The Experts below are selected from a list of 16215 Experts worldwide ranked by ideXlab platform
Saso Ivanovski - One of the best experts on this subject based on the ideXlab platform.
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a biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone Periodontal Ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:Abstract This study describes the design of a biphasic scaffold composed of a Fused Deposition Modeling scaffold (bone compartment) and an electrospun membrane (Periodontal compartment) for Periodontal regeneration. In order to achieve simultaneous alveolar bone and Periodontal Ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple Periodontal Ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, Periodontal Ligament and cementum.
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a biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone Periodontal Ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Wei Fan, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the design of a biphasic scaffold composed of a Fused Deposition Modeling scaffold (bone compartment) and an electrospun membrane (Periodontal compartment) for Periodontal regeneration. In order to achieve simultaneous alveolar bone and Periodontal Ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple Periodontal Ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, Periodontal Ligament and cementum. © 2012 Elsevier Ltd.
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a biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone Periodontal Ligament complex
Institute of Health and Biomedical Innovation; Science & Engineering Faculty, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the design of a biphasic scaffold composed of a Fused Deposition Modeling scaffold (bone compartment) and an electrospun membrane (Periodontal compartment) for Periodontal regeneration. In order to achieve simultaneous alveolar bone and Periodontal Ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple Periodontal Ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, Periodontal Ligament and cementum. © 2012 Elsevier Ltd.
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The effect of platelet-rich plasma on osteoblast and Periodontal Ligament cell migration, proliferation and differentiation.
Journal of Periodontal Research, 2009Co-Authors: F. Creeper, R. I. Marshall, Agnieszka M. Lichanska, Gregory J. Seymour, Saso IvanovskiAbstract:BACKGROUND AND OBJECTIVE: Platelet-rich plasma is used to deliver growth factors, in a safe and convenient manner, for enhancing bone and Periodontal regeneration. However, conflicting reports regarding its effectiveness suggest that further study of the relevant cellular mechanisms is required. The aim of this study was to investigate the in vitro effect of platelet-rich plasma on osteoblasts and Periodontal Ligament cell function. MATERIAL AND METHODS: Various concentrations of platelet-rich plasma (100, 50, 20 and 10%) and platelet-poor plasma, obtained from human donors, were applied to primary cultures of human osteoblasts and Periodontal Ligament cells. [(3)H]-Thymidine incorporation, crystal violet staining and MTT assays were utilized to assess DNA synthesis and proliferation. Migration was determined by assessing the cell response to a concentration gradient, while differentiation was assessed using Alazarin Red staining. RESULTS: Platelet-rich plasma and platelet-poor plasma had stimulatory effects on the migration of both human osteoblasts and Periodontal Ligament cells. At 24 h, DNA synthesis was suppressed by the application of the various concentrations of platelet-rich plasma, but over a 5-d period, a beneficial effect on proliferation was observed, especially in response to 50% platelet-rich plasma. Platelet-poor plasma resulted in the greatest enhancement of cellular proliferation for both cell types. At a concentration of 50%, platelet-rich plasma and platelet-poor plasma facilitated differentiation of both cell types. CONCLUSION: Platelet-rich plasma can exert a positive effect on osteoblast and Periodontal Ligament cell function, but this effect is concentration specific with maximal concentrations not necessarily resulting in optimal outcomes. Platelet-poor plasma also appears to have the ability to promote wound healing-associated cell function.
Zhenhua Yang - One of the best experts on this subject based on the ideXlab platform.
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Periodontal tissue engineering with stem cells from the Periodontal Ligament of human retained deciduous teeth
Journal of Periodontal Research, 2013Co-Authors: Yanli Liu, Zhenhua Yang, Fusheng Yang, Xi Wang, Ku XuaAbstract:BACKGROUND AND OBJECTIVE: Periodontal Ligament stem cells from human permanent teeth (PePDLSCs) have been investigated extensively in Periodontal tissue engineering and regeneration. However, little knowledge is available on the Periodontal Ligament stem cells from human retained deciduous teeth (DePDLSCs). This study evaluated the potential of DePDLSCs in Periodontal tissue regeneration. MATERIAL AND METHODS: DePDLSCs were isolated and purified by limited dilution. The characteristics of DePDLSCs were evaluated and compared with PePDLSCs both in vitro and in vivo. RESULTS: DePDLSCs presented a higher proliferation rate and colony-forming capacity than PePDLSCs in vitro. During the osteogenic induction, alkaline phosphatase (ALP) activity, mineralized matrix formation and expression of mineralization-related genes, including runt-related transcription factor 2 (RUNX2), ALP, collagen type I (COLI) and osteocalcin (OCN) were significantly enhanced in DePDLSCs compared with PePDLSCs. Furthermore, DePDLSC cell sheets showed a stronger synthesis of collagen type I in the extracellular matrix than did PePDLSC cell sheets. After in vivo transplantation, DePDLSC cell sheets recombined with human dentin blocks were able to generate new cementum/Periodontal Ligament-like tissues. CONCLUSION: Our findings suggest that DePDLSCs can be used as a promising candidate for Periodontal tissue engineering.
