The Experts below are selected from a list of 183 Experts worldwide ranked by ideXlab platform
Sylvie Dinant - One of the best experts on this subject based on the ideXlab platform.
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Looking deep inside the Phloem and xylem Cell wall composition by synchrotron FTIR and Raman spectroscopy
2019Co-Authors: Sylvie Dinant, Federica De Marco, Nelly Wolff, Françoise Vilaine, Lionel Gissot, Catherine Bellini, Christophe Sandt, Emilie Aubry, Rozenn Le HirAbstract:Cell walls are highly complex structures that are modified during plant growth and Development. For example, the development of Phloem and xylem vascular Cells, which participate in the transport of sugars and water as well as providing support, can be influenced by Cell-specific wall composition. We used synchrotron radiation-based Fourier-transform infrared (SR-FTIR) and Raman spectroscopy to analyze the Cell wall composition of floral stem vascular tissues of wild-type Arabidopsis and the double-mutant sweet11-1 sweet12-1, which has impaired sugar transport. The SR-FTIR spectra showed that in addition to modified xylem Cell wall composition, Phloem Cell walls in the double mutant line were characterized by modified hemiCellulose composition. Combining Raman spectroscopy with a classification and regression tree (CART) method identified combinations of Raman shifts that could distinguish xylem vessels and fibers. In addition, the disruption of the SWEET11 and SWEET12 genes impacted on xylem wall composition in a Cell-specific manner, with changes in hemiCelluloses and Cellulose observed at the xylem vessel interface. These results suggest that the facilitated transport of sugars by transporters that exist between vascular parenchyma Cells and conducting Cells is important in ensuring correct Phloem and xylem Cell wall composition.
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Live-Cell imaging of fluorescently tagged Phloem proteins with confocal microscopy
2019Co-Authors: Thibaud Cayla, Rozenn Le Hir, Sylvie DinantAbstract:Confocal laser scanning microscopy can enable observation of Phloem Cells in living tissues. Here we describe live imaging of Phloem Cells in the leaves and roots of Arabidopsis thaliana using fluorescently tagged proteins, either expressed in the vasculature using Phloem specific promoters or constitutively expressed reference marker lines. Now, the majority of Phloem Cell types can be identified, allowing a precise Cellular and subCellular localization of Phloem proteins.
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Synchrotron FTIR and Raman spectroscopy provide unique spectral fingerprints for Arabidopsis floral stem vascular tissues
Journal of Experimental Botany, 2019Co-Authors: Sylvie Dinant, Federica De Marco, Nelly Wolff, Françoise Vilaine, Lionel Gissot, Catherine Bellini, Emilia Aubry, Christophe Sandt, Rozenn Le HirAbstract:Cell walls are highly complex structures that are modified during plant growth and development. For example, the development of Phloem and xylem vascular Cells, which participate in the transport of sugars and water as well as providing support, can be influenced by Cell-specific wall composition. Here, we used synchrotron radiation-based Fourier-transform infrared (SR-FTIR) and Raman spectroscopy to analyse the Cell wall composition of floral stem vascular tissues of wild-type Arabidopsis and the double-mutant sweet11-1 sweet12-1, which has impaired sugar transport. The SR-FTIR spectra showed that in addition to modified xylem Cell wall composition, Phloem Cell walls in the double-mutant line were characterized by modified hemiCellulose composition. Combining Raman spectroscopy with a classification and regression tree (CART) method identified combinations of Raman shifts that could distinguish xylem vessels and fibers. In addition, the disruption of the SWEET11 and SWEET12 genes impacted on xylem wall composition in a Cell-specific manner, with changes in hemiCelluloses and Cellulose observed at the xylem vessel interface. These results suggest that the facilitated transport of sugars by transporters that exist between vascular parenchyma Cells and conducting Cells is important in ensuring correct Phloem and xylem Cell wall composition.
