The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Norbert Heimburger - One of the best experts on this subject based on the ideXlab platform.
-
Isolation of plasminogen activator inhibitor-2 (PAI-2) from human Placenta. Evidence for vitronectin/PAI-2 complexes in human Placenta Extract.
Biological chemistry Hoppe-Seyler, 1990Co-Authors: Klaus-p. Radtke, Karl-h. Wenz, Norbert HeimburgerAbstract:Plasminogen activator inhibitor-2 (PAI-2), found in human Placenta and pregnancy plasma, was prepared in a highly purified and functionally active form from human Placenta. The purification was achieved by a combination of Rivanol and ammonium sulfate precipitation, followed by chromatography on DEAE Affigel Blue, hydroxylapatite and phenylalanine-Sepharose. PAI-2, which is precipitated by low Rivanol concentrations, can be selectively redissolved from the pellet by increasing the Rivanol concentration in the presence of a reducing agent, i.e. dithiothreitol. The purified protein shows a molecular mass of 45 kDa in SDS PAGE, cross-reacts with monoclonal antibodies against PAI-2 (Mab'PAI-2), and inhibits the amidolytic activity of urokinase-type plasminogen activator (u-PA) towards the chromogenic substrate Glu-Gly-Arg-pNA (S-2444). The specific activity of the purified inhibitor was 52,300 units/mg, attaining 71,000 units/mg in peak fractions. In the immunopurification of Placental Extract on anti-PAI-2 Sepharose, the eluate showed the expected reaction with Mab' PAI-2, and it also cross-reacted with anti-vitronectin serum. In order to complement these results, anti-vitronectin Sepharose was used for immunopurification of Placenta Extract. In Western Blot experiments the eluates of anti PAI-2 Sepharose and anti-vitronectin Sepharose both showed a heterogeneous pattern of high molecular weight bands recognized by either polyclonal antiserum against vitronectin or Mab'PAI-2. In either case, reduction of the eluates releases mainly a 45-kDa band, which is recognized by Mab'PAI-2, or 80-kDa and 76-kDa bands recognized by anti-serum against vitronectin. These data suggest that the predominant form of PAI-2 in Placenta Extract is heterogeneous and of high molecular mass, containing complexes in which vitronectin is covalently bound to PAI-2 by disulfide bridges.
-
isolation of plasminogen activator inhibitor 2 pai 2 from human Placenta evidence for vitronectin pai 2 complexes in human Placenta Extract
Biological chemistry Hoppe-Seyler, 1990Co-Authors: Klaus-p. Radtke, Karl-h. Wenz, Norbert HeimburgerAbstract:Plasminogen activator inhibitor-2 (PAI-2), found in human Placenta and pregnancy plasma, was prepared in a highly purified and functionally active form from human Placenta. The purification was achieved by a combination of Rivanol and ammonium sulfate precipitation, followed by chromatography on DEAE Affigel Blue, hydroxylapatite and phenylalanine-Sepharose. PAI-2, which is precipitated by low Rivanol concentrations, can be selectively redissolved from the pellet by increasing the Rivanol concentration in the presence of a reducing agent, i.e. dithiothreitol. The purified protein shows a molecular mass of 45 kDa in SDS PAGE, cross-reacts with monoclonal antibodies against PAI-2 (Mab'PAI-2), and inhibits the amidolytic activity of urokinase-type plasminogen activator (u-PA) towards the chromogenic substrate Glu-Gly-Arg-pNA (S-2444). The specific activity of the purified inhibitor was 52,300 units/mg, attaining 71,000 units/mg in peak fractions. In the immunopurification of Placental Extract on anti-PAI-2 Sepharose, the eluate showed the expected reaction with Mab' PAI-2, and it also cross-reacted with anti-vitronectin serum. In order to complement these results, anti-vitronectin Sepharose was used for immunopurification of Placenta Extract. In Western Blot experiments the eluates of anti PAI-2 Sepharose and anti-vitronectin Sepharose both showed a heterogeneous pattern of high molecular weight bands recognized by either polyclonal antiserum against vitronectin or Mab'PAI-2. In either case, reduction of the eluates releases mainly a 45-kDa band, which is recognized by Mab'PAI-2, or 80-kDa and 76-kDa bands recognized by anti-serum against vitronectin. These data suggest that the predominant form of PAI-2 in Placenta Extract is heterogeneous and of high molecular mass, containing complexes in which vitronectin is covalently bound to PAI-2 by disulfide bridges.
Georgy A Nevinsky - One of the best experts on this subject based on the ideXlab platform.
