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Woosik Jeong - One of the best experts on this subject based on the ideXlab platform.
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antioxidant and hepatoprotective effects of procyanidins from Wild Grape vitis amurensis seeds in ethanol induced cells and rats
International Journal of Molecular Sciences, 2016Co-Authors: Minji Bak, Mira Jun, Van Long Truong, Xuan Ngan Giang Nguyen, Pajaree Ingkasupart, Jin Young Shin, Woosik JeongAbstract:In the present study, we characterized the antioxidant and hepatoprotective mechanisms underlying of Wild Grape seed procyanidins (WGP) against oxidative stress damage in ethanol-treated HepG2 cell and Sprague-Dawley (SD)-rat models. In HepG2 cells, WGP not only diminished the ethanol (EtOH, 100 mM)-induced reactive oxygen species (ROS) formation and cytochrome P450 2E1 (CYP2E1) expression, but also renovated both the activity and expression of antioxidant enzymes including catalase, superoxide dismutase, and glutathione peroxidase. Additionally, to investigate the hepatoprotective effect of WGP, rats were orally administered 10 or 50 mg/kg WGP once daily for seven days prior to the single oral administration of EtOH (6 g/kg). The results show that WGP administration decreased the EtOH-induced augment of the levels of serum aspartate transaminase and alanine transaminase as well as serum alcohol and acetaldehyde. WGP treatment upregulated the activities and protein levels of hepatic alcohol dehydrogenase, aldehyde dehydrogenase, and antioxidant enzymes but downregulated the protein expression level of liver CYP2E1 in EtOH-treated rats. Moreover, the decreased phosphorylation levels of mitogen activated protein kinases (MAPKs) by ethanol were induced in both HepG2 cell and rat models. Overall, pretreatment of WGP displayed the protective activity against EtOH-mediated toxicity through the regulation of antioxidant enzymes and alcohol metabolism systems via MAPKs pathways.
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anti inflammatory effect of procyanidins from Wild Grape vitis amurensis seeds in lps induced raw 264 7 cells
Oxidative Medicine and Cellular Longevity, 2013Co-Authors: Minji Bak, Mira Jun, Van Long Truong, Heysook Kang, Woosik JeongAbstract:In the present study, the anti-inflammatory effect and underlying mechanisms of Wild Grape seeds procyanidins (WGP) were examined using lipopolysaccharide- (LPS-) stimulated RAW 264.7 cells. We used nitric oxide (NO) and prostaglandin E2 (PGE2) and reactive oxygen species (ROS) assays to examine inhibitory effect of WGP and further investigated the mechanisms of WGP suppressed LPS-mediated genes and upstream expression by Western blot and confocal microscopy analysis. Our data indicate that WGP significantly reduced NO, PGE2, and ROS production and also inhibited the expression of proinflammatory mediators such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expressions. Consistently, WGP significantly reduced LPS-stimulated expression of proinflammatory cytokines such as tumor necrosis factor α (TNF-α) and interleukin- (IL-) 1β. Moreover, WGP prevented nuclear translocation of nuclear factor-κB (NFκB) p65 subunit by reducing inhibitory κB-α (IκBα) and NFκB phosphorylation. Furthermore, we found that WGP inhibited LPS-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK). Taken together, our results demonstrated that WGP exerts potent anti-inflammatory activity through the inhibition of iNOS and COX-2 by regulating NFκB and p38 MAPK pathway.
