The Experts below are selected from a list of 408 Experts worldwide ranked by ideXlab platform

Xuehong Zhang - One of the best experts on this subject based on the ideXlab platform.

  • identification of a novel bioactive Phenazine Derivative and regulation of phop on its production in streptomyces lomondensis s015
    Journal of Agricultural and Food Chemistry, 2021
    Co-Authors: Ruxiang Deng, Wei Wang, Zhuo Zhang, Xuehong Zhang
    Abstract:

    Natural Phenazines are a class of multifunctional secondary metabolites of bacteria that play an important role in the biocontrol of plant pathogens. In this paper, a novel bioactive Phenazine Derivative was isolated from Streptomyces lomondensis S015 through silica gel chromatography and preparative high-performance liquid chromatography (HPLC). The structure was identified as 1-carboxyl-6-formyl-4,7,9-trihydroxy-Phenazine (CFTHP) by NMR spectroscopy in combination with ultraperformance liquid chromatography & mass spectrometry (UPLC-MS). CFTHP could inhibit Pythium ultimum, Rhizoctonia solani, Septoria steviae, and Fusarium oxysporum f. sp. niveum with minimal inhibitory concentration (MIC) values of 16, 32, 16, and 16 μg/mL, respectively. A global regulatory gene phoP could positively regulate CFTHP biosynthesis since its production was 3.0-fold enhanced by phoP overexpression and inhibited by phoP deletion in Streptomyces lomondensis S015. These studies illustrated the potential of CFTHP as a promising biopesticide and provided a reference for Phenazine production improvement.

  • designing an artificial pathway for the biosynthesis of a novel Phenazine n oxide in pseudomonas chlororaphis ht66
    ACS Synthetic Biology, 2020
    Co-Authors: Shuqi Guo, Wei Wang, Rongfeng Liu, Xuehong Zhang
    Abstract:

    Aromatic N-oxides are valuable due to their versatile chemical, pharmaceutical, and agricultural applications. Natural Phenazine N-oxides possess potent biological activities and can be applied in many ways; however, few N-oxides have been identified. Herein, we developed a microbial system to synthesize Phenazine N-oxides via an artificial pathway. First, the N-monooxygenase NaphzNO1 was predicted and screened in Nocardiopsis sp. 13-12-13 through a product comparison and gene sequencing. Subsequently, according to similarities in the chemical structures of substrates, an artificial pathway for the synthesis of a Phenazine N-oxide in Pseudomonas chlororaphis HT66 was designed and established using three heterologous enzymes, a monooxygenase (PhzS) from P. aeruginosa PAO1, a monooxygenase (PhzO) from P. chlororaphis GP72, and the N-monooxygenase NaphzNO1. A novel Phenazine Derivative, 1-hydroxyPhenazine N'10-oxide, was obtained in an engineered strain, P. chlororaphis HT66-SN. The Phenazine N-monooxygenase NaphzNO1 was identified by metabolically engineering the Phenazine-producing platform P. chlororaphis HT66. Moreover, the function of NaphzNO1, which can catalyze the conversion of 1-hydroxyPhenazine but not that of 2-hydroxyPhenazine, was confirmed in vitro. Additionally, 1-hydroxyPhenazine N'10-oxide demonstrated substantial cytotoxic activity against two human cancer cell lines, MCF-7 and HT-29. Furthermore, the highest microbial production of 1-hydroxyPhenazine N'10-oxide to date was achieved at 143.4 mg/L in the metabolically engineered strain P3-SN. These findings demonstrate that P. chlororaphis HT66 has the potential to be engineered as a platform for Phenazine-modifying gene identification and Derivative production. The present study also provides a promising alternative for the sustainable synthesis of aromatic N-oxides with unique chemical structures by N-monooxygenase.

