The Experts below are selected from a list of 252 Experts worldwide ranked by ideXlab platform
Václav Kašička - One of the best experts on this subject based on the ideXlab platform.
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Capillary electrophoresis-mass spectrometry for Peptide Analysis: target-based approaches and proteomics/peptidomics strategies.
Methods in molecular biology (Clifton N.J.), 2013Co-Authors: Carolina Simó, Alejandro Cifuentes, Václav KašičkaAbstract:In this chapter, the potential of capillary electrophoresis-mass spectrometry (CE-MS) for Peptide Analysis is demonstrated by the presentation of two different strategies typically followed in Analysis of these biomolecules by CE-MS. The first one is a target-based approach and it is used to detect a toxic oligoPeptide in a complex matrix. Namely, CE-MS using an ion trap MS analyzer is applied to detect and quantify γ-glutamyl-S-ethenyl-cysteine (GEC) bioactive diPeptide in a legume plant. The second one is a shotgun-like methodology used for proteomic Analysis. Particularly, CE-MS using a time-of-flight MS analyzer is employed to investigate the substantial equivalence between a genetically modified (GM) variety of soybean and its conventional isogenic counterpart. These generic methods have broad applications for the Analysis of Peptides in a large variety of matrices, including applications in the area of proteomics and peptidomics.
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Conversion of capillary zone electrophoresis to free-flow zone electrophoresis using a simple model of their correlation : application to synthetic enkephalin-type Peptide Analysis and preparation
Journal of Chromatography A, 1992Co-Authors: Václav Kašička, Zdenĕk Prusik, Jan PospíšekAbstract:Abstract A simple mathematical model of the correlation between capillary zone electrophoresis (CZE) and continuous free-flow zone electrophoresis (FFZE) was developed. The model results from the fact that both methods are based on the same separation principle (zone electrophoresis) and both are performed in a free solution of the same composition. Based on this model, a procedure for the conversion of an analytical microscale CZE separation into a preparative FFZE separation was developed. The applicability of this procedure is demonstrated by CZE Analysis at the picomole level and FFZE preparative fractionation (60 mg/h throughput) of crude synthetic biologically active Peptide [ d -Tle2,5]-dalargin. The combination of CZE and FFZE provides an efficient and economical tool for synthetic Peptide Analysis and preparation.
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Application of capillary isotachophoresis in Peptide Analysis.
Journal of chromatography, 1991Co-Authors: Václav Kašička, Zdeněk PrusíkAbstract:Abstract This paper gives a broad and detailed review of the applications of one of the modern high-performance electromigration separation techniques — capillary isotachophoresis (ITP) — in Peptide Analysis. Examples are presented of the utilization of capillary ITP for Peptide Analysis in the fields of chemistry, general and clinical biochemistry, biology, biotechnology, pharmacy and the food industry. The complete composition of all the electrolyte systems used for Peptide ITP analyses in both cationic and anionic techniques is given in tabular form. According to the purpose of Analysis the applications are divided into several sections: model studies, determination of physico-chemical characteristics, purity control of both intermediate and final Peptide preparations, including the determination of low-molecular-mass ionogenic admixtures, and the Analysis of Peptides in biological fluids and tissue extracts. In addition to the main applications the theoretical and methodological aspects of Peptide ITP Analysis are discussed. The basic electromigration properties of Peptides (their polyampholyte character, effective and absolute mobilities, acid—base equilibria) are explained and the selection of parameters for Peptide ITP Analysis is described in detail. The advantages and disadvantages of ITP compared with other electrophoretic and chromatographic methods used for Peptide Analysis are discussed.
Hans Jörnvall - One of the best experts on this subject based on the ideXlab platform.
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Microfluidic systems and proteomics: applications of the electrocapture technology to protein and Peptide Analysis.
Analytical biochemistry, 2005Co-Authors: Juan Astorga-wells, Susanne Vollmer, Tomas Bergman, Hans JörnvallAbstract:Microanalytical systems have been visualized as thenext evolutionary step in analytical instrumentation. Indownscaling methodologies, lower sample and reagentconsumption, easier automation, higher throughput, andsimpler integration have been envisioned [1–3]. Thesedevices promise to impact any discipline where a chemi-cal Analysis is needed, e.g., in diagnostics and clinicalprocedures [4,5], in vivo sensors, quality control ofindustrial processes [6], high-throughput screening [7,8],and in places where on-site Analysis is needed, such as inenvironmental monitoring [9], chemical warfare [10], andextraterrestrial Analysis [11]. With these characteristics,microXuidic systems are of particular interest in proteinand Peptide Analysis [12–14]. This review presents theelectrocapture technology as a microXuidic tool in pro-tein and Peptide Analysis.The Analysis of proteins has been continuously facil-itated because of advances in genomics, bioinformatics,and analytical tools, especially in mass spectrometry[15–17]. Although progress has shortened the Analysistime by many orders of magnitude (about 10
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Thematic Review: New analytical techniques in proteomics MicroXuidic systems and proteomics: Applications of the electrocapture technology to protein and Peptide Analysis
2005Co-Authors: Juan Astorga-wells, Susanne Vollmer, Tomas Bergman, Hans JörnvallAbstract:Microanalytical systems have been visualized as thenext evolutionary step in analytical instrumentation. Indownscaling methodologies, lower sample and reagentconsumption, easier automation, higher throughput, andsimpler integration have been envisioned [1–3]. Thesedevices promise to impact any discipline where a chemi-cal Analysis is needed, e.g., in diagnostics and clinicalprocedures [4,5], in vivo sensors, quality control ofindustrial processes [6], high-throughput screening [7,8],and in places where on-site Analysis is needed, such as inenvironmental monitoring [9], chemical warfare [10], andextraterrestrial Analysis [11]. With these characteristics,microXuidic systems are of particular interest in proteinand Peptide Analysis [12–14]. This review presents theelectrocapture technology as a microXuidic tool in pro-tein and Peptide Analysis.The Analysis of proteins has been continuously facil-itated because of advances in genomics, bioinformatics,and analytical tools, especially in mass spectrometry[15–17]. Although progress has shortened the Analysistime by many orders of magnitude (about 10
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Purification and characterization of a Kunitz-type trypsin inhibitor from Leaf-nosed viper venom.
