The Experts below are selected from a list of 276 Experts worldwide ranked by ideXlab platform

Marair Gracio Ferreira Sartori - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of glycosaminoglycans in the Parametrium and vaginal apex of women with and without uterine prolapse.
    Journal of women's health (2002), 2010
    Co-Authors: Lea Mina Kati, Marair Gracio Ferreira Sartori, Helena B. Nader, Paulo Cezar Feldner, Rodrigo Aquino De Castro, Elsa Yoko Kobayashi, Manoel João Batista Castello Girão
    Abstract:

    Abstract Background: Pelvic organ prolapse (POP) is a downward descent of pelvic organs that results in protrusions of the vagina, the uterus, or both. The cause of this disorder is likely to be multifactorial, attributable to a combination of risk factors, especially connective tissue disorders. Our objective was to characterize and quantify a component of the extracellular matrix (ECM)—sulfated glycosaminoglycan (GAG)—in the Parametrium and vaginal apex of women with and without uterine prolapse. Methods: Parametrium and vaginal apex tissue was obtained from 42 women who underwent surgery. Patients underwent a physical examination and were divided into groups according to the type of genital prolapse. Standard biopsies were taken during surgery and were assessed by biochemical methods. GAGs were obtained by proteolysis. The relative concentration of GAGs was determined by densitometry. Data were compared using an independent sample t-test or χ2 test. Results: In both groups (with and without prolapse) a...

  • Analysis of type I collagen in the Parametrium of women with and without uterine prolapse, according to hormonal status.
    International urogynecology journal and pelvic floor dysfunction, 2003
    Co-Authors: Eliana Cristina Barbiero, Marair Gracio Ferreira Sartori, Edmund Chada Baracat, Manoel João Batista Castello Girão, Geraldo Rodrigues De Lima
    Abstract:

    The content and quality of type I collagen in the Parametrium of women with and without uterine prolapse was evaluated. Forty-four consecutive patients were selected and divided into two groups: A, 21 women without uterine prolapse, and B, 23 with uterine prolapse. Patients in group A had uterine leiomyoma and were submitted to abdominal hysterectomy; in those from group B, vaginal hysterectomy was performed for correction of the uterine prolapse. During surgery, fragments of the Parametrium were removed and processed for immunohistochemical analysis using polyclonal antibodies for type I collagen . A system of computerized digital imaging analysis was used for the quantification of collagen fibers. There was no difference between collagen content in patients either with or without prolapse, nor between pre- and postmenopausal women with prolapse. A modification of the quality of the collagen fiber was observed, it being longer and more compact in the group without uterine prolapse. In contrast, in the group with prolapse, the fibers were shorter and thinner and areas with large spaces between fibers were found at several points of the Parametrium. The conclusion was that patients with uterine prolapse have the same type I collagen content as those without, but the quality of the fiber is modified. The hormonal status also did not affect collagen content.

  • analysis of collagen in Parametrium and vaginal apex of women with and without uterine prolapse
    International Urogynecology Journal, 2002
    Co-Authors: Claudia Cristina Takano, Marair Gracio Ferreira Sartori, Rodrigo De Aquino Castro, Raquel Martins Arruda, M Simo J Es, Edmund Chada Baracat, Rodrigues G De Lima
    Abstract:

    Our objective was to compare the amount of collagen in Parametrium and vaginal apex between women with uterine prolapse at pre- and postmenopause, and in women without prolapse. The study included 22 premenopausal women without prolapse (group A), 10 premenopausal women with prolapse (group B), and 23 postmenopausal women with prolapse (group C) (total 55). Patients in group A underwent abdominal hysterectomy for uterine leiomyoma, and patients in groups B and C underwent vaginal hysterectomy. During the surgical procedure we obtained biopsies from the lateral Parametrium and vaginal apex. The tissue was stained for histological analysis with picrosirius. We observed a lower amount of collagen in the Parametrium of women with uterine prolapse, both in menacme and in postmenopause, than in the Parametrium of women without prolapse. We observed no statistically significant difference in vaginal apex between the groups.