  • Enhanced biosynthesis of Phenazine-1-carboxamide by engineered Pseudomonas chlororaphis HT66.
    Microbial cell factories, 2018
    Co-Authors: Huasong Peng, Muhammad Bilal, Wei Wang, Zhang Pingyuan, Xuehong Zhang
    Abstract:

    Phenazine-1-carboxamide (PCN), a Phenazine Derivative, is strongly antagonistic to fungal phytopathogens. The high PCN biocontrol activity fascinated researcher’s attention in isolating and identifying novel bacterial strains combined with engineering strategies to target PCN as a lead molecule. The chemical route for Phenazines biosynthesis employs toxic chemicals and display low productivities, require harsh reaction conditions, and generate toxic by-products. Phenazine biosynthesis using some natural Phenazine-producers represent remarkable advantages of non-toxicity and possibly high yield in environmentally-friendlier settings. A biocontrol bacterium with antagonistic activity towards fungal plant pathogens, designated as strain HT66, was isolated from the rice rhizosphere. The strain HT66 was identified as Pseudomonas chlororaphis based on the colony morphology, gas chromatography of cellular fatty acids and 16S rDNA sequence analysis. The secondary metabolite produced by HT66 strain was purified and identified as PCN through mass spectrometry, and 1H, 13C nuclear magnetic resonance spectrum. The yield of PCN by wild-type strain HT66 was 424.87 mg/L at 24 h. The inactivation of psrA and rpeA increased PCN production by 1.66- and 3.06-fold, respectively, which suggests that psrA and rpeA are PCN biosynthesis repressors. qRT-PCR analysis showed that the expression of phzI, phzR, and phzE was markedly increased in the psrA and rpeA double mutant than in psrA or rpeA mutant. However, the transcription level of rpeA and rpeB in strain HT66ΔpsrA increased by 3.52- and 11.58-folds, respectively. The reduced psrA expression in HT66ΔrpeA strain evidenced a complex regulation mechanism for PCN production in HT66. In conclusion, the results evidence that P. chlororaphis HT66 could be modified as a potential cell factory for industrial-scale biosynthesis of PCN and other Phenazine Derivatives by metabolic engineering strategies.

  • Engineering and systems-level analysis of Pseudomonas chlororaphis for production of Phenazine-1-carboxamide using glycerol as the cost-effective carbon source
    BMC, 2018
    Co-Authors: Ruilian Yao, Huasong Peng, Keli Pan, Lei Feng, Xuehong Zhang
    Abstract:

    Abstract Background Glycerol, an inevitable byproduct of biodiesel, has become an attractive feedstock for the production of value-added chemicals due to its availability and low price. Pseudomonas chlororaphis HT66 can use glycerol to synthesize Phenazine-1-carboxamide (PCN), a Phenazine Derivative, which is strongly antagonistic to fungal phytopathogens. A systematic understanding of underlying mechanisms for the PCN overproduction will be important for the further improvement and industrialization. Results We constructed a PCN-overproducing strain (HT66LSP) through knocking out three negative regulatory genes, lon, parS, and prsA in HT66. The strain HT66LSP produced 4.10 g/L of PCN with a yield of 0.23 (g/g) from glycerol, which was of the highest titer and the yield obtained among PCN-producing strains. We studied gene expression, metabolomics, and dynamic 13C tracer in HT66 and HT66LSP. In response to the phenotype changes, the transcript levels of phz biosynthetic genes, which are responsible for PCN biosynthesis, were all upregulated in HT66LSP. Central carbon was rerouted to the shikimate pathway, which was shown by the modulation of specific genes involved in the lower glycolysis, the TCA cycle, and the shikimate pathway, as well as changes in abundances of intracellular metabolites and flux distribution to increase the precursor availability for PCN biosynthesis. Moreover, dynamic 13C-labeling experiments revealed that the presence of metabolite channeling of 3-phosphoglyceric acid to phosphoenolpyruvate and shikimate to trans-2,3-dihydro-3-hydroxyanthranilic acid in HT66LSP could enable high-yielding synthesis of PCN. Conclusions The integrated analysis of gene expression, metabolomics, and dynamic 13C tracer enabled us to gain a more in-depth insight into complex mechanisms for the PCN overproduction. This study provides important basis for further engineering P. chlororaphis for high PCN production and efficient glycerol conversion

  • Enhanced biosynthesis of Phenazine-1-carboxamide by engineered Pseudomonas chlororaphis HT66
    'Springer Science and Business Media LLC', 2018
    Co-Authors: Huasong Peng, Muhammad Bilal, Wei Wang, Pingyuan Zhang, Xuehong Zhang
    Abstract:

    Abstract Background Phenazine-1-carboxamide (PCN), a Phenazine Derivative, is strongly antagonistic to fungal phytopathogens. The high PCN biocontrol activity fascinated researcher’s attention in isolating and identifying novel bacterial strains combined with engineering strategies to target PCN as a lead molecule. The chemical route for Phenazines biosynthesis employs toxic chemicals and display low productivities, require harsh reaction conditions, and generate toxic by-products. Phenazine biosynthesis using some natural Phenazine-producers represent remarkable advantages of non-toxicity and possibly high yield in environmentally-friendlier settings. Results A biocontrol bacterium with antagonistic activity towards fungal plant pathogens, designated as strain HT66, was isolated from the rice rhizosphere. The strain HT66 was identified as Pseudomonas chlororaphis based on the colony morphology, gas chromatography of cellular fatty acids and 16S rDNA sequence analysis. The secondary metabolite produced by HT66 strain was purified and identified as PCN through mass spectrometry, and 1H, 13C nuclear magnetic resonance spectrum. The yield of PCN by wild-type strain HT66 was 424.87 mg/L at 24 h. The inactivation of psrA and rpeA increased PCN production by 1.66- and 3.06-fold, respectively, which suggests that psrA and rpeA are PCN biosynthesis repressors. qRT-PCR analysis showed that the expression of phzI, phzR, and phzE was markedly increased in the psrA and rpeA double mutant than in psrA or rpeA mutant. However, the transcription level of rpeA and rpeB in strain HT66ΔpsrA increased by 3.52- and 11.58-folds, respectively. The reduced psrA expression in HT66ΔrpeA strain evidenced a complex regulation mechanism for PCN production in HT66. Conclusion In conclusion, the results evidence that P. chlororaphis HT66 could be modified as a potential cell factory for industrial-scale biosynthesis of PCN and other Phenazine Derivatives by metabolic engineering strategies

Youming Zhang - One of the best experts on this subject based on the ideXlab platform.

  • fabrication of a solid sensor based on a Phenazine Derivative film for enhancing the sensing properties of biogenic amine and applying for monitoring shrimp freshness
    New Journal of Chemistry, 2021
    Co-Authors: Youming Zhang, Qi Lin, Hong Yao, Taibao Wei
    Abstract:

    In practical applications, fixing a fluorescent sensor on a solid composite film matrix can settle the boundaries of keeping the sensor in the solution state. Herein, we first presented a preparation of anime-response membrane by utilizing the edible gelatin as a film-forming skeleton to immobilize the dual Phenazine Derivative (PD-6), acting as amine-responsive functional units to fabricate a novel solid sensor. In order to ensure the sensor (PD-6 film) is available in the actual application, the mechanical properties, anime-responsive performance, and further application for monitoring shrimp freshness were fully characterized. Those observations indicated that the PD-6 film not only enhanced the sensitivity to anime, but also strengthened the application potential as a solid fluorescent sensor with flexible recyclability and favorable antibacterial properties. It is hoped that this valuable strategy could open a new avenue to overcome the application limitations of the sensor in the solution state.

  • design and fabricating biogenic amine responsive platform based on self assembly property of Phenazine Derivative for visual monitoring of meat spoilage
    Sensors and Actuators B-chemical, 2021
    Co-Authors: Yuxin Che, Youming Zhang, Qi Lin, Hong Yao, Taibao Wei
    Abstract:

    Abstract Smart label serving as a response to a particular stimulus (e.g., biogenic amines) endow great potential to monitor the storage conditions and food freshness. Therefore, the development of smart food packaging for supervising the degree of food decay in real-time is of great importance in food safety. To this endeavor, herein we successfully fabricated a novel biogenic amine-responsive platform (P-N) based on supramolecular host-guest interaction, exploiting self-assembly properties of Phenazine Derivatives (PY) and naphthalene diamide (NDI) induced by hydrogen bond. Taking advantage of this feature, we investigated the prominent performance of this biogenic amine-responsive platform (P-N), involving in feasible fabrication pathway, specific response to biogenic amine, persistent stability and further application as a smart label for motoring meat spoilage. Those observations demonstrated that the P-N could self-assembly into well-organized rodlike structure showing strong AIE effect, not only exhibiting high sensitivity and great potential in the practical application, but also yielding an excellent proof-of-concept smart label in supervising the meat spoilage. Such a novel fabrication strategy is expected to provide some guidance to develop various smart food packaging label for motoring food freshness.

