreme importance to differentiate both subcomplexes of the other subcomplexes of South America. However, probably due to evolutionary proximity existing between these subcomplexes was not possible to observar species differences that make up the Matogrossensis subcomplex of the Rubrovaria subcomplex. Therefore, we emphasize that new comparative analyzes, as experimental hybrid crosses and molecular cytogenetic analysis are necessary to clarify the evolutionary relationship between these important subcomplexes of vectors.
Francois Noireau - One of the best experts on this subject based on the ideXlab platform.
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cryptic speciation in the Triatoma sordida subcomplex hemiptera reduviidae revealed by chromosomal markers
Parasites & Vectors, 2015Co-Authors: Francisco Panzera, Sebastian Pita, Cleber Galvao, Tamara Chavez, Lourdes Cardozo Tellez, Yanina Panzera, Julieta Nattero, Antonieta Rojas De Arias, Francois NoireauAbstract:Background Chagas disease vectors (Hemiptera-Reduviidae) comprise more than 140 blood-sucking insect species of the Triatominae subfamily. The largest genus is Triatoma, subdivided in several complexes and subcomplexes according to morphology, ecology and genetic features. One of them is the sordida subcomplex, involving four species: Triatoma sordida, T. guasayana, T. garciabesi and T. patagonica. Given the great morphological similarity of these species, their taxonomic identification, evolutionary relationships and population differentiation have been controversial for many years and even today remain under discussion.
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New Discoveries of Sylvatic Triatoma infestans (Hemiptera: Reduviidae) Throughout the Bolivian Chaco
American Journal of Tropical Medicine and Hygiene, 2012Co-Authors: Etienne Waleckx, Stéphanie Depickère, Renata Salas, Claudia Aliaga, Marcelo Monje, Hiber Calle, Rosio Buitrago, Francois Noireau, Simone Frederique BreniereAbstract:Sylvatic populations of Triatoma infestans might be involved in the recolonization of human dwellings. We report here the discoveries of new T. infestans sylvatic foci in the Bolivian Chaco. Eighty-one triatomines were caught, 38 of which were identified as T. infestans. Triatoma sordida and Panstrongylus geniculatus were the other species collected. One T. infestans and one T. sordida were infected with Trypanosoma cruzi TcI; one T. infestans was infected with TcII. These discoveries add to the debate on the geographic distribution of sylvatic T. infestans populations, the geographic origin of the species, and the epidemiological role of these populations.
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evolutionary relationships based on genetic and phenetic characters between Triatoma maculata Triatoma pseudomaculata and morphologically related species reduviidae triatominae
Infection Genetics and Evolution, 2007Co-Authors: S Dos M Santos, Francisco Panzera, Catarina Macedo Lopes, Francois Noireau, Jean-pierre Dujardin, Rodrigo Perez, A Carbajal L De La Fuente, Raquel S PachecoAbstract:The maculata group currently comprises two species of Triatominae, Triatoma maculata and Triatoma pseudomaculata, which share morphologic and chromatic characteristics. In order to clarify the systematic status of these two vectors of Trypanosoma cruzi and to infer their evolutionary relationships, we performed an enzymatic, morphometric and cytogenetic comparison of them, also taking into account two sister species not included in the group (T. arthurneivai and T. wygodzinskyi). According to our results, T. maculata and T. pseudomaculata belong to distinct evolutionary lineages. Similarly, T. arthurneivai topotypes from Minas Gerais form an independent isolated group by morphometrics. Our results also support the specific status of the Triatoma population from Sao Paulo State (formerly referred to T. arthurneivai), and suggest the possibility that it is T. wygodzinskyi. Finally, we suggest that only the arboricolous T. pseudomaculata from northeast Brazil and the rupicolous sister species originated from Sao Paulo State should be classified together in the same group.
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flight dispersal of the chagas disease vectors Triatoma brasiliensis and Triatoma pseudomaculata in northeastern brazil
Acta Tropica, 2007Co-Authors: Ana Laura Carbajal De La Fuente, Claudio R Lazzari, Sebastian A Minoli, Catarina Macedo Lopes, Francois Noireau, Marcelo G LorenzoAbstract:The present paper reports for the first time the capture of wild Triatoma brasiliensis and Triatoma pseudomaculata by means of light traps in Brazil. We tested commercially available lighting devices powered by batteries to attract the bugs to a white piece of cloth in the field. Two main findings showed to be significant: first, the results presented here show that light traps can be used for sampling these species in wild environments; second, they reveal that house colonization by triatomines may also happen as a consequence of the arrival of flying sylvatic bugs guided by artificial light sources. In addition, we discuss the effect of some environmental and biological factors on triatomine flight activity modulation.