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procyanidins from Wild Grape vitis amurensis seeds regulate are mediated enzyme expression via nrf2 coupled with p38 and pi3k akt pathway in hepg2 cells
International Journal of Molecular Sciences, 2012Co-Authors: Minji Bak, Mira Jun, Woosik JeongAbstract:Procyanidins, polymers of flavan-3-ol units, have been reported to exhibit many beneficial health effects such as antioxidant and anti-carcinogenic effects. In this study, we investigated the cancer chemopreventive properties of procyanidins from Wild Grape (Vitis amurensis) seeds in particular their roles in inducing phase II detoxifying/antioxidant enzymes as well as in modulating the upstream kinases. Ethanolic extract of V. amurensis seeds was fractionated with a series of organic solvents and finally separated into six fractions, F1–F6. Chemical properties of the procyanidins were analyzed by vanillin assay, BuOH-HCl test, and depolymerization with phloroglucinol followed by LC/MS analysis. The F5 had the highest procyanidin content among all the fractions and strongly induced the reporter activity of antioxidant response element as well as the protein expression of nuclear factor E2-related factor (Nrf2) in HepG2 human hepatocarcinoma cells. The procyanidin-rich F5 also strongly induced the expression of the phase II detoxifying and antioxidant enzymes such as NAD(P)H:quinone oxidoreductase1 and hemeoxygenase1. Phosphorylations of the upstream kinases such as MAPKs and PI3K/Akt were significantly increased by treatment with procyanidin fraction. In addition, the procyanidin-mediated Nrf2 expression was partly attenuated by PI3K inhibitor LY294002, and almost completely by p38 inhibitor SB202190, but neither by JNK inhibitor SP600125 nor by MEK1/2 inhibitor U0126. Taken together, the procyanidins from Wild Grape seeds could be used as a potential natural chemopreventive agent through Nrf2/ARE-mediated phase II detoxifying/antioxidant enzymes induction via p38 and PI3K/Akt pathway.
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antioxidant properties of proanthocyanidin fraction isolated from Wild Grape vitis amurensis peel
Korean Journal of Food Science and Technology, 2010Co-Authors: Hye-ryun Lee, Woosik Jeong, Young-chan Kim, Inwook Hwang, Huzhe Zheng, Shin-kyo ChungAbstract:The proanthocyanidin fraction was isolated from the Wild Grape (Vitis amurensis) peel and its antioxidant capacities were examined to promote the utilization of Wild Grape by-products. The 70% acetone crude extract of the Wild Grape peel was fractionated with hexane, ethyl acetate, and water. The ethyl acetate fraction was applied to a Sephadex LH-20 column chromatograph, which was eluted with 50% methanol, 75% methanol, and 75% acetone. The proanthocyanidin characteristics and contents of the isolated fractions were investigated by the vanillin- and BuOH-HCl methods. Fraction 6 had the highest proanthocyanidin content () among the isolated fractions. The antioxidant activities of the proanthocyanidin fraction were examined by DPPH radical scavenging, FRAP assay, and total phenolic contents. The FRAP values and total phenolic contents of the fractions ranged from 3.54 to 32.25 mmol/kg and from 4.48 to 50.80 g/100 g, respectively. The proanthocyanidin contents was strongly correlated with DPPH radical scavenging activities, FRAP values, and total phenolic contents.
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Antioxidant properties of proanthocyanidin fraction isolated from Wild Grape (Vitis amurensis) seed
Journal of the Korean Society for Applied Biological Chemistry, 2009Co-Authors: Hye-ryun Lee, Minji Bak, Woosik Jeong, Young-chan Kim, Shin-kyo ChungAbstract:In order to investigate the utilization of Wild Grape by-products, isolation of proanthocyanidin from the Wild Grape seed (Vitis amurensis) and its antioxidant capacities were examined. 70% acetone crude extract of the Wild Grape seed was fractionated. The ethyl acetate fraction was applied to Sephadex LH-20 column chromatography, which was eluted with 50%, 75% methanol, and 75% acetone, respectively, and 9 fractions were collected, finally. Their proanthocyanidin characteristics and contents were investigated by vanillin-H2SO4 and BuOH-HCl methods. The Fr. 5 has the highest proanthocyanidin content (86.00±6.74 g%) among all the fractions. Fr. 6-2 was identified as (+)-catechin by LC-MS analysis. The antioxidant activities of the proanthocyanidin were evaluated by total phenolic contents, DPPH radical scavenging, and FRAP assays. Fr. 8 shows the strongest antioxidant activities with high proanthocyanidin content. The Wild Grape seed containing fairly high amounts of proanthocyanidin could be utilized as a natural antioxidant material.
Minji Bak - One of the best experts on this subject based on the ideXlab platform.
