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Mark Estelle - One of the best experts on this subject based on the ideXlab platform.
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root ultraviolet b sensitive1 weak Auxin response3 is essential for Polar Auxin Transport in arabidopsis
Plant Physiology, 2013Co-Authors: Michael Karampelias, Jerry D Cohen, Angus S Murphy, Jiri Friml, Stéphanie Robert, Wendy Ann Peer, Ranjan Swarup, Mark EstelleAbstract:The phytohormone Auxin regulates virtually every aspect of plant development. To identify new genes involved in Auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the Auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak Auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression when treated with the synthetic Auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases Polar Auxin Transport and results in a disruption of the asymmetric Auxin distribution. The levels of the Auxin Transporters Auxin1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of Polar Auxin Transport by maintaining the proper level of Auxin Transporters on the plasma membrane.
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arabidopsis root uvb sensitive2 weak Auxin response1 is required for Polar Auxin Transport
The Plant Cell, 2010Co-Authors: Wendy Ann Peer, Jerry D Cohen, Angus S Murphy, Jiri Friml, Stéphanie Robert, Ranjan Swarup, Michael J. Prigge, Ding Tang, Mark EstelleAbstract:Auxin is an essential phytohormone that regulates many aspects of plant development. To identify new genes that function in Auxin signaling, we performed a genetic screen for Arabidopsis thaliana mutants with an alteration in the expression of the Auxin-responsive reporter DR5rev:GFP (for green fluorescent protein). One of the mutants recovered in this screen, called weak Auxin response1 (wxr1), has a defect in Auxin response and exhibits a variety of Auxin-related growth defects in the root. Polar Auxin Transport is reduced in wxr1 seedlings, resulting in Auxin accumulation in the hypocotyl and cotyledons and a reduction in Auxin levels in the root apex. In addition, the levels of the PIN Auxin Transport proteins are reduced in the wxr1 root. We also show that WXR1 is ROOT UV-B SENSITIVE2 (RUS2), a member of the broadly conserved DUF647 domain protein family found in diverse eukaryotic organisms. Our data indicate that RUS2/WXR1 is required for Auxin Transport and to maintain the normal levels of PIN proteins in the root.
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big a calossin like protein required for Polar Auxin Transport in arabidopsis
Genes & Development, 2001Co-Authors: Elizabeth Dewey, Klaus Palme, Mark Estelle, Jiri Friml, Yunde Zhao, Kimberley C Snowden, Joanna Putterill, Joanne ChoryAbstract:Polar Auxin Transport is crucial for the regulation of Auxin action and required for some light-regulated responses during plant development. We have found that two mutants of Arabidopsis—doc1, which displays altered expression of light-regulated genes, and tir3, known for its reduced Auxin Transport—have similar defects and define mutations in a single gene that we have renamed BIG. BIG is very similar to the Drosophila gene Calossin/Pushover, a member of a gene family also present in Caenorhabditis elegans and human genomes. The protein encoded by BIG is extraordinary in size, 560 kD, and contains several putative Zn-finger domains. Expression-profiling experiments indicate that altered expression of multiple light-regulated genes in doc1 mutants can be suppressed by elevated levels of Auxin caused by overexpression of an Auxin biosynthetic gene, suggesting that normal Auxin distribution is required to maintain low-level expression of these genes in the dark. Double mutants of tir3 with the Auxin mutants pin1, pid, and axr1 display severe defects in Auxin-dependent growth of the inflorescence. Chemical inhibitors of Auxin Transport change the intracellular localization of the Auxin efflux carrier PIN1 in doc1/tir3 mutants, supporting the idea that BIG is required for normal Auxin efflux.
