The Experts below are selected from a list of 2235 Experts worldwide ranked by ideXlab platform
Liu Congrong - One of the best experts on this subject based on the ideXlab platform.
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Alveolar soft part sarcoma of uterine cervix in a postmenopausal woman: a case report and review of literature
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2017Co-Authors: Zhang Hongyan, Wang Yuxiang, Liu Yan, Liu CongrongAbstract:We report a case of alveolar soft part sarcoma (ASPS) in a 68-year-old woman's uterine cervix. Macroscopic observation revealed an unencapsulated lesion confined to the cervical stroma, measuring 10 mm in diameter. Microscopic examination showed large, round or polygonal tumor cells, arranging in a well-defined nests pattern with scanty vascular stroma. Immunohistochemistry was performed. Tumor cells showed positivity for Ki-67 (2%), TFE-3, CD68, and myoD1 (cytoplasm), while negative for HNF1 beta, CD10, RCC, PAX-8, CK7, CK18, CK8, CR, EMA, HMB-45, S-100, CgA, and Syn. Periodic Acid-Schiff Stain after diastase digestion (PAS-D) had revealed rod-like or rhomboid crystals of some cells. The diagnosis of ASPS was confirmed. ASPS was a rare soft tissue malignancy in female reproductive system. After a detailed study of the reported cases, we summarized some clinicopathological differences between cervical and soft tissue ASPS.National Natural Science Foundation of China [81472430]; National Key Scientific Instru-ments and Equipment Development Program of China [2013YQ03065108]SCI(E)ARTICLE99812-98151
Z. L. Bouzon - One of the best experts on this subject based on the ideXlab platform.
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Effect of ultraviolet-B radiation on growth, photosynthetic pigments, and cell biology of Kappaphycus alvarezii (Rhodophyta, Gigartinales) macroalgae brown strain
Photosynthetica, 2010Co-Authors: É. C. Schmidt, B. G. Nunes, M. Maraschin, Z. L. BouzonAbstract:Kappaphycus alvarezii is a seaweed of great economic importance for the extraction of kappa carrageenan from its cell walls. The most common strains are dark red, brown, yellow, and different gradations of green. It is known that ultraviolet radiation (UVR) affects macroalgae in many important ways, including reduced growth rate, reduction of primary productivity, and changes in cell biology and ultrastructure. Therefore, we examined the brown strain of K. alvarezii exposed to ultraviolet-B radiaton (UVBR) for 3 h per day during 28 days of cultivation. The control plants showed growth rates of 7.27% d^−1, while plants exposed to UVBR grew only 4.0% d^−1. Significant differences in growth rates and in phycobiliproteins between control and exposed plants were also found. Compared with control plants, phycobiliprotein contents were observed to decrease after UV-B exposure. Furthermore, the chlorophyll a (Chl a ) contents decreased and showed significant differences. UVBR also caused changes in the ultrastructure of cortical and subcortical cells, which included increased thickness of the cell wall and number of plastoglobuli, reduced intracellular spaces, changes in the cell contour, and destruction of chloroplast internal organization. Reaction with Toluidine Blue showed an increase in the thickness of the cell wall, and Periodic Acid-Schiff Stain showed a decrease in the number of starch grains. By the significant changes in growth rates, photosynthetic contents and ultrastructual changes observed, it is clear that UVBR negatively affects intertidal macroalgae and, by extension, their economic viability.