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Synchrotron FTIR and Raman spectroscopy provide unique spectral fingerprints for Arabidopsis floral stem vascular tissues
2018Co-Authors: Sylvie Dinant, Federica De Marco, Nelly Wolff, Françoise Vilaine, Lionel Gissot, Catherine Bellini, Emilia Aubry, Christophe Sandt, Rozenn Le HirAbstract:Cell walls are highly complex structures that are modified during plant growth and development. For example, the development of Phloem and xylem vascular Cells, which participate in the transport of sugars and water as well as support, can be influenced by Cell-specific Cell wall composition. Here, we used synchrotron radiation-based infrared (SR-FTIR) and Raman spectroscopy to analyze the Cell wall composition of wild-type and double mutant sweet11-1sweet12-1, which impairs sugar transport, Arabidopsis floral stem vascular tissue. The FTIR spectra showed that in addition to modified xylem Cell wall composition, Phloem Cell walls in the double mutant line were characterized by modified hemiCellulose composition. Moreover, combining Raman spectroscopy with a Classification and Regression Tree (CART) method identified combinations of Raman shifts that could distinguish xylem vessels and fibers. Additionally, the disruption of SWEET11 and SWEET12 genes impacts xylem Cell wall composition in a Cell-specific manner, with changes in hemiCelluloses and Cellulose observed at the xylem vessel interface. These results suggest that the facilitated transport of sugars by transporters that exist between vascular parenchyma Cells and conducting Cells is important to ensuring correct Phloem and xylem Cell wall composition.
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The vascular tissues in Arabidopsis thaliana floral stem: High spatial resolution using Synchrotron infrared, Raman and deep UV spectroscopy
2017Co-Authors: Rozenn Le Hir, Federica De Marco, Nelly Wolff, Françoise Vilaine, Lionel Gissot, C Sand, Catherine Bellini, Frédéric Jamme, Sylvie DinantAbstract:In higher plants, a network of vascular tissues (the Phloem and the xylem), which supply water, minerals and other nutrients, connects the different organs and provides mechanical support for the aerial organs. The xylem and the Phloem are two highly specialized conductive tissues. The Cell wall surrounding the vascular Cells is reinforced by a complex matrix of sugar-derived compounds (i.e.: Cellulose, hemiCellulose, pectins) and lignin, whose compositions depend on the Cell type and developmental stage. For example low lignin and high hemiCellulose content characterize Phloem Cells, while lignin and xylans are enriched in xylem Cells. The supply and synthesis of the Cell wall precursors take place in specialized parenchyma and fibre Cells, which are responsible of the thickening of the associated conductive Cell walls. As a consequence Phloem and xylem Cells represent a sink for consumption and sequestration of carbohydrates, with a competitive allocation of carbon pools between lignin and Cellulosic compounds, which leads to a negative correlation between biomass production and lignin content. To improve our knowledge on these processes, we focus on the carbohydrate components deposited during Cell wall formation of Phloem and xylem Cells using the Arabidopsis thaliana floral stem as a model for carbon allocation. Our investigations, by label-free Synchrotron FT-IR, Raman and deep ultra violet microspectroscopies, of the Cell wall composition and the cytosolic content of the different xylem and Phloem Cell types will be presented, using the floral stem of both Arabidopsis wild-type plants and mutants affected in sugar homeostasis.
Rozenn Le Hir - One of the best experts on this subject based on the ideXlab platform.