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Polar Auxin Transport: New Support for an Old Model
The Plant Cell, 1998Co-Authors: Mark EstelleAbstract:In 1880, Charles Darwin noted that “some influence,” later shown to be indole-3-acetic acid (IAA), moves from the tip of an oat coleoptile to the region below the tip, where it controls elongation ([Darwin, 1880][1]). Darwin's statement was probably the first description of Polar Auxin Transport
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reduced naphthylphthalamic acid binding in the tir3 mutant of arabidopsis is associated with a reduction in Polar Auxin Transport and diverse morphological defects
The Plant Cell, 1997Co-Authors: Max Ruegger, Gloria K Muday, Elizabeth Dewey, Lawrence Hobbie, Dana E Brown, Paul Bernasconi, Jocelyn C Turner, Mark EstelleAbstract:Polar Auxin Transport plays a key role in the regulation of plant growth and development. To identify genes involved in this process, we have developed a genetic procedure to screen for mutants of Arabidopsis that are altered in their response to Auxin Transport inhibitors. We recovered a total of 16 independent mutants that defined seven genes, called Transport INHIBITOR RESPONSE (TIR) genes. Recessive mutations in one of these genes, TIR3, result in altered responses to Transport inhibitors, a reduction in Polar Auxin Transport, and a variety of morphological defects that can be ascribed to changes in indole-3-acetic acid distribution. Most dramatically, tir3 seedlings are strongly deficient in lateral root production, a process that is known to depend on Polar Auxin Transport from the shoot into the root. In addition, tir3 plants display a reduction in apical dominance as well as decreased elongation of siliques, pedicels, roots, and the inflorescence. Biochemical studies indicate that tir3 plants have a reduced number of N-1-naphthylphthalamic (NPA) binding sites, suggesting that the TIR3 gene is required for expression, localization, or stabilization of the NPA binding protein (NBP). Alternatively, the TIR3 gene may encode the NBP. Because the tir3 mutants have a substantial defect in NPA binding, their phenotype provides genetic evidence for a role for the NBP in plant growth and development.
Jiri Friml - One of the best experts on this subject based on the ideXlab platform.
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root ultraviolet b sensitive1 weak Auxin response3 is essential for Polar Auxin Transport in arabidopsis
Plant Physiology, 2013Co-Authors: Michael Karampelias, Jerry D Cohen, Angus S Murphy, Jiri Friml, Stéphanie Robert, Wendy Ann Peer, Ranjan Swarup, Mark EstelleAbstract:The phytohormone Auxin regulates virtually every aspect of plant development. To identify new genes involved in Auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the Auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak Auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression when treated with the synthetic Auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases Polar Auxin Transport and results in a disruption of the asymmetric Auxin distribution. The levels of the Auxin Transporters Auxin1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of Polar Auxin Transport by maintaining the proper level of Auxin Transporters on the plasma membrane.
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ROOT ULTRAVIOLET B-SENSITIVE1/WEAK Auxin RESPONSE3 Is Essential for Polar Auxin Transport in Arabidopsis
Plant Physiology, 2013Co-Authors: Michael Karampelias, Jerry D Cohen, Angus S Murphy, Stéphanie Robert, Wendy Ann Peer, Ranjan Swarup, Jiri FrimlAbstract:The phytohormone Auxin regulates virtually every aspect of plant development. To identify new genes involved in Auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the Auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak Auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression when treated with the synthetic Auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases Polar Auxin Transport and results in a disruption of the asymmetric Auxin distribution. The levels of the Auxin Transporters Auxin1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of Polar Auxin Transport by maintaining the proper level of Auxin Transporters on the plasma membrane.
