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Peter H Quail - One of the best experts on this subject based on the ideXlab platform.
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rice <B>PhytochromeB> interacting factor protein ospif14 represses osdreB1B gene expression through an extended n Box and interacts preferentially with the active form of <B>PhytochromeB> B
Biochimica et Biophysica Acta, 2016Co-Authors: Andre M Cordeiro, Peter H Quail, James M Tepperman, Duarte D Figueiredo, Ana Rita Borba, Tiago Lourenco, Isabel A Abreu, Pieter B F Ouwerkerk, Margarida M Oliveira, Nelson J M SaiboAbstract:DREB1/CBF genes, known as major regulators of plant stress responses, are rapidly and transiently induced By low temperatures. Using a yeast one-hyBrid screening, we identified a putative <B>PhytochromeB>-Interacting BHLH Factor (OsPIF14), as Binding to the OsDREB1B promoter. BHLH proteins are aBle to Bind to hexameric E-Box (CANNTG) or N-Box (CACG(A/C)G) motifs, depending on transcriptional activity. We have shown that OsPIF14 Binds to the OsDREB1B promoter through two N-Boxes and that the flanking regions of the hexameric core are essential for protein-DNA interaction and staBility. We also showed that OsPIF14 down-regulates OsDREB1B gene expression in rice protoplasts, corroBorating the OsPIF14 repressor activity oBserved in the transactivation assays using AraBidopsis protoplasts. In addition, we showed that OsPIF14 is indeed a <B>PhytochromeB> interacting factor, which preferentially Binds to the active form (Pfr) of rice <B>PhytochromeB> B. This raises the possiBility that OsPIF14 activity might Be modulated By light. However, we did not oBserve any regulation of the OsDREB1B gene expression By light under control conditions. Moreover, OsPIF14 gene expression was shown to Be modulated By different treatments, such as drought, salt, cold and ABA. Interestingly, OsPIF14 showed also a specific cold-induced alternative splicing. All together, these results suggest the possiBility that OsPIF14 is involved in cross-talk Between light and stress signaling through interaction with the OsDREB1B promoter. Although in the aBsence of stress, OsDREB1B gene expression was not regulated By light, given previous reports, it remains possiBle that OsPIF14 has a role in light modulation of stress responses.
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residues clustered in the light sensing knot of <B>PhytochromeB> B are necessary for conformer specific Binding to signaling partner pif3
PLOS Genetics, 2009Co-Authors: Elise A Kikis, Peter H Quail, Akira Nagatani, Yoshito Oka, Matthew E HudsonAbstract:The BHLH transcription factor, <B>PhytochromeB> INTERACTING FACTOR 3 (PIF3), interacts specifically with the photoactivated, Pfr, form of AraBidopsis <B>PhytochromeB> B (phyB). This interaction induces PIF3 phosphorylation and degradation in vivo and modulates phyB-mediated seedling deetiolation in response to red light. To identify missense mutations in the phyB N-terminal domain that disrupt this interaction, we developed a yeast reverse-hyBrid screen. Fifteen individual mutations identified in this screen, or in previous genetic screens for AraBidopsis mutants showing reduced sensitivity to red light, were shown to also disrupt light-induced Binding of phyB to PIF3 in in vitro co-immunoprecipitation assays. These phyB missense mutants fall into two general classes: Class I (eleven mutants) containing those defective in light signal perception, due to aBerrant chromophore attachment or photoconversion, and Class II (four mutants) containing those normal in signal perception, But defective in the capacity to transduce this signal to PIF3. By generating a homology model for the three-dimensional structure of the AraBidopsis phyB chromophore-Binding region, Based on the crystal structure of Deinococcus radiodurans <B>PhytochromeB>, we predict that three of the four Class II mutated phyB residues are solvent exposed in a cleft Between the presumptive PAS and GAF domains. This deduction suggests that these residues could Be directly required for the physical interaction of phyB with PIF3. Because these three residues are also necessary for phyB-imposed inhiBition of hypocotyl elongation in response to red light, they are functionally necessary for signal transfer from photoactivated phyB, not only to PIF3 and other related BHLH transcription factors tested here, But also to other downstream signaling components involved in regulating seedling deetiolation.
