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Gerald T. Ankley - One of the best experts on this subject based on the ideXlab platform.
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first generation annotations for the fathead minnow Pimephales Promelas genome
Environmental Toxicology and Chemistry, 2017Co-Authors: Travis W Saari, Gerald T. Ankley, Anthony L Schroeder, Daniel L VilleneuveAbstract:Ab initio gene prediction and evidence alignment were used to produce the first annotations for the fathead minnow (Pimephales Promelas) genome. We also describe a genome browser, hosted by the Society of Environmental Toxicology and Chemistry, that provides simplified access to the annotation data in context with the genomic sequence. The present study extends the utility of the fathead minnow genome and supports the continued development of this species as a model organism for predictive toxicology. Environ Toxicol Chem 2017;9999:1–7. Published 2017 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.
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sequencing and de novo draft assemblies of a fathead minnow Pimephales Promelas reference genome
Environmental Toxicology and Chemistry, 2016Co-Authors: Frank R Burns, Daniel L Villeneuve, Gerald T. Ankley, Amarin L Cogburn, Eric R Waits, Yunjuan Chang, Victor Llaca, Stephane Deschamps, Raymond E Jackson, Robert A HokeAbstract:The present study was undertaken to provide the foundation for development of genome-scale resources for the fathead minnow (Pimephales Promelas), an important model organism widely used in both aquatic toxicology research and regulatory testing. The authors report on the first sequencing and 2 draft assemblies for the reference genome of this species. Approximately 120× sequence coverage was achieved via Illumina sequencing of a combination of paired-end, mate-pair, and fosmid libraries. Evaluation and comparison of these assemblies demonstrate that they are of sufficient quality to be useful for genome-enabled studies, with 418 of 458 (91%) conserved eukaryotic genes mapping to at least 1 of the assemblies. In addition to its immediate utility, the present work provides a strong foundation on which to build further refinements of a reference genome for the fathead minnow. Environ Toxicol Chem 2016;35:212–217. © 2015 SETAC
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sequencing and de novo draft assemblies of a fathead minnow Pimephales Promelas reference genome
Environmental Toxicology and Chemistry, 2016Co-Authors: Frank R Burns, Daniel L Villeneuve, Gerald T. Ankley, Amarin L Cogburn, Eric R Waits, Yunjuan Chang, Victor Llaca, Stephane Deschamps, Raymond E Jackson, Robert A HokeAbstract:The present study was undertaken to provide the foundation for development of genome-scale resources for the fathead minnow (Pimephales Promelas), an important model organism widely used in both aquatic toxicology research and regulatory testing. The authors report on the first sequencing and 2 draft assemblies for the reference genome of this species. Approximately 120× sequence coverage was achieved via Illumina sequencing of a combination of paired-end, mate-pair, and fosmid libraries. Evaluation and comparison of these assemblies demonstrate that they are of sufficient quality to be useful for genome-enabled studies, with 418 of 458 (91%) conserved eukaryotic genes mapping to at least 1 of the assemblies. In addition to its immediate utility, the present work provides a strong foundation on which to build further refinements of a reference genome for the fathead minnow.
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effects of gemfibrozil on lipid metabolism steroidogenesis and reproduction in the fathead minnow Pimephales Promelas
Environmental Toxicology and Chemistry, 2012Co-Authors: Sarah Y Skolness, Daniel L Villeneuve, Kathleen M Jensen, Michael D Kahl, Elizabeth A Makynen, Elizabeth J Durhan, Gerald T. AnkleyAbstract:Fibrates are a class of pharmaceuticals that indirectly modulate cholesterol biosynthesis through effects on peroxisome proliferator-activated receptors. Gemfibrozil is a fibrate that has been detected in wastewater treatment plant influents, effluents, and drinking water. The objective of the present study was to assess the potential physiological and reproductive impacts of gemfibrozil on fathead minnows (Pimephales Promelas). Fish were exposed to gemfibrozil in two different studies. The first was a short-term test with water concentrations of 0, 15, and 600 µg gemfibrozil/L, sampling after 2 or 8 d of exposure. Plasma cholesterol concentrations were significantly reduced in males exposed to 600 µg gemfibrozil/L for 8 d. In addition, expression of several hepatic genes important to lipid metabolism was altered, suggesting that gemfibrozil does affect lipid metabolism in fish. A 21-d study was conducted to investigate further the effects on lipid metabolism and steroidogenesis as well as to assess potential impacts of gemfibrozil on reproduction. Fish were exposed to water concentrations of 0, 1.5, 15, 600, and 1,500 µg gemfibrozil/L. Exposure to 1,500 µg gemfibrozil/L caused a modest, but not significant, reduction in fecundity. However, gemfibrozil had no consistent effect on plasma cholesterol, triglycerides, or sex steroids after 21 d of exposure. The present study showed no evidence for significant physiological or reproductive impacts of gemfibrozil at an environmentally relevant concentration of 1.5 µg/L.
