The Experts below are selected from a list of 1029 Experts worldwide ranked by ideXlab platform
Mukund Adsul - One of the best experts on this subject based on the ideXlab platform.
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induction of cellulases by disaccharides or their derivatives in Penicillium janthinellum ems uv 8 mutant
Biofuels, 2017Co-Authors: Ruchi Agrawal, Anshu S Mathur, Ravi P Gupta, Deepak Kumar Tuli, Alok Satlewal, Bhawna Sharma, Mukund AdsulAbstract:ABSTRACTDisaccharides or their derivatives were analyzed for the induction of cellulases by Penicillium janthinellum EMS-UV-8 mutant. Among seven (Crocin, Gentiobiose, Cellobiose, α-D-Cellobiose octaacetate, Trehalose, Melibiose, Turanose) different compounds tested, α-D-Cellobiose octaacetate (COA) was found to be promising for induction of cellulases. The maximum of 0.8 IU/mL filter paper units, 4.7 IU/mL β–glucosidase and 1.0 mg/mL protein were produced using 0.1% (weight/volume) COA as an inducer and 1% glucose as a carbon source for growth. Next to COA the gentiobiose, trehalose, melibiose and turanose also induce cellulase but at a low level. The induction of cellulase was also analyzed in the presence and absence of light and it was found that the absence of light supports more growth and less cellulase induction, while in the presence of light mycelial growth was restricted but more cellulases were induced. The knowledge of cellulase induction by COA and light could be beneficial for large-scale c...
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untreated wheat straw potential source for diverse cellulolytic enzyme secretion by Penicillium janthinellum ems uv 8 mutant
Bioresource Technology, 2015Co-Authors: Bhawna Sharma, Reeta Rani Singhania, Anshu S Mathur, Deepak Kumar Tuli, Ruchi Agrawal, Alok Satlewal, Mukund AdsulAbstract:Study describes the production of cellulases by Penicillium janthinellum EMS-UV-8 using untreated wheat straw (WS), treated WS (acid, alkali, steam exploded, organo-solv) and pure cellulosic substrates (avicel, cellulose-II and carboxymethyl cellulose). Severely pretreated WS and cellulose-II produced more cellulolytic enzymes than untreated samples. XRD and FTIR analysis revels that the increase in the amorphous structure of pretreated WS/cellulose increases enzyme production. Enzyme samples prepared using different substrates were used for the hydrolysis of dilute acid treated wheat straw (DATWS), steam exploded wheat straw (SEWS) and avicel. The enzyme prepared using untreated WS gave more hydrolysis of DATWS and SEWS than the enzyme prepared using pretreated WS or pure cellulosic substrates. This revels that more diverse/potential enzymes were secreted by P. janthinellum EMS-UV-8 mutant using untreated WS. This study may contribute in production of efficient enzyme mixture/cocktail by single fungal strain for economic conversion of biomass to sugars.
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bioethanol production from wheat straw via enzymatic route employing Penicillium janthinellum cellulases
Bioresource Technology, 2014Co-Authors: Reeta Rani Singhania, Mukund Adsul, Jitendra Kumar Saini, Reetu Saini, Anshu S Mathur, Ravi P Gupta, Deepak Kumar TuliAbstract:This study concerns in-house development of cellulases from a mutant Penicillium janthinellum EMS-UV-8 and its application in separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes for bioethanol production from pre-treated wheat straw. In a 5 L fermentor, the above strain could produce cellulases having activity of 3.1 FPU/mL and a specific activity of 0.83 FPU/mg of protein. In-house developed cellulase worked more efficiently in case of SSF as ethanol concentration of 21.6 g/L and yield of 54.4% were obtained which were higher in comparison to SHF (ethanol concentration 12 g/L and 30.2% yield). This enzyme preparation when compared with commercial cellulase for hydrolysis of pre-treated wheat straw was found competitive. This study demonstrates that P. janthinellum EMS-UV-8 is a potential fungus for future large-scale production of cellulases.
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comparative production of cellulases by mutants of Penicillium janthinellum ncim 1171 and its application in hydrolysis of avicel and cellulose
Bioresource Technology, 2011Co-Authors: Mamta Singhvi, Mukund Adsul, D V GokhaleAbstract:Mutants of Penicillium janthinellum NCIM 1171 were evaluated for cellulase production using both submerged fermentation (SmF) and solid state fermentation (SSF). Mutant EU2D-21 gave highest yields of cellulases in both SmF and SSF. Hydrolysis of Avicel and cellulose were compared using SmF and SSF derived enzyme preparations obtained from EU2D-21. Surprisingly, the use of SSF derived preparation gave less hydrolysis compared to SmF derived enzymes. This may be due to inactivation of β-glucosidase at 50 °C in SSF derived enzyme preparations. SmF derived enzyme preparations contained both thermostable and thermosensitive β-glucosidases where as SSF derived enzyme preparations contained predominantly thermosensitive β-glucosidase. This is the first report on less thermostability of SSF derived β-glucosidase which is the main reason for getting less hydrolysis.