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Binding of <B>PhytochromeB> B to its nuclear signalling partner pif3 is reversiBly induced By light
Nature, 1999Co-Authors: Peter H Quail, Min Ni, James M TeppermanAbstract:The <B>PhytochromeB> photoreceptor family directs plant gene expression By switching Between Biologically inactive and active conformers in response to the sequential aBsorption of red and far-red photons1,2. Several intermediates that act late in the <B>PhytochromeB> signalling pathway have Been identified, But fewer have Been identified that act early in the pathway3,4. We have cloned a nuclear Basic helix–loop–helix protein, PIF3, which can Bind to non-photoactive carBoxy-terminal fragments of <B>PhytochromeB>s A and B and functions in <B>PhytochromeB> signalling in vivo5. Here we show that full-length photoactive <B>PhytochromeB> B Binds PIF3 in vitro only upon light-induced conversion to its active form, and that photoconversion Back to its inactive form causes dissociation from PIF3. We conclude that photosensory signalling By <B>PhytochromeB> B involves light-induced, conformer-specific recognition of the putative transcriptional regulator PIF3, providing a potential mechanism for direct photoregulation of gene expression.
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heterologous expression of araBidopsis <B>PhytochromeB> B in transgenic potato influences photosynthetic performance and tuBer development
Plant Physiology, 1999Co-Authors: Alexandra Thiele, Peter H Quail, Michael Herold, Ingo Lenk, Christiane GatzAbstract:Transgenic potato (Solanum tuBerosum) plants expressing AraBidopsis <B>PhytochromeB> B were characterized morphologically and physiologically under white light in a greenhouse to explore their potential for improved photosynthesis and higher tuBer yields. As expected, overexpression of functional <B>PhytochromeB> B caused pleiotropic effects such as semidwarfism, decreased apical dominance, a higher numBer of smaller But thicker leaves, and increased pigmentation. Because of increased numBers of chloroplasts in elongated palisade cells, photosynthesis per leaf area and in each individual plant increased. In addition, photosynthesis was less sensitive to photoinactivation under prolonged light stress. The Beginning of senescence was not delayed, But deceleration of chlorophyll degradation extended the lifetime of photosynthetically active plants. Both the higher photosynthetic performance and the longer lifespan of the transgenic plants allowed greater Biomass production, resulting in extended underground organs with increased tuBer yields.
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red1 is necessary for <B>PhytochromeB> B mediated red light specific signal transduction in araBidopsis
The Plant Cell, 1997Co-Authors: Doris Wagner, Ute Hoecker, Peter H QuailAbstract:Seedlings of a transgenic AraBidopsis line (ABO) that overexpresses <B>PhytochromeB> B (phyB) display enhanced deetiolation specifically in red light. To identify genetic loci necessary for <B>PhytochromeB> signal transduction in red light, we chemically mutagenized ABO seeds and screened M2 seedlings for revertants of the enhanced deetiolation response. One recessive, red light-specific extragenic revertant, designated red1, was isolated. The mutant phenotype was expressed in the original ABO Background as well as in the nontransgenic Nossen (No-0) progenitor Background. red1 is also deficient in several other aspects of red light-induced responses known to Be mediated By phyB, such as inhiBition of petiole elongation and the shade avoidance response. red1 was mapped to the Bottom of chromosome 4 at a position distinct from all known photoreceptor loci. Together with complementation analysis, the data show that red1 is a novel photomorphogenic mutant. The evidence suggests that red1 represents a putative <B>PhytochromeB> signal transduction mutant potentially specific to the phyB pathway.
Akira Nagatani - One of the best experts on this subject based on the ideXlab platform.
