The Experts below are selected from a list of 3045 Experts worldwide ranked by ideXlab platform
Ravi Naidu - One of the best experts on this subject based on the ideXlab platform.
-
potential application of selected metal resistant Phosphate Solubilizing Bacteria isolated from the gut of earthworm metaphire posthuma in plant growth promotion
Geoderma, 2018Co-Authors: Jayanta Kumar Biswas, Ravi Naidu, Anurupa Banerjee, Mahendra Rai, Bhabananda Biswas, Meththika Vithanage, Madhab Chandra Dash, Santosh Kumar Sarkar, Erik MeersAbstract:Abstract The present study focuses on the isolation of three Phosphate Solubilizing Bacteria (PSB), PSB1, PSB2 and PSB3 from the gut of earthworm Metaphire posthuma. The three stains were identified as Bacillus megaterium (MF 589715), Staphylococcus haemolyticus (MF 589716) and Bacillus licheniformis (MF 589720) through 16 S rRNA gene sequencing and biochemical characterization. The strains showed resistance to the metals Cu and Zn at significant concentrations and could solubilize Phosphate even in the presence of metals. Maximum Phosphate was solubilized by strain PSB3 with a production of 222 ± 2.0 mg L−1 soluble Phosphate followed by PSB1 (213.7 ± 1.3 mg L−1) and PSB2 (193.5 ± 1.5 mg L−1) at 96 h of incubation. The strains were able to produce indole acetic acid (IAA) in presence of l -tryptophan and possessed ammonium ion production potential in the order PSB3 > PSB1 > PSB2 (P PSB1 > PSB2 (P
-
concomitant rock Phosphate dissolution and lead immobilization by Phosphate Solubilizing Bacteria enterobacter sp
Journal of Environmental Management, 2011Co-Authors: Jin-hee Park, Nanthi Bolan, Mallavarapu Megharaj, Ravi NaiduAbstract:Abstract This paper examines the potential value of Phosphate Solubilizing Bacteria (Enterobacter cloacae) in the dissolution of rock Phosphate (RP) and subsequent immobilization of lead (Pb) in both Bacterial growth medium and soils. Enterobacter sp. showed resistance to Pb and the bacterium solubilized 17.5% of RP in the growth medium. Entrobacter sp. did not enhance Pb immobilization in solution because of acidification of Bacterial medium, thereby inhibiting the formation of P-induced Pb precipitation. However, in the case of soil, Enterobacter sp. increased Pb immobilization by 6.98, 25.6 and 32.0% with the RP level of 200, 800 and 1600 mg P/kg, respectively. The immobilization of Pb in Pb-spiked soils was attributed to pyromorphite formation as indicated by XRD analysis. Inoculation of Phosphate Solubilizing Bacteria with RP in soil can be used as an alternative technique to soluble P compounds which can cause eutrophication of surface water.
-
concomitant rock Phosphate dissolution and lead immobilization by Phosphate Solubilizing Bacteria enterobacter sp
Journal of Environmental Management, 2011Co-Authors: Jin-hee Park, Nanthi Bolan, Mallavarapu Megharaj, Ravi NaiduAbstract:This paper examines the potential value of Phosphate Solubilizing Bacteria (Enterobacter cloacae) in the dissolution of rock Phosphate (RP) and subsequent immobilization of lead (Pb) in both Bacterial growth medium and soils. Enterobacter sp. showed resistance to Pb and the bacterium solubilized 17.5% of RP in the growth medium. Enterobacter sp. did not enhance Pb immobilization in solution because of acidification of Bacterial medium, thereby inhibiting the formation of P-induced Pb precipitation. However, in the case of soil, Enterobacter sp. increased Pb immobilization by 6.98, 25.6 and 32.0% with the RP level of 200, 800 and 1600 mg P/kg, respectively. The immobilization of Pb in Pb-spiked soils was attributed to pyromorphite formation as indicated by XRD analysis. Inoculation of Phosphate Solubilizing Bacteria with RP in soil can be used as an alternative technique to soluble P compounds which can cause eutrophication of surface water.
