The Experts below are selected from a list of 705 Experts worldwide ranked by ideXlab platform
Qiang Xu - One of the best experts on this subject based on the ideXlab platform.
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anti inflammatory and immunosuppressive effect of flavones isolated from artemisia vestita
Journal of Ethnopharmacology, 2008Co-Authors: Fangyuan Gong, Yihua Li, Xingxin Wu, Ting Chen, Qiang XuAbstract:Abstract Aim of the study Artemisia vestita is a common traditional Tibetan medicinal plant which has been used widely in China for treating various inflammatory diseases. Since little is known about its active components, the purpose of this study was to isolate and identify the immunosuppressive compounds from Artemisia vestita . Materials and methods A bioassay-guided isolation was performed with Picryl Chloride-induced contact hypersensitivity in mice. MTT assay and Flow cytometric analysis were used for determining Con A-induced lymphocyte proliferation and CD25 expression in T cells, respectively. Results The ethanol extract of the Artemisia vestita was found to possess significant inhibitory activity against the Picryl Chloride-induced contact hypersensitivity in mice. Then 4 fractions were isolated by macroporous adsorption resin and one of these fractions (AV3), which showed the highest activity in in vivo test, was further subjected to column chromatography. Nine known flavones were isolated and identified as pectolinarigenin ( 1 ), jaceosidin ( 2 ), cirsilineol ( 3 ), cirsimaritin ( 4 ), hispidulin ( 5 ), quercetin ( 6 ), 6-methoxytricin ( 7 ), acacetin ( 8 ), and apigenin ( 9 ). The structures of the 9 flavones were elucidated by spectral techniques. All the compounds were evaluated for their inhibitory activity on the proliferation and activation of T cells in vitro . Among the 9 flavones, cirsilineol ( 3 ), 6-methoxytricin ( 7 ) and apigenin ( 9 ) significantly inhibited T cell proliferation and activation in the bioassays. Conclusion The result suggests that cirsilineol, 6-methoxytricin and apigenin are the major active components in Artemisia vestita .
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aqueous extract from aerial parts of artemisia vestita a traditional tibetan medicine reduces contact sensitivity in mice by down regulating the activation adhesion and metalloproteinase production of t lymphocytes
International Immunopharmacology, 2005Co-Authors: Jule Wang, Yihua Li, Qiang XuAbstract:In the present paper, the effect of the aqueous extract from aerial parts of Artemisia vestita (AV-ext), a traditional Tibetan medicine, on ear contact sensitivity was examined. AV-ext significantly reduced the ear swelling when administered during the induction phase of Picryl-Chloride (PCl)-induced ear contact sensitivity in mice. The extract also showed a dose-dependent inhibition on lymphocyte proliferation and IL-2 production in Con A-activated spleen cells. The proliferation inhibition was confirmed in the mixed lymphocytes reaction. Furthermore, the adhesion of the isolated spleen cells from PCl-sensitized mice to type IV collagen was significantly decreased in a dose-dependent manner by AV-ext. Such decrease was also seen in AV-ext-treated Jurkat T cells and the T cells purified from above spleen cells. The purified spleen T cells from PCl-sensitized mice produced more matrix metalloproteinase-9 (MMP-9) than naive T cells, and AV-ext remarkably reduced MMP-9 production both in vivo and in vitro. These results suggest that AV-ext may alleviate contact sensitivity through blocking the activation of T lymphocytes and decreasing their localization to the inflammatory sites via down-regulating the potential of cell adhesion and metalloproteinase production.
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ruscogenin glycoside lm 3 isolated from liriope muscari improves liver injury by dysfunctioning liver infiltrating lymphocytes
Journal of Pharmacy and Pharmacology, 2001Co-Authors: Feihua Wu, Qiang Xu, Jieyun Jiang, Boyang YuAbstract:The effects of ruscogenin 1-O-[β-d-glucopyranosyl(1 2)] [β-d-xylopyranosyl(1 3)]-β-d-fucopyranoside (Lm-3) and its aglycone, ruscogenin, on liver injury induced in mice by delayed-type hypersensitivity to Picryl Chloride have been investigated. Lm-3 and ruscogenin significantly decreased liver injury when given during the effector phase of the delayed-type hypersensitivity reaction. The pretreatment of nonparenchymal cells, but not hepatocytes, with Lm-3 or ruscogenin in-vitro caused a concentration- and time-dependent inhibition against the damage. Lm-3 showed a stronger inhibition against the damage than ruscogenin (IC50: Lm-3 6.3 times 10−10m, ruscogenin 3.9 times 10−7m). However, neither Lm-3 nor ruscogenin blocked the hepatotoxic potential of CCl4, when used to pretreat hepatocytes. Moreover, Lm-3 and ruscogenin inhibited concanavalin A-induced lymphocyte proliferation only at high concentrations. These results suggested that Lm-3 and ruscogenin improved the immunological liver injury by selectively causing dysfunction of the liver-infiltrating cells rather than by protecting hepatocyte membranes. Such characteristics would be significant for treating immunologically related liver diseases as well as for developing new drugs.