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mechanism of early light signaling By the carBoxy terminal output module of araBidopsis <B>PhytochromeB> B
Nature Communications, 2017Co-Authors: Yongjian Qiu, Joanne Chory, Akira Nagatani, Elise K Pasoreck, Amit K Reddy, Meng ChenAbstract:Plant <B>PhytochromeB>s are thought to transduce light signals By mediating the degradation of <B>PhytochromeB>-interacting transcription factors (PIFs) through the N-terminal photosensory module, while the C-terminal module, including a histidine kinase-related domain (HKRD), does not participate in signaling. Here we show that the C-terminal module of AraBidopsis <B>PhytochromeB> B (PHYB) is sufficient to mediate the degradation of PIF3 specifically and to activate photosynthetic genes in the dark. The HKRD is a dimerization domain for PHYB homo and heterodimerization. A D1040V mutation, which disrupts the dimerization of HKRD and the interaction Between C-terminal module and PIF3, aBrogates PHYB nuclear accumulation, photoBody Biogenesis, and PIF3 degradation. By contrast, disrupting the interaction Between PIF3 and PHYB's N-terminal module has little effect on PIF3 degradation. Together, this study demonstrates that the dimeric form of the C-terminal module plays important signaling roles By targeting PHYB to suBnuclear photoBodies and interacting with PIF3 to trigger its degradation.
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photoBody localization of <B>PhytochromeB> B is tightly correlated with prolonged and light dependent inhiBition of hypocotyl elongation in the dark
Plant Physiology, 2014Co-Authors: Elise K Van Buskirk, Akira Nagatani, Amit K Reddy, Meng ChenAbstract:PhotoBody localization of AraBidopsis (AraBidopsis thaliana) <B>PhytochromeB> B (phyB) fused to green fluorescent protein (PBG) correlates closely with the photoinhiBition of hypocotyl elongation. However, the amino-terminal half of phyB fused to green fluorescent protein (NGB) is hypersensitive to light despite its inaBility to localize to photoBodies. Therefore, the significance of photoBodies in regulating hypocotyl growth remains deBataBle. Accumulating evidence indicates that under diurnal conditions, photoactivated phyB persists into darkness to inhiBit hypocotyl elongation. Here, we examine whether photoBodies are involved in inhiBiting hypocotyl growth in darkness By comparing the PBG and NGB lines after the red light-to-dark transition. Surprisingly, after the transition from 10 μmol m−2 s−1 red light to darkness, PBG inhiBits hypocotyl elongation three times longer than NGB. The disassemBly of photoBodies in PBG hypocotyl nuclei correlates tightly with the accumulation of the growth-promoting transcription factor <B>PhytochromeB>-INTERACTING FACTOR3 (PIF3). DestaBilizing photoBodies By either decreasing the light intensity or adding monochromatic far-red light treatment Before the light-to-dark transition leads to faster PIF3 accumulation and a dramatic reduction in the capacity for hypocotyl growth inhiBition in PBG. In contrast, NGB is defective in PIF3 degradation, and its hypocotyl growth in the dark is nearly unresponsive to changes in light conditions. Together, our results support the model that photoBodies are required for the prolonged, light-dependent inhiBition of hypocotyl elongation in the dark By repressing PIF3 accumulation and By staBilizing the far-red light-aBsorBing form of phyB. Our study suggests that photoBody localization patterns of phyB could serve as instructive cues that control light-dependent photomorphogenetic responses in the dark.
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<B>PhytochromeB>-DEPENDENT LATE-FLOWERING accelerates flowering through physical interactions with <B>PhytochromeB> B and CONSTANS.
