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Abderrazak Maaroufi - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of Pistacia lentiscus seed oil and phenolic compounds for in vitro antiproliferative effects against BHK21 cells
Pharmaceutical Biology, 2016Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abdelhamid Khaldi, Sarra Shili, Nejia Ben Ali, Abderrazak MaaroufiAbstract:Context: Within the global context of increasing cancer diseases, natural products are important in devising new drugs and providing unique ideas in cancer therapy. In Tunisian folk medicine, Pistacia lentiscus L. (Anacardiaceae) fixed oil is used for cancer treatment. Objective: This investigation studied, for the first time, the antiproliferative effect of Pistacia lentiscus fixed oil and its phenolic extract on BHK21 cancer cells. Materials and methods: Oil was extracted from fruits harvested in northwest Tunisia and the phenolic fraction was obtained by mixing with methanol. The anti-proliferative activity of the two tested substances on BHK 21 cells were investigated in vitro using trypan blue assays. Cells were treated with different concentrations of P. lentiscus oil (0.009, 0.018, 0.036, and 0.09 g/mL) and the phenolic extract (0.007, 0.014, 0.03, and 0.07 g/mL) for 24, 48, and 72 h. Results: The inhibitory effect of Pistacia lentiscus fixed oil increases with the increase in dose. The IC50 value was estimated at 0.029 g/mL. The percentage of cell viability was 42.46 ± 3.4% at a dose of 0.09 g/mL and was significantly lower than that of the untreated control (96.24 ± 2.5%, p
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Evaluation of Pistacia lentiscus seed oil and phenolic compounds for in vitro antiproliferative effects against BHK21 cells
Pharmaceutical biology, 2015Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abdelhamid Khaldi, Sarra Shili, Nejia Ben Ali, Abderrazak MaaroufiAbstract:AbstractContext: Within the global context of increasing cancer diseases, natural products are important in devising new drugs and providing unique ideas in cancer therapy. In Tunisian folk medicine, Pistacia lentiscus L. (Anacardiaceae) fixed oil is used for cancer treatment.Objective: This investigation studied, for the first time, the antiproliferative effect of Pistacia lentiscus fixed oil and its phenolic extract on BHK21 cancer cells.Materials and methods: Oil was extracted from fruits harvested in northwest Tunisia and the phenolic fraction was obtained by mixing with methanol. The anti-proliferative activity of the two tested substances on BHK 21 cells were investigated in vitro using trypan blue assays. Cells were treated with different concentrations of P. lentiscus oil (0.009, 0.018, 0.036, and 0.09 g/mL) and the phenolic extract (0.007, 0.014, 0.03, and 0.07 g/mL) for 24, 48, and 72 h.Results: The inhibitory effect of Pistacia lentiscus fixed oil increases with the increase in dose. The IC50 v...
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In vitro antimicrobial activity of Pistacia lentiscus L. edible oil and phenolic extract.
Natural Product Research, 2015Co-Authors: F. Mezni, C. Aouadhi, M L Khouja, A Khaldi, Abderrazak MaaroufiAbstract:Pistacia lentiscus L. is known in some Tunisian forest area by its fixed oil used in traditional medicine as an antiseptic product. This investigation is the first to study the antimicrobial activity of P.lentiscus edible oil and its phenolic extract. Oil was extracted from fruits harvested from six provenances located in Tunisia. The antimicrobial activity was tested using disc diffusion assay and the broth dilution method. Kbouch and Sidi Zid oils were most efficient (p
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in vitro antimicrobial activity of Pistacia lentiscus l edible oil and phenolic extract
Natural Product Research, 2015Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abdelhamid Khaldi, C. Aouadhi, Abderrazak MaaroufiAbstract:Pistacia lentiscus L. is known in some Tunisian forest area by its fixed oil used in traditional medicine as an antiseptic product. This investigation is the first to study the antimicrobial activity of P.lentiscus edible oil and its phenolic extract. Oil was extracted from fruits harvested from six provenances located in Tunisia. The antimicrobial activity was tested using disc diffusion assay and the broth dilution method. Kbouch and Sidi Zid oils were most efficient (p < 0.003) against, respectively, Staphylococcus aureus and Aspergillus niger with an inhibition zone of 9.33 mm. The phenolic extract had the largest spectrum of sensitive microorganisms. The minimum inhibitory concentration and minimum bactericidal concentration results showed that all strains were inhibited by both oil and extract.
