The Experts below are selected from a list of 44616 Experts worldwide ranked by ideXlab platform

Tetsuya Higashiyama - One of the best experts on this subject based on the ideXlab platform.

  • Arabinogalactan proteins and their sugar chains: functions in Plant Reproduction, research methods, and biosynthesis.
    Plant reproduction, 2018
    Co-Authors: Tetsuya Higashiyama
    Abstract:

    The arabinogalactan protein (AGP) family is one of the most complex protein families and is ubiquitous in the Plant kingdom. Moreover, it has been demonstrated to play various roles during Plant Reproduction. A typical AGP contains a hydroxyproline-rich core protein with high heterogeneity and varying numbers of polysaccharide side chains. However, the functions of the polysaccharide components (i.e. AG sugar chains) remain largely unknown due to the general difficulties associated with studying sugar chains in glycobiology. In recent years, methodological breakthroughs have resulted in substantial progress in AGP research. Here, we summarise the multiple roles of AGPs during Plant gametophyte development and male-female communication, with a focus on recent advances. In addition, we discuss the analytical tools used in AGP research, and the biosynthesis and function of AG sugar chains. A comprehensive understanding of the AGP family will help clarify the mechanisms precisely controlling reproductive processes.

  • Poly(dimethylsiloxane)-based microdevices for studying Plant Reproduction.
    Biochemical Society Transactions, 2014
    Co-Authors: Hideyuki Arata, Tetsuya Higashiyama
    Abstract:

    Long-term holding and precise handling of growing Plant tissues during in vitro cultivation has been a major hurdle for experimental studies related to Plant development and Reproduction. In the present review, we introduce two of our newly developed poly(dimethylsiloxane)-based microdevices: a T-shaped microchannel device for pollen tube chemoattraction and a microcage array for long-term live imaging of ovules. Their design, usage and advantages are described, and future prospects of experimental approaches to Plant Reproduction using such microdevices are discussed.

  • live cell analysis of Plant Reproduction live cell imaging optical manipulation and advanced microscopy technologies
    Development Growth & Differentiation, 2013
    Co-Authors: Daisuke Kurihara, Yuki Hamamura, Tetsuya Higashiyama
    Abstract:

    Sexual Reproduction ensures propagation of species and enhances genetic diversity within populations. In flowering Plants, sexual Reproduction requires complicated and multi-step cell-to-cell communications among male and female cells. However, the confined nature of Plant Reproduction processes, which occur in the female reproductive organs and several cell layers of the pistil, limits our ability to observe these events in vivo. In this review, we discuss recent live-cell imaging in in vitro systems and the optical manipulation techniques that are used to capture the dynamic mechanisms representing molecular and cellular communications in sexual Plant Reproduction.

  • Live‐cell analysis of Plant Reproduction: Live‐cell imaging, optical manipulation, and advanced microscopy technologies
    Development growth & differentiation, 2013
    Co-Authors: Daisuke Kurihara, Yuki Hamamura, Tetsuya Higashiyama
    Abstract:

    Sexual Reproduction ensures propagation of species and enhances genetic diversity within populations. In flowering Plants, sexual Reproduction requires complicated and multi-step cell-to-cell communications among male and female cells. However, the confined nature of Plant Reproduction processes, which occur in the female reproductive organs and several cell layers of the pistil, limits our ability to observe these events in vivo. In this review, we discuss recent live-cell imaging in in vitro systems and the optical manipulation techniques that are used to capture the dynamic mechanisms representing molecular and cellular communications in sexual Plant Reproduction.

Martin Bayer - One of the best experts on this subject based on the ideXlab platform.