-
isolation of Phosphate Solubilizing Bacteria and their potential for lead immobilization in soil
Journal of Hazardous Materials, 2011Co-Authors: Jin-hee Park, Nanthi Bolan, Mallavarapu Megharaj, Ravi NaiduAbstract:Abstract Lead (Pb), a highly toxic heavy metal forms stable compounds with Phosphate (P). The potential of Phosphate Solubilizing Bacteria (PSB) to immobilize Pb by enhancing solubilization of insoluble P compounds was tested in this research. Eighteen different PSB strains isolated from P amended and Pb contaminated soils were screened for their efficiency in P solubilization. The PSB isolated from P amended soils solubilized 217–479 mg/L of P while the PSB from Pb contaminated soil solubilized 31–293 mg/L of P. Stepwise multiple regression analysis and P solubility kinetics indicated that the major mechanism of P solubilization by PSB is the pH reduction through the release of organic acids. From the isolated Bacteria, two PSB were chosen for Pb immobilization and these Bacteria were identified as Pantoea sp. and Enterobacter sp., respectively. The PSB significantly increased P solubilization by 25.0% and 49.9% in the case of Pantoea sp., and 63.3% and 88.6% in the case of Enterobacter sp. for 200 and 800 mg/kg of rock Phosphate (RP) addition, respectively, thereby enhancing the immobilization of Pb by 8.25–13.7% in the case of Pantoea sp. and 14.7–26.4% in the case of Enterobacter sp. The ability of PSB to solubilize P, promote plant growth, and immobilize Pb can be used for phytostabilization of Pb contaminated soils.
Jin-hee Park - One of the best experts on this subject based on the ideXlab platform.
-
concomitant rock Phosphate dissolution and lead immobilization by Phosphate Solubilizing Bacteria enterobacter sp
Journal of Environmental Management, 2011Co-Authors: Jin-hee Park, Nanthi Bolan, Mallavarapu Megharaj, Ravi NaiduAbstract:Abstract This paper examines the potential value of Phosphate Solubilizing Bacteria (Enterobacter cloacae) in the dissolution of rock Phosphate (RP) and subsequent immobilization of lead (Pb) in both Bacterial growth medium and soils. Enterobacter sp. showed resistance to Pb and the bacterium solubilized 17.5% of RP in the growth medium. Entrobacter sp. did not enhance Pb immobilization in solution because of acidification of Bacterial medium, thereby inhibiting the formation of P-induced Pb precipitation. However, in the case of soil, Enterobacter sp. increased Pb immobilization by 6.98, 25.6 and 32.0% with the RP level of 200, 800 and 1600 mg P/kg, respectively. The immobilization of Pb in Pb-spiked soils was attributed to pyromorphite formation as indicated by XRD analysis. Inoculation of Phosphate Solubilizing Bacteria with RP in soil can be used as an alternative technique to soluble P compounds which can cause eutrophication of surface water.
-
concomitant rock Phosphate dissolution and lead immobilization by Phosphate Solubilizing Bacteria enterobacter sp
Journal of Environmental Management, 2011Co-Authors: Jin-hee Park, Nanthi Bolan, Mallavarapu Megharaj, Ravi NaiduAbstract:This paper examines the potential value of Phosphate Solubilizing Bacteria (Enterobacter cloacae) in the dissolution of rock Phosphate (RP) and subsequent immobilization of lead (Pb) in both Bacterial growth medium and soils. Enterobacter sp. showed resistance to Pb and the bacterium solubilized 17.5% of RP in the growth medium. Enterobacter sp. did not enhance Pb immobilization in solution because of acidification of Bacterial medium, thereby inhibiting the formation of P-induced Pb precipitation. However, in the case of soil, Enterobacter sp. increased Pb immobilization by 6.98, 25.6 and 32.0% with the RP level of 200, 800 and 1600 mg P/kg, respectively. The immobilization of Pb in Pb-spiked soils was attributed to pyromorphite formation as indicated by XRD analysis. Inoculation of Phosphate Solubilizing Bacteria with RP in soil can be used as an alternative technique to soluble P compounds which can cause eutrophication of surface water.