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Wild Habitat and Related Fauna of Panstrongylus lutzi (Reduviidae, Triatominae)
Journal of Medical Entomology, 2003Co-Authors: Artur G. Dias-lima, Diego Menezes, Ítalo A. Sherlock, Francois NoireauAbstract:Abstract Although Panstrongylus lutzi (Neiva and Pinto) is currently the fifth most frequent species of Triatominae captured in artificial structures in Brazil, its silvatic habitat remains unknown. A survey of its natural ecotopes was performed in an area of Bahia State. P. lutzi nymphal instars and adults were detected in burrows of Dasypodidae. Silvatic habitat of four other endemic triatomine species of the caatinga (Triatoma pseudomaculata Correa and Espinola, Triatoma brasiliensis Neiva, Rhodnius neglectus Lent, and Psammolestes tertius Lent and Jurberg) was also recorded.
Jader De Oliveira - One of the best experts on this subject based on the ideXlab platform.
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characterization of female external genitalia and eggs of four south american species of the Triatoma laporte 1832 genus hemiptera reduviidae triatominae
Insects, 2021Co-Authors: Tiago Belintani, Cleber Galvao, Jader De Oliveira, Juliana Damieli Nascimento, Kaio Cesar Chaboli Alevi, Heloisa Pinotti, Estela Sassocerri, Joao Aristeu Da RosaAbstract:Triatoma is the most diversified and one of the most important genera from an epidemiological perspective. Given the difficulty in identifying some species of the Triatoma genus, morphological, histological, and morphometric studies were performed to provide new characters that make it possible to differentiate T. garciabesi, T. guasayana, T. patagonica, and T. sordida sensu stricto, triatomines that overlap geographically and have vector potential. Through the external female genitalia, as well as morphology, morphometry, and histology of eggshells, it was possible to discriminate the four species. In addition, this study reinforces the taxonomic validity of T. garciabesi and provides new data for discussion on systematic issues of T. guasayana and T. patagonica.
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Trends in taxonomy of Triatomini (Hemiptera, Reduviidae, Triatominae): reproductive compatibility reinforces the synonymization of Meccus Stål, 1859 with Triatoma Laporte, 1832
'Springer Science and Business Media LLC', 2021Co-Authors: Natália Regina Cesaretto, Cleber Galvao, Jader De Oliveira, Fernanda Fernandez Madeira, Amanda Ravazi, Yago Visinho Dos Reis, Ana Beatriz Bortolozo De Oliveira, Roberto Dezan Vicente, Daniel Cesaretto Cristal, Maria Tercília Vilela De Azeredo-oliveiraAbstract:Abstract Background Meccus' taxonomy has been quite complex since the first species of this genus was described by Burmeister in 1835 as Conorhinus phyllosoma. In 1859 the species was transferred to the genus Meccus and in 1930 to Triatoma. However, in the twentieth century, the Meccus genus was revalidated (alteration corroborated by molecular studies) and, in the twenty-first century, through a comprehensive study including more sophisticated phylogenetic reconstruction methods, Meccus was again synonymous with Triatoma. Events of natural hybridization with production of fertile offspring have already been reported among sympatric species of the T. phyllosoma subcomplex, and experimental crosses demonstrated reproductive viability among practically all species of the T. phyllosoma subcomplex that were considered as belonging to the genus Meccus, as well as between these species and species of Triatoma. Based on the above, we carried out experimental crosses between T. longipennis (considered M. longipennis in some literature) and T. mopan (always considered as belonging to Triatoma) to evaluate the reproductive compatibility between species of the T. phyllosoma complex. In addition, we have grouped our results with information from the literature regarding crosses between species that were grouped in the genus Meccus with Triatoma, in order to discuss the importance of experimental crosses to confirm the generic reorganization of species. Results The crosses between T. mopan female and T. longipennis male resulted in viable offspring. The hatching of hybrids, even if only in one direction and/or at low frequency, demonstrates reproductive compatibility and homeology between the genomes of the parents. Conclusion Considering that intergeneric crosses usually do not result in viable offspring in Triatominae, the reproductive compatibility observed between the T. phyllosoma subcomplex species considered in the Meccus genus with species of the Triatoma genus shows that there is “intergeneric” genomic compatibility, which corroborates the generic reorganization of Meccus in Triatoma. Graphic Abstrac