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antioxidant and hepatoprotective effects of procyanidins from Wild Grape vitis amurensis seeds in ethanol induced cells and rats
International Journal of Molecular Sciences, 2016Co-Authors: Minji Bak, Mira Jun, Van Long Truong, Xuan Ngan Giang Nguyen, Pajaree Ingkasupart, Jin Young Shin, Woosik JeongAbstract:In the present study, we characterized the antioxidant and hepatoprotective mechanisms underlying of Wild Grape seed procyanidins (WGP) against oxidative stress damage in ethanol-treated HepG2 cell and Sprague-Dawley (SD)-rat models. In HepG2 cells, WGP not only diminished the ethanol (EtOH, 100 mM)-induced reactive oxygen species (ROS) formation and cytochrome P450 2E1 (CYP2E1) expression, but also renovated both the activity and expression of antioxidant enzymes including catalase, superoxide dismutase, and glutathione peroxidase. Additionally, to investigate the hepatoprotective effect of WGP, rats were orally administered 10 or 50 mg/kg WGP once daily for seven days prior to the single oral administration of EtOH (6 g/kg). The results show that WGP administration decreased the EtOH-induced augment of the levels of serum aspartate transaminase and alanine transaminase as well as serum alcohol and acetaldehyde. WGP treatment upregulated the activities and protein levels of hepatic alcohol dehydrogenase, aldehyde dehydrogenase, and antioxidant enzymes but downregulated the protein expression level of liver CYP2E1 in EtOH-treated rats. Moreover, the decreased phosphorylation levels of mitogen activated protein kinases (MAPKs) by ethanol were induced in both HepG2 cell and rat models. Overall, pretreatment of WGP displayed the protective activity against EtOH-mediated toxicity through the regulation of antioxidant enzymes and alcohol metabolism systems via MAPKs pathways.
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anti inflammatory effect of procyanidins from Wild Grape vitis amurensis seeds in lps induced raw 264 7 cells
Oxidative Medicine and Cellular Longevity, 2013Co-Authors: Minji Bak, Mira Jun, Van Long Truong, Heysook Kang, Woosik JeongAbstract:In the present study, the anti-inflammatory effect and underlying mechanisms of Wild Grape seeds procyanidins (WGP) were examined using lipopolysaccharide- (LPS-) stimulated RAW 264.7 cells. We used nitric oxide (NO) and prostaglandin E2 (PGE2) and reactive oxygen species (ROS) assays to examine inhibitory effect of WGP and further investigated the mechanisms of WGP suppressed LPS-mediated genes and upstream expression by Western blot and confocal microscopy analysis. Our data indicate that WGP significantly reduced NO, PGE2, and ROS production and also inhibited the expression of proinflammatory mediators such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expressions. Consistently, WGP significantly reduced LPS-stimulated expression of proinflammatory cytokines such as tumor necrosis factor α (TNF-α) and interleukin- (IL-) 1β. Moreover, WGP prevented nuclear translocation of nuclear factor-κB (NFκB) p65 subunit by reducing inhibitory κB-α (IκBα) and NFκB phosphorylation. Furthermore, we found that WGP inhibited LPS-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK). Taken together, our results demonstrated that WGP exerts potent anti-inflammatory activity through the inhibition of iNOS and COX-2 by regulating NFκB and p38 MAPK pathway.
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procyanidins from Wild Grape vitis amurensis seeds regulate are mediated enzyme expression via nrf2 coupled with p38 and pi3k akt pathway in hepg2 cells
International Journal of Molecular Sciences, 2012Co-Authors: Minji Bak, Mira Jun, Woosik JeongAbstract:Procyanidins, polymers of flavan-3-ol units, have been reported to exhibit many beneficial health effects such as antioxidant and anti-carcinogenic effects. In this study, we investigated the cancer chemopreventive properties of procyanidins from Wild Grape (Vitis amurensis) seeds in particular their roles in inducing phase II detoxifying/antioxidant enzymes as well as in modulating the upstream kinases. Ethanolic extract of V. amurensis seeds was fractionated with a series of organic solvents and finally separated into six fractions, F1–F6. Chemical properties of the procyanidins were analyzed by vanillin assay, BuOH-HCl test, and depolymerization with phloroglucinol followed by LC/MS analysis. The F5 had the highest procyanidin content among all the fractions and strongly induced the reporter activity of antioxidant response element as well as the protein expression of nuclear factor E2-related factor (Nrf2) in HepG2 human hepatocarcinoma cells. The procyanidin-rich F5 also strongly induced the expression of the phase II detoxifying and antioxidant enzymes such as NAD(P)H:quinone oxidoreductase1 and hemeoxygenase1. Phosphorylations of the upstream kinases such as MAPKs and PI3K/Akt were significantly increased by treatment with procyanidin fraction. In addition, the procyanidin-mediated Nrf2 expression was partly attenuated by PI3K inhibitor LY294002, and almost completely by p38 inhibitor SB202190, but neither by JNK inhibitor SP600125 nor by MEK1/2 inhibitor U0126. Taken together, the procyanidins from Wild Grape seeds could be used as a potential natural chemopreventive agent through Nrf2/ARE-mediated phase II detoxifying/antioxidant enzymes induction via p38 and PI3K/Akt pathway.