-
isolation of Phosphate Solubilizing Bacteria and their potential for lead immobilization in soil
Journal of Hazardous Materials, 2011Co-Authors: Jin-hee Park, Nanthi Bolan, Mallavarapu Megharaj, Ravi NaiduAbstract:Abstract Lead (Pb), a highly toxic heavy metal forms stable compounds with Phosphate (P). The potential of Phosphate Solubilizing Bacteria (PSB) to immobilize Pb by enhancing solubilization of insoluble P compounds was tested in this research. Eighteen different PSB strains isolated from P amended and Pb contaminated soils were screened for their efficiency in P solubilization. The PSB isolated from P amended soils solubilized 217–479 mg/L of P while the PSB from Pb contaminated soil solubilized 31–293 mg/L of P. Stepwise multiple regression analysis and P solubility kinetics indicated that the major mechanism of P solubilization by PSB is the pH reduction through the release of organic acids. From the isolated Bacteria, two PSB were chosen for Pb immobilization and these Bacteria were identified as Pantoea sp. and Enterobacter sp., respectively. The PSB significantly increased P solubilization by 25.0% and 49.9% in the case of Pantoea sp., and 63.3% and 88.6% in the case of Enterobacter sp. for 200 and 800 mg/kg of rock Phosphate (RP) addition, respectively, thereby enhancing the immobilization of Pb by 8.25–13.7% in the case of Pantoea sp. and 14.7–26.4% in the case of Enterobacter sp. The ability of PSB to solubilize P, promote plant growth, and immobilize Pb can be used for phytostabilization of Pb contaminated soils.
Mallavarapu Megharaj - One of the best experts on this subject based on the ideXlab platform.
-
concomitant rock Phosphate dissolution and lead immobilization by Phosphate Solubilizing Bacteria enterobacter sp
Journal of Environmental Management, 2011Co-Authors: Jin-hee Park, Nanthi Bolan, Mallavarapu Megharaj, Ravi NaiduAbstract:Abstract This paper examines the potential value of Phosphate Solubilizing Bacteria (Enterobacter cloacae) in the dissolution of rock Phosphate (RP) and subsequent immobilization of lead (Pb) in both Bacterial growth medium and soils. Enterobacter sp. showed resistance to Pb and the bacterium solubilized 17.5% of RP in the growth medium. Entrobacter sp. did not enhance Pb immobilization in solution because of acidification of Bacterial medium, thereby inhibiting the formation of P-induced Pb precipitation. However, in the case of soil, Enterobacter sp. increased Pb immobilization by 6.98, 25.6 and 32.0% with the RP level of 200, 800 and 1600 mg P/kg, respectively. The immobilization of Pb in Pb-spiked soils was attributed to pyromorphite formation as indicated by XRD analysis. Inoculation of Phosphate Solubilizing Bacteria with RP in soil can be used as an alternative technique to soluble P compounds which can cause eutrophication of surface water.
-
concomitant rock Phosphate dissolution and lead immobilization by Phosphate Solubilizing Bacteria enterobacter sp
Journal of Environmental Management, 2011Co-Authors: Jin-hee Park, Nanthi Bolan, Mallavarapu Megharaj, Ravi NaiduAbstract:This paper examines the potential value of Phosphate Solubilizing Bacteria (Enterobacter cloacae) in the dissolution of rock Phosphate (RP) and subsequent immobilization of lead (Pb) in both Bacterial growth medium and soils. Enterobacter sp. showed resistance to Pb and the bacterium solubilized 17.5% of RP in the growth medium. Enterobacter sp. did not enhance Pb immobilization in solution because of acidification of Bacterial medium, thereby inhibiting the formation of P-induced Pb precipitation. However, in the case of soil, Enterobacter sp. increased Pb immobilization by 6.98, 25.6 and 32.0% with the RP level of 200, 800 and 1600 mg P/kg, respectively. The immobilization of Pb in Pb-spiked soils was attributed to pyromorphite formation as indicated by XRD analysis. Inoculation of Phosphate Solubilizing Bacteria with RP in soil can be used as an alternative technique to soluble P compounds which can cause eutrophication of surface water.