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Periodontal tissue engineering with stem cells from the Periodontal Ligament of human retained deciduous teeth
Journal of Periodontal Research, 2013Co-Authors: Yanli Liu, Zhenhua Yang, Fusheng Yang, Xi Wang, Lingying Wen, Kun XuanAbstract:Abstract Ji K, Liu Y, Lu W, Yang F, Yu J, Wang X, Ma Q, Yang Z, Wen L, Xuan K. Periodontal tissue engineering with stem cells from the Periodontal Ligament of human retained deciduous teeth. J Periodont Res 2012; doi: 10.1111/j.1600-0765.2012.01509.x. © 2012 John Wiley a Sons A/S Background and Objective: Periodontal Ligament stem cells from human permanent teeth (PePDLSCs) have been investigated extensively in Periodontal tissue engineering and regeneration. However, little knowledge is available on the Periodontal Ligament stem cells from human retained deciduous teeth (DePDLSCs). This study evaluated the potential of DePDLSCs in Periodontal tissue regeneration. Material and Methods: DePDLSCs were isolated and purified by limited dilution. The characteristics of DePDLSCs were evaluated and compared with PePDLSCs both in vitro and in vivo. Results: DePDLSCs presented a higher proliferation rate and colony-forming capacity than PePDLSCs in vitro. During the osteogenic induction, alkaline phosphatase (ALP) activity, mineralized matrix formation and expression of mineralization-related genes, including runt-related transcription factor 2 (RUNX2), ALP, collagen type I (COLI) and osteocalcin (OCN) were significantly enhanced in DePDLSCs compared with PePDLSCs. Furthermore, DePDLSC cell sheets showed a stronger synthesis of collagen type I in the extracellular matrix than did PePDLSC cell sheets. After in vivo transplantation, DePDLSC cell sheets recombined with human dentin blocks were able to generate new cementum/Periodontal Ligament-like tissues. Conclusion: Our findings suggest that DePDLSCs can be used as a promising candidate for Periodontal tissue engineering.
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tissue engineering of cementum Periodontal Ligament complex using a novel three dimensional pellet cultivation system for human Periodontal Ligament stem cells
Tissue Engineering Part C-methods, 2009Co-Authors: Zhenhua Yang, Z Lin, Fang Jin, Xiaojun Zhang, Chun Han, Na Huo, Yinxiong Wang, Yunfei Zhang, Yan JinAbstract:Limitations of conventional regeneration modalities underscore the necessity of recapitulating development for Periodontal tissue engineering. In this study, we proposed a novel three-dimensional pellet cultivation system for Periodontal Ligament stem cells (PDLSCs) to recreate the biological microenvironment similar to those of a regenerative milieu. Monodispersed human PDLSCs were cultured in medium with ascorbic acid and conditioned medium from developing apical tooth germ cells and were subsequently harvested from culture plate as a contiguous cell sheet with abundant extracellular matrix. The detached cell–matrix membrane spontaneously contracted to produce a single-cell pellet. The PDLSCs embedded within this cell–matrix complex exhibited several phenotypic characteristics of cementoblast lineages, as indicated by upregulated alkaline phosphatase activity, accelerated mineralization, and the expression of bone sialoprotein and osteocalcin genes. When this PDLSC pellets were transplanted into immunoc...