Jieyun Jiang - One of the best experts on this subject based on the ideXlab platform.
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ruscogenin glycoside lm 3 isolated from liriope muscari improves liver injury by dysfunctioning liver infiltrating lymphocytes
Journal of Pharmacy and Pharmacology, 2010Co-Authors: Jingsong Cao, Jieyun JiangAbstract:The effects of ruscogenin 1-O-[beta-D-glucopyranosyl(1 --> 2)] [beta-D-xylopyranosyl(1 --> 3)]-beta-D-fucopyranoside (Lm-3) and its aglycone, ruscogenin, on liver injury induced in mice by delayed-type hypersensitivity to Picryl Chloride have been investigated. Lm-3 and ruscogenin significantly decreased liver injury when given during the effector phase of the delayed-type hypersensitivity reaction. The pretreatment of nonparenchymal cells, but not hepatocytes, with Lm-3 or ruscogenin in-vitro caused a concentration- and time-dependent inhibition against the damage. Lm-3 showed a stronger inhibition against the damage than ruscogenin (IC50: Lm-3 6.3 x 10(-10) M, ruscogenin 3.9 x 10(-7) M). However, neither Lm-3 nor ruscogenin blocked the hepatotoxic potential of CCl4, when used to pretreat hepatocytes. Moreover, Lm-3 and ruscogenin inhibited concanavalin A-induced lymphocyte proliferation only at high concentrations. These results suggested that Lm-3 and ruscogenin improved the immunological liver injury by selectively causing dysfunction of the liver-infiltrating cells rather than by protecting hepatocyte membranes. Such characteristics would be significant for treating immunologically related liver diseases as well as for developing new drugs.
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immunomodulatory activity of the aqueous extract from rhizome of smilax glabra in the later phase of adjuvant induced arthritis in rats
Journal of Ethnopharmacology, 2003Co-Authors: Jieyun JiangAbstract:Our previous paper has reported that the aqueous extract from Rhizoma Smilacis Glabrae (RSG) remarkably inhibited the primary inflammation of adjuvant arthritis (AA) in rats. In the present study, we further examined the activity of RSG and its mechanism on the secondary inflammation of AA. The administration of RSG (400 and 800 mg/kg) during the later phase significantly inhibited the swelling of the adjuvant-non-injected footpad of AA rats. The lipopolysaccharide-induced production of IL-1, TNF and NO by peritoneal macrophages was significantly reduced. In contrast, the extract significantly recovered the decrease in weight gain of the AA rats and Concanavalin A-induced T lymphocyte proliferation and IL-2 production by their splenocytes, while prednisolone (10 mg/kg) showed a significant aggravation. Furthermore, RSG significantly recovered the Picryl Chloride-induced delayed-type hypersensitivity to almost normal levels from the higher or lower levels induced by different treatments of cyclophosphamide with a normalization of CD4/CD8 ratio. These results suggest that RSG exhibit an improvement on AA through down-regulating over-activated macrophages and up-regulating the dysfunctional T lymphocytes during the later phase of arthritis. Such characteristics of RSG on AA may be advantageous to the long-term treatment of clinical rheumatoid arthritis.
