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Adriana Zerlotti Mercadante - One of the best experts on this subject based on the ideXlab platform.
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effect of pasteurization on flavonoids and carotenoids in citrus sinensis l osbeck cv cara cara and bahia juices
Journal of Agricultural and Food Chemistry, 2017Co-Authors: Elisa Brasili, Adriana Zerlotti Mercadante, Daniela Fojo Seixas Chaves, Ana Augusta Odorissi Xavier, Neuza Mariko Aymoto Hassimotto, Franco Maria LajoloAbstract:Orange juice is considered an excellent dietary source of several bioactive compounds with beneficial properties for human health. Citrus sinensis Osbeck cv. ‘Cara Cara’ is a bud mutation originated from ‘Washington’ navel orange, also known as ‘Bahia’ navel orange. The ascorbic acid, flavonoid, and carotenoid contents in pasteurized and nonpasteurized Bahia and Cara Cara juices using two LC-MS/MS platforms were investigated. Higher ascorbic acid content was observed in Bahia compared to Cara Cara in both pasteurized and nonpasteurized juices. Total flavanones content as well as hesperidin levels were higher in Cara Cara with respect to Bahia pasteurized juice. Cara Cara was also characterized by a significantly higher and diversified carotenoid content compared to Bahia juice with a mixture of (Z)-isomers of lycopene, all-E-β-carotene, phytoene, and Phytofluene isomers accounting for the highest carotenoid proportion. The exceptionally high carotenoid content of Cara Cara may be particularly interesting ...
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Effect of Pasteurization on Flavonoids and Carotenoids in Citrus sinensis (L.) Osbeck cv. ‘Cara Cara’ and ‘Bahia’ Juices
2017Co-Authors: Elisa Brasili, Adriana Zerlotti Mercadante, Neuza Mariko Aymoto Hassimotto, Daniela Seixas F. Chaves, Ana Augusta O. Xavier, Franco Maria LajoloAbstract:Orange juice is considered an excellent dietary source of several bioactive compounds with beneficial properties for human health. Citrus sinensis Osbeck cv. ‘Cara Cara’ is a bud mutation originated from ‘Washington’ navel orange, also known as ‘Bahia’ navel orange. The ascorbic acid, flavonoid, and carotenoid contents in pasteurized and nonpasteurized Bahia and Cara Cara juices using two LC-MS/MS platforms were investigated. Higher ascorbic acid content was observed in Bahia compared to Cara Cara in both pasteurized and nonpasteurized juices. Total flavanones content as well as hesperidin levels were higher in Cara Cara with respect to Bahia pasteurized juice. Cara Cara was also characterized by a significantly higher and diversified carotenoid content compared to Bahia juice with a mixture of (Z)-isomers of lycopene, all-E-β-carotene, phytoene, and Phytofluene isomers accounting for the highest carotenoid proportion. The exceptionally high carotenoid content of Cara Cara may be particularly interesting for nutritional or functional studies of uncommon carotenes in a citrus food matrix
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composition of carotenoids from commercial products of caja spondias lutea
Journal of Food Composition and Analysis, 2001Co-Authors: P S Hamano, Adriana Zerlotti MercadanteAbstract:Abstract The carotenoid composition and vitamin A value of Brazilian commercial products of caja (Spondias lutea), frozen pulps and pasteurized juices, were determined by high-performance liquid chromatography (HPLC). Both products contained the following carotenoids: phytoene, Phytofluene, all- trans - β -carotene, 13- cis -β -carotene, 9- cis - β -carotene, α -carotene, β -cryptoxanthin (cis and trans), zeinoxanthin, and lutein. The major carotenoid was β -cryptoxanthin ranging from 5.54 to 8.19 μg/g, followed by lutein (from 3.52 to 6.16 μg/g) and zeinoxanthin from 3.52 to 3.85 μg/g.β -Cryptoxanthin was also the carotenoid which gave the greatest contribution to the vitamin A value.