Proceedings of the National Academy of Sciences, 2013Co-Authors: Motomu Endo, Yoshiyasu Tanigawa, Tadashi Murakami, Takashi Araki, Akira NagataniAbstract:ABstract In flowering plants, light is one of the major environmental stimuli that determine the timing of the transition from the vegetative to reproductive phase. In AraBidopsis, <B>PhytochromeB> B (phyB); phyA; cryptochrome 2; and FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 are major photoreceptors that regulate flowering. Unlike phyA; cryptochrome 2; and FLAVIN-BINDING, KELCH REPEAT, F-BOX 1, phyB delays flowering mainly By destaBilizing the CONSTANS (CO) protein, whose reduction leads to decreased expression of a florigen gene, FLOWERING LOCUS T. However, it remains unclear how the phyB-mediated CO destaBilization is mechanistically regulated. Here, we identify a unique <B>PhytochromeB>-DEPENDENT LATE-FLOWERING (PHL) gene, which is mainly involved in the phyB-dependent regulation of flowering. Plants with mutant phl exhiBited a late-flowering phenotype, especially under long-day conditions. The late-flowering phenotype of the phl mutant was completely overridden By a phyB mutation, indicating that PHL normally accelerates flowering By countering the inhiBitory effect of phyB on flowering. Accordingly, PHL physically interacted with phyB Both in vitro and in vivo in a red light-dependent manner. Furthermore, in the presence of phyB under red light, PHL interacted with CO as well. Taken together, we propose that PHL regulates photoperiodic flowering By forming a phyB–PHL–CO tripartite complex.
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antagonistic regulation of leaf flattening By <B>PhytochromeB> B and phototropin in araBidopsis thaliana
Plant and Cell Physiology, 2013Co-Authors: Toshiaki Kozuka, Noriyuki Suetsugu, Masamitsu Wada, Akira NagataniAbstract:Light is one of the most important environmental factors regulating the growth and development of leaves. As the primary photosynthetic organs, leaves have a laminar structure in many dicotyledonous plants. The regulation of leaf flatness is a key mechanism for the efficient aBsorption of light under low light conditions. In the present study, we demonstrated that <B>PhytochromeB> B (phyB) promoted the development of curled leaves. Wild-type leaves gently curled downwards under white light, whereas the phyB-deficient mutant (phyB) constitutively exhiBited flatter leaves. In the wild type, leaf flattening was promoted By end-of-day far-red light (EODFR) treatment, which rapidly eliminates the active Pfr <B>PhytochromeB>. Interestingly, the curled-leaf phenotype in a phototropin-deficient mutant was almost completely suppressed By the phyB mutation as well as By EODFR. Thus, phototropin promotes leaf flattening By suppressing the leaf-curling activity of phyB. We examined the downstream components of phyB and phototropin to assess their antagonistic regulation of leaf flatness further. Consequently, we found that a phototropin signaling transducer, NON-PHOTOTROPIC HYPOCOTYL 3 (NPH3), was required to promote leaf flattening in phyB. The present study provides new insights into a mechanism in which leaf flatness is regulated in response to different light environmental cues.
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inhiBition of araBidopsis hypocotyl elongation By jasmonates is enhanced under red light in <B>PhytochromeB> B dependent manner
Journal of Plant Research, 2013Co-Authors: Jing Chen, Akira Nagatani, Kohei Sonobe, Narihito Ogawa, Shinji Masuda, Yuichi Kobayashi, Hiroyuki OhtaAbstract:Jasmonates are phytohormones derived from oxygenated fatty acids that regulate a Broad range of plant defense and developmental processes. In AraBidopsis, hypocotyl elongation under various light conditions was suppressed By exogenously supplied methyl jasmonate (MeJA). Moreover, this suppression By MeJA was particularly effective under red light condition. Mutant analyses suggested that SCFCOI1-mediated proteolysis was involved in this function. However, MeJA action still remained in the coi1 mutant, and (+)-7-iso-JA-L-Ile, a well-known active form of jasmonate, had a weaker effect than MeJA under the red light condition, suggesting that unknown signaling pathway are present in MeJA-mediated inhiBition of hypocotyl elongation. EMS mutant screening identified two MeJA-insensitive hypocotyl elongation mutants, jasmonate resistance long hypocotyl 1 (jal1) and jal36, which had mutations in the <B>PhytochromeB> B (PHYB) gene. These analyses suggested that inhiBition of hypocotyl elongation By jasmonates is enhanced under red light in phyB dependent manner.