  • tailoring an hso4 anion hybrid receptor based on a Phenazine Derivative
    Photochemical and Photobiological Sciences, 2020
    Co-Authors: Hongqiang Dong, Youming Zhang, Qi Lin, Hong Yao, Hailong Yang, Taibao Wei
    Abstract:

    A catechol-functionalized Phenazine imidazole (PD) was tailored with 2,3-diaminoPhenazine and 3,4-dihydroxy benzaldehyde, and it served as a hybrid acceptor for capturing HSO4− anions. The selectivity and sensitivity of the PD receptor for anion sensing were studied. It was found that the PD receptor could not only display a preferable sensitivity to HSO4− ions with a “turn–off” fluorescence response, but also have a strong anti-interference ability toward other common anions, especially basic anions such as CH3COO−, HPO42−, and H2PO4−. The anion recognition mechanism of PD towards HSO4− is based on multiple hydrogen bond interactions. Finally, the strips for anion detection were prepared, which were verified to be a convenient and high-efficiency test kit for detecting HSO4− ions with the naked eye.

  • a self assembled supramolecular gel constructed by Phenazine Derivative and its application in ultrasensitive detection of cyanide
    Dyes and Pigments, 2020
    Co-Authors: Hu Fang, Youming Zhang, Qi Lin, Hong Yao, Taibao Wei, Haohang Yang
    Abstract:

    Abstract In this work, we constructed a supramolecular gel (PN-G) by Phenazine Derivative, which can ultrasensitive detect cyanide (detection limit equals to 4.18 × 10−10 M). The decrease of fluorescent intensity displayed a linear relationship in the range of 0–0.4 equivalents of cyanide. And no significant fluorescence quenching was observed for all used interfering ions. The cyanide recognition mechanism of PN-G was verified by XRD, NMR, MS and SEM. With addition of cyanide to the supramolecular gel (PN-G), cyanide broken π-π stacking of PN-G, and then PN-G undergoes a nucleophilic addition reaction with CN−, resulting in fluorescence quenched.

  • a novel water soluble pillar 5 arene and Phenazine Derivative self assembled pseudorotaxane sensor for the selective detection of hg2 and ag with high selectivity and sensitivity
    New Journal of Chemistry, 2018
    Co-Authors: Youming Zhang, Xiaopeng Chen, Guoyan Liang, Kaipeng Zhong, Qi Lin, Hong Yao, Taibao Wei
    Abstract:

    We design and synthesize a novel bifunctional supramolecular pseudorotaxane chemosensor (G-WAP), which uses water soluble cationic pillar[5]arene (WAP) as the host and a Phenazine imidazole (G) as guest. The real-time fluorescence response did show efficient quenching of the supramolecular pseudorotaxane chemosensor (G-WAP) upon the addition of Ag+ and an obvious fluorescence color change of the supramolecular pseudorotaxane chemosensor (G-WAP) is observed upon the addition of Hg2+. Excellent sensitivity and selectivity toward the Hg2+ and Ag+ detection were verified in the presence of other competitive cations, and the detection limits of G-WAP for Ag+ is 1.2 × 10−8 M, for Hg2+ is 5.0 × 10−7 M. Consequently, this supramolecular pseudorotaxane chemosensor proved to be an efficient tool in the identification of mercury and silver ions in water.

Taibao Wei - One of the best experts on this subject based on the ideXlab platform.

  • fabrication of a solid sensor based on a Phenazine Derivative film for enhancing the sensing properties of biogenic amine and applying for monitoring shrimp freshness
    New Journal of Chemistry, 2021
    Co-Authors: Youming Zhang, Qi Lin, Hong Yao, Taibao Wei
    Abstract:

    In practical applications, fixing a fluorescent sensor on a solid composite film matrix can settle the boundaries of keeping the sensor in the solution state. Herein, we first presented a preparation of anime-response membrane by utilizing the edible gelatin as a film-forming skeleton to immobilize the dual Phenazine Derivative (PD-6), acting as amine-responsive functional units to fabricate a novel solid sensor. In order to ensure the sensor (PD-6 film) is available in the actual application, the mechanical properties, anime-responsive performance, and further application for monitoring shrimp freshness were fully characterized. Those observations indicated that the PD-6 film not only enhanced the sensitivity to anime, but also strengthened the application potential as a solid fluorescent sensor with flexible recyclability and favorable antibacterial properties. It is hoped that this valuable strategy could open a new avenue to overcome the application limitations of the sensor in the solution state.

