The Experts below are selected from a list of 7725 Experts worldwide ranked by ideXlab platform
Menglin Li - One of the best experts on this subject based on the ideXlab platform.
-
effects of anesthetics Pentobarbital Sodium and chloral hydrate on urine proteome
PeerJ, 2015Co-Authors: Mindi Zhao, Xundou Li, Menglin LiAbstract:Urine can be a better source than blood for biomarker discovery since it accumulates many changes. The urine proteome is susceptible to many factors, including anesthesia. Pentobarbital Sodium and chloral hydrate are commonly used anesthetics in animal experiments. This study demonstrated the effects of these two anesthetics on the rat urine proteome using liquid chromatography–tandem mass spectrometry (LC-MS/MS). With anesthesia, the urinary protein-to-creatinine ratio of all rats increased twofold. The relative abundance of 22 and 23 urinary proteins were changed with Pentobarbital Sodium or chloral hydrate anesthesia, respectively, as determined by label-free quantification. Among these changed proteins, fifteen had been considered as candidate biomarkers such as uromodulin, and sixteen had been considered stable in healthy human urine, which are more likely to be considered as potential biomarkers when changed, such as transferrin. The pattern of changed urinary proteins provides clues to the discovery of urinary proteins regulatory mechanisms. When determining a candidate biomarker, anesthetic-related effects can be excluded from future biomarker discovery studies. Since anesthetics take effects via nervous system, this study is the first to provide clues that the protein handling function of the kidney may possibly be regulated by the nervous system.
Mindi Zhao - One of the best experts on this subject based on the ideXlab platform.
-
effects of anesthetics Pentobarbital Sodium and chloral hydrate on urine proteome
PeerJ, 2015Co-Authors: Mindi Zhao, Xundou Li, Menglin LiAbstract:Urine can be a better source than blood for biomarker discovery since it accumulates many changes. The urine proteome is susceptible to many factors, including anesthesia. Pentobarbital Sodium and chloral hydrate are commonly used anesthetics in animal experiments. This study demonstrated the effects of these two anesthetics on the rat urine proteome using liquid chromatography–tandem mass spectrometry (LC-MS/MS). With anesthesia, the urinary protein-to-creatinine ratio of all rats increased twofold. The relative abundance of 22 and 23 urinary proteins were changed with Pentobarbital Sodium or chloral hydrate anesthesia, respectively, as determined by label-free quantification. Among these changed proteins, fifteen had been considered as candidate biomarkers such as uromodulin, and sixteen had been considered stable in healthy human urine, which are more likely to be considered as potential biomarkers when changed, such as transferrin. The pattern of changed urinary proteins provides clues to the discovery of urinary proteins regulatory mechanisms. When determining a candidate biomarker, anesthetic-related effects can be excluded from future biomarker discovery studies. Since anesthetics take effects via nervous system, this study is the first to provide clues that the protein handling function of the kidney may possibly be regulated by the nervous system.
-
Effects of anesthetics Pentobarbital Sodium and chloral hydrate on urine proteome
2015Co-Authors: Mindi Zhao, Youhe GaoAbstract:Background. Urine can be a better source than blood for biomarker discovery since it accumulates many changes. The urine proteome is susceptible to many factors including anesthesia. Pentobarbital Sodium and chloral hydrate are commonly used anesthetics in animal experiments. Methods. This study demonstrated effects of these two anesthetics on the rat urine proteome using liquid chromatography–tandem mass spectrometry (LC-MS/MS). Results. With anesthesia, the urinary protein-to-creatinine ratio of all rats increased two fold. The relative abundance of 22 and 23 urinary proteins were changed with Pentobarbital Sodium or chloral hydrate anesthesia, respectively, as determined by label-free quantification. Among these changed proteins, fifteen had been considered as candidate biomarkers such as uromodulin, sixteen had been considered stable in healthy human urine, which are more likely to be considered as potential biomarkers when changed, such as transferrin. Discussion. The pattern of changed urinary proteins provides clues to the discovery of urinary proteins regulatory mechanisms. When determining candidate biomarker, anesthetic-related effects can be excluded in future biomarker discovery studies. Since anesthetics take effects via nervous system, this study is the first to provide clues that protein handling function of kidney may possibly be regulated by nervous system.