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Antioxidant properties of proanthocyanidin fraction isolated from Wild Grape (Vitis amurensis) seed
Journal of the Korean Society for Applied Biological Chemistry, 2009Co-Authors: Hye-ryun Lee, Minji Bak, Woosik Jeong, Young-chan Kim, Shin-kyo ChungAbstract:In order to investigate the utilization of Wild Grape by-products, isolation of proanthocyanidin from the Wild Grape seed (Vitis amurensis) and its antioxidant capacities were examined. 70% acetone crude extract of the Wild Grape seed was fractionated. The ethyl acetate fraction was applied to Sephadex LH-20 column chromatography, which was eluted with 50%, 75% methanol, and 75% acetone, respectively, and 9 fractions were collected, finally. Their proanthocyanidin characteristics and contents were investigated by vanillin-H2SO4 and BuOH-HCl methods. The Fr. 5 has the highest proanthocyanidin content (86.00±6.74 g%) among all the fractions. Fr. 6-2 was identified as (+)-catechin by LC-MS analysis. The antioxidant activities of the proanthocyanidin were evaluated by total phenolic contents, DPPH radical scavenging, and FRAP assays. Fr. 8 shows the strongest antioxidant activities with high proanthocyanidin content. The Wild Grape seed containing fairly high amounts of proanthocyanidin could be utilized as a natural antioxidant material.
Mira Jun - One of the best experts on this subject based on the ideXlab platform.
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antioxidant and hepatoprotective effects of procyanidins from Wild Grape vitis amurensis seeds in ethanol induced cells and rats
International Journal of Molecular Sciences, 2016Co-Authors: Minji Bak, Mira Jun, Van Long Truong, Xuan Ngan Giang Nguyen, Pajaree Ingkasupart, Jin Young Shin, Woosik JeongAbstract:In the present study, we characterized the antioxidant and hepatoprotective mechanisms underlying of Wild Grape seed procyanidins (WGP) against oxidative stress damage in ethanol-treated HepG2 cell and Sprague-Dawley (SD)-rat models. In HepG2 cells, WGP not only diminished the ethanol (EtOH, 100 mM)-induced reactive oxygen species (ROS) formation and cytochrome P450 2E1 (CYP2E1) expression, but also renovated both the activity and expression of antioxidant enzymes including catalase, superoxide dismutase, and glutathione peroxidase. Additionally, to investigate the hepatoprotective effect of WGP, rats were orally administered 10 or 50 mg/kg WGP once daily for seven days prior to the single oral administration of EtOH (6 g/kg). The results show that WGP administration decreased the EtOH-induced augment of the levels of serum aspartate transaminase and alanine transaminase as well as serum alcohol and acetaldehyde. WGP treatment upregulated the activities and protein levels of hepatic alcohol dehydrogenase, aldehyde dehydrogenase, and antioxidant enzymes but downregulated the protein expression level of liver CYP2E1 in EtOH-treated rats. Moreover, the decreased phosphorylation levels of mitogen activated protein kinases (MAPKs) by ethanol were induced in both HepG2 cell and rat models. Overall, pretreatment of WGP displayed the protective activity against EtOH-mediated toxicity through the regulation of antioxidant enzymes and alcohol metabolism systems via MAPKs pathways.