-
isolation of Phosphate Solubilizing Bacteria and their potential for lead immobilization in soil
Journal of Hazardous Materials, 2011Co-Authors: Jin-hee Park, Nanthi Bolan, Mallavarapu Megharaj, Ravi NaiduAbstract:Abstract Lead (Pb), a highly toxic heavy metal forms stable compounds with Phosphate (P). The potential of Phosphate Solubilizing Bacteria (PSB) to immobilize Pb by enhancing solubilization of insoluble P compounds was tested in this research. Eighteen different PSB strains isolated from P amended and Pb contaminated soils were screened for their efficiency in P solubilization. The PSB isolated from P amended soils solubilized 217–479 mg/L of P while the PSB from Pb contaminated soil solubilized 31–293 mg/L of P. Stepwise multiple regression analysis and P solubility kinetics indicated that the major mechanism of P solubilization by PSB is the pH reduction through the release of organic acids. From the isolated Bacteria, two PSB were chosen for Pb immobilization and these Bacteria were identified as Pantoea sp. and Enterobacter sp., respectively. The PSB significantly increased P solubilization by 25.0% and 49.9% in the case of Pantoea sp., and 63.3% and 88.6% in the case of Enterobacter sp. for 200 and 800 mg/kg of rock Phosphate (RP) addition, respectively, thereby enhancing the immobilization of Pb by 8.25–13.7% in the case of Pantoea sp. and 14.7–26.4% in the case of Enterobacter sp. The ability of PSB to solubilize P, promote plant growth, and immobilize Pb can be used for phytostabilization of Pb contaminated soils.
Nanthi Bolan - One of the best experts on this subject based on the ideXlab platform.
-
concomitant rock Phosphate dissolution and lead immobilization by Phosphate Solubilizing Bacteria enterobacter sp
Journal of Environmental Management, 2011Co-Authors: Jin-hee Park, Nanthi Bolan, Mallavarapu Megharaj, Ravi NaiduAbstract:Abstract This paper examines the potential value of Phosphate Solubilizing Bacteria (Enterobacter cloacae) in the dissolution of rock Phosphate (RP) and subsequent immobilization of lead (Pb) in both Bacterial growth medium and soils. Enterobacter sp. showed resistance to Pb and the bacterium solubilized 17.5% of RP in the growth medium. Entrobacter sp. did not enhance Pb immobilization in solution because of acidification of Bacterial medium, thereby inhibiting the formation of P-induced Pb precipitation. However, in the case of soil, Enterobacter sp. increased Pb immobilization by 6.98, 25.6 and 32.0% with the RP level of 200, 800 and 1600 mg P/kg, respectively. The immobilization of Pb in Pb-spiked soils was attributed to pyromorphite formation as indicated by XRD analysis. Inoculation of Phosphate Solubilizing Bacteria with RP in soil can be used as an alternative technique to soluble P compounds which can cause eutrophication of surface water.