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a major facilitator superfamily Transporter plays a dual role in Polar Auxin Transport and drought stress tolerance in arabidopsis
The Plant Cell, 2013Co-Authors: Estelle Remy, Pawel Radoslaw Baster, Jiri Friml, Tânia R Cabrito, Rita A Batista, Miguel C Teixeira, Isabel Sacorreia, Paula DuqueAbstract:Many key aspects of plant development are regulated by the Polarized Transport of the phytohormone Auxin. Cellular Auxin efflux, the rate-limiting step in this process, has been shown to rely on the coordinated action of PIN-formed (PIN) and B-type ATP binding cassette (ABCB) carriers. Here, we report that Polar Auxin Transport in the Arabidopsis thaliana root also requires the action of a Major Facilitator Superfamily (MFS) Transporter, Zinc-Induced Facilitator-Like 1 (ZIFL1). Sequencing, promoter-reporter, and fluorescent protein fusion experiments indicate that the full-length ZIFL1.1 protein and a truncated splice isoform, ZIFL1.3, localize to the tonoplast of root cells and the plasma membrane of leaf stomatal guard cells, respectively. Using reverse genetics, we show that the ZIFL1.1 Transporter regulates various root Auxin-related processes, while the ZIFL1.3 isoform mediates drought tolerance by regulating stomatal closure. Auxin Transport and immunolocalization assays demonstrate that ZIFL1.1 indirectly modulates cellular Auxin efflux during shootward Auxin Transport at the root tip, likely by regulating plasma membrane PIN2 abundance. Finally, heterologous expression in yeast revealed that ZIFL1.1 and ZIFL1.3 share H+-coupled K+ Transport activity. Thus, by determining the subcellular and tissue distribution of two isoforms, alternative splicing dictates a dual function for the ZIFL1 Transporter. We propose that this MFS carrier regulates stomatal movements and Polar Auxin Transport by modulating potassium and proton fluxes in Arabidopsis cells.
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ZIFL1.1 Transporter modulates Polar Auxin Transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip
Plant Signaling & Behavior, 2013Co-Authors: Estell Remy, Pawel Radoslaw Baster, Jiri Friml, Paula DuqueAbstract:Cell-to-cell directional flow of the phytohormone Auxin is primarily established by Polar localization of the PIN Auxin Transporters, a process tightly regulated at multiple levels by Auxin itself. We recently reported that, in the context of strong Auxin flows, activity of the vacuolar ZIFL1.1 Transporter is required for fine-tuning of Polar Auxin Transport rates in the Arabidopsis root. In particular, ZIFL1.1 function protects plasma-membrane stability of the PIN2 carrier in epidermal root tip cells under conditions normally triggering PIN2 degradation. Here, we show that ZIFL1.1 activity at the root tip also promotes PIN1 plasma-membrane abundance in central cylinder cells, thus supporting the notion that ZIFL1.1 acts as a general positive modulator of Polar Auxin Transport in roots.
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Arabidopsis ROOT UVB SENSITIVE2/WEAK Auxin RESPONSE1 is required for Polar Auxin Transport.
The Plant Cell, 2010Co-Authors: Wendy Ann Peer, Jerry D Cohen, Angus S Murphy, Jiri Friml, Stéphanie Robert, Ranjan Swarup, Michael J. Prigge, Ding TangAbstract:Auxin is an essential phytohormone that regulates many aspects of plant development. To identify new genes that function in Auxin signaling, we performed a genetic screen for Arabidopsis thaliana mutants with an alteration in the expression of the Auxin-responsive reporter DR5rev:GFP (for green fluorescent protein). One of the mutants recovered in this screen, called weak Auxin response1 (wxr1), has a defect in Auxin response and exhibits a variety of Auxin-related growth defects in the root. Polar Auxin Transport is reduced in wxr1 seedlings, resulting in Auxin accumulation in the hypocotyl and cotyledons and a reduction in Auxin levels in the root apex. In addition, the levels of the PIN Auxin Transport proteins are reduced in the wxr1 root. We also show that WXR1 is ROOT UV-B SENSITIVE2 (RUS2), a member of the broadly conserved DUF647 domain protein family found in diverse eukaryotic organisms. Our data indicate that RUS2/WXR1 is required for Auxin Transport and to maintain the normal levels of PIN proteins in the root.
Jerry D Cohen - One of the best experts on this subject based on the ideXlab platform.