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Formal Assignation of the Kissing Bug Triatoma lecticularia (Hemiptera: Reduviidae: Triatominae) to the Genus ParaTriatoma
'MDPI AG', 2021Co-Authors: Vinicius Fernandes De Paiva, Cleber Galvao, Jader De Oliveira, Silvia Andrade Justi, José Manuel Ayala Landa, Joao Aristeu Da RosaAbstract:The subfamily Triatominae (Hemiptera: Reduviidae) comprises hematophagous insects that are vectors of Chagas disease; including species assigned to the genera Triatoma and ParaTriatoma. Initial examination of Triatoma lecticularia revealed the hirsuteness covering the entire body—a characteristic and striking feature of members of the genus ParaTriatoma—and a systematic study revealed several other morphological characters that are in diagnostic alignment with ParaTriatoma. Based on the examination of several specimens (including the lectotype), and with the additional support of molecular and cytogenetic data, we propose the formal transferal of Triatoma lecticularia (Stål, 1859) into the genus ParaTriatoma with the resulting new combination: ParaTriatoma lecticularia (Stål, 1859) comb. nov. (Hemiptera: Reduviidae: Triatominae)
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molecular cytotaxonomy of the Triatoma brasiliensis species subcomplex hemiptera triatominae
Acta Tropica, 2020Co-Authors: Kaio Cesar Chaboli Alevi, Jader De Oliveira, Joao Aristeu Da Rosa, Isadora De Freitas Bittinelli, Luiza Maria Grzyb Delgado, Fernanda Fernandez Madeira, Mauricio Lilioso, Elaine Follyramos, Maria Tercilia Vilela De AzeredooliveiraAbstract:Abstract The Triatoma genus is paraphyletic, and its species are grouped into complexes and subcomplexes. Given the fact that species that make up a given subcomplex generally share chromosomal traits, we analyzed the distribution of AT- and CG-rich DNA of the T. brasiliensis species subcomplex, in order to establish affinities among members of the T. brasiliensis subcomplex based on chromatin and chromosome traits and develop an identification key for the four monophyletic Triatoma subcomplexes from South America. All species exhibited a CG-rich X sex chromosome and autosomes, as well as an AT-rich Y sex chromosome. This feature can be used as a diagnostic characteristic to determine whether a given species is a member of the T. brasiliensis subcomplex, because it enables the differentiation of these species from all Triatoma of South America. Thus, we confirmed the chromosomal relationship of the T. brasiliensis species subcomplex and developed a dichotomous key based on the chromocenter to differentiate the species from this subcomplex from the other monophyletic Triatoma subcomplexes from South America.
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first report of Triatoma sordida stal 1859 hemiptera reduviidae triatominae in the state of acre and brazilian western amazon
Revista Da Sociedade Brasileira De Medicina Tropical, 2018Co-Authors: Leandro Jose Ramos, Janis Lunier De Souza, Claudio Rodrigues De Souza, Renildo Moura Da Cunha, Jader De Oliveira, Joao Aristeu Da Rosa, Luis Marcelo Aranha Camargo, Dionatas Ulises De Oliveira MeneguettiAbstract:INTRODUCTION: The occurrence of Triatoma sordida in the Brazilian Western Amazon is reported for the first time. METHODS: Triatoma sordida specimens were collected from a Gallus gallus nest in a peridomiciliary area of Senador Guiomard municipality in the state of Acre. RESULTS: The number of triatomine species in Acre increased from six to seven with this first report of T. sordida in the Brazilian Western Amazon. CONCLUSIONS: The occurrence of T. sordida in Acre is of concern since it is among the most captured triatomines in peridomiciliary environments in Brazil, and carries a high potential for vector transmission.
Maria Tercilia Vilela De Azeredooliveira - One of the best experts on this subject based on the ideXlab platform.
