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García García, Pedro Daniel - One of the best experts on this subject based on the ideXlab platform.

  • Efecto Antibacteriano in Vitro del Aceite Esencial de Plantago Major “Llantén” sobre Staphylococcus Aureus ATCC 29213 comparado con Ciprofloxacino
    'Universidad Cesar Vallejo', 2019
    Co-Authors: García García, Pedro Daniel
    Abstract:

    TesisTrujilloEscuela Académico Profesional de MedicinaEnfermedades Infecciosas y TransmisiblesSe realizó un estudio experimental in vitro con el objetivo de evaluar el efecto antibacteriano del aceite esencial de la hoja de Plantago Major (Llantén) comparado con ciprofloxacino a la concentración de 5 µg, sobre cepas de Staphylococcus aureus ATCC 29213. Se realizaron cuatro diluciones del aceite esencial (100%, 75%, 50% y 25%) y un control neutro con DMSO; se realizaron 13 repeticiones por cada grupo de estudio. Se obtuvo que el aceite esencial de la hoja de Plantago Major muestra halos de inhibición a partir de la dilución al 75 % (16.4 mm DS ± 0.9 IC 95% 15-18) al 100% el halo de inhibición fue de 18.4mmm DS ± 1.0 IC 95% 17 - 20, valores no considerados como eficaces en relación al patrón del CLSI (>21mm), y no superan el halo de inhibición del ciprofloxacino (34.6 mm DS: ± 0.9 IC 95% 33 – 36). El análisis estadístico ANOVA indicó que los resultados del estudio fueron altamente significativos (p = 0.000), al igual que la prueba de Tukey demostró que los grupos evaluados eran homogéneos y el grupo de ciprofloxacino tenía mayor efecto antibacteriano. Se observa que a mayor concentración del aceite esencial de Plantago Major, el halo de inhibición aumenta. Se concluye que el aceite esencial de la hoja de Plantago Major tiene efecto antibacteriano sobre Staphylococcus aureus ATCC 29213, pero menor que ciprofloxacino, pero se podrá utilizar como un medicamento coadyuvante en el tratamiento de Staphylococcus aureus

  • Efecto Antibacteriano in Vitro del Aceite Esencial de Plantago Major “Llantén” sobre Staphylococcus Aureus ATCC 29213 comparado con Ciprofloxacino
    'Universidad Cesar Vallejo', 2019
    Co-Authors: García García, Pedro Daniel
    Abstract:

    Se realizó un estudio experimental in vitro con el objetivo de evaluar el efecto antibacteriano del aceite esencial de la hoja de Plantago Major (Llantén) comparado con ciprofloxacino a la concentración de 5 µg, sobre cepas de Staphylococcus aureus ATCC 29213. Se realizaron cuatro diluciones del aceite esencial (100%, 75%, 50% y 25%) y un control neutro con DMSO; se realizaron 13 repeticiones por cada grupo de estudio. Se obtuvo que el aceite esencial de la hoja de Plantago Major muestra halos de inhibición a partir de la dilución al 75 % (16.4 mm DS ± 0.9 IC 95% 15-18) al 100% el halo de inhibición fue de 18.4mmm DS ± 1.0 IC 95% 17 - 20, valores no considerados como eficaces en relación al patrón del CLSI (>21mm), y no superan el halo de inhibición del ciprofloxacino (34.6 mm DS: ± 0.9 IC 95% 33 – 36). El análisis estadístico ANOVA indicó que los resultados del estudio fueron altamente significativos (p = 0.000), al igual que la prueba de Tukey demostró que los grupos evaluados eran homogéneos y el grupo de ciprofloxacino tenía mayor efecto antibacteriano. Se observa que a mayor concentración del aceite esencial de Plantago Major, el halo de inhibición aumenta. Se concluye que el aceite esencial de la hoja de Plantago Major tiene efecto antibacteriano sobre Staphylococcus aureus ATCC 29213, pero menor que ciprofloxacino, pero se podrá utilizar como un medicamento coadyuvante en el tratamiento de Staphylococcus aureus

Kirsten Wolff - One of the best experts on this subject based on the ideXlab platform.