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Looking deep inside the Phloem and xylem Cell wall composition by synchrotron FTIR and Raman spectroscopy
2019Co-Authors: Sylvie Dinant, Federica De Marco, Nelly Wolff, Françoise Vilaine, Lionel Gissot, Catherine Bellini, Christophe Sandt, Emilie Aubry, Rozenn Le HirAbstract:Cell walls are highly complex structures that are modified during plant growth and Development. For example, the development of Phloem and xylem vascular Cells, which participate in the transport of sugars and water as well as providing support, can be influenced by Cell-specific wall composition. We used synchrotron radiation-based Fourier-transform infrared (SR-FTIR) and Raman spectroscopy to analyze the Cell wall composition of floral stem vascular tissues of wild-type Arabidopsis and the double-mutant sweet11-1 sweet12-1, which has impaired sugar transport. The SR-FTIR spectra showed that in addition to modified xylem Cell wall composition, Phloem Cell walls in the double mutant line were characterized by modified hemiCellulose composition. Combining Raman spectroscopy with a classification and regression tree (CART) method identified combinations of Raman shifts that could distinguish xylem vessels and fibers. In addition, the disruption of the SWEET11 and SWEET12 genes impacted on xylem wall composition in a Cell-specific manner, with changes in hemiCelluloses and Cellulose observed at the xylem vessel interface. These results suggest that the facilitated transport of sugars by transporters that exist between vascular parenchyma Cells and conducting Cells is important in ensuring correct Phloem and xylem Cell wall composition.
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Live-Cell imaging of fluorescently tagged Phloem proteins with confocal microscopy
2019Co-Authors: Thibaud Cayla, Rozenn Le Hir, Sylvie DinantAbstract:Confocal laser scanning microscopy can enable observation of Phloem Cells in living tissues. Here we describe live imaging of Phloem Cells in the leaves and roots of Arabidopsis thaliana using fluorescently tagged proteins, either expressed in the vasculature using Phloem specific promoters or constitutively expressed reference marker lines. Now, the majority of Phloem Cell types can be identified, allowing a precise Cellular and subCellular localization of Phloem proteins.
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Synchrotron FTIR and Raman spectroscopy provide unique spectral fingerprints for Arabidopsis floral stem vascular tissues
Journal of Experimental Botany, 2019Co-Authors: Sylvie Dinant, Federica De Marco, Nelly Wolff, Françoise Vilaine, Lionel Gissot, Catherine Bellini, Emilia Aubry, Christophe Sandt, Rozenn Le HirAbstract:Cell walls are highly complex structures that are modified during plant growth and development. For example, the development of Phloem and xylem vascular Cells, which participate in the transport of sugars and water as well as providing support, can be influenced by Cell-specific wall composition. Here, we used synchrotron radiation-based Fourier-transform infrared (SR-FTIR) and Raman spectroscopy to analyse the Cell wall composition of floral stem vascular tissues of wild-type Arabidopsis and the double-mutant sweet11-1 sweet12-1, which has impaired sugar transport. The SR-FTIR spectra showed that in addition to modified xylem Cell wall composition, Phloem Cell walls in the double-mutant line were characterized by modified hemiCellulose composition. Combining Raman spectroscopy with a classification and regression tree (CART) method identified combinations of Raman shifts that could distinguish xylem vessels and fibers. In addition, the disruption of the SWEET11 and SWEET12 genes impacted on xylem wall composition in a Cell-specific manner, with changes in hemiCelluloses and Cellulose observed at the xylem vessel interface. These results suggest that the facilitated transport of sugars by transporters that exist between vascular parenchyma Cells and conducting Cells is important in ensuring correct Phloem and xylem Cell wall composition.
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Synchrotron FTIR and Raman spectroscopy provide unique spectral fingerprints for Arabidopsis floral stem vascular tissues
2018Co-Authors: Sylvie Dinant, Federica De Marco, Nelly Wolff, Françoise Vilaine, Lionel Gissot, Catherine Bellini, Emilia Aubry, Christophe Sandt, Rozenn Le HirAbstract:Cell walls are highly complex structures that are modified during plant growth and development. For example, the development of Phloem and xylem vascular Cells, which participate in the transport of sugars and water as well as support, can be influenced by Cell-specific Cell wall composition. Here, we used synchrotron radiation-based infrared (SR-FTIR) and Raman spectroscopy to analyze the Cell wall composition of wild-type and double mutant sweet11-1sweet12-1, which impairs sugar transport, Arabidopsis floral stem vascular tissue. The FTIR spectra showed that in addition to modified xylem Cell wall composition, Phloem Cell walls in the double mutant line were characterized by modified hemiCellulose composition. Moreover, combining Raman spectroscopy with a Classification and Regression Tree (CART) method identified combinations of Raman shifts that could distinguish xylem vessels and fibers. Additionally, the disruption of SWEET11 and SWEET12 genes impacts xylem Cell wall composition in a Cell-specific manner, with changes in hemiCelluloses and Cellulose observed at the xylem vessel interface. These results suggest that the facilitated transport of sugars by transporters that exist between vascular parenchyma Cells and conducting Cells is important to ensuring correct Phloem and xylem Cell wall composition.