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molecular cytotaxonomy of the Triatoma brasiliensis species subcomplex hemiptera triatominae
Acta Tropica, 2020Co-Authors: Kaio Cesar Chaboli Alevi, Jader De Oliveira, Joao Aristeu Da Rosa, Isadora De Freitas Bittinelli, Luiza Maria Grzyb Delgado, Fernanda Fernandez Madeira, Mauricio Lilioso, Elaine Follyramos, Maria Tercilia Vilela De AzeredooliveiraAbstract:Abstract The Triatoma genus is paraphyletic, and its species are grouped into complexes and subcomplexes. Given the fact that species that make up a given subcomplex generally share chromosomal traits, we analyzed the distribution of AT- and CG-rich DNA of the T. brasiliensis species subcomplex, in order to establish affinities among members of the T. brasiliensis subcomplex based on chromatin and chromosome traits and develop an identification key for the four monophyletic Triatoma subcomplexes from South America. All species exhibited a CG-rich X sex chromosome and autosomes, as well as an AT-rich Y sex chromosome. This feature can be used as a diagnostic characteristic to determine whether a given species is a member of the T. brasiliensis subcomplex, because it enables the differentiation of these species from all Triatoma of South America. Thus, we confirmed the chromosomal relationship of the T. brasiliensis species subcomplex and developed a dichotomous key based on the chromocenter to differentiate the species from this subcomplex from the other monophyletic Triatoma subcomplexes from South America.
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chromosomal characteristics and distribution of constitutive heterochromatin in the matogrossensis and rubrovaria subcomplexes
Infection Genetics and Evolution, 2015Co-Authors: Kaio Cesar Chaboli Alevi, Jader De Oliveira, Joao Aristeu Da Rosa, Jose Jurberg, Felipe Ferraz Figueiredo Moreira, Maria Tercilia Vilela De AzeredooliveiraAbstract:Since 1966 the triatomines were grouped in complexes and specific subcomplexes. Although the complex and subcomplexes not have taxonomic importance, should be monophyletic groups and cytogenetic tools have proved to be of great importance to characterize these species groupings. Based on this, this paper aims to describe the chromosomal characteristics and heterochromatic pattern of Matogrossensis and Rubrovaria subcomplexes, in order to contribute to the taxonomic and evolutionary relationships of these vectors. In this study, at least three males from each species (Triatoma baratai, Triatoma costalimai, Triatoma guazu, Triatoma jurbergi, Triatoma matogrossensis, Triatoma vandae, Triatoma williami, Triatoma carcavalloi, Triatoma circummaculata, Triatoma klugi, Triatoma pintodiasi and Triatoma rubrovaria) were analyzed by means analyzed by means of cytogenetic techniques of C-banding. All species showed the same cytogenetic characteristics: 22 chromosomes, low variation in the size of autosomes, sex chromosome Y larger than X, initial prophase composed of only one heterochromatic chromocenter formed by the sex chromosomes X and Y (except for T. pintodiasi that presented the sex chromosomes individualized during all stages of prophase) and presence of constitutive heterochromatin restricted to sex chromosome Y. These characteristics, although common to Matogrossensis and Rubrovaria subcomplexes allow to distinguish these species of species grouped in most of South America subcomplexes, as Brasiliensis, Maculata, Sordida and Insfestans. Thus, the cytogenetic analysis was of extreme importance to differentiate both subcomplexes of the other subcomplexes of South America. However, probably due to evolutionary proximity existing between these subcomplexes was not possible to observar species differences that make up the Matogrossensis subcomplex of the Rubrovaria subcomplex. Therefore, we emphasize that new comparative analyzes, as experimental hybrid crosses and molecular cytogenetic analysis are necessary to clarify the evolutionary relationship between these important subcomplexes of vectors.
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distribution of constitutive heterochromatin in species of triatomines with fragmentation of sex chromosomes x
Genetics and Molecular Research, 2014Co-Authors: Ana Leticia Guerra, Joao Aristeu Da Rosa, Kaio Cesar Chaboli Alevi, Maria Tercilia Vilela De AzeredooliveiraAbstract:Cytogenetic analyses of triatomines are considered to be important taxonomic tools. Thus, we analyzed the pattern of constitutive heterochromatin in 7 species of triatomine with fragmentation of the sex chromosome X, focusing on the cytotaxonomy of these triatomines. The species analyzed included Triatoma vitticeps, Triatoma melanocephala, Triatoma tibiamaculata, Triatoma protracta, Meccus pallidipennis, Panstrongylus megistus, and Panstrongylus lignarius. The seminiferous tubules of the adult males were subjected to C-banding. P. megistus and P. lignarius showed differences in chromosome number and disposition of constitutive heterochromatin, as only P. lignarius showed C-blocks in autosomes. C-banding can differentiate these species, since one of the sex chromosome (X) is heterochromatic in T. vitticeps. T. protracta showed C-blocks in both ends of all autosomes, T. tibiamaculata showed terminal C-dots in some autosomal pairs and M. pallidipennis did not show constitutive heterochromatin in autosomes. Thus, we confirmed the heterochromatic pattern of 7 species of insects and emphasized the importance of cytogenetic techniques for C-banding for taxonomy studies of the triatomines, which are important vectors of Chagas disease.
