The Experts below are selected from a list of 996 Experts worldwide ranked by ideXlab platform
Gary W. Procop - One of the best experts on this subject based on the ideXlab platform.
-
North American Paragonimiasis (Caused by Paragonimus kellicotti) in the Context of Global Paragonimiasis
Clinical Microbiology Reviews, 2009Co-Authors: Gary W. ProcopAbstract:Summary: Paragonimus species are highly evolved parasites with a complex life cycle that involves at least three different hosts, i.e., snails, crustaceans, and mammals. The adult forms of Paragonimus species reside and mate in the lungs of a variety of permissive mammalian hosts, including humans. Although human Paragonimiasis is uncommonly encountered in North America, both autochthonous and imported disease may be encountered. Paragonimus kellicotti, the species endemic to North America, is a well-known pathogen in wild and domestic animals. Five patients with North American Paragonimiasis have been reported in the recent medical literature. The biologic, clinical, radiologic, and laboratory features of Paragonimiasis are reviewed, with emphasis on North American Paragonimiasis whenever possible.
-
Cavitary mass lesion and recurrent pneumothoraces due to Paragonimus kellicotti infection: North American Paragonimiasis.
The American Journal of Surgical Pathology, 2003Co-Authors: Elias A. Castilla, Robert Jessen, David N. Sheck, Gary W. ProcopAbstract:North American Paragonimiasis is well described in omnivorous and carnivorous animals on this continent. Humans are rarely infected, largely because of dietary customs, but are at risk for infection if raw or undercooked crayfish are consumed. We describe a patient with a pleuropulmonary infection due to Paragonimus kellicotti that presented as recurrent pneumothoraces and a cavitary lesion. This is the first case of North American Paragonimiasis in which the diagnosis was based on the morphology of the eggs present in histologic sections.
Sung-jong Hong - One of the best experts on this subject based on the ideXlab platform.
-
CONCISE COMMUNICATION Recombinant Paragonimus westermani Yolk Ferritin Is a Useful Serodiagnostic Antigen
2016Co-Authors: Sung-jong HongAbstract:A recombinant protein of Paragonimus westermani yolk ferritin was bacterially produced from a previously cloned complementary DNA and was used as an antigen for an enzyme-linked immu-nosorbent assay (ELISA) against Paragonimiasis- and other helminth-infected sera to evaluate its serodiagnostic potential. The ELISA revealed that Paragonimiasis westermani had 88.2 % sensitivity and 100 % specificity. The positive and negative predictive values of the ELISA were calculated to be 100 % and 97.1%, respectively. Sera from cats experimentally infected with P. westermani began to produce immunoglobulin G antibodies against the yolk ferritin at 13 weeks after infec-tion, which suggests that the corresponding antigen was derived from the vitellaria in accordance with maturation of P. westermani. These results indicate that the recombinant P. westermani yolk ferritin is a potent serodiagnostic reagent for Paragonimiasis westermani from an early stage of the infection. Paragonimiasis is a disease caused by lung flukes of genus Para-gonimus helminths. Pulmonary Paragonimiasis, caused by P. west-ermani infection, is endemic in the Far East, southeastern Asia, and South America and remains a serious public health proble
-
Antigenicity of recombinant C. sinensis growth factor receptor-bound protein towards IgG antibody in C. sinensis and helminth-infected human sera by ELISA.
2014Co-Authors: Xuelian Bai, Ji-yun Lee, Tae Im Kim, Fuhong Dai, Tae-jin Lee, Sung-jong HongAbstract:Cs, clonorchiasis sinensis; Pw, Paragonimiasis westermani; Cy, cysticercosis cellulosae; Sj, schistosomiasis japonicum; Normal, uninfected human sera were used as a negative control.
-
recombinant paragonimus westermani yolk ferritin is a useful serodiagnostic antigen
The Journal of Infectious Diseases, 2002Co-Authors: Shinyong Kang, Yoon Kong, Kom Sukomtason, Sung-jong HongAbstract:A recombinant protein of Paragonimus westermani yolk ferritin was bacterially produced from a previously cloned complementary DNA and was used as an antigen for an enzyme-linked immunosorbent assay (ELISA) against Paragonimiasis- and other helminth-infected sera to evaluate its serodiagnostic potential. The ELISA revealed that Paragonimiasis westermani had 88.2% sensitivity and 100% specificity. The positive and negative predictive values of the ELISA were calculated to be 100% and 97.1%, respectively. Sera from cats experimentally infected with P. westermani began to produce immunoglobulin G antibodies against the yolk ferritin at 13 weeks after infection, which suggests that the corresponding antigen was derived from the vitellaria in accordance with maturation of P. westermani. These results indicate that the recombinant P. westermani yolk ferritin is a potent serodiagnostic reagent for Paragonimiasis westermani from an early stage of the infection.
Yukifumi Nawa - One of the best experts on this subject based on the ideXlab platform.