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ruscogenin glycoside lm 3 isolated from liriope muscari improves liver injury by dysfunctioning liver infiltrating lymphocytes
Journal of Pharmacy and Pharmacology, 2001Co-Authors: Feihua Wu, Qiang Xu, Jieyun Jiang, Boyang YuAbstract:The effects of ruscogenin 1-O-[β-d-glucopyranosyl(1 2)] [β-d-xylopyranosyl(1 3)]-β-d-fucopyranoside (Lm-3) and its aglycone, ruscogenin, on liver injury induced in mice by delayed-type hypersensitivity to Picryl Chloride have been investigated. Lm-3 and ruscogenin significantly decreased liver injury when given during the effector phase of the delayed-type hypersensitivity reaction. The pretreatment of nonparenchymal cells, but not hepatocytes, with Lm-3 or ruscogenin in-vitro caused a concentration- and time-dependent inhibition against the damage. Lm-3 showed a stronger inhibition against the damage than ruscogenin (IC50: Lm-3 6.3 times 10−10m, ruscogenin 3.9 times 10−7m). However, neither Lm-3 nor ruscogenin blocked the hepatotoxic potential of CCl4, when used to pretreat hepatocytes. Moreover, Lm-3 and ruscogenin inhibited concanavalin A-induced lymphocyte proliferation only at high concentrations. These results suggested that Lm-3 and ruscogenin improved the immunological liver injury by selectively causing dysfunction of the liver-infiltrating cells rather than by protecting hepatocyte membranes. Such characteristics would be significant for treating immunologically related liver diseases as well as for developing new drugs.
Feihua Wu - One of the best experts on this subject based on the ideXlab platform.
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ruscogenin glycoside lm 3 isolated from liriope muscari improves liver injury by dysfunctioning liver infiltrating lymphocytes
Journal of Pharmacy and Pharmacology, 2001Co-Authors: Feihua Wu, Qiang Xu, Jieyun Jiang, Boyang YuAbstract:The effects of ruscogenin 1-O-[β-d-glucopyranosyl(1 2)] [β-d-xylopyranosyl(1 3)]-β-d-fucopyranoside (Lm-3) and its aglycone, ruscogenin, on liver injury induced in mice by delayed-type hypersensitivity to Picryl Chloride have been investigated. Lm-3 and ruscogenin significantly decreased liver injury when given during the effector phase of the delayed-type hypersensitivity reaction. The pretreatment of nonparenchymal cells, but not hepatocytes, with Lm-3 or ruscogenin in-vitro caused a concentration- and time-dependent inhibition against the damage. Lm-3 showed a stronger inhibition against the damage than ruscogenin (IC50: Lm-3 6.3 times 10−10m, ruscogenin 3.9 times 10−7m). However, neither Lm-3 nor ruscogenin blocked the hepatotoxic potential of CCl4, when used to pretreat hepatocytes. Moreover, Lm-3 and ruscogenin inhibited concanavalin A-induced lymphocyte proliferation only at high concentrations. These results suggested that Lm-3 and ruscogenin improved the immunological liver injury by selectively causing dysfunction of the liver-infiltrating cells rather than by protecting hepatocyte membranes. Such characteristics would be significant for treating immunologically related liver diseases as well as for developing new drugs.
Jingsong Cao - One of the best experts on this subject based on the ideXlab platform.
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ruscogenin glycoside lm 3 isolated from liriope muscari improves liver injury by dysfunctioning liver infiltrating lymphocytes
Journal of Pharmacy and Pharmacology, 2010Co-Authors: Jingsong Cao, Jieyun JiangAbstract:The effects of ruscogenin 1-O-[beta-D-glucopyranosyl(1 --> 2)] [beta-D-xylopyranosyl(1 --> 3)]-beta-D-fucopyranoside (Lm-3) and its aglycone, ruscogenin, on liver injury induced in mice by delayed-type hypersensitivity to Picryl Chloride have been investigated. Lm-3 and ruscogenin significantly decreased liver injury when given during the effector phase of the delayed-type hypersensitivity reaction. The pretreatment of nonparenchymal cells, but not hepatocytes, with Lm-3 or ruscogenin in-vitro caused a concentration- and time-dependent inhibition against the damage. Lm-3 showed a stronger inhibition against the damage than ruscogenin (IC50: Lm-3 6.3 x 10(-10) M, ruscogenin 3.9 x 10(-7) M). However, neither Lm-3 nor ruscogenin blocked the hepatotoxic potential of CCl4, when used to pretreat hepatocytes. Moreover, Lm-3 and ruscogenin inhibited concanavalin A-induced lymphocyte proliferation only at high concentrations. These results suggested that Lm-3 and ruscogenin improved the immunological liver injury by selectively causing dysfunction of the liver-infiltrating cells rather than by protecting hepatocyte membranes. Such characteristics would be significant for treating immunologically related liver diseases as well as for developing new drugs.
Ting Chen - One of the best experts on this subject based on the ideXlab platform.
