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Anchalee Tassanakajon - One of the best experts on this subject based on the ideXlab platform.

  • Regulation of antilipopolysaccharide factors, ALFPm3 and ALFPm6, in Penaeus monodon
    Nature Publishing Group, 2017
    Co-Authors: Pitchayanan Kamsaeng, Anchalee Tassanakajon, Kunlaya Somboonwiwat
    Abstract:

    Abstract ALFPm6, a member of antimicrobial peptide in the antilipopolysaccharide factor (ALF) family from Penaeus monodon, plays important roles in shrimp immunity against pathogens. However, its antimicrobial activity and underlying mechanism have not been reported. The synthetic cyclic ALFPm6#29–52 peptide (cALFPm6#29–52) corresponding to the ALFPm6 LPS-binding domain can agglutinate and exhibited bacterial killing activity toward a Gram-negative bacterium, Escherichia coli 363 and Gram-positive bacteria, Bacillus megaterium, Aerococcus viridans, and Micrococcus luteus, with MIC values of 25–50 μM. Specifically, ALFPm6 and ALFPm3, the most abundant ALF isoforms, are different in terms of gene expression patterns upon pathogen infections. Herein, the regulation of ALFPm3 and ALFPm6 gene expression was studied. The 5′-upstream and promoter sequences were identified and the putative transcription factor (TF)-binding sites were predicted. The narrow down assay indicated that the ALFPm3 promoter and partial promoter of the ALFPm6 active regions were located at nucleotide positions (−814/+302) and (−282/+85), respectively. Mutagenesis of selected TF-binding sites revealed that Rel/NF-κB (−280/−270) of ALFPm3 and C/EBPβ (−88/−78) and Sp1 (−249/−238) sites of ALFPm6 were the activator-binding sites. Knockdown of the PmMyD88 and PmRelish genes in V. harveyi-infected shrimp suggested that the ALFPm3 gene was regulated by Toll and IMD pathways, while the ALFPm6 gene was regulated by the Toll pathway

  • differential gene expression in black tiger shrimp Penaeus monodon following administration of oxytetracycline and oxolinic acid
    Developmental and Comparative Immunology, 2009
    Co-Authors: Fernand F Fagutao, Hidehiro Kondo, Anchalee Tassanakajon, Motoshige Yasuike, Mudjekeewis D Santos, Lila Ruangpan, Kulvara Sangrunggruang, Yuikinori Takahashi, Ryuji Ueno, Ikuo Hirono
    Abstract:

    The intensification of shrimp farming systems has led to the spreading of a variety of bacterial and viral diseases that continue to plague the shrimp industry worldwide. Efforts to combat these pathogenic organisms include the use of immunostimulants, probiotics, vaccines and antibiotics. Although a few studies have already reported on the effects of various stimuli on shrimp, the effect of antibiotics, particularly on the changes in the shrimp transcriptomic profile have yet to be reported. Here we show that injecting shrimp with oxytetracycline and oxolinic acid alters the expression of genes in the black tiger shrimp, Penaeus monodon, lymphoid organ. These antibiotics, especially oxylinic acid, downregulated the expression of a few immune-related genes, most notably penaeidin, proPO, clotting protein, profilin and whey acidic protein.

  • Penaeus monodon gene discovery project the generation of an est collection and establishment of a database
    Gene, 2006
    Co-Authors: Anchalee Tassanakajon, Kallaya Sritunyalucksana, Sirawut Klinbunga, Narongsak Paunglarp, Vichien Rimphanitchayakit, Apinun Udomkit, Sarawut Jitrapakdee
    Abstract:

    Abstract A large-scale expressed sequence tag (EST) sequencing project was undertaken for the purpose of gene discovery in the black tiger shrimp Penaeus monodon. Initially, 15 cDNA libraries were constructed from different tissues (eyestalk, hepatopancrease, haematopoietic tissue, haemocyte, lymphoid organ, and ovary) of shrimp, reared under normal or stress conditions, to identify tissue-specific genes and genes responding to infection and heat stress. A total of 10,100 clones were analyzed by single-pass sequencing from the 5′ end. Clustering and assembling of these ESTs resulted in a total of 4845 unique sequences with 917 overlapping contigs and 3928 singletons. The redundancy of each cDNA library ranged from 13.4% to 61.3% with an overall redundancy of 61.1%. About half of these ESTs (2365 clones, 48.8%) showed significant homology (BLASTX, e-values http://pmodon.biotec.or.th ). This EST database provides a useful resource for gene identification and functional genomic studies of shrimp.