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effects of gemfibrozil on lipid metabolism steroidogenesis and reproduction in the fathead minnow Pimephales Promelas
Environmental Toxicology and Chemistry, 2012Co-Authors: Sarah Y Skolness, Daniel L Villeneuve, Kathleen M Jensen, Michael D Kahl, Elizabeth A Makynen, Elizabeth J Durhan, Gerald T. AnkleyAbstract:Fibrates are a class of pharmaceuticals that indirectly modulate cholesterol biosynthesis through effects on peroxisome proliferator-activated receptors. Gemfibrozil is a fibrate that has been detected in wastewater treatment plant influents, effluents, and drinking water. The objective of the present study was to assess the potential physiological and reproductive impacts of gemfibrozil on fathead minnows (Pimephales Promelas). Fish were exposed to gemfibrozil in two different studies. The first was a short-term test with water concentrations of 0, 15, and 600 µg gemfibrozil/L, sampling after 2 or 8 d of exposure. Plasma cholesterol concentrations were significantly reduced in males exposed to 600 µg gemfibrozil/L for 8 d. In addition, expression of several hepatic genes important to lipid metabolism was altered, suggesting that gemfibrozil does affect lipid metabolism in fish. A 21-d study was conducted to investigate further the effects on lipid metabolism and steroidogenesis as well as to assess potential impacts of gemfibrozil on reproduction. Fish were exposed to water concentrations of 0, 1.5, 15, 600, and 1,500 µg gemfibrozil/L. Exposure to 1,500 µg gemfibrozil/L caused a modest, but not significant, reduction in fecundity. However, gemfibrozil had no consistent effect on plasma cholesterol, triglycerides, or sex steroids after 21 d of exposure. The present study showed no evidence for significant physiological or reproductive impacts of gemfibrozil at an environmentally relevant concentration of 1.5 µg/L. Environ. Toxicol. Chem. 2012; 31: 2615–2624. © 2012 SETAC
Daniel L Villeneuve - One of the best experts on this subject based on the ideXlab platform.
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first generation annotations for the fathead minnow Pimephales Promelas genome
Environmental Toxicology and Chemistry, 2017Co-Authors: Travis W Saari, Gerald T. Ankley, Anthony L Schroeder, Daniel L VilleneuveAbstract:Ab initio gene prediction and evidence alignment were used to produce the first annotations for the fathead minnow (Pimephales Promelas) genome. We also describe a genome browser, hosted by the Society of Environmental Toxicology and Chemistry, that provides simplified access to the annotation data in context with the genomic sequence. The present study extends the utility of the fathead minnow genome and supports the continued development of this species as a model organism for predictive toxicology. Environ Toxicol Chem 2017;9999:1–7. Published 2017 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.
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sequencing and de novo draft assemblies of a fathead minnow Pimephales Promelas reference genome
Environmental Toxicology and Chemistry, 2016Co-Authors: Frank R Burns, Daniel L Villeneuve, Gerald T. Ankley, Amarin L Cogburn, Eric R Waits, Yunjuan Chang, Victor Llaca, Stephane Deschamps, Raymond E Jackson, Robert A HokeAbstract:The present study was undertaken to provide the foundation for development of genome-scale resources for the fathead minnow (Pimephales Promelas), an important model organism widely used in both aquatic toxicology research and regulatory testing. The authors report on the first sequencing and 2 draft assemblies for the reference genome of this species. Approximately 120× sequence coverage was achieved via Illumina sequencing of a combination of paired-end, mate-pair, and fosmid libraries. Evaluation and comparison of these assemblies demonstrate that they are of sufficient quality to be useful for genome-enabled studies, with 418 of 458 (91%) conserved eukaryotic genes mapping to at least 1 of the assemblies. In addition to its immediate utility, the present work provides a strong foundation on which to build further refinements of a reference genome for the fathead minnow.