-
RESEARCH ARTICLE Extremely Stable Soluble High Molecular Mass Multi-Protein Complex with DNase Activity in Human Placental Tissue
2016Co-Authors: Evgeniya E. Burkova, Svetlana E. Soboleva, Pavel S. Dmitrenok, Sergey E Sedykh, Valentina N, Georgy A NevinskyAbstract:Human Placenta is an organ which protects, feeds, and regulates the grooving of the embryo. Therefore, identification and characterization of Placental components including proteins and their multi-protein complexes is an important step to understanding the Placenta function. We have obtained and analyzed for the first time an extremely stable multi-protein complex (SPC,,1000 kDa) from the soluble fraction of three human Placentas. By gel filtration on Sepharose-4B, the SPC was well separated from other proteins of the Placenta Extract. Light scattering measurements and gel filtration showed that the SPC is stable in the presence of NaCl, MgCl2, acetonitrile, guanidinium chloride, and Triton in high concentrations, but dissociates efficiently in the presence of 8 M urea, 50 mM EDTA, and 0.5 M NaCl. Such a stable complex is unlikely to be a casual associate of different proteins. According to SDS-PAGE and MALDI mass spectrometry data, this complex contains many major glycosylated proteins with low and moderate molecular masses (MMs) 4–14 kDa and several moderately abundant (79.3, 68.5
-
Extremely stable soluble high molecular mass multi-protein complex with DNase activity in human Placental tissue.
PLOS ONE, 2014Co-Authors: Evgeniya E. Burkova, Valentina N. Buneva, Svetlana E. Soboleva, Pavel S. Dmitrenok, Sergey E Sedykh, Georgy A NevinskyAbstract:Human Placenta is an organ which protects, feeds, and regulates the grooving of the embryo. Therefore, identification and characterization of Placental components including proteins and their multi-protein complexes is an important step to understanding the Placenta function. We have obtained and analyzed for the first time an extremely stable multi-protein complex (SPC, ~1000 kDa) from the soluble fraction of three human Placentas. By gel filtration on Sepharose-4B, the SPC was well separated from other proteins of the Placenta Extract. Light scattering measurements and gel filtration showed that the SPC is stable in the presence of NaCl, MgCl2, acetonitrile, guanidinium chloride, and Triton in high concentrations, but dissociates efficiently in the presence of 8 M urea, 50 mM EDTA, and 0.5 M NaCl. Such a stable complex is unlikely to be a casual associate of different proteins. According to SDS-PAGE and MALDI mass spectrometry data, this complex contains many major glycosylated proteins with low and moderate molecular masses (MMs) 4–14 kDa and several moderately abundant (79.3, 68.5, 52.8, and 27.2 kDa) as well as minor proteins with higher MMs. The SPC treatment with dithiothreitol led to a disappearance of some protein bands and revealed proteins with lower MMs. The SPCs from three Placentas efficiently hydrolyzed plasmid supercoiled DNA with comparable rates and possess at least two DNA-binding sites with different affinities for a 12-mer oligonucleotide. Progress in study of Placental protein complexes can promote understanding of their biological functions.
Insop Shim - One of the best experts on this subject based on the ideXlab platform.
-
Anti-stress effects of human Placenta Extract: possible involvement of the oxidative stress system in rats
BMC Complementary and Alternative Medicine, 2018Co-Authors: Hyun-jung Park, Hae Jung Han, Hyun Soo Shim, Sunyoung Lee, Dae Hyun Hahm, Hyejung Lee, Insop ShimAbstract:Background Human Placenta hydrolysate (hPH) has been utilized to improve menopausal, fatigue, liver function. Its high concentration of bioactive substances is known to produce including antioxidant, anti-inflammatory and anti-nociceptive activities. However, its mechanisms of stress-induced depression remain unknown. Methods The present study examined the effect of hPH on stress-induced depressive behaviors and biochemical parameters in rats. hPH (0.02 ml, 0.2 ml or 1 ml/rat) was injected intravenously 30 min before the daily stress session in male Sprague-Dawley rats exposed to repeated immobilization stress (4 h/day for 7 days). The depressive-like behaviors of all groups were measured by elevated plus maze (EPM) and forced swimming test (FST). After the behavior tests, brain samples of all groups were collected for the analysis of glutathione peroxidase (GPx) and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) staining. Results Treatment with hPH produced a significant decrease of immobility time in the FST compared to the controls. Additionally, hPH treatment elicited a slightly decreasing trend in anxiety behavior on the EPM. Furthermore, hPH increased the level of GPx protein in the hippocampus, and decreased the expression of NADPH-d in the paraventricular nucleus (PVN). Conclusion This study demonstrated that hPH has anti-stress effects via the regulation of nitric oxide (NO) synthase and antioxidant activity in the brain. These results suggest that hPH may be useful in the treatment of stress-related diseases such as chronic fatigue syndrome.