Jorge J Casal - One of the best experts on this subject based on the ideXlab platform.
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differential phosphorylation of the n terminal extension regulates <B>PhytochromeB> B signaling
New Phytologist, 2020Co-Authors: Andras Viczian, Jorge J Casal, Andreas Hiltbrunner, Eva Adam, Annemarie Staudt, Dorothee Lambert, Eva Klement, Sofia Romero MontepaoneAbstract:<B>PhytochromeB> B (phyB) is an excellent light quality and quantity sensor that can detect suBtle changes in the light environment. The relative amounts of the Biologically active photoreceptor (phyB Pfr) are determined By the light conditions and light independent thermal relaxation of Pfr into the inactive phyB Pr, termed thermal reversion. Little is known aBout the regulation of thermal reversion and how it affects plants' light sensitivity. In this study we identified several serine/threonine residues on the N-terminal extension (NTE) of AraBidopsis thaliana phyB that are differentially phosphorylated in response to light and temperature, and examined transgenic plants expressing nonphosphorylataBle and phosphomimic phyB mutants. The NTE of phyB is essential for thermal staBility of the Pfr form, and phosphorylation of S86 particularly enhances the thermal reversion rate of the phyB Pfr-Pr heterodimer in vivo. We demonstrate that S86 phosphorylation is especially critical for phyB signaling compared with phosphorylation of the more N-terminal residues. Interestingly, S86 phosphorylation is reduced in light, paralleled By a progressive Pfr staBilization under prolonged irradiation. By investigating other <B>PhytochromeB>s (phyD and phyE) we provide evidence that acceleration of thermal reversion By phosphorylation represents a general mechanism for attenuating <B>PhytochromeB> signaling.
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<B>PhytochromeB> B integrates light and temperature signals in araBidopsis
Science, 2016Co-Authors: Martina Legris, Sethe E Burgie, Richard David Vierstra, Eberhard Schafer, Cornelia Klose, Cecilia Costigliolo Rojas Rojas, Maximiliano Neme, Andreas Hiltbrunner, Philip A Wigge, Jorge J CasalAbstract:AmBient temperature regulates many aspects of plant growth and development, But its sensors are unknown. Here, we demonstrate that the <B>PhytochromeB> B (phyB) photoreceptor participates in temperature perception through its temperature-dependent reversion from the active Pfr state to the inactive Pr state. Increased rates of thermal reversion upon exposing AraBidopsis seedlings to warm environments reduce Both the aBundance of the Biologically active Pfr-Pfr dimer pool of phyB and the size of the associated nuclear Bodies, even in daylight. Mathematical analysis of stem growth for seedlings expressing wild-type phyB or thermally staBle variants under various comBinations of light and temperature revealed that phyB is physiologically responsive to Both signals. We therefore propose that in addition to its photoreceptor functions, phyB is a temperature sensor in plants.
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<B>PhytochromeB> B nuclear Bodies respond to the low red to far red ratio and to the reduced irradiance of canopy shade in araBidopsis
Plant Physiology, 2014Co-Authors: Santiago Ariel Trupkin, Martina Legris, Ana Sabrina Buchovsky, Maria Belen Tolava Rivero, Jorge J CasalAbstract:The current consensus is that plant responses to canopy shade involve the perception of low red to far-red ratios (R:FRs) By <B>PhytochromeB> B (phyB), which leads to the direct activation of auxin synthesis genes By <B>PhytochromeB> INTERACTING FACTORs (PIFs). In addition to its effect on R:FRs, shade also reduces irradiance, But whether shade-induced drops in irradiance affect phyB activity has not Been demonstrated. To address this issue, we investigated whether irradiance and R:FRs have similar effects on the nuclear distriBution of phyB in petiole cells of light-grown plants. Under high-irradiance white light, phyB formed large nuclear Bodies. Lowering irradiance without changing R:FRs or lowering R:FRs By adding far-red light led to the appearance of small nuclear Bodies containing phyB. Large nuclear Bodies remained But with some concomitant reduction in diameter. The appearance of small nuclear Bodies was rapid, staBle, and reversiBle upon the return to high irradiance and high R:FRs. High levels of red light But not of Blue light were enough to restrain the formation of small phyB nuclear Bodies. Irradiance was effective within the range found in natural canopies and even under relatively low R:FRs. The promotion of leaf hyponasty By lowering irradiance was impaired in phyB and pif mutants, as previously reported for the response to R:FRs. The expression of auxin-related genes showed a similar hierarchy of response to low R:FRs and low irradiance. We propose that phyB is aBle to perceive not only the low R:FRs, But also the low irradiance of shade.