  • design and fabricating biogenic amine responsive platform based on self assembly property of Phenazine Derivative for visual monitoring of meat spoilage
    Sensors and Actuators B-chemical, 2021
    Co-Authors: Yuxin Che, Youming Zhang, Qi Lin, Hong Yao, Taibao Wei
    Abstract:

    Abstract Smart label serving as a response to a particular stimulus (e.g., biogenic amines) endow great potential to monitor the storage conditions and food freshness. Therefore, the development of smart food packaging for supervising the degree of food decay in real-time is of great importance in food safety. To this endeavor, herein we successfully fabricated a novel biogenic amine-responsive platform (P-N) based on supramolecular host-guest interaction, exploiting self-assembly properties of Phenazine Derivatives (PY) and naphthalene diamide (NDI) induced by hydrogen bond. Taking advantage of this feature, we investigated the prominent performance of this biogenic amine-responsive platform (P-N), involving in feasible fabrication pathway, specific response to biogenic amine, persistent stability and further application as a smart label for motoring meat spoilage. Those observations demonstrated that the P-N could self-assembly into well-organized rodlike structure showing strong AIE effect, not only exhibiting high sensitivity and great potential in the practical application, but also yielding an excellent proof-of-concept smart label in supervising the meat spoilage. Such a novel fabrication strategy is expected to provide some guidance to develop various smart food packaging label for motoring food freshness.

  • tailoring an hso4 anion hybrid receptor based on a Phenazine Derivative
    Photochemical and Photobiological Sciences, 2020
    Co-Authors: Hongqiang Dong, Youming Zhang, Qi Lin, Hong Yao, Hailong Yang, Taibao Wei
    Abstract:

    A catechol-functionalized Phenazine imidazole (PD) was tailored with 2,3-diaminoPhenazine and 3,4-dihydroxy benzaldehyde, and it served as a hybrid acceptor for capturing HSO4− anions. The selectivity and sensitivity of the PD receptor for anion sensing were studied. It was found that the PD receptor could not only display a preferable sensitivity to HSO4− ions with a “turn–off” fluorescence response, but also have a strong anti-interference ability toward other common anions, especially basic anions such as CH3COO−, HPO42−, and H2PO4−. The anion recognition mechanism of PD towards HSO4− is based on multiple hydrogen bond interactions. Finally, the strips for anion detection were prepared, which were verified to be a convenient and high-efficiency test kit for detecting HSO4− ions with the naked eye.

  • a self assembled supramolecular gel constructed by Phenazine Derivative and its application in ultrasensitive detection of cyanide
    Dyes and Pigments, 2020
    Co-Authors: Hu Fang, Youming Zhang, Qi Lin, Hong Yao, Taibao Wei, Haohang Yang
    Abstract:

    Abstract In this work, we constructed a supramolecular gel (PN-G) by Phenazine Derivative, which can ultrasensitive detect cyanide (detection limit equals to 4.18 × 10−10 M). The decrease of fluorescent intensity displayed a linear relationship in the range of 0–0.4 equivalents of cyanide. And no significant fluorescence quenching was observed for all used interfering ions. The cyanide recognition mechanism of PN-G was verified by XRD, NMR, MS and SEM. With addition of cyanide to the supramolecular gel (PN-G), cyanide broken π-π stacking of PN-G, and then PN-G undergoes a nucleophilic addition reaction with CN−, resulting in fluorescence quenched.