  • Penaeus monodon gene discovery project the generation of an est collection and establishment of a database
    Gene, 2006
    Co-Authors: Anchalee Tassanakajon, Kallaya Sritunyalucksana, Sirawut Klinbunga, Narongsak Paunglarp, Vichien Rimphanitchayakit, Apinun Udomkit, Sarawut Jitrapakdee
    Abstract:

    Abstract A large-scale expressed sequence tag (EST) sequencing project was undertaken for the purpose of gene discovery in the black tiger shrimp Penaeus monodon. Initially, 15 cDNA libraries were constructed from different tissues (eyestalk, hepatopancrease, haematopoietic tissue, haemocyte, lymphoid organ, and ovary) of shrimp, reared under normal or stress conditions, to identify tissue-specific genes and genes responding to infection and heat stress. A total of 10,100 clones were analyzed by single-pass sequencing from the 5′ end. Clustering and assembling of these ESTs resulted in a total of 4845 unique sequences with 917 overlapping contigs and 3928 singletons. The redundancy of each cDNA library ranged from 13.4% to 61.3% with an overall redundancy of 61.1%. About half of these ESTs (2365 clones, 48.8%) showed significant homology (BLASTX, e-values http://pmodon.biotec.or.th ). This EST database provides a useful resource for gene identification and functional genomic studies of shrimp.

  • identification of immune related genes in hemocytes of black tiger shrimp Penaeus monodon
    Marine Biotechnology, 2002
    Co-Authors: Premruethai Supungul, Ikuo Hirono, Takashi Aoki, Sirawut Klinbunga, Sarawut Jitrapakdee, Rath Pichyangkura, Anchalee Tassanakajon
    Abstract:

    An expressed sequence tag (EST) library was constructed from hemocytes of the black tiger shrimp (Penaeus monodon) to identify genes associated with immunity in this economically important species. The number of complementary DNA clones in the constructed library was approximately 4 x 10(5). Of these, 615 clones having inserts larger than 500 bp were unidirectionally sequenced and analyzed by homology searches against data in GenBank. Significant homology to known genes was found in 314 (51%) of the 615 clones, but the remaining 301 sequences (49%) did not match any sequence in GenBank. Approximately 35% of the matched ESTs were significantly identified by the BLASTN and BLASTX programs, while 65% were recognized only by the BLASTX program. Of the 615 clones, 55 (8.9%) were identified as putative immune-related genes. The isolated genes were composed of those coding for enzymes and proteins in the clotting system and the prophenoloxidase-activating system, antioxidative enzymes, antimicrobial peptides, and serine proteinase inhibitors. Three full-length ESTs encoding antimicrobial peptides (antilipopolysaccharide and penaeidin homologues) and a heat shock protein (cpn10 homologue) are reported.

Ikuo Hirono - One of the best experts on this subject based on the ideXlab platform.

  • Successful yellow head virus infection of Penaeus monodon requires clathrin heavy chain.
    Aquaculture (Amsterdam Netherlands), 2014
    Co-Authors: Pratsaneeyaporn Posiri, Ikuo Hirono, Sakol Panyim, Hidehiro Kondo, Chalermporn Ongvarrasopone
    Abstract:

    Viral disease caused by the Yellow head virus (YHV) had great impact on economic loss in the aquaculture industry. Prevention or curing YHV disease is still not possible due to the lack of understanding of the basic mechanisms of YHV infection. In this report, the endocytosis inhibitors (chlorpromazine (CPZ), amiloride and methyl-β-cyclodextrin (MβCD)) were used to identify the cellular entry pathway of YHV. Pretreating shrimp with CPZ but not amiloride or MβCD followed by YHV challenge resulted in a significant reduction of YHV levels, suggesting that YHV entered the shrimp cells via clathrin-mediated endocytosis. Next, the major component of the clathrin-coated vesicle, Penaeus monodon clathrin heavy chain (PmCHC) was cloned and characterized. The complete coding sequence of PmCHC is 5055 bp encoding a putative protein of 1684 amino acids. Specific silencing of PmCHC mRNA by dsRNA-PmCHC showed an inhibition of YHV replication for 48 h post YHV injection as well as exhibiting a delay in shrimp mortality. These results indicated that PmCHC was an essential component for YHV infection of shrimp cells.