  • Isolation of polymorphic microsatellite loci in Plantago Major and P. intermedia
    Molecular Ecology Notes, 2001
    Co-Authors: J. Squirrell, Kirsten Wolff
    Abstract:

    Plantago Major and P. intermedia are two closely related inbreeding species. The isolation of polymorphic codominant microsatellite markers will provide valuable tools to investigate the reproductive isolation and the evolution of the two species. The isolation of microsatellite loci was achieved using a membrane enrichment method. Primers were designed to microsatellite flanking sequences and were analysed using fluorescent labels. Results indicated that nine out of the 10 loci amplified in both species, and that all the loci were polymorphic. The amplification of the loci was tested in a variety of Plantago species and was shown to be limited.

  • Genetic structure and differentiation of Plantago Major reveals a pair of sympatric sister species
    Molecular Ecology, 1999
    Co-Authors: Mary Morgan-richards, Kirsten Wolff
    Abstract:

    Seeds of the widespread weed Plantago Major were collected from 10 European countries, as well as Trinidad and North America. The seed collections were from populations of two taxa which are ecologically rather than geographically separated and formally recognized as the subspecies Plantago Major ssp. Major and P.m. ssp. intermedia (also called P.m. ssp. pleiosperma). Eight polymorphic allozyme loci and 73 random-primed DNA fragments were scored, as well as 11 morphological characters. Complete concordance between morphological traits and genetic data provides evidence that these two taxa, although very similar, are distinct species. They are both widespread, they are broadly sympatric and capable of interbreeding. However, slight morphological and ecological differences coincide with genetic clustering of populations from widely separated locations. In addition, P. Major and P. intermedia differ in their population structure: P. intermedia has greater genetic diversity among populations and less genetic variance within populations than P. Major. We suggest that differences between the two species in their levels of selfing may explain the distinctive genetic structure of each species. We hypothesize a link between selfing rate and lifespan of the two taxa. P. Major is characterized by lower genetic variation among populations, a higher rate of outcrossing, longer lifespan and production of fewer seeds per seed capsule. P. intermedia is more highly structured with much differentiation among populations, a higher rate of inbreeding and it often grows as an annual.

  • PCR markers distinguish Plantago Major subspecies
    Theoretical and Applied Genetics, 1998
    Co-Authors: Kirsten Wolff, Mary Morgan-richards
    Abstract:

    Plantago Major plants from several Scottish and Dutch locations were surveyed for their genetic variation using PCR markers, namely RAPD analysis, anchored inter-SSR PCR, and chloroplast PCR followed by RFLP analysis. The RAPD and inter-SSR markers showed a differentiation between the two subspecies of P. Major. These results are discussed in relation to earlier results using allozyme electrophoresis, DNA fingerprinting, and chloroplast RFLP analysis.

Muhammad Zubair - One of the best experts on this subject based on the ideXlab platform.

  • Water and ethanol extracts of Plantago Major leaves show anti-inflammatory activity on oral epithelial cells
    Journal of traditional and complementary medicine, 2018
    Co-Authors: Muhammad Zubair, Stefan Renvert, Cecilia Widén, Kimmo Rumpunen
    Abstract:

    Abstract Context The leaves of Plantago Major have been used for the treatment of wounds and inflammation in folk medicine from prehistoric times. However there is no report on the use of P. Major to treat inflammation in oral epithelial cell lines. Objective The present study was undertaken to reveal possible anti-inflammatory effects of Plantago Major leaf extracts on oral epithelial cells in-vitro. Materials and methods Water- and ethanol-based extracts of P. Major leaves were prepared from freeze-dried plant material, and tested in-vitro using the oral epithelial cell line H400. The anti-inflammatory activity of P. Major was tested against E. coli lipopolysaccharide (LPS) using the nuclear factor kappa beta (NF-kB) assay. Results Both the water- and the ethanol-based extracts, as well as a combination of the two extracts, showed anti-inflammatory activity. A concentration of 0.1 mg/mL (on dry weight basis) yielded the best results for all extracts. Discussion and conclusion The results show that synergistic effects of both polyphenols and water-soluble compounds (possibly polysaccharides) are responsible for anti-inflammatory activities of P. Major.