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sequencing and de novo draft assemblies of a fathead minnow Pimephales Promelas reference genome
Environmental Toxicology and Chemistry, 2016Co-Authors: Frank R Burns, Daniel L Villeneuve, Gerald T. Ankley, Amarin L Cogburn, Eric R Waits, Yunjuan Chang, Victor Llaca, Stephane Deschamps, Raymond E Jackson, Robert A HokeAbstract:The present study was undertaken to provide the foundation for development of genome-scale resources for the fathead minnow (Pimephales Promelas), an important model organism widely used in both aquatic toxicology research and regulatory testing. The authors report on the first sequencing and 2 draft assemblies for the reference genome of this species. Approximately 120× sequence coverage was achieved via Illumina sequencing of a combination of paired-end, mate-pair, and fosmid libraries. Evaluation and comparison of these assemblies demonstrate that they are of sufficient quality to be useful for genome-enabled studies, with 418 of 458 (91%) conserved eukaryotic genes mapping to at least 1 of the assemblies. In addition to its immediate utility, the present work provides a strong foundation on which to build further refinements of a reference genome for the fathead minnow. Environ Toxicol Chem 2016;35:212–217. © 2015 SETAC
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propiconazole inhibits steroidogenesis and reproduction in the fathead minnow Pimephales Promelas
Chemical Hazards in Industry, 2013Co-Authors: Sarah Y Skolness, Rodney D Johnson, Kathleen M Jensen, Michael D Kahl, Elizabeth A Makynen, Elizabeth J Durhan, Jenna E. Cavallin, Chad Blanksma, Jessica J Churchill, Daniel L VilleneuveAbstract:Conazoles are designed to inhibit cytochrome P450 (CYP) 14α-demethylase, an enzyme key to fungal cell wall formation. In vertebrates, conazoles may inhibit other CYPs, potentially disrupting processes like sex steroid synthesis. Propiconazole is a current-use pesticide that is among the first chemicals being tested in the U.S. Environmental Protection Agency endocrine disruptor screening program. Fathead minnows (Pimephales Promelas) were exposed to 0, 5, 50, 500, or 1000 µg propiconazole/l in a 21-day study that evaluated apical reproductive endpoints (fecundity, fertility, hatch); measures of endocrine function and steroid synthesis, such as cholesterol, vitellogenin (VTG), and sex steroid (testosterone (T), 17β-estradiol (E2)) concentrations in the plasma; and changes in gonadal expression of steroidogenic genes. Plasma E2 and VTG concentrations in females were reduced by exposure to propiconazole, and egg production was decreased in the 500 and 1000 µg/l treatment groups. These in vivo effects coincided with inhibition of E2 synthesis by ovary explants exposed to propiconazole in vitro. The authors also observed a compensatory response in females exposed to propiconazole, manifested as increased gonad weight and upregulation of genes coding for key steriodogenic proteins, including CYP19 (aromatase), CYP17 (hydroxylase/lyase), CYP11A (cholesterol side-chain-cleavage), and steroidogenic acute regulatory protein. Other than an increase in relative testis weight, effects on endocrine function in males were less pronounced than in females. This study provides important data relative to the potential endocrine activity of propiconazole in fish and, more generally, to the further delineation of pathways for the reproductive effects of steroid synthesis inhibitors in fish.