  • differential gene expression in black tiger shrimp Penaeus monodon following administration of oxytetracycline and oxolinic acid
    Developmental and Comparative Immunology, 2009
    Co-Authors: Fernand F Fagutao, Hidehiro Kondo, Anchalee Tassanakajon, Motoshige Yasuike, Mudjekeewis D Santos, Lila Ruangpan, Kulvara Sangrunggruang, Yuikinori Takahashi, Ryuji Ueno, Ikuo Hirono
    Abstract:

    The intensification of shrimp farming systems has led to the spreading of a variety of bacterial and viral diseases that continue to plague the shrimp industry worldwide. Efforts to combat these pathogenic organisms include the use of immunostimulants, probiotics, vaccines and antibiotics. Although a few studies have already reported on the effects of various stimuli on shrimp, the effect of antibiotics, particularly on the changes in the shrimp transcriptomic profile have yet to be reported. Here we show that injecting shrimp with oxytetracycline and oxolinic acid alters the expression of genes in the black tiger shrimp, Penaeus monodon, lymphoid organ. These antibiotics, especially oxylinic acid, downregulated the expression of a few immune-related genes, most notably penaeidin, proPO, clotting protein, profilin and whey acidic protein.

  • comparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon
    BMC Genomics, 2007
    Co-Authors: Chih Chin Chang, Jinlu Wu, Ikuo Hirono, Takashi Aoki, Hsuehfen Juan, Chu Fang Lo, Hsuancheng Huang
    Abstract:

    Background White spot syndrome (WSS) is a viral disease that affects most of the commercially important shrimps and causes serious economic losses to the shrimp farming industry worldwide. However, little information is available in terms of the molecular mechanisms of the host-virus interaction. In this study, we used an expressed sequence tag (EST) approach to observe global gene expression changes in white spot syndrome virus (WSSV)-infected postlarvae of Penaeus monodon.

  • identification of immune related genes in hemocytes of black tiger shrimp Penaeus monodon
    Marine Biotechnology, 2002
    Co-Authors: Premruethai Supungul, Ikuo Hirono, Takashi Aoki, Sirawut Klinbunga, Sarawut Jitrapakdee, Rath Pichyangkura, Anchalee Tassanakajon
    Abstract:

    An expressed sequence tag (EST) library was constructed from hemocytes of the black tiger shrimp (Penaeus monodon) to identify genes associated with immunity in this economically important species. The number of complementary DNA clones in the constructed library was approximately 4 x 10(5). Of these, 615 clones having inserts larger than 500 bp were unidirectionally sequenced and analyzed by homology searches against data in GenBank. Significant homology to known genes was found in 314 (51%) of the 615 clones, but the remaining 301 sequences (49%) did not match any sequence in GenBank. Approximately 35% of the matched ESTs were significantly identified by the BLASTN and BLASTX programs, while 65% were recognized only by the BLASTX program. Of the 615 clones, 55 (8.9%) were identified as putative immune-related genes. The isolated genes were composed of those coding for enzymes and proteins in the clotting system and the prophenoloxidase-activating system, antioxidative enzymes, antimicrobial peptides, and serine proteinase inhibitors. Three full-length ESTs encoding antimicrobial peptides (antilipopolysaccharide and penaeidin homologues) and a heat shock protein (cpn10 homologue) are reported.

  • identification of immune related genes in hemocytes of black tiger shrimp Penaeus monodon
    Marine Biotechnology, 2002
    Co-Authors: Premruethai Supungul, Ikuo Hirono, Takashi Aoki, Sirawut Klinbunga, Sarawut Jitrapakdee, Rath Pichyangkura, Anchalee Tassanakajon
    Abstract:

    An expressed sequence tag (EST) library was constructed from hemocytes of the black tiger shrimp (Penaeus monodon) to identify genes associated with immunity in this economically important species. The number of complementary DNA clones in the constructed library was approximately 4 × 105. Of these, 615 clones having inserts larger than 500 by were unidirectionally sequenced and analyzed by homology searches against data in GenBank. Significant homology to known genes was found in 314 (51%) of the 615 clones, but the remaining 301 sequences (49%) did not match any sequence in GenBank. Approximately 35% of the matched ESTs were significantly identified by the BLASTN and BLASTX programs, while 65% were recognized only by the BLASTX program. Of the 615 clones, 55 (8.9%) were identified as putative immune-related genes. The isolated genes were composed of those coding for enzymes and proteins in the clotting system and the prophenoloxidase-activating system, antioxidative enzymes, antimicrobial peptides, and serine proteinase inhibitors. Three full-length ESTs encoding antimicrobial peptides (antilipopolysaccharide and penaeidin homologues) and a heat shock protein (cpn10 homologue) are reported.