-
elevated levels of thymus and activation regulated chemokine tarc in pleural effusion samples from patients infested with paragonimus westermani
Clinical and Experimental Immunology, 2002Co-Authors: N Matsumoto, Jun-ichi Ashitani, Hiroshi Mukae, Yukifumi Nawa, Fukumi Nakamurauchiyama, Shigeki Katoh, Shigeru Kohno, Kuniko Abe, Shigeru MatsukuraAbstract:Summary To investigate the pathogenic mechanisms of eosinophilic pleural effusion in patients with Paragonimiasis, we measured the levels of various chemokines including thymus and activation-regulated chemokine (TARC), eotaxin, RANTES and IL-8 in pleural effusion samples. Samples were obtained from 11 patients with Paragonimus westermani infection, six patients with pleural transudate, eight with tuberculous pleurisy and five with empyema. High percentages of eosinophils were detected in pleural fluid (range 9–100%, median 81%) of patients with Paragonimiasis. TARC concentrations in pleural effusions of Paragonimiasis were markedly higher than those of other groups. Eotaxin levels were also higher in pleural effusions of Paragonimiasis patients, although significant difference was noted only against transudate samples. There was a significant correlation between TARC concentrations and percentages of eosinophils, and between TARC and eotaxin concentrations in pleural effusion. There were also significant correlations between TARC concentration and the titre of anti-P. westermani IgG and between eotaxin concentration and the titre of anti-P. westermani IgG. Our findings suggest that TARC contributes to the pathogenesis of eosinophilic pleural effusion in Paragonimiasis.
-
elevated levels of thymus and activation regulated chemokine tarc in pleural effusion samples from patients infested with paragonimus westermani
Clinical and Experimental Immunology, 2002Co-Authors: Nobuhiro Matsumoto, Jun-ichi Ashitani, Hiroshi Mukae, Yukifumi Nawa, Fukumi Nakamurauchiyama, Shigeki Katoh, Shigeru Kohno, Shigeru MatsukuraAbstract:Summary To investigate the pathogenic mechanisms of eosinophilic pleural effusion in patients with Paragonimiasis, we measured the levels of various chemokines including thymus and activation-regulated chemokine (TARC), eotaxin, RANTES and IL-8 in pleural effusion samples. Samples were obtained from 11 patients with Paragonimus westermani infection, six patients with pleural transudate, eight with tuberculous pleurisy and five with empyema. High percentages of eosinophils were detected in pleural fluid (range 9–100%, median 81%) of patients with Paragonimiasis. TARC concentrations in pleural effusions of Paragonimiasis were markedly higher than those of other groups. Eotaxin levels were also higher in pleural effusions of Paragonimiasis patients, although significant difference was noted only against transudate samples. There was a significant correlation between TARC concentrations and percentages of eosinophils, and between TARC and eotaxin concentrations in pleural effusion. There were also significant correlations between TARC concentration and the titre of anti-P. westermani IgG and between eotaxin concentration and the titre of anti-P. westermani IgG. Our findings suggest that TARC contributes to the pathogenesis of eosinophilic pleural effusion in Paragonimiasis.
-
clinicoradiologic features of pleuropulmonary paragonimus westermani on kyusyu island japan
Chest, 2001Co-Authors: Hiroshi Mukae, Haruko Taniguchi, Hirotoshi Iiboshi, Jun-ichi Ashitani, Shigeru Matsukura, Nobuhiro Matsumoto, Yukifumi NawaAbstract:Study objective Recently, the number of new cases of Paragonimus westermani in humans has gradually increased, and Paragonimiasis is a re-emerging public health issue in Kyusyu, Japan. We review our recent experience with pleuropulmonary Paragonimus westermani . Patients Pulmonary Paragonimiasis was diagnosed in 13 patients at the Third Department of Internal Medicine, Miyazaki Medical College between 1993 and 1999. Results Both sputum and bronchoscopic examinations revealed ova in four of nine patients; bronchoscopy yielded ova in two additional patients. Twelve patients (92%) had respiratory symptoms, including cough (92%), sputum and/or hemoptysis (92%), and chest pain (46%). Chest radiography and CT showed pleural lesions (62%) and parenchymal lesions (92%). Of note was the high frequency of solitary nodular lesions (62%), mimicking lung cancer, tuberculosis, or fungal diseases. Immunodiagnosis and bronchoscopic examination were also useful for diagnosis. Praziquantel treatment was very effective and had minimal side effects. One patient required surgical decortication for empyema in spite of treatment with praziquantel. Eosinophilia was noted in peripheral blood and body fluids, which was probably due to increased levels of interleukin-5. Conclusions Our findings indicate that our patients with Paragonimus westermani presented with a wide variety of radiographic findings, which were different from the classic presentations reported earlier. Bronchoscopic examination and serologic tests are very useful for accurate diagnosis. As dietary habits change and international transportation increases, it appears likely that Paragonimiasis will also increase in frequency in various parts of the world.
