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Thomas Roitsch - One of the best experts on this subject based on the ideXlab platform.

  • noninvasive phenotyping of Plant Pathogen Interaction consecutive in situ imaging of fluorescing pseudomonas syringae Plant phenolic fluorescence and chlorophyll fluorescence in arabidopsis leaves
    Frontiers in Plant Science, 2019
    Co-Authors: Sabrina Hupp, Katharina Bonfig, Thomas Roitsch, Maaria Rosenkranz, Chandana Pandey
    Abstract:

    Plant-Pathogen-Interactions have been widely studied, but mostly from the site of the Plant secondary defense. Less is known about the effects of Pathogen infection on Plant primary metabolism. The possibility to transform a fluorescing protein into prokaryotes is a promising phenotyping tool to follow a bacterial infection in Plants in a noninvasive manner. In the present study virulent and avirulent Pseudomonas syringae strains were transformed with Green Fluorescent Protein (GFP) to follow the spread of bacteria in vivo by imaging PAM fluorescence and conventional binocular microscopy. The combination of various wavelengths and filters allowed simultaneous detection of GFP transformed bacteria, PAM chlorophyll fluorescence and phenolic fluorescence from Pathogen infected Plant leaves. The results show that fluorescence imaging allows spatiotemporal monitoring of Pathogen spread as well as phenolic- and chlorophyll fluorescence in situ thus providing a novel means to study complex Plant-Pathogen Interactions and relate the responses of primary and secondary metabolism to Pathogen spread and multiplication. The study establishes a deeper understanding of imaging data and their implementation into disease screening.

  • Noninvasive Phenotyping of Plant-Pathogen Interaction: Consecutive In Situ Imaging of Fluorescing Pseudomonas syringae, Plant Phenolic Fluorescence, and Chlorophyll Fluorescence in Arabidopsis Leaves.
    Frontiers in plant science, 2019
    Co-Authors: Sabrina Hupp, Katharina Bonfig, Maaria Rosenkranz, Chandana Pandey, Thomas Roitsch
    Abstract:

    Plant-Pathogen Interactions have been widely studied, but mostly from the site of the Plant secondary defense. Less is known about the effects of Pathogen infection on Plant primary metabolism. The possibility to transform a fluorescing protein into prokaryotes is a promising phenotyping tool to follow a bacterial infection in Plants in a noninvasive manner. In the present study, virulent and avirulent Pseudomonas syringae strains were transformed with green fluorescent protein (GFP) to follow the spread of bacteria in vivo by imaging Pulse-Amplitude-Modulation (PAM) fluorescence and conventional binocular microscopy. The combination of various wavelengths and filters allowed simultaneous detection of GFP-transformed bacteria, PAM chlorophyll fluorescence, and phenolic fluorescence from Pathogen-infected Plant leaves. The results show that fluorescence imaging allows spatiotemporal monitoring of Pathogen spread as well as phenolic and chlorophyll fluorescence in situ, thus providing a novel means to study complex Plant-Pathogen Interactions and relate the responses of primary and secondary metabolism to Pathogen spread and multiplication. The study establishes a deeper understanding of imaging data and their implementation into disease screening.

  • visualization of dynamics of Plant Pathogen Interaction by novel combination of chlorophyll fluorescence imaging and statistical analysis differential effects of virulent and avirulent strains of p syringae and of oxylipins on a thaliana
    Journal of Experimental Botany, 2007
    Co-Authors: Susanne Berger, Zuzana Benediktyova, Katharina Bonfig, Martin J Mueller, Ladislav Nedbal, Karel Matous, Thomas Roitsch
    Abstract:

