The Experts below are selected from a list of 168 Experts worldwide ranked by ideXlab platform

Hongfu Zhou - One of the best experts on this subject based on the ideXlab platform.

  • development of a neutral Embedding resin for optical imaging of fluorescently labeled biological tissue
    Journal of Biomedical Optics, 2017
    Co-Authors: Hongfu Zhou, Yumiao Xiong, Yadong Gang, Longhui Li, Shenghua Chen, Yu Wang, Shaoqun Zeng
    Abstract:

    : Plastic Embedding is widely applied in light microscopy analyses. Previous studies have shown that Embedding agents and related techniques can greatly affect the quality of biological tissue Embedding and fluorescent imaging. Specifically, it is difficult to preserve endogenous fluorescence using currently available acidic commercial Embedding resins and related Embedding techniques directly. Here, we developed a neutral Embedding resin that improved the green fluorescent protein (GFP), yellow fluorescent protein (YFP), and DsRed fluorescent intensity without adjusting the pH value of monomers or reactivating fluorescence in lye. The Embedding resin had a high degree of polymerization, and its fluorescence preservation ratios for GFP, YFP, and DsRed were 126.5%, 155.8%, and 218.4%, respectively.

  • Plastic Embedding immunolabeled large-volume samples for three-dimensional high-resolution imaging
    Biomedical Optics Express, 2017
    Co-Authors: Yadong Gang, Xiuli Liu, Yao Jia, Ruixi Chen, Hongfu Zhou, Xiaojun Wang, Fangfang Yin, Qi Zhang, Ling Liu, Gong Rao
    Abstract:

    High-resolution three-dimensional biomolecule distribution information of large samples is essential to understanding their biological structure and function. Here, we proposed a method combining large sample resin Embedding with iDISCO immunofluorescence staining to acquire the profile of biomolecules with high spatial resolution. We evaluated the compatibility of Plastic Embedding with an iDISCO staining technique and found that the fluorophores and the neuronal fine structures could be well preserved in the Lowicryl HM20 resin, and that numerous antibodies and fluorescent tracers worked well upon Lowicryl HM20 resin Embedding. Further, using fluorescence Micro- Optical sectioning tomography (fMOST) technology combined with ultra-thin slicing and imaging, we were able to image the immunolabeled large-volume tissues with high resolution.

Yadong Gang - One of the best experts on this subject based on the ideXlab platform.

  • development of a neutral Embedding resin for optical imaging of fluorescently labeled biological tissue
    Journal of Biomedical Optics, 2017
    Co-Authors: Hongfu Zhou, Yumiao Xiong, Yadong Gang, Longhui Li, Shenghua Chen, Yu Wang, Shaoqun Zeng
    Abstract:

    : Plastic Embedding is widely applied in light microscopy analyses. Previous studies have shown that Embedding agents and related techniques can greatly affect the quality of biological tissue Embedding and fluorescent imaging. Specifically, it is difficult to preserve endogenous fluorescence using currently available acidic commercial Embedding resins and related Embedding techniques directly. Here, we developed a neutral Embedding resin that improved the green fluorescent protein (GFP), yellow fluorescent protein (YFP), and DsRed fluorescent intensity without adjusting the pH value of monomers or reactivating fluorescence in lye. The Embedding resin had a high degree of polymerization, and its fluorescence preservation ratios for GFP, YFP, and DsRed were 126.5%, 155.8%, and 218.4%, respectively.

  • Plastic Embedding immunolabeled large-volume samples for three-dimensional high-resolution imaging
    Biomedical Optics Express, 2017
    Co-Authors: Yadong Gang, Xiuli Liu, Yao Jia, Ruixi Chen, Hongfu Zhou, Xiaojun Wang, Fangfang Yin, Qi Zhang, Ling Liu, Gong Rao
    Abstract:

    High-resolution three-dimensional biomolecule distribution information of large samples is essential to understanding their biological structure and function. Here, we proposed a method combining large sample resin Embedding with iDISCO immunofluorescence staining to acquire the profile of biomolecules with high spatial resolution. We evaluated the compatibility of Plastic Embedding with an iDISCO staining technique and found that the fluorophores and the neuronal fine structures could be well preserved in the Lowicryl HM20 resin, and that numerous antibodies and fluorescent tracers worked well upon Lowicryl HM20 resin Embedding. Further, using fluorescence Micro- Optical sectioning tomography (fMOST) technology combined with ultra-thin slicing and imaging, we were able to image the immunolabeled large-volume tissues with high resolution.

Srećko Gajović - One of the best experts on this subject based on the ideXlab platform.

  • Semi-thin sections of epoxy resin-embedded mouse embryos in morphological analysis of whole mount in situ RNA hybridization
    Journal of microscopy, 2008
    Co-Authors: Dinko Mitrečić, Vesna Furić Čunko, Srećko Gajović
    Abstract:

    Summary Descriptive morphological studies are often combined with gene expression pattern analyses. Unembedded vibratome or cryotome sections are compatible with in situ RNA hybridization, but spatial resolution is rather low for precise microscopic studies necessary in embryology. Therefore, use of Plastic Embedding media, which allow semi-thin and ultra-thin sectioning for light and electron microscopy, could be an important advantage. This work suggested a new approach based on the whole mount hybridization of mouse embryos and subsequent epoxy resin Embedding. Epoxy resin allowed serial sectioning of semi-thin sections with preserved in situ RNA hybridization signal, which was a necessary prerequisite for precise morphological analysis of embryo development.

Shaoqun Zeng - One of the best experts on this subject based on the ideXlab platform.

  • development of a neutral Embedding resin for optical imaging of fluorescently labeled biological tissue
    Journal of Biomedical Optics, 2017
    Co-Authors: Hongfu Zhou, Yumiao Xiong, Yadong Gang, Longhui Li, Shenghua Chen, Yu Wang, Shaoqun Zeng
    Abstract:

    : Plastic Embedding is widely applied in light microscopy analyses. Previous studies have shown that Embedding agents and related techniques can greatly affect the quality of biological tissue Embedding and fluorescent imaging. Specifically, it is difficult to preserve endogenous fluorescence using currently available acidic commercial Embedding resins and related Embedding techniques directly. Here, we developed a neutral Embedding resin that improved the green fluorescent protein (GFP), yellow fluorescent protein (YFP), and DsRed fluorescent intensity without adjusting the pH value of monomers or reactivating fluorescence in lye. The Embedding resin had a high degree of polymerization, and its fluorescence preservation ratios for GFP, YFP, and DsRed were 126.5%, 155.8%, and 218.4%, respectively.

Giselle Thibaudeau - One of the best experts on this subject based on the ideXlab platform.

  • Laser microdissection of semi-thin sections from Plastic-embedded tissue for studying plant–organism developmental processes at single-cell resolution
    Journal of Plant Interactions, 2014
    Co-Authors: Vincent P Klink, Giselle Thibaudeau
    Abstract:

    The ability to analyze the gene activity occurring within a single cell has ushered in a new understanding of complex biological processes. Furthermore, this capability has established the prerequisite technologies for the analysis of cells involved in complex pathogenic and/or symbiotic interactions. Collectively, the identification of biological models permitting the analysis of individual cells and improvements in histological technology are allowing for analyses of cells positioned within tissues and involved in complex cellular interactions at unprecedented resolution. Here, a Plastic Embedding procedure is used for laser microdissection of plant tissues infected with a pathogen. This technique enabled the acquisition of nucleic acids from semi-thin sections that can be used for downstream biological studies of host–pathogen interaction at the single-cell resolution.