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changes in ultrastructure and histochemistry of two red macroalgae strains of kappaphycus alvarezii rhodophyta gigartinales as a consequence of ultraviolet b radiation exposure
Micron, 2009Co-Authors: É. C. Schmidt, Lidiane Angela Scariot, Ticiane Rover, Z. L. BouzonAbstract:Ultraviolet radiation (UVR) affects macroalgae in many important ways, including reduced growth rate, reduction of primary productivity and changes in cell biology and ultrastructure. Among red macroalgae, Kappaphycus alvarezii is of economic interest by its production of kappa carrageenan. Only a few reports have examined the changes in macroalgae ultrastructure and cell biology resulting from UVB radiation exposure. Therefore, we examined two strains of K. alvarezii (green and red) exposed to UVB for 3 h per day during 28 days and then processed them for histochemical and electron microscopy analysis. Reaction with Toluidine Blue showed an increase in the thickness of the cell wall and Periodic Acid-Schiff Stain showed a decrease in the number of starch grains. UVBR also caused changes in the ultrastructure of cortical and subcortical cells, which included increased thickness of the cell wall and number of free ribosomes and plastoglobuli, reduced intracellular spaces, changes in the cell contour, and destruction of chloroplast internal organization. Based on these lines of evidence, it was evident by the ultrastructural changes observed that UVBR negatively affects intertidal macroalgae and, by extension, their economic viability.
Abdul Razak Tariq - One of the best experts on this subject based on the ideXlab platform.
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Fundamental basis of nephropathy in chronic low dose organophosphates exposure
2019Co-Authors: Abdul Razak Tariq, Abdullah, Nor Zamzila, A.talib Norlelawati, Aung Sanda, Wan Salleh, Wan Muhamad SalahudinAbstract:Objectives: People are exposed to chronic low dose organophosphates (OPs) pesticides through agriculture and pest control activities. Apparently, there were significant pidemiological reports suggesting pesticides role in chronic kidney disease. This observation is yet to be confirmed with appropriate experimental model of chronic OPs exposure on animals. Thus, the aim of this study is to observe for histomorphological characteristics related to nephropathy in the kidneys of rats upon exposure to chronic low dose OPs. Materials and Methods: 24 males Sprague-Dawley rats were randomly divided into 3 groups of 6 rats each with Group 1 as a Control group, whilst Group 2 and Group 3 received subcutaneous vehicle (3% dimethyl sulphoxide + 97% v/v soy oil) and 18.0 mg/kg BW chlorpyrifos respectively, every other day for 180 days. After treatment, the rats were sacrificed and the right kidney was subjected to routine H&E examination and specific Masson-Trichrome and Periodic-acid Schiff Stain. Results: OPs exposed group (Group 3) exhibited significant histo-morphological abnormalities suggesting cell injury, apoptosis and degenerative changes in glomeruli and tubules. In contrast, the control groups 1 and 2 (vehicle) are spared. Conclusion: Chronic low dose OPs exposure induced nephrotoxicity. The exact mechanism of nephrotoxicity should be further investigated
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Nephrotoxicity in chronic low dose organophosphate exposure
2016Co-Authors: Aung Sanda, Abdullah, Nor Zamzila, A.talib Norlelawati, Mohd Noor Noriah, Harun Norra, Zainone, Zamzuria Mat, Abdul Razak TariqAbstract:Objectives: People are exposed to chronic low dose organophosphates (OPs) pesticides through agriculture and pest control activities. Apparently, there were significant epidemiological reports suggesting pesticides role in chronic kidney disease. This observation is yet to be confirmed with appropriate experimental model of chronic OPs exposure on animals. Thus, the aim of this study is to observe for histomorphological characteristics related to nephropathy in the kidneys of rats upon exposure to chronic low dose OPs. Materials and