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loss of gsnor1 function leads to compromised Auxin signaling and Polar Auxin Transport
Molecular Plant, 2015Co-Authors: Da Li Wang, Chao Wang, Angela Hendrickson Culler, Molly Kreiser, Jayanti Suresh, Jerry D Cohen, Barbara BakerAbstract:Abstract Cross talk between phytohormones, nitric oxide (NO), and Auxin has been implicated in the control of plant growth and development. Two recent reports indicate that NO promoted Auxin signaling but inhibited Auxin Transport probably through S-nitrosylation. However, genetic evidence for the effect of S-nitrosylation on Auxin physiology has been lacking. In this study, we used a genetic approach to understand the broader role of S-nitrosylation in Auxin physiology in Arabidopsis . We compared Auxin signaling and Transport in Col-0 and gsnor1-3 , a loss-of-function GSNOR1 mutant defective in protein de-nitrosylation. Our results showed that Auxin signaling was impaired in the gsnor1-3 mutant as revealed by significantly reduced DR5-GUS/DR5-GFP accumulation and compromised degradation of AXR3NT-GUS, a useful reporter in interrogating Auxin-mediated degradation of Aux/IAA by Auxin receptors. In addition, Polar Auxin Transport was compromised in gsnor1-3 , which was correlated with universally reduced levels of PIN or GFP-PIN proteins in the roots of the mutant in a manner independent of transcription and 26S proteasome degradation. Our results suggest that S-nitrosylation and GSNOR1-mediated de-nitrosylation contribute to Auxin physiology, and impaired Auxin signaling and compromised Auxin Transport are responsible for the Auxin-related morphological phenotypes displayed by the gsnor1-3 mutant.
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root ultraviolet b sensitive1 weak Auxin response3 is essential for Polar Auxin Transport in arabidopsis
Plant Physiology, 2013Co-Authors: Michael Karampelias, Jerry D Cohen, Angus S Murphy, Jiri Friml, Stéphanie Robert, Wendy Ann Peer, Ranjan Swarup, Mark EstelleAbstract:The phytohormone Auxin regulates virtually every aspect of plant development. To identify new genes involved in Auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the Auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak Auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression when treated with the synthetic Auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases Polar Auxin Transport and results in a disruption of the asymmetric Auxin distribution. The levels of the Auxin Transporters Auxin1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of Polar Auxin Transport by maintaining the proper level of Auxin Transporters on the plasma membrane.
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ROOT ULTRAVIOLET B-SENSITIVE1/WEAK Auxin RESPONSE3 Is Essential for Polar Auxin Transport in Arabidopsis
Plant Physiology, 2013Co-Authors: Michael Karampelias, Jerry D Cohen, Angus S Murphy, Stéphanie Robert, Wendy Ann Peer, Ranjan Swarup, Jiri FrimlAbstract:The phytohormone Auxin regulates virtually every aspect of plant development. To identify new genes involved in Auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the Auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak Auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression when treated with the synthetic Auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases Polar Auxin Transport and results in a disruption of the asymmetric Auxin distribution. The levels of the Auxin Transporters Auxin1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of Polar Auxin Transport by maintaining the proper level of Auxin Transporters on the plasma membrane.
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Arabidopsis ROOT UVB SENSITIVE2/WEAK Auxin RESPONSE1 is required for Polar Auxin Transport.
The Plant Cell, 2010Co-Authors: Wendy Ann Peer, Jerry D Cohen, Angus S Murphy, Jiri Friml, Stéphanie Robert, Ranjan Swarup, Michael J. Prigge, Ding TangAbstract:Auxin is an essential phytohormone that regulates many aspects of plant development. To identify new genes that function in Auxin signaling, we performed a genetic screen for Arabidopsis thaliana mutants with an alteration in the expression of the Auxin-responsive reporter DR5rev:GFP (for green fluorescent protein). One of the mutants recovered in this screen, called weak Auxin response1 (wxr1), has a defect in Auxin response and exhibits a variety of Auxin-related growth defects in the root. Polar Auxin Transport is reduced in wxr1 seedlings, resulting in Auxin accumulation in the hypocotyl and cotyledons and a reduction in Auxin levels in the root apex. In addition, the levels of the PIN Auxin Transport proteins are reduced in the wxr1 root. We also show that WXR1 is ROOT UV-B SENSITIVE2 (RUS2), a member of the broadly conserved DUF647 domain protein family found in diverse eukaryotic organisms. Our data indicate that RUS2/WXR1 is required for Auxin Transport and to maintain the normal levels of PIN proteins in the root.