Iddya Karunasagar - One of the best experts on this subject based on the ideXlab platform.

  • Detection of monodon baculovirus and white spot syndrome virus in apparently healthy Penaeus monodon postlarvae from India by polymerase chain reaction
    Aquaculture, 2002
    Co-Authors: S.k. Otta, Iddya Karunasagar
    Abstract:

    The simultaneous presence of monodon baculovirus (MBV) and whitespot syndrome virus (WSSV) in apparently healthy postlarvae of Penaeus monodon from different hatcheries in India was studied by nested polymerase chain reaction (PCR). MBV could be detected in 54% of the samples. However, only 15% of samples were positive by non-nested reaction. WSSV could be detected in 75% of samples, 19% being positive by non-nested reaction. The results show simultaneous presence of WSSV and MBV in many samples at various degrees of infection. Only 14% of the samples analysed were negative for both viruses. D 2003 Elsevier Science B.V. All rights reserved.

  • Multiple viral infection in Penaeus monodon shrimp postlarvae in an Indian hatchery.
    Diseases of aquatic organisms, 2002
    Co-Authors: Subramaniyan Manivannan, S.k. Otta, Iddya Karunasagar
    Abstract:

    Moribund Penaeus monodon postlarvae (PL8-PL10) in a hatchery in India were found to be simultaneously infected by 3 different viruses. They were highly infected with monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) by histology and with white spot syndrome virus (WSSV) by non-nested polymerase chain reaction (PCR). Apparently healthy postlarvae tested from the same hatchery were positive for MBV and WSSV by nested PCR only. Tissue sections of such postlarvae did not show any histopathological changes. The simultaneous occurrence of these 3 viruses in hatchery-reared postlarval P. monodon is being reported for the first time.

  • mass mortality of Penaeus monodon larvae due to antibiotic resistant vibrio harveyi infection
    Aquaculture, 1994
    Co-Authors: R Pai, G R Malathi, Iddya Karunasagar
    Abstract:

    Abstract The cause of mass mortality in Penaeus monodon larvae in a hatchery was investigated. Antibiotic-resistant Vibrio harveyi could be isolated from all the infected larvae. These bacteria were absent in healthy eggs and nauplii. Although the intake seawater had V. harveyi , these strains were sensitive to antibiotics. The results suggest that antibiotic-resistant V. harveyi had been colonising larval tanks. The isolates from moribund larvae showed much lower LD 50 values than isolates from natural seawater, thus indicating their higher virulence.

Kallaya Sritunyalucksana - One of the best experts on this subject based on the ideXlab platform.

  • Penaeus monodon gene discovery project the generation of an est collection and establishment of a database
    Gene, 2006
    Co-Authors: Anchalee Tassanakajon, Kallaya Sritunyalucksana, Sirawut Klinbunga, Narongsak Paunglarp, Vichien Rimphanitchayakit, Apinun Udomkit, Sarawut Jitrapakdee
    Abstract:

    Abstract A large-scale expressed sequence tag (EST) sequencing project was undertaken for the purpose of gene discovery in the black tiger shrimp Penaeus monodon. Initially, 15 cDNA libraries were constructed from different tissues (eyestalk, hepatopancrease, haematopoietic tissue, haemocyte, lymphoid organ, and ovary) of shrimp, reared under normal or stress conditions, to identify tissue-specific genes and genes responding to infection and heat stress. A total of 10,100 clones were analyzed by single-pass sequencing from the 5′ end. Clustering and assembling of these ESTs resulted in a total of 4845 unique sequences with 917 overlapping contigs and 3928 singletons. The redundancy of each cDNA library ranged from 13.4% to 61.3% with an overall redundancy of 61.1%. About half of these ESTs (2365 clones, 48.8%) showed significant homology (BLASTX, e-values http://pmodon.biotec.or.th ). This EST database provides a useful resource for gene identification and functional genomic studies of shrimp.