FEBS letters, 1991Co-Authors: Abdur Rehman Siddiqi, Zafar H. Zaidi, Hans JörnvallAbstract:A Kunitz-type trypsin inhibitor was purified from Leaf-nosed viper venom and the primary structure determined by Peptide Analysis. In relation to other trypsin inhibitors, the protein has an extended C-terminal segment and a distinct pattern of residue alterations at the functionally important contact sites with proteases.
Peter Højrup - One of the best experts on this subject based on the ideXlab platform.
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Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis
Methods in molecular biology (Clifton N.J.), 2015Co-Authors: Karin Hjernø, Peter HøjrupAbstract:The aim of this chapter is to give a short introduction to Peptide Analysis by mass spectrometry (MS) and interpretation of fragment mass spectra. Through examples and guidelines we demonstrate how to understand and validate search results and how to perform de novo sequencing based on the often very complex fragmentation pattern obtained by tandem mass spectrometry (also referred to as MSMS). The focus is on simple rules for interpretation of MSMS spectra of tryptic as well as non-tryptic Peptides.
Alejandro Cifuentes - One of the best experts on this subject based on the ideXlab platform.
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Capillary electrophoresis-mass spectrometry for Peptide Analysis: target-based approaches and proteomics/peptidomics strategies.
Methods in molecular biology (Clifton N.J.), 2013Co-Authors: Carolina Simó, Alejandro Cifuentes, Václav KašičkaAbstract:In this chapter, the potential of capillary electrophoresis-mass spectrometry (CE-MS) for Peptide Analysis is demonstrated by the presentation of two different strategies typically followed in Analysis of these biomolecules by CE-MS. The first one is a target-based approach and it is used to detect a toxic oligoPeptide in a complex matrix. Namely, CE-MS using an ion trap MS analyzer is applied to detect and quantify γ-glutamyl-S-ethenyl-cysteine (GEC) bioactive diPeptide in a legume plant. The second one is a shotgun-like methodology used for proteomic Analysis. Particularly, CE-MS using a time-of-flight MS analyzer is employed to investigate the substantial equivalence between a genetically modified (GM) variety of soybean and its conventional isogenic counterpart. These generic methods have broad applications for the Analysis of Peptides in a large variety of matrices, including applications in the area of proteomics and peptidomics.
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Capillary electrophoresis-electrospray-mass spectrometry in Peptide Analysis and peptidomics
Electrophoresis, 2008Co-Authors: Miguel Herrero, Elena Ibáñez, Alejandro CifuentesAbstract:In the present work, an exhaustive review of the main developments and applications of CE-MS for Peptide Analysis is given. This review includes the use of different CE separation modes, MS analyzers, capillary coatings, preconcentration techniques, on-chip applications as well as other different multidimensional strategies for Peptide Analysis. Key applications are critically discussed and relevant works published from January 2000 to May 2007 are summarized including information concerning the type of sample, CE-MS parameters as well as some figures of merit of the different CE-MS procedures developed for Peptide Analysis and peptidomics.
Jan Pospíšek - One of the best experts on this subject based on the ideXlab platform.
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Conversion of capillary zone electrophoresis to free-flow zone electrophoresis using a simple model of their correlation : application to synthetic enkephalin-type Peptide Analysis and preparation
Journal of Chromatography A, 1992Co-Authors: Václav Kašička, Zdenĕk Prusik, Jan PospíšekAbstract:Abstract A simple mathematical model of the correlation between capillary zone electrophoresis (CZE) and continuous free-flow zone electrophoresis (FFZE) was developed. The model results from the fact that both methods are based on the same separation principle (zone electrophoresis) and both are performed in a free solution of the same composition. Based on this model, a procedure for the conversion of an analytical microscale CZE separation into a preparative FFZE separation was developed. The applicability of this procedure is demonstrated by CZE Analysis at the picomole level and FFZE preparative fractionation (60 mg/h throughput) of crude synthetic biologically active Peptide [ d -Tle2,5]-dalargin. The combination of CZE and FFZE provides an efficient and economical tool for synthetic Peptide Analysis and preparation.