-
Anti-stress effects of human Placenta Extract: possible involvement of the oxidative stress system in rats.
BMC Complementary and Alternative Medicine, 2018Co-Authors: Hyun-jung Park, Hae Jung Han, Hyun Soo Shim, Sunyoung Lee, Dae Hyun Hahm, Hyejung Lee, Insop ShimAbstract:Human Placenta hydrolysate (hPH) has been utilized to improve menopausal, fatigue, liver function. Its high concentration of bioactive substances is known to produce including antioxidant, anti-inflammatory and anti-nociceptive activities. However, its mechanisms of stress-induced depression remain unknown. The present study examined the effect of hPH on stress-induced depressive behaviors and biochemical parameters in rats. hPH (0.02 ml, 0.2 ml or 1 ml/rat) was injected intravenously 30 min before the daily stress session in male Sprague-Dawley rats exposed to repeated immobilization stress (4 h/day for 7 days). The depressive-like behaviors of all groups were measured by elevated plus maze (EPM) and forced swimming test (FST). After the behavior tests, brain samples of all groups were collected for the analysis of glutathione peroxidase (GPx) and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) staining. Treatment with hPH produced a significant decrease of immobility time in the FST compared to the controls. Additionally, hPH treatment elicited a slightly decreasing trend in anxiety behavior on the EPM. Furthermore, hPH increased the level of GPx protein in the hippocampus, and decreased the expression of NADPH-d in the paraventricular nucleus (PVN). This study demonstrated that hPH has anti-stress effects via the regulation of nitric oxide (NO) synthase and antioxidant activity in the brain. These results suggest that hPH may be useful in the treatment of stress-related diseases such as chronic fatigue syndrome.
Harald Tschesche - One of the best experts on this subject based on the ideXlab platform.
-
Proteases and their inhibitors are indicative in gestational disease
European Journal of Obstetrics & Gynecology and Reproductive Biology, 1996Co-Authors: M Kolben, A. Lopens, J Blaser, K. Ulm, Manfred Schmitt, Ktm Schneider, Harald TschescheAbstract:Objective: To assess whether various proteolytic factors which are involved in trophoblast invasion show different concentrations in plasma and Placenta of patients with HELLP syndrome, pre-/eclampsia and highly pathological Doppler flow measurements but without additional complications (hpD). Design: Case control and observational study; 18 women with HELLP syndrome, 21 with pre-/eclampsia, 13 with hpD, as well as healthy pregnant women (matched pairs); statistical analysis: sign test and Wilcoxon test. Results: Urokinase-type plasminogen activator (uPA), uPA receptor, tissue-type plasminogen activator (tPA), plasminogen activator inhibitor 1 (PAI-1), matrix metalloproteinases MMP-8, MMP-9 and tissue inhibitor of metalloproteinases TIMP-1 were measured by ELISA. PAI-1 plasma levels are significantly elevated in all three groups studied. In HELLP syndrome, tPA and TIMP-1 are also elevated, and in patients with hpD, MMP-8 is increased, whereas MMP-9, and TIMP-1 are lower. In Placenta Extract, only pre-/eclampsia shows reduced MMP-9 concentrations. Conclusions: The increased frequency of small-for-gestational-age infants observed in all three study groups is an expression of impaired Placental implantation and remodelling processes. These disturbances manifest themselves in the form of changes in some of the factors in plasma and Placenta Extract that are involved in these processes.
-
Concentration of different proteolytic and angiogenic factors in plasma and Placenta Extract of patients with HELLP syndrome, pre-/eclampsia, and highly pathological Doppler flow measurements
1995Co-Authors: M Kolben, A. Lopens, J Blaser, K. Ulm, Ktm Schneider, A. Huber, M. Frank, Olaf Wilhelm, S. Wilhelm, Harald TschescheAbstract:Objective: Impaired trophoblast invasion plays a major role in the development of preeclampsia. Therefore various factors that are involved in invasion were investigated in gestational disease. Methods: In pregnant women with HELLP-syndrome (n = 18), pre-/eclampsia (n = 21) and highly pathological Doppler flow measurements (hpD) (n = 13), plasma and Placental tissue Extract concentrations of uPA, uPA-receptor, tPA, PAI-1, MMP-8, MMP-9, TIMP-1, thrombomodulin, and angiogenin were measured using ELISA. Results: In all three collectives, PAI-1 plasma concentrations were significantly higher (p < 0,05) than in normal pregnancies, in patients with HELLP-syndrome, tPA and TIMP-1 plasma levels were also elevated. MMP-9 concentrations in Placental tissue Extracts were lower in pre-/eclampsia than in normal pregnancies. Conclusions: Impaired Placental implantation and remodelling in gestational disease is reflected by changes in plasma and Placental tissue Extract concentrations of various factors that are involved in these processes.