    Pathogen infection leads to defence induction as well as to changes in carbohydrate metabolism of Plants. Salicylic acid and oxylipins are involved in the induction of defence, but it is not known if these signalling molecules also mediate changes in carbohydrate metabolism. In this study, the effect of application of salicylic acid and the oxylipins 12-oxo-phytodienoic acid (OPDA) and jasmonic acid on photosynthesis was investigated by kinetic chlorophyll fluorescence imaging and compared with the effects of infection by virulent and avirulent strains of Pseudomonas syringae. Both Pathogen strains and OPDA caused a similar change in fluorescence parameters of leaves of Arabidopsis thaliana. The response to OPDA appeared faster compared with that to the Pathogens and persisted only for a short time. Infiltration with jasmonic acid or salicylic acid did not lead to a localized and distinct fluorescence response of the Plant. To capture the faint early symptoms of the Plant response, a novel algorithm was applied identifying the unique fluorescence signature—the set of images that, when combined, yield the highest contrast between control and infected leaf segments. Unlike conventional fluorescence parameters, this non-biased approach indeed detected the infection as early as 6 h after inoculation with bacteria. It was posssible to identify distinct fluorescence signatures characterizing the early and late phases of the infection. Fluorescence signatures of both infection phases were found in leaves infiltrated with OPDA.

Guoqiang Fan - One of the best experts on this subject based on the ideXlab platform.

  • Genome-wide analysis of three histone marks and gene expression in Paulownia fortunei with phytoplasma infection
    BMC Genomics, 2019
    Co-Authors: Lijun Yan, Guoqiang Fan, Xiaoyu Li
    Abstract:

    BackgroundPaulownia withes’-broom (PaWB) disease caused by phytoplasma is a serious infectious disease for Paulownia. However, the underlying molecular Pathogenesis is not fully understood. Recent studies have demonstrated that histone modifications could play a role in Plant defense responses to Pathogens. But there is still no available genome-wide histone modification data in non-model ligneous species infected with phytoplasma.ResultsHere, we provided the first genome-wide profiles of three histone marks (H3K4me3, H3K36me3 and H3K9ac) in Paulownia fortunei under phytoplasma stress by using chromatin immunoprecipitation sequencing (ChIP-Seq). We found that H3K4me3, H3K36me3 and H3K9ac were mainly enriched in the genic regions in P. fortunei with (PFI) and without (PF) phytoplasma infection. ChIP-Seq analysis revealed 1738, 986, and 2577 genes were differentially modified by H3K4me3, H3K36me3 and H3K9ac marks in PFI under phytoplasma infection, respectively. The functional analysis of these genes suggested that most of them were mainly involved in metabolic pathways, biosynthesis of secondary metabolites, phenylpropanoid biosynthesis, Plant-Pathogen Interaction and Plant hormone signal transduction. In addition, the combinational analysis of ChIP-Seq and RNA-Seq showed that differential histone methylation and acetylation only affected a small subset of phytoplasma-responsive genes.ConclusionsTaken together, this is the first report of integrated analysis of histone modifications and gene expression involved in Paulownia-phytoplasma Interaction. Our results will provide the valuable resources for the mechanism studies of gene regulation in non-model Plants upon Pathogens attack.

  • Plant-Pathogen Interaction-Related MicroRNAs and Their Targets Provide Indicators of Phytoplasma Infection in Paulownia tomentosa × Paulownia fortunei.
    PloS one, 2015
    Co-Authors: Guoqiang Fan, Suyan Niu, Minjie Deng, Zhenli Zhao, Yuanlong Wang, Lin Cao, Zhe Wang
    Abstract:

    Paulownia witches’ broom (PaWB) caused by a phytoplasma, has caused extensive losses in the yields of paulownia timber and resulted in significant economic losses. However, the molecular mechanisms in Paulownia that underlie the phytoplasma stress are poorly characterized. In this study, we use an Illumina platform to sequence four small RNA libraries and four degradome sequencing libraries derived from healthy, PaWB-infected, and PaWB-infected 15 mg·L−1 and 30 mg·L−1 methyl methane sulfonate (MMS)-treated Plants. In total, 125 conserved and 118 novel microRNAs (miRNAs) were identified and 33 miRNAs responsive to PaWB disease were discovered. Furthermore, 166 target genes for 18 PaWB disease-related miRNAs were obtained, and found to be involved in Plant-Pathogen Interaction and Plant hormone signal transduction metabolic pathways. Eleven miRNAs and target genes responsive to PaWB disease were examined by a quantitative real-time PCR approach. Our findings will contribute to studies on miRNAs and their targets in Paulownia, and provide new insights to further understand Plant-phytoplasma Interactions.