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arabidopsis root uvb sensitive2 weak Auxin response1 is required for Polar Auxin Transport
The Plant Cell, 2010Co-Authors: Wendy Ann Peer, Jerry D Cohen, Angus S Murphy, Jiri Friml, Stéphanie Robert, Ranjan Swarup, Michael J. Prigge, Ding Tang, Mark EstelleAbstract:Auxin is an essential phytohormone that regulates many aspects of plant development. To identify new genes that function in Auxin signaling, we performed a genetic screen for Arabidopsis thaliana mutants with an alteration in the expression of the Auxin-responsive reporter DR5rev:GFP (for green fluorescent protein). One of the mutants recovered in this screen, called weak Auxin response1 (wxr1), has a defect in Auxin response and exhibits a variety of Auxin-related growth defects in the root. Polar Auxin Transport is reduced in wxr1 seedlings, resulting in Auxin accumulation in the hypocotyl and cotyledons and a reduction in Auxin levels in the root apex. In addition, the levels of the PIN Auxin Transport proteins are reduced in the wxr1 root. We also show that WXR1 is ROOT UV-B SENSITIVE2 (RUS2), a member of the broadly conserved DUF647 domain protein family found in diverse eukaryotic organisms. Our data indicate that RUS2/WXR1 is required for Auxin Transport and to maintain the normal levels of PIN proteins in the root.
Wendy Ann Peer - One of the best experts on this subject based on the ideXlab platform.
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root ultraviolet b sensitive1 weak Auxin response3 is essential for Polar Auxin Transport in arabidopsis
Plant Physiology, 2013Co-Authors: Michael Karampelias, Jerry D Cohen, Angus S Murphy, Jiri Friml, Stéphanie Robert, Wendy Ann Peer, Ranjan Swarup, Mark EstelleAbstract:The phytohormone Auxin regulates virtually every aspect of plant development. To identify new genes involved in Auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the Auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak Auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression when treated with the synthetic Auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases Polar Auxin Transport and results in a disruption of the asymmetric Auxin distribution. The levels of the Auxin Transporters Auxin1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of Polar Auxin Transport by maintaining the proper level of Auxin Transporters on the plasma membrane.
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ROOT ULTRAVIOLET B-SENSITIVE1/WEAK Auxin RESPONSE3 Is Essential for Polar Auxin Transport in Arabidopsis
Plant Physiology, 2013Co-Authors: Michael Karampelias, Jerry D Cohen, Angus S Murphy, Stéphanie Robert, Wendy Ann Peer, Ranjan Swarup, Jiri FrimlAbstract:The phytohormone Auxin regulates virtually every aspect of plant development. To identify new genes involved in Auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the Auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak Auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression when treated with the synthetic Auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases Polar Auxin Transport and results in a disruption of the asymmetric Auxin distribution. The levels of the Auxin Transporters Auxin1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of Polar Auxin Transport by maintaining the proper level of Auxin Transporters on the plasma membrane.
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Arabidopsis ROOT UVB SENSITIVE2/WEAK Auxin RESPONSE1 is required for Polar Auxin Transport.
The Plant Cell, 2010Co-Authors: Wendy Ann Peer, Jerry D Cohen, Angus S Murphy, Jiri Friml, Stéphanie Robert, Ranjan Swarup, Michael J. Prigge, Ding TangAbstract:Auxin is an essential phytohormone that regulates many aspects of plant development. To identify new genes that function in Auxin signaling, we performed a genetic screen for Arabidopsis thaliana mutants with an alteration in the expression of the Auxin-responsive reporter DR5rev:GFP (for green fluorescent protein). One of the mutants recovered in this screen, called weak Auxin response1 (wxr1), has a defect in Auxin response and exhibits a variety of Auxin-related growth defects in the root. Polar Auxin Transport is reduced in wxr1 seedlings, resulting in Auxin accumulation in the hypocotyl and cotyledons and a reduction in Auxin levels in the root apex. In addition, the levels of the PIN Auxin Transport proteins are reduced in the wxr1 root. We also show that WXR1 is ROOT UV-B SENSITIVE2 (RUS2), a member of the broadly conserved DUF647 domain protein family found in diverse eukaryotic organisms. Our data indicate that RUS2/WXR1 is required for Auxin Transport and to maintain the normal levels of PIN proteins in the root.