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The vascular tissues in Arabidopsis thaliana floral stem: High spatial resolution using Synchrotron infrared, Raman and deep UV spectroscopy
2017Co-Authors: Rozenn Le Hir, Federica De Marco, Nelly Wolff, Françoise Vilaine, Lionel Gissot, C Sand, Catherine Bellini, Frédéric Jamme, Sylvie DinantAbstract:In higher plants, a network of vascular tissues (the Phloem and the xylem), which supply water, minerals and other nutrients, connects the different organs and provides mechanical support for the aerial organs. The xylem and the Phloem are two highly specialized conductive tissues. The Cell wall surrounding the vascular Cells is reinforced by a complex matrix of sugar-derived compounds (i.e.: Cellulose, hemiCellulose, pectins) and lignin, whose compositions depend on the Cell type and developmental stage. For example low lignin and high hemiCellulose content characterize Phloem Cells, while lignin and xylans are enriched in xylem Cells. The supply and synthesis of the Cell wall precursors take place in specialized parenchyma and fibre Cells, which are responsible of the thickening of the associated conductive Cell walls. As a consequence Phloem and xylem Cells represent a sink for consumption and sequestration of carbohydrates, with a competitive allocation of carbon pools between lignin and Cellulosic compounds, which leads to a negative correlation between biomass production and lignin content. To improve our knowledge on these processes, we focus on the carbohydrate components deposited during Cell wall formation of Phloem and xylem Cells using the Arabidopsis thaliana floral stem as a model for carbon allocation. Our investigations, by label-free Synchrotron FT-IR, Raman and deep ultra violet microspectroscopies, of the Cell wall composition and the cytosolic content of the different xylem and Phloem Cell types will be presented, using the floral stem of both Arabidopsis wild-type plants and mutants affected in sugar homeostasis.
Frederick G. Gmitter - One of the best experts on this subject based on the ideXlab platform.
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Differential anatomical responses of tolerant and susceptible citrus species to the infection of ‘Candidatus Liberibacter asiaticus’
Physiological and Molecular Plant Pathology, 2013Co-Authors: Jing Fan, Diann Achor, Chunxian Chen, Ronald H. Brlansky, Frederick G. GmitterAbstract:Citrus Huanglongbing (HLB) is becoming the most devastating citrus disease worldwide. Although no known HLB-resistant citrus species or varieties have been identified, some citrus accessions such as rough lemon are reportedly tolerant. To better understand the HLB tolerance or susceptibility mechanisms in citrus, comparative anatomical analyses of tolerant rough lemon and sensitive sweet orange seedlings in response to HLB-associated bacterium, ‘Candidatus Liberibacter asiaticus’, were performed on leaf, stem and root tissues using light microscopy and transmission electron microscopy. Phloem collapse, plugged sieve elements and accumulation of starch were observed in leaf petioles of symptomatic leaves from both HLB-diseased rough lemon and sweet orange, while not in the mock-inoculated controls. Interestingly, in symptomless leaves, significant anatomical changes (e.g. Phloem Cell collapse and starch accumulation) were found in HLB-diseased sweet orange, but not in rough lemon. Furthermore, starch depletion, Phloem Cell collapse and absence of Phloem fibers were observed in secondary roots of only diseased sweet orange. In young green stems, a few plugged sieve elements were seen in both diseased rough lemon and sweet orange; whereas starch deposition only occurred in the latter. Taken together at the whole plant level, HLB infection induces fewer disruptive anatomical changes in rough lemon than in sweet orange. In particular, the absence of obvious changes in the rough lemon root system is suggested to be critical for sustaining plant growth after infection, and may contribute greatly to its HLB tolerance.