  • A simple and versatile cell wall staining protocol to study Plant Reproduction
    Plant Reproduction, 2015
    Co-Authors: Thomas J Musielak, Agnes Henschen, Laura Schenkel, Martina Kolb, Martin Bayer
    Abstract:

    Key message The optical brightener SCRI Renaissance 2200 can be used as versatile dye to study various aspects of Plant Reproduction by confocal laser scanning microscopy. Abstract The study of sexual Reproduction of Plants has traditionally relied on light microscopy in combination with a variety of staining methods. Transgenic lines that label specific cell or tissue types with fluorescent proteins in combination with confocal laser scanning microscopy were an important development to visualize gametophyte development, the fertilization process, and to follow cell differentiation in the early embryo. Staining the cell perimeter to identify surrounding tissue is often a necessary prerequisite to put the fluorescent signal in the right context. Here, we present SCRI Renaissance 2200 (SR2200) as a versatile dye to study various aspects of Plant Reproduction ranging from pollen tube growth, guidance and reception to the early patterning process in the developing embryo of Arabidopsis thaliana . Furthermore, we demonstrate that SR2200 can be combined with a wide variety of fluorescent proteins. If spectral information can be recorded, even double labeling with dyes that have very similar emission spectra such as 4′,6-diamidin-2-phenylindol (DAPI) is possible. The presented staining method can be a single, easy-to-use alternative for a range of other staining protocols commonly used for microscopic analyses in Plant reproductive biology.

  • A simple and versatile cell wall staining protocol to study Plant Reproduction.
    Plant reproduction, 2015
    Co-Authors: Thomas J Musielak, Agnes Henschen, Laura Schenkel, Martina Kolb, Martin Bayer
    Abstract:

    The optical brightener SCRI Renaissance 2200 can be used as versatile dye to study various aspects of Plant Reproduction by confocal laser scanning microscopy. The study of sexual Reproduction of Plants has traditionally relied on light microscopy in combination with a variety of staining methods. Transgenic lines that label specific cell or tissue types with fluorescent proteins in combination with confocal laser scanning microscopy were an important development to visualize gametophyte development, the fertilization process, and to follow cell differentiation in the early embryo. Staining the cell perimeter to identify surrounding tissue is often a necessary prerequisite to put the fluorescent signal in the right context. Here, we present SCRI Renaissance 2200 (SR2200) as a versatile dye to study various aspects of Plant Reproduction ranging from pollen tube growth, guidance and reception to the early patterning process in the developing embryo of Arabidopsis thaliana. Furthermore, we demonstrate that SR2200 can be combined with a wide variety of fluorescent proteins. If spectral information can be recorded, even double labeling with dyes that have very similar emission spectra such as 4',6-diamidin-2-phenylindol (DAPI) is possible. The presented staining method can be a single, easy-to-use alternative for a range of other staining protocols commonly used for microscopic analyses in Plant reproductive biology.

Thomas J Musielak - One of the best experts on this subject based on the ideXlab platform.

  • A simple and versatile cell wall staining protocol to study Plant Reproduction
    Plant Reproduction, 2015
    Co-Authors: Thomas J Musielak, Agnes Henschen, Laura Schenkel, Martina Kolb, Martin Bayer
    Abstract:

    Key message The optical brightener SCRI Renaissance 2200 can be used as versatile dye to study various aspects of Plant Reproduction by confocal laser scanning microscopy. Abstract The study of sexual Reproduction of Plants has traditionally relied on light microscopy in combination with a variety of staining methods. Transgenic lines that label specific cell or tissue types with fluorescent proteins in combination with confocal laser scanning microscopy were an important development to visualize gametophyte development, the fertilization process, and to follow cell differentiation in the early embryo. Staining the cell perimeter to identify surrounding tissue is often a necessary prerequisite to put the fluorescent signal in the right context. Here, we present SCRI Renaissance 2200 (SR2200) as a versatile dye to study various aspects of Plant Reproduction ranging from pollen tube growth, guidance and reception to the early patterning process in the developing embryo of Arabidopsis thaliana . Furthermore, we demonstrate that SR2200 can be combined with a wide variety of fluorescent proteins. If spectral information can be recorded, even double labeling with dyes that have very similar emission spectra such as 4′,6-diamidin-2-phenylindol (DAPI) is possible. The presented staining method can be a single, easy-to-use alternative for a range of other staining protocols commonly used for microscopic analyses in Plant reproductive biology.

  • a simple and versatile cell wall staining protocol to study Plant Reproduction
    Sexual Plant Reproduction, 2015
    Co-Authors: Thomas J Musielak, Laura Schenkel, Martina Kolb, Agnes Hensche, Marti Aye
    Abstract:

    Key message The optical brightener SCRI Renaissance 2200 can be used as versatile dye to study various aspects of Plant Reproduction by confocal laser scanning microscopy.