-
concomitant rock Phosphate dissolution and lead immobilization by Phosphate Solubilizing Bacteria enterobacter sp
Journal of Environmental Management, 2011Co-Authors: Jin-hee Park, Nanthi Bolan, Mallavarapu Megharaj, Ravi NaiduAbstract:This paper examines the potential value of Phosphate Solubilizing Bacteria (Enterobacter cloacae) in the dissolution of rock Phosphate (RP) and subsequent immobilization of lead (Pb) in both Bacterial growth medium and soils. Enterobacter sp. showed resistance to Pb and the bacterium solubilized 17.5% of RP in the growth medium. Enterobacter sp. did not enhance Pb immobilization in solution because of acidification of Bacterial medium, thereby inhibiting the formation of P-induced Pb precipitation. However, in the case of soil, Enterobacter sp. increased Pb immobilization by 6.98, 25.6 and 32.0% with the RP level of 200, 800 and 1600 mg P/kg, respectively. The immobilization of Pb in Pb-spiked soils was attributed to pyromorphite formation as indicated by XRD analysis. Inoculation of Phosphate Solubilizing Bacteria with RP in soil can be used as an alternative technique to soluble P compounds which can cause eutrophication of surface water.
-
isolation of Phosphate Solubilizing Bacteria and their potential for lead immobilization in soil
Journal of Hazardous Materials, 2011Co-Authors: Jin-hee Park, Nanthi Bolan, Mallavarapu Megharaj, Ravi NaiduAbstract:Abstract Lead (Pb), a highly toxic heavy metal forms stable compounds with Phosphate (P). The potential of Phosphate Solubilizing Bacteria (PSB) to immobilize Pb by enhancing solubilization of insoluble P compounds was tested in this research. Eighteen different PSB strains isolated from P amended and Pb contaminated soils were screened for their efficiency in P solubilization. The PSB isolated from P amended soils solubilized 217–479 mg/L of P while the PSB from Pb contaminated soil solubilized 31–293 mg/L of P. Stepwise multiple regression analysis and P solubility kinetics indicated that the major mechanism of P solubilization by PSB is the pH reduction through the release of organic acids. From the isolated Bacteria, two PSB were chosen for Pb immobilization and these Bacteria were identified as Pantoea sp. and Enterobacter sp., respectively. The PSB significantly increased P solubilization by 25.0% and 49.9% in the case of Pantoea sp., and 63.3% and 88.6% in the case of Enterobacter sp. for 200 and 800 mg/kg of rock Phosphate (RP) addition, respectively, thereby enhancing the immobilization of Pb by 8.25–13.7% in the case of Pantoea sp. and 14.7–26.4% in the case of Enterobacter sp. The ability of PSB to solubilize P, promote plant growth, and immobilize Pb can be used for phytostabilization of Pb contaminated soils.
Bangxiao Zheng - One of the best experts on this subject based on the ideXlab platform.
-
d2o isotope labeling approach to probing Phosphate Solubilizing Bacteria in complex soil communities by single cell raman spectroscopy
Analytical Chemistry, 2019Co-Authors: Kai Yang, Bangxiao Zheng, Li CuiAbstract:Increasing the bioavailability of immobilized phosphorus (P) in soil by Phosphate-Solubilizing Bacteria (PSB) is an effective strategy for sustainable agronomic use of P and for mitigating the P cr...