Fukuko Kimura - One of the best experts on this subject based on the ideXlab platform.
-
Fos expression by naloxone in LHRH neurons of the mediobasal hypothalamus and effects of Pentobarbital Sodium in the proestrous rat.
Journal of neuroendocrinology, 1997Co-Authors: Toshiya Funabashi, Kayoko Jinnai, Fukuko KimuraAbstract:Because Fos is thought to be induced in neurons that are activated, we examined whether luteinizing hormone-releasing hormone (LHRH) neurons expressed Fos protein when they were stimulated by an opioid receptor antagonist naloxone (NAL), expecting to identify LHRH neurons which are regulated by opioid neurons directly or indirectly. Further, we examined whether an ovulation-blocking dosage of Pentobarbital Sodium (PB) would affect the NAL-induced Fos expression. Female rats were infused with naloxone (5 mg/kg/h) for 90 min (10.00-11.30) in the morning of proestrus, during which infusion blood sampling was done, and were killed by i.v. injection with an overdose of PB at 11.30-12.00. Dual immunoperoxidase/immunofluorescence staining for both Fos and LHRH revealed that some LHRH immunoreactive (ir) neurons in the forebrain expressed Fos-ir, associated with an increase in serum LH concentrations, but little co-localization was found in rats in proestrus which were infused with saline as the control. The proportion of LHRH-ir neurons which expressed Fos-ir was about 35-62% in the caudal part of the forebrain including the mediobasal hypothalamus, and this was larger than that (10%) in the rostral part of the forebrain including the preoptic area. PB injection (32 mg/kg bw, i.p.) 15 min prior to the beginning of NAL infusion significantly enhanced the increase in LH secretion due to NAL, and also enhanced Fos-ir expression in LHRH-ir neurons. Together with the well-established fact that PB blocks the LHRH surge generator and our previous findings that NAL stimulates the LHRH pulse generator even in the PB-blocked proestrous rat, these results strongly suggest that the LHRH pulse generator exists in the mediobasal hypothalamus which contains LHRH neurons that are responsive to NAL and express Fos protein.
-
Pentobarbital Sodium inhibits the release of noradrenaline in the medial preoptic area in the rat.
Neuroscience letters, 1994Co-Authors: Tooru M. Mizuno, Eriko Ito, Fukuko KimuraAbstract:Abstract The effect of Pentobarbital Sodium (PB) on noradrenaline (NA) release in the medial preoptic area was studied in ovariectomized rats by a microdialysis method coupled to HPLC. Dialyzates were collected at 6-min intervals before and after the injection in freely moving rats. PB injected i.p. at a dose of 35 mg/kg caused a marked, ∼40–50% decrease in NA release, which became evident 50 min after the injection and lasted for another 60 min during which the observation was performed. The results suggest that the anesthetic effect of barbiturates is related to the reduction in NA release in the brain.
Qian Cai - One of the best experts on this subject based on the ideXlab platform.