  • Plant Pathogen Interaction related micrornas and their targets provide indicators of phytoplasma infection in paulownia tomentosa paulownia fortunei
    PLOS ONE, 2015
    Co-Authors: Guoqiang Fan, Suyan Niu, Minjie Deng, Zhenli Zhao, Yuanlong Wang, Lin Cao, Zhe Wang
    Abstract:

    Paulownia witches’ broom (PaWB) caused by a phytoplasma, has caused extensive losses in the yields of paulownia timber and resulted in significant economic losses. However, the molecular mechanisms in Paulownia that underlie the phytoplasma stress are poorly characterized. In this study, we use an Illumina platform to sequence four small RNA libraries and four degradome sequencing libraries derived from healthy, PaWB-infected, and PaWB-infected 15 mg·L−1 and 30 mg·L−1 methyl methane sulfonate (MMS)-treated Plants. In total, 125 conserved and 118 novel microRNAs (miRNAs) were identified and 33 miRNAs responsive to PaWB disease were discovered. Furthermore, 166 target genes for 18 PaWB disease-related miRNAs were obtained, and found to be involved in Plant-Pathogen Interaction and Plant hormone signal transduction metabolic pathways. Eleven miRNAs and target genes responsive to PaWB disease were examined by a quantitative real-time PCR approach. Our findings will contribute to studies on miRNAs and their targets in Paulownia, and provide new insights to further understand Plant-phytoplasma Interactions.

  • Plant-Pathogen Interaction, circadian rhythm, and hormone-related gene expression provide indicators of phytoplasma infection in Paulownia fortunei.
    International journal of molecular sciences, 2014
    Co-Authors: Guoqiang Fan, Minjie Deng, Zhenli Zhao, Yanpeng Dong, Suyan Niu
    Abstract:

    Phytoplasmas are mycoplasma-like Pathogens of witches' broom disease, and are responsible for serious yield losses of Paulownia trees worldwide. The molecular mechanisms of disease development in Paulownia are of considerable interest, but still poorly understood. Here, we have applied transcriptome sequencing technology and a de novo assembly approach to analyze gene expression profiles in Paulownia fortunei infected by phytoplasmas. Our previous researches suggested that methyl methane sulfonated (MMS) could reverse the effects of the infection. In this study, leaf samples from healthy, infected, and both infected and methyl methane sulfonate treated Plants were analyzed. The results showed that the gene expression profile of P. fortunei underwent dramatic changes after Paulownia witches' broom (PaWB) phytoplasma infection. Genes that encoded key enzymes in Plant-Pathogen Interaction processes were significantly up-regulated in the PaWB-infected Paulownia. Genes involved in circadian rhythm and hormone-related genes were also altered in Paulownia after PaWB infection. However, after the PaWB-infected Plants were treated with MMS, the expression profiles of these genes returned to the levels in the healthy controls. The data will help identify potential PaWB disease-resistance genes that could be targeted to inhibit the growth and reproduction of the Pathogen and to increase Plant resistance.

Katharina Bonfig - One of the best experts on this subject based on the ideXlab platform.