Manoel João Batista Castello Girão - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of glycosaminoglycans in the Parametrium and vaginal apex of women with and without uterine prolapse.
    Journal of women's health (2002), 2010
    Co-Authors: Lea Mina Kati, Marair Gracio Ferreira Sartori, Helena B. Nader, Paulo Cezar Feldner, Rodrigo Aquino De Castro, Elsa Yoko Kobayashi, Manoel João Batista Castello Girão
    Abstract:

    Abstract Background: Pelvic organ prolapse (POP) is a downward descent of pelvic organs that results in protrusions of the vagina, the uterus, or both. The cause of this disorder is likely to be multifactorial, attributable to a combination of risk factors, especially connective tissue disorders. Our objective was to characterize and quantify a component of the extracellular matrix (ECM)—sulfated glycosaminoglycan (GAG)—in the Parametrium and vaginal apex of women with and without uterine prolapse. Methods: Parametrium and vaginal apex tissue was obtained from 42 women who underwent surgery. Patients underwent a physical examination and were divided into groups according to the type of genital prolapse. Standard biopsies were taken during surgery and were assessed by biochemical methods. GAGs were obtained by proteolysis. The relative concentration of GAGs was determined by densitometry. Data were compared using an independent sample t-test or χ2 test. Results: In both groups (with and without prolapse) a...

  • Analysis of type I collagen in the Parametrium of women with and without uterine prolapse, according to hormonal status.
    International urogynecology journal and pelvic floor dysfunction, 2003
    Co-Authors: Eliana Cristina Barbiero, Marair Gracio Ferreira Sartori, Edmund Chada Baracat, Manoel João Batista Castello Girão, Geraldo Rodrigues De Lima
    Abstract:

    The content and quality of type I collagen in the Parametrium of women with and without uterine prolapse was evaluated. Forty-four consecutive patients were selected and divided into two groups: A, 21 women without uterine prolapse, and B, 23 with uterine prolapse. Patients in group A had uterine leiomyoma and were submitted to abdominal hysterectomy; in those from group B, vaginal hysterectomy was performed for correction of the uterine prolapse. During surgery, fragments of the Parametrium were removed and processed for immunohistochemical analysis using polyclonal antibodies for type I collagen . A system of computerized digital imaging analysis was used for the quantification of collagen fibers. There was no difference between collagen content in patients either with or without prolapse, nor between pre- and postmenopausal women with prolapse. A modification of the quality of the collagen fiber was observed, it being longer and more compact in the group without uterine prolapse. In contrast, in the group with prolapse, the fibers were shorter and thinner and areas with large spaces between fibers were found at several points of the Parametrium. The conclusion was that patients with uterine prolapse have the same type I collagen content as those without, but the quality of the fiber is modified. The hormonal status also did not affect collagen content.

Rodrigues G De Lima - One of the best experts on this subject based on the ideXlab platform.

  • analysis of collagen in Parametrium and vaginal apex of women with and without uterine prolapse
    International Urogynecology Journal, 2002
    Co-Authors: Claudia Cristina Takano, Marair Gracio Ferreira Sartori, Rodrigo De Aquino Castro, Raquel Martins Arruda, M Simo J Es, Edmund Chada Baracat, Rodrigues G De Lima
    Abstract:

    Our objective was to compare the amount of collagen in Parametrium and vaginal apex between women with uterine prolapse at pre- and postmenopause, and in women without prolapse. The study included 22 premenopausal women without prolapse (group A), 10 premenopausal women with prolapse (group B), and 23 postmenopausal women with prolapse (group C) (total 55). Patients in group A underwent abdominal hysterectomy for uterine leiomyoma, and patients in groups B and C underwent vaginal hysterectomy. During the surgical procedure we obtained biopsies from the lateral Parametrium and vaginal apex. The tissue was stained for histological analysis with picrosirius. We observed a lower amount of collagen in the Parametrium of women with uterine prolapse, both in menacme and in postmenopause, than in the Parametrium of women without prolapse. We observed no statistically significant difference in vaginal apex between the groups.