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carotenoids from guava psidium guajava l isolation and structure elucidation
Journal of Agricultural and Food Chemistry, 1999Co-Authors: Adriana Zerlotti Mercadante, Andrea Steck, Hanspeter PfanderAbstract:Sixteen carotenoids were isolated from the flesh of Brazilian red guavas (Psidium guajava L.). Their structures were established by means of UV−visible, 400 and 500 MHz 1H NMR, 120 and 125 MHz 13C NMR, mass, and circular dichroism spectra. The carotenoids were identified as Phytofluene, (all-E)-, (9Z)-, (13Z)-, and (15Z)-β-carotene, (all-E)-γ-carotene, (all-E)-, (9Z)-, (13Z)-, and (15Z)-lycopene, (all-E,3R)-β-cryptoxanthin, (all-E,3R)-rubixanthin, (all-E,3S,5R,8S)-cryptoflavin, (all-E,3R,3‘R,6‘R)-lutein, (all-E,3S,5R,6R,3‘S,5‘R,8‘R)-, and (all-E,3S,5R,6R,3‘S,5‘R,8‘S)-neochrome. Thirteen of the carotenoids identified are reported as guava carotenoids for the first time. Keywords: Carotenoids; isolation; LC/MS; NMR; guava; Psidium guajava L.
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carotenoids from guava psidium guajava l isolation and structure elucidation
Journal of Agricultural and Food Chemistry, 1999Co-Authors: Adriana Zerlotti Mercadante, Andrea Steck, Hanspeter PfanderAbstract:Sixteen carotenoids were isolated from the flesh of Brazilian red guavas (Psidium guajava L.). Their structures were established by means of UV−visible, 400 and 500 MHz 1H NMR, 120 and 125 MHz 13C NMR, mass, and circular dichroism spectra. The carotenoids were identified as Phytofluene, (all-E)-, (9Z)-, (13Z)-, and (15Z)-β-carotene, (all-E)-γ-carotene, (all-E)-, (9Z)-, (13Z)-, and (15Z)-lycopene, (all-E,3R)-β-cryptoxanthin, (all-E,3R)-rubixanthin, (all-E,3S,5R,8S)-cryptoflavin, (all-E,3R,3‘R,6‘R)-lutein, (all-E,3S,5R,6R,3‘S,5‘R,8‘R)-, and (all-E,3S,5R,6R,3‘S,5‘R,8‘S)-neochrome. Thirteen of the carotenoids identified are reported as guava carotenoids for the first time. Keywords: Carotenoids; isolation; LC/MS; NMR; guava; Psidium guajava L.
Christof B Steingass - One of the best experts on this subject based on the ideXlab platform.
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hplc dad apci esi msn analysis of carotenoids and α tocopherol in costa rican acrocomia aculeata fruits of varying maturity stages
Food Research International, 2018Co-Authors: Roland Schex, Veronika M Lieb, Victor M Jimenez, Patricia Esquivel, Ralf M Schweiggert, Reinhold Carle, Christof B SteingassAbstract:Carotenoids and tocopherols were characterised in the meso- and exocarp of wild-growing Costa Rican Acrocomia aculeata fruits. Comprehensive profiling of these lipophilic micronutrients in fruits of three varying maturity stages was conducted for the first time. A method for the simultaneous extraction and quantitation of carotenoids and α-tocopherol was developed and validated. Detailed HPLC-DAD-APCI/ESI-MSn analyses enabled the identification of α-tocopherol and 25 carotenoids. The latter comprised antheraxanthin, β-carotene, lutein, luteoxanthin, neoxanthin, phytoene, Phytofluene, violaxanthin, zeaxanthin, and several (Z)-isomers of the aforementioned compounds. Quantitation by HPLC-DAD/FLD revealed total carotenoid concentrations of 872±178 and 3075±407μg/100g fresh weight in the meso- and exocarp of fully ripe fruits, respectively. In both fruit fractions, progressing maturation resulted in the accumulation of phytoene, Phytofluene, (all-E)-zeaxanthin, (all-E)-antheraxanthin, and (all-E)-violaxanthin. Carotenoid profiling was supported by multivariate data analysis. Carotenoid precursors and xanthophyll cycle pigments characterised Macauba fruits of full maturity.
Antonio J Melendezmartinez - One of the best experts on this subject based on the ideXlab platform.