Faten Mezni - One of the best experts on this subject based on the ideXlab platform.
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Diversity of Sterol Composition in Tunisian Pistacia lentiscus Seed Oil
Chemistry and Biodiversity, 2016Co-Authors: Faten Mezni, Arbia Labidi, Mohamed Larbi Khouja, Lucy Martine, Olivier Berdeaux, Abdelhamid KhaldiAbstract:Pistacia lentiscus L. seed oil is used in some Mediterranean forest area for culinary and medicinal purposes. In this study, we aim to examine, for the first time, the effect of growing area on sterol content of Pistacia lentiscus seed oil. Fruits were harvested from 13 different sites located in northern and central Tunisia. Gas chromatography-flame-ionization detection (GC-FID) was used to quantify sterols and gas chromatography/mass spectrometry (GC/MS) was used to identify them. The major sterol identified was b-sitosterol with a value ranging from 854.12 to 1224.09 mg/kg of oil, thus making up more than 54% of the total sterols. The other two main sterols were cycloartenol (11%) and 24-methylene-cycloartenol (5%). Statistical results revealed that growing location significantly (P < 0.001) affected phytosterol levels in these oils.
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Evaluation of Pistacia lentiscus seed oil and phenolic compounds for in vitro antiproliferative effects against BHK21 cells
Pharmaceutical Biology, 2016Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abdelhamid Khaldi, Sarra Shili, Nejia Ben Ali, Abderrazak MaaroufiAbstract:Context: Within the global context of increasing cancer diseases, natural products are important in devising new drugs and providing unique ideas in cancer therapy. In Tunisian folk medicine, Pistacia lentiscus L. (Anacardiaceae) fixed oil is used for cancer treatment. Objective: This investigation studied, for the first time, the antiproliferative effect of Pistacia lentiscus fixed oil and its phenolic extract on BHK21 cancer cells. Materials and methods: Oil was extracted from fruits harvested in northwest Tunisia and the phenolic fraction was obtained by mixing with methanol. The anti-proliferative activity of the two tested substances on BHK 21 cells were investigated in vitro using trypan blue assays. Cells were treated with different concentrations of P. lentiscus oil (0.009, 0.018, 0.036, and 0.09 g/mL) and the phenolic extract (0.007, 0.014, 0.03, and 0.07 g/mL) for 24, 48, and 72 h. Results: The inhibitory effect of Pistacia lentiscus fixed oil increases with the increase in dose. The IC50 value was estimated at 0.029 g/mL. The percentage of cell viability was 42.46 ± 3.4% at a dose of 0.09 g/mL and was significantly lower than that of the untreated control (96.24 ± 2.5%, p
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Evaluation of Pistacia lentiscus seed oil and phenolic compounds for in vitro antiproliferative effects against BHK21 cells
Pharmaceutical biology, 2015Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abdelhamid Khaldi, Sarra Shili, Nejia Ben Ali, Abderrazak MaaroufiAbstract:AbstractContext: Within the global context of increasing cancer diseases, natural products are important in devising new drugs and providing unique ideas in cancer therapy. In Tunisian folk medicine, Pistacia lentiscus L. (Anacardiaceae) fixed oil is used for cancer treatment.Objective: This investigation studied, for the first time, the antiproliferative effect of Pistacia lentiscus fixed oil and its phenolic extract on BHK21 cancer cells.Materials and methods: Oil was extracted from fruits harvested in northwest Tunisia and the phenolic fraction was obtained by mixing with methanol. The anti-proliferative activity of the two tested substances on BHK 21 cells were investigated in vitro using trypan blue assays. Cells were treated with different concentrations of P. lentiscus oil (0.009, 0.018, 0.036, and 0.09 g/mL) and the phenolic extract (0.007, 0.014, 0.03, and 0.07 g/mL) for 24, 48, and 72 h.Results: The inhibitory effect of Pistacia lentiscus fixed oil increases with the increase in dose. The IC50 v...