-
measuring the concentration of various plasma and Placenta Extract proteolytic and vascular factors in pregnant patients with hellp syndrome pre eclampsia and highly pathologic doppler flow values
Gynakologisch-geburtshilfliche Rundschau, 1995Co-Authors: M Kolben, A. Lopens, J Blaser, K. Ulm, Ktm Schneider, A. Huber, M. Frank, Olaf Wilhelm, S. Wilhelm, Harald TschescheAbstract:OBJECTIVE Impaired trophoblast invasion plays a major role in the development of preeclampsia. Therefore various factors that are involved in invasion were investigated in gestational disease. METHODS In pregnant women with HELLP-syndrome (n = 18), pre-/eclampsia (n = 21) and highly pathological Doppler flow measurements (hpD) (n = 13), plasma and Placental tissue Extract concentrations of uPA, uPA-receptor, tPA, PAI-1, MMP-8, MMP-9, TIMP-1, thrombomodulin, and angiogenin were measured using ELISA. RESULTS In all three collectives, PAI-1 plasma concentrations were significantly higher (p < 0,05) than in normal pregnancies, in patients with HELLP-syndrome, tPA and TIMP-1 plasma levels were also elevated. MMP-9 concentrations in Placental tissue Extracts were lower in pre-/eclampsia than in normal pregnancies. CONCLUSIONS Impaired Placental implantation and remodelling in gestational disease is reflected by changes in plasma and Placental tissue Extract concentrations of various factors that are involved in these processes.
Hae Jung Han - One of the best experts on this subject based on the ideXlab platform.
-
Effects of Human Placenta Extract (Laennec) on Ligament Healing in a Rodent Model.
Biological & Pharmaceutical Bulletin, 2019Co-Authors: Eun Ho Shin, Moonhang Kim, Binika Hada, Min Jung Jang, Jeom Yong Kim, Hae Jung Han, Dong Hwan Kim, Byung Hyune Choi, Bom Soo KimAbstract:Rich in bioactive substances such as amino acids and peptides, Laennec (human Placenta hydrolysate) has been widely used to control various types of musculoskeletal pain. However, the effects of Laennec on tendon and ligament injuries are not clearly understood. In the present study, Laennec was tested to identify its in vivo effects on ligament injury in an animal model and its in vitro effects on tendon-derived fibrocytes. A total of 99 Sprague Dawley rats were divided into the negative control (normal) group (n = 11) and the ligament injury group (n = 88). The ligament injury group was subdivided into normal saline-treated group, Laennec-treated group, polydeoxyribonucleotide-treated group, and 20% dextrose-treated group. Ligaments were collected at 1 week and 4 weeks after treatment. Histologic and biomechanical properties were analyzed. In vitro effects of Laennec and polydeoxyribonucleotide on fibrocytes were also analyzed. Although all other treatment groups showed increased inflammatory cells, the Laennec-treated group maintained cell counts and activated macrophage levels that were similar to the normal group. Unlike the saline-treated group and dextrose-treated group, the Laennec-treated group had low levels of degenerative changes at 4 weeks after treatment. Supportively, in vitro results showed that the Laennec-treated group had increased collagen type I, scleraxis (Scx) and tenomodulin (Tnmd) expression (p
-
effects of human Placenta Extract laennec on ligament healing in a rodent model
Biological & Pharmaceutical Bulletin, 2019Co-Authors: Eun Ho Shin, Moonhang Kim, Binika Hada, Min Jung Jang, Jeom Yong Kim, Hae Jung Han, Dong Hwan Kim, Byung Hyune Choi, Bom Soo KimAbstract:Rich in bioactive substances such as amino acids and peptides, Laennec (human Placenta hydrolysate) has been widely used to control various types of musculoskeletal pain. However, the effects of Laennec on tendon and ligament injuries are not clearly understood. In the present study, Laennec was tested to identify its in vivo effects on ligament injury in an animal model and its in vitro effects on tendon-derived fibrocytes. A total of 99 Sprague Dawley rats were divided into the negative control (normal) group (n = 11) and the ligament injury group (n = 88). The ligament injury group was subdivided into normal saline-treated group, Laennec-treated group, polydeoxyribonucleotide-treated group, and 20% dextrose-treated group. Ligaments were collected at 1 week and 4 weeks after treatment. Histologic and biomechanical properties were analyzed. In vitro effects of Laennec and polydeoxyribonucleotide on fibrocytes were also analyzed. Although all other treatment groups showed increased inflammatory cells, the Laennec-treated group maintained cell counts and activated macrophage levels that were similar to the normal group. Unlike the saline-treated group and dextrose-treated group, the Laennec-treated group had low levels of degenerative changes at 4 weeks after treatment. Supportively, in vitro results showed that the Laennec-treated group had increased collagen type I, scleraxis (Scx) and tenomodulin (Tnmd) expression (p < 0.05). Our study demonstrates that Laennec treatment enhances wound healing of damaged ligament by suppressing immune responses and reducing degenerative changes of damaged ligament. In addition, we found that Laennec induces the gene expression of type I collagen, Scx and Tnmd in fibrocytes, suggesting that Laennec may facilitate regeneration of damaged ligaments. Therefore, we expect that Laennec can be a useful drug to treat injured ligament.