Methods: 24 males Sprague-Dawley rats were randomly divided into 3 groups of 6 rats each with Group 1 as a Control group, whilst Group 2 and Group 3 received subcutaneous vehicle (3% dimethyl sulphoxide + 97% v/v soy oil) and 18.0 mg/kg BW chlorpyrifos respectively, every other day for 180 days. After treatment, the rats were sacrificed and the right kidney was subjected to routine H&E examination and specific Masson-Trichrome and Periodic-acid Schiff Stain. Results: OPs exposed group (Group 3) exhibited significant histo-morphological abnormalities suggesting cell injury, apoptosis and degenerative changes in glomeruli and tubules. In contrast, the control groups 1 and 2 (vehicle) are spared. Conclusion: Chronic low dose OPs exposure induced nephrotoxicity. The exact mechanism of nephrotoxicity should be further investigated. Keywords
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Nephrotoxicity in chronic low dose organophosphate exposure
College of Pathologists Academy of Medicine Malaysia, 2016Co-Authors: Aung Sanda, Abdullah, Nor Zamzila, A.talib Norlelawati, Mohd Noor Noriah, Harun Norra, Mat Zainone Zamzuria, Abdul Razak TariqAbstract:Objectives: People are exposed to chronic low dose organophosphates (OPs) pesticides through agriculture and pest control activities. Apparently, there were signifcant epidemiological reports suggesting pesticides role in chronic kidney disease. This observation is yet to be confrmed with appropriate experimental model of chronic OPs exposure on animals. Thus, the aim of this study is to observe for histomorphological characteristics related to nephropathy in the kidneys of rats upon exposure to chronic low dose OPs. Materials and Methods: 24 males Sprague-Dawley rats were randomly divided into 3 groups of 6 rats each with Group 1 as a Control group, whilst Group 2 and Group 3 received subcutaneous vehicle (3% dimethyl sulphoxide + 97% v/v soy oil) and 18.0 mg/kg BW chlorpyrifos respectively, every other day for 180 days. After treatment, the rats were sacrifced and the right kidney was subjected to routine H&E examination and specifc Masson-Trichrome and Periodic-acid Schiff Stain. Results: OPs exposed group (Group 3) exhibited signifcant histo-morphological abnormalities suggesting cell injury, apoptosis and degenerative changes in glomeruli and tubules. In contrast, the control groups 1 and 2 (vehicle) are spared. Conclusion: Chronic low dose OPs exposure induced nephrotoxicity. The exact mechanism of nephrotoxicity should be further investigated
Zhang Hongyan - One of the best experts on this subject based on the ideXlab platform.
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Alveolar soft part sarcoma of uterine cervix in a postmenopausal woman: a case report and review of literature
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2017Co-Authors: Zhang Hongyan, Wang Yuxiang, Liu Yan, Liu CongrongAbstract:We report a case of alveolar soft part sarcoma (ASPS) in a 68-year-old woman's uterine cervix. Macroscopic observation revealed an unencapsulated lesion confined to the cervical stroma, measuring 10 mm in diameter. Microscopic examination showed large, round or polygonal tumor cells, arranging in a well-defined nests pattern with scanty vascular stroma. Immunohistochemistry was performed. Tumor cells showed positivity for Ki-67 (2%), TFE-3, CD68, and myoD1 (cytoplasm), while negative for HNF1 beta, CD10, RCC, PAX-8, CK7, CK18, CK8, CR, EMA, HMB-45, S-100, CgA, and Syn. Periodic Acid-Schiff Stain after diastase digestion (PAS-D) had revealed rod-like or rhomboid crystals of some cells. The diagnosis of ASPS was confirmed. ASPS was a rare soft tissue malignancy in female reproductive system. After a detailed study of the reported cases, we summarized some clinicopathological differences between cervical and soft tissue ASPS.National Natural Science Foundation of China [81472430]; National Key Scientific Instru-ments and Equipment Development Program of China [2013YQ03065108]SCI(E)ARTICLE99812-98151
É. C. Schmidt - One of the best experts on this subject based on the ideXlab platform.