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effects of gemfibrozil on lipid metabolism steroidogenesis and reproduction in the fathead minnow Pimephales Promelas
Environmental Toxicology and Chemistry, 2012Co-Authors: Sarah Y Skolness, Daniel L Villeneuve, Kathleen M Jensen, Michael D Kahl, Elizabeth A Makynen, Elizabeth J Durhan, Gerald T. AnkleyAbstract:Fibrates are a class of pharmaceuticals that indirectly modulate cholesterol biosynthesis through effects on peroxisome proliferator-activated receptors. Gemfibrozil is a fibrate that has been detected in wastewater treatment plant influents, effluents, and drinking water. The objective of the present study was to assess the potential physiological and reproductive impacts of gemfibrozil on fathead minnows (Pimephales Promelas). Fish were exposed to gemfibrozil in two different studies. The first was a short-term test with water concentrations of 0, 15, and 600 µg gemfibrozil/L, sampling after 2 or 8 d of exposure. Plasma cholesterol concentrations were significantly reduced in males exposed to 600 µg gemfibrozil/L for 8 d. In addition, expression of several hepatic genes important to lipid metabolism was altered, suggesting that gemfibrozil does affect lipid metabolism in fish. A 21-d study was conducted to investigate further the effects on lipid metabolism and steroidogenesis as well as to assess potential impacts of gemfibrozil on reproduction. Fish were exposed to water concentrations of 0, 1.5, 15, 600, and 1,500 µg gemfibrozil/L. Exposure to 1,500 µg gemfibrozil/L caused a modest, but not significant, reduction in fecundity. However, gemfibrozil had no consistent effect on plasma cholesterol, triglycerides, or sex steroids after 21 d of exposure. The present study showed no evidence for significant physiological or reproductive impacts of gemfibrozil at an environmentally relevant concentration of 1.5 µg/L. Environ. Toxicol. Chem. 2012; 31: 2615–2624. © 2012 SETAC
Kathleen M Jensen - One of the best experts on this subject based on the ideXlab platform.
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propiconazole inhibits steroidogenesis and reproduction in the fathead minnow Pimephales Promelas
Chemical Hazards in Industry, 2013Co-Authors: Sarah Y Skolness, Rodney D Johnson, Kathleen M Jensen, Michael D Kahl, Elizabeth A Makynen, Elizabeth J Durhan, Jenna E. Cavallin, Chad Blanksma, Jessica J Churchill, Daniel L VilleneuveAbstract:Conazoles are designed to inhibit cytochrome P450 (CYP) 14α-demethylase, an enzyme key to fungal cell wall formation. In vertebrates, conazoles may inhibit other CYPs, potentially disrupting processes like sex steroid synthesis. Propiconazole is a current-use pesticide that is among the first chemicals being tested in the U.S. Environmental Protection Agency endocrine disruptor screening program. Fathead minnows (Pimephales Promelas) were exposed to 0, 5, 50, 500, or 1000 µg propiconazole/l in a 21-day study that evaluated apical reproductive endpoints (fecundity, fertility, hatch); measures of endocrine function and steroid synthesis, such as cholesterol, vitellogenin (VTG), and sex steroid (testosterone (T), 17β-estradiol (E2)) concentrations in the plasma; and changes in gonadal expression of steroidogenic genes. Plasma E2 and VTG concentrations in females were reduced by exposure to propiconazole, and egg production was decreased in the 500 and 1000 µg/l treatment groups. These in vivo effects coincided with inhibition of E2 synthesis by ovary explants exposed to propiconazole in vitro. The authors also observed a compensatory response in females exposed to propiconazole, manifested as increased gonad weight and upregulation of genes coding for key steriodogenic proteins, including CYP19 (aromatase), CYP17 (hydroxylase/lyase), CYP11A (cholesterol side-chain-cleavage), and steroidogenic acute regulatory protein. Other than an increase in relative testis weight, effects on endocrine function in males were less pronounced than in females. This study provides important data relative to the potential endocrine activity of propiconazole in fish and, more generally, to the further delineation of pathways for the reproductive effects of steroid synthesis inhibitors in fish.