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anti inflammatory and immunosuppressive effect of flavones isolated from artemisia vestita
Journal of Ethnopharmacology, 2008Co-Authors: Fangyuan Gong, Yihua Li, Xingxin Wu, Ting Chen, Qiang XuAbstract:Abstract Aim of the study Artemisia vestita is a common traditional Tibetan medicinal plant which has been used widely in China for treating various inflammatory diseases. Since little is known about its active components, the purpose of this study was to isolate and identify the immunosuppressive compounds from Artemisia vestita . Materials and methods A bioassay-guided isolation was performed with Picryl Chloride-induced contact hypersensitivity in mice. MTT assay and Flow cytometric analysis were used for determining Con A-induced lymphocyte proliferation and CD25 expression in T cells, respectively. Results The ethanol extract of the Artemisia vestita was found to possess significant inhibitory activity against the Picryl Chloride-induced contact hypersensitivity in mice. Then 4 fractions were isolated by macroporous adsorption resin and one of these fractions (AV3), which showed the highest activity in in vivo test, was further subjected to column chromatography. Nine known flavones were isolated and identified as pectolinarigenin ( 1 ), jaceosidin ( 2 ), cirsilineol ( 3 ), cirsimaritin ( 4 ), hispidulin ( 5 ), quercetin ( 6 ), 6-methoxytricin ( 7 ), acacetin ( 8 ), and apigenin ( 9 ). The structures of the 9 flavones were elucidated by spectral techniques. All the compounds were evaluated for their inhibitory activity on the proliferation and activation of T cells in vitro . Among the 9 flavones, cirsilineol ( 3 ), 6-methoxytricin ( 7 ) and apigenin ( 9 ) significantly inhibited T cell proliferation and activation in the bioassays. Conclusion The result suggests that cirsilineol, 6-methoxytricin and apigenin are the major active components in Artemisia vestita .
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role of four major components in the effect of si ni san a traditional chinese prescription against contact sensitivity in mice
Journal of Pharmacy and Pharmacology, 2006Co-Authors: Li Zhang, Yang Sun, Yi Dong, Ting ChenAbstract:Previously, we demonstrated the inhibitory effects of Si-Ni-San, a traditional Chinese prescription, on Picryl Chloride-induced ear contact sensitivity (PCl-CS). This study aimed to evaluate the role of the four major constituents contained in the prescription (saikosaponins, paeoniflorin, naringin and glycyrrhizin) in the inhibitory effect. When administered during the induction phase, saikosaponin a and glycyrrhizin showed significant inhibitory effects, while paeoniflorin and naringin did not. These components in Si-Ni-San also inhibited the activation and proliferation of T lymphocytes as well as the production of cytokines such as tumour necrosis factor-alpha and interferon-gamma to different extents. Saikosaponin a and paeoniflorin dose-dependently reduced the splenocyte adhesion to type I collagen, while glycyrrhizin only showed a slight tendency. Furthermore, treatment with glycyrrhizin or saikosaponin a, rather than paeoniflorin or naringin, moderately inhibited the matrix metalloproteinase (MMP)-2 activity of the splenocytes from PCl-CS mice, and the combination of all four components showed a strong inhibition against MMP-2. Moreover, the components markedly decreased the serum level of nitric oxide in PCl-sensitized mice. The results indicated that saikosaponin a and glycyrrhizin may be the major contributors in the alleviation effect of Si-Ni-San on contact sensitivity, and paeoniflorin and naringin may exhibit a co-operative effect.
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astilbin suppresses delayed type hypersensitivity by inhibiting lymphocyte migration
Journal of Pharmacy and Pharmacology, 2003Co-Authors: Yu Cai, Ting ChenAbstract:This study examined the effects of astilbin, a flavanone, on delayed-type hypersensitivity reactions and its mechanisms of action on cell migration. Astilbin significantly inhibited the sheep-red-blood-cell-induced footpad reaction and Picryl-Chloride-induced ear dermatitis without affecting the organ weights, when administered during the effector phase but not the induction phase. The flavanone also significantly inhibited the migration to gelatin of spleen cells isolated from mice with ear dermatitis in a transwell system. Furthermore, the isolated spleen cells from normal mice were incubated with astilbin in the presence of concanavalin A, or those from mice with ear dermatitis were cultured with astilbin. In the supernatants collected, the activity of matrix metalloproteinases (MMPs) MMP-2 and MMP-9 was remarkably inhibited by astilbin. These results suggest that astilbin may inhibit delayed-type hypersensitivity reactions through selectively suppressing the lymphocyte functions, including cell migration, via down-regulating MMP activity.