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comparative transcriptional and anatomical analyses of tolerant rough lemon and susceptible sweet orange in response to candidatus liberibacter asiaticus infection
Molecular Plant-microbe Interactions, 2012Co-Authors: Jing Fan, Diann Achor, Chunxian Chen, Abeer Khalaf, Ronald H. Brlansky, Gloria A. Moore, Frederick G. GmitterAbstract:Although there are no known sources of genetic resistance, some Citrus spp. are reportedly tolerant to huanglongbing (HLB), presumably caused by ‘Candidatus Liberibacter asiaticus’. Time-course transcriptional analysis of tolerant rough lemon (Citrus jambhiri) and susceptible sweet orange (C. sinensis) in response to ‘Ca. L. asiaticus’ infection showed more genes differentially expressed in HLB-affected rough lemon than sweet orange at early stages but substantially fewer at late time points, possibly a critical factor underlying differences in sensitivity to ‘Ca. L. asiaticus’. Pathway analysis revealed that stress responses were distinctively modulated in rough lemon and sweet orange. Although microscopic changes (e.g., callose deposition in sieve elements and Phloem Cell collapse) were found in both infected species, remarkably, Phloem transport activity in midribs of source leaves in rough lemon was much less affected by HLB than in sweet orange. The difference in Phloem Cell transport activities is a...
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Comparative Transcriptional and Anatomical Analyses of Tolerant Rough Lemon and Susceptible Sweet Orange in Response to ‘Candidatus Liberibacter asiaticus’ Infection
Molecular Plant-Microbe Interactions®, 2012Co-Authors: Jing Fan, Diann Achor, Chunxian Chen, Abeer Khalaf, Ronald H. Brlansky, Gloria A. Moore, Frederick G. GmitterAbstract:Although there are no known sources of genetic resistance, some Citrus spp. are reportedly tolerant to huanglongbing (HLB), presumably caused by ‘Candidatus Liberibacter asiaticus’. Time-course transcriptional analysis of tolerant rough lemon (Citrus jambhiri) and susceptible sweet orange (C. sinensis) in response to ‘Ca. L. asiaticus’ infection showed more genes differentially expressed in HLB-affected rough lemon than sweet orange at early stages but substantially fewer at late time points, possibly a critical factor underlying differences in sensitivity to ‘Ca. L. asiaticus’. Pathway analysis revealed that stress responses were distinctively modulated in rough lemon and sweet orange. Although microscopic changes (e.g., callose deposition in sieve elements and Phloem Cell collapse) were found in both infected species, remarkably, Phloem transport activity in midribs of source leaves in rough lemon was much less affected by HLB than in sweet orange. The difference in Phloem Cell transport activities is a...
Jing Fan - One of the best experts on this subject based on the ideXlab platform.
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Differential anatomical responses of tolerant and susceptible citrus species to the infection of ‘Candidatus Liberibacter asiaticus’
Physiological and Molecular Plant Pathology, 2013Co-Authors: Jing Fan, Diann Achor, Chunxian Chen, Ronald H. Brlansky, Frederick G. GmitterAbstract:Citrus Huanglongbing (HLB) is becoming the most devastating citrus disease worldwide. Although no known HLB-resistant citrus species or varieties have been identified, some citrus accessions such as rough lemon are reportedly tolerant. To better understand the HLB tolerance or susceptibility mechanisms in citrus, comparative anatomical analyses of tolerant rough lemon and sensitive sweet orange seedlings in response to HLB-associated bacterium, ‘Candidatus Liberibacter asiaticus’, were performed on leaf, stem and root tissues using light microscopy and transmission electron microscopy. Phloem collapse, plugged sieve elements and accumulation of starch were observed in leaf petioles of symptomatic leaves from both HLB-diseased rough lemon and sweet orange, while not in the mock-inoculated controls. Interestingly, in symptomless leaves, significant anatomical changes (e.g. Phloem Cell collapse and starch accumulation) were found in HLB-diseased sweet orange, but not in rough lemon. Furthermore, starch depletion, Phloem Cell collapse and absence of Phloem fibers were observed in secondary roots of only diseased sweet orange. In young green stems, a few plugged sieve elements were seen in both diseased rough lemon and sweet orange; whereas starch deposition only occurred in the latter. Taken together at the whole plant level, HLB infection induces fewer disruptive anatomical changes in rough lemon than in sweet orange. In particular, the absence of obvious changes in the rough lemon root system is suggested to be critical for sustaining plant growth after infection, and may contribute greatly to its HLB tolerance.