Edmund Chada Baracat - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of type I collagen in the Parametrium of women with and without uterine prolapse, according to hormonal status.
    International urogynecology journal and pelvic floor dysfunction, 2003
    Co-Authors: Eliana Cristina Barbiero, Marair Gracio Ferreira Sartori, Edmund Chada Baracat, Manoel João Batista Castello Girão, Geraldo Rodrigues De Lima
    Abstract:

    The content and quality of type I collagen in the Parametrium of women with and without uterine prolapse was evaluated. Forty-four consecutive patients were selected and divided into two groups: A, 21 women without uterine prolapse, and B, 23 with uterine prolapse. Patients in group A had uterine leiomyoma and were submitted to abdominal hysterectomy; in those from group B, vaginal hysterectomy was performed for correction of the uterine prolapse. During surgery, fragments of the Parametrium were removed and processed for immunohistochemical analysis using polyclonal antibodies for type I collagen . A system of computerized digital imaging analysis was used for the quantification of collagen fibers. There was no difference between collagen content in patients either with or without prolapse, nor between pre- and postmenopausal women with prolapse. A modification of the quality of the collagen fiber was observed, it being longer and more compact in the group without uterine prolapse. In contrast, in the group with prolapse, the fibers were shorter and thinner and areas with large spaces between fibers were found at several points of the Parametrium. The conclusion was that patients with uterine prolapse have the same type I collagen content as those without, but the quality of the fiber is modified. The hormonal status also did not affect collagen content.

  • analysis of collagen in Parametrium and vaginal apex of women with and without uterine prolapse
    International Urogynecology Journal, 2002
    Co-Authors: Claudia Cristina Takano, Marair Gracio Ferreira Sartori, Rodrigo De Aquino Castro, Raquel Martins Arruda, M Simo J Es, Edmund Chada Baracat, Rodrigues G De Lima
    Abstract:

    Our objective was to compare the amount of collagen in Parametrium and vaginal apex between women with uterine prolapse at pre- and postmenopause, and in women without prolapse. The study included 22 premenopausal women without prolapse (group A), 10 premenopausal women with prolapse (group B), and 23 postmenopausal women with prolapse (group C) (total 55). Patients in group A underwent abdominal hysterectomy for uterine leiomyoma, and patients in groups B and C underwent vaginal hysterectomy. During the surgical procedure we obtained biopsies from the lateral Parametrium and vaginal apex. The tissue was stained for histological analysis with picrosirius. We observed a lower amount of collagen in the Parametrium of women with uterine prolapse, both in menacme and in postmenopause, than in the Parametrium of women without prolapse. We observed no statistically significant difference in vaginal apex between the groups.

Geraldo Rodrigues De Lima - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of type I collagen in the Parametrium of women with and without uterine prolapse, according to hormonal status.
    International urogynecology journal and pelvic floor dysfunction, 2003
    Co-Authors: Eliana Cristina Barbiero, Marair Gracio Ferreira Sartori, Edmund Chada Baracat, Manoel João Batista Castello Girão, Geraldo Rodrigues De Lima
    Abstract:

    The content and quality of type I collagen in the Parametrium of women with and without uterine prolapse was evaluated. Forty-four consecutive patients were selected and divided into two groups: A, 21 women without uterine prolapse, and B, 23 with uterine prolapse. Patients in group A had uterine leiomyoma and were submitted to abdominal hysterectomy; in those from group B, vaginal hysterectomy was performed for correction of the uterine prolapse. During surgery, fragments of the Parametrium were removed and processed for immunohistochemical analysis using polyclonal antibodies for type I collagen . A system of computerized digital imaging analysis was used for the quantification of collagen fibers. There was no difference between collagen content in patients either with or without prolapse, nor between pre- and postmenopausal women with prolapse. A modification of the quality of the collagen fiber was observed, it being longer and more compact in the group without uterine prolapse. In contrast, in the group with prolapse, the fibers were shorter and thinner and areas with large spaces between fibers were found at several points of the Parametrium. The conclusion was that patients with uterine prolapse have the same type I collagen content as those without, but the quality of the fiber is modified. The hormonal status also did not affect collagen content.