  • A simple and versatile cell wall staining protocol to study Plant Reproduction.
    Plant reproduction, 2015
    Co-Authors: Thomas J Musielak, Agnes Henschen, Laura Schenkel, Martina Kolb, Martin Bayer
    Abstract:

    The optical brightener SCRI Renaissance 2200 can be used as versatile dye to study various aspects of Plant Reproduction by confocal laser scanning microscopy. The study of sexual Reproduction of Plants has traditionally relied on light microscopy in combination with a variety of staining methods. Transgenic lines that label specific cell or tissue types with fluorescent proteins in combination with confocal laser scanning microscopy were an important development to visualize gametophyte development, the fertilization process, and to follow cell differentiation in the early embryo. Staining the cell perimeter to identify surrounding tissue is often a necessary prerequisite to put the fluorescent signal in the right context. Here, we present SCRI Renaissance 2200 (SR2200) as a versatile dye to study various aspects of Plant Reproduction ranging from pollen tube growth, guidance and reception to the early patterning process in the developing embryo of Arabidopsis thaliana. Furthermore, we demonstrate that SR2200 can be combined with a wide variety of fluorescent proteins. If spectral information can be recorded, even double labeling with dyes that have very similar emission spectra such as 4',6-diamidin-2-phenylindol (DAPI) is possible. The presented staining method can be a single, easy-to-use alternative for a range of other staining protocols commonly used for microscopic analyses in Plant reproductive biology.

Martina Kolb - One of the best experts on this subject based on the ideXlab platform.

  • A simple and versatile cell wall staining protocol to study Plant Reproduction
    Plant Reproduction, 2015
    Co-Authors: Thomas J Musielak, Agnes Henschen, Laura Schenkel, Martina Kolb, Martin Bayer
    Abstract:

    Key message The optical brightener SCRI Renaissance 2200 can be used as versatile dye to study various aspects of Plant Reproduction by confocal laser scanning microscopy. Abstract The study of sexual Reproduction of Plants has traditionally relied on light microscopy in combination with a variety of staining methods. Transgenic lines that label specific cell or tissue types with fluorescent proteins in combination with confocal laser scanning microscopy were an important development to visualize gametophyte development, the fertilization process, and to follow cell differentiation in the early embryo. Staining the cell perimeter to identify surrounding tissue is often a necessary prerequisite to put the fluorescent signal in the right context. Here, we present SCRI Renaissance 2200 (SR2200) as a versatile dye to study various aspects of Plant Reproduction ranging from pollen tube growth, guidance and reception to the early patterning process in the developing embryo of Arabidopsis thaliana . Furthermore, we demonstrate that SR2200 can be combined with a wide variety of fluorescent proteins. If spectral information can be recorded, even double labeling with dyes that have very similar emission spectra such as 4′,6-diamidin-2-phenylindol (DAPI) is possible. The presented staining method can be a single, easy-to-use alternative for a range of other staining protocols commonly used for microscopic analyses in Plant reproductive biology.

  • a simple and versatile cell wall staining protocol to study Plant Reproduction
    Sexual Plant Reproduction, 2015
    Co-Authors: Thomas J Musielak, Laura Schenkel, Martina Kolb, Agnes Hensche, Marti Aye
    Abstract:

    Key message The optical brightener SCRI Renaissance 2200 can be used as versatile dye to study various aspects of Plant Reproduction by confocal laser scanning microscopy.

  • A simple and versatile cell wall staining protocol to study Plant Reproduction.
    Plant reproduction, 2015
    Co-Authors: Thomas J Musielak, Agnes Henschen, Laura Schenkel, Martina Kolb, Martin Bayer
    Abstract:

