The Experts below are selected from a list of 37770 Experts worldwide ranked by ideXlab platform
Haiji Sun - One of the best experts on this subject based on the ideXlab platform.
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neurons and astrocytes in ventrolateral periaqueductal gray contribute to restraint water immersion stress induced gastric mucosal damage via the erk1 2 signaling pathway
The International Journal of Neuropsychopharmacology, 2021Co-Authors: Wenting Gao, Zepeng Wang, Hui Wang, Chenxu Huang, Yangyang Shen, Haiji SunAbstract:Background The restraint water immersion stress (RWIS) model includes both psychological and Physical Stimulation, which may lead to gastrointestinal disorders and cause gastric mucosal damage. The ventrolateral periaqueductal gray (VLPAG) contributes to gastrointestinal function, but whether it is involved in RWIS-induced gastric mucosal damage has not yet been reported. Methods The expression of glial fibrillary acidic protein (GFAP), neuronal c-Fos and phosphorylated extracellular signal regulated kinase 1/2 (p-ERK1/2) in the VLPAG after RWIS was assessed using western blotting and immunocytochemical staining methods. Lateral ventricle injection of astrocytic toxin L-a-aminoadipate (L-AA) and treatment with ERK1/2 signaling pathway inhibitor PD98059 were further used to study protein expression and distribution in the VLPAG after RWIS. Results The expression of c-Fos, GFAP, and p-ERK1/2 in the VLPAG significantly increased following RWIS and peaked at 1 h after RWIS. Lateral ventricle injection of the astrocytic toxin L-AA significantly alleviated gastric mucosal injury and decreased the activation of neurons and astrocytes. Treatment with the ERK1/2 signaling pathway inhibitor PD98059 obviously suppressed gastric mucosal damage as well as the RWIS-induced activation of neurons and astrocytes in the VLPAG. Conclusions These results suggested that activation of VLPAG neurons and astrocytes induced by RWIS through the ERK1/2 signaling pathway may play a critical role in RWIS-induced gastric mucosa damage.
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effects of restraint water immersion stress induced gastric mucosal damage on astrocytes and neurons in the nucleus raphe magnus of rats via the erk1 2 signaling pathway
Neurochemical Research, 2019Co-Authors: Fangcheng Fan, Mengzhu Yang, Xiwen Geng, Haiji SunAbstract:Restraint water-immersion stress (RWIS) consists of psychological and Physical Stimulation, and it has been utilized in the research of gastric mucosal damage. It has been shown by previous studies that the nucleus raphe magnus (NRM) is closely involved in the gastrointestinal function, but its functions on the stress-induced gastric mucosal injury (SGMI) have not been thoroughly elucidated to date. Consequently, in this research, we aim to measure the expression of astrocytic glial fibrillary acidic protein (GFAP), neuronal c-Fos, and phosphorylation extracellular signal regulated kinase 1/2 (p-ERK1/2) in the process of RWIS with immunohistochemistry and western blot methods. What is more, we detect the relation between astrocytes and neurons throughout the stress procedure and explore the regulation of the ERK1/2 signaling pathway on the activity of astrocytes and neurons after RWIS. The results indicated that all three proteins expression multiplied following peaked 3 h substantially. The SMGI, astrocyte and neuron activity were affected after the astrocytotoxin L-A-aminohexanedioic acid (L-AA) and c-fos antisense oligonucleotide (ASO) injections. After the injection of PD98059, the gastric mucosal injury, astrocyte and neuron activity significantly fell off. These results suggested that RWIS-induced activity of astrocytes and neurons in the NRM may play a significant part in gastric mucosa damage via the ERK1/2 signaling pathway.
Jinwoo Cheon - One of the best experts on this subject based on the ideXlab platform.
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Non-contact long-range magnetic Stimulation of mechanosensitive ion channels in freely moving animals
Nature Materials, 2021Co-Authors: Jung-uk Lee, Wookjin Shin, Yongjun Lim, Jungsil Kim, Woon Ryoung Kim, Heehun Kim, Jae-hyun Lee, Jinwoo CheonAbstract:A magnetic torque actuator has been developed and is capable of modulation of neurons expressing the mechanosensitive ion channel, Piezo1, resulting in long-distance control of locomotion of mice. Among Physical Stimulation modalities, magnetism has clear advantages, such as deep penetration and untethered interventions in biological subjects. However, some of the working principles and effectiveness of existing magnetic neuroStimulation approaches have been challenged, leaving questions to be answered. Here we introduce m-Torquer, a magnetic toolkit that mimics magnetoreception in nature. It comprises a nanoscale magnetic torque actuator and a circular magnet array, which deliver piconewton-scale forces to cells over a working range of ~70 cm. With m-Torquer, Stimulation of neurons expressing bona fide mechanosensitive ion channel Piezo1 enables consistent and reproducible neuromodulation in freely moving mice. With its long working distance and cellular targeting capability, m-Torquer provides versatility in its use, which can range from single cells to in vivo systems, with the potential application in large animals such as primates.
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Non-contact long-range magnetic Stimulation of mechanosensitive ion channels in freely moving animals
'Springer Science and Business Media LLC', 2021Co-Authors: Jung-uk Lee, Wookjin Shin, Yongjun Lim, Jungsil Kim, Woon Ryoung Kim, Heehun Kim, Jae-hyun Lee, Jinwoo CheonAbstract:Among Physical Stimulation modalities, magnetism has clear advantages, such as deep penetration and untethered interventions in biological subjects. However, some of the working principles and effectiveness of existing magnetic neuroStimulation approaches have been challenged, leaving questions to be answered. Here we introduce m-Torquer, a magnetic toolkit that mimics magnetoreception in nature. It comprises a nanoscale magnetic torque actuator and a circular magnet array, which deliver piconewton-scale forces to cells over a working range of similar to 70 cm. With m-Torquer, Stimulation of neurons expressing bona fide mechanosensitive ion channel Piezo1 enables consistent and reproducible neuromodulation in freely moving mice. With its long working distance and cellular targeting capability, m-Torquer provides versatility in its use, which can range from single cells to in vivo systems, with the potential application in large animals such as primates.11Nsciescopu
Fangcheng Fan - One of the best experts on this subject based on the ideXlab platform.