  • Effects of Plantago Major L. leaf extracts on oral epithelial cells in a scratch assay.
    Journal of ethnopharmacology, 2012
    Co-Authors: Muhammad Zubair, Anders Ekholm, Hilde Nybom, Stefan Renvert, Cecilia Widén, Kimmo Rumpunen
    Abstract:

    Aim of study The present study was undertaken to evaluate the effects from different leaf extracts of the traditional medicinal herb Plantago Major L. (plantain) on cell proliferation and migration ...

  • Genetic and environmental effects on polyphenols in Plantago Major
    2010
    Co-Authors: Muhammad Zubair
    Abstract:

    Leaves and seeds of Plantago Major (common plantain or greater plantain) have been used for centuries to treat diseases relating to skin, digestive organs and blood circulation like wounds, inflammation and hypertension. Either whole or crushed leaves have been used to treat for example burns and all kinds of wounds to enhance the healing process, and to stop bleeding. To treat superficial wounds it is sufficient to apply the juice from the leaves. Both polysaccharides and polyphenols may have a synergistic effect on wound healing and other biological activities. Polyphenols extracted from leaves and seeds of P. Major have been reported to have bioactive effects especially on wound healing, and to have antiulcerogenic, anti-inflammatory, antioxidant, anticarcinogenic and antiviral activity. Three subspecies have been described of P. Major, two of which have been subjected to genetic and phytochemical analysis. Plantago Major subsp. Major is naturalized almost throughout the world and is mainly found as an agronomic weed. There has been little work emphasizing the utilization of the bioactive compounds from P. Major in modern medicine. Similarly, the effects of genetic and environmental factors on the occurrence of these bioactive compounds have not been reported. The main emphasis of the introductor y paper is to highlight some factors that may be important for the utilization of Plantago Major as a medicinal herb, providing the scope for the Ph.D. study. This paper also describes the taxonomy including morphological differences between the two subspecies, distribution, biology, genetics and DNA markers used in P. Major.

  • GENETIC AND PHYTOCHEMICAL VARIATION IN Plantago Major
    African Journal of Traditional Complementary and Alternative Medicines, 2009
    Co-Authors: Muhammad Zubair
    Abstract:

    Leaves of the common weed Plantago Major (common plantain) have been used as a wound healing remedy in almost all parts of the world, and in the treatment of a number of other diseases in folk medicine. The biological activities of P. Major leaves are wound healing, anti-inflammatory, analgesic, antioxidant, weakly antibiotic, immuno-modulating and anti-ulcerogenic. The aim of study is to develop: 1. Plantago Major, well-known from folk medicine, into a modern medicinal herb, 2. Optimize organic cultivation procedures to produce leaf extracts for use in a superior, biologically active wound dressing. DNA analyses (RAPD) will be conducted for determining genetic variation among populations and for checking homogeneity in offspring from selected mother plants. Chemical analyses (HPLC and mass chromatography) will be performed in order to study different polyphenolic compounds (flavonoids, phenylpropanoid glycosides and iridoids) that are likely to be involvd in the wound-healing ability of P. Major. Greenhouse cultvation experiments will be undertaken to check the effects of different levels of light supplementation, heating and fertilization.

Kimmo Rumpunen - One of the best experts on this subject based on the ideXlab platform.