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arabidopsis root uvb sensitive2 weak Auxin response1 is required for Polar Auxin Transport
The Plant Cell, 2010Co-Authors: Wendy Ann Peer, Jerry D Cohen, Angus S Murphy, Jiri Friml, Stéphanie Robert, Ranjan Swarup, Michael J. Prigge, Ding Tang, Mark EstelleAbstract:Auxin is an essential phytohormone that regulates many aspects of plant development. To identify new genes that function in Auxin signaling, we performed a genetic screen for Arabidopsis thaliana mutants with an alteration in the expression of the Auxin-responsive reporter DR5rev:GFP (for green fluorescent protein). One of the mutants recovered in this screen, called weak Auxin response1 (wxr1), has a defect in Auxin response and exhibits a variety of Auxin-related growth defects in the root. Polar Auxin Transport is reduced in wxr1 seedlings, resulting in Auxin accumulation in the hypocotyl and cotyledons and a reduction in Auxin levels in the root apex. In addition, the levels of the PIN Auxin Transport proteins are reduced in the wxr1 root. We also show that WXR1 is ROOT UV-B SENSITIVE2 (RUS2), a member of the broadly conserved DUF647 domain protein family found in diverse eukaryotic organisms. Our data indicate that RUS2/WXR1 is required for Auxin Transport and to maintain the normal levels of PIN proteins in the root.
Angus S Murphy - One of the best experts on this subject based on the ideXlab platform.
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root ultraviolet b sensitive1 weak Auxin response3 is essential for Polar Auxin Transport in arabidopsis
Plant Physiology, 2013Co-Authors: Michael Karampelias, Jerry D Cohen, Angus S Murphy, Jiri Friml, Stéphanie Robert, Wendy Ann Peer, Ranjan Swarup, Mark EstelleAbstract:The phytohormone Auxin regulates virtually every aspect of plant development. To identify new genes involved in Auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the Auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak Auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression when treated with the synthetic Auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases Polar Auxin Transport and results in a disruption of the asymmetric Auxin distribution. The levels of the Auxin Transporters Auxin1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of Polar Auxin Transport by maintaining the proper level of Auxin Transporters on the plasma membrane.
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ROOT ULTRAVIOLET B-SENSITIVE1/WEAK Auxin RESPONSE3 Is Essential for Polar Auxin Transport in Arabidopsis
Plant Physiology, 2013Co-Authors: Michael Karampelias, Jerry D Cohen, Angus S Murphy, Stéphanie Robert, Wendy Ann Peer, Ranjan Swarup, Jiri FrimlAbstract:The phytohormone Auxin regulates virtually every aspect of plant development. To identify new genes involved in Auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the Auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak Auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression when treated with the synthetic Auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases Polar Auxin Transport and results in a disruption of the asymmetric Auxin distribution. The levels of the Auxin Transporters Auxin1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of Polar Auxin Transport by maintaining the proper level of Auxin Transporters on the plasma membrane.
