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Yulong Li - One of the best experts on this subject based on the ideXlab platform.

  • biocontrol effects of Penicillium griseofulvum against monkshood aconitum carmichaelii debx root diseases caused by sclerotium rolfsiii and fusarium spp
    Journal of Applied Microbiology, 2019
    Co-Authors: Yulong Li
    Abstract:

    Aims The aims of this study were to investigate the biocontrol effects of Penicillium griseofulvum strain CF3 and its mechanisms against soil-borne root pathogens (Fusarium oxysporum and Sclerotium rolfsii) of the medical plant Aconitum carmichaelii Debx. Methods and results The effects of P. griseofulvum strain CF3 were evaluated with regard to the hyphal growth of S. rolfsii and F. oxysporum, the sclerotial formation and germination of S. rolfsii and its expression of sclerotia-formation-related genes. A field experiment was conducted to explore how strain CF3 controls the severity of soil-borne diseases, promotes the growth of A. carmichaelii plants and mediates shifts in the culturable rhizosphere microbial populations. The results showed that treatment with a cell-free culture filtrate of strain CF3 considerably inhibited the hyphal growth of both S. rolfsii and F. oxysporum, in addition to limiting the sclerotial formation and germination of S. rolfsii. Three genes related to sclerotial formation (ArsclR, ArnsdD1 and ArnsdD2) were predicted in S. rolfsii and their expression was found suppressed by the CF3 treatment. Field application of the CF3 biocontrol agent in a powder form (1·9 × 1010 conidia per gram of substrate) reduced soil-borne disease severity by 15·0%. The shoot and root growth of A. carmichaelii plants was promoted by 61·6 and 83·1% respectively, as the biocontrol strain massively colonized the rhizosphere soil. The CF3 treatment also markedly reduced the density of some known species harmful to plants while increasing the density of some beneficial species in the rhizosphere soil. Significance and impact of the study Genes related to sclerotia formation of S. rolfsii are predicted for the first time and their expression patterns in the presence of P. griseofulvum strain CF3 are evaluated. This comprehensive study provides a candidate fungal biocontrol strain and reveals its potential mechanisms against S. rolfsii and F. oxysporum in A. carmichaelii plants.

  • biocontrol effects of Penicillium griseofulvum against monkshood aconitum carmichaelii debx root diseases caused by sclerotium rolfsiii and fusarium spp
    Journal of Applied Microbiology, 2019
    Co-Authors: Yulong Li
    Abstract:

    AIMS: The aims of this study were to investigate the biocontrol effects of Penicillium griseofulvum strain CF3 and its mechanisms against soil-borne root pathogens (Fusarium oxysporum and Sclerotium rolfsii) of the medical plant Aconitum carmichaelii Debx. METHODS AND RESULTS: The effects of P. griseofulvum strain CF3 were evaluated with regard to the hyphal growth of S. rolfsii and F. oxysporum, the sclerotial formation and germination of S. rolfsii and its expression of sclerotia-formation-related genes. A field experiment was conducted to explore how strain CF3 controls the severity of soil-borne diseases, promotes the growth of A. carmichaelii plants and mediates shifts in the culturable rhizosphere microbial populations. The results showed that treatment with a cell-free culture filtrate of strain CF3 considerably inhibited the hyphal growth of both S. rolfsii and F. oxysporum, in addition to limiting the sclerotial formation and germination of S. rolfsii. Three genes related to sclerotial formation (ArsclR, ArnsdD1 and ArnsdD2) were predicted in S. rolfsii and their expression was found suppressed by the CF3 treatment. Field application of the CF3 biocontrol agent in a powder form (1·9 × 1010 conidia per gram of substrate) reduced soil-borne disease severity by 15·0%. The shoot and root growth of A. carmichaelii plants was promoted by 61·6 and 83·1% respectively, as the biocontrol strain massively colonized the rhizosphere soil. The CF3 treatment also markedly reduced the density of some known species harmful to plants while increasing the density of some beneficial species in the rhizosphere soil. SIGNIFICANCE AND IMPACT OF THE STUDY: Genes related to sclerotia formation of S. rolfsii are predicted for the first time and their expression patterns in the presence of P. griseofulvum strain CF3 are evaluated. This comprehensive study provides a candidate fungal biocontrol strain and reveals its potential mechanisms against S. rolfsii and F. oxysporum in A. carmichaelii plants.