  • Penaeus monodon gene discovery project the generation of an est collection and establishment of a database
    Gene, 2006
    Co-Authors: Anchalee Tassanakajon, Kallaya Sritunyalucksana, Sirawut Klinbunga, Narongsak Paunglarp, Vichien Rimphanitchayakit, Apinun Udomkit, Sarawut Jitrapakdee
    Abstract:

    Abstract A large-scale expressed sequence tag (EST) sequencing project was undertaken for the purpose of gene discovery in the black tiger shrimp Penaeus monodon. Initially, 15 cDNA libraries were constructed from different tissues (eyestalk, hepatopancrease, haematopoietic tissue, haemocyte, lymphoid organ, and ovary) of shrimp, reared under normal or stress conditions, to identify tissue-specific genes and genes responding to infection and heat stress. A total of 10,100 clones were analyzed by single-pass sequencing from the 5′ end. Clustering and assembling of these ESTs resulted in a total of 4845 unique sequences with 917 overlapping contigs and 3928 singletons. The redundancy of each cDNA library ranged from 13.4% to 61.3% with an overall redundancy of 61.1%. About half of these ESTs (2365 clones, 48.8%) showed significant homology (BLASTX, e-values http://pmodon.biotec.or.th ). This EST database provides a useful resource for gene identification and functional genomic studies of shrimp.

  • a new rna virus found in black tiger shrimp Penaeus monodon from thailand
    Virus Research, 2006
    Co-Authors: Kallaya Sritunyalucksana, Somjai Apisawetakan, Anutara Boonnat, Boonsirm Withyachumnarnkul, T W Flegel
    Abstract:

    A new, apparently innocuous virus was found while investigating the cause of monodon slow growth syndrome (MSGS) in cultured black tiger shrimp (Penaeus monodon). It was identified via plasmid vector clones of E. coli containing randomly amplified cDNA fragments produced from total nucleic acid extracts of hemolymph from MSGS shrimp. Of 421 clones, 30 that failed to give positive dot blot hybridization with a digoxigenin (DIG)-labeled shrimp DNA probe were sequenced and compared to GenBank records. Of these, 22 corresponded to known shrimp DNA records. Of eight that did not, one (20A) showed significant deduced amino acid sequence similarity to RNA-dependent RNA polymerases (RdRp) of the viruses in the family Luteoviridae and alignment revealed commonly conserved amino acids including a GDD motif believed to be at the enzyme active site. However, phylogenetic analysis showed that the virus sequence did not cluster with the Luteoviridae or other known RNA virus sequences. Thus, in accordance with frequent practice, it was named according to the area where it was first collected as Laem-Singh virus (LSNV). In situ hybridization with a DIG-labeled 20A insert revealed strong cytoplasmic staining confined to the lymphoid organ (LO), the heart and hepatopancreatic connective tissue in both normal and MSGS shrimp. RT-PCR assays based on the 20A clone sequence also gave positive results with both normal and MSGS shrimp. Transmission electron microscopy (TEM) of LO tissue revealed viral-like particles of approximately 27 nm diameter (within the Luteoviridae size range) in locations that matched those of positive in situ hybridization reactions in parallel samples. Although not directly associated with MSGS in Penaeus monodon, the presence or effect of this virus with other crustacean species is presently unknown.

  • molecular cloning and characterization of prophenoloxidase in the black tiger shrimp Penaeus monodon
    Developmental and Comparative Immunology, 1999
    Co-Authors: Kallaya Sritunyalucksana, Lage Cerenius, Kenneth Soderhall
    Abstract:

    A cDNA encoding shrimp, Penaeus monodon, prophenoloxidase (proPO) was obtained by screening a hemocyte library by plaque hybridization using a proPO cDNA fragment from freshwater crayfish, Pacifastacus leniusculus, as a probe. The 3,002 bp cDNA contains an open reading frame of 2,121 bp and a 881 bp 3′-untranslated region. The molecular mass of the deduced amino acid sequence (688 amino acids) is 78,700 Da with an estimated pI of 5.8. Two putative copper binding sites are present and they have a highly conserved sequence around these sites. No signal peptide was detected in the shrimp proPO, as has been previously shown to be the case for all arthropod proPOs cloned so far. The cleavage site of zymogen activation is likely to be between Arg 44 and Val 45. A tentative complement-like motif (GCGWPQHM) is also present. Shrimp proPO mRNA is synthesized in the hemocytes and not in the hepatopancreas. Comparison of amino acid sequences showed that shrimp proPO is more closely related to another crustacean proPO, namely crayfish, than to the insect proPOs.

Kongkarn Kijroongrojana - One of the best experts on this subject based on the ideXlab platform.