  • noninvasive phenotyping of Plant Pathogen Interaction consecutive in situ imaging of fluorescing pseudomonas syringae Plant phenolic fluorescence and chlorophyll fluorescence in arabidopsis leaves
    Frontiers in Plant Science, 2019
    Co-Authors: Sabrina Hupp, Katharina Bonfig, Thomas Roitsch, Maaria Rosenkranz, Chandana Pandey
    Abstract:

    Plant-Pathogen-Interactions have been widely studied, but mostly from the site of the Plant secondary defense. Less is known about the effects of Pathogen infection on Plant primary metabolism. The possibility to transform a fluorescing protein into prokaryotes is a promising phenotyping tool to follow a bacterial infection in Plants in a noninvasive manner. In the present study virulent and avirulent Pseudomonas syringae strains were transformed with Green Fluorescent Protein (GFP) to follow the spread of bacteria in vivo by imaging PAM fluorescence and conventional binocular microscopy. The combination of various wavelengths and filters allowed simultaneous detection of GFP transformed bacteria, PAM chlorophyll fluorescence and phenolic fluorescence from Pathogen infected Plant leaves. The results show that fluorescence imaging allows spatiotemporal monitoring of Pathogen spread as well as phenolic- and chlorophyll fluorescence in situ thus providing a novel means to study complex Plant-Pathogen Interactions and relate the responses of primary and secondary metabolism to Pathogen spread and multiplication. The study establishes a deeper understanding of imaging data and their implementation into disease screening.

  • Noninvasive Phenotyping of Plant-Pathogen Interaction: Consecutive In Situ Imaging of Fluorescing Pseudomonas syringae, Plant Phenolic Fluorescence, and Chlorophyll Fluorescence in Arabidopsis Leaves.
    Frontiers in plant science, 2019
    Co-Authors: Sabrina Hupp, Katharina Bonfig, Maaria Rosenkranz, Chandana Pandey, Thomas Roitsch
    Abstract:

    Plant-Pathogen Interactions have been widely studied, but mostly from the site of the Plant secondary defense. Less is known about the effects of Pathogen infection on Plant primary metabolism. The possibility to transform a fluorescing protein into prokaryotes is a promising phenotyping tool to follow a bacterial infection in Plants in a noninvasive manner. In the present study, virulent and avirulent Pseudomonas syringae strains were transformed with green fluorescent protein (GFP) to follow the spread of bacteria in vivo by imaging Pulse-Amplitude-Modulation (PAM) fluorescence and conventional binocular microscopy. The combination of various wavelengths and filters allowed simultaneous detection of GFP-transformed bacteria, PAM chlorophyll fluorescence, and phenolic fluorescence from Pathogen-infected Plant leaves. The results show that fluorescence imaging allows spatiotemporal monitoring of Pathogen spread as well as phenolic and chlorophyll fluorescence in situ, thus providing a novel means to study complex Plant-Pathogen Interactions and relate the responses of primary and secondary metabolism to Pathogen spread and multiplication. The study establishes a deeper understanding of imaging data and their implementation into disease screening.

  • visualization of dynamics of Plant Pathogen Interaction by novel combination of chlorophyll fluorescence imaging and statistical analysis differential effects of virulent and avirulent strains of p syringae and of oxylipins on a thaliana
    Journal of Experimental Botany, 2007
    Co-Authors: Susanne Berger, Zuzana Benediktyova, Katharina Bonfig, Martin J Mueller, Ladislav Nedbal, Karel Matous, Thomas Roitsch
    Abstract:

    Pathogen infection leads to defence induction as well as to changes in carbohydrate metabolism of Plants. Salicylic acid and oxylipins are involved in the induction of defence, but it is not known if these signalling molecules also mediate changes in carbohydrate metabolism. In this study, the effect of application of salicylic acid and the oxylipins 12-oxo-phytodienoic acid (OPDA) and jasmonic acid on photosynthesis was investigated by kinetic chlorophyll fluorescence imaging and compared with the effects of infection by virulent and avirulent strains of Pseudomonas syringae. Both Pathogen strains and OPDA caused a similar change in fluorescence parameters of leaves of Arabidopsis thaliana. The response to OPDA appeared faster compared with that to the Pathogens and persisted only for a short time. Infiltration with jasmonic acid or salicylic acid did not lead to a localized and distinct fluorescence response of the Plant. To capture the faint early symptoms of the Plant response, a novel algorithm was applied identifying the unique fluorescence signature—the set of images that, when combined, yield the highest contrast between control and infected leaf segments. Unlike conventional fluorescence parameters, this non-biased approach indeed detected the infection as early as 6 h after inoculation with bacteria. It was posssible to identify distinct fluorescence signatures characterizing the early and late phases of the infection. Fluorescence signatures of both infection phases were found in leaves infiltrated with OPDA.