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in vitro antimicrobial activity of Pistacia lentiscus l edible oil and phenolic extract
Natural Product Research, 2015Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abdelhamid Khaldi, C. Aouadhi, Abderrazak MaaroufiAbstract:Pistacia lentiscus L. is known in some Tunisian forest area by its fixed oil used in traditional medicine as an antiseptic product. This investigation is the first to study the antimicrobial activity of P.lentiscus edible oil and its phenolic extract. Oil was extracted from fruits harvested from six provenances located in Tunisia. The antimicrobial activity was tested using disc diffusion assay and the broth dilution method. Kbouch and Sidi Zid oils were most efficient (p < 0.003) against, respectively, Staphylococcus aureus and Aspergillus niger with an inhibition zone of 9.33 mm. The phenolic extract had the largest spectrum of sensitive microorganisms. The minimum inhibitory concentration and minimum bactericidal concentration results showed that all strains were inhibited by both oil and extract.
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Fatty acid composition, antioxidant and antibacterial activities of Pistacia lentiscus L. fruit oils
Journal of Medicinal Plants Research, 2012Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abderrazzek Maaroufi, Mongi Msallem, Mohamed Boussaid, Abdelhamid KhaldiAbstract:This study was performed on oil extracted from mature fruits of Pistacia lentiscus L. harvested from Northern and North Western Tunisia. Extraction was done by two methods: Traditional method practiced by women in forest areas and pressing method that was proposed to improve the yield and the quality of oil. Fatty acid composition was determined by gas chromatography-mass spectrometry (GC/MS). Oleic acid was the main fatty acid with more than 56%, followed by palmitic with 27%. Antioxidant activity of these oils was improved by pressing method; the percentage of inhibition of DPPH was increased from 21 to 43% for Nefza provenance and from 19 to 29% for Bizerte one. Similarly, the highest values of trolox equivalent antioxidant capacity (TEAC) were reached by oils extracted by pressing method. Antibacterial activity was tested against three strains: Escherichia coli,Salmonella typhimurium and Clostridium perfringens. The results showed the existence of a significant bactericidal effect in the case of C. perfringens for oils from Bizerte with an inhibition diameter of about 13 mm for the oil extracted by pressing method and 8 mm for that extracted by traditional one. The effect was not significant in the case of E. coli and S. typhimurium. Key words: Pistacia lentiscus L., fixed oil, fatty acids, antioxidant activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH), trolox equivalent antioxidant capacity (TEAC), antibacterial activity.
Sofia Mitaku - One of the best experts on this subject based on the ideXlab platform.
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chemical composition and antimicrobial activity of the essential oils of Pistacia lentiscus var chia
Planta Medica, 1999Co-Authors: Prokopios Magiatis, Eleni Melliou, Alexiosleandros Skaltsounis, Ioanna Chinou, Sofia MitakuAbstract:The chemical composition of the three essential oils obtained by steam distillation of the mastic gum, leaves and twigs of Pistacia lentiscus var. chia, was studied by GC/MS. Sixty nine constituents were identified from the oils. α-Pinene, myrcene, trans-caryophyllene and germacrene D were found to be the major components. The in vitro antimicrobial activity of the three essential oils and of the resin (total, acid and neutral fraction) against six bacteria and three fungi is reported.
Abdelhamid Khaldi - One of the best experts on this subject based on the ideXlab platform.
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Diversity of Sterol Composition in Tunisian Pistacia lentiscus Seed Oil
Chemistry and Biodiversity, 2016Co-Authors: Faten Mezni, Arbia Labidi, Mohamed Larbi Khouja, Lucy Martine, Olivier Berdeaux, Abdelhamid KhaldiAbstract:Pistacia lentiscus L. seed oil is used in some Mediterranean forest area for culinary and medicinal purposes. In this study, we aim to examine, for the first time, the effect of growing area on sterol content of Pistacia lentiscus seed oil. Fruits were harvested from 13 different sites located in northern and central Tunisia. Gas chromatography-flame-ionization detection (GC-FID) was used to quantify sterols and gas chromatography/mass spectrometry (GC/MS) was used to identify them. The major sterol identified was b-sitosterol with a value ranging from 854.12 to 1224.09 mg/kg of oil, thus making up more than 54% of the total sterols. The other two main sterols were cycloartenol (11%) and 24-methylene-cycloartenol (5%). Statistical results revealed that growing location significantly (P < 0.001) affected phytosterol levels in these oils.