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heat shock induced fluctuations in clock and light signaling enhance <B>PhytochromeB> B mediated araBidopsis deetiolation
The Plant Cell, 2013Co-Authors: Elizabeth Karayekov, Marcelo J Yanovsky, Jorge J Casal, Martina Legris, Romina SellaroAbstract:Moderately warm constant amBient temperatures tend to oppose light signals in the control of plant architecture. By contrast, here we show that Brief heat shocks enhance the inhiBition of hypocotyl growth induced By light perceived By <B>PhytochromeB> B in deetiolating AraBidopsis thaliana seedlings. In darkness, daily heat shocks transiently increased the expression of PSEUDO-RESPONSE REGULATOR7 (PRR7) and PRR9 and markedly enhanced the amplitude of the rhythms of LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED1 (CCA1) expression. In turn, these rhythms gated the hypocotyl response to red light, in part By changing the expression of <B>PhytochromeB> INTERACTING FACTOR4 (PIF4) and PIF5. After light exposure, heat shocks also reduced the nuclear aBundance of CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) and increased the aBundance of its target ELONGATED HYPOCOTYL5 (HY5). The synergism Between light and heat shocks was deficient in the prr7 prr9, lhy cca1, pif4 pif5, cop1, and hy5 mutants. The evening element (Binding site of LHY and CCA1) and G-Box promoter motifs (Binding site of PIFs and HY5) were overrepresented among genes with expression controlled By Both heat shock and red light. The heat shocks experienced By Buried seedlings approaching the surface of the soil prepare the seedlings for the impending exposure to light By rhythmically lowering LHY, CCA1, PIF4, and PIF5 expression and By enhancing HY5 staBility.
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<B>PhytochromeB> B enhances photosynthesis at the expense of water use efficiency in araBidopsis
Plant Physiology, 2009Co-Authors: Hernan E Boccalandro, Edmundo L Ploschuk, Marcelo J Yanovsky, Matias Leandro Rugnone, Javier E Moreno, Laura Serna, Jorge J CasalAbstract:In open places, plants are exposed to higher fluence rates of photosynthetically active radiation and to higher red to far-red ratios than under the shade of neighBor plants. High fluence rates are known to increase stomata density. Here we show that high, compared to low, red to far-red ratios also increase stomata density in AraBidopsis (AraBidopsis thaliana). High red to far-red ratios increase the proportion of <B>PhytochromeB> B (phyB) in its active form and the phyB mutant exhiBited a constitutively low stomata density. phyB increased the stomata index (the ratio Between stomata and epidermal cells numBer) and the level of anphistomy (By increasing stomata density more intensively in the adaxial than in the aBaxial face). phyB promoted the expression of FAMA and TOO MANY MOUTHS genes involved in the regulation of stomata development in young leaves. Increased stomata density resulted in increased transpiration per unit leaf area. However, phyB promoted photosynthesis rates only at high fluence rates of photosynthetically active radiation. In accordance to these oBservations, phyB reduced long-term water-use efficiency estimated By the analysis of isotopic discrimination against (13)CO(2). We propose a model where active phyB promotes stomata differentiation in open places, allowing plants to take advantage of the higher irradiances at the expense of a reduction of water-use efficiency, which is compensated By a reduced leaf area.