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apical tooth germ cell conditioned medium enhances the differentiation of Periodontal Ligament stem cells into cementum Periodontal Ligament like tissues
Journal of Periodontal Research, 2009Co-Authors: Zhenhua Yang, X J Zhang, N N Dang, Z F, Y J Sun, Yinzhong Duan, Z Lin, Yan JinAbstract:Background and Objective: Limitations of current Periodontal regeneration modalities in both predictability and extent of healing response, especially on new cementum and attachment formation, underscore the importance of restoring or providing a microenvironment that is capable of promoting the differentiatiation of Periodontal Ligament stem cells (PDLSCs) towards cementoblast-like cells and the formation of cementum/Periodontal Ligament-like tissues. The aim of this study was to investigate the biological effect of conditioned medium from developing apical tooth germ cells (APTG-CM) on the differentiation and cementogenesis of PDLSCs both in vitro and in vivo. Material and Methods: Using the limiting dilution technique, single-colony-derived human PDLSCs were isolated and expanded to obtain homogeneous populations of PDLSCs. Morphological appearance, cell cycle analysis, bromodeoxyuridine incorporation, alkaline phosphatase (ALP) activity, mineralization behavior, gene expression of cementoblast phenotype and in vivo differentiation capacities of PDLSCs co-cultured with APTG-CM were evaluated. Results: The induced PDLSCs exhibited several characteristics of cementoblast lineages, as indicated by the morphological changes, increased proliferation, high ALP activity, and the expression of cementum-related genes and calcified nodule formation in vitro. When transplanted into immunocompromised mice, the induced PDLSCs showed tissue-regenerative capacity to produce cementum/Periodontal Ligament-like structures, characterized by a layer of cementum-like mineralized tissues and associated Periodontal Ligament-like collagen fibers connecting with the newly formed cementum-like deposits, whereas control, untreated PDLSCs transplants mainly formed connective tissues. Conclusion: Our findings suggest that APTG-CM is able to provide a cementogenic microenvironment and induce differentiation of PDLSCs along the cementoblastic lineage. This has important implications for Periodontal engineering.
Yan Jin - One of the best experts on this subject based on the ideXlab platform.
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jawbone microenvironment promotes periodontium regeneration by regulating the function of Periodontal Ligament stem cells
Scientific Reports, 2017Co-Authors: Bin Zhu, Wenjia Liu, Yihan Liu, Xicong Zhao, Hao Zhang, Zhuojing Luo, Yan JinAbstract:During tooth development, the jawbone interacts with dental germ and provides the development microenvironment. Jawbone-derived mesenchymal stem cells (JBMSCs) maintain this microenvironment for root and periodontium development. However, the effect of the jawbone microenvironment on periodontium tissue regeneration is largely elusive. Our previous study showed that cell aggregates (CAs) of bone marrow mesenchymal stem cells promoted periodontium regeneration on the treated dentin scaffold. Here, we found that JBMSCs enhanced not only the osteogenic differentiation of Periodontal Ligament stem cells (PDLSCs) but also their adhesion to titanium (Ti) material surface. Importantly, the compound CAs of PDLSCs and JBMSCs regenerated Periodontal Ligament-like fibers and mineralized matrix on the Ti scaffold surface, both in nude mice ectopic and minipig orthotopic transplantations. Our data revealed that an effective regenerative microenvironment, reconstructed by JBMSCs, promoted periodontium regeneration by regulating PDLSCs function on the Ti material.
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treatment of Periodontal intrabony defects using autologous Periodontal Ligament stem cells a randomized clinical trial
Stem Cell Research & Therapy, 2016Co-Authors: Faming Chen, Lina Gao, Beimin Tian, Xiyu Zhang, Yongjie Zhang, Guangying Dong, Qing Chu, Yuan Yin, Songtao Shi, Yan JinAbstract:Background Periodontitis, which progressively destroys tooth-supporting structures, is one of the most widespread infectious diseases and the leading cause of tooth loss in adults. Evidence from preclinical trials and small-scale pilot clinical studies indicates that stem cells derived from Periodontal Ligament tissues are a promising therapy for the regeneration of lost/damaged Periodontal tissue. This study assessed the safety and feasibility of using autologous Periodontal Ligament stem cells (PDLSCs) as an adjuvant to grafting materials in guided tissue regeneration (GTR) to treat Periodontal intrabony defects. Our data provide primary clinical evidence for the efficacy of cell transplantation in regenerative dentistry.
