Paper Electrophoresis

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The Experts below are selected from a list of 75 Experts worldwide ranked by ideXlab platform

Shashi Agarwal - One of the best experts on this subject based on the ideXlab platform.

J.a. Owen - One of the best experts on this subject based on the ideXlab platform.

  • Paper Electrophoresis of proteins and protein-bound substances in clinical investigations.
    Advances in Clinical Chemistry, 2008
    Co-Authors: J.a. Owen
    Abstract:

    Publisher Summary This chapter describes the role of Paper Electrophoresis of proteins in investigating the chemical nature of physiological and pathological conditions; and the individual patient. The value of Paper Electrophoresis in determining the diagnosis and prognosis, in the assessment of progress, and in the control of therapy has been evaluated in the chapter. Paper Electrophoresis of proteins is a rapidly developing technique, and conclusions drawn at present are scarcely more than those of a preliminary report. It has been very widely used in elucidating the changes in protein metabolism, which occur in different conditions, amplifying and extending the information already obtained by the more laborious technique of boundary Electrophoresis. The use of Paper Electrophoresis as a diagnostic procedure is thus less assured than its use in other fields, and it would appear that techniques that give better resolution of individual protein components, such as Electrophoresis in starch gel or Electrophoresis in agar combined with immunological reactions (G18), could profitably be developed further as aids to diagnosis.

Mary Martinette - One of the best experts on this subject based on the ideXlab platform.

S. Chr. Sommerfelt - One of the best experts on this subject based on the ideXlab platform.

  • Paper Electrophoresis of isolated plasma protein fractions.
    Scandinavian Journal of Clinical & Laboratory Investigation, 2010
    Co-Authors: S. Chr. Sommerfelt
    Abstract:

    AbstractThe purpose of the present investigation has been to examine in more detail a part of the basis for Paper Electrophoresis, namely the relationship between the amount of protein applied and the measurable color intensity. Bovine albumin and γ-globulin, as well as human albumin, α-, β- and γ-globulin were investigated by Grassmann & Hannig's (1952) method, with some modifications (Sommerfelt, to be published). The principle involved is migration on freely suspended, thin Paper, staining with amido-sohwarz 10 B (Bayer) and direct photometry of the strip.1. There is good agreement between the results with the bovine and human preparations.2. Albumin shows the greatest stainability of the fractions investigated, 02-globulin the least.3. During electrorphoretic migration an adsorbtion of protein to the Paper takes place.4. The amount of protein adsorbed is independent of the amount applied, i. e. the Paper becomes saturated and then adsorbs no more of the same protein.5. When the Paper is saturated with...

Dav Girls - One of the best experts on this subject based on the ideXlab platform.