The Experts below are selected from a list of 261 Experts worldwide ranked by ideXlab platform
N Chaffey - One of the best experts on this subject based on the ideXlab platform.
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Plant Cuttings, December
2012Co-Authors: N ChaffeyAbstract:Nigel Chaffey presents a round-up of Plant-based items from the world's media. Articles include: Problems with models? / Pollia pigmentation pooh-poohed / A bit of warmth and we all go bananas!? / Duke botanists go gaga over ferns / Turing's centenial flowers.
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Plant Cuttings, August
2012Co-Authors: N ChaffeyAbstract:Nigel Chaffey presents a round-up of Plant-based items from the world's media. Articles include: Bigging-up the AoB Blog [or, Cann can – and does!] / Suck on this: predictions and patience in phytobiology / Rejoice, ‘tis the real Bloom's Day (to be sure) / Sex inequality in science: skirting around the issue? / Botany? It's all about app-lication … / Lichens, thale cress, tobacco, the great survivors …
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Plant Cuttings, February
2012Co-Authors: N ChaffeyAbstract:Nigel Chaffey presents a round-up of Plant-based items from the world's media. Articles include: Plant parts doing unexpected things: Part 1 (or, Giving transpiration a little boost …) / 2015 International Year of … / Plant parts doing unexpected things: Part 2 (or, Root research all up in the air[1]) / The great botanical countdown / References.
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Plant Cuttings, June
2011Co-Authors: N ChaffeyAbstract:Nigel Chaffey presents a round-up of Plant-based items from the world's media. Articles include: It's not what you know, but who you know! / Leeuwenhoek whine a stinker … / Back to the Beginning: The Miller's Tale / Where have all the Plants come from? / The ultimate packed lunch? / Hay fever: another straw to clutch at? / Genome jealousy / Say it with … fungi?
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Plant Cuttings, May
2011Co-Authors: N ChaffeyAbstract:Nigel Chaffey presents a round-up of Plant-based items from the world's media. Articles include: Roles for calcium: numero uno(!) / Has spring sprung yet? / Gum's the word / An over-abundance of nitrogen …? / The positives of parasitic Plants(!) / Blessed are the peas-makers …
Jeanfrancois Ferard - One of the best experts on this subject based on the ideXlab platform.
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assessment of the genotoxicity of contaminated soil with the allium vicia micronucleus and the tradescantia micronucleus assays
Mutation Research, 1999Co-Authors: Sylvie Cotelle, Jeanfrancois Masfaraud, Jeanfrancois FerardAbstract:The present study concerns the genotoxicity of contaminated soil near Metz, France. Three Plant bioassays, the Vicia faba (broad bean), the Allium cepa (white onion) and the Tradescantia (spiderwort) micronuclei tests were used to evaluate for genotoxicity. Two soil samples were tested: soil sample A (from an industrial waste site) and soil sample B (from a cokeworks waste site). Maleic hydrazide was used as the positive control. Aqueous extracts of the soil samples were used to treat the roots of Vicia and Allium, and Plant Cuttings of Tradescantia according to the standard protocol for these Plant assays established by the International Program on Chemical Safety and the World Health Organization. The results of these tests showed differential sensitivity in the three different bioassays. Soil sample A was more toxic than soil sample B.
Xunling Wang - One of the best experts on this subject based on the ideXlab platform.
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The Tradescantia-micronucleus test on the genotoxicity of UV-B radiation
Mutation research, 1999Co-Authors: Shuyu Wang, Xunling WangAbstract:Abstract Lanzhou city is located in north central China near inner Mongolia. The solar UV-B background radiation in this area is occasionally extremely high (8 μW/cm2). Such high background solar UV-B radiation could be attributed to the sporadic depletion of the ozone layer in the stratosphere. The excessive UV-B radiation is a potential hazard in the environment. This prompted the present study on the effect of UV-B radiation on the cytogenetic damage to pollen mother cells of the Plant Tradescantia. The Tradescantia-micronucleus (Trad-MCN) test was used to determine the genotoxicity of UV-B radiation. In addition to the usual 10 h of solar emission of UV light a series of increasing dosages (2, 4, 6, 8 h) of artificial UV-B radiation was applied to Tradescantia (clone 3) Plant Cuttings. Inflorescences of the treated and control Plants were fixed and used for preparation of microslides. Micronuclei frequencies were observed in the early tetrads to show the degree of genotoxicity. Results of two repeated experiments show a dose-related increase of MCN frequencies under normal sunshine days. In the third experiment conducted under a cloudy and rainy day and an extraordinary high solar UV-B background, the MCN frequencies were markedly higher than that of the negative control but did not show the clear dose response to the treatment as in the first two experiments. The Trad-MCN test has successfully detected the effect of artificial UV-B radiation over the solar UV-B background radiation.
M F Salamone - One of the best experts on this subject based on the ideXlab platform.