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effects of gemfibrozil on lipid metabolism steroidogenesis and reproduction in the fathead minnow Pimephales Promelas
Environmental Toxicology and Chemistry, 2012Co-Authors: Sarah Y Skolness, Daniel L Villeneuve, Kathleen M Jensen, Michael D Kahl, Elizabeth A Makynen, Elizabeth J Durhan, Gerald T. AnkleyAbstract:Fibrates are a class of pharmaceuticals that indirectly modulate cholesterol biosynthesis through effects on peroxisome proliferator-activated receptors. Gemfibrozil is a fibrate that has been detected in wastewater treatment plant influents, effluents, and drinking water. The objective of the present study was to assess the potential physiological and reproductive impacts of gemfibrozil on fathead minnows (Pimephales Promelas). Fish were exposed to gemfibrozil in two different studies. The first was a short-term test with water concentrations of 0, 15, and 600 µg gemfibrozil/L, sampling after 2 or 8 d of exposure. Plasma cholesterol concentrations were significantly reduced in males exposed to 600 µg gemfibrozil/L for 8 d. In addition, expression of several hepatic genes important to lipid metabolism was altered, suggesting that gemfibrozil does affect lipid metabolism in fish. A 21-d study was conducted to investigate further the effects on lipid metabolism and steroidogenesis as well as to assess potential impacts of gemfibrozil on reproduction. Fish were exposed to water concentrations of 0, 1.5, 15, 600, and 1,500 µg gemfibrozil/L. Exposure to 1,500 µg gemfibrozil/L caused a modest, but not significant, reduction in fecundity. However, gemfibrozil had no consistent effect on plasma cholesterol, triglycerides, or sex steroids after 21 d of exposure. The present study showed no evidence for significant physiological or reproductive impacts of gemfibrozil at an environmentally relevant concentration of 1.5 µg/L. Environ. Toxicol. Chem. 2012; 31: 2615–2624. © 2012 SETAC
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effects of gemfibrozil on lipid metabolism steroidogenesis and reproduction in the fathead minnow Pimephales Promelas
Environmental Toxicology and Chemistry, 2012Co-Authors: Sarah Y Skolness, Daniel L Villeneuve, Kathleen M Jensen, Michael D Kahl, Elizabeth A Makynen, Elizabeth J Durhan, Gerald T. AnkleyAbstract:Fibrates are a class of pharmaceuticals that indirectly modulate cholesterol biosynthesis through effects on peroxisome proliferator-activated receptors. Gemfibrozil is a fibrate that has been detected in wastewater treatment plant influents, effluents, and drinking water. The objective of the present study was to assess the potential physiological and reproductive impacts of gemfibrozil on fathead minnows (Pimephales Promelas). Fish were exposed to gemfibrozil in two different studies. The first was a short-term test with water concentrations of 0, 15, and 600 µg gemfibrozil/L, sampling after 2 or 8 d of exposure. Plasma cholesterol concentrations were significantly reduced in males exposed to 600 µg gemfibrozil/L for 8 d. In addition, expression of several hepatic genes important to lipid metabolism was altered, suggesting that gemfibrozil does affect lipid metabolism in fish. A 21-d study was conducted to investigate further the effects on lipid metabolism and steroidogenesis as well as to assess potential impacts of gemfibrozil on reproduction. Fish were exposed to water concentrations of 0, 1.5, 15, 600, and 1,500 µg gemfibrozil/L. Exposure to 1,500 µg gemfibrozil/L caused a modest, but not significant, reduction in fecundity. However, gemfibrozil had no consistent effect on plasma cholesterol, triglycerides, or sex steroids after 21 d of exposure. The present study showed no evidence for significant physiological or reproductive impacts of gemfibrozil at an environmentally relevant concentration of 1.5 µg/L.
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investigating compensation and recovery of fathead minnow Pimephales Promelas exposed to 17α ethynylestradiol with metabolite profiling
Environmental Science & Technology, 2008Co-Authors: Drew R Ekman, Daniel L Villeneuve, Kathleen M Jensen, Michael D Kahl, Gerald T. Ankley, Elizabeth J Durhan, Quincy Teng, Timothy W ColletteAbstract:1H NMR spectroscopy was used to profile metabolite changes in the livers of fathead minnows (Pimephales Promelas) exposed to the synthetic estrogen 17α-ethynylestradiol (EE2) via a continuous flow water exposure. Fish were exposed to either 10 or 100 ng EE2/L for 8 days, followed by an 8 day depuration phase. Livers were collected after days 1, 4, and 8 of the exposure, and at the end of the depuration phase. Analysis of polar extracts of the liver revealed a greater impact of EE2 on males than females, with metabolite profiles of the former assuming similarities with those of the females (i.e., feminization) early in the exposure. Biochemical effects observed in the males included changes in metabolites relating to energetics (e.g., glycogen, glucose, and lactate) and liver toxicity (creatine and bile acids). In addition, amino acids associated with vitellogenin (VTG) synthesis increased in livers of EE2-exposed males, a finding consistent with increased plasma concentrations of the lipoprotein in the fi...