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tissue engineering of cementum Periodontal Ligament complex using a novel three dimensional pellet cultivation system for human Periodontal Ligament stem cells
Tissue Engineering Part C-methods, 2009Co-Authors: Zhenhua Yang, Z Lin, Fang Jin, Xiaojun Zhang, Chun Han, Na Huo, Yinxiong Wang, Yunfei Zhang, Yan JinAbstract:Limitations of conventional regeneration modalities underscore the necessity of recapitulating development for Periodontal tissue engineering. In this study, we proposed a novel three-dimensional pellet cultivation system for Periodontal Ligament stem cells (PDLSCs) to recreate the biological microenvironment similar to those of a regenerative milieu. Monodispersed human PDLSCs were cultured in medium with ascorbic acid and conditioned medium from developing apical tooth germ cells and were subsequently harvested from culture plate as a contiguous cell sheet with abundant extracellular matrix. The detached cell–matrix membrane spontaneously contracted to produce a single-cell pellet. The PDLSCs embedded within this cell–matrix complex exhibited several phenotypic characteristics of cementoblast lineages, as indicated by upregulated alkaline phosphatase activity, accelerated mineralization, and the expression of bone sialoprotein and osteocalcin genes. When this PDLSC pellets were transplanted into immunoc...
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apical tooth germ cell conditioned medium enhances the differentiation of Periodontal Ligament stem cells into cementum Periodontal Ligament like tissues
Journal of Periodontal Research, 2009Co-Authors: Zhenhua Yang, X J Zhang, N N Dang, Z F, Y J Sun, Yinzhong Duan, Z Lin, Yan JinAbstract:Background and Objective: Limitations of current Periodontal regeneration modalities in both predictability and extent of healing response, especially on new cementum and attachment formation, underscore the importance of restoring or providing a microenvironment that is capable of promoting the differentiatiation of Periodontal Ligament stem cells (PDLSCs) towards cementoblast-like cells and the formation of cementum/Periodontal Ligament-like tissues. The aim of this study was to investigate the biological effect of conditioned medium from developing apical tooth germ cells (APTG-CM) on the differentiation and cementogenesis of PDLSCs both in vitro and in vivo. Material and Methods: Using the limiting dilution technique, single-colony-derived human PDLSCs were isolated and expanded to obtain homogeneous populations of PDLSCs. Morphological appearance, cell cycle analysis, bromodeoxyuridine incorporation, alkaline phosphatase (ALP) activity, mineralization behavior, gene expression of cementoblast phenotype and in vivo differentiation capacities of PDLSCs co-cultured with APTG-CM were evaluated. Results: The induced PDLSCs exhibited several characteristics of cementoblast lineages, as indicated by the morphological changes, increased proliferation, high ALP activity, and the expression of cementum-related genes and calcified nodule formation in vitro. When transplanted into immunocompromised mice, the induced PDLSCs showed tissue-regenerative capacity to produce cementum/Periodontal Ligament-like structures, characterized by a layer of cementum-like mineralized tissues and associated Periodontal Ligament-like collagen fibers connecting with the newly formed cementum-like deposits, whereas control, untreated PDLSCs transplants mainly formed connective tissues. Conclusion: Our findings suggest that APTG-CM is able to provide a cementogenic microenvironment and induce differentiation of PDLSCs along the cementoblastic lineage. This has important implications for Periodontal engineering.
Isao Ishikawa - One of the best experts on this subject based on the ideXlab platform.
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cementum Periodontal Ligament complex regeneration using the cell sheet technique
Journal of Periodontal Research, 2008Co-Authors: Gomez M Flores, Masayuki Yamato, Teruo Okano, Masateru Hasegawa, R Takagi, Isao IshikawaAbstract:Background and Objective: In the present study we evaluated if a multilayered human Periodontal Ligament cell sheet could reconstruct the physiological architecture of a Periodontal Ligament–cementum complex. Material and methods: Human Periodontal Ligament cells were isolated and then cultured in dishes coated with a temperature-responsive polymer to allow cell detachment as a cell sheet. In the control group, human Periodontal Ligament cells were cultured in Dulbecco’s modified Eagle’s minimal essential medium containing 10% fetal bovine serum and 1% antibiotics. In the experimental group, human Periodontal Ligament cells were cultured in Dulbecco’s modified Eagle’s minimal essential medium and osteodifferentiation medium containing dexamethasone, ascorbic acid and β-glycerophosphate. After 3 wk, scanning electron microscopy was carried out, in addition to staining for alkaline phosphatase activity and for calcium (using the Von Kossa stain). Then human Periodontal Ligament cell sheets were multilayered and placed onto dentin blocks. The constructs were transplanted subcutaneously into the back of immunodeficient rats. At 1 and 6 wk after transplantation, the animals were killed. Demineralized tissue sections were stained using hematoxylin and eosin, and Azan, and then analyzed. Results: After 3 wk of culture in osteodifferentiation medium, human Periodontal Ligament cells produced mineral-like nodules and also showed positive staining for alkaline phosphatase, calcium (Von Kossa) and mRNA expression of type I collagen. By contrast, in the control group only weak alkaline phosphatase staining was observed, the Von Kossa stain was negative and there was no mRNA expression of type I collagen. Six weeks after transplantation with human Periodontal Ligament cells cultured in osteodifferentiation medium, most of the dentin surfaces showed a newly immature cementum-like tissue formation and Periodontal Ligament with perpendicular orientation inserted into the newly deposited cementum-like tissue. Conclusion: This study suggests that the multilayered temperature-responsive culture system can be used as a novel strategy for Periodontal regeneration. The human Periodontal Ligament cell sheet technique may be applicable for regeneration of the clinical Periodontal Ligament–cementum complex.