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spermatogenesis in Triatoma melanocephala hemiptera triatominae
Genetics and Molecular Research, 2013Co-Authors: Kaio Cesar Chaboli Alevi, Joao Aristeu Da Rosa, Priscila Pasquetto Mendonca, Nathalia Paiva Pereira, Maria Tercilia Vilela De AzeredooliveiraAbstract:Triatoma melanocephala is a rare species of Hemiptera. It belonged to the Brasiliensis subcomplex and presents morphological characteristics very close to those of Triatoma vitticeps. We investigated spermatogenesis of T. melanocephala and compared it with that of T. vitticeps in order to determine whether these organisms have similar cytogenetic characteristics. Lacto-acetic orcein staining was used to observe all stages of meiosis. These two species were found to have the same karyotype (2n = 20A + X1X2X3Y), heteropycnotic corpuscles in the polyploid spermatogonial cells, interfasic and profasic nucleus, and chromocenter with four sex chromosomes during prophase. Thus, we conclude that besides the morphologic similarity of T. melanocephala with T. vitticeps, they also have similar spermatogenesis and cytogenetics.
Kaio Cesar Chaboli Alevi - One of the best experts on this subject based on the ideXlab platform.
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characterization of female external genitalia and eggs of four south american species of the Triatoma laporte 1832 genus hemiptera reduviidae triatominae
Insects, 2021Co-Authors: Tiago Belintani, Cleber Galvao, Jader De Oliveira, Juliana Damieli Nascimento, Kaio Cesar Chaboli Alevi, Heloisa Pinotti, Estela Sassocerri, Joao Aristeu Da RosaAbstract:Triatoma is the most diversified and one of the most important genera from an epidemiological perspective. Given the difficulty in identifying some species of the Triatoma genus, morphological, histological, and morphometric studies were performed to provide new characters that make it possible to differentiate T. garciabesi, T. guasayana, T. patagonica, and T. sordida sensu stricto, triatomines that overlap geographically and have vector potential. Through the external female genitalia, as well as morphology, morphometry, and histology of eggshells, it was possible to discriminate the four species. In addition, this study reinforces the taxonomic validity of T. garciabesi and provides new data for discussion on systematic issues of T. guasayana and T. patagonica.
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molecular cytotaxonomy of the Triatoma brasiliensis species subcomplex hemiptera triatominae
Acta Tropica, 2020Co-Authors: Kaio Cesar Chaboli Alevi, Jader De Oliveira, Joao Aristeu Da Rosa, Isadora De Freitas Bittinelli, Luiza Maria Grzyb Delgado, Fernanda Fernandez Madeira, Mauricio Lilioso, Elaine Follyramos, Maria Tercilia Vilela De AzeredooliveiraAbstract:Abstract The Triatoma genus is paraphyletic, and its species are grouped into complexes and subcomplexes. Given the fact that species that make up a given subcomplex generally share chromosomal traits, we analyzed the distribution of AT- and CG-rich DNA of the T. brasiliensis species subcomplex, in order to establish affinities among members of the T. brasiliensis subcomplex based on chromatin and chromosome traits and develop an identification key for the four monophyletic Triatoma subcomplexes from South America. All species exhibited a CG-rich X sex chromosome and autosomes, as well as an AT-rich Y sex chromosome. This feature can be used as a diagnostic characteristic to determine whether a given species is a member of the T. brasiliensis subcomplex, because it enables the differentiation of these species from all Triatoma of South America. Thus, we confirmed the chromosomal relationship of the T. brasiliensis species subcomplex and developed a dichotomous key based on the chromocenter to differentiate the species from this subcomplex from the other monophyletic Triatoma subcomplexes from South America.