-
D2O‑Isotope-Labeling Approach to Probing Phosphate-Solubilizing Bacteria in Complex Soil Communities by Single-Cell Raman Spectroscopy
2019Co-Authors: Kai Yang, Bangxiao Zheng, Li CuiAbstract:Increasing the bioavailability of immobilized phosphorus (P) in soil by Phosphate-Solubilizing Bacteria (PSB) is an effective strategy for sustainable agronomic use of P and for mitigating the P crisis. Here, D2O isotope labeling combined with single-cell Raman spectroscopy (Raman–D2O) was developed as an efficient activity-based approach to characterizing the presence and activity of PSB in a culture-independent way. On the basis of the finding that PSB were significantly more active than non-PSB in the presence of insoluble P, a C–D Raman band from active assimilation of D2O-derived D was established as a biomarker for both inorganic-Phosphate-Solubilizing Bacteria and organic-Phosphate-Solubilizing Bacteria. C–D ratios (intensities of C–D bands as percentages of the intensities of both the C–D and C–H bands) were further established as semiquantitative indicators of P-releasing activities because of the consistency between the C–D ratio and the concentration of solubilized Phosphate or acid phosphatase activity as measured by conventional bulk assays. By applying Raman imaging, single-cell Raman–D2O clearly discerned PSB in a mixed-soil Bacterial culture and even in complex soil communities. Remarkable heterogeneity of microbial activity, ranging from 2 to 30% (close to that in medium without P and that in medium with sufficient soluble P, respectively), was revealed at the single-cell level and clearly illustrated the subpopulation of soil Bacteria active in Solubilizing P. This work not only enables probing PSB and their P-releasing activities but also opens a window to explore more diverse microbial resources when obtaining related isotope-labeled substrates is prohibitive
-
identification and characterization of inorganic Phosphate Solubilizing Bacteria from agricultural fields with a rapid isolation method
AMB Express, 2018Co-Authors: Bangxiao Zheng, Kai Ding, Guowei Zhou, Muhammad Ibrahim, Dingpeng Zhang, Qingfang Bi, Hongzhe Li, Josep Penuelas, Xiaoru YangAbstract:The ability to solubilize fixed inorganic phosphorus (P) for plant growth is important for increasing crop yield. More P can be released by inoculating soil with inorganic-Phosphate-Solubilizing Bacteria (iPSBs). We used 96-well microplates instead of traditional 200-mm petri dishes to rapidly screen iPSB strains for their Solubilizing ability. We simultaneously obtained 76 iPSB isolates from 576 wells containing two agricultural soils. This method conveniently identified positive iPSB strains and effectively prevented fungal cross-contamination. Maximum-likelihood phylogenetic trees of the isolated strains showed that Bacillus megaterium was the most dominant iPSB, and strains Y99, Y95, Y924 and Y1412 were selected as representatives for the analysis of P solubilization. Succinic acid was the main organic acid of B. megaterium for releasing P. It was strongly correlated with the increase in soluble P concentration during 168 h of incubation of these four strains. pH was negatively exponentially correlated with the amount of soluble P in the medium, and the amount of succinic acid was strongly linearly correlated with the amount of P released (P < 0.001), suggesting that organic acid may mobilize microbial P. Our study provides an efficient and effective method for identifying and analyzing the growth of iPSB strains able to solubilize inorganic P and gives a better understanding of the mechanism of P solubilization.
-
long term nitrogen fertilization decreased the abundance of inorganic Phosphate Solubilizing Bacteria in an alkaline soil
Scientific Reports, 2017Co-Authors: Bangxiao Zheng, Kai Ding, Guowei Zhou, Qinglin Chen, Jiabao ZhangAbstract:Inorganic Phosphate Solubilizing Bacteria (iPSB) are essential to facilitate phosphorus (P) mobilization in alkaline soil, however, the phylogenetic structure of iPSB communities remains poorly characterized. Thus, we use a reference iPSB database to analyze the distribution of iPSB communities based on 16S rRNA gene illumina sequencing. Additionally, a noval pqqC primer was developed to quantify iPSB abundance. In our study, an alkaline soil with 27-year fertilization treatment was selected. The percentage of iPSB was 1.10~2.87% per sample, and the dominant iPSB genera were closely related to Arthrobacter, Bacillus, Brevibacterium and Streptomyces. Long-term P fertilization had no significant effect on the abundance of iPSB communities. Rather than P and potassium (K) additions, long-term nitrogen (N) fertilization decreased the iPSB abundance, which was validated by reduced relative abundance of pqqC gene (pqqC/16S). The decreased iPSB abundance was strongly related to pH decline and total N increase, revealing that the long-term N additions may cause pH decline and subsequent P releases relatively decreasing the demands of the iPSB community. The methodology and understanding obtained here provides insights into the ecology of inorganic P solubilizers and how to manipulate for better P use efficiency.