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comparative transcriptional and anatomical analyses of tolerant rough lemon and susceptible sweet orange in response to candidatus liberibacter asiaticus infection
Molecular Plant-microbe Interactions, 2012Co-Authors: Jing Fan, Diann Achor, Chunxian Chen, Abeer Khalaf, Ronald H. Brlansky, Gloria A. Moore, Frederick G. GmitterAbstract:Although there are no known sources of genetic resistance, some Citrus spp. are reportedly tolerant to huanglongbing (HLB), presumably caused by ‘Candidatus Liberibacter asiaticus’. Time-course transcriptional analysis of tolerant rough lemon (Citrus jambhiri) and susceptible sweet orange (C. sinensis) in response to ‘Ca. L. asiaticus’ infection showed more genes differentially expressed in HLB-affected rough lemon than sweet orange at early stages but substantially fewer at late time points, possibly a critical factor underlying differences in sensitivity to ‘Ca. L. asiaticus’. Pathway analysis revealed that stress responses were distinctively modulated in rough lemon and sweet orange. Although microscopic changes (e.g., callose deposition in sieve elements and Phloem Cell collapse) were found in both infected species, remarkably, Phloem transport activity in midribs of source leaves in rough lemon was much less affected by HLB than in sweet orange. The difference in Phloem Cell transport activities is a...
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Comparative Transcriptional and Anatomical Analyses of Tolerant Rough Lemon and Susceptible Sweet Orange in Response to ‘Candidatus Liberibacter asiaticus’ Infection
Molecular Plant-Microbe Interactions®, 2012Co-Authors: Jing Fan, Diann Achor, Chunxian Chen, Abeer Khalaf, Ronald H. Brlansky, Gloria A. Moore, Frederick G. GmitterAbstract:Although there are no known sources of genetic resistance, some Citrus spp. are reportedly tolerant to huanglongbing (HLB), presumably caused by ‘Candidatus Liberibacter asiaticus’. Time-course transcriptional analysis of tolerant rough lemon (Citrus jambhiri) and susceptible sweet orange (C. sinensis) in response to ‘Ca. L. asiaticus’ infection showed more genes differentially expressed in HLB-affected rough lemon than sweet orange at early stages but substantially fewer at late time points, possibly a critical factor underlying differences in sensitivity to ‘Ca. L. asiaticus’. Pathway analysis revealed that stress responses were distinctively modulated in rough lemon and sweet orange. Although microscopic changes (e.g., callose deposition in sieve elements and Phloem Cell collapse) were found in both infected species, remarkably, Phloem transport activity in midribs of source leaves in rough lemon was much less affected by HLB than in sweet orange. The difference in Phloem Cell transport activities is a...
Ronald H. Brlansky - One of the best experts on this subject based on the ideXlab platform.