-
effect of eszopiclone on Pentobarbital Sodium induced sleeping time in acute hypobaric hypoxia mice
Chinese Journal of Behavioral Medicine and Brain Science, 2014Co-Authors: Ling Zhong, Yongbing Song, Jun Yang, Qian CaiAbstract:Objective To assess the effects of eszopiclone(ESZ) on the Pentobarbital Sodium-induced sleeping time and spontaneous activity in mice exposed to acute hypobaric hypoxia. Methods 120 mice were randomly divided into 6 groups by using two factors 2×3 levels factorial design, in which two factors were interventions (ESZ and 0.9% Sodium chloride, 2 levels) and altitudes (800 m, 3500 m and 6000 m, 3 levels). The Pentobarbital Sodium-induced sleeping test and the open field test were engaged to assess the effects of ESZ on sleeping time and spontaneous activity. Results (1)The drug and altitude had no interaction in the results of both the Pentobarbital Sodium-induced sleeping test and the open field test(P>0.05). (2)The time of Pentobarbital Sodium-induced sleeping of mice in the groups of ESZ at each altitudes were (37.77±18.22)min, (37.02±13.67)min, (95.67±47.68)min and in the groups of NS were(17.78±14.10)min, (15.09±12.46)min, (39.54±28.24)min respectively, and the sleep time in ESZ groups were significantly longer than those in the groups of NS (P 0.05); while the mice at the altitude of 6000 m in groups of ESZ and NS decreased compared with the groups at the altitude of 800 m after the relevant drugs intra-perineally for 6 h (P<0.05). Conclusion ESZ may prolong Pentobarbital Sodium-induced sleeping time especially at the altitude of 6000 m and with no influence on the spontaneous activity in mice exposed to acute hypobaric hypoxia.High altitude at 6000 m may prolong the sleep time induced by Pentobarbital Sodium and reduce the spontaneous activities. Key words: Eszopiclone; Hypobaric hypoxia; Sleeping time; Spontaneous activity
-
Effect of eszopiclone on Pentobarbital Sodium-induced sleeping time in acute hypobaric hypoxia mice
Chinese Journal of Behavioral Medicine and Brain Science, 2014Co-Authors: Ling Zhong, Yongbing Song, Jun Yang, Qian CaiAbstract:Objective To assess the effects of eszopiclone(ESZ) on the Pentobarbital Sodium-induced sleeping time and spontaneous activity in mice exposed to acute hypobaric hypoxia. Methods 120 mice were randomly divided into 6 groups by using two factors 2×3 levels factorial design, in which two factors were interventions (ESZ and 0.9% Sodium chloride, 2 levels) and altitudes (800 m, 3500 m and 6000 m, 3 levels). The Pentobarbital Sodium-induced sleeping test and the open field test were engaged to assess the effects of ESZ on sleeping time and spontaneous activity. Results (1)The drug and altitude had no interaction in the results of both the Pentobarbital Sodium-induced sleeping test and the open field test(P>0.05). (2)The time of Pentobarbital Sodium-induced sleeping of mice in the groups of ESZ at each altitudes were (37.77±18.22)min, (37.02±13.67)min, (95.67±47.68)min and in the groups of NS were(17.78±14.10)min, (15.09±12.46)min, (39.54±28.24)min respectively, and the sleep time in ESZ groups were significantly longer than those in the groups of NS (P 0.05); while the mice at the altitude of 6000 m in groups of ESZ and NS decreased compared with the groups at the altitude of 800 m after the relevant drugs intra-perineally for 6 h (P
Patricia E Burrows - One of the best experts on this subject based on the ideXlab platform.
-
comparison of oral Pentobarbital Sodium nembutal and oral chloral hydrate for sedation of infants during radiologic imaging preliminary results
American Journal of Roentgenology, 2003Co-Authors: Veronica Rooks, Taylor Chung, Linda Connor, David Zurakowski, Frederic A Hoffer, Keira P Mason, Patricia E BurrowsAbstract:OBJECTIVE. The purpose of this study was to compare the safety and efficacy of oral cherry-flavored Pentobarbital Sodium (Nembutal) and oral chloral hydrate to sedate infants undergoing radiologic imaging.SUBJECTS AND METHODS. We prospectively recorded data for all infants sedated with oral cherry-flavored Pentobarbital Sodium and oral chloral hydrate for imaging examinations between January 1997 and August 1999. The parameters recorded were each patient's age, weight, and American Society of Anesthesiologists classification; the time required to sedate; the total length of sedation time; the time required to discharge from the recovery room; and adverse events. The two-sample Student's t test and Fisher's exact test were used for statistical analysis.RESULTS. Oral Pentobarbital Sodium was administered to 317 infants. These infants had a mean age ± SD of 6.9 ± 3.1 months and a mean weight of 7.8 ± 4.8 kg; they received a median dose of 4 mg/kg of body weight. Oral chloral hydrate was administered to 358 i...