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the use of tradescantia and vicia faba bioassays for the in situ detection of mutagens in an aquatic environment
Mutation Research, 1992Co-Authors: William F Gra, H G Lee, D M Loga, M F SalamoneAbstract:Tests have shown Plant bioassays to be excellent for mutagenicity studies. Most studies with Plant bioassays, however, have been carried out either in the laboratory, or if, in situ, as monitors of atmospheric contaminants. The primary purpose of this study was to assess the utility of in situ Plant mutagenicity bioassays in monitoring water contaminants. The assay systems tested were the Tradescantia stamen hair and micronucleus assays for the detection of gene mutations and chromosomal aberrations respectively, and the Vicia faba bioassay system which detects chromosomal aberrations in root tips. The assays were used to test the effluent from a pulp and paper mill located on the north shore of Lake Superior. Assays were performed in a creek containing raw effluent and in the bay of Lake Superior into which the creek emptied. All in situ treatments were carried out for 24 h. The effluent from the creek was heavy with pulp and debris which coated the Plant Cuttings and the Vicia faba seedlings and may have restricted the uptake from the effluent. In the creek, at test sites 11.5 km from the source, the effluent was toxic to the Vicia faba roots as evidenced by a reduction in the mitotic index. The data for the Tradescantia stamen hair assay in the creek were equivocal. The Cuttings from the creek test sites and the air and water control sites appeared to have undergone a physiological delay. Within a day or two after the return to the laboratory, that is 6-8 days after testing, flowering almost ceased and did not fully resume until about day 35. This reduction in flowering was particularly severe with the Cuttings from the effluent and air control sites, making it very difficult to interpret the results. In contrast, the Tradescantia micronucleus and Vicia faba chromosomal aberration data were unequivocal; each produced positive responses at both test sites relative to the air and water controls. The results obtained for the bay sites with all 3 assays were in agreement. In that section of the bay visibly contaminated by the creek effluent, increases in stamen hair mutants, micronuclei, and chromosome aberrations were measured. In general, there was a considerable reduction in the number of mutant events observed for the water samples brought back from the test sites and tested in the laboratory.(ABSTRACT TRUNCATED AT 400 WORDS)
Olga A. Aleynova - One of the best experts on this subject based on the ideXlab platform.
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The calcium-dependent protein kinase gene VaCPK29 is involved in grapevine responses to heat and osmotic stresses
Plant Growth Regulation, 2017Co-Authors: Alexandra S. Dubrovina, Konstantin V. Kiselev, Valeriya S. Khristenko, Olga A. AleynovaAbstract:Ca^2+-dependent protein kinases (CDPKs or CPKs) are essential primary sensors of Ca^2+ in Plants and are known to play important roles in Plant abiotic and biotic stress responses. Vitis amurensis is a wild grapevine species with a high level of cold and disease resistance. It has previously been shown that transcription of 10 CDPK genes of V. amurensis was elevated under salt, desiccation, high mannitol, cold, and heat stress conditions. Expression of VaCPK29 was induced under high and low temperatures, water deficit, and high mannitol stress in Plant Cuttings of V. amurensis . The present study revealed that the callus cell cultures of V. amurensis and soil-grown Plants of Arabidopsis thaliana overexpressing VaCPK29 exhibited higher tolerance to heat and high mannitol stress in comparison with the control transformed with the empty vector. Cold, salt, and drought stress tolerance of the transgenic V. amurensis calli and A. thaliana Plants was comparable to that of the controls. The stress-responsive genes AtDREB1A, AtDREB2A, AtRD29A, AtRD29B , and AtABF3 were up-regulated in the VaCPK29 -overexpressing A. thaliana Plants under heat stress. Taken together, the data indicate that the VaCPK29 gene may act as a positive regulator in the grapevine response to heat and osmotic stresses.
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vacpk21 a calcium dependent protein kinase gene of wild grapevine vitis amurensis rupr is involved in grape response to salt stress
Plant Cell Tissue and Organ Culture, 2016Co-Authors: Alexandra S. Dubrovina, Konstantin V. Kiselev, Valeriya S. Khristenko, Olga A. AleynovaAbstract:Ca2+-dependent protein kinases (CDPKs) are known to play important roles in signal transduction in response to various abiotic stresses that limit crop yields. Vitis amurensis is a wild grape species that possesses a high adaptive potential and displays a high resistance to environmental stresses, such as low temperatures or microbial pathogens. The roles of most grape CDPKs in the adaptation to various stresses have not yet been uncovered. A recent CDPK gene expression analysis revealed that expression of VaCPK21 gene was significantly up-regulated under salt stress and, to a less degree, under high mannitol and high temperature stresses in V. amurensis Plant Cuttings. In this study, the effects of overexpressing the VaCPK21 gene in callus cell lines of V. amurensis and transgenic Plants of A. thaliana on their responses to abiotic stresses were investigated. The VaCPK21-overexpressing cell cultures of V. amurensis, soil-grown Plants of A. thaliana, and seedlings of A. thaliana exhibited higher tolerance to salt stress in comparison with the controls transformed with the “empty” vector. Heat, cold, and drought stress resistance of the transgenic V. amurensis calli and A. thaliana Plants was comparable to that of the controls. Under salt stress conditions, the stress-related genes AtCOR15, AtCOR47, AtCAT1, AtCSD1, AtNHX1, AtKIN1, AtRD26, and AtRD29B demonstrated strong up-regulation in the VaCPK21-transgenic but not in the control Arabidopsis Plants. Together, the results indicate that VaCPK21 may act as a positive regulator involved in the grapevine response to salt stress.