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Evaluation of Pistacia lentiscus seed oil and phenolic compounds for in vitro antiproliferative effects against BHK21 cells
Pharmaceutical Biology, 2016Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abdelhamid Khaldi, Sarra Shili, Nejia Ben Ali, Abderrazak MaaroufiAbstract:Context: Within the global context of increasing cancer diseases, natural products are important in devising new drugs and providing unique ideas in cancer therapy. In Tunisian folk medicine, Pistacia lentiscus L. (Anacardiaceae) fixed oil is used for cancer treatment. Objective: This investigation studied, for the first time, the antiproliferative effect of Pistacia lentiscus fixed oil and its phenolic extract on BHK21 cancer cells. Materials and methods: Oil was extracted from fruits harvested in northwest Tunisia and the phenolic fraction was obtained by mixing with methanol. The anti-proliferative activity of the two tested substances on BHK 21 cells were investigated in vitro using trypan blue assays. Cells were treated with different concentrations of P. lentiscus oil (0.009, 0.018, 0.036, and 0.09 g/mL) and the phenolic extract (0.007, 0.014, 0.03, and 0.07 g/mL) for 24, 48, and 72 h. Results: The inhibitory effect of Pistacia lentiscus fixed oil increases with the increase in dose. The IC50 value was estimated at 0.029 g/mL. The percentage of cell viability was 42.46 ± 3.4% at a dose of 0.09 g/mL and was significantly lower than that of the untreated control (96.24 ± 2.5%, p
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Evaluation of Pistacia lentiscus seed oil and phenolic compounds for in vitro antiproliferative effects against BHK21 cells
Pharmaceutical biology, 2015Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abdelhamid Khaldi, Sarra Shili, Nejia Ben Ali, Abderrazak MaaroufiAbstract:AbstractContext: Within the global context of increasing cancer diseases, natural products are important in devising new drugs and providing unique ideas in cancer therapy. In Tunisian folk medicine, Pistacia lentiscus L. (Anacardiaceae) fixed oil is used for cancer treatment.Objective: This investigation studied, for the first time, the antiproliferative effect of Pistacia lentiscus fixed oil and its phenolic extract on BHK21 cancer cells.Materials and methods: Oil was extracted from fruits harvested in northwest Tunisia and the phenolic fraction was obtained by mixing with methanol. The anti-proliferative activity of the two tested substances on BHK 21 cells were investigated in vitro using trypan blue assays. Cells were treated with different concentrations of P. lentiscus oil (0.009, 0.018, 0.036, and 0.09 g/mL) and the phenolic extract (0.007, 0.014, 0.03, and 0.07 g/mL) for 24, 48, and 72 h.Results: The inhibitory effect of Pistacia lentiscus fixed oil increases with the increase in dose. The IC50 v...
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in vitro antimicrobial activity of Pistacia lentiscus l edible oil and phenolic extract
Natural Product Research, 2015Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abdelhamid Khaldi, C. Aouadhi, Abderrazak MaaroufiAbstract:Pistacia lentiscus L. is known in some Tunisian forest area by its fixed oil used in traditional medicine as an antiseptic product. This investigation is the first to study the antimicrobial activity of P.lentiscus edible oil and its phenolic extract. Oil was extracted from fruits harvested from six provenances located in Tunisia. The antimicrobial activity was tested using disc diffusion assay and the broth dilution method. Kbouch and Sidi Zid oils were most efficient (p < 0.003) against, respectively, Staphylococcus aureus and Aspergillus niger with an inhibition zone of 9.33 mm. The phenolic extract had the largest spectrum of sensitive microorganisms. The minimum inhibitory concentration and minimum bactericidal concentration results showed that all strains were inhibited by both oil and extract.