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anti inflammatory effect of procyanidins from Wild Grape vitis amurensis seeds in lps induced raw 264 7 cells
Oxidative Medicine and Cellular Longevity, 2013Co-Authors: Minji Bak, Mira Jun, Van Long Truong, Heysook Kang, Woosik JeongAbstract:In the present study, the anti-inflammatory effect and underlying mechanisms of Wild Grape seeds procyanidins (WGP) were examined using lipopolysaccharide- (LPS-) stimulated RAW 264.7 cells. We used nitric oxide (NO) and prostaglandin E2 (PGE2) and reactive oxygen species (ROS) assays to examine inhibitory effect of WGP and further investigated the mechanisms of WGP suppressed LPS-mediated genes and upstream expression by Western blot and confocal microscopy analysis. Our data indicate that WGP significantly reduced NO, PGE2, and ROS production and also inhibited the expression of proinflammatory mediators such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expressions. Consistently, WGP significantly reduced LPS-stimulated expression of proinflammatory cytokines such as tumor necrosis factor α (TNF-α) and interleukin- (IL-) 1β. Moreover, WGP prevented nuclear translocation of nuclear factor-κB (NFκB) p65 subunit by reducing inhibitory κB-α (IκBα) and NFκB phosphorylation. Furthermore, we found that WGP inhibited LPS-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK). Taken together, our results demonstrated that WGP exerts potent anti-inflammatory activity through the inhibition of iNOS and COX-2 by regulating NFκB and p38 MAPK pathway.
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procyanidins from Wild Grape vitis amurensis seeds regulate are mediated enzyme expression via nrf2 coupled with p38 and pi3k akt pathway in hepg2 cells
International Journal of Molecular Sciences, 2012Co-Authors: Minji Bak, Mira Jun, Woosik JeongAbstract:Procyanidins, polymers of flavan-3-ol units, have been reported to exhibit many beneficial health effects such as antioxidant and anti-carcinogenic effects. In this study, we investigated the cancer chemopreventive properties of procyanidins from Wild Grape (Vitis amurensis) seeds in particular their roles in inducing phase II detoxifying/antioxidant enzymes as well as in modulating the upstream kinases. Ethanolic extract of V. amurensis seeds was fractionated with a series of organic solvents and finally separated into six fractions, F1–F6. Chemical properties of the procyanidins were analyzed by vanillin assay, BuOH-HCl test, and depolymerization with phloroglucinol followed by LC/MS analysis. The F5 had the highest procyanidin content among all the fractions and strongly induced the reporter activity of antioxidant response element as well as the protein expression of nuclear factor E2-related factor (Nrf2) in HepG2 human hepatocarcinoma cells. The procyanidin-rich F5 also strongly induced the expression of the phase II detoxifying and antioxidant enzymes such as NAD(P)H:quinone oxidoreductase1 and hemeoxygenase1. Phosphorylations of the upstream kinases such as MAPKs and PI3K/Akt were significantly increased by treatment with procyanidin fraction. In addition, the procyanidin-mediated Nrf2 expression was partly attenuated by PI3K inhibitor LY294002, and almost completely by p38 inhibitor SB202190, but neither by JNK inhibitor SP600125 nor by MEK1/2 inhibitor U0126. Taken together, the procyanidins from Wild Grape seeds could be used as a potential natural chemopreventive agent through Nrf2/ARE-mediated phase II detoxifying/antioxidant enzymes induction via p38 and PI3K/Akt pathway.
Van Long Truong - One of the best experts on this subject based on the ideXlab platform.