Ling Yin - One of the best experts on this subject based on the ideXlab platform.

  • Linkage of cold acclimation and disease resistance through Plant–Pathogen Interaction pathway in Vitis amurensis grapevine
    Functional & integrative genomics, 2014
    Co-Authors: Yali Zhang, Ling Yin
    Abstract:

    Low temperatures cause severe damage to none cold hardy grapevines. A preliminary survey with Solexa sequencing technology was used to analyze gene expression profiles of cold hardy Vitis amurensis ‘Zuoshan-1’ after cold acclimation at 4 °C for 48 h. A total of 16,750 and 18,068 putative genes were annotated for 4 °C-treated and control library, respectively. Among them, 393 genes were upregulated for at least 20-fold, while 69 genes were downregulated for at least 20-fold under the 4 °C treatment for 48 h. A subset of 101 genes from this survey was investigated further using reverse transcription polymerase chain reaction (RT-PCR). Genes associated with signaling events in Pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI), including generation of calcium signals (CNGC, CMLs), jasmonic acid signal (JAZ1), oxidative burst (Rboh), and phosphorylation (FLS2, BAK, MEKK1, MKKs) cascades, were upregulated after cold acclimation. Disease resistance genes (RPM1, RPS5, RIN4, PBS1) in the process of effector-triggered immunity (ETI) were also upregulated in the current condition. Defense-related genes (WRKYs, PR1, MIN7) involved in both PTI and ETI processes were abundantly expressed after cold acclimation. Our results indicated that Plant–Pathogen Interaction pathways were linked to the cold acclimation in V. amurensis grapevine. Other biotic- and abiotic-related genes, such as defense (protein phosphatase 2C, U-box domain proteins, NCED1, stilbene synthase), transcription (DREBs, MYBs, ERFs, ZFPs), signal transduction (kinase, calcium, and auxin signaling), transport (ATP-binding cassette (ABC) transporters, auxin:hydrogen symporter), and various metabolism, were also abundantly expressed in the cold acclimation of V. Amurensis ‘Zuoshan-1’ grapevine. This study revealed a series of critical genes and pathways to delineate important biological processes affected by low temperature in ‘Zuoshan-1’.

  • linkage of cold acclimation and disease resistance through Plant Pathogen Interaction pathway in vitis amurensis grapevine
    Functional & Integrative Genomics, 2014
    Co-Authors: Yali Zhang, Ling Yin
    Abstract:

    Low temperatures cause severe damage to none cold hardy grapevines. A preliminary survey with Solexa sequencing technology was used to analyze gene expression profiles of cold hardy Vitis amurensis ‘Zuoshan-1’ after cold acclimation at 4 °C for 48 h. A total of 16,750 and 18,068 putative genes were annotated for 4 °C-treated and control library, respectively. Among them, 393 genes were upregulated for at least 20-fold, while 69 genes were downregulated for at least 20-fold under the 4 °C treatment for 48 h. A subset of 101 genes from this survey was investigated further using reverse transcription polymerase chain reaction (RT-PCR). Genes associated with signaling events in Pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI), including generation of calcium signals (CNGC, CMLs), jasmonic acid signal (JAZ1), oxidative burst (Rboh), and phosphorylation (FLS2, BAK, MEKK1, MKKs) cascades, were upregulated after cold acclimation. Disease resistance genes (RPM1, RPS5, RIN4, PBS1) in the process of effector-triggered immunity (ETI) were also upregulated in the current condition. Defense-related genes (WRKYs, PR1, MIN7) involved in both PTI and ETI processes were abundantly expressed after cold acclimation. Our results indicated that Plant–Pathogen Interaction pathways were linked to the cold acclimation in V. amurensis grapevine. Other biotic- and abiotic-related genes, such as defense (protein phosphatase 2C, U-box domain proteins, NCED1, stilbene synthase), transcription (DREBs, MYBs, ERFs, ZFPs), signal transduction (kinase, calcium, and auxin signaling), transport (ATP-binding cassette (ABC) transporters, auxin:hydrogen symporter), and various metabolism, were also abundantly expressed in the cold acclimation of V. Amurensis ‘Zuoshan-1’ grapevine. This study revealed a series of critical genes and pathways to delineate important biological processes affected by low temperature in ‘Zuoshan-1’.