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Fatty acid composition, antioxidant and antibacterial activities of Pistacia lentiscus L. fruit oils
Journal of Medicinal Plants Research, 2012Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abderrazzek Maaroufi, Mongi Msallem, Mohamed Boussaid, Abdelhamid KhaldiAbstract:This study was performed on oil extracted from mature fruits of Pistacia lentiscus L. harvested from Northern and North Western Tunisia. Extraction was done by two methods: Traditional method practiced by women in forest areas and pressing method that was proposed to improve the yield and the quality of oil. Fatty acid composition was determined by gas chromatography-mass spectrometry (GC/MS). Oleic acid was the main fatty acid with more than 56%, followed by palmitic with 27%. Antioxidant activity of these oils was improved by pressing method; the percentage of inhibition of DPPH was increased from 21 to 43% for Nefza provenance and from 19 to 29% for Bizerte one. Similarly, the highest values of trolox equivalent antioxidant capacity (TEAC) were reached by oils extracted by pressing method. Antibacterial activity was tested against three strains: Escherichia coli,Salmonella typhimurium and Clostridium perfringens. The results showed the existence of a significant bactericidal effect in the case of C. perfringens for oils from Bizerte with an inhibition diameter of about 13 mm for the oil extracted by pressing method and 8 mm for that extracted by traditional one. The effect was not significant in the case of E. coli and S. typhimurium. Key words: Pistacia lentiscus L., fixed oil, fatty acids, antioxidant activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH), trolox equivalent antioxidant capacity (TEAC), antibacterial activity.
Mohamed Larbi Khouja - One of the best experts on this subject based on the ideXlab platform.
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Diversity of Sterol Composition in Tunisian Pistacia lentiscus Seed Oil
Chemistry and Biodiversity, 2016Co-Authors: Faten Mezni, Arbia Labidi, Mohamed Larbi Khouja, Lucy Martine, Olivier Berdeaux, Abdelhamid KhaldiAbstract:Pistacia lentiscus L. seed oil is used in some Mediterranean forest area for culinary and medicinal purposes. In this study, we aim to examine, for the first time, the effect of growing area on sterol content of Pistacia lentiscus seed oil. Fruits were harvested from 13 different sites located in northern and central Tunisia. Gas chromatography-flame-ionization detection (GC-FID) was used to quantify sterols and gas chromatography/mass spectrometry (GC/MS) was used to identify them. The major sterol identified was b-sitosterol with a value ranging from 854.12 to 1224.09 mg/kg of oil, thus making up more than 54% of the total sterols. The other two main sterols were cycloartenol (11%) and 24-methylene-cycloartenol (5%). Statistical results revealed that growing location significantly (P < 0.001) affected phytosterol levels in these oils.