  • Biocontrol effects of Penicillium griseofulvum against monkshood (Aconitum carmichaelii Debx.) root diseases caused by Sclerotium rolfsiii and Fusarium spp.
    Journal of Applied Microbiology, 2019
    Co-Authors: Yulong Li
    Abstract:

    The aims of this study were to investigate the biocontrol effects of Penicillium griseofulvum strain CF3 and its mechanisms against soil-borne root pathogens (Fusarium oxysporum and Sclerotium rolfsii) of the medical plant Aconitum carmichaelii Debx. The effects of P. griseofulvum strain CF3 were evaluated with regard to the hyphal growth of S. rolfsii and F. oxysporum, the sclerotial formation and germination of S. rolfsii and its expression of sclerotia-formation-related genes. A field experiment was conducted to explore how strain CF3 controls the severity of soil-borne diseases, promotes the growth of A. carmichaelii plants and mediates shifts in the culturable rhizosphere microbial populations. The results showed that treatment with a cell-free culture filtrate of strain CF3 considerably inhibited the hyphal growth of both S. rolfsii and F. oxysporum, in addition to limiting the sclerotial formation and germination of S. rolfsii. Three genes related to sclerotial formation (ArsclR, ArnsdD1 and ArnsdD2) were predicted in S. rolfsii and their expression was found suppressed by the CF3 treatment. Field application of the CF3 biocontrol agent in a powder form (1·9 × 1010 conidia per gram of substrate) reduced soil-borne disease severity by 15·0%. The shoot and root growth of A. carmichaelii plants was promoted by 61·6 and 83·1% respectively, as the biocontrol strain massively colonized the rhizosphere soil. The CF3 treatment also markedly reduced the density of some known species harmful to plants while increasing the density of some beneficial species in the rhizosphere soil. Genes related to sclerotia formation of S. rolfsii are predicted for the first time and their expression patterns in the presence of P. griseofulvum strain CF3 are evaluated. This comprehensive study provides a candidate fungal biocontrol strain and reveals its potential mechanisms against S. rolfsii and F. oxysporum in A. carmichaelii plants. © 2019 The Society for Applied Microbiology.

Rekha S Singhal - One of the best experts on this subject based on the ideXlab platform.

  • Solid‐State Fermentation for Production of Griseofulvin on Rice Bran Using Penicillium griseofulvum
    Biotechnology Progress, 2008
    Co-Authors: Sayali S Saykhedkar, Rekha S Singhal
    Abstract:

    : Griseofulvin is a secondary metabolite produced from fungal species that have morphology suitable for solid-state fermentation (SSF). Reports on production of griseofulvin by SSF are scarce. The present work investigates SSF for griseofulvin production, optimization of its process parameters vis-a-vis the conventional submerged fermentation and its downstream processing from the same. Rice bran adjusted to an initial moisture content (IMC) of 50% (v/w) inoculated with 1 mL of a suspension of 10(6) spores/mL under agitation at 250 rpm containing the modified Czapek-Dox medium and additional 0.1% choline chloride as a precursor gave a yield of griseofulvin in 9 days that was comparable to submerged fermentation after 28 days. The yield of griseofulvin (microg/g dry biomass) was comparable in SSF and submerged fermentation. The biomass was estimated by estimation of chitin. Discussions on the effect of each parameter in SSF have also been included.

  • solid state fermentation for production of griseofulvin on rice bran using Penicillium griseofulvum
    Biotechnology Progress, 2004
    Co-Authors: Sayali S Saykhedkar, Rekha S Singhal
    Abstract:

    : Griseofulvin is a secondary metabolite produced from fungal species that have morphology suitable for solid-state fermentation (SSF). Reports on production of griseofulvin by SSF are scarce. The present work investigates SSF for griseofulvin production, optimization of its process parameters vis-a-vis the conventional submerged fermentation and its downstream processing from the same. Rice bran adjusted to an initial moisture content (IMC) of 50% (v/w) inoculated with 1 mL of a suspension of 10(6) spores/mL under agitation at 250 rpm containing the modified Czapek-Dox medium and additional 0.1% choline chloride as a precursor gave a yield of griseofulvin in 9 days that was comparable to submerged fermentation after 28 days. The yield of griseofulvin (microg/g dry biomass) was comparable in SSF and submerged fermentation. The biomass was estimated by estimation of chitin. Discussions on the effect of each parameter in SSF have also been included.

Ramalingam Chidambaram - One of the best experts on this subject based on the ideXlab platform.