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Differential anatomical responses of tolerant and susceptible citrus species to the infection of ‘Candidatus Liberibacter asiaticus’
Physiological and Molecular Plant Pathology, 2013Co-Authors: Jing Fan, Diann Achor, Chunxian Chen, Ronald H. Brlansky, Frederick G. GmitterAbstract:Citrus Huanglongbing (HLB) is becoming the most devastating citrus disease worldwide. Although no known HLB-resistant citrus species or varieties have been identified, some citrus accessions such as rough lemon are reportedly tolerant. To better understand the HLB tolerance or susceptibility mechanisms in citrus, comparative anatomical analyses of tolerant rough lemon and sensitive sweet orange seedlings in response to HLB-associated bacterium, ‘Candidatus Liberibacter asiaticus’, were performed on leaf, stem and root tissues using light microscopy and transmission electron microscopy. Phloem collapse, plugged sieve elements and accumulation of starch were observed in leaf petioles of symptomatic leaves from both HLB-diseased rough lemon and sweet orange, while not in the mock-inoculated controls. Interestingly, in symptomless leaves, significant anatomical changes (e.g. Phloem Cell collapse and starch accumulation) were found in HLB-diseased sweet orange, but not in rough lemon. Furthermore, starch depletion, Phloem Cell collapse and absence of Phloem fibers were observed in secondary roots of only diseased sweet orange. In young green stems, a few plugged sieve elements were seen in both diseased rough lemon and sweet orange; whereas starch deposition only occurred in the latter. Taken together at the whole plant level, HLB infection induces fewer disruptive anatomical changes in rough lemon than in sweet orange. In particular, the absence of obvious changes in the rough lemon root system is suggested to be critical for sustaining plant growth after infection, and may contribute greatly to its HLB tolerance.
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comparative transcriptional and anatomical analyses of tolerant rough lemon and susceptible sweet orange in response to candidatus liberibacter asiaticus infection
Molecular Plant-microbe Interactions, 2012Co-Authors: Jing Fan, Diann Achor, Chunxian Chen, Abeer Khalaf, Ronald H. Brlansky, Gloria A. Moore, Frederick G. GmitterAbstract:Although there are no known sources of genetic resistance, some Citrus spp. are reportedly tolerant to huanglongbing (HLB), presumably caused by ‘Candidatus Liberibacter asiaticus’. Time-course transcriptional analysis of tolerant rough lemon (Citrus jambhiri) and susceptible sweet orange (C. sinensis) in response to ‘Ca. L. asiaticus’ infection showed more genes differentially expressed in HLB-affected rough lemon than sweet orange at early stages but substantially fewer at late time points, possibly a critical factor underlying differences in sensitivity to ‘Ca. L. asiaticus’. Pathway analysis revealed that stress responses were distinctively modulated in rough lemon and sweet orange. Although microscopic changes (e.g., callose deposition in sieve elements and Phloem Cell collapse) were found in both infected species, remarkably, Phloem transport activity in midribs of source leaves in rough lemon was much less affected by HLB than in sweet orange. The difference in Phloem Cell transport activities is a...
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Comparative Transcriptional and Anatomical Analyses of Tolerant Rough Lemon and Susceptible Sweet Orange in Response to ‘Candidatus Liberibacter asiaticus’ Infection
Molecular Plant-Microbe Interactions®, 2012Co-Authors: Jing Fan, Diann Achor, Chunxian Chen, Abeer Khalaf, Ronald H. Brlansky, Gloria A. Moore, Frederick G. GmitterAbstract:Although there are no known sources of genetic resistance, some Citrus spp. are reportedly tolerant to huanglongbing (HLB), presumably caused by ‘Candidatus Liberibacter asiaticus’. Time-course transcriptional analysis of tolerant rough lemon (Citrus jambhiri) and susceptible sweet orange (C. sinensis) in response to ‘Ca. L. asiaticus’ infection showed more genes differentially expressed in HLB-affected rough lemon than sweet orange at early stages but substantially fewer at late time points, possibly a critical factor underlying differences in sensitivity to ‘Ca. L. asiaticus’. Pathway analysis revealed that stress responses were distinctively modulated in rough lemon and sweet orange. Although microscopic changes (e.g., callose deposition in sieve elements and Phloem Cell collapse) were found in both infected species, remarkably, Phloem transport activity in midribs of source leaves in rough lemon was much less affected by HLB than in sweet orange. The difference in Phloem Cell transport activities is a...