  • a novel water soluble pillar 5 arene and Phenazine Derivative self assembled pseudorotaxane sensor for the selective detection of hg2 and ag with high selectivity and sensitivity
    New Journal of Chemistry, 2018
    Co-Authors: Youming Zhang, Xiaopeng Chen, Guoyan Liang, Kaipeng Zhong, Qi Lin, Hong Yao, Taibao Wei
    Abstract:

    We design and synthesize a novel bifunctional supramolecular pseudorotaxane chemosensor (G-WAP), which uses water soluble cationic pillar[5]arene (WAP) as the host and a Phenazine imidazole (G) as guest. The real-time fluorescence response did show efficient quenching of the supramolecular pseudorotaxane chemosensor (G-WAP) upon the addition of Ag+ and an obvious fluorescence color change of the supramolecular pseudorotaxane chemosensor (G-WAP) is observed upon the addition of Hg2+. Excellent sensitivity and selectivity toward the Hg2+ and Ag+ detection were verified in the presence of other competitive cations, and the detection limits of G-WAP for Ag+ is 1.2 × 10−8 M, for Hg2+ is 5.0 × 10−7 M. Consequently, this supramolecular pseudorotaxane chemosensor proved to be an efficient tool in the identification of mercury and silver ions in water.

Hong Yao - One of the best experts on this subject based on the ideXlab platform.

  • fabrication of a solid sensor based on a Phenazine Derivative film for enhancing the sensing properties of biogenic amine and applying for monitoring shrimp freshness
    New Journal of Chemistry, 2021
    Co-Authors: Youming Zhang, Qi Lin, Hong Yao, Taibao Wei
    Abstract:

    In practical applications, fixing a fluorescent sensor on a solid composite film matrix can settle the boundaries of keeping the sensor in the solution state. Herein, we first presented a preparation of anime-response membrane by utilizing the edible gelatin as a film-forming skeleton to immobilize the dual Phenazine Derivative (PD-6), acting as amine-responsive functional units to fabricate a novel solid sensor. In order to ensure the sensor (PD-6 film) is available in the actual application, the mechanical properties, anime-responsive performance, and further application for monitoring shrimp freshness were fully characterized. Those observations indicated that the PD-6 film not only enhanced the sensitivity to anime, but also strengthened the application potential as a solid fluorescent sensor with flexible recyclability and favorable antibacterial properties. It is hoped that this valuable strategy could open a new avenue to overcome the application limitations of the sensor in the solution state.

  • design and fabricating biogenic amine responsive platform based on self assembly property of Phenazine Derivative for visual monitoring of meat spoilage
    Sensors and Actuators B-chemical, 2021
    Co-Authors: Yuxin Che, Youming Zhang, Qi Lin, Hong Yao, Taibao Wei
    Abstract:

    Abstract Smart label serving as a response to a particular stimulus (e.g., biogenic amines) endow great potential to monitor the storage conditions and food freshness. Therefore, the development of smart food packaging for supervising the degree of food decay in real-time is of great importance in food safety. To this endeavor, herein we successfully fabricated a novel biogenic amine-responsive platform (P-N) based on supramolecular host-guest interaction, exploiting self-assembly properties of Phenazine Derivatives (PY) and naphthalene diamide (NDI) induced by hydrogen bond. Taking advantage of this feature, we investigated the prominent performance of this biogenic amine-responsive platform (P-N), involving in feasible fabrication pathway, specific response to biogenic amine, persistent stability and further application as a smart label for motoring meat spoilage. Those observations demonstrated that the P-N could self-assembly into well-organized rodlike structure showing strong AIE effect, not only exhibiting high sensitivity and great potential in the practical application, but also yielding an excellent proof-of-concept smart label in supervising the meat spoilage. Such a novel fabrication strategy is expected to provide some guidance to develop various smart food packaging label for motoring food freshness.

  • tailoring an hso4 anion hybrid receptor based on a Phenazine Derivative
    Photochemical and Photobiological Sciences, 2020
    Co-Authors: Hongqiang Dong, Youming Zhang, Qi Lin, Hong Yao, Hailong Yang, Taibao Wei
    Abstract:

    A catechol-functionalized Phenazine imidazole (PD) was tailored with 2,3-diaminoPhenazine and 3,4-dihydroxy benzaldehyde, and it served as a hybrid acceptor for capturing HSO4− anions. The selectivity and sensitivity of the PD receptor for anion sensing were studied. It was found that the PD receptor could not only display a preferable sensitivity to HSO4− ions with a “turn–off” fluorescence response, but also have a strong anti-interference ability toward other common anions, especially basic anions such as CH3COO−, HPO42−, and H2PO4−. The anion recognition mechanism of PD towards HSO4− is based on multiple hydrogen bond interactions. Finally, the strips for anion detection were prepared, which were verified to be a convenient and high-efficiency test kit for detecting HSO4− ions with the naked eye.