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strain improvement of Penicillium janthinellum ncim 1171 for increased cellulase production
Bioresource Technology, 2007Co-Authors: Mukund Adsul, K B Bastawde, Anjani J Varma, D V GokhaleAbstract:The strain of Penicillium janthinellum NCIM 1171 was subjected to mutation involving treatment of Ethyl Methyl Sulfonate (EMS) for 24h followed by UV-irradiation for 3min. Successive mutants showed enhanced cellulase production (EMS-UV-8), clearance zone on Avicel containing plate (SM2) and rapid growth on Walseth cellulose agar plates containing 0.2% 2-deoxy-d-glucose (SM3). These mutants were transferred to Walseth cellulose plates containing higher concentration (1.5%) of 2-deoxy-d-glucose (SM4) in which only five mutants showed clearance zone on SM4. All these mutants showed approximately two-fold increase in activity of both FPase and CMCase in shake flask culture when grown on basal medium containing CP-123 (1%) and wheat bran (2.5%). The enzyme preparations from these mutants were used to hydrolyze Avicel. Higher hydrolysis yields of Avicel were obtained with enzyme preparations of EU1. This is the first report on the isolation and selection of mutants based on hydrolysis of Avicel, which is the most crystalline substrate.
Deepak Kumar Tuli - One of the best experts on this subject based on the ideXlab platform.
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induction of cellulases by disaccharides or their derivatives in Penicillium janthinellum ems uv 8 mutant
Biofuels, 2017Co-Authors: Ruchi Agrawal, Anshu S Mathur, Ravi P Gupta, Deepak Kumar Tuli, Alok Satlewal, Bhawna Sharma, Mukund AdsulAbstract:ABSTRACTDisaccharides or their derivatives were analyzed for the induction of cellulases by Penicillium janthinellum EMS-UV-8 mutant. Among seven (Crocin, Gentiobiose, Cellobiose, α-D-Cellobiose octaacetate, Trehalose, Melibiose, Turanose) different compounds tested, α-D-Cellobiose octaacetate (COA) was found to be promising for induction of cellulases. The maximum of 0.8 IU/mL filter paper units, 4.7 IU/mL β–glucosidase and 1.0 mg/mL protein were produced using 0.1% (weight/volume) COA as an inducer and 1% glucose as a carbon source for growth. Next to COA the gentiobiose, trehalose, melibiose and turanose also induce cellulase but at a low level. The induction of cellulase was also analyzed in the presence and absence of light and it was found that the absence of light supports more growth and less cellulase induction, while in the presence of light mycelial growth was restricted but more cellulases were induced. The knowledge of cellulase induction by COA and light could be beneficial for large-scale c...
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untreated wheat straw potential source for diverse cellulolytic enzyme secretion by Penicillium janthinellum ems uv 8 mutant
Bioresource Technology, 2015Co-Authors: Bhawna Sharma, Reeta Rani Singhania, Anshu S Mathur, Deepak Kumar Tuli, Ruchi Agrawal, Alok Satlewal, Mukund AdsulAbstract:Study describes the production of cellulases by Penicillium janthinellum EMS-UV-8 using untreated wheat straw (WS), treated WS (acid, alkali, steam exploded, organo-solv) and pure cellulosic substrates (avicel, cellulose-II and carboxymethyl cellulose). Severely pretreated WS and cellulose-II produced more cellulolytic enzymes than untreated samples. XRD and FTIR analysis revels that the increase in the amorphous structure of pretreated WS/cellulose increases enzyme production. Enzyme samples prepared using different substrates were used for the hydrolysis of dilute acid treated wheat straw (DATWS), steam exploded wheat straw (SEWS) and avicel. The enzyme prepared using untreated WS gave more hydrolysis of DATWS and SEWS than the enzyme prepared using pretreated WS or pure cellulosic substrates. This revels that more diverse/potential enzymes were secreted by P. janthinellum EMS-UV-8 mutant using untreated WS. This study may contribute in production of efficient enzyme mixture/cocktail by single fungal strain for economic conversion of biomass to sugars.