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investigating compensation and recovery of fathead minnow Pimephales Promelas exposed to 17α ethynylestradiol with metabolite profiling
Environmental Science & Technology, 2008Co-Authors: Drew R Ekman, Daniel L Villeneuve, Kathleen M Jensen, Michael D Kahl, Gerald T. Ankley, Elizabeth J Durhan, Quincy Teng, Timothy W ColletteAbstract:1H NMR spectroscopy was used to profile metabolite changes in the livers of fathead minnows (Pimephales Promelas) exposed to the synthetic estrogen 17alpha-ethynylestradiol (EE2) via a continuous flow water exposure. Fish were exposed to either 10 or 100 ng EE2/L for 8 days, followed by an 8 day depuration phase. Livers were collected after days 1, 4, and 8 of the exposure, and at the end of the depuration phase. Analysis of polar extracts of the liver revealed a greater impact of EE2 on males than females, with metabolite profiles of the former assuming similarities with those of the females (i.e., feminization) early in the exposure. Biochemical effects observed in the males included changes in metabolites relating to energetics (e.g., glycogen, glucose, and lactate) and liver toxicity (creatine and bile acids). In addition, amino acids associated with vitellogenin (VTG) synthesis increased in livers of EE2-exposed males, a finding consistent with increased plasma concentrations of the lipoprotein in the fish. Using partial least-squares discriminant analysis (PLS-DA), the response trajectories of the males at both exposure concentrations were compared. This revealed an apparent ability of the fish to compensate for the presence of EE2 later in the exposure, and to partially recover from its effects after the chemical was removed.
Michael D Kahl - One of the best experts on this subject based on the ideXlab platform.
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propiconazole inhibits steroidogenesis and reproduction in the fathead minnow Pimephales Promelas
Chemical Hazards in Industry, 2013Co-Authors: Sarah Y Skolness, Rodney D Johnson, Kathleen M Jensen, Michael D Kahl, Elizabeth A Makynen, Elizabeth J Durhan, Jenna E. Cavallin, Chad Blanksma, Jessica J Churchill, Daniel L VilleneuveAbstract:Conazoles are designed to inhibit cytochrome P450 (CYP) 14α-demethylase, an enzyme key to fungal cell wall formation. In vertebrates, conazoles may inhibit other CYPs, potentially disrupting processes like sex steroid synthesis. Propiconazole is a current-use pesticide that is among the first chemicals being tested in the U.S. Environmental Protection Agency endocrine disruptor screening program. Fathead minnows (Pimephales Promelas) were exposed to 0, 5, 50, 500, or 1000 µg propiconazole/l in a 21-day study that evaluated apical reproductive endpoints (fecundity, fertility, hatch); measures of endocrine function and steroid synthesis, such as cholesterol, vitellogenin (VTG), and sex steroid (testosterone (T), 17β-estradiol (E2)) concentrations in the plasma; and changes in gonadal expression of steroidogenic genes. Plasma E2 and VTG concentrations in females were reduced by exposure to propiconazole, and egg production was decreased in the 500 and 1000 µg/l treatment groups. These in vivo effects coincided with inhibition of E2 synthesis by ovary explants exposed to propiconazole in vitro. The authors also observed a compensatory response in females exposed to propiconazole, manifested as increased gonad weight and upregulation of genes coding for key steriodogenic proteins, including CYP19 (aromatase), CYP17 (hydroxylase/lyase), CYP11A (cholesterol side-chain-cleavage), and steroidogenic acute regulatory protein. Other than an increase in relative testis weight, effects on endocrine function in males were less pronounced than in females. This study provides important data relative to the potential endocrine activity of propiconazole in fish and, more generally, to the further delineation of pathways for the reproductive effects of steroid synthesis inhibitors in fish.