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skin carotenoids in public health and nutricosmetics the emerging roles and applications of the uv radiation absorbing colourless carotenoids phytoene and Phytofluene
Nutrients, 2019Co-Authors: Antonio J Melendezmartinez, Carla M Stinco, Paula MapellibrahmAbstract:In this work, the importance of dietary carotenoids in skin health and appearance is comprehensively reviewed and discussed. References are made to their applications in health-promoting and nutricosmetic products and the important public health implications that can be derived. Attention is focused on the colourless UV radiation (UVR)-absorbing dietary carotenoids phytoene and Phytofluene, which are attracting increased interest in food science and technology, nutrition, health and cosmetics. These compounds are major dietary carotenoids, readily bioavailable, and have been shown to be involved in several health-promoting actions, as pinpointed in recent reviews. The growing evidence that these unique UVR-absorbing carotenoids with distinctive structures, properties (light absorption, susceptibility to oxidation, rigidity, tendency to aggregation, or even fluorescence, in the case of Phytofluene) and activities can be beneficial in these contexts is highlighted. Additionally, the recommendation that the levels of these carotenoids are considered in properly assessing skin carotenoid status is made.
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impact of thermal treatments on the bioaccessibility of phytoene and Phytofluene in relation to changes in the microstructure and size of orange juice particles
Journal of Functional Foods, 2018Co-Authors: Paula Mapellibrahm, Lorenzo Zacarias, Maria Jesus Rodrigo, Carla M Stinco, Antonio J MelendezmartinezAbstract:Abstract The interest in phytoene and Phytofluene is expanding. In this study their bioaccessibility from thermally treated orange juices was analysed. Other carotenoids were also considered for comparison. Fresh, pasteurized, and ultrafrozen juices thawed at room temperature (UF-RT), in microwave oven (UF-MW), and in fridge (UF-FG) were investigated. Colourless carotenoids suffered less degradation as a result of ultrafreezing than xanthophylls. The carotenoid with highest bioaccessibility in the fresh juice was phytoene (10%). Total carotenoid bioaccessibility followed the order: Pasteurized > UF-MW > UF-RT > UF-FG > Fresh. The cells degradation followed this same order while the particle size followed the opposite, i.e. the samples with the higher bioaccessibility were those with lower particle size. Thus, it was concluded that thermal treatments could enhance the release of carotenoids from the matrix due to the degradation of cells which could explain the higher bioaccessibility. The best source of bioaccessible colourless carotenoids was UF-MW.
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preliminary data on the safety of phytoene and Phytofluene rich products for human use including topical application
Journal of Toxicology, 2018Co-Authors: Fabien Havas, Antonio J Melendezmartinez, Shlomo Krispin, Liki Von OppenbezalelAbstract:The colorless carotenoids phytoene and Phytofluene are comparatively understudied compounds found in common foods (e.g., tomatoes) and in human plasma, internal tissues, and skin. Being naturally present in common foods, their intake at dietary levels is not expected to present a safety concern. However, since the interest in these compounds in the context of many applications is expanding, it is important to conduct studies aimed at assessing their safety. We present here results of in vitro cytotoxicity and genotoxicity studies, revealing no significant cytotoxic or genotoxic potential and of short- and long-term human in vivo skin compatibility studies with phytoene- and Phytofluene-rich tomato and Dunaliella salina alga extracts, showing a lack of irritancy or sensitization reactions. These results support the safe use of phytoene- and Phytofluene-rich products in human topical applications.
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bioaccessibility of phytoene and Phytofluene is superior to other carotenoids from selected fruit and vegetable juices
Food Chemistry, 2017Co-Authors: Paula Mapellibrahm, Antonio J Melendezmartinez, Joana Cortereal, Torsten BohnAbstract:Phytoene and Phytofluene are major abundant dietary carotenoids largely ignored in the context of agro-food and health. The bioaccessibility of phytoene and Phytofluene in tomato, carrot, blood orange (sanguinello cultivar), and apricot juices was analysed following simulated gastro-intestinal digestion with coffee cream as a lipid source, and compared with that of other main carotenoids from these matrices. The bioaccessibility of phytoene and Phytofluene, and also total carotenoid bioaccessibility, followed the order: sanguinello>apricot>tomato>carrot. Phytoene was consistently the carotenoid with the highest bioaccessibility, up to 97%, generally followed by Phytofluene. The higher bioaccessibility of these carotenoids could mainly be due to their marked difference in chemical structure and matrix distribution. For most juices, cis-isomers presented a higher bioaccessibility than their all-trans counterparts (P<0.05). The dietary source that provided highest amounts of potentially absorbable phytoene/Phytofluene was by far tomato juice (5mg/250mL juice).