-
Anti-stress effects of human Placenta Extract: possible involvement of the oxidative stress system in rats
BMC Complementary and Alternative Medicine, 2018Co-Authors: Hyun-jung Park, Hae Jung Han, Hyun Soo Shim, Sunyoung Lee, Dae Hyun Hahm, Hyejung Lee, Insop ShimAbstract:Background Human Placenta hydrolysate (hPH) has been utilized to improve menopausal, fatigue, liver function. Its high concentration of bioactive substances is known to produce including antioxidant, anti-inflammatory and anti-nociceptive activities. However, its mechanisms of stress-induced depression remain unknown. Methods The present study examined the effect of hPH on stress-induced depressive behaviors and biochemical parameters in rats. hPH (0.02 ml, 0.2 ml or 1 ml/rat) was injected intravenously 30 min before the daily stress session in male Sprague-Dawley rats exposed to repeated immobilization stress (4 h/day for 7 days). The depressive-like behaviors of all groups were measured by elevated plus maze (EPM) and forced swimming test (FST). After the behavior tests, brain samples of all groups were collected for the analysis of glutathione peroxidase (GPx) and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) staining. Results Treatment with hPH produced a significant decrease of immobility time in the FST compared to the controls. Additionally, hPH treatment elicited a slightly decreasing trend in anxiety behavior on the EPM. Furthermore, hPH increased the level of GPx protein in the hippocampus, and decreased the expression of NADPH-d in the paraventricular nucleus (PVN). Conclusion This study demonstrated that hPH has anti-stress effects via the regulation of nitric oxide (NO) synthase and antioxidant activity in the brain. These results suggest that hPH may be useful in the treatment of stress-related diseases such as chronic fatigue syndrome.
-
Anti-stress effects of human Placenta Extract: possible involvement of the oxidative stress system in rats.
BMC Complementary and Alternative Medicine, 2018Co-Authors: Hyun-jung Park, Hae Jung Han, Hyun Soo Shim, Sunyoung Lee, Dae Hyun Hahm, Hyejung Lee, Insop ShimAbstract:Human Placenta hydrolysate (hPH) has been utilized to improve menopausal, fatigue, liver function. Its high concentration of bioactive substances is known to produce including antioxidant, anti-inflammatory and anti-nociceptive activities. However, its mechanisms of stress-induced depression remain unknown. The present study examined the effect of hPH on stress-induced depressive behaviors and biochemical parameters in rats. hPH (0.02 ml, 0.2 ml or 1 ml/rat) was injected intravenously 30 min before the daily stress session in male Sprague-Dawley rats exposed to repeated immobilization stress (4 h/day for 7 days). The depressive-like behaviors of all groups were measured by elevated plus maze (EPM) and forced swimming test (FST). After the behavior tests, brain samples of all groups were collected for the analysis of glutathione peroxidase (GPx) and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) staining. Treatment with hPH produced a significant decrease of immobility time in the FST compared to the controls. Additionally, hPH treatment elicited a slightly decreasing trend in anxiety behavior on the EPM. Furthermore, hPH increased the level of GPx protein in the hippocampus, and decreased the expression of NADPH-d in the paraventricular nucleus (PVN). This study demonstrated that hPH has anti-stress effects via the regulation of nitric oxide (NO) synthase and antioxidant activity in the brain. These results suggest that hPH may be useful in the treatment of stress-related diseases such as chronic fatigue syndrome.