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effects of gemfibrozil on lipid metabolism steroidogenesis and reproduction in the fathead minnow Pimephales Promelas
Environmental Toxicology and Chemistry, 2012Co-Authors: Sarah Y Skolness, Daniel L Villeneuve, Kathleen M Jensen, Michael D Kahl, Elizabeth A Makynen, Elizabeth J Durhan, Gerald T. AnkleyAbstract:Fibrates are a class of pharmaceuticals that indirectly modulate cholesterol biosynthesis through effects on peroxisome proliferator-activated receptors. Gemfibrozil is a fibrate that has been detected in wastewater treatment plant influents, effluents, and drinking water. The objective of the present study was to assess the potential physiological and reproductive impacts of gemfibrozil on fathead minnows (Pimephales Promelas). Fish were exposed to gemfibrozil in two different studies. The first was a short-term test with water concentrations of 0, 15, and 600 µg gemfibrozil/L, sampling after 2 or 8 d of exposure. Plasma cholesterol concentrations were significantly reduced in males exposed to 600 µg gemfibrozil/L for 8 d. In addition, expression of several hepatic genes important to lipid metabolism was altered, suggesting that gemfibrozil does affect lipid metabolism in fish. A 21-d study was conducted to investigate further the effects on lipid metabolism and steroidogenesis as well as to assess potential impacts of gemfibrozil on reproduction. Fish were exposed to water concentrations of 0, 1.5, 15, 600, and 1,500 µg gemfibrozil/L. Exposure to 1,500 µg gemfibrozil/L caused a modest, but not significant, reduction in fecundity. However, gemfibrozil had no consistent effect on plasma cholesterol, triglycerides, or sex steroids after 21 d of exposure. The present study showed no evidence for significant physiological or reproductive impacts of gemfibrozil at an environmentally relevant concentration of 1.5 µg/L. Environ. Toxicol. Chem. 2012; 31: 2615–2624. © 2012 SETAC
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effects of gemfibrozil on lipid metabolism steroidogenesis and reproduction in the fathead minnow Pimephales Promelas
Environmental Toxicology and Chemistry, 2012Co-Authors: Sarah Y Skolness, Daniel L Villeneuve, Kathleen M Jensen, Michael D Kahl, Elizabeth A Makynen, Elizabeth J Durhan, Gerald T. AnkleyAbstract:Fibrates are a class of pharmaceuticals that indirectly modulate cholesterol biosynthesis through effects on peroxisome proliferator-activated receptors. Gemfibrozil is a fibrate that has been detected in wastewater treatment plant influents, effluents, and drinking water. The objective of the present study was to assess the potential physiological and reproductive impacts of gemfibrozil on fathead minnows (Pimephales Promelas). Fish were exposed to gemfibrozil in two different studies. The first was a short-term test with water concentrations of 0, 15, and 600 µg gemfibrozil/L, sampling after 2 or 8 d of exposure. Plasma cholesterol concentrations were significantly reduced in males exposed to 600 µg gemfibrozil/L for 8 d. In addition, expression of several hepatic genes important to lipid metabolism was altered, suggesting that gemfibrozil does affect lipid metabolism in fish. A 21-d study was conducted to investigate further the effects on lipid metabolism and steroidogenesis as well as to assess potential impacts of gemfibrozil on reproduction. Fish were exposed to water concentrations of 0, 1.5, 15, 600, and 1,500 µg gemfibrozil/L. Exposure to 1,500 µg gemfibrozil/L caused a modest, but not significant, reduction in fecundity. However, gemfibrozil had no consistent effect on plasma cholesterol, triglycerides, or sex steroids after 21 d of exposure. The present study showed no evidence for significant physiological or reproductive impacts of gemfibrozil at an environmentally relevant concentration of 1.5 µg/L.
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Influence of ovarian stage on transcript profiles in fathead minnow (Pimephales Promelas) ovary tissue
Aquatic toxicology (Amsterdam Netherlands), 2010Co-Authors: Daniel L Villeneuve, Michael D Kahl, Natàlia Garcia-reyero, Dalma Martinović, Jenna E. Cavallin, Nathaniel D. Mueller, Leah C. Wehmas, Anne L. Linnum, Edward J. Perkins, Gerald T. AnkleyAbstract:Interpretation of toxicogenomic experiments conducted with ovary tissue from asynchronous-spawning small fish species is complicated by background variation in the relative abundance and proportion of follicles at different stages within the ovary tissue sample. This study employed both real-time quantitative polymerase chain reaction and a 15,000 gene oligonucleotide microarray to examine variation in the fathead minnow (Pimephales Promelas) ovarian transcriptional profile as a function of quantitative and qualitative differences in ovarian histology. The objectives were to provide data that could potentially aid interpretation of future toxicogenomics experiments, identify putative stage-related transcriptional markers, and generate insights into basic biological regulation of asynchronous oocyte development. Multiple lines of evidence from the present study indicate that variation in the transcriptional profile is primarily dependent on the relative abundance of previtellogenic versus vitellogenic follicles in the ovary. Due to the relatively small proportions of mature ovulated follicles or atretic follicles in the overall follicle population, few potential transcriptional markers of maturation, ovulation, or atresia could be identified. However, among the 460 differentially expressed genes identified in the present study, several targets, including HtrA serine peptidase 3 (htra3), tissue inhibitor of metalloproteinase 3 (timp3), aquaporin 8 (aqp8), transgelin 2 like (tagln2), Nedd4 family interacting protein 2 (ndfip2), chemokine ligand 12a (cxcl12a), midkine-related growth factor (mdka), and jagged 1b (jag 1b) exhibited responses and functional properties that support them as candidate molecular markers of significant shift in gross ovarian stage. Genes associated with a diversity of functions including cellular development, morphogenesis, coated vesicle transport, sexual reproduction, and neuron development, among others, were statistically enriched within the list of 460 genes differentially expressed among different ovarian classes. Overall, results of this study provide insights into background variation in ovary transcript profiles that should aid and enhance the interpretation of toxicogenomic data generated in experiments conducted with small, asynchronous-spawning fish species.
