The Experts below are selected from a list of 195 Experts worldwide ranked by ideXlab platform
Gong Yu-xiang - One of the best experts on this subject based on the ideXlab platform.
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Detection of Pneumocystis Carinii on the lung paraffin section by RNA in situ hybridization
Journal of Tropical Medicine, 2011Co-Authors: Gong Yu-xiangAbstract:Objective To investigate the possible application of RNA in situ hybridization for the detection of Pneumocystis Carinii. Methods The Pneumocystis Carinii infected Wistar rat model was established. Rats inoculated with Pneumocystis Carinii were sacrificed 3, 4, 5, 6, 7, 8, 9 and 10 weeks after injection with Dexanethasone, and the paraffin sections of lung were prepared to perform the in situ hybridization with Dig-labeled oligonucleotide probe complememtary to small subunit rRNA of Pneumocystis Carinii. Results It was found that the specific positive results were observed on the paraffin sections 3 weeks after injection.All the 32 paraffin sections detected by in situ hybridization demonstrated positive results,while only 25 sections showed positive results by Wright-Giemsa's staining. Conclusion The RNA in situ hybridization is a sensitive and specific method for the detection of Pneumocystis Carinii in pathological sections.
Zeng Xia - One of the best experts on this subject based on the ideXlab platform.
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Establishment and Analysis of the Morphological Database and Reference Paramenter of Pneumocystis Carinii Cysts
Journal of Tropical Medicine, 2004Co-Authors: Zeng XiaAbstract:Objective To establish and analyze the morphological data and refe rence parameters of the Pneumocystis Carinii cysts. Methods The long diameter,s hort diameter, perimeter and equivalent diameter of Pneumocystis Carinii cysts w ere measured by computer image analysis system. The sphere grade, area and volum e of cysts and numbers of mature cysts were calculated. Result The long diameter range from 8.31μm to 2.31μm;short diameter range from 6.30μm to 1.69μm;vo lume range from164.73μm3 to 4.64μm3. Conclusion The morphological data and par ameters of Pneumocystis Carinii cysts are potentially useful for evaluating its morphology.
Naseem A. Ansari - One of the best experts on this subject based on the ideXlab platform.
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Pneumocystis Carinii Infection
Archives of Pathology & Laboratory Medicine, 2004Co-Authors: Javed F. Wazir, Naseem A. AnsariAbstract:Abstract Objective.—To review and update the literature on current trends with regard to Pneumocystis Carinii (jiroveci ) diagnosis, treatment modalities, and its role in human disease processes. Data Sources.—Bibliographic databases (PubMed and Ovid) were searched for material and data between 1980 and September 2003 relevant to the review. Indexing terms used were “Pneumocystis Carinii pneumonia,” and “Pneumocystis jiroveci,” with the English language as a constraint. Other sources were the PhD thesis of one of the authors (J.F.W., London University, 1993) and the library at the Arabian Gulf University in the Kingdom of Bahrain. Study Selection.—Acquired immunodeficiency syndrome and organ transplant cases with Pneumocystis Carinii pneumonia. Data Extraction.—Independent extraction by 2 observers. Data Synthesis.—We reviewed the major characteristics of P Carinii (jiroveci ) with special emphasis on the more recently acquired data including the presence of a round pore in the cyst wall, which appears to...
Michael P. Mullen - One of the best experts on this subject based on the ideXlab platform.
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Pleural Pneumocystis Carinii infection.
Chest, 1991Co-Authors: Peter Mariuz, Mario Raviglione, Ira A. Gould, Michael P. MullenAbstract:Extrapulmonary Pneumocystis Carinii infection is a rare occurrence in patients with AIDS. Pleural involvement has been demonstrated in only one case, and this occurred after pneumothorax. This is a case report of pleural pneumocystosis in a patient with AIDS who did not have a pneumothorax. (Chest 1991; 99:774–76)
Robert F. Miller - One of the best experts on this subject based on the ideXlab platform.
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Pneumocystis Carinii infection in non-AIDS patients.
Current opinion in infectious diseases, 1999Co-Authors: Robert F. MillerAbstract:Infection with the opportunist fungus Pneumocystis Carinii remains a significant cause of morbidity and mortality in non-HIV-infected individuals immunosuppressed by a wide range of malignancies, transplantation and inflammatory conditions. Glucocorticoid use appears to be an independent risk factor for the development of Pneumocystis Carinii pneumonia. Transmission from infected to susceptible patients may occur, albeit infrequently. A diagnosis of Pneumocystis Carinii pneumonia may be achieved in the majority of cases by DNA detection using polymerase chain reaction on oropharyngeal mouth washes.