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bioethanol production from wheat straw via enzymatic route employing Penicillium janthinellum cellulases
Bioresource Technology, 2014Co-Authors: Reeta Rani Singhania, Mukund Adsul, Jitendra Kumar Saini, Reetu Saini, Anshu S Mathur, Ravi P Gupta, Deepak Kumar TuliAbstract:This study concerns in-house development of cellulases from a mutant Penicillium janthinellum EMS-UV-8 and its application in separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes for bioethanol production from pre-treated wheat straw. In a 5 L fermentor, the above strain could produce cellulases having activity of 3.1 FPU/mL and a specific activity of 0.83 FPU/mg of protein. In-house developed cellulase worked more efficiently in case of SSF as ethanol concentration of 21.6 g/L and yield of 54.4% were obtained which were higher in comparison to SHF (ethanol concentration 12 g/L and 30.2% yield). This enzyme preparation when compared with commercial cellulase for hydrolysis of pre-treated wheat straw was found competitive. This study demonstrates that P. janthinellum EMS-UV-8 is a potential fungus for future large-scale production of cellulases.
D V Gokhale - One of the best experts on this subject based on the ideXlab platform.
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bacterial biofilm degradation using extracellular enzymes produced by Penicillium janthinellum eu2d 21 under submerged fermentation
Ai Magazine, 2018Co-Authors: Anil Kumar Nagraj, D V GokhaleAbstract:Bacterial biofilms are the bacterial aggregates that are embedded in the self-produced matrix of extracellular polymeric substances (EPS) that cause persistent bacterial infections posing significant medical challenges. They are recalcitrant to antibiotics and host defenses which make the treatments difficult and costly. Penicillium janthinellum mutant EU2D-21 was found to produce extracellular enzyme complex (amylase, cellulase, protease) under submerged fermentation. Maximum specific enzyme activities were found to be 3.04 IU/mg, 2.61 IU/mg and 3.39 IU/mg for alpha-amylase, cellulase and protease respectively, after 8 days of incubation at 30˚C. We evaluated the enzyme complex for its ability to target and degrade the biofilms of different bacteria. We found that it degraded biofilms of Escherichia coli (85.5%), Salmonella enterica (79.72%), Pseudomonas aeruginosa (88.76%) and Staphyloccus aureus (87.42%) within 1 h of incubation at 50˚C. The scanning electron microscopy (SEM), quantitation of biofilm removal assay and Crystal violet assay demonstrated that the enzyme complex detached the biofilm exo-polysaccharide matrix and bacteria from the cell surface. These results illustrate the feasibility and benefits of using this enzyme complex as anti-biofilm therapeutics to eradicate biofilms. This can also be used as a promising strategy to improve treatment of multidrug resistant bacterial infections.
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optimization studies for enhancing cellulase production by Penicillium janthinellum mutant eu2d 21 using response surface methodology
Bioresources, 2014Co-Authors: Anil Kumar Nagraj, Mamata Singhvi, Ravi V Kumar, D V GokhaleAbstract:Extracellular fungal cellulases are key enzymes for the degradation of lignocellulosic biomass. Greater production of these enzymes could reduce the cost of biofuels production. In this study, the basal medium for cellulase production by a Penicillium janthinellum mutant (EU2D-21) in submerged fermentation conditions was optimized using response surface methodology (RSM). Initial studies using a Plackett-Burman design (PBD) showed that (NH4)2SO4 and urea are significant factors for improving β-glucosidase and FPase production. A central composite design (CCD) was applied to obtain the maximum response, which resulted in the optimal production of β-glucosidase (5.79 IU/mL) and FPase (5.76 IU/mL). These values were 1.87 and 1.67 times higher than the corresponding values obtained under un-optimized conditions.
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comparative production of cellulases by mutants of Penicillium janthinellum ncim 1171 and its application in hydrolysis of avicel and cellulose
Bioresource Technology, 2011Co-Authors: Mamta Singhvi, Mukund Adsul, D V GokhaleAbstract:Mutants of Penicillium janthinellum NCIM 1171 were evaluated for cellulase production using both submerged fermentation (SmF) and solid state fermentation (SSF). Mutant EU2D-21 gave highest yields of cellulases in both SmF and SSF. Hydrolysis of Avicel and cellulose were compared using SmF and SSF derived enzyme preparations obtained from EU2D-21. Surprisingly, the use of SSF derived preparation gave less hydrolysis compared to SmF derived enzymes. This may be due to inactivation of β-glucosidase at 50 °C in SSF derived enzyme preparations. SmF derived enzyme preparations contained both thermostable and thermosensitive β-glucosidases where as SSF derived enzyme preparations contained predominantly thermosensitive β-glucosidase. This is the first report on less thermostability of SSF derived β-glucosidase which is the main reason for getting less hydrolysis.