  • a self assembled supramolecular gel constructed by Phenazine Derivative and its application in ultrasensitive detection of cyanide
    Dyes and Pigments, 2020
    Co-Authors: Hu Fang, Youming Zhang, Qi Lin, Hong Yao, Taibao Wei, Haohang Yang
    Abstract:

    Abstract In this work, we constructed a supramolecular gel (PN-G) by Phenazine Derivative, which can ultrasensitive detect cyanide (detection limit equals to 4.18 × 10−10 M). The decrease of fluorescent intensity displayed a linear relationship in the range of 0–0.4 equivalents of cyanide. And no significant fluorescence quenching was observed for all used interfering ions. The cyanide recognition mechanism of PN-G was verified by XRD, NMR, MS and SEM. With addition of cyanide to the supramolecular gel (PN-G), cyanide broken π-π stacking of PN-G, and then PN-G undergoes a nucleophilic addition reaction with CN−, resulting in fluorescence quenched.

  • a novel water soluble pillar 5 arene and Phenazine Derivative self assembled pseudorotaxane sensor for the selective detection of hg2 and ag with high selectivity and sensitivity
    New Journal of Chemistry, 2018
    Co-Authors: Youming Zhang, Xiaopeng Chen, Guoyan Liang, Kaipeng Zhong, Qi Lin, Hong Yao, Taibao Wei
    Abstract:

    We design and synthesize a novel bifunctional supramolecular pseudorotaxane chemosensor (G-WAP), which uses water soluble cationic pillar[5]arene (WAP) as the host and a Phenazine imidazole (G) as guest. The real-time fluorescence response did show efficient quenching of the supramolecular pseudorotaxane chemosensor (G-WAP) upon the addition of Ag+ and an obvious fluorescence color change of the supramolecular pseudorotaxane chemosensor (G-WAP) is observed upon the addition of Hg2+. Excellent sensitivity and selectivity toward the Hg2+ and Ag+ detection were verified in the presence of other competitive cations, and the detection limits of G-WAP for Ag+ is 1.2 × 10−8 M, for Hg2+ is 5.0 × 10−7 M. Consequently, this supramolecular pseudorotaxane chemosensor proved to be an efficient tool in the identification of mercury and silver ions in water.

Qi Lin - One of the best experts on this subject based on the ideXlab platform.

  • fabrication of a solid sensor based on a Phenazine Derivative film for enhancing the sensing properties of biogenic amine and applying for monitoring shrimp freshness
    New Journal of Chemistry, 2021
    Co-Authors: Youming Zhang, Qi Lin, Hong Yao, Taibao Wei
    Abstract:

    In practical applications, fixing a fluorescent sensor on a solid composite film matrix can settle the boundaries of keeping the sensor in the solution state. Herein, we first presented a preparation of anime-response membrane by utilizing the edible gelatin as a film-forming skeleton to immobilize the dual Phenazine Derivative (PD-6), acting as amine-responsive functional units to fabricate a novel solid sensor. In order to ensure the sensor (PD-6 film) is available in the actual application, the mechanical properties, anime-responsive performance, and further application for monitoring shrimp freshness were fully characterized. Those observations indicated that the PD-6 film not only enhanced the sensitivity to anime, but also strengthened the application potential as a solid fluorescent sensor with flexible recyclability and favorable antibacterial properties. It is hoped that this valuable strategy could open a new avenue to overcome the application limitations of the sensor in the solution state.