    The optical brightener SCRI Renaissance 2200 can be used as versatile dye to study various aspects of Plant Reproduction by confocal laser scanning microscopy. The study of sexual Reproduction of Plants has traditionally relied on light microscopy in combination with a variety of staining methods. Transgenic lines that label specific cell or tissue types with fluorescent proteins in combination with confocal laser scanning microscopy were an important development to visualize gametophyte development, the fertilization process, and to follow cell differentiation in the early embryo. Staining the cell perimeter to identify surrounding tissue is often a necessary prerequisite to put the fluorescent signal in the right context. Here, we present SCRI Renaissance 2200 (SR2200) as a versatile dye to study various aspects of Plant Reproduction ranging from pollen tube growth, guidance and reception to the early patterning process in the developing embryo of Arabidopsis thaliana. Furthermore, we demonstrate that SR2200 can be combined with a wide variety of fluorescent proteins. If spectral information can be recorded, even double labeling with dyes that have very similar emission spectra such as 4',6-diamidin-2-phenylindol (DAPI) is possible. The presented staining method can be a single, easy-to-use alternative for a range of other staining protocols commonly used for microscopic analyses in Plant reproductive biology.

Laura Schenkel - One of the best experts on this subject based on the ideXlab platform.

  • A simple and versatile cell wall staining protocol to study Plant Reproduction
    Plant Reproduction, 2015
    Co-Authors: Thomas J Musielak, Agnes Henschen, Laura Schenkel, Martina Kolb, Martin Bayer
    Abstract:

    Key message The optical brightener SCRI Renaissance 2200 can be used as versatile dye to study various aspects of Plant Reproduction by confocal laser scanning microscopy. Abstract The study of sexual Reproduction of Plants has traditionally relied on light microscopy in combination with a variety of staining methods. Transgenic lines that label specific cell or tissue types with fluorescent proteins in combination with confocal laser scanning microscopy were an important development to visualize gametophyte development, the fertilization process, and to follow cell differentiation in the early embryo. Staining the cell perimeter to identify surrounding tissue is often a necessary prerequisite to put the fluorescent signal in the right context. Here, we present SCRI Renaissance 2200 (SR2200) as a versatile dye to study various aspects of Plant Reproduction ranging from pollen tube growth, guidance and reception to the early patterning process in the developing embryo of Arabidopsis thaliana . Furthermore, we demonstrate that SR2200 can be combined with a wide variety of fluorescent proteins. If spectral information can be recorded, even double labeling with dyes that have very similar emission spectra such as 4′,6-diamidin-2-phenylindol (DAPI) is possible. The presented staining method can be a single, easy-to-use alternative for a range of other staining protocols commonly used for microscopic analyses in Plant reproductive biology.

  • a simple and versatile cell wall staining protocol to study Plant Reproduction
    Sexual Plant Reproduction, 2015
    Co-Authors: Thomas J Musielak, Laura Schenkel, Martina Kolb, Agnes Hensche, Marti Aye
    Abstract:

    Key message The optical brightener SCRI Renaissance 2200 can be used as versatile dye to study various aspects of Plant Reproduction by confocal laser scanning microscopy.

  • A simple and versatile cell wall staining protocol to study Plant Reproduction.
    Plant reproduction, 2015
    Co-Authors: Thomas J Musielak, Agnes Henschen, Laura Schenkel, Martina Kolb, Martin Bayer
    Abstract:

    The optical brightener SCRI Renaissance 2200 can be used as versatile dye to study various aspects of Plant Reproduction by confocal laser scanning microscopy. The study of sexual Reproduction of Plants has traditionally relied on light microscopy in combination with a variety of staining methods. Transgenic lines that label specific cell or tissue types with fluorescent proteins in combination with confocal laser scanning microscopy were an important development to visualize gametophyte development, the fertilization process, and to follow cell differentiation in the early embryo. Staining the cell perimeter to identify surrounding tissue is often a necessary prerequisite to put the fluorescent signal in the right context. Here, we present SCRI Renaissance 2200 (SR2200) as a versatile dye to study various aspects of Plant Reproduction ranging from pollen tube growth, guidance and reception to the early patterning process in the developing embryo of Arabidopsis thaliana. Furthermore, we demonstrate that SR2200 can be combined with a wide variety of fluorescent proteins. If spectral information can be recorded, even double labeling with dyes that have very similar emission spectra such as 4',6-diamidin-2-phenylindol (DAPI) is possible. The presented staining method can be a single, easy-to-use alternative for a range of other staining protocols commonly used for microscopic analyses in Plant reproductive biology.