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effects of restraint water immersion stress induced gastric mucosal damage on astrocytes and neurons in the nucleus raphe magnus of rats via the erk1 2 signaling pathway
Neurochemical Research, 2019Co-Authors: Fangcheng Fan, Mengzhu Yang, Xiwen Geng, Haiji SunAbstract:Restraint water-immersion stress (RWIS) consists of psychological and Physical Stimulation, and it has been utilized in the research of gastric mucosal damage. It has been shown by previous studies that the nucleus raphe magnus (NRM) is closely involved in the gastrointestinal function, but its functions on the stress-induced gastric mucosal injury (SGMI) have not been thoroughly elucidated to date. Consequently, in this research, we aim to measure the expression of astrocytic glial fibrillary acidic protein (GFAP), neuronal c-Fos, and phosphorylation extracellular signal regulated kinase 1/2 (p-ERK1/2) in the process of RWIS with immunohistochemistry and western blot methods. What is more, we detect the relation between astrocytes and neurons throughout the stress procedure and explore the regulation of the ERK1/2 signaling pathway on the activity of astrocytes and neurons after RWIS. The results indicated that all three proteins expression multiplied following peaked 3 h substantially. The SMGI, astrocyte and neuron activity were affected after the astrocytotoxin L-A-aminohexanedioic acid (L-AA) and c-fos antisense oligonucleotide (ASO) injections. After the injection of PD98059, the gastric mucosal injury, astrocyte and neuron activity significantly fell off. These results suggested that RWIS-induced activity of astrocytes and neurons in the NRM may play a significant part in gastric mucosa damage via the ERK1/2 signaling pathway.
Wenting Gao - One of the best experts on this subject based on the ideXlab platform.
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neurons and astrocytes in ventrolateral periaqueductal gray contribute to restraint water immersion stress induced gastric mucosal damage via the erk1 2 signaling pathway
The International Journal of Neuropsychopharmacology, 2021Co-Authors: Wenting Gao, Zepeng Wang, Hui Wang, Chenxu Huang, Yangyang Shen, Haiji SunAbstract:Background The restraint water immersion stress (RWIS) model includes both psychological and Physical Stimulation, which may lead to gastrointestinal disorders and cause gastric mucosal damage. The ventrolateral periaqueductal gray (VLPAG) contributes to gastrointestinal function, but whether it is involved in RWIS-induced gastric mucosal damage has not yet been reported. Methods The expression of glial fibrillary acidic protein (GFAP), neuronal c-Fos and phosphorylated extracellular signal regulated kinase 1/2 (p-ERK1/2) in the VLPAG after RWIS was assessed using western blotting and immunocytochemical staining methods. Lateral ventricle injection of astrocytic toxin L-a-aminoadipate (L-AA) and treatment with ERK1/2 signaling pathway inhibitor PD98059 were further used to study protein expression and distribution in the VLPAG after RWIS. Results The expression of c-Fos, GFAP, and p-ERK1/2 in the VLPAG significantly increased following RWIS and peaked at 1 h after RWIS. Lateral ventricle injection of the astrocytic toxin L-AA significantly alleviated gastric mucosal injury and decreased the activation of neurons and astrocytes. Treatment with the ERK1/2 signaling pathway inhibitor PD98059 obviously suppressed gastric mucosal damage as well as the RWIS-induced activation of neurons and astrocytes in the VLPAG. Conclusions These results suggested that activation of VLPAG neurons and astrocytes induced by RWIS through the ERK1/2 signaling pathway may play a critical role in RWIS-induced gastric mucosa damage.
Xiwen Geng - One of the best experts on this subject based on the ideXlab platform.
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effects of restraint water immersion stress induced gastric mucosal damage on astrocytes and neurons in the nucleus raphe magnus of rats via the erk1 2 signaling pathway
Neurochemical Research, 2019Co-Authors: Fangcheng Fan, Mengzhu Yang, Xiwen Geng, Haiji SunAbstract:Restraint water-immersion stress (RWIS) consists of psychological and Physical Stimulation, and it has been utilized in the research of gastric mucosal damage. It has been shown by previous studies that the nucleus raphe magnus (NRM) is closely involved in the gastrointestinal function, but its functions on the stress-induced gastric mucosal injury (SGMI) have not been thoroughly elucidated to date. Consequently, in this research, we aim to measure the expression of astrocytic glial fibrillary acidic protein (GFAP), neuronal c-Fos, and phosphorylation extracellular signal regulated kinase 1/2 (p-ERK1/2) in the process of RWIS with immunohistochemistry and western blot methods. What is more, we detect the relation between astrocytes and neurons throughout the stress procedure and explore the regulation of the ERK1/2 signaling pathway on the activity of astrocytes and neurons after RWIS. The results indicated that all three proteins expression multiplied following peaked 3 h substantially. The SMGI, astrocyte and neuron activity were affected after the astrocytotoxin L-A-aminohexanedioic acid (L-AA) and c-fos antisense oligonucleotide (ASO) injections. After the injection of PD98059, the gastric mucosal injury, astrocyte and neuron activity significantly fell off. These results suggested that RWIS-induced activity of astrocytes and neurons in the NRM may play a significant part in gastric mucosa damage via the ERK1/2 signaling pathway.