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investigating compensation and recovery of fathead minnow Pimephales Promelas exposed to 17α ethynylestradiol with metabolite profiling
Environmental Science & Technology, 2008Co-Authors: Drew R Ekman, Daniel L Villeneuve, Kathleen M Jensen, Michael D Kahl, Gerald T. Ankley, Elizabeth J Durhan, Quincy Teng, Timothy W ColletteAbstract:1H NMR spectroscopy was used to profile metabolite changes in the livers of fathead minnows (Pimephales Promelas) exposed to the synthetic estrogen 17α-ethynylestradiol (EE2) via a continuous flow water exposure. Fish were exposed to either 10 or 100 ng EE2/L for 8 days, followed by an 8 day depuration phase. Livers were collected after days 1, 4, and 8 of the exposure, and at the end of the depuration phase. Analysis of polar extracts of the liver revealed a greater impact of EE2 on males than females, with metabolite profiles of the former assuming similarities with those of the females (i.e., feminization) early in the exposure. Biochemical effects observed in the males included changes in metabolites relating to energetics (e.g., glycogen, glucose, and lactate) and liver toxicity (creatine and bile acids). In addition, amino acids associated with vitellogenin (VTG) synthesis increased in livers of EE2-exposed males, a finding consistent with increased plasma concentrations of the lipoprotein in the fi...
Robert A Hoke - One of the best experts on this subject based on the ideXlab platform.
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sequencing and de novo draft assemblies of a fathead minnow Pimephales Promelas reference genome
Environmental Toxicology and Chemistry, 2016Co-Authors: Frank R Burns, Daniel L Villeneuve, Gerald T. Ankley, Amarin L Cogburn, Eric R Waits, Yunjuan Chang, Victor Llaca, Stephane Deschamps, Raymond E Jackson, Robert A HokeAbstract:The present study was undertaken to provide the foundation for development of genome-scale resources for the fathead minnow (Pimephales Promelas), an important model organism widely used in both aquatic toxicology research and regulatory testing. The authors report on the first sequencing and 2 draft assemblies for the reference genome of this species. Approximately 120× sequence coverage was achieved via Illumina sequencing of a combination of paired-end, mate-pair, and fosmid libraries. Evaluation and comparison of these assemblies demonstrate that they are of sufficient quality to be useful for genome-enabled studies, with 418 of 458 (91%) conserved eukaryotic genes mapping to at least 1 of the assemblies. In addition to its immediate utility, the present work provides a strong foundation on which to build further refinements of a reference genome for the fathead minnow.
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sequencing and de novo draft assemblies of a fathead minnow Pimephales Promelas reference genome
Environmental Toxicology and Chemistry, 2016Co-Authors: Frank R Burns, Daniel L Villeneuve, Gerald T. Ankley, Amarin L Cogburn, Eric R Waits, Yunjuan Chang, Victor Llaca, Stephane Deschamps, Raymond E Jackson, Robert A HokeAbstract:The present study was undertaken to provide the foundation for development of genome-scale resources for the fathead minnow (Pimephales Promelas), an important model organism widely used in both aquatic toxicology research and regulatory testing. The authors report on the first sequencing and 2 draft assemblies for the reference genome of this species. Approximately 120× sequence coverage was achieved via Illumina sequencing of a combination of paired-end, mate-pair, and fosmid libraries. Evaluation and comparison of these assemblies demonstrate that they are of sufficient quality to be useful for genome-enabled studies, with 418 of 458 (91%) conserved eukaryotic genes mapping to at least 1 of the assemblies. In addition to its immediate utility, the present work provides a strong foundation on which to build further refinements of a reference genome for the fathead minnow. Environ Toxicol Chem 2016;35:212–217. © 2015 SETAC