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strain improvement of Penicillium janthinellum ncim 1171 for increased cellulase production
Bioresource Technology, 2007Co-Authors: Mukund Adsul, K B Bastawde, Anjani J Varma, D V GokhaleAbstract:The strain of Penicillium janthinellum NCIM 1171 was subjected to mutation involving treatment of Ethyl Methyl Sulfonate (EMS) for 24h followed by UV-irradiation for 3min. Successive mutants showed enhanced cellulase production (EMS-UV-8), clearance zone on Avicel containing plate (SM2) and rapid growth on Walseth cellulose agar plates containing 0.2% 2-deoxy-d-glucose (SM3). These mutants were transferred to Walseth cellulose plates containing higher concentration (1.5%) of 2-deoxy-d-glucose (SM4) in which only five mutants showed clearance zone on SM4. All these mutants showed approximately two-fold increase in activity of both FPase and CMCase in shake flask culture when grown on basal medium containing CP-123 (1%) and wheat bran (2.5%). The enzyme preparations from these mutants were used to hydrolyze Avicel. Higher hydrolysis yields of Avicel were obtained with enzyme preparations of EU1. This is the first report on the isolation and selection of mutants based on hydrolysis of Avicel, which is the most crystalline substrate.
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strain improvement of Penicillium janthinellum ncim 1171 for cellulase production
2007Co-Authors: Mukund Adsul, K B Bastawde, Anjani J Varma, D V GokhaleAbstract:The strain of Penicillium janthinellum NCIM 1171 was subjected to mutation involving treatment of Ethyl Methyl Sulfonate (EMS) for 24 h followed by UV-irradiation for 3 min. Successive mutants showed enhanced cellulase production (EMS-UV-8), clearance zone on Avicel containing plate (SM2) and rapid growth on Walseth cellulose agar plates containing 0.2% 2-deoxy-D-glucose (SM3). These mutants were transferred to Walseth cellulose plates containing higher concentration (1.5%) of 2-deoxy-D-glucose (SM4) in which only five mutants showed clearance zone on SM4. All these mutants showed approximately two-fold increase in activity of both FPase and CMCase in shake flask culture when grown on basal medium containing CP-123 (1%) and wheat bran (2.5%). The enzyme preparations from these mutants were used to hydrolyze Avicel. Higher hydrolysis yields of Avicel were obtained with enzyme preparations of EU1. This is the first report on the isolation and selection of mutants based on hydrolysis of Avicel, which is the most crystalline substrate.
Reeta Rani Singhania - One of the best experts on this subject based on the ideXlab platform.
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untreated wheat straw potential source for diverse cellulolytic enzyme secretion by Penicillium janthinellum ems uv 8 mutant
Bioresource Technology, 2015Co-Authors: Bhawna Sharma, Reeta Rani Singhania, Anshu S Mathur, Deepak Kumar Tuli, Ruchi Agrawal, Alok Satlewal, Mukund AdsulAbstract:Study describes the production of cellulases by Penicillium janthinellum EMS-UV-8 using untreated wheat straw (WS), treated WS (acid, alkali, steam exploded, organo-solv) and pure cellulosic substrates (avicel, cellulose-II and carboxymethyl cellulose). Severely pretreated WS and cellulose-II produced more cellulolytic enzymes than untreated samples. XRD and FTIR analysis revels that the increase in the amorphous structure of pretreated WS/cellulose increases enzyme production. Enzyme samples prepared using different substrates were used for the hydrolysis of dilute acid treated wheat straw (DATWS), steam exploded wheat straw (SEWS) and avicel. The enzyme prepared using untreated WS gave more hydrolysis of DATWS and SEWS than the enzyme prepared using pretreated WS or pure cellulosic substrates. This revels that more diverse/potential enzymes were secreted by P. janthinellum EMS-UV-8 mutant using untreated WS. This study may contribute in production of efficient enzyme mixture/cocktail by single fungal strain for economic conversion of biomass to sugars.