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chromosomal characteristics and distribution of constitutive heterochromatin in the matogrossensis and rubrovaria subcomplexes
Infection Genetics and Evolution, 2015Co-Authors: Kaio Cesar Chaboli Alevi, Jader De Oliveira, Joao Aristeu Da Rosa, Jose Jurberg, Felipe Ferraz Figueiredo Moreira, Maria Tercilia Vilela De AzeredooliveiraAbstract:Since 1966 the triatomines were grouped in complexes and specific subcomplexes. Although the complex and subcomplexes not have taxonomic importance, should be monophyletic groups and cytogenetic tools have proved to be of great importance to characterize these species groupings. Based on this, this paper aims to describe the chromosomal characteristics and heterochromatic pattern of Matogrossensis and Rubrovaria subcomplexes, in order to contribute to the taxonomic and evolutionary relationships of these vectors. In this study, at least three males from each species (Triatoma baratai, Triatoma costalimai, Triatoma guazu, Triatoma jurbergi, Triatoma matogrossensis, Triatoma vandae, Triatoma williami, Triatoma carcavalloi, Triatoma circummaculata, Triatoma klugi, Triatoma pintodiasi and Triatoma rubrovaria) were analyzed by means analyzed by means of cytogenetic techniques of C-banding. All species showed the same cytogenetic characteristics: 22 chromosomes, low variation in the size of autosomes, sex chromosome Y larger than X, initial prophase composed of only one heterochromatic chromocenter formed by the sex chromosomes X and Y (except for T. pintodiasi that presented the sex chromosomes individualized during all stages of prophase) and presence of constitutive heterochromatin restricted to sex chromosome Y. These characteristics, although common to Matogrossensis and Rubrovaria subcomplexes allow to distinguish these species of species grouped in most of South America subcomplexes, as Brasiliensis, Maculata, Sordida and Insfestans. Thus, the cytogenetic analysis was of extreme importance to differentiate both subcomplexes of the other subcomplexes of South America. However, probably due to evolutionary proximity existing between these subcomplexes was not possible to observar species differences that make up the Matogrossensis subcomplex of the Rubrovaria subcomplex. Therefore, we emphasize that new comparative analyzes, as experimental hybrid crosses and molecular cytogenetic analysis are necessary to clarify the evolutionary relationship between these important subcomplexes of vectors.
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distribution of constitutive heterochromatin in species of triatomines with fragmentation of sex chromosomes x
Genetics and Molecular Research, 2014Co-Authors: Ana Leticia Guerra, Joao Aristeu Da Rosa, Kaio Cesar Chaboli Alevi, Maria Tercilia Vilela De AzeredooliveiraAbstract:Cytogenetic analyses of triatomines are considered to be important taxonomic tools. Thus, we analyzed the pattern of constitutive heterochromatin in 7 species of triatomine with fragmentation of the sex chromosome X, focusing on the cytotaxonomy of these triatomines. The species analyzed included Triatoma vitticeps, Triatoma melanocephala, Triatoma tibiamaculata, Triatoma protracta, Meccus pallidipennis, Panstrongylus megistus, and Panstrongylus lignarius. The seminiferous tubules of the adult males were subjected to C-banding. P. megistus and P. lignarius showed differences in chromosome number and disposition of constitutive heterochromatin, as only P. lignarius showed C-blocks in autosomes. C-banding can differentiate these species, since one of the sex chromosome (X) is heterochromatic in T. vitticeps. T. protracta showed C-blocks in both ends of all autosomes, T. tibiamaculata showed terminal C-dots in some autosomal pairs and M. pallidipennis did not show constitutive heterochromatin in autosomes. Thus, we confirmed the heterochromatic pattern of 7 species of insects and emphasized the importance of cytogenetic techniques for C-banding for taxonomy studies of the triatomines, which are important vectors of Chagas disease.
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spermatogenesis in Triatoma melanocephala hemiptera triatominae
Genetics and Molecular Research, 2013Co-Authors: Kaio Cesar Chaboli Alevi, Joao Aristeu Da Rosa, Priscila Pasquetto Mendonca, Nathalia Paiva Pereira, Maria Tercilia Vilela De AzeredooliveiraAbstract:Triatoma melanocephala is a rare species of Hemiptera. It belonged to the Brasiliensis subcomplex and presents morphological characteristics very close to those of Triatoma vitticeps. We investigated spermatogenesis of T. melanocephala and compared it with that of T. vitticeps in order to determine whether these organisms have similar cytogenetic characteristics. Lacto-acetic orcein staining was used to observe all stages of meiosis. These two species were found to have the same karyotype (2n = 20A + X1X2X3Y), heteropycnotic corpuscles in the polyploid spermatogonial cells, interfasic and profasic nucleus, and chromocenter with four sex chromosomes during prophase. Thus, we conclude that besides the morphologic similarity of T. melanocephala with T. vitticeps, they also have similar spermatogenesis and cytogenetics.