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responses of the macroalgae hypnea musciformis after in vitro exposure to uv b
Aquatic Botany, 2012Co-Authors: É. C. Schmidt, Beatriz Pereira, Rodrigo Weber Dos Santos, Claudiane Gouveia, Giulia Burle Costa, Gabriel S M Faria, Fernando Scherner, Paulo Antunes Horta, Roberta De Paula Martins, Alexandra LatiniAbstract:Abstract The in vitro effects of UVBR were investigated in apical segments of Hypnea musciformis. The plants were cultivated and exposed to photosynthetically active radiation (PAR) at 80 μmol photons m−2 s−1 and PAR + UVBR at 1.6 W m−2 at 3 h per day for 7 days. Toluidine Blue reaction showed metachromatic granulations in vacuole, and Periodic Acid Schiff Stain showed a decrease in the number of floridean starch grains. UVBR also caused changes in the ultrastructure of cortical cells, which included increased thickness of the cell wall, reduced intracellular spaces, changes in the cell contour, destruction of chloroplast internal organization, and rough endoplasmic reticula increase. The algae cultivated under PAR-only showed growth rates of 9.7% day−1, while algae exposed to PAR + UVBR grew only 3.2% day−1. Furthermore, compared with control algae, phycobiliprotein contents (phycoerythrin, phycocyanin, and allophycocyanin) were observed to decrease after PAR + UVBR. However, chlorophyll a levels were not significantly different (ANOVA, P = 0.52) after exposure to PAR + UVBR. As a photoprotective adaptation strategy against UVB damage, an increase of 58.9% phenolic compounds and 3.6% of carotenoids was observed. Overall, these results lead to the conclusion that both ultrastructural damage and observable changes in metabolism occurred in H. musciformis after only 3 h of daily UVB exposure over a 7-d experimental period.
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Effect of ultraviolet-B radiation on growth, photosynthetic pigments, and cell biology of Kappaphycus alvarezii (Rhodophyta, Gigartinales) macroalgae brown strain
Photosynthetica, 2010Co-Authors: É. C. Schmidt, B. G. Nunes, M. Maraschin, Z. L. BouzonAbstract:Kappaphycus alvarezii is a seaweed of great economic importance for the extraction of kappa carrageenan from its cell walls. The most common strains are dark red, brown, yellow, and different gradations of green. It is known that ultraviolet radiation (UVR) affects macroalgae in many important ways, including reduced growth rate, reduction of primary productivity, and changes in cell biology and ultrastructure. Therefore, we examined the brown strain of K. alvarezii exposed to ultraviolet-B radiaton (UVBR) for 3 h per day during 28 days of cultivation. The control plants showed growth rates of 7.27% d^−1, while plants exposed to UVBR grew only 4.0% d^−1. Significant differences in growth rates and in phycobiliproteins between control and exposed plants were also found. Compared with control plants, phycobiliprotein contents were observed to decrease after UV-B exposure. Furthermore, the chlorophyll a (Chl a ) contents decreased and showed significant differences. UVBR also caused changes in the ultrastructure of cortical and subcortical cells, which included increased thickness of the cell wall and number of plastoglobuli, reduced intracellular spaces, changes in the cell contour, and destruction of chloroplast internal organization. Reaction with Toluidine Blue showed an increase in the thickness of the cell wall, and Periodic Acid-Schiff Stain showed a decrease in the number of starch grains. By the significant changes in growth rates, photosynthetic contents and ultrastructual changes observed, it is clear that UVBR negatively affects intertidal macroalgae and, by extension, their economic viability.
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changes in ultrastructure and histochemistry of two red macroalgae strains of kappaphycus alvarezii rhodophyta gigartinales as a consequence of ultraviolet b radiation exposure
Micron, 2009Co-Authors: É. C. Schmidt, Lidiane Angela Scariot, Ticiane Rover, Z. L. BouzonAbstract:Ultraviolet radiation (UVR) affects macroalgae in many important ways, including reduced growth rate, reduction of primary productivity and changes in cell biology and ultrastructure. Among red macroalgae, Kappaphycus alvarezii is of economic interest by its production of kappa carrageenan. Only a few reports have examined the changes in macroalgae ultrastructure and cell biology resulting from UVB radiation exposure. Therefore, we examined two strains of K. alvarezii (green and red) exposed to UVB for 3 h per day during 28 days and then processed them for histochemical and electron microscopy analysis. Reaction with Toluidine Blue showed an increase in the thickness of the cell wall and Periodic Acid-Schiff Stain showed a decrease in the number of starch grains. UVBR also caused changes in the ultrastructure of cortical and subcortical cells, which included increased thickness of the cell wall and number of free ribosomes and plastoglobuli, reduced intracellular spaces, changes in the cell contour, and destruction of chloroplast internal organization. Based on these lines of evidence, it was evident by the ultrastructural changes observed that UVBR negatively affects intertidal macroalgae and, by extension, their economic viability.