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application of Periodontal Ligament cell sheet for Periodontal regeneration a pilot study in beagle dogs
Journal of Periodontal Research, 2005Co-Authors: Tatsuya Akizuki, Motohiro Komaki, Hiroaki Tsuchioka, Noriko Kawakatsu, Akihiko Kikuchi, Masayuki Yamato, Teruo Okano, Isao IshikawaAbstract:Objective: The ultimate goal of Periodontal treatment is to regenerate the damaged Periodontal support. Although Periodontal Ligament (PDL) cells are essential for Periodontal regeneration, few studies have reported the transplantation of Periodontal Ligament cells to Periodontal defects. We developed a new method to apply Periodontal Ligament cells as a sheet to the defect. The aim of this study was to investigate the Periodontal healing after application of the Periodontal Ligament cell sheet in beagle dogs. Methods: Autologous Periodontal Ligament cells were obtained from extracted premolars of each beagle dog. Periodontal Ligament cell sheets were fabricated using a temperature-responsive cell culture dish. Dehiscence defects were surgically created on the buccal surface of the mesial roots of bilateral mandibular first molars of each dog. In the experimental group (five defects), Periodontal Ligament cell sheet with reinforced hyaluronic acid carrier was applied to the defect. Only the hyaluronic acid carrier was applied to the contralateral side as a control (five defects). Eight weeks after surgery, the animals were sacrificed and decalcified specimens were prepared. Healing of the Periodontal defects was evaluated histologically and histometrically. Results: No clinical signs of inflammation or recession of gingiva were observed in both experimental and control groups. In the experimental group, Periodontal tissue healing with bone, Periodontal Ligament and cementum formation was observed in three out of five defects. In the control group, such Periodontal tissue formation was not observed except in one defect. Histometric analysis revealed that the formation of new cementum in the experimental group was significantly higher than that in the control group. Conclusion: The Periodontal Ligament cell sheet has a potential to regenerate Periodontal tissue and may become a novel regenerative therapy.
Cedryck Vaquette - One of the best experts on this subject based on the ideXlab platform.
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a biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone Periodontal Ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Wei Fan, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the design of a biphasic scaffold composed of a Fused Deposition Modeling scaffold (bone compartment) and an electrospun membrane (Periodontal compartment) for Periodontal regeneration. In order to achieve simultaneous alveolar bone and Periodontal Ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple Periodontal Ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, Periodontal Ligament and cementum. © 2012 Elsevier Ltd.
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a biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone Periodontal Ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:Abstract This study describes the design of a biphasic scaffold composed of a Fused Deposition Modeling scaffold (bone compartment) and an electrospun membrane (Periodontal compartment) for Periodontal regeneration. In order to achieve simultaneous alveolar bone and Periodontal Ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple Periodontal Ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, Periodontal Ligament and cementum.
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a biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone Periodontal Ligament complex
Institute of Health and Biomedical Innovation; Science & Engineering Faculty, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the design of a biphasic scaffold composed of a Fused Deposition Modeling scaffold (bone compartment) and an electrospun membrane (Periodontal compartment) for Periodontal regeneration. In order to achieve simultaneous alveolar bone and Periodontal Ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple Periodontal Ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, Periodontal Ligament and cementum. © 2012 Elsevier Ltd.