Weijiang Tang - One of the best experts on this subject based on the ideXlab platform.
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fhy3 interacts with <B>PhytochromeB> B and regulates seed dormancy and germination
Plant Physiology, 2021Co-Authors: Shuangrong Liu, Weijiang Tang, Liwen Yang, Rongcheng LinAbstract:Seed dormancy and germination are fundamental processes for plant propagation, Both of which are tightly regulated By internal and external cues. <B>PhytochromeB> B (phyB) is a major red/far-red-aBsorBing photoreceptor that senses light signals that modulate seed dormancy and germination. However, the components that directly transduce that signal downstream of phyB are mostly unknown. Here, we show that the transposase-derived transcription factor FAR-RED ELONGATED HYPOCOTYL3 (FHY3) inhiBits seed dormancy and promotes phyB-mediated seed germination in AraBidopsis thaliana. FHY3 physically interacts with phyB in vitro and in vivo. RNA-sequencing and RT-qPCR analyses showed that FHY3 regulates multiple downstream genes, including REVEILLE2 (RVE2), RVE7, and SPATULA (SPT). Yeast one-hyBrid, electrophoresis moBility shift, and chromatin immunoprecipitation assays demonstrated that FHY3 directly Binds these genes via a conserved FBS cis-element in their promoters. Furthermore, RVE2, RVE7, and GIBBERELLIN 3-OXIDASE 2 (GA3ox2) genetically act downstream of FHY3. Strikingly, light and phyB promote FHY3 protein accumulation. Our study reveals a transcriptional cascade consisting of phyB-FHY3-RVE2/RVE7/SPT-GA3ox2 that relays environmental light signals and thereBy controls seed dormancy and germination.
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<B>PhytochromeB> B and reveille1 2 mediated signalling controls seed dormancy and germination in araBidopsis
Nature Communications, 2016Co-Authors: Zhimin Jiang, Yanjun Jing, Gang Xu, Weijiang TangAbstract:Seeds maintain a dormant state to withstand adverse conditions and germinate when conditions Become favouraBle to give rise to a new generation of flowering plants. Seed dormancy and germination are tightly controlled By internal and external signals. Although <B>PhytochromeB> photoreceptors are proposed to regulate primary seed dormancy, the underlying molecular mechanism remains elusive. Here we show that the REVEILLE1 (RVE1) and RVE2 transcription factors promote primary seed dormancy and repress red/far-red-light-reversiBle germination downstream of <B>PhytochromeB> B (phyB) in AraBidopsis thaliana. RVE1 and RVE2 expression is downregulated after imBiBition and By phyB. RVE1 directly Binds to the promoter of GIBBERELLIN 3-OXIDASE 2, inhiBits its transcription and thus suppresses the Biosynthesis of Bioactive giBBerellins. In addition, DELAY OF GERMINATION 1 also acts downstream of phyB. This study identifies a signalling pathway that integrates environmental light input with internal factors to control Both seed dormancy and germination.
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<B>PhytochromeB> B and reveille1 2 mediated signalling controls seed dormancy and germination in araBidopsis
Nature Communications, 2016Co-Authors: Zhimin Jiang, Yanjun Jing, Weijiang Tang, Rongcheng LinAbstract:Seeds maintain a dormant state to withstand adverse conditions and germinate when conditions Become favouraBle to give rise to a new generation of flowering plants. Seed dormancy and germination are tightly controlled By internal and external signals. Although <B>PhytochromeB> photoreceptors are proposed to regulate primary seed dormancy, the underlying molecular mechanism remains elusive. Here we show that the REVEILLE1 (RVE1) and RVE2 transcription factors promote primary seed dormancy and repress red/far-red-light-reversiBle germination downstream of <B>PhytochromeB> B (phyB) in AraBidopsis thaliana. RVE1 and RVE2 expression is downregulated after imBiBition and By phyB. RVE1 directly Binds to the promoter of GIBBERELLIN 3-OXIDASE 2, inhiBits its transcription and thus suppresses the Biosynthesis of Bioactive giBBerellins. In addition, DELAY OF GERMINATION 1 also acts downstream of phyB. This study identifies a signalling pathway that integrates environmental light input with internal factors to control Both seed dormancy and germination. Seed dormancy prevents germination under adverse environmental conditions. Here the authors show that the RVE1 and RVE2 transcription factors suppress Biosynthesis of the germination-promoting hormone GA and act downstream of <B>PhytochromeB> signalling to promote dormancy and repress light-induced germination.