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in vitro antioxidant capacity of tomato products relationships with their lycopene phytoene Phytofluene and alpha tocopherol contents evaluation of interactions and correlation with reflectance measurements
Lwt - Food Science and Technology, 2016Co-Authors: Carla M Stinco, Isabel M Vicario, Francisco J Heredia, Antonio J MelendezmartinezAbstract:Abstract Tomato products were analysed for their carotenoids and α-tocopherol contents to study the contribution of individual compounds to the antioxidant capacity and assess the existence of interactions. Besides, the applicability of reflectance measurements for the estimation of the Trolox Equivalent Antioxidant Capacity (TEAC) was explored. Statistically significant regression coefficients were found between TEAC values and carotenoids and α-tocopherol levels. Lycopene seemed to have the highest contribution, followed by Phytofluene. The results showed that the antioxidant capacity of Phytofluene warrants further investigation. It was observed that there can be interactions between lycopene and α-tocopherol, although just the lycopene/α-tocopherol ratio cannot be used to predict the kind of interaction. More knowledge of these interactions would supply new tools for the industry to develop more optimized ingredients. Finally, the lycopene levels and TEAC values can be estimated by considering reflectance readings at certain wavelengths, which is important for field and quality control applications.
Victor M Jimenez - One of the best experts on this subject based on the ideXlab platform.
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hplc dad apci esi msn analysis of carotenoids and α tocopherol in costa rican acrocomia aculeata fruits of varying maturity stages
Food Research International, 2018Co-Authors: Roland Schex, Veronika M Lieb, Victor M Jimenez, Patricia Esquivel, Ralf M Schweiggert, Reinhold Carle, Christof B SteingassAbstract:Carotenoids and tocopherols were characterised in the meso- and exocarp of wild-growing Costa Rican Acrocomia aculeata fruits. Comprehensive profiling of these lipophilic micronutrients in fruits of three varying maturity stages was conducted for the first time. A method for the simultaneous extraction and quantitation of carotenoids and α-tocopherol was developed and validated. Detailed HPLC-DAD-APCI/ESI-MSn analyses enabled the identification of α-tocopherol and 25 carotenoids. The latter comprised antheraxanthin, β-carotene, lutein, luteoxanthin, neoxanthin, phytoene, Phytofluene, violaxanthin, zeaxanthin, and several (Z)-isomers of the aforementioned compounds. Quantitation by HPLC-DAD/FLD revealed total carotenoid concentrations of 872±178 and 3075±407μg/100g fresh weight in the meso- and exocarp of fully ripe fruits, respectively. In both fruit fractions, progressing maturation resulted in the accumulation of phytoene, Phytofluene, (all-E)-zeaxanthin, (all-E)-antheraxanthin, and (all-E)-violaxanthin. Carotenoid profiling was supported by multivariate data analysis. Carotenoid precursors and xanthophyll cycle pigments characterised Macauba fruits of full maturity.
Reinhold Carle - One of the best experts on this subject based on the ideXlab platform.
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hplc dad apci esi msn analysis of carotenoids and α tocopherol in costa rican acrocomia aculeata fruits of varying maturity stages
Food Research International, 2018Co-Authors: Roland Schex, Veronika M Lieb, Victor M Jimenez, Patricia Esquivel, Ralf M Schweiggert, Reinhold Carle, Christof B SteingassAbstract:Carotenoids and tocopherols were characterised in the meso- and exocarp of wild-growing Costa Rican Acrocomia aculeata fruits. Comprehensive profiling of these lipophilic micronutrients in fruits of three varying maturity stages was conducted for the first time. A method for the simultaneous extraction and quantitation of carotenoids and α-tocopherol was developed and validated. Detailed HPLC-DAD-APCI/ESI-MSn analyses enabled the identification of α-tocopherol and 25 carotenoids. The latter comprised antheraxanthin, β-carotene, lutein, luteoxanthin, neoxanthin, phytoene, Phytofluene, violaxanthin, zeaxanthin, and several (Z)-isomers of the aforementioned compounds. Quantitation by HPLC-DAD/FLD revealed total carotenoid concentrations of 872±178 and 3075±407μg/100g fresh weight in the meso- and exocarp of fully ripe fruits, respectively. In both fruit fractions, progressing maturation resulted in the accumulation of phytoene, Phytofluene, (all-E)-zeaxanthin, (all-E)-antheraxanthin, and (all-E)-violaxanthin. Carotenoid profiling was supported by multivariate data analysis. Carotenoid precursors and xanthophyll cycle pigments characterised Macauba fruits of full maturity.