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Evaluation of Pistacia lentiscus seed oil and phenolic compounds for in vitro antiproliferative effects against BHK21 cells
Pharmaceutical Biology, 2016Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abdelhamid Khaldi, Sarra Shili, Nejia Ben Ali, Abderrazak MaaroufiAbstract:Context: Within the global context of increasing cancer diseases, natural products are important in devising new drugs and providing unique ideas in cancer therapy. In Tunisian folk medicine, Pistacia lentiscus L. (Anacardiaceae) fixed oil is used for cancer treatment. Objective: This investigation studied, for the first time, the antiproliferative effect of Pistacia lentiscus fixed oil and its phenolic extract on BHK21 cancer cells. Materials and methods: Oil was extracted from fruits harvested in northwest Tunisia and the phenolic fraction was obtained by mixing with methanol. The anti-proliferative activity of the two tested substances on BHK 21 cells were investigated in vitro using trypan blue assays. Cells were treated with different concentrations of P. lentiscus oil (0.009, 0.018, 0.036, and 0.09 g/mL) and the phenolic extract (0.007, 0.014, 0.03, and 0.07 g/mL) for 24, 48, and 72 h. Results: The inhibitory effect of Pistacia lentiscus fixed oil increases with the increase in dose. The IC50 value was estimated at 0.029 g/mL. The percentage of cell viability was 42.46 ± 3.4% at a dose of 0.09 g/mL and was significantly lower than that of the untreated control (96.24 ± 2.5%, p
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Evaluation of Pistacia lentiscus seed oil and phenolic compounds for in vitro antiproliferative effects against BHK21 cells
Pharmaceutical biology, 2015Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abdelhamid Khaldi, Sarra Shili, Nejia Ben Ali, Abderrazak MaaroufiAbstract:AbstractContext: Within the global context of increasing cancer diseases, natural products are important in devising new drugs and providing unique ideas in cancer therapy. In Tunisian folk medicine, Pistacia lentiscus L. (Anacardiaceae) fixed oil is used for cancer treatment.Objective: This investigation studied, for the first time, the antiproliferative effect of Pistacia lentiscus fixed oil and its phenolic extract on BHK21 cancer cells.Materials and methods: Oil was extracted from fruits harvested in northwest Tunisia and the phenolic fraction was obtained by mixing with methanol. The anti-proliferative activity of the two tested substances on BHK 21 cells were investigated in vitro using trypan blue assays. Cells were treated with different concentrations of P. lentiscus oil (0.009, 0.018, 0.036, and 0.09 g/mL) and the phenolic extract (0.007, 0.014, 0.03, and 0.07 g/mL) for 24, 48, and 72 h.Results: The inhibitory effect of Pistacia lentiscus fixed oil increases with the increase in dose. The IC50 v...
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in vitro antimicrobial activity of Pistacia lentiscus l edible oil and phenolic extract
Natural Product Research, 2015Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abdelhamid Khaldi, C. Aouadhi, Abderrazak MaaroufiAbstract:Pistacia lentiscus L. is known in some Tunisian forest area by its fixed oil used in traditional medicine as an antiseptic product. This investigation is the first to study the antimicrobial activity of P.lentiscus edible oil and its phenolic extract. Oil was extracted from fruits harvested from six provenances located in Tunisia. The antimicrobial activity was tested using disc diffusion assay and the broth dilution method. Kbouch and Sidi Zid oils were most efficient (p < 0.003) against, respectively, Staphylococcus aureus and Aspergillus niger with an inhibition zone of 9.33 mm. The phenolic extract had the largest spectrum of sensitive microorganisms. The minimum inhibitory concentration and minimum bactericidal concentration results showed that all strains were inhibited by both oil and extract.
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Fatty acid composition, antioxidant and antibacterial activities of Pistacia lentiscus L. fruit oils
Journal of Medicinal Plants Research, 2012Co-Authors: Faten Mezni, Mohamed Larbi Khouja, Abderrazzek Maaroufi, Mongi Msallem, Mohamed Boussaid, Abdelhamid KhaldiAbstract:This study was performed on oil extracted from mature fruits of Pistacia lentiscus L. harvested from Northern and North Western Tunisia. Extraction was done by two methods: Traditional method practiced by women in forest areas and pressing method that was proposed to improve the yield and the quality of oil. Fatty acid composition was determined by gas chromatography-mass spectrometry (GC/MS). Oleic acid was the main fatty acid with more than 56%, followed by palmitic with 27%. Antioxidant activity of these oils was improved by pressing method; the percentage of inhibition of DPPH was increased from 21 to 43% for Nefza provenance and from 19 to 29% for Bizerte one. Similarly, the highest values of trolox equivalent antioxidant capacity (TEAC) were reached by oils extracted by pressing method. Antibacterial activity was tested against three strains: Escherichia coli,Salmonella typhimurium and Clostridium perfringens. The results showed the existence of a significant bactericidal effect in the case of C. perfringens for oils from Bizerte with an inhibition diameter of about 13 mm for the oil extracted by pressing method and 8 mm for that extracted by traditional one. The effect was not significant in the case of E. coli and S. typhimurium. Key words: Pistacia lentiscus L., fixed oil, fatty acids, antioxidant activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH), trolox equivalent antioxidant capacity (TEAC), antibacterial activity.