  • design and fabricating biogenic amine responsive platform based on self assembly property of Phenazine Derivative for visual monitoring of meat spoilage
    Sensors and Actuators B-chemical, 2021
    Co-Authors: Yuxin Che, Youming Zhang, Qi Lin, Hong Yao, Taibao Wei
    Abstract:

    Abstract Smart label serving as a response to a particular stimulus (e.g., biogenic amines) endow great potential to monitor the storage conditions and food freshness. Therefore, the development of smart food packaging for supervising the degree of food decay in real-time is of great importance in food safety. To this endeavor, herein we successfully fabricated a novel biogenic amine-responsive platform (P-N) based on supramolecular host-guest interaction, exploiting self-assembly properties of Phenazine Derivatives (PY) and naphthalene diamide (NDI) induced by hydrogen bond. Taking advantage of this feature, we investigated the prominent performance of this biogenic amine-responsive platform (P-N), involving in feasible fabrication pathway, specific response to biogenic amine, persistent stability and further application as a smart label for motoring meat spoilage. Those observations demonstrated that the P-N could self-assembly into well-organized rodlike structure showing strong AIE effect, not only exhibiting high sensitivity and great potential in the practical application, but also yielding an excellent proof-of-concept smart label in supervising the meat spoilage. Such a novel fabrication strategy is expected to provide some guidance to develop various smart food packaging label for motoring food freshness.

  • tailoring an hso4 anion hybrid receptor based on a Phenazine Derivative
    Photochemical and Photobiological Sciences, 2020
    Co-Authors: Hongqiang Dong, Youming Zhang, Qi Lin, Hong Yao, Hailong Yang, Taibao Wei
    Abstract:

    A catechol-functionalized Phenazine imidazole (PD) was tailored with 2,3-diaminoPhenazine and 3,4-dihydroxy benzaldehyde, and it served as a hybrid acceptor for capturing HSO4− anions. The selectivity and sensitivity of the PD receptor for anion sensing were studied. It was found that the PD receptor could not only display a preferable sensitivity to HSO4− ions with a “turn–off” fluorescence response, but also have a strong anti-interference ability toward other common anions, especially basic anions such as CH3COO−, HPO42−, and H2PO4−. The anion recognition mechanism of PD towards HSO4− is based on multiple hydrogen bond interactions. Finally, the strips for anion detection were prepared, which were verified to be a convenient and high-efficiency test kit for detecting HSO4− ions with the naked eye.

  • a self assembled supramolecular gel constructed by Phenazine Derivative and its application in ultrasensitive detection of cyanide
    Dyes and Pigments, 2020
    Co-Authors: Hu Fang, Youming Zhang, Qi Lin, Hong Yao, Taibao Wei, Haohang Yang
    Abstract:

    Abstract In this work, we constructed a supramolecular gel (PN-G) by Phenazine Derivative, which can ultrasensitive detect cyanide (detection limit equals to 4.18 × 10−10 M). The decrease of fluorescent intensity displayed a linear relationship in the range of 0–0.4 equivalents of cyanide. And no significant fluorescence quenching was observed for all used interfering ions. The cyanide recognition mechanism of PN-G was verified by XRD, NMR, MS and SEM. With addition of cyanide to the supramolecular gel (PN-G), cyanide broken π-π stacking of PN-G, and then PN-G undergoes a nucleophilic addition reaction with CN−, resulting in fluorescence quenched.

  • a novel water soluble pillar 5 arene and Phenazine Derivative self assembled pseudorotaxane sensor for the selective detection of hg2 and ag with high selectivity and sensitivity
    New Journal of Chemistry, 2018
    Co-Authors: Youming Zhang, Xiaopeng Chen, Guoyan Liang, Kaipeng Zhong, Qi Lin, Hong Yao, Taibao Wei
    Abstract:

    We design and synthesize a novel bifunctional supramolecular pseudorotaxane chemosensor (G-WAP), which uses water soluble cationic pillar[5]arene (WAP) as the host and a Phenazine imidazole (G) as guest. The real-time fluorescence response did show efficient quenching of the supramolecular pseudorotaxane chemosensor (G-WAP) upon the addition of Ag+ and an obvious fluorescence color change of the supramolecular pseudorotaxane chemosensor (G-WAP) is observed upon the addition of Hg2+. Excellent sensitivity and selectivity toward the Hg2+ and Ag+ detection were verified in the presence of other competitive cations, and the detection limits of G-WAP for Ag+ is 1.2 × 10−8 M, for Hg2+ is 5.0 × 10−7 M. Consequently, this supramolecular pseudorotaxane chemosensor proved to be an efficient tool in the identification of mercury and silver ions in water.