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the exocyst complex contributes to pin Auxin efflux carrier recycling and Polar Auxin Transport in arabidopsis
Plant Journal, 2013Co-Authors: Edita Jankova Drdova, Lukas Synek, Tamara Pecenkova, Michal Hala, Ivan Kulich, John E Fowler, Angus S Murphy, Viktor žarskýAbstract:: In land plants Polar Auxin Transport is one of the substantial processes guiding whole plant Polarity and morphogenesis. Directional Auxin fluxes are mediated by PIN Auxin efflux carriers, Polarly localized at the plasma membrane. The Polarization of exocytosis in yeast and animals is assisted by the exocyst: an octameric vesicle-tethering complex and an effector of Rab and Rho GTPases. Here we show that rootward Polar Auxin Transport is compromised in roots of Arabidopsis thaliana loss-of-function mutants in the EXO70A1 exocyst subunit. The recycling of PIN1 and PIN2 proteins from brefeldin-A compartments is delayed after the brefeldin-A washout in exo70A1 and sec8 exocyst mutants. Relocalization of PIN1 and PIN2 proteins after prolonged brefeldin-A treatment is largely impaired in these mutants. At the same time, however, plasma membrane localization of GFP:EXO70A1, and the other exocyst subunits studied (GFP:SEC8 and YFP:SEC10), is resistant to brefeldin-A treatment. In root cells of the exo70A1 mutant, a portion of PIN2 is internalized and retained in specific, abnormally enlarged, endomembrane compartments that are distinct from VHA-a1-labelled early endosomes or the trans-Golgi network, but are RAB-A5d positive. We conclude that the exocyst is involved in PIN1 and PIN2 recycling, and thus in Polar Auxin Transport regulation.
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Arabidopsis ROOT UVB SENSITIVE2/WEAK Auxin RESPONSE1 is required for Polar Auxin Transport.
The Plant Cell, 2010Co-Authors: Wendy Ann Peer, Jerry D Cohen, Angus S Murphy, Jiri Friml, Stéphanie Robert, Ranjan Swarup, Michael J. Prigge, Ding TangAbstract:Auxin is an essential phytohormone that regulates many aspects of plant development. To identify new genes that function in Auxin signaling, we performed a genetic screen for Arabidopsis thaliana mutants with an alteration in the expression of the Auxin-responsive reporter DR5rev:GFP (for green fluorescent protein). One of the mutants recovered in this screen, called weak Auxin response1 (wxr1), has a defect in Auxin response and exhibits a variety of Auxin-related growth defects in the root. Polar Auxin Transport is reduced in wxr1 seedlings, resulting in Auxin accumulation in the hypocotyl and cotyledons and a reduction in Auxin levels in the root apex. In addition, the levels of the PIN Auxin Transport proteins are reduced in the wxr1 root. We also show that WXR1 is ROOT UV-B SENSITIVE2 (RUS2), a member of the broadly conserved DUF647 domain protein family found in diverse eukaryotic organisms. Our data indicate that RUS2/WXR1 is required for Auxin Transport and to maintain the normal levels of PIN proteins in the root.
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arabidopsis root uvb sensitive2 weak Auxin response1 is required for Polar Auxin Transport
The Plant Cell, 2010Co-Authors: Wendy Ann Peer, Jerry D Cohen, Angus S Murphy, Jiri Friml, Stéphanie Robert, Ranjan Swarup, Michael J. Prigge, Ding Tang, Mark EstelleAbstract:Auxin is an essential phytohormone that regulates many aspects of plant development. To identify new genes that function in Auxin signaling, we performed a genetic screen for Arabidopsis thaliana mutants with an alteration in the expression of the Auxin-responsive reporter DR5rev:GFP (for green fluorescent protein). One of the mutants recovered in this screen, called weak Auxin response1 (wxr1), has a defect in Auxin response and exhibits a variety of Auxin-related growth defects in the root. Polar Auxin Transport is reduced in wxr1 seedlings, resulting in Auxin accumulation in the hypocotyl and cotyledons and a reduction in Auxin levels in the root apex. In addition, the levels of the PIN Auxin Transport proteins are reduced in the wxr1 root. We also show that WXR1 is ROOT UV-B SENSITIVE2 (RUS2), a member of the broadly conserved DUF647 domain protein family found in diverse eukaryotic organisms. Our data indicate that RUS2/WXR1 is required for Auxin Transport and to maintain the normal levels of PIN proteins in the root.