  • hexavalent chromium biosorption studies using Penicillium griseofulvum msr1 a novel isolate from tannery effluent site box behnken optimization equilibrium kinetics and thermodynamic studies
    Journal of The Taiwan Institute of Chemical Engineers, 2015
    Co-Authors: Evy Alice Abigail M, Melvin S Samuel, Ramalingam Chidambaram
    Abstract:

    Abstract This study reports the biosorption of Cr(VI) using a new indigenous fungal biosorbent Penicillium griseofulvum MSR1, which was isolated from locally sourced tannery effluent site. Response surface methodology based on Box–Behnken design (BBD) was used for optimizing the key variables viz. biosorbent dosage, initial Cr(VI) concentration and contact time. The results of BBD showed maximum biosorption of about 79.9% at 2 g/L biosorbent dosage, 67.8 mg/L initial Cr(VI) concentration and at contact time of 37.5 min. Langmuir isotherm model fitted better to the obtained equilibrium data with maximum adsorption capacity of about 75.1 mg/g and the pseudo-second order model best described the biosorption kinetics. Thermodynamic parameters revealed that the process was spontaneous, feasible and endothermic in nature. Desorption studies revealed that the MSR1 biosorbent can be regenerated using 0.1 M HNO 3 and reused for further biosorption studies. The result implies that MSR1 is a cheap and promising biosorbent for the removal of Cr(VI) from wastewater.

  • Hexavalent chromium biosorption studies using Penicillium griseofulvum MSR1 a novel isolate from tannery effluent site: Box–Behnken optimization, equilibrium, kinetics and thermodynamic studies
    Journal of The Taiwan Institute of Chemical Engineers, 2015
    Co-Authors: Evy Alice Abigail M, Melvin S Samuel, Ramalingam Chidambaram
    Abstract:

    Abstract This study reports the biosorption of Cr(VI) using a new indigenous fungal biosorbent Penicillium griseofulvum MSR1, which was isolated from locally sourced tannery effluent site. Response surface methodology based on Box–Behnken design (BBD) was used for optimizing the key variables viz. biosorbent dosage, initial Cr(VI) concentration and contact time. The results of BBD showed maximum biosorption of about 79.9% at 2 g/L biosorbent dosage, 67.8 mg/L initial Cr(VI) concentration and at contact time of 37.5 min. Langmuir isotherm model fitted better to the obtained equilibrium data with maximum adsorption capacity of about 75.1 mg/g and the pseudo-second order model best described the biosorption kinetics. Thermodynamic parameters revealed that the process was spontaneous, feasible and endothermic in nature. Desorption studies revealed that the MSR1 biosorbent can be regenerated using 0.1 M HNO 3 and reused for further biosorption studies. The result implies that MSR1 is a cheap and promising biosorbent for the removal of Cr(VI) from wastewater.

Donald T Wicklow - One of the best experts on this subject based on the ideXlab platform.

Sayali S Saykhedkar - One of the best experts on this subject based on the ideXlab platform.

  • Solid‐State Fermentation for Production of Griseofulvin on Rice Bran Using Penicillium griseofulvum
    Biotechnology Progress, 2008
    Co-Authors: Sayali S Saykhedkar, Rekha S Singhal
    Abstract:

    : Griseofulvin is a secondary metabolite produced from fungal species that have morphology suitable for solid-state fermentation (SSF). Reports on production of griseofulvin by SSF are scarce. The present work investigates SSF for griseofulvin production, optimization of its process parameters vis-a-vis the conventional submerged fermentation and its downstream processing from the same. Rice bran adjusted to an initial moisture content (IMC) of 50% (v/w) inoculated with 1 mL of a suspension of 10(6) spores/mL under agitation at 250 rpm containing the modified Czapek-Dox medium and additional 0.1% choline chloride as a precursor gave a yield of griseofulvin in 9 days that was comparable to submerged fermentation after 28 days. The yield of griseofulvin (microg/g dry biomass) was comparable in SSF and submerged fermentation. The biomass was estimated by estimation of chitin. Discussions on the effect of each parameter in SSF have also been included.

  • solid state fermentation for production of griseofulvin on rice bran using Penicillium griseofulvum
    Biotechnology Progress, 2004
    Co-Authors: Sayali S Saykhedkar, Rekha S Singhal
    Abstract:

    : Griseofulvin is a secondary metabolite produced from fungal species that have morphology suitable for solid-state fermentation (SSF). Reports on production of griseofulvin by SSF are scarce. The present work investigates SSF for griseofulvin production, optimization of its process parameters vis-a-vis the conventional submerged fermentation and its downstream processing from the same. Rice bran adjusted to an initial moisture content (IMC) of 50% (v/w) inoculated with 1 mL of a suspension of 10(6) spores/mL under agitation at 250 rpm containing the modified Czapek-Dox medium and additional 0.1% choline chloride as a precursor gave a yield of griseofulvin in 9 days that was comparable to submerged fermentation after 28 days. The yield of griseofulvin (microg/g dry biomass) was comparable in SSF and submerged fermentation. The biomass was estimated by estimation of chitin. Discussions on the effect of each parameter in SSF have also been included.