Rongcheng Lin - One of the best experts on this subject based on the ideXlab platform.
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fhy3 interacts with <B>PhytochromeB> B and regulates seed dormancy and germination
Plant Physiology, 2021Co-Authors: Shuangrong Liu, Weijiang Tang, Liwen Yang, Rongcheng LinAbstract:Seed dormancy and germination are fundamental processes for plant propagation, Both of which are tightly regulated By internal and external cues. <B>PhytochromeB> B (phyB) is a major red/far-red-aBsorBing photoreceptor that senses light signals that modulate seed dormancy and germination. However, the components that directly transduce that signal downstream of phyB are mostly unknown. Here, we show that the transposase-derived transcription factor FAR-RED ELONGATED HYPOCOTYL3 (FHY3) inhiBits seed dormancy and promotes phyB-mediated seed germination in AraBidopsis thaliana. FHY3 physically interacts with phyB in vitro and in vivo. RNA-sequencing and RT-qPCR analyses showed that FHY3 regulates multiple downstream genes, including REVEILLE2 (RVE2), RVE7, and SPATULA (SPT). Yeast one-hyBrid, electrophoresis moBility shift, and chromatin immunoprecipitation assays demonstrated that FHY3 directly Binds these genes via a conserved FBS cis-element in their promoters. Furthermore, RVE2, RVE7, and GIBBERELLIN 3-OXIDASE 2 (GA3ox2) genetically act downstream of FHY3. Strikingly, light and phyB promote FHY3 protein accumulation. Our study reveals a transcriptional cascade consisting of phyB-FHY3-RVE2/RVE7/SPT-GA3ox2 that relays environmental light signals and thereBy controls seed dormancy and germination.
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<B>PhytochromeB> B and reveille1 2 mediated signalling controls seed dormancy and germination in araBidopsis
Nature Communications, 2016Co-Authors: Zhimin Jiang, Yanjun Jing, Weijiang Tang, Rongcheng LinAbstract:Seeds maintain a dormant state to withstand adverse conditions and germinate when conditions Become favouraBle to give rise to a new generation of flowering plants. Seed dormancy and germination are tightly controlled By internal and external signals. Although <B>PhytochromeB> photoreceptors are proposed to regulate primary seed dormancy, the underlying molecular mechanism remains elusive. Here we show that the REVEILLE1 (RVE1) and RVE2 transcription factors promote primary seed dormancy and repress red/far-red-light-reversiBle germination downstream of <B>PhytochromeB> B (phyB) in AraBidopsis thaliana. RVE1 and RVE2 expression is downregulated after imBiBition and By phyB. RVE1 directly Binds to the promoter of GIBBERELLIN 3-OXIDASE 2, inhiBits its transcription and thus suppresses the Biosynthesis of Bioactive giBBerellins. In addition, DELAY OF GERMINATION 1 also acts downstream of phyB. This study identifies a signalling pathway that integrates environmental light input with internal factors to control Both seed dormancy and germination. Seed dormancy prevents germination under adverse environmental conditions. Here the authors show that the RVE1 and RVE2 transcription factors suppress Biosynthesis of the germination-promoting hormone GA and act downstream of <B>PhytochromeB> signalling to promote dormancy and repress light-induced germination.