-
clinical features of Paragonimiasis cases recently found in japan parasite specific immunoglobulin m and g antibody classes
Clinical Infectious Diseases, 2001Co-Authors: Fukumi Nakamurauchiyam, N Onah, Yukifumi NawaAbstract:We retrospectively analyzed clinical features in 30 patients who were referred to our laboratory and given a diagnosis of Paragonimus westermani infection in 1999. Our results indicate that pleurisy with eosinophilia and dominant immunoglobulin (Ig) M antibody are characteristic features of the early stage of Paragonimiasis, whereas IgG antibody is dominant in the late stage. Thus, in addition to tests for parasite-specific IgG antibody, tests for IgM-class antibody should always be considered for patients with pleurisy in whom Paragonimiasis is suspected.
Kom Sukomtason - One of the best experts on this subject based on the ideXlab platform.
-
recombinant paragonimus westermani yolk ferritin is a useful serodiagnostic antigen
The Journal of Infectious Diseases, 2002Co-Authors: Shinyong Kang, Yoon Kong, Kom Sukomtason, Sung-jong HongAbstract:A recombinant protein of Paragonimus westermani yolk ferritin was bacterially produced from a previously cloned complementary DNA and was used as an antigen for an enzyme-linked immunosorbent assay (ELISA) against Paragonimiasis- and other helminth-infected sera to evaluate its serodiagnostic potential. The ELISA revealed that Paragonimiasis westermani had 88.2% sensitivity and 100% specificity. The positive and negative predictive values of the ELISA were calculated to be 100% and 97.1%, respectively. Sera from cats experimentally infected with P. westermani began to produce immunoglobulin G antibodies against the yolk ferritin at 13 weeks after infection, which suggests that the corresponding antigen was derived from the vitellaria in accordance with maturation of P. westermani. These results indicate that the recombinant P. westermani yolk ferritin is a potent serodiagnostic reagent for Paragonimiasis westermani from an early stage of the infection.
Luis A. Marcos - One of the best experts on this subject based on the ideXlab platform.
-
Serological Diagnosis of North American Paragonimiasis by Western Blot Using Paragonimus kellicotti Adult Worm Antigen
2013Co-Authors: Peter U. Fischer, Kurt C. Curtis, Scott M. Folk, Patricia P. Wilkins, Luis A. MarcosAbstract:Serological diagnosis of North American Paragonimiasis by western blot using Paragonimus kellicotti adult worm antige
-
Paragonimus kellicotti Flukes in Missouri, USA
Centers for Disease Control and Prevention, 2012Co-Authors: Michael A. Lane, Luis A. Marcos, Nur F. Önen, Lee M. Demertzis, Samuel Z. Davila, Diana Nurutdinova, Ericka V. Hayes, Thomas C. BaileyAbstract:Paragonimiasis is an infection caused by lung flukes of the genus Paragonimus. In Asia, P. westermani infections are relatively common because of dietary practices. However, in North America, cases of Paragonimiasis, which are caused by P. kellicotti flukes, are rare. Only 7 autochthonous cases of Paragonimiasis were reported during 1968–2008. In 2009, we reported 3 new case-patients with Paragonimiasis who had been seen at our medical center over an 18-month period. Six additional case-patients were identified in St. Louis, Missouri, USA, and treated at Washington University–affiliated health centers in 2009–2010. We report detailed descriptions of these case-patients, which includes unusual clinical manifestations. We also describe public health interventions that were undertaken to inform the general public and physicians about the disease and its mode of transmission
-
Molecular Characterization of the North American Lung Fluke Paragonimus kellicotti in Missouri and its Development in Mongolian Gerbils
The American Journal of Tropical Medicine and Hygiene, 2011Co-Authors: Peter U. Fischer, Kurt C. Curtis, Luis A. MarcosAbstract:1This food-borne trematode infection is caused by Paragonimus species; humans become infected when they consume raw or undercooked crab or crayfish meat that contains infective parasite larvae (metacercariae). Most human infections occur in the Far East (e.g., P. westermani, P. skriabini ), but human Paragonimiasis also occurs in sub-Saharan Africa ( P. uterobilateralis, P. africanus ) and in the Americas ( P. mexicanus, P. kellicotti ). The parasites migrate from the intestine across the diaphragm to the lungs; clinical symptoms often include cough, fever, weight loss, pleural effusions, and (sometimes) bloody sputum. Increased eosinophils in blood and pleural fluid help to differentiate Paragonimiasis from tuberculosis. Paragonimiasis is easily cured with a two day course of the oral medication praziquantel. North American Paragonimiasis is caused by P. kellicotti , which is widely distributed in small carnivores such as mink, skunks, otters, and other mammals that feed on crayfish. Adult parasites have a life expectancy of several years, and asymptomatic infections in animals are common. Human infections are rare in North America, with only seven cases published before 2009. 2, 3 However, autochthonous Paragonimiasis has recently emerged as a locally important problem in Missouri; 11 cases have been reported since 2006. All of these patients reported that they had eaten raw crayfish during camping or recreational floating trips on Missouri Ozarks streams prior to the onset of their illness. 2, 4