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antioxidant and hepatoprotective effects of procyanidins from Wild Grape vitis amurensis seeds in ethanol induced cells and rats
International Journal of Molecular Sciences, 2016Co-Authors: Minji Bak, Mira Jun, Van Long Truong, Xuan Ngan Giang Nguyen, Pajaree Ingkasupart, Jin Young Shin, Woosik JeongAbstract:In the present study, we characterized the antioxidant and hepatoprotective mechanisms underlying of Wild Grape seed procyanidins (WGP) against oxidative stress damage in ethanol-treated HepG2 cell and Sprague-Dawley (SD)-rat models. In HepG2 cells, WGP not only diminished the ethanol (EtOH, 100 mM)-induced reactive oxygen species (ROS) formation and cytochrome P450 2E1 (CYP2E1) expression, but also renovated both the activity and expression of antioxidant enzymes including catalase, superoxide dismutase, and glutathione peroxidase. Additionally, to investigate the hepatoprotective effect of WGP, rats were orally administered 10 or 50 mg/kg WGP once daily for seven days prior to the single oral administration of EtOH (6 g/kg). The results show that WGP administration decreased the EtOH-induced augment of the levels of serum aspartate transaminase and alanine transaminase as well as serum alcohol and acetaldehyde. WGP treatment upregulated the activities and protein levels of hepatic alcohol dehydrogenase, aldehyde dehydrogenase, and antioxidant enzymes but downregulated the protein expression level of liver CYP2E1 in EtOH-treated rats. Moreover, the decreased phosphorylation levels of mitogen activated protein kinases (MAPKs) by ethanol were induced in both HepG2 cell and rat models. Overall, pretreatment of WGP displayed the protective activity against EtOH-mediated toxicity through the regulation of antioxidant enzymes and alcohol metabolism systems via MAPKs pathways.
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anti inflammatory effect of procyanidins from Wild Grape vitis amurensis seeds in lps induced raw 264 7 cells
Oxidative Medicine and Cellular Longevity, 2013Co-Authors: Minji Bak, Mira Jun, Van Long Truong, Heysook Kang, Woosik JeongAbstract:In the present study, the anti-inflammatory effect and underlying mechanisms of Wild Grape seeds procyanidins (WGP) were examined using lipopolysaccharide- (LPS-) stimulated RAW 264.7 cells. We used nitric oxide (NO) and prostaglandin E2 (PGE2) and reactive oxygen species (ROS) assays to examine inhibitory effect of WGP and further investigated the mechanisms of WGP suppressed LPS-mediated genes and upstream expression by Western blot and confocal microscopy analysis. Our data indicate that WGP significantly reduced NO, PGE2, and ROS production and also inhibited the expression of proinflammatory mediators such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expressions. Consistently, WGP significantly reduced LPS-stimulated expression of proinflammatory cytokines such as tumor necrosis factor α (TNF-α) and interleukin- (IL-) 1β. Moreover, WGP prevented nuclear translocation of nuclear factor-κB (NFκB) p65 subunit by reducing inhibitory κB-α (IκBα) and NFκB phosphorylation. Furthermore, we found that WGP inhibited LPS-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK). Taken together, our results demonstrated that WGP exerts potent anti-inflammatory activity through the inhibition of iNOS and COX-2 by regulating NFκB and p38 MAPK pathway.
Chi-fai Chau - One of the best experts on this subject based on the ideXlab platform.
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Intestinal health benefits of the water-soluble carbohydrate concentrate of Wild Grape ( Vitis thunbergii ) in hamsters.
Journal of agricultural and food chemistry, 2012Co-Authors: Ya-ling Huang, Hui-fang Chu, Fan-jhen Dai, Chi-fai ChauAbstract:The dose–response relationship of the water-soluble carbohydrate concentrate (WSCC) from Wild Grape (Vitis thunbergii Sieb. & Zucc.) on intestinal health was investigated in this study. WSCC contained carbohydrates up to 71.9 g/100 g, including arabinose-rich pectic polysaccharide, hemicelluloses, glucose, and fructose. The consumption of WSCC (0.5 and 1.5 g/100 g of diet) effectively (P < 0.05) shortened gastrointestinal transit time (−62.3 to −63.0%), decreased toxic cecal ammonia (−59.3 to −63.0%) and daily fecal ammonia output (−29.7 to −41.4%), decreased the activities of fecal β-glucuronidase (−78.6%), β-glucosidase (−80.5 to −87.5%), mucinase (−64.6 to −72.7%), and urease (−83.2 to −86.0%), increased fecal moisture content (116–129%), and also increased short-chain fatty acid levels in cecal contents (1.8–3.3-fold). These findings suggested that consumption of Wild Grape WSCC might diminish the exposure of intestinal mucosa to toxic ammonia and other detrimental compounds and, hence exert, favorabl...