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bioethanol production from wheat straw via enzymatic route employing Penicillium janthinellum cellulases
Bioresource Technology, 2014Co-Authors: Reeta Rani Singhania, Mukund Adsul, Jitendra Kumar Saini, Reetu Saini, Anshu S Mathur, Ravi P Gupta, Deepak Kumar TuliAbstract:This study concerns in-house development of cellulases from a mutant Penicillium janthinellum EMS-UV-8 and its application in separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes for bioethanol production from pre-treated wheat straw. In a 5 L fermentor, the above strain could produce cellulases having activity of 3.1 FPU/mL and a specific activity of 0.83 FPU/mg of protein. In-house developed cellulase worked more efficiently in case of SSF as ethanol concentration of 21.6 g/L and yield of 54.4% were obtained which were higher in comparison to SHF (ethanol concentration 12 g/L and 30.2% yield). This enzyme preparation when compared with commercial cellulase for hydrolysis of pre-treated wheat straw was found competitive. This study demonstrates that P. janthinellum EMS-UV-8 is a potential fungus for future large-scale production of cellulases.
Anshu S Mathur - One of the best experts on this subject based on the ideXlab platform.
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induction of cellulases by disaccharides or their derivatives in Penicillium janthinellum ems uv 8 mutant
Biofuels, 2017Co-Authors: Ruchi Agrawal, Anshu S Mathur, Ravi P Gupta, Deepak Kumar Tuli, Alok Satlewal, Bhawna Sharma, Mukund AdsulAbstract:ABSTRACTDisaccharides or their derivatives were analyzed for the induction of cellulases by Penicillium janthinellum EMS-UV-8 mutant. Among seven (Crocin, Gentiobiose, Cellobiose, α-D-Cellobiose octaacetate, Trehalose, Melibiose, Turanose) different compounds tested, α-D-Cellobiose octaacetate (COA) was found to be promising for induction of cellulases. The maximum of 0.8 IU/mL filter paper units, 4.7 IU/mL β–glucosidase and 1.0 mg/mL protein were produced using 0.1% (weight/volume) COA as an inducer and 1% glucose as a carbon source for growth. Next to COA the gentiobiose, trehalose, melibiose and turanose also induce cellulase but at a low level. The induction of cellulase was also analyzed in the presence and absence of light and it was found that the absence of light supports more growth and less cellulase induction, while in the presence of light mycelial growth was restricted but more cellulases were induced. The knowledge of cellulase induction by COA and light could be beneficial for large-scale c...
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untreated wheat straw potential source for diverse cellulolytic enzyme secretion by Penicillium janthinellum ems uv 8 mutant
Bioresource Technology, 2015Co-Authors: Bhawna Sharma, Reeta Rani Singhania, Anshu S Mathur, Deepak Kumar Tuli, Ruchi Agrawal, Alok Satlewal, Mukund AdsulAbstract:Study describes the production of cellulases by Penicillium janthinellum EMS-UV-8 using untreated wheat straw (WS), treated WS (acid, alkali, steam exploded, organo-solv) and pure cellulosic substrates (avicel, cellulose-II and carboxymethyl cellulose). Severely pretreated WS and cellulose-II produced more cellulolytic enzymes than untreated samples. XRD and FTIR analysis revels that the increase in the amorphous structure of pretreated WS/cellulose increases enzyme production. Enzyme samples prepared using different substrates were used for the hydrolysis of dilute acid treated wheat straw (DATWS), steam exploded wheat straw (SEWS) and avicel. The enzyme prepared using untreated WS gave more hydrolysis of DATWS and SEWS than the enzyme prepared using pretreated WS or pure cellulosic substrates. This revels that more diverse/potential enzymes were secreted by P. janthinellum EMS-UV-8 mutant using untreated WS. This study may contribute in production of efficient enzyme mixture/cocktail by single fungal strain for economic conversion of biomass to sugars.
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bioethanol production from wheat straw via enzymatic route employing Penicillium janthinellum cellulases
Bioresource Technology, 2014Co-Authors: Reeta Rani Singhania, Mukund Adsul, Jitendra Kumar Saini, Reetu Saini, Anshu S Mathur, Ravi P Gupta, Deepak Kumar TuliAbstract:This study concerns in-house development of cellulases from a mutant Penicillium janthinellum EMS-UV-8 and its application in separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes for bioethanol production from pre-treated wheat straw. In a 5 L fermentor, the above strain could produce cellulases having activity of 3.1 FPU/mL and a specific activity of 0.83 FPU/mg of protein. In-house developed cellulase worked more efficiently in case of SSF as ethanol concentration of 21.6 g/L and yield of 54.4% were obtained which were higher in comparison to SHF (ethanol concentration 12 g/L and 30.2% yield). This enzyme preparation when compared with commercial cellulase for hydrolysis of pre-treated wheat straw was found competitive. This study demonstrates that P. janthinellum EMS-UV-8 is a potential fungus for future large-scale production of cellulases.