Sabrina Hupp - One of the best experts on this subject based on the ideXlab platform.

  • Noninvasive Phenotyping of Plant-Pathogen Interaction: Consecutive In Situ Imaging of Fluorescing Pseudomonas syringae, Plant Phenolic Fluorescence, and Chlorophyll Fluorescence in Arabidopsis Leaves.
    Frontiers in plant science, 2019
    Co-Authors: Sabrina Hupp, Katharina Bonfig, Maaria Rosenkranz, Chandana Pandey, Thomas Roitsch
    Abstract:

    Plant-Pathogen Interactions have been widely studied, but mostly from the site of the Plant secondary defense. Less is known about the effects of Pathogen infection on Plant primary metabolism. The possibility to transform a fluorescing protein into prokaryotes is a promising phenotyping tool to follow a bacterial infection in Plants in a noninvasive manner. In the present study, virulent and avirulent Pseudomonas syringae strains were transformed with green fluorescent protein (GFP) to follow the spread of bacteria in vivo by imaging Pulse-Amplitude-Modulation (PAM) fluorescence and conventional binocular microscopy. The combination of various wavelengths and filters allowed simultaneous detection of GFP-transformed bacteria, PAM chlorophyll fluorescence, and phenolic fluorescence from Pathogen-infected Plant leaves. The results show that fluorescence imaging allows spatiotemporal monitoring of Pathogen spread as well as phenolic and chlorophyll fluorescence in situ, thus providing a novel means to study complex Plant-Pathogen Interactions and relate the responses of primary and secondary metabolism to Pathogen spread and multiplication. The study establishes a deeper understanding of imaging data and their implementation into disease screening.

  • noninvasive phenotyping of Plant Pathogen Interaction consecutive in situ imaging of fluorescing pseudomonas syringae Plant phenolic fluorescence and chlorophyll fluorescence in arabidopsis leaves
    Frontiers in Plant Science, 2019
    Co-Authors: Sabrina Hupp, Katharina Bonfig, Thomas Roitsch, Maaria Rosenkranz, Chandana Pandey
    Abstract:

    Plant-Pathogen-Interactions have been widely studied, but mostly from the site of the Plant secondary defense. Less is known about the effects of Pathogen infection on Plant primary metabolism. The possibility to transform a fluorescing protein into prokaryotes is a promising phenotyping tool to follow a bacterial infection in Plants in a noninvasive manner. In the present study virulent and avirulent Pseudomonas syringae strains were transformed with Green Fluorescent Protein (GFP) to follow the spread of bacteria in vivo by imaging PAM fluorescence and conventional binocular microscopy. The combination of various wavelengths and filters allowed simultaneous detection of GFP transformed bacteria, PAM chlorophyll fluorescence and phenolic fluorescence from Pathogen infected Plant leaves. The results show that fluorescence imaging allows spatiotemporal monitoring of Pathogen spread as well as phenolic- and chlorophyll fluorescence in situ thus providing a novel means to study complex Plant-Pathogen Interactions and relate the responses of primary and secondary metabolism to Pathogen spread and multiplication. The study establishes a deeper understanding of imaging data and their implementation into disease screening.