  • Water and ethanol extracts of Plantago Major leaves show anti-inflammatory activity on oral epithelial cells
    Journal of traditional and complementary medicine, 2018
    Co-Authors: Muhammad Zubair, Stefan Renvert, Cecilia Widén, Kimmo Rumpunen
    Abstract:

    Abstract Context The leaves of Plantago Major have been used for the treatment of wounds and inflammation in folk medicine from prehistoric times. However there is no report on the use of P. Major to treat inflammation in oral epithelial cell lines. Objective The present study was undertaken to reveal possible anti-inflammatory effects of Plantago Major leaf extracts on oral epithelial cells in-vitro. Materials and methods Water- and ethanol-based extracts of P. Major leaves were prepared from freeze-dried plant material, and tested in-vitro using the oral epithelial cell line H400. The anti-inflammatory activity of P. Major was tested against E. coli lipopolysaccharide (LPS) using the nuclear factor kappa beta (NF-kB) assay. Results Both the water- and the ethanol-based extracts, as well as a combination of the two extracts, showed anti-inflammatory activity. A concentration of 0.1 mg/mL (on dry weight basis) yielded the best results for all extracts. Discussion and conclusion The results show that synergistic effects of both polyphenols and water-soluble compounds (possibly polysaccharides) are responsible for anti-inflammatory activities of P. Major.

  • Effects of Plantago Major L. leaf extracts on oral epithelial cells in a scratch assay.
    Journal of ethnopharmacology, 2012
    Co-Authors: Muhammad Zubair, Anders Ekholm, Hilde Nybom, Stefan Renvert, Cecilia Widén, Kimmo Rumpunen
    Abstract:

    Aim of study The present study was undertaken to evaluate the effects from different leaf extracts of the traditional medicinal herb Plantago Major L. (plantain) on cell proliferation and migration ...

Baris Kaki - One of the best experts on this subject based on the ideXlab platform.

  • Plantago Major protective effects on antioxidant status after administration of 7 12 dimethylbenz a anthracene in rats
    Asian Pacific Journal of Cancer Prevention, 2011
    Co-Authors: Gokhan Oto, Suat Ekin, Hulya Ozdemir, Halit Demir, Semih Yasar, Abdulkadir Levent, Ismet Berber, Baris Kaki
    Abstract:

    Aim: The present study was designed to evaluate effects of Plantago Major extract on oxidative status in Wistar albino rats administrated 7,12-dimethylbenz(a)anthracene (DMBA). Methods: Rats were divided into three equal groups of 6 animals each: Group 1 controls, group 2 treated with DMBA (100 mg/kg, single dose) and group 3 receiving the DMBA and the aqueous extract at 100 mg/kg/d for 60 days. Results: Significant decrease in catalase (P<0.05), carbonic anhydrase (p≤0.01), reduced glutathione (GSH) (P<0.01) and total protein (P<0.01) values was observed in the DMBA group compared with the healthy controls and DMBA + Plantago Major groups. Conclusion: The results suggest preventive effects of Plantago Major on DMBA induced oxidative damage in Wistar albino rats that might be due to decreased free radical generation.

  • Plantago Major protective effects on antioxidant status after administration of 7,12-Dimethylbenz(a)anthracene in rats.
    Asian Pacific journal of cancer prevention : APJCP, 2011
    Co-Authors: Gokhan Oto, Suat Ekin, Hulya Ozdemir, Halit Demir, Semih Yasar, Abdulkadir Levent, Ismet Berber, Baris Kaki
    Abstract:

    Aim: The present study was designed to evaluate effects of Plantago Major extract on oxidative status in Wistar albino rats administrated 7,12-dimethylbenz(a)anthracene (DMBA). Methods: Rats were divided into three equal groups of 6 animals each: Group 1 controls, group 2 treated with DMBA (100 mg/kg, single dose) and group 3 receiving the DMBA and the aqueous extract at 100 mg/kg/d for 60 days. Results: Significant decrease in catalase (P